Chitosan and chitosan derivatives are biomaterials of great scientific interest for biomedical applications although some of the properties are limited as their solubility in the aqueous medium. N-heterocyclic compounds are of interest for chitosan alkylation and new derivatives can be obtained by modification of his structure resulting thus new structures of biomedical interest Chitosan samples from two sources were analysed, in which the dissociation is favoured in the aqueous medium by the presence of a bipyridine salt, N,N'-bis(phenacyl)-4,4'-bipyridinium dibromide. The synthesis of chitosan’ derivate was conducted in aqueous medium at 60 °C, by magnetic stirring for 24 hours. The dissociation is better and reduced the pH, to values lower than pH 6, which suggests the achievement of favourable structural arrangements between chitosan and dibromide bipyridinium. The FTIR spectra parameters indicate the presence of characteristic bands of the bipyridinium salt in the chitosan derivates structures, with structural differences in their composition.