Bioscience Laboratories Research Articles

Virilizing tumors of the adrenal cortex.

FUNCTIONING neoplasms of the adrenal cortex are clinically manifested by virilization, Cushing's syndrome, feminization, or hyperaldosteronism. In the present report, seven patients with virilizing adrenal tumors are described. In one case, virilization was present from birth. This is a unique instance of in utero virilization attributable to a tumor. Other unusual clinical, pathological, and laboratory findings are being reported. <h3>Methods</h3> The method used for 17-ketosteroid (17-KS) measurements was a modification of that of Callow et al.¹The procedure of Allen et al²was used for dehydroisoandrosterone (DHA). Pregnanetriol was measured at the BioScience Laboratories, Los Angeles, by Cox's method.³The 17-hydroxycorticosteroids (17-OHCS) were measured by a modification⁴of the method of Glenn and Nelson.⁵Aldosterone was estimated in patient 1 by a modification of the procedure of Neher and Wettstein.⁶Cathecholamines were measured by the method of Jacobs et al,⁷and vanillyl mandelic acid as described

Statistical Technics for Collaborative Tests. W. J. Youden, Association of Official Analytical Chemists, 1967, 60 pp

Journal Article Statistical Technics for Collaborative Tests. W. J. Youden, Association of Official Analytical Chemists, 1967, 60 pp Get access Richard J Henry, M.D. Richard J Henry, M.D. Bio-Science Laboratories Van Nuys, Calif. 91405 Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 14, Issue 1, 1 January 1968, Page 79, https://doi.org/10.1093/clinchem/14.1.79 Published: 01 January 1968

Experimental Epilepsy in Rabbits: Failure to Detect a Difference in Phenylalanine Metabolism in Convulsant Rabbits.

In the search for the biochemical abnormality which might be associated with the susceptibility to audiogenic seizures in strains of rabbits previously described (1,2), the conversion of phenylalanine to tyrosine by these rabbits was studied. This investigation was carried out because Coleman had reported greatly reduced in vivo production of tyrosine in 2 dilute strains of mice susceptible to audiogenic seizures (3).Materials and methods. All animals came from intensely inbred lines of seizure-susceptible and seizure-resistant rabbits. The L-phenylalanine was obtained from Mann Research Laboratories. In preliminary studies phenylalanine determinations were made by Bio-Science Laboratories; the later phenylalanine and tyrosine determinations were made at Children's Memorial Hospital(4). On the basis of the initial studies, it was hypothesized that phenylalanine metabolism in the rabbit related to coat pigmentation, but not to seizure-susceptibility.To test this hypothesis 6 nonpigmented and 6 pigmented co...

NEONATAL THYROIDAL HYPERACTIVITY. RESPONSE TO COOLING.

Introduction During the first few days of postnatal life, serum hormonal iodine values 1-3 and triiodothyronine I 131 erythrocyte uptake 4,5 have been shown to increase significantly. In a previous report we have shown that these increases occur in association with an early (10-24 hours of age) high rate of thyroid I 131 clearance 6 which suggests that this neonatal thyroidal hyperactivity may be the result of stimulation by thyroid stimulating hormone (TSH) during the early hours of postnatal life. The present studies report further investigations of thyroid radioiodine uptake and studies of the influence of neonatal cooling and exogenous adrenal cortical steroid on neonatal thyroidal hyperactivity. Methods Protein-bound iodine (PBI) determinations were conducted at Bioscience Laboratories by Barker's alkaline ashing technique. Fractional thyroid radioiodine clearance measurements ( k 1 )* and 24-hour thyroid I 131 uptake were conducted by methods previously reported. 6 Radioiodine doses of 1μC were employed in the

Simplified antistreptococcal deoxyribonuclease assay.

Olitzky, Irving (Bio-Science Laboratories, Los Angeles, Calif.), Sam Berkman, and Doris Bass. Simplified antistreptococcal deoxyribonuclease assay. J. Bacteriol. 84:1011-1015. 1962.-A simple antistreptococcal deoxyribonuclease assay has been devised in which dilutions of the patient's serum are reacted with deoxyribonuclease, and residual active enzyme is then detected by the Di Salvo technique using commercially available agar medium containing deoxyribonucleic acid. Use of this test as supplemental to antistreptolysin-O titrations leads to increased positive findings when searching for streptococcal antibodies.

Simplification of the spectrophotometric determination of glutamic-oxalacetic transaminase in blood serum.

Simplification of the Spectrophotometric Determination of Glutamic-Oxalacetic Transaminase in Blood Serum Get access Charles Sobel, B.S., Charles Sobel, B.S. Bio-Science Laboratories, Los Angeles, California Mr. Sobel is Chief Chemist, Dr. Berkman is Associate, and Mr. Swabb is Chemist. Search for other works by this author on: Oxford Academic Google Scholar Sam Berkman, Ph.D., Sam Berkman, Ph.D. Bio-Science Laboratories, Los Angeles, California Mr. Sobel is Chief Chemist, Dr. Berkman is Associate, and Mr. Swabb is Chemist. Search for other works by this author on: Oxford Academic Google Scholar Norman Swabb, M.S. Norman Swabb, M.S. Bio-Science Laboratories, Los Angeles, California Mr. Sobel is Chief Chemist, Dr. Berkman is Associate, and Mr. Swabb is Chemist. Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 26, Issue 12_ts, December 1956, Pages 1477–1478, https://doi.org/10.1093/ajcp/26.12_ts.1477 Published: 01 December 1956 Article history Received: 23 May 1956 Accepted: 31 July 1956 Published: 01 December 1956