Biomass Responses Research Articles

Differential responses of soil microbial biomass, diversity and interactions to land use intensity at a territorial scale

Impact of land use intensification on soil microbial communities across a territory remains poorly documented. Yet, it has to be deciphered to validate the results obtained at local and global scales by integrating the variations of environmental conditions and agricultural systems at a territorial scale. We investigated the impact of different land uses (from forest to agricultural systems) and associated soil management practices on soil molecular microbial biomass and diversity across a territory of 3300 km2 in Burgundy (France). Microbial biomass and diversity were determined by quantifying and high-throughput sequencing of soil DNA from 300 soils, respectively. Geostatistics were applied to map the soil macro-ecological patterns and variance partitioning analysis was used to rank the influence of soil physicochemical characteristics, land uses and associated practices on soil microbial communities. Geographical patterns differed between microbial biomass and diversity, emphasizing that distinct environmental drivers shaped these parameters. Soil microbial biomass was mainly driven by the soil organic carbon content and was significantly altered by agricultural land uses, with a loss of about 71 % from natural to agricultural ecosystems. The best predictors of bacterial and fungal richness were soil texture and pH, respectively. Microbial diversity was less sensitive than microbial biomass to land use intensification, and fungal richness appeared more impacted than bacteria. Co-occurrence network analysis of the interactions among microbial communities showed a decline of about 95 % of network complexity with land use intensification, which counterbalanced the weak response of microbial diversity. Grouping of the 147 cropland plots in four clusters according to their agricultural practices confirmed that microbial parameters exhibited different responses to soil management intensification, especially soil tillage and crop protection. Our results altogether allow evaluating the different levels of microbial parameters' vulnerability to land use intensity at a territorial scale.

A Lagrangian model for drifting ecosystems reveals heterogeneity-driven enhancement of marine plankton blooms

Marine plankton play a crucial role in carbon storage, global climate, and ecosystem function. Planktonic ecosystems are embedded in patches of water that are continuously moving, stretching, and diluting. These processes drive inhomegeneities on a range of scales, with implications for the integrated ecosystem properties, but are hard to characterize. We present a theoretical framework that accounts for all these aspects; tracking the water patch hosting a drifting ecosystem along with its physical, environmental, and biochemical features. The theory resolves patch dilution and internal physical mixing as a function of oceanic strain and diffusion. Ecological dynamics are parameterized by an idealized nutrient and phytoplankton population and we specifically capture the time evolution of the biochemical spatial variances to represent within-patch heterogeneity. We find that, depending only on the physical processes to which the water patch is subjected, the plankton biomass response to a resource perturbation can vary in size up to six times. This work indicates that we must account for these processes when interpreting and modeling marine ecosystems and provides a framework with which to do so.

Filter-feeding gelatinous macrozooplankton response to climate change and implications for benthic food supply and global carbon cycle.

It is often suggested that gelatinous zooplankton may benefit from anthropogenic pressures of all kinds and in particular from climate change. Large pelagic tunicates, for example, are likely to be favored over other types of macrozooplankton due to their filter-feeding mode, which gives them access to small preys thought to be less affected by climate change than larger preys. In this study, we provide model-based estimate of potential community changes in macrozooplankton composition and estimate for the first time their effects on benthic food supply and on the ocean carbon cycle under two 21st-century climate-change scenarios. Forced with output from an Earth System Model climate projections, our ocean biogeochemical model simulates a large reduction in macrozooplankton biomass in response to anthropogenic climate change, but shows that gelatinous macrozooplankton are less affected than nongelatinous macrozooplankton, with global biomass declines estimated at -2.8% and -3.5%, respectively, for every 1°C of warming. The inclusion of gelatinous macrozooplankon in our ocean biogeochemical model has a limited effect on anthropogenic carbon uptake in the 21st century, but impacts the projected decline in particulate organic matter fluxes in the deep ocean. In subtropical oligotrophic gyres, where gelatinous zooplankton dominate macrozooplankton, the decline in the amount of organic matter reaching the seafloor is reduced by a factor of 2 when gelatinous macrozooplankton are considered (-17.5% vs. -29.7% when gelatinous macrozooplankton are not considered, all for 2100 under RCP8.5). The shift to gelatinous macrozooplankton in the future ocean therefore buffers the decline in deep carbon fluxes and should be taken into account when assessing potential changes in deep carbon storage and the risks that deep ecosystems may face when confronted with a decline in their food source.

Widely naturalized species are not more promiscuous to different nitrogen forms, but benefit more from inorganic nitrogen

Nitrogen (N) has been considered a crucial factor influencing plant invasions. Many studies have assessed responses of alien plants to different N availabilities. However, in natural soils, N comes in different inorganic and organic forms. Few studies have explored yet whether responses of alien species to different N forms are related to their naturalization success globally. We selected 22 common herbaceous species native to Germany that have all become naturalized elsewhere in the world, but vary in their naturalization extent. We grew the species under six N conditions that differed in the availability or form of N, and assessed their growth performance. We found that neither biomass production nor promiscuity to different N forms was related to naturalization success of the species. However, the biomass response to inorganic N, relative to organic N, was stronger for the widely naturalized species than for the less widely naturalized ones. Our comparative multi-species source-area study shows that although the widely naturalized species were not more promiscuous than the less widely naturalized species, they took more advantage of the inorganic-N forms. This indicates that naturalization success might be partly driven by a species’ ability to take advantage of increased inorganic N levels.

Impacts of Atlantic water intrusion on interannual variability of the phytoplankton community structure in the summer season of Kongsfjorden, Svalbard under rapid Arctic change

Atlantification, known as impacts of high-latitude Atlantic water inflows on the Arctic Ocean has strengthened owing to climate change, corresponding to the rapid ice retreat in the Arctic. The relationship between phytoplankton and environmental changes in the Arctic on the interannual scale is unclear because of the lack of long-time series data. In this study, we discuss the ecological response to Atlantic water intrusion in the Kongsfjorden,Svalbard. We measured chlorophyll a and photosynthesis pigments for the water column samples from a fixed section along the Kongsfjorden to study the response of phytoplankton biomass and communities to Atlantic water intrusion in the summer season from 2007 to 2018. The results showed that dinoflagellates, prasinophytes, cryptophytes, and chlorophytes consistently accounted for over 50% of the total biomass, with the distinct annual variation of chlorophyll a. Bioavailable nitrogen was the main limiting factor on phytoplankton growth in the study area, as inferred by its concentration and nutrients ratios. The relationship between phytoplankton and water mass analysis suggested that the intrusion of Atlantic water in Kongsfjorden may cause interannual variability of the phytoplankton biomass and community structure by influencing the nutrient supply and water stratification in the fjord region. Our study provides insights into the ongoing impact of Atlantification on the phytoplankton community in the Arctic fjord.

Crucial roles of the optimal time-scale of water condition on grassland biomass estimation on Qinghai–Tibet Plateau

The effect of the time-scale of water conditions on vegetation productivity has been widely studied by the academic community. However, the relationship between the time-scale of water conditions and the vegetation growth rhythm and the effect of this relationship on vegetation biomass estimation have rarely been discussed. Here, we analyzed the occurrence times of major phenological events on alpine grasslands using the widely distributed “site-dominant species” dataset and set a series of time-scales for accumulated precipitation and standardized precipitation evapotranspiration index based on phenological information. Then, we combined large-scale aboveground/belowground biomass datasets to evaluate the role of the optimal time-scale for water conditions in aboveground/belowground biomass estimation. The results showed that (1) the optimal time-scale for water conditions with the greatest effects on aboveground biomass was on the month before the end of flowering or the onset of fruit maturity. The optimal time-scale for water condition effects on belowground biomass was earlier and longer than that for the aboveground biomass. The optimal time-scales for accumulated precipitation and standardized precipitation evapotranspiration index effects on belowground biomass were at five months before the end of flowering or the beginning of fruit ripening and the three months before the first flowering, respectively. (2) The aboveground and belowground biomass were underestimated by 11 % and 9 %, respectively, when the water conditions at the optimal time-scales were ignored. (3) The interannual variability in aboveground/belowground biomass was more effectively captured by considering the optimal time-scales of water conditions, especially in water-restricted areas. Overall, this study indicated that terrestrial carbon cycle models should incorporate information on the lag-effects of the water conditions in previous periods. In the future, increasing the number of belowground biomass observations and conducting monthly belowground biomass monitoring sooner will be key to revealing the mechanisms of the belowground biomass response to climate change.

Identification of Solanum pimpinellifolium genome regions for increased resilience to nitrogen deficiency in cultivated tomato

High-quality crop production with minimal fertilizer inputs is a key goal for the agriculture of the future. Globally, tomato is one of the most important vegetable crops and its intensive production and breeding has been based on the application of large quantities of nitrogen (N) fertilizers. Therefore, the development of N use efficient (NUE) cultivars with low N inputs needs to be addressed. Some variability in plant growth, fruit quality and NUE traits among tomato (Solanum lycopersicum L.) varieties under low N supply has been reported, however, the relevance of wild relatives of tomato has not yet been assessed. In this study, we found that S. pimpinellifolium accession To-937 (SP) may be a suitable resource to increase NUE in tomato. We studied a set of 29 introgression lines (IL) from SP into the Moneymaker cultivar (MM) in different seasons to investigate the potential of SP introgressions to maintain the tomato plant performance during the growth cycle under low N input in greenhouse conditions. We identified specific regions in the SP genome, on chromosomes 1, 3 and 10, involved in the responses to N inputs of fruit production and fruit quality. Notably, the line SP_10-4 maintained vegetative biomass and fruit yield production under limiting N supply. The introgressed region contained putative candidate genes as sucrose phosphate phosphatase (SPP), invertases (INV) and glutamine synthase 1 (GS1) genes, implicated in C and N metabolism. Genomic and expression analyses revealed differences in coding and non-coding sequences as well as in mRNA levels in SP_10-4, suggesting that these genes might well contribute to the reported biomass responses to N. Additionally, line SP_1-4 showed stable fruit amino acid contents under both sufficient and limiting N supplies, indicating that assimilated N partitioning to the fruit is maintained in response to N. Altogether, our results confirmed the suitability of SP as a source of NUE related traits and the interest in the studied ILs for developing new tomato cultivars with improved NUE under sustainable fertilization conditions.

Responses of soil microbial biomass C:N:P stoichiometry to increased precipitation and nitrogen deposition in temperate shrublands

Increased precipitation and nitrogen (N) deposition have considerable impacts on soil microbial communities in terrestrial ecosystems, ultimately influencing the carbon (C) cycling. However, the specific effects of the interactions between increased precipitation and N deposition on C:N:P stoichiometry in soil microbial biomass remain unclear. A long-term (9 yr) manipulated experiment was conducted to investigate the responses of soil microbial biomass C:N:P stoichiometry to increased precipitation and N deposition of the Desert shrubland in Gurbantunggut, northwest China. The results demonstrated that increased precipitation elevated C, N, and C:N ratios of microbial biomass by 26.9%, 18.7%, and 6.8% relative to ambient precipitation, respectively. In comparison to ambient N deposition, N addition resulted in a 37.5% increase in soil microbial biomass N and a 6.8% increase in the N:P ratio. Particularly, the interactive effects between increased precipitation and N deposition on microbial biomass P and N:P ratio were significant. Moreover, the influences of increased precipitation and N addition on microbial biomass C:N:P stoichiometry primarily occurred through changes in dissolved organic carbon content and extracellular enzyme activities. The findings underscore the crucial role of increased precipitation as a stronger driving force for variations in microbial biomass C:N:P stoichiometry in arid ecosystems, compared to N deposition.

Utilisation of press mud for organic cultivation of Andrographis paniculata Wall. ex Nees in reclaimed sodic soil

ABSTRACT Andrographis paniculata is a medicinal plant used to treat many different ailments. The biomass of A. paniculata can be used safely either as fresh plant material, after decoction, or in pharmaceutical formulations. In the present study, different application rates of press mud were evaluated for organic cultivation of A. paniculata in the Indo-Gangetic Plains of Uttar Pradesh, India. The vegetative plant growth parameters were positively affected by increased application rates of press mud up to 7.5 and 10 t ha-1. Macro- and micro-nutrient (N, P, Ca, Fe, Zn, Cu, Mn, Mo and Ni) concentrations of the plant tissue increased with the application of press mud. Yields of active compounds in the A. paniculate, i.e. andrographolide, neo-andrographolide and wogonin, increased in line with the increased total plant biomass in response to increased press mud application rates. Soil microbial populations, total heterogeneous bacteria, fungi, actinomycetes and Azotobacter also increased with increased rates of press mud, as did soil enzyme activities, i.e. dehydrogenase, β-glucosidase, urease, protease and acid phosphatase. Soil pH and bulk density decreased with increased rates of press mud, which was seen as indications of improved soil properties. Furthermore, available macro- and micro-nutrient concentrations in the soil increased with increased rates of press mud. The present study demonstrated that in organic cultivation of A. paniculata, optimised application rates of press mud can be used not only to supplement soil nutrients but also to enhance yields of plant biomass and active constituents of A. paniculata.

Physiological responses of typical subtropical landscape shrubs to artificial light at night.

Artificial light at night is rapidly spreading and has become an important component of global change. Although numerous studies have focused on its potential ecological impacts, the physiological response mechanisms of landscape plants to artificial light at night have rarely been quantified. With common landscape shrubs in subtropical regions of China, Hydrangea paniculata, Photinia fraseri and Ligustrum japonicum, as test materials, we exa-mined the responses of antioxidant enzyme system and biomass in the light environment at night under different light quality (yellow light, white light) with different light intensities (20, 40, 60 lx) . The results showed that artificial light at night significantly increased the membrane peroxidation, stimulated plant antioxidant protection systems and raised the antioxidant enzyme activities of the three species. The effects of light quality on plant antioxidant enzymes varied across dspecies. The peroxidase (POD) and catalase (CAT) activities of H. paniculata under white light were 1.5 and 1.3 times as that under yellow light, respectively. Both enzyme activities of P. fraseri were 1.1 times as that under white light than under yellow light. The activities of two enzymes in L. japonicum under white light were 88.6% and 99.5% of those under yellow light, respectively. The antioxidant enzyme activities of the three species increased with increasing light intensity at night, whereas the contents of malondialdehyde increased rapidly and the antioxidant enzyme activities decreased when beyond a certain light intensity threshold (at 120 d, the threshold was about 40 lx). The protective enzymes that played the major role under nighttime light stress were different among the three species. For H. paniculata, POD and CAT complemented each other to resist stress-induced oxidative damage, while the main enzyme of L. japonicum was POD. The biomass of the three species increased significantly under artificial light at night. H. paniculata was the most sensitive to nighttime light stress, while L. japonicum had the strongest resistance to the stress. The deciduous shrub H. paniculata could tolerate the white night light lower than 40 lx, while the evergreen shrubs P. fraseri and L. japonicum could tolerate the yellow night light lower than 40 lx.

Seed source environment predicts response to water availability in Plantago patagonica

The intensity, duration, and severity of drought increasing across the American Southwest. Plant restoration efforts are often thwarted by drought‐induced effects (i.e. drought‐induced mortality or failure to reproduce). Careful selection of plant materials to match future environmental conditions could improve restoration success under climate change. Here, we focus on Plantago patagonica, a priority arid land restoration species in the southwestern US. Using experimental drought in a greenhouse and supplemental watering in a common garden experiment, we quantified how the seed‐collection site environment influenced plant growth, performance, phenotypic plasticity, and evidence of local adaptation in 12 populations of P. patagonica. In the greenhouse, we found plants from hotter and drier environments had higher root:shoot ratios, while those from more variable precipitation regimes had greater total biomass in response to drought. Populations sourced from warmer environments exhibited sevenfold greater plasticity in root:shoot ratio compared to those from cooler environments, and there was strong evidence for local adaptation in phenology, as populations sourced from geographic locations nearer the common garden exhibited five times more flowering individuals than those sourced furthest from that garden. In the common garden, we found that plants sourced from wetter locations and those with more consistent precipitation regimes had lower mortality and higher specific leaf area, a proxy for growth, under unwatered ambient conditions. These results suggest plant performance of P. patagonica under reduced water availability is strongly shaped by the seed‐collection site environment and may be a useful tool for improving restoration outcomes in a changing world.

Differences in Fe toxicity response index and associated growth characteristics among rice genotypes

ABSTRACT Acid sulfate soils in tropical coastal rice areas contain high Fe levels, and excess ferrous (Fe2+) ions are released during the early growth stage in the wet season. In such environments, Fe toxicity can cause significant yield losses. The objectives of this study were to evaluate the seedling growth of diverse rice germplasm (from japonica, aus and indica groups) under high-Fe conditions in hydroponic culture and to elucidate the growth characteristics of Fe toxicity tolerant genotypes. We calculated an Fe toxicity response index (FTRI) as a selection criterion that reflects both the response of shoot biomass to high Fe and the amount of Fe-induced leaf bronzing damage: higher FTRI values indicate greater tolerance to high Fe levels. We first compared Fe levels to determine the appropriate conditions for genotype selection, and then screened 2- to 3-week-old seedlings grown for 2 more weeks in hydroponic solution containing 500 mg Fe2+ L−1. Sixteen genotypes were screened, and for five of them, the differences in FTRI corresponded with previously reported yield performance in high-Fe fields. We identified Taichung65 (temperate japonica) as a promising donor, as it showed little bronzing damage and high relative (stress/control) biomass under high-Fe conditions, resulting in a high FTRI. Plant nutrient analyses of Taichung65 suggested that maintenance of low Fe concentration and low total Fe content in shoots despite high Fe concentration in the rhizosphere, through prevention of either root Fe uptake or Fe translocation from the root to the shoot, should be an important mechanism of Fe toxicity tolerance.

Trophic amplification: A model intercomparison of climate driven changes in marine food webs.

Marine animal biomass is expected to decrease in the 21st century due to climate driven changes in ocean environmental conditions. Previous studies suggest that the magnitude of the decline in primary production on apex predators could be amplified through the trophodynamics of marine food webs, leading to larger decreases in the biomass of predators relative to the decrease in primary production, a mechanism called trophic amplification. We compared relative changes in producer and consumer biomass or production in the global ocean to assess the extent of trophic amplification. We used simulations from nine marine ecosystem models (MEMs) from the Fisheries and Marine Ecosystem Models Intercomparison Project forced by two Earth System Models under the high greenhouse gas emissions Shared Socioeconomic Pathways (SSP5-8.5) and a scenario of no fishing. Globally, total consumer biomass is projected to decrease by 16.7 ± 9.5% more than net primary production (NPP) by 2090-2099 relative to 1995-2014, with substantial variations among MEMs and regions. Total consumer biomass is projected to decrease almost everywhere in the ocean (80% of the world's oceans) in the model ensemble. In 40% of the world's oceans, consumer biomass was projected to decrease more than NPP. Additionally, in another 36% of the world's oceans consumer biomass is expected to decrease even as projected NPP increases. By analysing the biomass response within food webs in available MEMs, we found that model parameters and structures contributed to more complex responses than a consistent amplification of climate impacts of higher trophic levels. Our study provides additional insights into the ecological mechanisms that will impact marine ecosystems, thereby informing model and scenario development.

Responses of soil CO2 efflux and microbial activity to water deficit under conventional and adaptation technology

Frequent periods of low rainfall accompanying climate change are expected to affect many aspects of agricultural production and greenhouse gas emissions. In this work, we conducted field experiments on soil CO2 efflux and soil microbial activity using two precipitation treatments and two crop management technologies. While the control treatment was exposed to natural precipitation, the reduced water availability was induced during the early stages of crop growth using rain-exclusion shelters within the water deficit treatment. We aimed to test the difference in the response to water deficit between two management technologies: conventional tillage (CT - ploughed without cover crops) and adaptation technology (AT - no-till with species rich cover crops). After a month of simulated water deficit, the soil CO2 efflux under a regime of adaptation technology was essentially the same as that under the conventional technology, and the decrease in soil CO2 efflux ranged between 22 % and 55 %. The water deficit had a technology-specific effect on microbes. While no effect on the microbial carbon under CT was observed, the water deficit decreased Cmic under AT. In contrast, the opposite pattern was observed for DHA. When a normal soil water regime was restored, CO2 efflux recovered rapidly while recovery of microbial biomass and dehydrogenase activity was more variable. Generally, the soil microbial biomass and activity recovered better under AT. The results show that while the reduction in Rs during water deficit and its recovery after the restoration of the ambient precipitation regime were not affected by the technology, the response of microbial biomass and activity to water deficit depended on the technology with better recovery under AT.

Responses of Plant Species Diversity and Biomass to Forest Management Practices after Pine Wilt Disease

Pine wilt disease (PWD), which is caused by the Bursaphelenchus xylophilus, is one of the most serious biological invasions in East Asia. Removal of infected pine trees is generally used to prevent the spread of PWD. However, how this strategy affects plant species diversity and ecosystem functions needs to be clarified. We compared alpha diversity, species composition, and biomass for all woody plant species, adults, saplings, and seedlings in infected Masson pine forests where removal of infected trees occurred (CTD) and where infected trees were retained (UTD), uninfected Masson pine forests (PMF), and evergreen broad-leaved forests (EBF). UTD had the highest alpha diversity of all species and saplings, and UTD and PMF had the lowest alpha diversity of seedlings. CTD and EBF had a similar composition of seedlings, and CTD and UTD had similar species composition of all plant species and saplings. UTD had the lowest biomass for all species and adults but had the highest saplings biomass. Soil properties were significantly related to plant biomass. The retention of infected trees likely maintained soil fertility which increased species alpha diversity and the biomass of saplings, and unchanged species composition compared to the removal of infected trees, indicating that the retention strategy could help to maintain ecosystem functions.

Nitrogen fertiliser demand of canola is low when produced in conservation agriculture systems in South Africa

Current fertiliser guidelines for nitrogen (N) application for canola under conservation agriculture (CA) in South Africa, may result in over-fertilisation. This study aimed to evaluate the effects of N rates and foliar applied N on biomass, seed and oil yield of canola grown under CA. Trials took place from 2016 to 2019 at Darling, Porterville, Moorreesburg, Riviersonderend and Riversdale in the Western Cape, South Africa, representing diverse soil and climatic conditions. The study evaluated the application of 0, 25, 50, 75, 105, 135, 160 and 190 kg N ha−1 N rates using limestone ammonium nitrate followed by a urea ammonium nitrate foliar application of 20 kg N ha−1 vs a control (no foliar). The responses of biomass, seed and oil yield to top-dressed fertiliser N rate was site specific and varied with in-season rainfall. Foliar N application had no effect (p > 0.05) on biomass (all sites), seed yield (Darling, Porterville and Riversdale) and oil yield (Darling). At Moorreesburg (2016 and 2017) and Riviersonderend (2016, 2017 and 2019) foliar N increased seed yield. The lack of response by biomass, seed and oil yield to increased N application rates under different soil and rainfall conditions suggests lower fertiliser N demand under CA conditions.

Decadal soil warming decreased vascular plant above and belowground production in a subarctic grassland by inducing nitrogen limitation.

Below and aboveground vegetation dynamics are crucial in understanding how climate warming may affect terrestrial ecosystem carbon cycling. In contrast to aboveground biomass, the response of belowground biomass to long-term warming has been poorly studied. Here, we characterized the impacts of decadal geothermal warming at two levels (on average +3.3°C and +7.9°C) on below and aboveground plant biomass stocks and production in a subarctic grassland. Soil warming did not change standing root biomass and even decreased fine root production and reduced aboveground biomass and production. Decadal soil warming also did not significantly alter the root-shoot ratio. The linear stepwise regression model suggested that following 10 yr of soil warming, temperature was no longer the direct driver of these responses, but losses of soil N were. Soil N losses, due to warming-induced decreases in organic matter and water retention capacity, were identified as key driver of the decreased above and belowground production. The reduction in fine root production was accompanied by thinner roots with increased specific root area. These results indicate that after a decade of soil warming, plant productivity in the studied subarctic grassland was affected by soil warming mainly by the reduction in soil N.

Effects of biochar on soil microbial communities: A meta-analysis

Changes in soil microbial communities may impact soil fertility and stability because microbial communities are key to soil functioning by supporting soil ecological quality and agricultural production. The effects of soil amendment with biochar on soil microbial communities are widely documented but studies highlighted a high degree of variability in their responses following biochar application. The multiple conditions under which they were conducted (experimental designs, application rates, soil types, biochar properties) make it difficult to identify general trends. This supports the need to better determine the conditions of biochar production and application that promote soil microbial communities. In this context, we performed the first ever meta-analysis of the biochar effects on soil microbial biomass and diversity (prokaryotes and fungi) based on high-throughput sequencing data. The majority of the 181 selected publications were conducted in China and evaluated the short-term impact (<3 months) of biochar. We demonstrated that a large panel of variables corresponding to biochar properties, soil characteristics, farming practices or experimental conditions, can affect the effects of biochar on soil microbial characteristics. Using a variance partitioning approach, we showed that responses of soil microbial biomass and prokaryotic diversity were highly dependent on biochar properties. They were influenced by pyrolysis temperature, biochar pH, application rate and feedstock type, as wood-derived biochars have particular physico-chemical properties (high C:N ratio, low nutrient content, large pores size) compared to non-wood-derived biochars. Fungal community data was more heterogenous and scarcer than prokaryote data (30 publications). Fungal diversity indices were rather dependent on soil properties: they were higher in medium-textured soils, with low pH but high soil organic carbon. Altogether, this meta-analysis illustrates the need for long-term field studies in European agricultural context for documenting responses of soil microbial communities to biochar application under diverse conditions combining biochar types, soil properties and conditions of use.

Microbial Biomass and Soil Respiration Response to Pruning and Fertilization Practices in Coffee-Pine Agroforestry

Microbial Biomass and Soil Respiration Response to Pruning and Fertilization Practices in Coffee-Pine Agroforestry

3PG-MT-LSTM: A Hybrid Model under Biomass Compatibility Constraints for the Prediction of Long-Term Forest Growth to Support Sustainable Management

Climate change is posing new challenges to forestry management practices. Thinning reduces competitive pressure in the forest by repeatedly reducing the tree density of forest stands, thereby increasing the productivity of plantations. Considering the impact of thinning on vegetation and physiological and ecological traits, for this study, we used Norway spruce (Picea abies) data from three sites in the PROFOUND dataset to parameterize the 3-PG model in stages. The calibrated 3-PG model was used to simulate the stand diameter at breast height and the stem, root, and leaf biomass data on a monthly scale. The 3PG-MT-LSTM model uses 3-PG simulation data as the input variable. The model uses a long short-term memory neural network (LSTM) as a shared layer and introduces multi-task learning (MTL). Based on the compatibility rules, the interpretability of the model was further improved. The models were trained using single-site and multi-site data, respectively, and multiple indicators were used to evaluate the model accuracy and generalization ability. Our preliminary results show that, compared with the process model and LSTM algorithm without MTL and compatibility rules, the hybrid model has higher biomass simulation accuracy and shows a more realistic biomass response to environmental driving factors. To illustrate the potential applicability of the model, we applied light (10%), moderate (20%), and heavy thinning (30%) at intervals of 10, 15, 20, 25, 30 years. Then, we used three climate scenarios—SSP1-2.6, SSP2-4.5, and SSP5-8.5—to simulate the growth of Norway spruce. The hybrid model can effectively capture the impact of climate change and artificial management on stand growth. In terms of climate, temperature and solar radiation are the most important factors affecting forest growth, and under warm conditions, the positive significance of forest management is more obvious. In terms of forest management practices, less frequent light-to-moderate thinning can contribute more to the increase in forest carbon sink potential; high-intensity thinning can support large-diameter timber production. In summary, moderate thinning should be carried out every 10 years in the young-aged forest stage. It is also advisable to perform light thinning procedures after the forest has progressed into a middle-aged forest stage. This allows for a better trade-off of the growth relationship between stand yield and diameter at breast height (DBH). The physical constraint-based hybrid modeling approach is a practical and effective tool. It can be used to measure long-term dynamic changes in forest production and then guide management activities such as thinning to achieve sustainable forest management.