The effects of alkaline treatment solution and concentration on the formation and adhesive strength of biomimetic synthesized bone-like apatite on Ti6Al4V surface were examined. Specimens were soaked in 5, 10 M solutions of NaOH or in 0.01, 0.03 M solutions of Ca(OH)2 at 80°C for 24 and 72 h. Subsequently, the substrates were heat-treated at 600°C for 1 h for consolidation of sodium or calcium titanate hydrogel layers. Finally, they were soaked in simulated body fluid (SBF) for one, three, and 14 days at 36.5°C. The surface of the specimens was characterized using scanning electron microscopy and thin film X-ray diffraction. It was found that the optimum condition for desirable apatite formation is 72 h soaking in 5 M NaOH at 80°C and three days’ immersion in SBF. The best adhesive strength result was for 24 h soaking in 0.03 M Ca(OH)2 at 80°C and 14 days’ immersion in SBF.