Biomedicine Industries Research Articles

Insights into dynamic evolution of glucuronofucogalactoglucan from water extract of Agrocybe cylindracea during maturation

This study explored the physicochemical properties and structural characteristics of Agrocybe cylindracea polysaccharides at four developmental stages, as well as their dynamic evolution during maturation. Results showed that the polysaccharides from A. cylindracea water extract exhibited similar structural characteristics across all four maturity stages, despite a significant reduction in yields. Four water-soluble heteroglycans, including one high molecular weight (ACPM-Et50-I) and three low molecular weight (ACPM-Et50-II, ACPM-Et60, ACPM-Et80), were isolated from A. cylindracea at each maturity stage. ACPM-Et50-I was identified as branched heterogalactans, while ACPM-Et60 and ACPM-Et80 were branched heteroglucans. However, ACPM-Et50-II was characterized as a branched glucuronofucogalactoglucan at the tide-turning stage but a glucuronofucoglucogalactan at the pileus expansion stage due to the increase of its α-(1 → 6)-D-Galp. In general, although the structural skeletons of most A. cylindracea heteroglycans were similar during maturation as shown by their highly consistent glycosyl linkages, there were still differences in the distribution of some heteroglucans. This work has for the first time reported a glucuronofucogalactoglucan in A. cylindracea and its dynamic evolution during maturation, which may facilitate the potential application of A. cylindracea in food and biomedicine industries.

Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7.

In the era of global climate change, the increasingly severe Fusarium head blight (FHB) and deoxynivalenol (DON) contamination have caused economic losses and brought food and feed safety concerns. Recently, an FHB resistance gene Fhb7 coding a glutathione-S transferase (GST) to degrade DON by opening the critical toxic epoxide moiety was identified and opened a new window for wheat breeding and DON detoxification. However, the poor stability of Fhb7 and the elusiveness of the catalytic mechanism hinder its practical application. Herein, we report the first structure of Fhb7 at 2.41 Å and reveal a unique catalytic mechanism of epoxide opening transformation in GST family proteins. Furthermore, variants V29P and M10 showed that 5.5-fold and 266.7-fold longer half-life time than wild-type, respectively, were identified. These variants offer broad substrate scope, and the engineered biosafe Bacillus subtilis overexpressing the variants shows excellent DON degradation performance, exhibiting potential at bacterium engineering to achieve DON detoxification in the feed and biomedicine industry. This work provides a profound mechanistic insight into the enzymatic activities of Fhb7 and paves the way for further utilizing Fhb7-related enzymes in crop breeding and DON detoxification by synthetic biology.

Discarding metal incorporation in pyrazole macrocycles and the role of the substrate on single-layer assemblies.

Pyrazole derivatives are key in crystal engineering and liquid crystal fields and thrive in agriculture, pharmaceutical, or biomedicine industries. Such versatility relies in their supramolecular bond adaptability when forming hydrogen bonds or metal-pyrazole complexes. Interestingly, the precise structure of pyrazole-based macrocycles forming widespread porous structures is still unsolved. We bring insight into such fundamental question by studying the self-assembled structures of a bis-pyrazole derivative sublimed in ultra-high-vacuum conditions (without solvents) onto the three (111) noble metal surfaces. By means of high-resolution scanning tunneling microscopy that is validated by gas phase density functional theory calculations, we find a common hexagonal nanoporous network condensed by triple hydrogen bonds at the molecule-metal interface. Such assembly is disrupted and divergent after annealing: (i) on copper, the molecular integrity is compromised leading to structural chaos, (ii) on silver, an incommensurate new oblique structure requiring molecular deprotonation is found and, (iii) on gold, metal-organic complexes are promoted yielding irregular chain structures. Our findings confirm the critical role of these metals on the different pyrazole nanoporous structure formation, discarding their preference for metal incorporation into the connecting nodes whenever there is no solvent involved.

Comparative physiological study of sea cucumbers from eastern waters of United States.

Sea cucumbers, belonging to the phylum Echinodermata, are known to possess valuable bioactive compounds that have medicinal properties. In several countries, such as Korea, China, and Japan, they are cultured in the aquaculture industries for food and medicinal purposes. Research has shown that different species of sea cucumbers each possesses unique medicinal values. As a result, we strive towards finding species with better health resilience in aquaculture system to be cultured for nutritional and medicinal purposes. In this paper, we compared the physiological and immunological parameters of three species of sea cucumbers, Cucumaria frondosa (C. frondosa), Isostychopus badionotus (I. badionotus), and Pentacta pygmaea (P. Pygmaea) from the waters of the eastern United States as they have not been studied extensively. Four different cells of sea cucumbers, phagocytic, red spherule, white spherule, and vibratile cells, that contribute to their immunity were counted. C. frondosa exhibited the highest concentrations of phagocytic cells, white spherule cells, and vibratile cells, compared to the two other species. Due to its high phagocytic cell concentration, the highest phagocytic capacity was seen in C. frondosa although it was not statistically significant. We also observed that C. frondosa had the highest total cell count and the highest concentration of coelomic protein among the three species. Lastly, C. frondosa possessed the highest lysozyme activity. Taken together, we concluded that C. frondosa is the best of the three species compared to be reared in the aquaculture systems for use in the food and biomedicine industries due to its immunological and physiological properties.

Molecular breeding of livestock for disease resistance

Animal infectious diseases pose a significant threat to the global agriculture and biomedicine industries, leading to significant economic losses and public health risks. The emergence and spread of viral infections such as African swine fever virus (ASFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and avian influenza virus (AIV) have highlighted the need for innovative approaches to develop resilient and disease-resistant animal populations. Gene editing technologies, such as CRISPR/Cas9, offer a promising avenue for generating animals with enhanced disease resistance. This review summarizes recent advances in molecular breeding strategies for generating disease-resistant animals, focusing on the development of disease-resistant livestock. We also highlight the potential applications of genome-wide CRISPR/Cas9 library screening and base editors in producing precise gene modified livestock for disease resistance in the future. Overall, gene editing technologies have the potential to revolutionize animal breeding and improve animal health and welfare.

Influences of Trypsin Pretreatment on the Structures, Composition, and Functional Characteristics of Skin Gelatin of Tilapia, Grass Carp, and Sea Perch.

Fish skin gelatin is an important functional product in the food, cosmetics, and biomedicine industries, and establishing a green and effective fish skin gelatin extraction method is an effective way to obtain high-quality gelatin and improve its production efficiency. In this study, a trypsin method was used to extract the skin gelatin of sea perch, tilapia, and grass carp, and the microstructures of skin gelatin of these three fish species were analyzed, with such functional characteristics as thermal stability, gel strength, and emulsifying properties measured. The study results show that the skin gelatin of sea perch and tilapia obtained through the trypsin method has a relatively big molecular mass, a dense network structure, and a stable trihelix conformation. In addition, the skin gelatin of these three fish species has a relatively high β-turn content in the secondary structure, good gel strength, and water absorption properties. The compositions of the collagen-associated proteins in the skin gelatins of these three fish species extracted with the trypsin method are significantly different from each other, with positive effects of decorin and biglycan on the stability of the network structure of gelatin and a certain damaging effect of metalloendopeptidase on the network structure of gelatin. The skin gelatin of tilapia has high thermal stability and good emulsifying performance. Therefore, this gelatin type has bright application prospects in such fields as food processing, cosmetics, and drug development. In contrast, the skin gelatin of grass carp has poor functional properties. Therefore, there are significant differences among the structures and functions of skin gelatin extracted from different kinds of fish through the trypsin method. This finding has provided a useful reference for the production of customized fish gelatin according to demand.

Comparison of two kinds of Agrocybe cylindracea polysaccharides: Structural characteristic and antitumor activity

Agrocybe cylindracea is one of the common sources of functional polysaccharides. But the structure and function of Agrocybe cylindracea polysaccharides obtained by different preparation methods are very different. A comprehensive and detailed study of its fine structure and systematic activity is helpful for its development and utilization. In this study, a cold water-souble polysaccharide (ACP) and an alcohol-soluble polysaccharide (ACAP) from Agrocybe cylindracea were extracted. Their physicochemical property, structural feature and antitumor activity in vitro were compared and investigated. The results showed that ACP (2840.9 kDa) was mainly composed of 1,6-linked-Glcp, 1,3,6-linked-Glcp, 1,6-linked-Galp and T-Glcp and inhibited MGC-803 cells proliferation via inducing tumor cells apoptosis by the cytochrome c/caspases pathway. However, ACAP (1823.9 Da) just contained glucose. By comparison, ACAP exhibited no inhibitory effect on MGC-803 cells in vitro. This work would provide an additional explanation for the structure-activity relationship of polysaccharides from Agrocybe cylindracea and provide a theoretical support for its application in biomedicine industries.

Dietary hydroxyproline promotes collagen deposition in swim bladder through regulating biosynthesis of amino acid: In-vitro and in-vivo investigations in Nibea coibor

Collagen, as a commercial and functional protein, is widely applied in food, comestic and biomedicine industries, which has a great market demand in recent years. Swim bladder is an ideal optional source for collagen production, due to its high collagen content and low risk of infectious pathogens. Previously, we found that dietary hydroxyproline (Hyp) could promote grow performance and collagen deposition in swim bladder of Nibea coibor, an economic Sciaenidae fish for production of high-grade swim bladder. However, little was known concerning the molecular mechanism underlying the effects of Hyp on collagen deposition. Here, we demonstrated that Hyp could up-regulate collagen I, prolyl hydroxylase (PH) contents and expression levels of genes related to collagen synthesis (col1a1, col1a2, p4ha1, etc.). RNA sequencing indicating that the “Biosynthesis of amino acids” pathway was significantly enriched by Hyp. Correspondingly, the amino acid profile of swim bladder cell was significantly affected by Hyp. Notably, the contents of major substrate amino acids for collagen synthesis (glycine, proline, etc.) were significantly increased, which may be partially related to the up-regulation of shmt gene involving in glycine biosynthesis. Consistent with the results in vitro, the role of Hyp in collagen I deposition, amino acid profile, expression of key regulatory genes in the enriched pathways were further verified in swim bladder of Nibea coibor. These results suggested that dietary Hyp could promote collagen deposition in swim bladder by enhancing collagen synthesis and regulating biosynthesis of amino acids (glycine, proline, etc.) in swim bladder. Our study would provide new insights into the regulatory mechanism underlying the effects of Hyp on collagen deposition in fish and lay foundation for developing nutritional regulation strategy to promote collagen production from aquatic species.

Preparation of Multiscale Slippery Liquid‐Infused Porous Surface Based on Ti6Al4V Alloy with Self‐Cleaning, Stability, and Self‐Healing Properties

Ti6Al4V alloy with excellent mechanical, physical, and chemical properties is now widely used in marine, aerospace, and biomedicine industry. Endowing Ti6Al4V alloy with better surface performances is of great significance and value. Herein, a convenient and commercial nanosecond laser etching method is utilized to fabricate a novel multiscale micro/nano porous structure based on Ti6Al4V alloy. Following the modification of the fluoroalkylsilane and the infusion of silicon oil, the slippery liquid‐infused porous surface (SLIPS) is successfully obtained. The wettability is studied in detail and indicates the SLIPS possesses excellent sliding property and hydrophobicity. The volume of the droplet and sample temperature are confirmed to have some significant impact on the sliding angles. Accordingly, a variety of liquids are able to slide down the tilted SLIPS (15.9°), indicating that the as‐prepared SLIPS demonstrates extraordinary liquid‐repellent ability. In addition, the multiscale micro‐/nanoporous structures can lock the silicon oil firmly and form a stable oil film on the surface, so the SLIPS also shows desirable self‐cleaning, self‐healing, and stability properties. Based on the study, it is promising that the SLIPS as‐prepared may expand the practical application of conventional Ti6Al4V alloy materials.

Brief Overview of Functionally Graded NiTi‐Based Shape Memory Alloys

Shape memory alloys (SMAs) are kinds of smart materials that have superior properties such as shape memory effect and superelasticity, which have the most potential applications in various fields, especially in aerospace, naval, automobile and biomedicine industries, etc. Nevertheless, the inherent natures of shape memory alloys are characterized by the smaller transformation temperature intervals and transformation stress intervals, which make the devices have poor control ability. To achieve the accurate controllability for progressive movement, creating functionally graded shape memory alloys has been adopted. Herein, the classification, fabrications, microstructural features, and performances of functionally graded shape memory alloys are reviewed. For comparison, the creation of various gradients in shape memory alloys can widen the transformation temperature intervals and transformation stress intervals to some extent. In addition, the formation of the compositional, microstructural, or geometrical gradient also contributes to the generation of excellent performances such as the four‐way shape memory effect, higher strength, and larger temperature window for the stress‐assisted two‐way shape memory effect, etc. Such superior functions promote a wider application range of shape memory alloys.

Effects of the Yaw Angle on Air Drag Reduction for Various Riblet Surfaces.

Biomimetic riblet surfaces, such as blade, wavy, sinusoidal, and herringbone riblet surfaces, have widespread applications for drag reduction in the energy, transportation, and biomedicine industries. The drag reduction ability of a blade riblet surface is sensitive to the yaw angle, which is the angle between the design direction of the riblet surface and the average flow direction. In practical applications, the average flow direction is often misaligned with the design direction of riblet surfaces with different morphologies and arrangements. However, previous studies have not reported on the drag reduction characteristics and regularities related to the yaw angle for surfaces with complex riblet microstructures. For the first time, we systematically investigated the aerodynamic drag reduction characteristics of blade, wavy, sinusoidal, and herringbone riblet surfaces affected by different yaw angles. A precisely adjustable yaw angle measurement method was proposed based on a closed air channel. Our results revealed the aerodynamic behavior regularities of various riblet surfaces as affected by yaw angles and Reynolds numbers. Riblet surfaces with optimal air drag reduction were obtained in yaw angles ranging from 0 to 60° and Reynolds numbers ranging from 4000 to 7000. To evaluate the effects of the yaw angle, we proposed a criterion based on the actual spanwise spacing (d+) of microstructure surfaces with the same phase in a near-wall airflow field. Finally, we established conceptual models of aerodynamic behaviors for different riblet surfaces in response to changes in the airflow direction. Our research lays a foundation for practical various riblet surface applications influenced by yaw angles to reduce air drag.

Research on the evolution of the Chinese urban biomedicine innovation network pattern: An analysis using multispatial scales.

This paper addresses the spatial pattern of urban biomedicine innovation networks by separately using four scales, i.e., the national scale, interregional scale, urban agglomeration scale, and provincial scale, on the basis of Chinese biomedicine patent data from the incoPat global patent database (GPD) (2001-2020) and using the method of social network analysis (SNA). Through the research, it is found that (1) on the national scale, the Chinese biomedicine innovation network becomes denser from west to the east as its complexity continuously increases. Its spatial structure takes the form of a radial network pattern with Beijing and Shanghai as its centers. The COVID-19 pandemic has not had an obvious negative impact on this network at present. (2) On the interregional scale, the strength of interregional network ties is greater than that of intraregional network ties. The eastern, central and western biomedicine innovation networks appear to be heterogeneous networks with regional central cities as the cores. (3) At the urban agglomeration scale, the strength of intraurban-agglomeration network ties is greater than that of interurban-agglomeration network ties. The three major urban agglomerations have formed radial spatial patterns with central cities as the hubs. (4) At the provincial scale, the intraprovincial networks have poor connectivity and low internal ties strength, which manifest as core-periphery structures with the provincial capitals as centers. Our research conclusion helps to clarify the current accumulation of technology and offer guidance for the development of China's biomedicine industry.

Non-Fourier pseudoplastic nanofluidic transport under the impact of momentum slip and thermal radiation

In this modern era of research, fluid mechanics and particularly nanofluids flowing on the stretching surfaces are considered to be occurring more often due to their faster heat transfer mechanism. Mostly, the theoretical research related to nanofluids including this one contributed in studying the performance and reaction of nanomaterials against different physical constraints so that they have wide applications in several developed engineering industries like, atomic receptacles, transportation, microchip technology as well as biomedicine industry and food industry. Several fluids permeating in industrial and biological processes are pseudo-plastics. Their non-Newtonian behavior is usually related to structural reorganizations of the fluid molecules due to their flow. Keeping this in view, this study is an effort to investigate two-dimensional steady incompressible Cattaneo–Christov heat flux model of Carreau–Yasuda nanofluid in the presence of thermal radiation and velocity slip. The governed model is presented through partial differential equations which are transformed into ordinary differential equations by using similarity transformation. The final system of equations are solved numerically and the results are expressed through graphs and tables for fluid’s velocity temperature, concentration and physical quantities like heat and mass flux. It is observed that skin friction as well as local heat and mass flux decline with velocity slip parameter and heat transfer rises but mass flux at surface declines through increment in radiation parameter.

Cordyceps guangdongensis lipid-lowering formula alleviates fat and lipid accumulation by modulating gut microbiota and short-chain fatty acids in high-fat diet mice.

Obesity has caused serious health and economic problems in the world. Cordyceps guangdongensis is a high-value macrofungus with broad application potential in the food and bio-medicine industry. This current study aimed to estimate the role of C. guangdongensis lipid-lowering compound formula (CGLC) in regulating fat and lipid accumulation, gut microbiota balance, short-chain fatty acid (SCFA) contents, and expression levels of genes involved in fat and lipid metabolism in high-fat diet (HFD) mice. The results showed that CGLC intervention markedly reduced body weights and fat accumulation in HFD mice, improved glucose tolerance and blood lipid levels, and decreased lipid droplet accumulation and fat vacuole levels in the liver. CGLC decreased the ratio of Firmicutes and Bacteroidetes and increased the relative abundances of Bacteroides (B. acidifaciens) and Bifidobacterium (B. pseudolongum). In addition, CGLC treatment significantly promoted the production of SCFAs and regulated the relative expression levels of genes involved in fat and lipid metabolism in liver. Association analysis showed that several species of Bacteroides and most of SCFAs were significantly associated with serum lipid indicators. These results suggested that CGLC is a novel candidate formulation for treating obesity and non-alcohol fatty liver by regulating gut microbiota, SCFAs, and genes involved in fat and lipid metabolism.

Deep red emission from Cs4PbI6/CsPbI3/ZnS heterostructure for enhanced stability and photoluminescence quantum yield

Inorganic halide perovskites have been regarded as fancy materials for the lighting and bio-medicine industry due to their wondrous photo-physical features. However, the instability concerns restrict their usage to some extent. Here, we demonstrated deep red emission from Cs4PbI6/CsPbI3/ZnS heterostructure by using a typical hot injection process. The as-obtained Cs4PbI6/CsPbI3/ZnS heterostructure carries deep red emission at 679 nm, with remarkably high photoluminescence quantum yield greater than 90%, longer carrier lifetime, and enhanced structural stability. The improved optical features and structural stability have been accredited to the complementary passivation of the halide vacancies and uncoordinated lead species of CsPbI3 by the 0D Cs4PbI6/ZnS and the formation of PbS even at a relatively low synthesizing temperature. Thus, this proposed heterostructure has the potential to be used in different displays and medical applications.

Emulsifying properties and bioavailability of clove essential oil Pickering emulsions stabilized by octadecylaminated carboxymethyl curdlan

In the present study, clove essential oil (CEO) Pickering emulsions were stabilized by octadecylamine-modified carboxymethyl curdlan (CMCD-ODA) at different pH values. The droplet size and negatively charged zeta potential of the CMCD-ODA emulsions decreased as the pH increased from 3.0 to 11.0. Rheology results indicated that the CMCD-ODA polymer/emulsion prepared at pH 5.0 showed higher apparent viscosity and viscoelasticity than other pH conditions, which might prevent droplets from flocculating. The Pickering emulsions obtained at pH 5.0 were spherical droplets with a uniform size distribution and a mean diameter of 9.54 μm, and they exhibited excellent stability during 28 days of storage. The morphological structures of the emulsions investigated by confocal laser scanning microscopy and scanning electron microscopy indicated that the CMCD-ODA Pickering emulsion obtained at pH 5.0 was stabilized by loading amphiphilic CMCD-ODA polymer around the spherical oil droplets and forming a weak gel network structure. The CEO-loaded CMCD-ODA emulsions had higher antioxidant capacity than free CEO after 28 days of storage at pH 5.0. Given the good emulsion stability, antioxidant activity, and great antibacterial effect, the CEO-loaded carboxymethyl curdlan Pickering emulsion has promising applications in food, cosmetic, and biomedicine industries.

Development of a first-class undergraduate major in bioengineering facing the emerging engineering direction of biomedicine

In the "Tutorial for outline of the healthy China 2030 plan", biomedicine was listed as a key planning and development area. Shanghai government also lists biomedicine as an emerging pillar industry. The rapid development of biomedicine industry put higher requirement for talents. Taking the idea of cross integration, mutually beneficial development, inheritance and innovation, the School of Biotechnology of East China University of Science and Technology organically integrates bioengineering and pharmaceutical majors to develop a new undergraduate engineering program of biomedicine, which specially reforms the talent training practice from the aspects of developing a "trinity teaching" standard system, a "three integration, three convergence" curriculum system, and a "three comprehensive education" innovative talent training system. We put forward the trinity of "value guidance, knowledge system, technology and non-technical core competence literacy" to foster emerging biomedicine engineering talents, and developed a comprehensive innovative talents training mode featured by "covering class-in and class-out, covering every student, and covering ideology and curriculum". Moreover, we established effective connections between courses and training goals, between general education courses and professional courses, and between top-notch talent training systems and training programs. Based on the achievements of teaching reform of the emerging engineering program "intelligent bio-manufacturing", the experience we obtained may provide ideas for development of the first-class bioengineering major in China.

Cloning, tissue distribution and mRNA expression of type I collagen alpha 1 gene from Chu's croaker (Nibea coibor)

The demand for collagen has been increasing over years due to its wide application in food, cosmetics and biomedicine industries. The synthesis of collagen protein in fish depends on instructions provided by collagen, type I, alpha 1 (COL1A1) gene. However, cloning, tissue distribution and mRNA expression of COL1A1 gene in a gel-producing Chu's croaker (Nibea coibor) is currently unknown. This study cloned the cDNA of COL1A1 gene (GenBank accession number: MK641512) from six N. coibor fish. The distribution and mRNA expression pattern of COL1A1 was analyzed in eight tissues of N. coibor. The COL1A1 cDNA had a full length of 6130 bp and contained a 4344 bp open reading frame (ORF) encoding a polypeptide of 1448 amino acids. The homology of N. coibor COL1A1 amino acid had 98% similarity with Larimichthys crocea, indicating conservatism with other members in same family (Sciaenidae). The deduced polypeptide contained the same signal peptides, C-propeptide and N-propeptide domains, and triple helix domains, which are the characteristics of type I collagen in vertebrates. The mRNA of COL1A1 gene was expressed significantly higher in the spine of N. coibor than in all other tissues (P < 0.05), followed by swim bladder, skin and scales. The swim bladder had higher collagen and hydroxyproline contents than other tissues, followed by spine >, scales > and > skin (P < 0.05). Our study successfully cloned the COL1A1 gene from N. coibor for the first time. The COL1A1 gene contained all the features of collagen pro-α1(I) chain proteins, and shared high homology with other marine teleost. COL1A1 gene in N. coibor is highly expressed in spine and swim bladder, consistent with collagen distribution. Our study contributes to better understanding on collagen biosynthesis in N. coibor tissues for various industrial uses.

Evaluation of biomedical industry technological innovation ability based on a grey panel clustering model.

Technological innovation in the biomedical industry is the basis for improving the core competitiveness of the biomedical industry and promoting the high-quality development of the industry. However, the technological innovation capacity of China's biomedical industry is not balanced, and there are great differences between regions. Therefore, accurately grasping the status quo of technological innovation in China's biomedical industry and assessing regional differences are of great significance and effect for the country to formulate targeted policies and systems. In view of this, this paper designs a two-stage biomedical industry technological innovation capability evaluation index system from the perspective of the innovation value chain. According to the panel data of China's biomedical industry from 2012 to 2018, a grey relational clustering model based on panel data is constructed and used to evaluate the technological innovation capability of China's biomedicine industry from two dimensions: the level of technological research and development and the ability to transform technological achievements.

Characterization and purification of β−secretase inhibitory peptides fraction from sea cucumber (Holothuria spinifera) enzymatic hydrolysates

Alzheimer’s disease (AD) is widely known as a progressive neurodegenerative disorder, mainly found in the elderly population. Progressive neurodegeneration in AD can lead to cognitive functional and behavioral alterations, thus affecting public health. Based on the amyloid cascade hypothesis, enzymatic cleavage of amyloid precursor protein (APP) by β-secretase and the formation of amyloid-beta (Aβ) peptides lead to neuron cell degradation. In this study, we focused on this hypothesis and investigated bioactivities of dried Holothuria spinifera enzymatic hydrolysates. Dried H. spinifera contained 79.77 % protein with glycine being the most abundant amino acid (178.89 g/kg). Trypsin hydrolysate of H. spinifera exhibited the highest β-secretase inhibitory activity with an IC50 value of 81.94 μg/mL. RP-HPLC produced an active fraction (HPLC-F3) containing four bioactive peptides (YPIEHGIVTNWDDM*EK, IEELEEEIEAER, EYVEETTGDEYVSLK, YPIEHGIVTNWDDMEK). The predominant peptide presented in HPLC-F3 was IEELEEEIEAER. Effects of HPLC-F3 against SH-SY5Y neuroblastoma cells were tested. HPLC-F3 reduced cellular levels of β-secretase enzyme, Aβ, and soluble amyloid precursor protein beta (sAPPβ) proteins. HPLC-F3 also protected SH-SY5Y cells from oxidative stress by decreasing phosphorylation of c-Jun N-terminal kinases and p38 mitogen-activated protein kinases. Results of this study suggest that H. spinifera is a potential source for functional foods and biomedicine industries.