Nitrile Biotransformations Research Articles

Polyvinyl alcohol-immobilized whole-cell preparations for the biotransformation of nitriles

Resting cells of Rhodococcus sp. ATCC 39484 were immobilized in polyvinyl alcohol (PVA). The PVA-beads showed nitrile-hydrolysing activity with propionitrile as a model substrate. Drying of the beads resulted in a loss of weight of 85 %. Re-swollen beads showed no loss in activity. In a continuously-operated fixed-bed bioreactor, beads were able to convert substrate concentrations up to 100 mM.

Regio- and stereo-specific nitrile hydrolysis by the nitrile hydratase fromRhodococcusAJ270

An acetonitrile-utilising bacterium AJ270 has been isolated from soil, identified as a Rhodococcus sp. and shown to be distinct from all the recognised species of the genus. It grows well on 32 of 36 aliphatic, aromatic and hetero-aromatic nitriles tested and is capable of rapid growth on high concentrations (0.25–0.38 M) of acetonitrile, benzonitrile and 3-cyanopyridine. The nitrile hydratase of Rhodococcus AJ270 is stable on storage for 18 months at − 20° C, has activity against a very broad range of nitriles and dinitriles and is able to catalyse regio-specific and stereo-specific nitrile biotransformations. The suitability of AJ270 as a robust and versatile biocatalyst is discussed.

Regio- and stereo-specific nitrile hydrolysis by the nitrile hydratase from Rhodococcus AJ270

An acetonitrile-utilising bacterium AJ270 has been isolated from soil, identified as a Rhodococcus sp. and shown to be distinct from all the recognised species of the genus. It grows well on 32 of 36 aliphatic, aromatic and hetero-aromatic nitriles tested and is capable of rapid growth on high concentrations (0.25–0.38 M) of acetonitrile, benzonitrile and 3-cyanopyridine. The nitrile hydratase of Rhodococcus AJ270 is stable on storage for 18 months at − 20° C, has activity against a very broad range of nitriles and dinitriles and is able to catalyse regio-specific and stereo-specific nitrile biotransformations. The suitability of AJ270 as a robust and versatile biocatalyst is discussed.

Application of nitrite converting enzymes for the production of useful compounds

Abstract

ChemInform Abstract: Microbial Biotransformations of Nitriles

ChemInformVolume 20, Issue 2 Reviews ChemInform Abstract: Microbial Biotransformations of Nitriles L. A. THOMPSON, L. A. THOMPSON Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorC. J. KNOWLES, C. J. KNOWLES Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorE. A. LINTON, E. A. LINTON Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorJ. M. WYATT, J. M. WYATT Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this author L. A. THOMPSON, L. A. THOMPSON Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorC. J. KNOWLES, C. J. KNOWLES Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorE. A. LINTON, E. A. LINTON Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this authorJ. M. WYATT, J. M. WYATT Microbial Biochem., Univ. Kent, Canterbury, Kent CT2 7NJ, UKSearch for more papers by this author First published: January 10, 1989 https://doi.org/10.1002/chin.198902333Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume20, Issue2January 10, 1989 RelatedInformation