Biomedical Applications Research Articles

Synthesis, Functionalization, and Biomedical Applications of Iron Oxide Nanoparticles (IONPs)

Iron oxide nanoparticles (IONPs) have garnered significant attention in biomedical applications due to their unique magnetic properties, biocompatibility, and versatility. This review comprehensively examines the synthesis methods, surface functionalization techniques, and diverse biomedical applications of IONPs. Various chemical and physical synthesis techniques, including coprecipitation, sol–gel processes, thermal decomposition, hydrothermal synthesis, and sonochemical routes, are discussed in detail, highlighting their advantages and limitations. Surface functionalization strategies, such as ligand exchange, encapsulation, and silanization, are explored to enhance the biocompatibility and functionality of IONPs. Special emphasis is placed on the role of IONPs in biosensing technologies, where their magnetic and optical properties enable significant advancements, including in surface-enhanced Raman scattering (SERS)-based biosensors, fluorescence biosensors, and field-effect transistor (FET) biosensors. The review explores how IONPs enhance sensitivity and selectivity in detecting biomolecules, demonstrating their potential for point-of-care diagnostics. Additionally, biomedical applications such as magnetic resonance imaging (MRI), targeted drug delivery, tissue engineering, and stem cell tracking are discussed. The challenges and future perspectives in the clinical translation of IONPs are also addressed, emphasizing the need for further research to optimize their properties and ensure safety and efficacy in medical applications. This review aims to provide a comprehensive understanding of the current state and future potential of IONPs in both biosensing and broader biomedical fields.

Alginate Formulation for Wound Healing Applications.

Significance: Alginate, sourced from seaweed, holds significant importance in industrial and biomedical domains due to its versatile properties. Its chemical composition, primarily comprising β-D-mannuronic acid and α-L-guluronic acid, governs its physical and biological attributes. This polysaccharide, extracted from brown algae and bacteria, offers diverse compositions impacting key factors such as molecular weight, flexibility, solubility, and stability. Recent Advances: Commercial extraction methods yield soluble sodium alginate essential for various biomedical applications. Extraction processes involve chemical treatments converting insoluble alginic acid salts into soluble forms. While biosynthesis pathways in bacteria and algae share similarities, differences in enzyme utilization and product characteristics are noted. Critical Issues: Despite its widespread applicability, challenges persist regarding alginate's stability, biodegradability, and bioactivity. Further understanding of its interactions in complex biological environments and the optimization of extraction and synthesis processes are imperative. Additionally, concerns regarding immune responses to alginate-based implants necessitate thorough investigation. Future Directions: Future research endeavors aim to enhance alginate's stability and bioactivity, facilitating its broader utilization in regenerative medicine and therapeutic interventions. Novel approaches focusing on tailored hydrogel formations, advanced drug delivery systems, and optimized cellular encapsulation techniques hold promise. Continued exploration of alginate's potential in tissue engineering and wound healing, alongside efforts to address critical issues, will drive advancements in biomedical applications.

Physical and biological characteristics of electrospun poly (vinyl alcohol) and reduced graphene oxide nanofibrous structure

The fabrication of graphene-based nanocomposites has been a topic of increasing interest due to graphene’s exceptional physical properties and the ability to enhance the properties of various polymeric materials. Evaluating the biocompatibility of these nanocomposites is crucial to ensure their safe and effective use in biomedical applications. This study characterized and assessed the biocompatibility of previously fabricated electrospun polyvinyl alcohol (PVA)/reduced graphene oxide rGO fibrous structures by conducting a comprehensive assessment of their physical and biological characteristics. Contact angle measurements revealed that adding rGO to electrospun PVA fibers enhanced the surface wettability, improving the fibrous structure’s PBS absorption capacity and degradation behavior. Including the rGO content resulted in a higher water vapor transmission rate, reaching ∼48 g/m2·day for PVA + 0.5 wt.% rGO and ∼45 g/m2·day for PVA + 1.0 wt.% rGO, compared to ∼40 g/m2·day for electrospun PVA fibers. Cell culture studies, including MTT assay, alkaline phosphatase (ALP) activity analysis, alizarin red staining, fluorescence microscopy, and SEM analyses, demonstrated that electrospun PVA + 1.0 wt.% rGO nanocomposites exhibited superior cell viability, proliferation, and growth compared to other samples, due to the improved physical properties of the PVA + 1.0 wt.% rGO fibrous structure.

Evaluation of surface properties of modified Ti6Al4V alloy with copper nanoparticles organic nanostructure for biomedical applications: dependency on anticorrosive, antibacterial, and biocompatibility

Abstract In this study, a novel multifunctional copper nanoparticle CuNPs in the organic biomatrix was coated to the surface of Ti6Al4V to create multifunctional features. The synthesis of CuNPs was carried out by plant-mediated green synthesis method obtained from Moringa leaf extract, and the prepared CuNPs were coated on the substrate surfaces as single and double layers with drop casting methods. Characterizations of the synthesized CuNPs were performed by UV–Vis, FTIR, XRD, and SEM methods. Characterization of the modified Ti6Al4V alloy surfaces was performed using SEM-EDS and surface roughness analysis. The electrochemical corrosion, antibacterial behavior, and cytotoxic effects of coated and noncoated Ti6Al4V as a function of biocompatibility properties were also tested. The synthesized CuNPs have a homogeneously dispersed spherical shape. Biocorrosion tests have clearly demonstrated that the coating forms a protective film on the substrate surface, and the resistance increased by 49 %. Antibacterial results show that the single and double-coated Ti6Al4V alloy samples with CuNPs organic nanostructure had improved biocompatibility. However, it was determined that the cytotoxic effect increases proportionally with the coating. The obtained results show the importance of surface modification in the appropriate nanostructure to obtain multifunctional nanoplatforms that show promise in biomedical applications.

Development of Alginate Hydrogels Incorporating Essential Oils Loaded in Chitosan Nanoparticles for Biomedical Applications

A hybrid alginate hydrogel–chitosan nanoparticle system suitable for biomedical applications was prepared. Chitosan (CS) was used as a matrix for the encapsulation of lavender (Lavandula angustifolia) essential oil (LEO) and Mentha (Mentha arvensis) essential oil (MEO). An aqueous solution of an acidic Natural Deep Eutectic Solvent (NADES), namely choline chloride/ascorbic acid in a 2:1 molar ratio, was used to achieve the acidic environment for the dissolution of chitosan and also played the role of the ionic gelator for the preparation of the chitosan nanoparticles (CS-NPs). The hydrodynamic diameter of the CS-MEO NPs was 130.7 nm, and the size of the CS-LEO NPs was 143.4 nm (as determined using Nanoparticle Tracking Analysis). The CS-NPs were incorporated into alginate hydrogels crosslinked with CaCl2. The hydrogels showed significant water retention capacity (>80%) even after the swollen sample was kept in the aqueous HCl solution (pH 1.2) for 4 h, indicating a good stability of the network. The hydrogels were tested (a) for their ability to absorb dietary lipids and (b) for their antimicrobial activity against Gram-positive and Gram-negative foodborne pathogens. The antimicrobial activity of the hybrid hydrogels was comparable to that of the widely used food preservative sodium benzoate 5% w/v.

Principles and Biomedical Applications of Self-Assembled Peptides: Potential Treatment of Type 2 Diabetes Mellitus

Type 2 diabetes mellitus (T2DM) is the most prevalent metabolic disorder worldwide. There have been tremendous efforts to find a safe and prolonged effective therapy for its treatment. Peptide hormones, from certain organisms in the human body, as the pharmaceutical agents, have shown outstanding profiles of efficacy and safety in plasma glucose regulation. Their therapeutic promises have undergone intensive investigations via examining their physicochemical and pharmacokinetic properties. Their major drawback is their short half-life in vivo. To address this challenge, researchers have recently started to apply the state-of-the-art molecular self-assembly on peptide hormones to form nanofibrillar structures, as a smart nanotherapeutic drug delivery technique, to tremendously enhance their prolonged bioactivity in vivo. This revolutionary therapeutic approach would significantly improve patient compliance. First, this review provides a comprehensive summary on the pathophysiology of T2DM, various efforts to treat this chronic disorder, and the limitations and drawbacks of these treatment approaches. Next, this review lays out detailed insights on various aspects of peptide self-assembly: adverse effects, potential applications in nanobiotechnology, thermodynamics and kinetics of the process, as well as the molecular structures of the self-assembled configurations. Furthermore, this review elucidates the recent efforts on applying reversible human-derived peptide self-assembly to generate highly organized smart nanostructured drug formulations known as nanofibrils to regulate and prolong the bioactivity of the human gut hormone peptides in vivo to treat T2DM. Finally, this review highlights the future research directions to advance the knowledge on the state-of-the-art peptide self-assembly process to apply the revolutionary smart nanotherapeutics for treatment of chronic disorders such as T2DM with highly improved patient compliance.

Enhancing genomic disorder prediction through Feynman Concordance and Interpolated Nearest Centroid techniques.

Clinical biomedical applications of genomic technologies are extensive and provide possibilities to enhance healthcare covering the span of medical talents. Genome disorder prediction is an important issue in biomedical research. Genome disorders cause multivariate diseases such as cancer, dementia, diabetes, Leigh syndrome, etc. Existing machine and deep learning-based methods were introduced to forecast genome disorders. However, the genome prediction outcomes were not sufficient. To address this issue, propose a new method called Quadratic Feynman Polynomial Interpolated and Vector Nearest Centroid-based (QFPI-VNC) for acutely predicting the genome disorder with improved sensitivity and specificity. First, we utilized medical data about children from a public genomes dataset and applied it to Linear Quadratic and Feynman Kac Genome filtering to obtain computationally efficient filtered results. Next, the results are fed to the Concordance Correlated Polynomial Interpolation with the purpose of extracting genome wide data in an accurate manner. Finally, the features extracted are fused and fed to the Support Vector and Nearest Centroid model for genome disorder prediction. Experimental investigations of the proposed method employing the genome dataset confirm that the performance of the proposed method is prospective and in the scope of acceptance with relative to state-of-the-art methods in terms of convergence speed, recognition rate, sensitivity, and specificity. Results suggest that the QFPI-VNC method produces the best performance with a higher genome disease detection rate by 14%, accuracy by 11%, sensitivity by 14% specificity by 12%, and lesser convergence speed by 29% than compared to state-of-the-art methods.

Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable Scaffolds.

Melt electrowriting (MEW) enables precise scaffold fabrication for biomedical applications. With a limited number of processable materials with short and tunable degradation times, polyhydroxyalkanoates (PHAs) present an interesting option. Here, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and a blend of PHBV and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (PHBV+P34HB) are successfully melt electrowritten into scaffolds with various architectures. PHBV+P34HB exhibits greater thermal stability, making it a superior printing material compared to PHBV in MEW. The PHBV+P34HB scaffolds subjected to enzymatic degradation show tunable degradation times, governed by enzyme dilution, incubation time, and scaffold surface area. PHBV+P34HB scaffolds seeded with human dermal fibroblasts (HDFs), demonstrate enhanced cell adherence, proliferation, and spreading. The HDFs, when exposed to the enzyme solutions and enzymatic degradation residues, show good viability and proliferation rates. Additionally, HDFs grown on enzymatically pre-incubated scaffolds do not show any difference in behavior compared those grown on control scaffolds. It is concluded that PHAs, as biobased materials with enzymatically tunable degradability rates, are an important addition to the already limited set of materials available for MEW technology.

Formulation, E-Beam Crosslinking, and Comprehensive Characterisation of Lavender Oil-Enriched Hydrogels

This study focused on the formulation, electron beam (e-beam) crosslinking, and characterisation of hydrogels enriched with lavender oil (LO) to enhance their structural and functional properties for biomedical applications. Stable hydrogels were synthesised using water-soluble polymers and suitable ratios of Tween 80 and Isopropyl alcohol (IPA) as surfactant and co-surfactant, respectively, via e-beam irradiation at doses up to 70 kGy. The most effective crosslinking was achieved with a radiation dose of 30 kGy, depending on the concentrations of surfactants and LO. LO-enriched hydrogels exhibited enhanced superabsorbent swelling (7700% to 18,000%) and faster equilibrium rates than the control hydrogel. Structural analysis revealed a flexible spongiform porous architecture with larger mesh sizes (156 nm to 246 nm) and adequate elastic moduli (130 to 308 Pa). Degradation tests aligned with swelling data, demonstrating a degradation rate of 12% after 35 days, indicating an appropriate balance of stability and degradation. These findings suggest that e-beam technology, in conjunction with LO and surfactant addition, can effectively tailor hydrogel properties for biomedical applications, making them promising candidates for further research in wound care, drug delivery systems, and other biological applications.

Green Hydrothermal Synthesis of Gallic Acid Carbon Dots: Characterization and Cytotoxic Effects on Colorectal Cancer Cell Line.

Colorectal cancer remains a leading cause of cancer-related deaths worldwide, necessitating the development of novel therapeutic approaches. Carbon dots (CDs) have emerged as promising nanoparticles for biomedical applications due to their unique properties. Gallic acid (GA), a known anticancer agent, is effective against various tumor types. This study explores the potential of gallic acid-derived carbon dots (GA-CDs) as an innovative anticancer agent against HCT-116 colorectal cancer cells, focusing on apoptosis signaling pathways. GA-CDs were synthesized using a one-pot hydrothermal method. Characterization was conducted using transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) absorption spectroscopy. The cytotoxicity of GA and GA-CDs on HCT-116 cells was evaluated using the MTT assay at various concentrations over 24 and 48 hours. Cellular uptake was assessed via fluorescence microscopy, and apoptosis was analyzed using acridine orange/propidium iodide (AO/PI) staining. Total RNA extraction followed by complementary DNA (cDNA) synthesis via RT-PCR was performed, and real time-PCR (Q-PCR) was conducted to examine the expression of apoptosis-related genes Caspase-3, Bax, and Bcl-2. Characterization confirmed the successful synthesis of spherical GA-CDs. GA-CDs exhibited dose- and time-dependent cytotoxicity, with IC50 values of 88.55 µg/mL for GA-CDs and 192.2 µg/mL for GA after 24 hours. Fluorescence microscopy confirmed the efficient uptake of GA-CDs by HCT-116 cells. AO/PI staining showed a significant increase in apoptotic cell numbers after treatment with GA-CDs. Q-PCR analysis revealed overexpression of Caspase-3 and Bax genes in GA-CD-treated cells, though no significant changes were observed in the expression of Bcl-2 or the Bax/Bcl-2 ratio. GA-CDs demonstrated potent anticancer properties by inducing apoptosis and reducing cell viability in HCT-116 cells. These findings suggest the potential of GA-CDs as a novel therapeutic agent for colorectal cancer treatment, warranting further investigation into their mechanism of action and in vivo efficacy.

Coordination-Driven in Situ Grown Copper Peroxide in Mesoporous Dopamine with Self-Supplied H2O2 for Synergistic Enhanced PTT/CDT Antibacterial Treatment and Wound Healing.

As antibiotic resistance increases, alternative antimicrobial methods become essential. Chemical dynamics therapy (CDT) utilizing copper peroxide (CuO2) nanodots shows significant potential in antibacterial applications due to its ability to self-supply hydrogen peroxide (H2O2) on its own. This characteristic effectively addresses the challenges of low H2O2 levels and high glutathione (GSH) expression in the bacterial infection microenvironment. However, its tendency to aggregate and instability greatly affect its effectiveness. Therefore, this study developed a coordination-driven strategy to prepare copper peroxide-loaded mesoporous polydopamine nanomaterials (CuO2@MPDA) through in situ growth of CuO2 in mesoporous polydopamine utilizing the chelating interaction between amino and catechol structures of MPDA with copper ions. This strategy not only ensures that copper peroxide is evenly distributed within the pores of mesoporous polydopamine but also protects it through the shielding effect of pores, greatly enhancing its dispersibility and stability. More notably, the loading of CuO2 enhances the photothermal performance of MPDA by broadening its light absorption range, and MPDA-mediated photothermal therapy (PTT) can accelerate CuO2 to produce more hydroxyl radicals by speeding up chemical reactions, resulting in a combined boost in PTT and CDT. The developed CuO2@MPDA nanomaterials at very low concentrations exhibit improved antibacterial efficiency both in vitro and in vivo. Overall, this study provides an innovative strategy to construct an antibacterial nanoplatform for synergistically enhanced PTT/CDT dual-mode antibacterial treatment, exhibiting great potential for future biomedical applications.

Biotechnological and Biomedical Applications of Enzymes Involved in the Synthesis of Nucleosides and Nucleotides—2nd Edition

The study of nucleic acid derivatives and their role in cellular processes, first addressed in our previous Special Issue (https://www [...]

Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications.

Biosurfactants, naturally produced by plants and microorganisms, closely mimic synthetic surfactants in physiochemical properties, making them valuable alternatives in various applications. They serve as antimicrobial agents and play a crucial role in immune regulations. These compounds find wide use in industries like food processing, biodegradation, pharmaceuticals, and naturally present in the skin, brain, lungs, and gut, maintaining membrane permeability for organ health. This review outlines the basic characteristics and classes of biosurfactants (glycolipids, lipopeptides, phospholipids, and glycoproteins) and explores their biomedical importance, emphasizing their anti-adhesive, antimicrobial, and immune-modulating properties. This review aimed to provide outline the fundamental characteristics of biosurfactants and deliver a brief overview of their different classes, including glycolipids, lipopeptides, phospholipids, and glycoproteins. Furthermore, this review also explore their biomedical significance, highlighting their anti-adhesive, antimicrobial, and immune-modulating properties.

Synthesis of pyrrole-fused dibenzoxazepine/dibenzothiazepine/triazolobenzodiazepine derivatives via isocyanide-based multicomponent reactions

An efficient and facile synthesis of pyrrole-fused dibenzoxazepine/dibenzothiazepine/triazolobenzodiazepine derivatives was developed through the isocyanide-based multicomponent reaction of isocyanides, gem-diactivated olefins, and cyclic imines such as dibenzoxazepine, dibenzothiazepine, and triazolobenzodiazepine under solvent- and catalyst-free conditions. Purposefully, this approach produced various bioactive scaffolds using environmentally friendly, mild, and simple conditions. Due to their bioactive moieties, these compounds with exclusive fluorescence properties may attract great attention in biomedical applications, clinical diagnostics, and conjugate materials.

Highly sensitive quad-ray shaped polarization insensitive THz meta-biosensor

Abstract Metasurface-based sensors are now becoming crucial for label-free and rapid-detection technologies in biomedical applications, leading to a growing demand for new highly sensitive meta-biosensors. This paper demonstrates a perfectly symmetrical quad-ray X-shaped THz meta-absorber (QRXMA), enabling narrowband and polarization insensitivity performance. The theoretical modelling and performance investigation focuses on its potential for improved refractive index (RI) sensing for multiple biological samples. The simulation outcomes reveal that the proposed optimal QRXMA achieves an absorption rate of 99.98% at 1.3 THz for both TE and TM polarized incidences. Furthermore, we highlight the adjustability of absorptive characteristics of the proposed device by altering the different geometric parameters. These findings underscore the QRXMA potential in RI sensing applications, achieving a sensitivity of approximately 116.8 GHz RIU−1 and a figure of Merit (FoM) of about 2 with an 8 μm thick non-destructive analyte layer. This research makes a significant contribution to the development of highly efficient meta-biosensors with promising applications in THz detection, sensing, and imaging.

Molecular Dynamics Simulations of Binary Phosphate Glass Using the ReaxFF Potential.

Due to the importance of the understanding of dissolution behavior of phosphate-based bioglasses (PBGs) in different biomedical applications, binary sodium and calcium phosphate glasses have been simulated for the first time using a newly developed ReaxFF force field and a standard melt-quench method with the LAMMPS classical molecular dynamics software. The partial radial distribution function of P-O within the first coordination shell indicated two distinct peaks corresponding to phosphorus bonding to NBO and BO, respectively, at distances consistent with those observed experimentally and a P-O coordination number of 4.0. Angular distribution functions were consistent with the experimental data. The calculated network connectivities are in good agreement with experimental data, and the detailed Qn distributions are broader than would be expected.

MHD Casson Fluid Flow over a Stretching Surface with Suction, Double Stratification, and Heat and Mass Transfer Effects

Abstract In this article, we investigated the flow of a magnetohydrodynamic Casson fluid across an extending surface with exponential permeability in a double-stratified medium. Thermal radiation, suction, heat sources, viscous dissipation, and chemical reactions drive the stream field. We converted the flow describing partial differential equations into terms of ordinary differential equations by applying appropriate transformations. Next, we utilised the bvp4c package in Matlab to obtain numerical solutions for these equations. We explored and graphically showed the implications of different non-dimensional governing parameters for temperature, concentration, and velocity profiles. After analysis, we provide a tabular presentation of the friction factor, Nusselt, and Sherwood numbers. The Casson fluid temperature shows a rising trend for the solutal stratification parameter and a decreasing trend for the thermal stratification parameter, while the Casson fluid velocity shows a declining trend towards both of these parameters. The Casson fluid concentration also behaves differently depending on the stratification parameter; for example, it increases for thermal stratification and decreases for solutal stratification. We notice that an increase in the Casson parameter's value suppresses the velocity field. However, as the Casson parameter increases, both the temperature and the concentration improve. Furthermore, comparisons with previously published findings also support the current results. \sethlcolor{yellow}\hl{The study of MHD Casson fluid flow that includes suction, dual stratification, and the effects of heat and mass transfer is very useful in many areas, such as polymer processing, metallurgical engineering, biomedical applications, environmental sciences, and advanced cooling technologies.

Wear and Corrosion Resistance of ZrN Coatings Deposited on Ti6Al4V Alloy for Biomedical Applications

Zirconium nitrides films were synthesized on Ti6Al4V substrates at a bias voltage of −50 V, −80 V, −110 V and −150 V by the direct current (DC) reactive magnetron sputtering technique. The as-deposited coatings were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). The wear and corrosion resistance of the obtained ZrN coatings were evaluated to determine the possibility for their implementation in modern biomedical applications. It was found that the intensity of the diffraction peak of the Zr-N phase corresponding to the (1 1 1) crystallographic plane rose as the bias voltage increased, while the ZrN coatings’ thickness reduced from 1.21 µm to 250 nm. The ZrN films’ surface roughness rose up to 75 nm at −150 V. Wear tests showed an increase in the wear rate and wear intensity as the bias voltage increased. Corrosion studies of the ZrN coatings were carried out by three electrochemical methods: open circuit potential (OCP), cyclic voltammetry (polarization measurements) and electrochemical impedance spectroscopy (EIS). All electrochemical measurements confirmed that the highest protection to corrosion is the ZrN coating, which was deposited on the Ti6Al4V substrate at a bias voltage of −150 V.

Inorganic-Based Nanoparticles and Biomaterials as Biocompatible Scaffolds for Regenerative Medicine and Tissue Engineering: Current Advances and Trends of Development

Regenerative medicine amalgamates stem cell technology and tissue engineering strategies to replace tissues and organs damaged by injury, aging, ailment, and/or chronic conditions by leveraging the innate self-healing mechanism of the body. The term ‘regenerative medicine’ was coined by William A. Haseltine during a 1999 conference on Lake Como. Since its inception in 1968, the field has offered clinical benefits for the regeneration, repair, and restoration of bones, skin, cartilage, neural tissue, and the heart, as well as scaffold fabrication. The field of tissue engineering and regenerative medicine can vastly benefit from advancements in nanoscience and technology, particularly in the fabrication and application of inorganic-based nanoparticles and bionanomaterials. Due to the tunable intrinsic properties, i.e., size, topography, surface charge, and chemical stability, inorganic-based nanoparticles and biomaterials have surpassed traditional synthetic materials. Given the wide gamut of near-future applications of inorganic nanoparticles and biomaterials, this article gives an overview of the emerging roles in stem cell regenerative research, tissue engineering, artificial skin and cartilage regeneration, neural nerve injuries, 3D bioprinting, and development of new inorganic bio-scaffolds. The review also addresses the challenges related to the clinical application and tissue compatibility of inorganic nanoparticles and biomaterials, utilizing current state-of-the-art techniques.

Tumor Cell-Specific Signal Processing Platform Controlled by ATP for Non-invasive Modulation of Cellular Behavior.

Regulating the spatial distribution of membrane receptors can artificially reprogram cellular behaviors, which play a critical biological role in various physiological and pathological processes. Herein, we construct a tumor cell-specific signal processing platform (TCS-SPP) for controlled promotion/inhibition of cellular-mesenchymal epithelial transition factor (c-Met) receptor dimerization to noninvasively modulate cellular behaviors. Upon the dual-aptamer recognition in the upstream input signal circuit (UISC) to discriminate target cancer cells, the membrane-anchored DNA signal processor (DSP) is activated for signal amplification via rolling circle amplification (RCA) followed by the working of an ATP molecular switch for signal conversion, achieving receptor modulation in the downstream output signal circuit (DOSC). Benefiting from the rigid structure of DSP, the protective effect, and spatial confinement effect of RCA products, this TCS-SPP has demonstrated good performance in accurately modulating cellular behavior such as cell migration, invasion, and proliferation, showing great potential for targeted cancer therapy and biomedical engineering applications.