Biological Objects Research Articles

OPTIMIZATION OF RESOURCE ALLOCATION, EXPOSURE TIME AND ROTARY SPEED OF INCUBATIVE EGGS

Recently, the laser technology of influencing biological objects in biology, medicine, and veterinary medicine has become widespread in order to activate certain biochemical and physiological processes in the organism. Any influence of electromagnetic radiation (in particular optical emission) requires the exact adherence to the recommended illumination dose to obtain a positive effect on the biological object. The article presents the results of a theoretical study concerning provision of uniform illumination of the egg’s surface, taking into account the location of the laser radiation source and rotating time of the egg.

Biology Learning Innovation in Environmental Change Materials: Approach and Method

The object of learning biology is the universe and its contents. However, not all of these objects can be brought to school. One of the biological materials with these characteristics is environmental change material which is taught in class X SMA. On the other hand, teachers have attempted to design strategies to maximize learning, starting from approaches, models, methods, and media. Through biology learning innovations that integrate technology can facilitate more effective and enjoyable learning. The contextual approach with the field study method is very suitable to support learning about environmental change. Therefore, an innovation in biology learning was carried out on environmental change material. This study uses a qualitative descriptive method with data collection techniques, namely literature studies from articles from various journals, websites, and books that are relevant to the topics discussed. The results of this study are recommendations for innovative field study methods and contextual approaches in learning about environmental change with the help of technology. The use of these methods and approaches uses Google Earth technology, Class Tools, IoT, Source Net + HP Camera, Google Spreadsheets, Macroinvertebrates, LiveGap Charts, and Instagram which are expected to produce more meaningful learning.

In English

Nowadays, people are constantly under the influence of electromagnetic radiation, which can cause health deterioration. The creation of ceramic materials that protect biological and technical objects from the negative effects of electromagnetic radiation is relevant for Ukraine and the world. The purpose of the study is to create composite ceramics with the addition of graphite and conduct experimental studies of the electrophysical properties of such material samples in the frequency range of 26–37.5 GHz. The results of experimental studies of the developed ceramics based on facing tiles with an electrically conductive additive of 10, 20, and 30 % wt. of graphite. To determine the parameters of the interaction of electromagnetic radiation with the samples, the modernized standard equipment – P2-65 microwave standing wave coefficient and attenuation meter, was used. The phase composition of the material was determined using the method of X-ray phase analysis using a DRON-3M diffractometer with CuKα radiation with a nickel filter. The developed composite ceramic materials meet the basic requirements for the operation of similar materials and can be used to weaken the high-frequency electromagnetic field inside premises located in the areas of radio radiation action, and for environmental purposes to reduce the intensity of the electromagnetic field outside the premises where sources of radio radiation are present. Thus, the developed composite ceramics have characteristics that allow them to be used in construction and in electronic devices for the purpose of effective shielding of harmful radio radiation, and the developed ceramics, according to the classification, can be classified as radio-absorbing.

ANALYSIS OF DIELECTRIC PROPERTIES OF HUMAN TISSUES: RESULTS OF EX VIVO EXPERIMENTS

The aim of the work was to evaluate the dielectric properties of human tissues in ex vivo conditions. The study was performed on 68 tissue samples, 20 of which were fragments of muscle tissue, 17 – fatty, 11 – fibrous and 20 – a full-layer skin flap. The analysis of the dielectric properties of tissue fragments was carried out by the method of near-field resonant microwave sounding using a special software and hardware complex. The study made it possible to verify the presence of pronounced differences in the dielectric properties of tissues depending on their histotype. It was revealed that adipose tissue has the highest level of dielectric permittivity, and fibrous tissue has the lowest one. Muscle tissue and a full-layered skin flap occupy an intermediate position between them. These data are fundamentally important for the creation of standards for the dielectric characteristics of biological objects and the further development of near-field resonant microwave sensing technology as an innovative medical imaging technology.

Biocompatible Phosphorescent O2 Sensors Based on Ir(III) Complexes for In Vivo Hypoxia Imaging.

In this work, we obtained three new phosphorescent iridium complexes (Ir1-Ir3) of general stoichiometry [Ir(N^C)2(N^N)]Cl decorated with oligo(ethylene glycol) fragments to make them water-soluble and biocompatible, as well as to protect them from aggregation with biomolecules such as albumin. The major photophysical characteristics of these phosphorescent complexes are determined by the nature of two cyclometallating ligands (N^C) based on 2-pyridine-benzothiophene, since quantum chemical calculations revealed that the electronic transitions responsible for the excitation and emission are localized mainly at these fragments. However, the use of various diimine ligands (N^N) proved to affect the quantum yield of phosphorescence and allowed for changing the complexes' sensitivity to oxygen, due to the variations in the steric accessibility of the chromophore center for O2 molecules. It was also found that the N^N ligands made it possible to tune the biocompatibility of the resulting compounds. The wavelengths of the Ir1-Ir3 emission maxima fell in the range of 630-650 nm, the quantum yields reached 17% (Ir1) in a deaerated solution, and sensitivity to molecular oxygen, estimated as the ratio of emission lifetime in deaerated and aerated water solutions, displayed the highest value, 8.2, for Ir1. The obtained complexes featured low toxicity, good water solubility and the absence of a significant effect of biological environment components on the parameters of their emission. Of the studied compounds, Ir1 and Ir2 were chosen for in vitro and in vivo biological experiments to estimate oxygen concentration in cell lines and tumors. These sensors have demonstrated their effectiveness for mapping the distribution of oxygen and for monitoring hypoxia in the biological objects studied.

A Landmark-free Approach for Surface Asymmetry Detection and Profile Drawings from Bilaterally Symmetrical Geometry

Bilaterally symmetrical objects represent a large and important proportion of archaeological artifacts and biological objects. The identification of the plane of symmetry plays a vital role in quantifying surface asymmetry and producing profile drawings in archaeology and anthropology. The correct recognition of symmetry provides evidence to allow experts to restore damaged artifacts, assess consistency in artifact manufacture, and examine morphological variability in human development. With the increasing availability of archaeological and anthropological three-dimensional (3D) meshes, landmark-based and landmark-free morphometric methods for detecting planes of symmetry have both been proposed. However, the landmark-based approach requires manual identification of landmark locations, and hence they are time-consuming and prone to error. Additionally, the landmark-independent morphometric method is influenced by missing data. This study presents an effective landmark-free approach to approximate the best-fitted plane of symmetry from nearly bilaterally symmetrical objects by means of finding the plane with the minimum geometric differences between the original and mirrored meshes. Subsequently, a global and regional method is carried out to quantify surface asymmetry, reducing the effect of the size and orientation of 3D meshes on gross asymmetry detection. Finally, profile drawings are produced by computing the intersections of the plane of symmetry and 3D meshes. Both synthetic and real objects are used to evaluate the effectiveness and robustness of the proposed method. Our results show the approximated plane of symmetry generated by the proposed method is consistent with that determined by anatomical landmarks, and no significant differences in asymmetry ratio representing the degree of gross asymmetry are found between the landmark-based and proposed methods. These results demonstrate that the proposed method provides a suitable plane of symmetry from a bilaterally symmetrical object with small geometric distortion or simple missing geometry, thereby speeding up asymmetry detection and profile drawings.

Investigation of the Antifungal and Anticancer Effects of the Novel Synthesized Thiazolidinedione by Ion-Conductance Microscopy

In connection with the emergence of new pathogenic strains of Candida, the search for more effective antifungal drugs becomes a challenge. Part of the preclinical trials of such drugs can be carried out using the innovative ion-conductance microscopy (ICM) method, whose unique characteristics make it possible to study the biophysical characteristics of biological objects with high accuracy and low invasiveness. We conducted a study of a novel synthesized thiazolidinedione’s antimicrobial (for Candida spp.) and anticancer properties (on samples of the human prostate cell line PC3), and its drug toxicity (on a sample of the human kidney cell line HEK293). We used a scanning ion-conductance microscope (SICM) to obtain the topography and mechanical properties of cells and an amperometric method using Pt-nanoelectrodes to register reactive oxygen species (ROS) expression. All data and results are obtained and presented for the first time.

Simulation of a proton beam facility in the TOPAS MC software package

The aim of this work is to simulate the formation system of therapeutic proton beams at the accelerator of the Institute of Nuclear Research, Russian Academy of Sciences, using the TOPAS MC software package and to calculate, on this basis, the characteristics of irradiation of biological objects. As such characteristics, the dependence of the absorbed dose and linear energy transfer (LET) on the proton range in water and the effective dose of irradiation of HT-29 and HCT-116 cell lines used in real experiments are considered. Also, the uniformity of irradiation with a high dose rate of the studied biological objects was evaluated during the operation of the radiation unit in FLASH-therapy modes. The research is aimed at preparing and conducting radiobiological experiments with proton beams with a record dose rate.

Development of an Optical System with an Orientation Module to Detect Surface Damage to Potato Tubers

This method is a variant of non-destructive multiparametric surface analysis and includes the implementation of hyperspectral and RGB image processing approaches from different angles. This work is based on a fundamental hyperspectral survey system for obtaining data on scanned biological objects in many spectral ranges and with several possible variants of assembling a system with different types of surface illumination with point light and diffuse illumination. The implementation of the technology through the use of a diffused light source provides the diffuse illumination of a biological object with pronounced symptoms of rarefaction on the surface of a biological object—in this case, potato tubers, due to the presence of signs of disease on the potato peel, as well as their deformation. Using broadband lamps, a short-pass filter is located between the source and the object (λ ≤ 400 nm, λ may vary depending on the excitation length), and a long-pass filter (λ ≥ 400 nm) between the root or onion and the chamber. The use of a vision system with a created database containing models of real defects in potato tubers showed a high sorting efficiency, providing an accuracy of sorting by size of 95.4%, and an accuracy by the presence of defects of 93.1%.

A microcomputed tomographic analysis of the morphological variabilities and incidence of extra canals in mandibular first molar teeth in an Egyptian subpopulation

A well-protected microbial habitat may be present in the root and canal morphology, which is varied and complicated. Before initiating effective root canal treatment, a detailed knowledge of the root and canal anatomical variances in each tooth is a must. This study aimed to investigate the root canal configuration, apical constriction anatomy, location of the apical foramen, dentine thickness, and prevalence of accessory canals in mandibular molar teeth in an Egyptian subpopulation using micro-computed tomography (microCT). A total of 96 mandibular first molars were scanned using microCT, and 3D reconstruction was performed using Mimics software. The root canal configurations of each of the mesial and distal root were classified with two different classification systems. The prevalence and dentin thickness around middle mesial and middle distal canals were investigated. The number, location and anatomy of major apical foramina and the apical constriction anatomy analysed. The number and location of accessory canals were identified. Our findings showed that two separate canals (15%) and one single canal (65%) were the most common configuration in the mesial and distal roots, respectively. More than half of the mesial roots had complex canal configurations and 51% had middle mesial canals. The single apical constriction anatomy was the most common for both canals followed by the parallel anatomy. Disto-lingual and distal locations of the apical foramen are the most common location for both roots. Mandibular molars in Egyptians show a wide range of variations in root canal anatomy with high prevalence of middle mesial canals. Clinicians should be aware of such anatomical variations for successful root canal treatment procedures. A specific access refinement protocol and appropriate shaping parameters should be designated for each case to fulfil the mechanical and biological objectives of root canal treatment without compromising the longevity of treated teeth.

The impact of the ecological and climatic characteristics of the territory on the condition of the housing stock

The problem of environmental pollution is relevant at the present time. Any enterprise is a potential source of environmental pollution, and the production of road construction materials is one of the enterprises that pose a significant danger to the environment. As an example, we chose Primasfalt LLC, which is one of the main enterprises serving the roads of the city of Vladivostok. At all stages of the preparation of asphalt concrete mixtures, pollutants are released. Toxic substances accumulate in the atmospheric air, then fall on the ground in the form of liquid and solid precipitation, penetrating into the soil, groundwater is polluted, and the balance of nutrients in the soil is disturbed. In the course of the study, an assessment was made of the impact of asphalt concrete production on the condition of soils through the use of test objects — living organisms that are able to respond to environmental changes. The test object was oats, the most common and widely available bioindicator of soil conditions. Sampling points are located within the sanitary protection zone of the enterprise. When sampling, the climatic characteristics of the city of Vladivostok were taken into account. The results of the study showed that high toxicity of soils is observed at points located in the zone of maximum values of wind speed and its frequency. With the help of bioindicator organisms, it is possible to quickly and inexpensively analyze the state of the natural environment. Assessing the activity of an asphalt concrete plant with the help of biological objects makes it possible to identify the intensity of its impact not only within the framework of normative values that are aimed specifically at humans, but also at biological objects in general, which determines the relevance of the chosen topic. The purpose of the study is to assess the level of soil toxicity within the regulatory sanitary protection zone of the LLC Primasfalt enterprise in the city of Vladivostok, Primorsky Krai.

AOST2031: An in-progress COG phase 3 randomized controlled trial comparing open vs thoracoscopic management of pulmonary metastases in patients with osteosarcoma.

TPS11579 Background: Pulmonary metastasectomy is considered a necessary component of curative therapy in pulmonary metastatic osteosarcoma, however the optimal surgical approach is unknown. Two approaches include open surgery (OSY) including thoracotomy or sternotomy and minimally invasive surgery (MSY) by thoracoscopy. No randomized trial has been completed comparing these approaches in patients with osteosarcoma, however randomized controlled trials in other cancers have not identified superior outcomes in patients undergoing OSY. OSY allows for direct palpation of all lung surfaces and more pulmonary metastatic nodules can be identified during OSY compared to the number predicted by pre-operative imaging. MSY offers the benefit of faster patient recovery, improved quality of life and potential for equivalent surgical control of relevant metastatic disease. Given wide variability in surgical approach and willingness of both surgical oncology and patient advocacy communities to participate in a randomized trial, AOST2031 was developed to address this clinical equipoise. Methods: Eligibility for AOST2031 includes patients < 50 yrs with a histological diagnosis of osteosarcoma and evidence of pulmonary metastases at initial diagnosis or at first recurrence. Patients must have oligometastatic lung metastasis considered resectable by either OSY or MSY, with rapid central review of chest CT images to confirm eligibility. Systemic therapy considered equivalent to methotrexate, cisplatin and doxorubicin is required to be ongoing in newly diagnosed and completed in patients with recurrence. Exclusion criteria include unresectable primary disease, metastatic disease requiring anatomic lung resection, pleural or mediastinal lesions or pleural effusions, progression while receiving initial therapy, extra-pulmonary metastases and prior chest surgery for pulmonary metastases. Patients are randomized 1:1 to either OSY or MSY, with randomization stratified by disease status and risk. The primary outcome is thoracic event free survival, defined as intrathoracic tumor recurrence or death that results from the procedure or a complication related to the procedure. Two-hundred and twenty-five eligible patients are anticipated to answer the primary objective. Secondary objectives include event free, overall survival and . Exploratory objectives include surgical complication rates, patterns of recurrence, use of surgical localization techniques, comparison of CT findings to pathology of resected specimens, and patient reported outcomes. Biologic objectives include the collection of metastatic tissues to facilitate the study of metastatic disease and serial blood samples for future tumor profiling, germline and circulating tumor DNA studies. AOST2031 opened to enrollment at NCTN sites in February 2022. Clinical trial information: NCT05235165 .

Dose-efficient scanning Compton X-ray microscopy

The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 70 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 17 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.

Application of Scanning Electron Microscopy to Study Morphological and Anatomical Features of Sea Buckthorn Leaves

Introduction. Scanning electron microscopy is a modern method that allows us to study not only the morphological features of objects, but also to conduct micro-X-ray structural analysis. Currently, the method is being actively introduced into the study of biological objects (including plant ones). Sea buckthorn (Hippophaë rhamnoides L.) leaves are a non-pharmacopoeial type of medicinal plant raw materials. Further study of the morphology, anatomy and phytochemical composition of leaves can contribute to the production of new, including combined, medicines, which will require the development of a pharmacopoeia article for this medicinal plant raw material.Aim. The aim of the study was to study the morphological and anatomical features of sea buckthorn leaves by scanning electron microscopy.Materials and methods. The object of the study was dried whole leaves of sea buckthorn (Hippophaë rhamnoides L.), collected in the Voronezh region in 2021 during the period of mass fruit maturity. To carry out the study by scanning electron microscopy, pieces of leaves were previously sprayed with gold on an automatic spraying unit Q150R ES (Quorum Technologies Ltd., United Kingdom) to increase conductivity. Micrographs were obtained using an electron microscope JSM-6510LV (JEOL Ltd., Japan).Results and their discussion. The morphology and some features of the anatomical structure of sea buckthorn leaves were studied and the main microdiagnostic signs (surface character, types of trichomes, the presence of stomata) were clarified. The content of elements (silicon, potassium, aluminum, carbon and calcium) was determined during the microrentgenostructural analysis. Micrographs of pollen grains of the plant were obtained, the presence of the element iron in them was established.Conclusion. For the first time, the method of scanning electron microscopy was used to study the morphological and anatomical features of sea buckthorn leaves. The main diagnostic signs of leaves and their location have been clarified. It has been established that carbon predominates in the composition of the raw material elements, and calcium also accumulates. The accumulation of aluminum, silicon and potassium in numerous hairs densely covering the upper, and especially the lower, surfaces of the leaf blade is assumed. The morphology of the surface of pollen grains of sea buckthorn, having a spherical shape with a spiny shell, has been established. Pollen grains, in addition to carbon, are characterized by the accumulation of iron and silicon.

Efficient and green esterification approach for determining of sodium alkylbenzene sulfonates in dry extracts

Linear alkylbenzenesulfonate is an anionic surfactant widely used in detergents and cleaners, both in industrial and household applications as well as a component of pesticides and fertilizers. In this work, the universal effective green approach for sodium alkylbenzenesulfonate esterification to determine these substances in a form of their volatile derivatives by gas chromatography coupled with electron ionization mass-spectrometry (GC–MS) is presented. The approach provides a simple conversion of sodium alkylbenzene sulfonates to a form of alkylbenzenesulfonic acid methyl esters in 98% yield for 20 min at 24 °C. The yield was estimated using high performance liquid chromatography-ultraviolet detection. Trimethyl orthoformate (125 µl) was used as low toxicity alkylation reagent. The obtained volatile derivatives might be redissolved in n-hexane and analyzed by GC–MS in selective ion monitoring mode. The limit of quantification of 20 alkylbenzenesulfonate isomer sum was estimated as 25 ng μL–1 of the analyzed extract. The approach might be applied to food, environmental and biological objects contaminated with alkylbenzene sulfonates. The applicability of the method is shown using dry solvent-free extracts of snow, river sediments, freshwater branching sponge Lubomirskia baikalensis, the whole blood, and widely cultivated plants such as spinach Spinacia oleracea and cucumber Cucumis sativus. The presence of sodium alkylbenzene sulfonates in snow (31.1 ± 1.0 µg L–1) and sediments (208±7 µg g–1 of d.w.) of the world's deepest Lake Baikal tributaries as well as in freshwater sponge (33.6 ± 1.1 µg g–1 of d.w.) was found. The repeatability of measurement is characterized by RSD = 2.24%.

Evaluation of the Influence of Super-Low-Intensity Microwave Radiations on Mesenchimal Stem Cells

This paper is devoted to the analysis of advanced scientific research on the role of super-low-intensity microwave effects on biological objects in form of stem cells. The scientific work has been performed by a translational team of researchers, including doctors, engineers, radio physicists, and programmers. Synchropulsar AQUA was used as a source of super-low intensity <10 μW of electromagnetic microwave radiation in the 1 GHz band. During the study, it was found that the number of cells is reduced in the experimental groups compared with the control. In the control it was calculated 3.1 x106 cells/mL, in the low frequency biomodulation mode it was found 2.5×106 cells/mL; in the continuous generation mode it was revealed 2.1 x106 cells/mL. Assessment of the fluorescence intensity showed that the activity of mitochondria is higher in the experimental group under irradiation with mode modulation by biotropic parameters – 12.40 conventional units (cu). When using regime continuous generation, the activity of mitochondria was 9.33 cu. In the control group, mitochondrial activity was 10.74 cu.

Intracavity enhancement of GFP fluorescence induced by femtosecond laser pulses

The phenomenon of fluorescence is widely used in molecular biology for studying the interaction of light with biological objects. In this article, we present an experimental investigation of the enhancement of laser-induced fluorescence of Clytia gregaria green fluorescent protein. The laser-induced fluorescence method applied in our work combines the advantages of femtosecond laser pulses and a photonic crystal cavity, with the time dependence of the fluorescence signal studied. It is shown that a green fluorescent protein solution placed in a microcavity and excited by femtosecond laser pulses leads to an increase in fluorescence on the microcavity modes, which can be estimated by two orders of magnitude. The dependences of fluorescence signal saturation on the average integrated optical pump power are demonstrated and analyzed. The results obtained are of interest for the development of potential applications of biophotonics and extension of convenient methods of laser-induced fluorescence.

Profile of Science Generic Skills in Biology Learning Materials for Students in Class X

Generic Science Skills (GSS) are the ability to think and act based on the knowledge possessed by individuals. Every human being has generic science skills that need to be developed along with the development of the human brain. This is because generic science skills can be developed in accordance with the development of the level of human thinking. After the COVID-19 pandemic, teaching and learning activities experienced changes in the learning process. This resulted in learning originally carried out in the network (online) turning into offline learning (offline). Biology is a science that allows students to discover for themselves the concepts and facts contained in the biology material. Finding concepts in biology requires direct observation of biological objects. This study aimed to describe generic science skills among class X students at SMAN 1 Daha Utara. The research was conducted using a descriptive method. The description of students' generic science skills was obtained from filling out the questionnaire distributed to students. The generic science skills questionnaire used uses aspects that refer to Brotosiswoyo in 2001, which contain direct observation, indirect observation, awareness of scales, symbolic language, a framework of mind obeying the principles of natural law, logical inference, the law of cause and effect, mathematical modeling, and building the concept. This study took the population of all class X students of SMAN 1 Daha Utara. The sample for this study took 98 students randomly from six classes, namely classes X1-X6. This study uses data analysis techniques that are carried out by categorizing test results based on low, medium, and high categories. Based on the results of this study, it appears that students' generic science skills are included in the high category.

Automated extraction of seed morphological traits from images

Abstract The description of biological objects, such as seeds, mainly relies on manual measurements of few characteristics, and on visual classification of structures, both of which can be subjective, error prone and time‐consuming. Image analysis tools offer means to address these shortcomings, but we currently lack a method capable of automatically handling seeds from different taxa with varying morphological attributes and obtaining interpretable results. Here, we provide a simple image acquisition and processing protocol and introduce Traitor, an open‐source software available as a command‐line interface (CLI), which automates the extraction of seed morphological traits from images. The workflow for trait extraction consists of scanning seeds against a high‐contrast background, correcting image colours, and analysing images with the software. Traitor is capable of processing hundreds of images of varied taxa simultaneously with just three commands, and without a need for training, manual fine‐tuning or thresholding. The software automatically detects each object in the image and extracts size measurements, traditional morphometric descriptors widely used by scientists and practitioners, standardised shape coordinates, and colorimetric measurements. The method was tested on a dataset comprising of 91,667 images of seeds from 1228 taxa. Traitor's extracted average length and width values closely matched the average manual measurements obtained from the same collection (concordance correlation coefficient of 0.98). Further, we used a large image dataset to demonstrate how Traitor's output can be used to obtain representative seed colours for taxa, determine the phylogenetic signal of seed colour, and build objective classification categories for shape with high levels of visual interpretability. Our approach increases productivity and allows for large‐scale analyses that would otherwise be unfeasible. Traitor enables the acquisition of data that are readily comparable across different taxa, opening new avenues to explore functional relevance of morphological traits and to advance on new tools for seed identification.

Transparent Organic and Metal Halide Tandem Scintillators for High-Resolution Dual-Energy X-ray Imaging

Dual-energy X-ray imaging (DEXI) is a cutting-edge technology that provides more detailed material-specific information than the traditional single-energy X-ray imaging strategy. Herein, we designed and fabricated a top-filter-bottom (TFB) sandwich structure scintillator for high-resolution DEXI within a single exposure. More specifically, the low- and high-energy X-ray photons were sequentially absorbed by the top and bottom scintillators and were efficiently converted into their corresponding emission colors. By discriminating between these different emission spectra of the transparent TFB sandwich structure scintillator, DEXI can provide not only unique X-ray energy information but also an exceptional resolution of approximately 18 lp/mm on stacked images that surpasses most of the reported single-layer organic- and metal halide-based scintillators. The conceptual demonstrations of decomposition and reconstruction in DEXI were also realized on several biological imaging objects. This breakthrough research paves the way for the development of scintillator architectures specifically designed for DEXI.