Biological Value Protein Research Articles

Methodology for comparative evaluation of high-protein feed for farm animals and poultry

The peculiarity of the proposed methodology is the inclusion in the evaluation criteria of indicators that characterise the biological value of protein (content of protein convertible into animal products, non-degradable protein) and its cost. Approbation of the methodology made it possible to build a rating of the most preferable forages for each type of farm animals and poultry both from the position of satisfying their physiological needs in nutrients and taking into account the need to reduce production costs.

Nutritional properties of commercially important cephalopods from the south-west coast of India

Cephalopods are widely regarded as healthy and nutritionally rich seafood resources. This study focuses on analysing the nutritional composition of six commercially important edible cephalopod species viz., Cistopus indicus, Amphioctopus marginatus, Amphioctopus aegina, Sepia pharaonis, Sepia elliptica and Sepia aculeata, from the Malabar coast of the Indian peninsula. The selected cephalopods were determined to be a rich source of protein ranging from 12.41 to 19.02 g 100 g-1. The amino acid profile demonstrated a balanced ratio of essential to non-essential amino acids (0.93-1.63) with a higher concentration of essential amino acids, attributing enhanced biological value of proteins in these cephalopods. S. pharaonis recorded the highest arginine-to-lysine ratio (~1.77), indicating excellent cholesterolemic index. Notably, octopus species had the lowest total cholesterol content (~100 mg 100 g-1) compared to cuttlefish species which recorded higher cholesterol levels (~160 mg 100 g-1). A. marginatus recorded more significant quantities of polyunsaturated fatty acids (PUFA) (36.85% total fatty acids) among all the species studied. Octopus species exhibited significantly greater Σn-3/Σn-6 ratio than cuttlefish species. The ideal atherogenicity/thrombogenicity indices (<1.0) in the edible tissues of the studied cephalopods qualify these species as potentially healthy food. A. aegina was found to exhibit greater vitamin E (α-tocopherol) contents (17.84 IU), whereas ascorbic acid was pronounced more in A. marginatus (47.06 μg 100 g-1). The higher vitamin D3 content in A. aegina (96.23 μg 100 g-1) among the species studied, highlights its potential role in preventing osteoporosis in adults. Among microelements such as iron and zinc were found in significant quantities in the cephalopod species studied. The findings from the current study provide valuable insights into the nutritional benefits of these species, positioning them as promising candidates for the seafood industry as possible healthy dietary options. Keywords: Amino acid composition, Atherogenicity index, Cephalopods, Fatty acid composition, Thrombogenicity index

Impact of early weaning and nutritional interventions on growth performance, digestive metabolism, and serum biomarkers in lambs: A biomechanical perspective

The aim of this study was to investigate the effects of early weaning and nutritional interventions on growth performance, nutrient digestion and metabolism, and serological indices of hu sheep lambs, with a view to providing a basis for the feasibility of early weaning of hu sheep lambs under conditions of supplemental milk replacer. Ninety neonatal hu sheep lambs were weaned at 21 days (n = 60) (divided into two groups: early weaned, EW; and resveratrol-fed, RSV) as well as 49 days (n = 30) (control group, CON) weaning, and the trial period was 90 days. The results showed that: 1) there was no significant difference (P > 0.05) in the initial and final weights of the lambs, and the average daily gain (ADG) of the EW and RSV groups was significantly lower than that of the CON group (P < 0.05) from 31 to 45 days of age. Early weaned lambs were more susceptible to weaning stress compared to late weaned lambs and the effect of nutritional intervention (feeding resveratrol) on lamb growth performance was not significant. 2) The apparent digestibility of crude protein (CP) in the experimental group was significantly lower than that of the control group (P < 0.05), and the nitrogen intake, net protein utilization and protein biological value indexes of lake lambs in the experimental group were significantly different from those of the control group (P < 0.05). 3) Early feeding of resveratrol was not significant in improving the digestive metabolism of nutrients. 4) Early weaning as well as the addition of resveratrol had a significant effect on the serum GLU and TC indexes of the lambs (P < 0.05), but did not show a significant effect on any of the other indexes.

Dietary supplementation of black cumin (Nigella sativa) meal in the formulation of protein-enriched cookies, further invivo evaluation of protein quality with physicochemical and organoleptic characterization.

Nigella sativa has solid historical importance as a medicinal herb and is widely used in the bakery industry. The study revealed that the defatted black cumin meal (DBCM) possesses a high nutritious composition and amino acid profile, such as Leu, Lys, Arg, Phe, and Glu. The supplementation effect of wheat flour with defatted black cumin meal (DBCM) at levels of 5%-25% was explored on the cookies' nutritional, functional, and organoleptic attributes. Moreover, the present work was conducted to assess the invivo protein quality of defatted black cumin meal (DBCM)-supplemented cookies using the albino rat's experimental modeling for 10 days. Rheological characterization suggested a solid elastic behavior for all cookies dough with elevation in G' (138,652-230,926 Pa), G" (34201-45,092 Pa), and G* values due to protein addition. The functional characteristics of the flour blends showed that DBCM addition significantly (p ≤ .05) improved oil (70.56%-165%) and water (84.83%-232.67%) absorption capacities, emulsion, and foaming stability. The physicochemical and organoleptic assessment of the cookies exposed that a 20% level of substitution of DBCM produced acceptable cookies. The net protein utilization (NPU), protein efficiency ratio (PER), true digestibility (TD), and biological value (BV) significantly differ from each other among all the supplementation diet groups with 0%-25% DBCM.

Investigation of the amino acid composition of gluten-free biscuit with carob

The article studies the amino acid composition of the developed biscuit semi-finished products. When developing the recipe for gluten-free products, special attention was paid to the protein composition of the raw materials and the calculation of the amino acid score, since the lack of a certain amino acid affects the ability of gluten formation during the kneading process. In addition, an unbalanced amino acid composition can cause an imbalance in the human body and negatively affect the functioning of muscles, heart, brain and other organs, leading to diseases. The objectives of the research included the following: determination of the amino acid composition of gluten-free biscuit semi-finished products; analysis of amino acid composition and calculation of the amino acid score of gluten-free biscuit products. The amino acid composition was calculated according to well-known methods, taking into account the chemical composition of food products. During the calculation, the biological value of proteins was assessed, the utility coefficient, the amino acid composition balance coefficient, and the amino acid score difference coefficient were determined. It was established that the developed gluten-free biscuit contained a significant amount of threonine, namely 11.38 g per 100 g of protein. As a result of calculations, it was determined that the first limiting amino acid was tryptophan, the second was the compound «phenylalanine + tyrosine», the shares of which in the amino acid score were 0.12 and 0.90, respectively. An assessment of the complex balance of a gluten-free biscuit semi-finished product showed an insufficient level of balance in human nutrition, but the indicators of individual amino acids compared to the control made from wheat flour were higher, for example, valine by 1.8 times, isoleucine, leucine and lysine by 1.1 times, metheonine + cysteine 2.7 times and threonine 2.8 times.

Study of the effect of onion husk ethanol extract on the chemical composition and microstructure of meat pates

The wide use of antioxidants is due to their involvement in free radical processes in foods and human body. Interest in the use of low-value raw materials providing products with functional properties and increasing their shelf life is rapidly increasing. However, any changes in the formulation and technology may affect the properties and composition of the finished product. During the work, the effect of replacing 34% (sample 1) or 17% (sample 2) beef broth with 70% water-ethanol extract of yellow onion peels in the formulation of the experimental meat pates was investigated. The control product contained only beef broth as liquid. The total antioxidant capacity by the DPPH radical method (TACDPPH), fatty acid composition and amino acid composition were determined; microelement content analysis, proteomic and microstructural studies of meat pate samples with and without the addition of extract were also carried out. For 14 days, TACDPPH values of experimental pates were higher than in control by at least 2.32 times (P<0.10). Samples 1 and 2 were characterized by a decrease in the concentrations of zinc, manganese and magnesium by no more than 14% (P<0.10), with a simultaneous increase in selenium, copper, potassium and calcium of 8% to 17.35% (P<0.10) depending on the microelement. The mass fraction of protein in experimental pates 1 and 2 was higher by 6.76% and 2.73% (P<0.10), respectively, which was due to a decrease in moisture because of ethanol evaporation. Replacing the broth in the formulation affected the decrease in the protein biological value, as evidenced by a decrease in amino acid scores (AASs). However, a decrease in the AAS difference coefficient in experimental pates 1 and 2 by 7.71% and 3.07%, respectively, led to an increase in the biological value of the pates by 7.7% and 3.06%, respectively. Based on the results of proteomic and histological analysis, it was revealed that the addition of ethanol extract did not lead to significant changes in the protein composition and microstructural characteristics of the test samples.

Macronutrient Analysis and In vivo Test in the Utilization of Barracuda Fish (Sphyraena barracuda) Asap Liquid as an Action to Meet Protein Needs

The smoking process has undergone significant development and liquid smoke has been widely used for the food industry as an ingredient that gives a distinctive aroma and flavor to food products. The purpose of this study was to determine the macronutrient quality of smoked fish and protein absorption in mice. Quality testing of barracuda smoked fish refers to SNI 2725: 2013. Test types include water content, ash content, protein content, fat content, pH, and Benzo[a]pyrene. In vivo analysis was conducted on mice to observe Protein Efficiencey Rate (PER), Net Protein Ratio (NPR), Total Protein Digestibility (TPD), Biological Value (BV), and Net Protein Utilization (NPU) values. Data were analysed with One-Way ANOVA. The results showed that the use of liquid smoke at a concentration of 5% had a significant effect (p < 0.05) on the test of moisture content of 55.2 ± 0.50, ash content of 4.28 ± 0.02, protein content of 35.47 ± 0.23, fat content of 4.55 ± 0.08, pH 6.77 ± 0.05 and benzo[a]pyrene < 0.02. The parameter results of TPD 78.21 ± 31.87, NPU 80.43 ± 3.75 in liquid smoked barracuda fish have higher values than traditional smoked barracuda fish, while PER 3.6 ± 4.06, NPR 4.06 ± 1.76 and BV 200 ± 4.24 have higher values in liquid smoked barracuda fish compared to traditional smoked barracuda fish. The addition of liquid smoke can to maintain product quality and improve the nutritional quality of protein because protein can be digested and absorbed by the body properly.

The impact of the distribution method for struvite (Crystal Green) on the chemical composition of soybean and their utility in animal nutrition

One of the main factors considered in assessing the nutritional value of feed is its chemical composition, which can be modified by fertilization. Faced with reducing P resources, alternative sources of this element are being sought. Phosphorus is an essential nutrient in soybean cultivation. The aim of the study was to use an alternative source of phosphorus fertilizer and compare its impact on the chemical composition of soybean seeds with that of a traditional fertilizer (Super FOS DAR). The study investigated a range of factors in animal nutrition as well as the basic content of macro- and microelements. A pot experiment with the Abelina soybean variety was conducted at the Experimental Station of the Wroclaw University of Environmental and Life Sciences. The experiment considered two factors against the control: phosphorus fertilizer placement (band, broadcast) and different phosphorus fertilization (Super FOS DAR, Crystal Green). Use of struvite (Crystal Green)) caused positive changes in selected amino acids content and in the nutritional value of protein in soybean seeds; this can enhance the value of soybean seeds as well as increase certain macroelements and microelements. Phosphorus fertilizer significantly increased the content of lysine, leucine, valine, phenyloalanine and tyrosine. Band fertilization with struvite caused a significant increase in amino acids (lysine, leucine, valine, phenyloalanine and tyrosine) as well as in the nutritional value of protein (as measured by the essential amino acid index, protein efficiency ratio and biological value of the protein). Favorable changes under the influence of the application of struvite were recorded in the content of calcium, as well as phosphorus, iron, and manganese. The value of the struvite in the case of its use as phosphorus fertilizer is promising; however, it needs further study.

Including dietary valine and tryptophan, but not isoleucine, above the requirement for growing pigs may partly ameliorate negative effects of excess leucine from corn protein on nitrogen balance and growth performance

Two experiments were conducted to test the hypothesis that increasing concentrations of dietary L-valine, L-isoleucine, or L-tryptophan in diets containing excess leucine (Leu) from corn protein will alleviate negative effects of excess dietary Leu on N balance and growth performance of growing pigs. In Exp. 1, 72 barrows (body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly allotted to 8 dietary treatments in a 12-day experiment. A basal diet based on corn and a high-protein corn product was formulated (16.9 mega joule/kg gross energy and 12.7 g/kg lysine). Two levels of crystalline L-isoleucine (0 or 1.0 g/kg), two levels of crystalline L-valine (0 or 1.0 g/kg), and two levels of crystalline L-tryptophan (0 or 0.5 g/kg) were added to the basal diet for a total of 8 diets that were used in a 2 × 2 × 2 factorial. Results indicated that fecal N output increased if isoleucine (Ile) was added to diets without adding valine (Val), but that was not the case if Val was added (interaction, P < 0.05). Isoleucine addition to diets reduced N retention, but N retention increased with tryptophan (Trp) addition to diets without valine addition, but not if Trp was added to diets with added Val (interaction, P < 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P < 0.05). In Exp. 2, a total of 288 growing pigs (body weight: 28.6 ± 2.5 kg) were randomly assigned to 9 dietary treatments in a 28-day growth performance experiment. There were 2 barrows and 2 gilts per pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal (16.5 mega joule/kg gross energy and 11.1 g/kg lysine) was used in Exp. 2 in addition to the 8 diets used in Exp. 1. Results indicated that final body weight and average daily gain of pigs fed the control diet were greater (P < 0.001) than for pigs fed all other diets, except pigs fed the diet with addition of both L-valine and L-tryptophan. Pigs fed the control diet also had greater (P < 0.001) plasma concentrations of Trp and Val than pigs fed the basal diet or diets not containing L-tryptophan or L-valine, respectively. In conclusion, adding Ile alone reduced N retention, but adding Trp alone or in combination with Ile or Val increased N retention. The combination of Val and Trp supplementation may be beneficial for preventing detrimental effects of excess Leu on growth performance of pigs.

Influence of Guar Meal from Pig Compound Feed on Productive Performance, Nitrogen Metabolism, and Greenhouse Gas Emissions

Guar (Cyamopsis tetragonoloba) is an annual legume tolerant to drought. Guar meal (GM) is a protein- and carbohydrate-rich co-product generated after the mechanical separation of the endosperm from the germ and hull of guar seed. GM has received considerable interest in animal feed as an alternative to soybean meal (SM). In this study, we aimed to assess the nitrogen (N) balance indicators, performance, carcass traits, and main greenhouse gas (GHG) emissions resulting from enteric fermentation (E-CH4) and manure (M-CH4 and N2O). Two tests were performed: (i) a biological trial on 45 pigs (15 animals/group) and (ii) a digestibility test in metabolism cages (N = 15, 5 replicates/group). Three different diets were given to the pigs: one diet was based on 0% GM (SM diet); in the second, GM-50%, GM replaced 50% of the SM; and the third was GM-100%, in which GM fully replaced the SM. The GM and SM diets were analyzed for their proximate composition. A model based on prediction equations was used to estimate the GHGs. GM up to 10% in the diets of finishing pigs did not significantly impact growth performance or carcass traits, although a slight increase in neutral detergent fiber (NDF) was observed. GM up to 10% improved N digestibility (p &lt; 0.0001), net protein utilization (p &lt; 0.0001), the biological value of protein, coefficients of metabolizability, and the coefficient of the total tract’s apparent digestibility. Irrespective of its dietary proportion, GM decreased total nitrogen output (TNO, p = 0.11). A highly significant impact was noted for N2O and E-CH4 (for DM, p &lt; 0.0001), as well as a significant impact for E-CH4, expressed as g CO2 Eq (p = 0.007), and g CO2 Eq. LU (livestock unit, p = 0.005), also reported as ADG (p = 0.024). Manure, M-CH4, was not significantly influenced. In conclusion, GM can replace up to 100% SM and is thus a valuable byproduct that does not alter animal performance and can positively impact N2O and E-CH4.

Expanding the range of flour confectionery products for specialized nutrition

Currently, the range of flour confectionery products for nursing women is not large, although this group of the population needs specialized food products. The aim of the work is to develop a functional sugar cookie using non-traditional types of flour for feeding nursing women. The object of study was a traditional sugar cookie. It was decided to improve the biological and nutritional value by adding non-traditional flour varieties to the recipe. Pumpkin seed flour and soy flour are rich in essential amino acids and minerals. Experimental samples were baked with different dosages of pumpkin and soy flour in the composition. By the method of organoleptic tasting, a sample with a ratio of wheat, soy and pumpkin flour, respectively, 70:20:10, was selected for further research. To improve the flavor characteristics of the product, it was decided to add a liquid extract of roasted chicory. When using 100 g of sugar cookies, the daily energy needs are covered by 15%. The needs for carbohydrates are covered by 10%, in fats by 25%, and in proteins by 12%. Cookies contain a large amount of vitamin E 22% and vitamin K 25% of the daily value. Trace elements are also contained in sufficient quantities: 34% copper, 29% manganese, 20% magnesium, 19% phosphorus. The coefficient of biological value of proteins is higher than that of traditional sugar cookies. The biological efficiency of lipids is quite high. The indicators for PUFA are high, however, the score for NLC and oleic acid is close to the "ideal lipid".

Сезонные изменения биологической ценности белков молока ослиц киргизской породы

Due to its protein component, donkey’s milk is a multifunctional product with a wide range of beneficial physiological properties. It is known for its antioxidant, anti-inflammatory, rejuvenating, antimicrobial, and antitumor effects. However, the milk of Kyrgyz donkeys has not received proper scientific attention in this respect. This study analyzed the seasonal amino acid profile and biological value of milk obtained from Kyrgyz donkeys.&#x0D; The donkeys belonged to the Kyrgyz breed and inhabited the highlands of the Chui Region, Republic of Kyrgyzstan. The sampling took place in April, July, and September. The amino acid composition of milk proteins was determined by capillary electrophoresis. The biological value of proteins was assessed by amino acid score, index of essential amino acids (Auxerre index), utility ratio of essential amino acids, and utility ratio of amino acid composition.&#x0D; The analysis revealed 18 amino acids, including eight essential ones. The total content of amino acids in the spring samples was 1840.07 mg/100 g. In the summer samples, this value peaked at 2037 mg/100 g; in autumn, it dropped to 1264.35 mg/100 g. The changes could be explained by the seasonal differences in the diet. The ratio of essential to non-essential amino acids approached 1:1. The milk also proved to contain arginine, which is an essential amino acid for a juvenile organism. In the spring samples, its content was 2.5 times as big as in the autumn ones. Leucine and isoleucine were responsible for 1/3 of the essential amino acids (322.60 ± 19.35 mg/100 g). The summer samples demonstrated the maximal content of some other essential amino acids, except for valine and tryptophan, which appeared to be the limiting essential amino acids for donkey’s milk in the autumn period. Total essential amino acids in the spring, summer, and autumn samples amounted to 54.24, 59.26, and 34.77 g/100 g, respectively, which exceeded the value for the reference protein (27.46 g/100 g).&#x0D; Kyrgyz donkey’s milk proteins were of high biological value, regardless of the season, which means that donkey’s milk can become part of new functional products.

Regulating the composition and secondary structure of wheat protein through canopy shading to improve dough performance and nutritional index

Viscoelastic properties of gluten proteins critically determine the biscuit-making quality. However, cultivar genetics and light conditions closely regulate the composition of the gluten proteins. The impact of pre- and post-anthesis shading (60 %) on amino acid profile, gluten protein composition, secondary structure, dough performance, and biscuit-making quality were evaluated using four wheat cultivars that differ in gluten protein composition. Pre- and post-anthesis shading increased the contents of gliadin, by 35.8 and 3.1 %; glutenin, by 27.6 and 7.3 %; and total protein, by 21.7 and 10.6 %, respectively, compared with those of unshaded plants. Conversely, the ratios of glutenin/gliadin, ω-/(α,β + γ)-gliadin, and high-molecular-weight/low-molecular-weight glutenin subunits decreased with shading. Strong-gluten cultivars exhibited smaller declines in these parameters than weak-gluten cultivars. Secondary structure analysis of the wheat protein revealed that shading increased β-sheet content but decreased β-turn content. Changes in protein components and their secondary structures caused an increase in wet gluten content, dough development time, and gluten performance index, thereby decreasing the biscuit spread ratio. Shading stress increased the protein content and nutrition index but decreased the biological value of protein by 2.5 %. Transcriptomic results revealed that shading induced 139 differentially expressed genes that decreased carbohydrate metabolism and increased amino acid metabolism, involved in increased protein content. Thus, canopy shading improves dough performance and nutrition index by regulating the amino acid profiles, protein compositions, and secondary structures. The study provides key insights for achieving superior grain quality under global dimming.

Amino acid score of the most consumed foods and relationship of the different meal times with the nutritional status of ESPOCH students 2020

Introduction: The quality of food must be diverse, balanced, healthy, which provides energy and nutrients necessary for the perfect functioning of the organism. Objective: To describe the relationship of the main meal times with the nutritional status of university students and to calculate the amino acid score in foods most consumed in the restaurants of the Higher Polytechnic School of Chimborazo-Riobamba-Ecuador. Methodology: Descriptive cross-sectional study with the application of a semi-structured survey on the frequency of eating times for breakfast, lunch, snack and types of preparations with the highest consumption applied to 483 university students who used ESPOCH bars, with voluntary participation. The statistical analysis was carried out with the free version InfoStat/L 2020 program, where it was found that more than 50% are within normal parameters. Results: Regarding the frequency of consumption of the different meal times according to nutritional status, 48.2% had breakfast daily, 59.8% had lunch and 56.3% had a snack. Of the foods with the highest consumption, two limiting amino acids, threonine and tryptophan, are evident due to the consumption of unhealthy foods with a lower biological value of proteins. It is recommended to make suitable combinations to achieve the balance of essential amino acids.

Biological value of zaanen goats' pasteurized milk proteins

Goat's milk is positioned as biologically complete, suitable for the creation of functional products and baby food products. This is explained by the qualitative and quantitative composition of its main nutrients: proteins, lipids, carbohydrates, biologically active substances, micro- and macro-elements. The biological value of food raw materials is assessed by its ability to satisfy protein needs. Strict thermal treatment regimens are used for goat milk. This is caused by the quantitative ratio of casein and albumin fractions in goat milk and its specific organoleptic properties. Pasteurization of milk causes partial destruction of proteins, enzymes, hormones and evaporation of gaseous. This contributes to the improvement of sensory properties of dairy goat raw materials. The effect of high temperatures on goat milk proteins and its biological value has been little studied. The effect of pasteurization regimes on proteins was studied and indicators of the biological value of the samples were determined: without heat treatment; heat treatment 63 ± 2 °С, duration 30 minutes; heat treatment 87 ± 2 °С duration 5-6 minutes. The amino acid composition of the test samples was determined by acid hydrolysis on an LC2000 amino acid analyzer (Biotronik, Germany). Indicators of the biological value of proteins of pasteurized goat milk were calculated – coefficient of difference of amino acid composition, biological value, coefficient of utilitarian amino acid composition, coefficient of rationality of amino acid composition, coefficient of comparative redundancy. The general analysis of the obtained data revealed a positive effect of heat treatment on the indicators of the biological value of goat milk. The amino acid score of the limiting amino acid increased by 14.82–14.92 %; biological value – 11.4–13.02 %; the PDCAAS indicator – by 14.18–14.29 %; changes in the values of formalized indicators had the same tendency. The biological value of proteins for the application of thermal regimes is at the same level. It has been proven that pasteurization has a positive effect on the biological value of goat milk proteins. This makes the product safe and useful for all segments of the population and can be recommended for feeding children from 0 to 6 months. Prospects for further research are the development of milk drinks of a combined composition of raw materials with an improved recipe in order to enrich the product with limiting amino acids.

Digestibility and quality of edible insect proteins: a systematic review ofin vivo studies

Edible insects are being considered as a potential sustainable food source to address global food security concerns and feed the world’s growing population. However, the quality and biological protein value of insects remain uncertain. This review analysedin vivo studies that evaluated edible insect consumption to clarify their potential as a high-quality protein source. Ten studies that evaluated protein quality in rats or mice were included, and most studies found weight gain similar to or greater than the control group. Food intake, net protein ratio, and biological value were also similar to those in the control group. However, true digestibility and protein efficiency ratio were generally lower in animals fed with insects, and higher nitrogen excretion was observed. The results of this systematic review were highly heterogeneous, and it cannot be concluded that insects have high protein quality, although positive results regarding weight gain and biological value were found.

Rheological and physicochemical properties of Spirulina platensis residues-based inks for extrusion 3D food printing

Novel food matrices (such as microalgae, plants, fungi, and microbial proteins) with high protein content and biological value, good amino acid profile, and functionality have been explored. Phycocyanin and active polysaccharides extracted from Spirulina platensis are used as food additives, treatment of colitis, as well as obesity prevention. However, most of the remaining Spirulina platensis residues are mainly used as fish feed at present. 3D food printing is one of the promising development techniques used in the food industry. The aim of this study was to develop a novel 3D printing material of Spirulina platensis residues with shear thinning characteristics, high viscosity and rapid recovery. The effects of moisture content and pretreatment method on the rheological properties of Spirulina platensis residues were clarified. Scanning electron microscopy was used to observe the microstructure and texture profile analysis was used to determine the texture characteristics of Spirulina platensis residues, rheology was used to determine the key 3D printing factors such as viscosity and modulus of Spirulina platensis residues. More importantly, the printing process could be realized under ambient conditions. The development of microalgae residue ink promoted the high-value and comprehensive utilization of microalgae, and also broadened the application of microalgae in the food field.

Combined Effect of Acid Whey Addition and Ultrasonic Treatment on the Chemical and Microbiological Stability of Lamb Stuffing.

The microbiological and chemical stability of stuffing is crucial in meat processing. Small ruminant (lamb) meat has many nutritional advantages (fatty acid composition and ratio, high biological value of protein, source of zinc, and selenium) but is poorly utilized in processing. In this experiment, we determined the effects of physical (sonication), chemical (salt, curing salt, and air access), and microbiological (acid whey) factors on the microbiological and chemical stability of lamb meat stuffing. Proximate composition and fatty acids profile, pH, water activity, lipid oxidation, color parameters, and microbiology examinations were performed in fresh meat stuffing and on the fifth day of refrigerated storage. Ultrasound treatment of the meat with whey reduced its acidity and increased the oxidative stability of fats but did not modify the water activity and color of the stuffing. Stuffing sonication did not affect the growth of LAB but reduced the number of Entereobacteriace, especially in the presence of whey and salt. The treatment of lamb meat stuffing with low-frequency and medium-intensity ultrasound assisted by the addition of acid whey and salt is a technique conducive to reducing the use of nitrates in meat technology and their consumption by consumers.

Effect of Pectin Structure on Protein Utilization by Growing Rats

The effect of pectins' molecular weight (MW) and degree of esterification (DE) on apparent digestibility (D), net protein utilization (NPU) and biological value (BV) of casein were tested. The pectins prepared differed in MW and DE respectively as follows: P-1 (180,000, 73%); P-2 (300,000, 28%); P-3 (60,000, 74%) and P-4 (75,000, 40%) and were fed as 10% of a purified diet containing 10% casein to growing male rats for 10 days. All preparations decreased D, with P-2 and P-4 showing milder effects than P-1 or P-3. Increased NPU was observed with pectins of low MW and/or DE, being significant only with P-4. Diets containing P-2, P-3 or P-4 had higher BV for casein than the pectin-free diet. Feeding these pectins in a protein-free diet did not cause a significant change in the rats' body N content. Thus, the increase in the BV of casein was attributed to improvements in the pattern of amino acids absorbed by rats fed pectins with low MW and/or low DE.pectins pectin structure protein utilization digestibility casein biological value

Effect of Production Technology Intensity on the Grain Yield, Protein Content and Amino Acid Profile in Common and Durum Wheat Grain

Products derived from wheat grains are an important source of protein in the daily diet of people in many parts of the world. The biological value of protein is determined by its amino acid composition and the proportions of the individual amino acids. Synthesis of these compounds in wheat grains is influenced by genetic factors, as well as habitat conditions and the agrotechnology applied in cultivation. The aim of this study was to assess the effect of production technology (integrated, intensive) on the grain yield and the content amino acid profile of protein in common and durum wheat grain. Field research was conducted at the Experimental Station IUNG-PIB in Osiny (Poland) in two growing seasons. It was found that grain yield significantly depended on the weather conditions in the years of harvesting and genotype, but did not depend on the production technology. On the other hand, the protein content and their amino acid composition depended significantly on the production technology and genotype. A significantly higher content of protein substances was found in durum wheat grain. Increasing the intensity of production technology had a positive effect on the total protein content and the content of individual amino acids, both exogenous and endogenous. The amino acid limiting the biological value of protein contained in grains of both wheat species was lysine, and the deficiency of this amino acid was significantly lower in grain protein from intensive than integrated cultivation technology.