A study based on more than 12,000 ammonites found along a one-hundred-meter-thick continuous pelagic section at La Charce (Drôme), together with comparisons with other west-European deep-water sections, allows to describe the very steps of the turnover affecting the Neocomitids, which spanned the depositional time of the late lowstand and transgressive systems tracts in the uppermost Trinodosum zone, before the maximum flooding in the Radiatus zone. We suggest that migrations in open marine environments as a response to climatic changes (from the warm lowstand to the cool transgression) were the main driving mechanism of the evolution of the fauna, a transformation which is rooted in the late lowstand but accelerated during the transgression. Many biostratigraphic horizons are individualized in the upper Valanginian.