Biology Classes Research Articles

Comparative Effectiveness of Biologic Classes in Clinical Practice: Month 12 Outcomes from the International Observational Psoriasis Study of Health Outcomes (PSoHO)

Comparative Effectiveness of Biologic Classes in Clinical Practice: Month 12 Outcomes from the International Observational Psoriasis Study of Health Outcomes (PSoHO)

Comparative Efficacy and Safety of TNF-Alpha Inhibitors, IL-17 Inhibitors, and IL-23 Inhibitors in the Treatment of Moderate to Severe Psoriasis: A Systematic Review

Psoriasis is a chronic inflammatory skin disease that significantly impacts patients' quality of life. The advent of biological therapies has revolutionized the treatment landscape for moderate to severe psoriasis, offering targeted mechanisms of action with higher efficacy and improved safety profiles compared to traditional systemic treatments. This systematic review aims to evaluate and compare the efficacy and safety of three major classes of biologics: TNF-alpha inhibitors, IL-17 inhibitors, and IL-23 inhibitors. We conducted a comprehensive search of PubMed, MEDLINE, and Cochrane Library databases for randomized controlled trials (RCTs) and observational studies published between 2015 and 2023. A total of 30 studies involving over 15,000 patients were included. The primary outcomes assessed were the Psoriasis Area and Severity Index (PASI) 75, 90, and 100 response rates and safety profiles. IL-23 inhibitors demonstrated the highest efficacy, with superior PASI 90 and PASI 100 responses, followed by IL-17 inhibitors. TNF-alpha inhibitors, while effective, showed lower PASI 90 and PASI 100 response rates but had a well-established long-term safety profile. IL-17 inhibitors were associated with higher rates of candidiasis, while IL-23 inhibitors had fewer adverse events overall. This review supports the use of IL-23 inhibitors as the most effective biological therapy for moderate to severe psoriasis, with IL-17 inhibitors as a strong alternative. TNF-alpha inhibitors remain a viable option, particularly for patients with comorbid conditions where their safety profile is well-documented.

Anti-IL4Ra therapy is superior to other biologic classes in treating allergic bronchopulmonary aspergillosis.

Anti-IL4Ra therapy is superior to other biologic classes in treating allergic bronchopulmonary aspergillosis.

Local translatome sustains synaptic function in impaired Wallerian degeneration.

After injury, severed axons separated from their somas activate programmed axon degeneration, a conserved pathway to initiate their degeneration within a day. Conversely, severed projections deficient in programmed axon degeneration remain morphologically preserved with functional synapses for weeks to months after axotomy. How this synaptic function is sustained remains currently unknown. Here, we show that dNmnat overexpression attenuates programmed axon degeneration in distinct neuronal populations. Severed projections remain morphologically preserved for weeks. When evoked, they elicit a postsynaptic behavior, a readout for preserved synaptic function. We used ribosomal pulldown to isolate the translatome from these projections 1 week after axotomy. Translatome candidates of enriched biological classes identified by transcriptional profiling are validated in a screen using a novel automated system to detect evoked antennal grooming as a proxy for preserved synaptic function. RNAi-mediated knockdown reveals that transcripts of themTORC1 pathway, a mediator of protein synthesis, and of candidate genes involved in protein ubiquitination and Ca2+ homeostasis are required for preserved synaptic function. Our translatome dataset also uncovers several uncharacterized Drosophila genes associated with human disease. It may offer insights into novel avenues for therapeutic treatments.

The effectiveness of teaching human evolution to 14-15-year-olds in Slovenia Učinkovitost poučevanja evolucije človeka pri 14-do 15-letnikih v Sloveniji

In Slovenia, human evolution is first taught in biology classes to 14–15-year-old students. For many of them, this will be the only contact with this topic at school. The objective was to determine how much knowledge students acquired about human evolution. Data were collected with a questionnaire using a 5-point Likert scale. The participants were 13–14-year-olds who had not yet learned about human evolution (the control group) and 15–16-year-olds who had learned about human evolution one year earlier in biology classes (the experimental group). The results show that students significantly improved their knowledge of human evolution with small to medium effect sizes. Students knew very well that modern humans are the only species of humans today and that they did not live at the same time as dinosaurs. The achievements were low on the following topics: modern humans lived at the same time as Neanderthals and mammoths, and modern humans did not evolve from Neanderthals. To the problematic topics, attention should be paid to teacher education and the primary school curriculum. There were few correlations between the knowledge of human evolution, acceptance of evolution, religiosity, and attitudes toward biology.

Pre-vocational education as a factor in the development of human resources in the healthcare system

Pre-professional medical education in high school provides not only a more in-depth study of individual subjects of general education programs, but also expands the opportunities for self-determination of students, ensures continuity of the educational process, allows boys and girls to adapt more successfully mastering higher medical professional education programs and is the basis for a successful career.Aim. To evaluate the results of the introduction and implementation of the model of pre-professional medical and pharmaceutical education using the example of the Resource Center «Medical Sechenov Pre-University» of the Sechenov First Moscow State Medical University (Sechenov University) (hereinafter referred to as the Pre-University) as a means of developing the human resources potential of the healthcare system on the territory of the Russian Federation.Materials and methods. During the work, a theoretical analysis of domestic and foreign literature on the problem of developing the human resources potential of the healthcare system was carried out. A study was conducted in the comparative aspect of the basic readiness of students in grades 8-11 at the Pre-University and specialized medical and biological classes of regional educational institutions to the choice of medical specialties using a test questionnaire with the identification of three key aspects: readiness to master medical specialties; self-assessment of capabilities and erudition in the field of modern medicine. 1465 people took part in the survey. The reliability of the results was carried using the Mann-Whitney U test. Assessment of the statistical significance of differences between the means was carried out at a critical level of p = 0.05.Results. Studying at the Pre-University of Sechenov University allowed the majority of adolescents to achieve higher levels of formation of the main indicators of basic readiness to choose medical specialties. The share of students with a high level of readiness to master medical specialties at the Pre-University is 13.8 % higher compared to students of specialized medical and biological classes, with high erudition – 13.8 % and 6.7 % higher those who highly assess their prospects for entering a medical university.Conclusion. Pre-professional education based on close interaction between the Pre-University and Sechenov University allows students to develop motivation to choose a medical profession, as well as a clear understanding of the learning process in the chosen profession and is an important link in the system of continuous educational trajectory «pre-university–university–clinic».

Factors Affecting Treatment Persistence in Japanese Patients with Psoriasis Prescribed Biologics: A Real-World Study Using an Insurance Claim Database.

Poor persistence to biologics can result in suboptimal health outcomes and increased economic burden for chronic conditions, including psoriasis (PsO). In Japan, studies evaluating factors responsible for biologic treatment persistence in patients with PsO are limited. We assessed biologic treatment persistence (median treatment duration and overall treatment survival) and associated factors in patients with PsO in a real-world setting. This retrospective analysis of insurance claims records from the Japan Medical Data Center (JMDC) database included patients with PsO [International Classification of Diseases (ICD) code: L40.x] ≥ 18years of age who had received biologic treatment. Treatment persistence was analyzed using data from 2016 to 2020 by biologic class and by individual biologics (infliximab, adalimumab, ustekinumab, guselkumab, secukinumab, ixekizumab, and brodalumab) in bio-naïve (who initiate first biologic at index) and bio-experienced patients. Kaplan-Meier survival (treatment persistence), and multivariate Cox proportional hazard regression (predictive factors) analyses were used. Overall, 1528 patients with PsO were included (mean age 47.4years). Infliximab had the longest median treatment duration (33.6months), while brodalumab had the shortest (9.7months) among biologics evaluated. Of the biologics evaluated, 1-year treatment survival was highest with guselkumab (83%), and lowest with brodalumab (45%). Bio-experienced patients showed slightly longer median treatment duration than bio-naïve patients (22.8 versus 18.1months). Factors predictive of treatment persistence were sex [male; hazard ratio (HR) 0.84, p = 0.016] and specific PsO diagnostic codes, such as L40.0 (PsO vulgaris; HR 0.69; p = 0.006), L40.1 (generalized pustular PsO; HR 0.75; p = 0.034), and L40.9 (PsO unspecified; HR 0.72; p = 0.001). Meanwhile, age and Charlson Comorbidity Index score were significantly associated with adalimumab and infliximab treatment persistence, respectively. Among biologics evaluated, infliximab had the longest median treatment duration, and guselkumab had the highest 1-year treatment survival. Sex and specific PsO diagnostic codes influenced overall treatment persistence. These findings could inform long-term treatment plans for PsO in real-world clinical settings.

ICT in biology education: teachers’ readiness and implementation challenges in the context of Bangladesh

Information and Communication Technology (ICT) is commonly employed in biology classes to facilitate students' comprehensive comprehension of biological processes and enable them to see living phenomena. The purpose of the study was to explore the use of ICT in enhancing the teaching and learning process of biology in the secondary schools of Bangladesh. In our study, sequential explanatory mixed method design has been followed. Data was collected from purposively selected ten secondary schools of Dhaka city. The study reveals that teachers have a positive attitude towards incorporating ICT in biology lessons and possess operational understanding of data processing tools and online browsing. Nevertheless, it also emphasizes the fact that teachers are devoid of personal computers in their houses and have restricted availability to school computers. Furthermore, the existing curriculum and teachers' guides lack adequate instructions for integrating ICT into biology lectures. The study also suggests that the process of developing ICT-based curriculum is complicated, which deters teachers from incorporating ICT into their classes. Furthermore, there is a limited availability of educational materials related to ICT in the Bangla language. This study recommends continuous in-servicing training on ICT to teachers, interest free loan to teachers for purchasing laptops, developing interactive biology educational ICT contents in Bangla and include specific instructions in the curriculum and teachers’ guide on how to use ICT in biology classes. These findings have important implications for policy makers, educators, and researchers who are dedicated to expanding the use of ICT in education.

Validating an instrument for measuring community cultural wealth with biology majors at a Hispanic-serving institution.

Boosting underrepresented student persistence in STEM majors has been of interest for several years. Prior research has explored various factors that may influence student success and persistence in STEM majors. Specifically, some of these studies have employed Yosso's community cultural wealth framework to explore how a student's cultural wealth may link to certain outcomes. Most of these studies have taken a qualitative approach when exploring cultural wealth and less adopt a quantitative approach. Using biology students in an introductory biology class during the fall (n = 303) and spring semesters (n = 215) at a large Hispanic-serving institution in the southeastern United States, this study seeks to contribute to the literature by validating a previously constructed instrument with a new population. An exploratory factor analysis (EFA) was conducted using principal axis factoring and an oblique rotation. EFA results revealed 10 dimensions of cultural wealth. Additionally, confirmatory factor analysis confirmed that the model produced properly measures the constructs as intended. Overall, the final 56-item instrument used in this study was validated and can be used for measuring cultural wealth in similar populations.

Reduced Effectiveness of Anti-IgE Treatment Among Adults with Severe Asthma with Older Age of Asthma Onset: Results from the CHRONICLE Study.

Younger age of asthma onset (AAO) has been associated with an allergic phenotype, whereas eosinophilic phenotypes have been associated with older AAO. In randomized trials, biologic efficacy among adults with severe asthma (SA) has varied by age at asthma onset. To determine whether these associations observed in trials apply to real-world outcomes, this study examined biologic effectiveness by AAO and biologic class in a large, real-world cohort. CHRONICLE is an ongoing, real-world study of US adults with subspecialist-treated SA receiving biologics, maintenance corticosteroids, or who are uncontrolled on high-dosage inhaled corticosteroids with additional controllers. Patients enrolled between February 2018 and February 2022 who initiated a biologic for SA and had complete data for analysis were included. A locally estimated scatterplot smoothing (LOESS) analysis was used to plot the relationship between percentage exacerbation rate reduction and AAO by biologic class. Of 578 patients with complete data, 198, 149, and 231 were diagnosed with asthma at age <18, 18-39, and ≥40 years, respectively. Across subgroups, patients were predominantly White (72-78%), female (67-73%), and commercially insured (54-71%). In the LOESS analysis, exacerbation rate reductions were similar for anti-IgE and anti-IL-5/5R and anti-IL-4R subgroups with younger AAO, but the exacerbation rate reduction diminished for patients with older AAO receiving anti-IgE therapy, particularly with asthma onset age ≥40 years. Clinicians should consider age of onset in biologic treatment decisions, given reduced effectiveness of omalizumab in patients with asthma onset at age ≥40 years. NCT03373045.

The Impact of Psoriasis Treatments on the Risk of Skin Cancer: A Narrative Review.

Several studies have described increased risk ratios of certain types of malignancies in patients with severe psoriasis. Among these, the lymphoproliferative disorders, including non-Hodgkin's lymphoma, cutaneous T-cell lymphoma and non-melanoma skin cancer, have been described most frequently. In addition to traditional cancer risk factors, some psoriasis treatments may also be implicated as potential carcinogens. The aim of this study was to perform a review of current literature on the association between psoriasis, the therapies against this disease and skin cancer, focusing on both epidemiology and the potential mechanism involved. Some psoriasis treatments, such as psoralen and ultraviolet A (PUVA) therapy and cyclosporine, have been associated with increased risk of skin cancer. Variable data have been reported for anti-tumour necrosis factor (TNF) drugs, whereas other class of biologics, like anti-IL17 and IL23, as well as ustekinumab, seem not to be related to skin cancer risk, such as the case of currently available small molecules.

Disease response in rheumatoid arthritis across four biologic therapies associates with improvement in paraoxonase-1 activity and oxylipins

ObjectiveParaoxonase-1 (PON1) is a high-density lipoprotein (HDL)-associated enzyme, that has been implicated as a biomarker of cardiovascular risk in patients with rheumatoid arthritis (RA). We aimed to investigate how different...

Improving Students' Fluent and Flexible Thinking Skills through the Problem-Based Learning (PBL) Model Based on Technological Pedagogical and Content Knowledge (TPACK) in High School Biology Classes

Improving Students' Fluent and Flexible Thinking Skills through the Problem-Based Learning (PBL) Model Based on Technological Pedagogical and Content Knowledge (TPACK) in High School Biology Classes

Self-efficacy inertia: The role of competency beliefs and academic burden in achievement

The effort to translate self-efficacy theory into statistical models has been argued to model out the complex, dynamic nature of the interaction between the person and the environment. This study aimed to understand how self-efficacy (belief in one’s abilities) and academic burden (the external challenges students face in their studies) relate to academic performance over time and whether modeling both between and within subject variance components provides a more comprehensive perspective that is better aligned with theory. Self-efficacy and academic burden were collected at five time points, one month apart from undergraduate students (N = 443) enrolled in an online biology class. The data were fit to four models: 1) a standard cross lagged panel model (CLPM), 2) a random intercept cross-lagged panel model (RI-CLPM), 3) an RI-CLPM in which grade was regressed on the random-intercepts, and 4) an RI-CLPM in which grade was regressed on the random-intercepts and the within-person fluctuations. The RI-CLPM was a better fit to the data over the CLPM, indicating that separating effects that are attributed to individual differences from within-person effects appears to better capture the reciprocal relationships between self-efficacy and academic burden. Further, when only the general tendencies of self-efficacy and academic burden were specified to predict final grade, there was a significant positive relationship between self-efficacy and grades. However, when within-person variations over time were added as predictors in addition to the between-person differences, this general relationship lost significance. These findings suggest that gaining self-efficacy momentum in a class is perhaps more predictive of academic achievement than having a general tendency towards confidence in one’s abilities relative to peers.

Impact of climate, land management and harvesting patterns on the ecological traits and the population structure of Pterocarpus lucens in West African semi-arid areas

Multipurpose woody plant species experience severe pressure owing to human overexploitation, land management and climate change. In response to their inability to cope with environmental disturbances in their natural range, some species such as Pterocarpus lucens migrate to more suitable areas. To better conserve P. lucens, this study examined the impacts of climate, land management and harvesting practices on its natural populations.This study was conducted in Burkina Faso using a combined gradient approach involving two climatic zones and two land management types. Data on the biological age classes, structural traits and intensities of harvesting were collected on each individual of the species. Linear models were used to assess the effects of the investigated gradients on structural traits of the species. The diameter and height class size distributions were evaluated using the least squares method.The results showed that the species’ pre-reproductive individuals increased significantly (p < 0.05) with climatic and the protection gradient, resulting in an invasive trend of P. lucens in the Sudano-Sahelian protected areas. The species is overexploited in unprotected areas, with the majority of individuals undergoing all pruning intensities. However, while in the Sahelian climatic zone, decreasing pruning rates were observed according to the severity gradient (low intensity 36.02 %, moderate intensity 27.47 %, and severe intensity 19.73 %), an increasing pruning rate was observed in the Sudan-Sahelian climatic zone (low intensity 16.49 %, moderate intensity 17.38 %, severe intensity 24.48 %, and very severe intensity 35.79 %). Linear regression analysis showed a significant effect (p < 0.05) of climate, land management, pruning, and debarking on the structural traits of the species. The diameter class structure revealed unstable populations of the adult stratum in the Sahelian climatic zone, regardless of the land management types. The juvenile stratum of this species exhibited an unstable structure in unprotected areas, regardless of climatic zone.This study highlights the urgent need to implement effective management strategies to mitigate the decline of P. lucens populations in the Sahelian climatic zone and in unprotected areas through its integration in reforestation programmes.

Expert views on screening for tuberculosis infection in patients commencing treatment with a biologic agent.

Many biologic agents cause some degree of immunosuppression, which can increase the risk of reactivation of tuberculosis infection (TBI). This risk is variable between individual biologics. We aimed to assess current (and recommended) clinical practice of TBI screening and treatment among patients initiating treatment with biologic agents. An online questionnaire was distributed via email to members of the Global Tuberculosis Network and associated professional organisations to seek insights into the screening for and treatment of TBI in patients treated with biologics. A total of 163 respondents in 27 countries answered at least one question. For all biologics described in the questionnaire, respondents advised increasing screening relative to current practice. Observed and supported TBI screening rates in patients treated with TNF-a inhibitors were high, especially for older TNF-a inhibitors. Most participants supported TBI screening in patients treated with B- or T-cell inhibitors but not in those treated with interleukin inhibitors. Guideline awareness was higher for TNF-a inhibitors than for other biologic classes (79% vs. 34%). Although respondents stated that TBI screening rates are lower than what they consider ideal, there was a tendency to recommend TBI screening in patients treated with biologics not known to be associated with an increased risk of TBI. As a result, there is a potential risk of over-screening and over-treatment of TBI, potentially causing harm, in patients treated with biologics other than TNF-a inhibitors. There is a need to research the risk of TBI associated with biologics and for guidelines to address the spectrum of TBI risk across all types of biologics.

Sequencing of Targeted Therapy in Psoriasis: Does it Matter?

With the continued development of biologics for the treatment of psoriasis, some patients have achieved optimal control, but a recommended biologic sequence if a biologic fails to initially improve the skin, termed primary nonresponse, or loses efficacy after initial improvement, termed secondary nonresponse, is still lacking. Primary and secondary nonresponse can occur with any class of biologics, and the type of nonresponse can drive the choice of whether to switch within a biologic class or to a different biologic class. The choice of biologic can also be challenging when managing psoriasis and concomitant psoriatic arthritis, as treatment differs on the basis of the severity of both diseases and further classification of axial and peripheral joint involvement. When choosing a biologic, each patient's comorbidities and preferences are also taken into account to provide the optimal therapy. With this lack of anestablishedbiologic sequence after biologic failure, the objective of our review is to define a therapy sequence for the tumor necrosis factor (TNF), interleukin-17 (IL-17), and interleukin-23 (IL-23) inhibitor classes in the treatment of psoriasis and psoriatic arthritis. Our proposed biologic sequence was derived through an analysis of the efficacy of each biologic class, primary and secondary nonresponse rates from clinical trials, and clinical experience with expert opinion.

METODA CLIL S VYUZITIM AKTIVIZUJUCICH VYUCOVACICH METOD NA HODINACH BIOLOGIE

The preliminary research explores the implementation of the CLIL (Content and Language Integrated Learning) method in seventh-grade Biology classes at two primary schools, employing activating teaching methods. This paper comprehensively describes the CLIL method, including its historical background, various forms, types, and objectives. We analyze the advantages and disadvantages of CLIL, along with clarifying the role of the Biology teacher within this pedagogical framework. The study involves preliminary research conducted in two different elementary school classes: one without a specific language focus and one where students have a higher language proficiency. The main objective of the study was to determine changes in student attitudes following the implementation of the CLIL method. Through data analysis in action research, pre- and post-questionnaire, the study aims to identify any shifts in student attitudes after lessons conducted using the CLIL approach with activating teaching methods. Additionally, the research includes an analysis and evaluation of student preferences regarding the instructional methods used and the challenges faced by educators in implementing this method.

51784 Comparative Effectiveness of Biologic Classes in Clinical Practice: Month 12 Outcomes from the International Observational Psoriasis Study of Health Outcomes (PSoHO)

51784 Comparative Effectiveness of Biologic Classes in Clinical Practice: Month 12 Outcomes from the International Observational Psoriasis Study of Health Outcomes (PSoHO)

An exploration of the relationship between active learning and student motivation in STEM: a mixed methods study.

Much of the research on science, technology, engineering, and mathematics (STEM) students' motivation measures the relationship between student motivation and academic outcomes, focusing on the student's mindset. Our mixed-methods research takes a different approach and considers the relationship between student motivation and instructional practices. Teaching practices and student motivation were analyzed simultaneously in undergraduate Biology classes using a self-determination theory-based survey to measure students' motivation during courses that were observed using the Classroom Observation Protocol for Undergraduate STEM (COPUS), and observation notes were collected to document instructor and student behaviors. Quantitative data were used to differentiate students' motivational levels, and qualitative data were collected to describe how instructors use specific teaching practices. The results provide a lens into how students' intrinsic motivation varies alongside the instructional practices and interactions in these classes. We found a correlation between higher levels of student motivation in interactive lectures and student-centered teaching profiles. This study highlights how the same practice can be implemented by multiple instructors with varying student motivation scores, pointing out the importance of fidelity to evidence-based instructional practice methods. The results of this study are discussed in the context of published empirical studies examining evidence-based instructional practices that are conceptually supportive of autonomy, competence, and relatedness. Active learning practices observed in this study correlated to positive learning outcomes are discussed and may serve as a guide for instructors interested in implementing specific active learning practices. Recommendations for instructors and departments that are interested in flexible methods to monitor progress toward active learning practices in biology and other STEM disciplines by combining the COPUS and self-determination survey results are presented.NEW & NOTEWORTHY This study uses a novel combination of instruments to describe students' intrinsic motivation in response to teaching practices. Findings demonstrate that active learning methods may support higher student motivation. Recommendations drawn from the study include using a variety of active learning methods, using evidence-based instructional methods with fidelity, and monitoring the students' affective response to those methods. Alignment of active learning practices to the components of self-determination may result in higher quality student motivation in science, technology, engineering, and mathematics (STEM) courses.