Biological Materials Research Articles

Natural product of angelica essential oil developed as a stable Pickering emulsion for joint interface lubrication

Development of high-performance joint injection lubricants has become the focus in the field of osteoarthritis treatment. Herein, natural product of angelica essential oil combined with the graphene oxide were prepared to the stable Pickering emulsion as a biological lubricant. The tribological properties of the Pickering emulsion under different friction conditions were studied. The lubricating mechanism was revealed and the biological activities were evaluated. Results showed that the prepared Pickering emulsion displayed superior lubrication property at the Ti6Al4V biological material interface. The maximum friction reduction and anti-wear abilities of the Pickering emulsion were improved by 36% and 50% compared to water, respectively. This was primarily due to the action of the double-layer lubrication films composed of the graphene oxide and angelica essential oil molecules. It was worth noting that the friction reduction effect of the Pickering emulsion at the natural cartilage interface was higher about 19% than that of HA used in clinic for OA commonly. In addition, the Pickering emulsion also displayed antioxidant activity and cell biocompatibility, showing a good clinical application prospect in the future.

On the impact of radiative transfer in fluorescence imaging of bacterial films and suspensions

Fluorescence imaging and spectroscopy are convenient, rapid, and simple methods to analyze chemical samples including biological materials such as bacterial biofilms and suspensions. In principle, these techniques could be used to diagnose or discriminate between infectious bacteria in infections of the skin or ocular surface (microbial keratitis, MK). However, the extension of these techniques to macroscopic turbid media that strongly absorb and scatter light is difficult. Radiative transfer effects obscure the relationship between microscopic scattering and absorption properties and macroscopically observable quantities such as fluorescence intensity, transmission, and reflection. A combination of experimental measurements of aqueous bacteria cell suspensions and Monte Carlo radiation transfer simulations are performed to better understand these effects. Several general observations, e.g., that fluorescence intensity is maximized in scattering-dominated media, are discussed in detail. It was found that wavelength-dependent radiative transfer effects are observable even at moderate optical densities (OD ∼ 1; well below the diffusion limit). Careful consideration of radiative transfer effects using physically rigorous models is needed to determine single-cell scattering and absorption properties and interpret quantitative fluorescence measurements accurately in most cases of interest. A detailed discussion of radiative transfer effects and analytical models is provided. In the context of surface infections and MK, it was found that radiative transfer effects may be negligible for the model bacteria E. coli in some cases. However, more accurate measurements of microbe optical properties are needed to confirm and extend this conclusion to other species. Overall this work demonstrates that quantitative fluorescence imaging and spectroscopy of bacterial films and suspensions is feasible, but requires detailed sample characterization and careful consideration of radiative transfer effects.

Application of Platelet-rich Fibrin as a Method of Treatment for Medication-related Osteonecrosis of the Jaws in Bulgaria

Lately, medication-related osteonecrosis of the jaw (MRONJ) has emerged as a common disease due to the increasing use of antiresorptive drugs (intravenous bisphosphonates) for the treatment of oncological and osteoporotic diseases, which at an advanced stage significantly affects the quality of life of patients. Therefore, new therapies that accelerate the recovery of musculoskeletal tissue have become preferred in the treatment of such diseases in recent years. An innovative approach, which supports the healing processes is the application of autologous platelet concentrates known as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). Platelets are unique blood elements responsible for hemostasis. Activated platelets secrete a large amount of growth factors that initiate regeneration in soft tissues and bones. PRP is plasma with many more platelets than are usually found in the blood. The concentration of platelets and therefore the concentration of growth factors may be 5–10 times higher (or richer) than usual. PRF is a biological material, which has the ability to release growth factors over time. Growth factors stimulate stem cells, which improve collagen and elastin in the skin. Platelets in PRF are thought to survive better than those in PRP and thus contribute to faster recovery and formation of new tissue. Fibrin is a biological scaffold that forms when an injury occurs anywhere in the body. Platelets normally circulate in the blood and bind to the fibrin scaffold. Thus connected, they are activated and release growth factors, which are small “granules”. These growth factors begin the process of wound healing by creating new skin cells, collagen and blood vessels. The present study is related to the treatment of MRONJ with PRF. It is aimed at researching the attitudes of oral and maxillofacial surgeons in Bulgaria regarding the application of innovative medical technology such as PRF in the treatment of MRONJ. An author's questionnaire was developed with the help of which the survey was carried out. The analysis of the data shows that the interest in PRF in Bulgaria is great, but unfortunately it is rarely applied. However, respondents believe that this is a promising and innovative method in various aspects of surgery.

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

AbstractInspired by the powerful biological olfaction, scientists extracted numerous materials such as olfactory sensory neuron, olfactory receptor (OR) protein, and odorant binding protein from animal olfactory systems, then combined them with transducers to form multiple odor biosensors. These biosensors, despite well inheriting the sensing ability of creatures, have several drawbacks, such as complex preparation process, unstable sensing material characteristics, and high cost. Unlike the biological materials listed above, cell expressing heterologous OR maintains a stable sensing performance after passaging for multiple generations, also its experimental operation is simple, and cost is low. Therefore, odor biosensors based on cell expressing OR have been well developed in recent years. In this review, we first listed several odor biosensors based on cell expressing OR, mainly focusing on fluorescent and electrophysiological measurement methods. Furthermore, we illustrated the techniques to improve the biosensor performance, e. g., wider detection range, longer lifetime, more OR types, and higher quantification efficiency. In addition, we explained the possible prospects such as big sensor array and predicting odor response.

Acidentes de trabalho com exposição a material biológico entre profissionais de enfermagem no Paraná

The objective was to analyze the epidemiological profile of notifications of work accidents with exposure to biological material among nursing professionals in Parana, through the historical series determined for the years 2013 to 2022. Descriptive observational epidemiological study of the survey type, based on descriptive epidemiology, based on data from the System Information on Notifiable Diseases and the National Registry of Health Establishments. 48,405 cases were identified, with a prevalence of 98.54/1,000. More occurrences among female nursing technicians aged between 31 and 40 years old, from the Metropolitan Health Regional Facilities, in year 2019, stand out. Among nurses, the age range was between 18 and 30 years old, with discharge progressing without serological conversion and negative sources of disease. The high number of abandoned treatments and underreporting stands out. Investing in professional training, continuing education, quality protective equipment and implementing protocols for prophylactic treatment are essential to prevent the disease.

A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material.

The documentation, preservation and rescue of biological diversity increasingly uses living biological samples. Persistent associations between species, biosamples, such as tissues and cell lines, and the accompanying data are indispensable for using, exchanging and benefiting from these valuable materials. Explicit authentication of such biosamples by assigning unique and robust identifiers is therefore required to allow for unambiguous referencing, avoid identification conflicts and maintain reproducibility in research. A predefined nomenclature based on uniform rules would facilitate this process. However, such a nomenclature is currently lacking for animal biological material. We here present a first, standardized, human-readable nomenclature design, which is sufficient to generate unique and stable identifying names for animal cellular material with a focus on wildlife species. A species-specific human- and machine-readable syntax is included in the proposed standard naming scheme, allowing for the traceability of donated material and cultured cells, as well as data FAIRification. Only when it is consistently applied in the public domain, as publications and inter-institutional samples and data are exchanged, distributed and stored centrally, can the risks of misidentification and loss of traceability be mitigated. This innovative globally applicable identification system provides a standard for a sustainable structure for the long-term storage of animal bio-samples in cryobanks and hence facilitates current as well as future species conservation and biomedical research.

Brittle and ductile yielding in soft materials

Many soft materials yield under mechanical loading, but how this transition from solid-like behavior to liquid-like behavior occurs can vary significantly. Understanding the physics of yielding is of great interest for the behavior of biological, environmental, and industrial materials, including those used as inks in additive manufacturing and muds and soils. For some materials, the yielding transition is gradual, while others yield abruptly. We refer to these behaviors as being ductile and brittle. The key rheological signatures of brittle yielding include a stress overshoot in steady-shear-startup tests and a steep increase in the loss modulus during oscillatory amplitude sweeps. In this work, we show how this spectrum of yielding behaviors may be accounted for in a continuum model for yield stress materials by introducing a parameter we call the brittility factor. Physically, an increased brittility decreases the contribution of recoverable deformation to plastic deformation, which impacts the rate at which yielding occurs. The model predictions are successfully compared to results of different rheological protocols from a number of real yield stress fluids with different microstructures, indicating the general applicability of the phenomenon of brittility. Our study shows that the brittility of soft materials plays a critical role in determining the rate of the yielding transition and provides a simple tool for understanding its effects under various loading conditions.

Succession changes of microbial community for inferring the time since deposition of saliva.

Saliva is a common biological examination material at crime scenes and has high application value in forensic case investigations. It can reflect the suspect's time of crime at the scene and provide evidence of the suspect's criminal facts. Even though many researchers have proposed their experimental protocols for estimating the time since deposition (TsD) of saliva, there is still a relative lack of research on the use of microorganisms to estimate TsD. In the current study, the succession change of microbial community in saliva with different TsD values was explored to discern the microbial markers related to TsD of saliva. We gathered saliva samples from six unrelated healthy Han individuals living in Guizhou, China and exposed these samples to indoor conditions at six time points (0, 1, 3, 7, 15, and 28 days). Temporal changes of microbial compositions in these samples were investigated by 16S rRNA sequencing (V3-V4 regions). By assessing temporal variation patterns of microbial abundance at the genus level, four bacteria (Brucella, Prevotella, Pseudomonas, and Fusobacterium) were observed to show good time dependence in these samples. In addition, the hierarchical clustering and principal co-ordinates analysis results revealed that these saliva samples could be classified into t-short (≤7 days) and t-long (>7 days) groups. In the end, the random forest model was developed to predict the TsD of these samples. For the model, the root mean square error, R2, and mean absolute error between predicted and actual TsD values were 1.5213, 0.9851, and 1.1969, respectively. To sum up, we identified TsD-related microbial markers in saliva samples, which could be viewed as valuable markers for inferring the TsD of saliva.

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review.

Skin tissue engineering (STE) is widely regarded as an effective approach for skin regeneration. Several synthetic biomaterials utilized for STE have demonstrated favorable fibrillar characteristics, facilitating the regeneration of skin tissue at the site of injury, yet they have exhibited a lack of in situ degradation. Various types of skin regenerative materials, such as hydrogels, nanofiber scaffolds, and 3D-printing composite scaffolds, have recently emerged for use in STE. Electrospun nanofiber scaffolds possess distinct advantages, such as their wide availability, similarity to natural structures, and notable tissue regenerative capabilities, which have garnered the attention of researchers. Hence, electrospun nanofiber scaffolds may serve as innovative biological materials possessing the necessary characteristics and potential for use in tissue engineering. Recent research has demonstrated the potential of electrospun nanofiber scaffolds to facilitate regeneration of skin tissues. Nevertheless, there is a need to enhance the rapid degradation and limited mechanical properties of electrospun nanofiber scaffolds in order to strengthen their effectiveness in soft tissue engineering applications in clinical settings. This Review centers on advanced research into electrospun nanofiber scaffolds, encompassing preparation methods, materials, fundamental research, and preclinical applications in the field of science, technology, and engineering. The existing challenges and prospects of electrospun nanofiber scaffolds in STE are also addressed.

MONITORING OF PESTICIDE RESIDUES IN GRAPEVINE LEAVES UNDER VARIANT FUNGAL DISEASE MANAGEMENT

Food contamination is the result of over-reliance on fungicides in crop production. The presence of pesticide residues in agricultural commodities needs to be strategically solved by applying gentle agrotechnical procedures. The research aimed to evaluate the effect of the treatment of grapevine with fungicides on the content and mobility of 4 active substances in the leaves of the variety Hibernal. The experiment included a control variant treated with fungicides and an untreated variant. We focused on monitoring the active substances of fungicides used in the first half of the vegetation: azoxystrobin, cyflufenamid, difenoconazole, myclobutanil, and another 25 active substances, in terms of multi-residue analysis methodologies. Grape leaves for analysis were collected on two dates: in the phenological phase of berry development BBCH 73 and 14 days after grape harvest – BBCH 91. Samples were prepared according to STN EN 15662. For qualitative analyzes of residues, we used the QuEChERS method in combination with liquid (LC-MS/MS) and gas (GC-MS/MS) chromatography with tandem mass spectrometry. In the untreated variant, the contents of residues from both leaves collection were in undetectable values. In the leaf samples of the treated variant collected in BBCH 73, we established higher values of residues than the MRL determines, namely for azoxystrobin 0.083 mg/kg (MRL – 0.01 mg/kg). Azoxystrobin is the active substance of the Quadris max fungicide, and we applied this preparation 41 days before the first collection of leaves. In the analyzes of leaf sampling from the treated variant after grape harvest in BBCH 91, we found a decrease in the content of all monitored residues below detectable values. We consider the gradual degradation of residues in biological materials to be a very favorable result from the point of view of their impact on consumer health and the environment. Through visual observations of the vegetation, we found out that even the untreated variant had a perfect state of health. Hibernal is a variety suitable for biological production systems, and we assume that even in vintages with high pressure of fungal diseases, it is possible to maintain the health of the grapevine with ecological products. Using the appropriate grape variety combined with the right agrotechnical interventions makes it possible to use safer ecological practices to produce healthy food, maintaining sustainability and a healthy natural environment. The research will continue in the early Table grape varieties, where the vegetation period is shorter, and space for breaking down the residues is more difficult.

The wildcat (Felis s. silvestris) in the Mediterranean forest: sighting through photo-trapping and non-invasive hair collection for genetic purposes.

The European wildcat (Felis silvestris silvestris) is a mesocarnivore species widely distributed in Europe, from Eastern Europe to Portugal and from Scotland to Italy. Recent biogeographical studies of wildcat populations have endeavoured to assess in detail the various issues that pose a threat to this species, including hybridization with domestic cats. The use of non-invasive sampling methods supported by photo-trapping and some attractants has made it possible to gather genetic material for the detection of native wildcats in locally threatened populations, some of which live in the Iberian Peninsula. Testimonies of naturalists, hunters and farm workers led our team to choose specific areas in two large territories of Mediterranean forests where the presence of wildcats has been historically attested: the Almonte River basin and the Sierra de San Pedro Mountains. Between 2014 and 2018, non-invasive hair sampling was performed using valerian (Valeriana officinalis) as an attractant and supported by photo-trapping to guarantee the collection of genuine biological material (hair samples). The hair samples were genetically assessed by sequencing the nuclear gene IRBP (interphotoreceptor retinoid-binding protein) and the mtDNA gene ND4 (NADH dehydrogenase subunit 4). Despite the low density of wildcats, this combined protocol proved to be an applicable tool for detecting the presence of elusive wildcats and other mesocarnivore species in this remote region of southern Europe. In addition, non-invasive hair trapping contributes to the collection of genetic material from current wildcat populations. This procedure could enhance future management actions focused on collecting quality individualized biological material.

Geometric modeling of phase ordering for the isotropic–smectic A phase transition

BackgroundLiquid crystal (LC) mesophases have an orientational and positional order that can be found in both synthetic and biological materials. These orders are maintained until some parameter, mainly the temperature or concentration, is changed, inducing a phase transition. Among these transitions, a special sequence of mesophases has been observed, in which priority is given to the direct smectic liquid crystal transition. The description of these transitions is carried out using the Landau–de Gennes (LdG) model, which correlates the free energy of the system with the orientational and positional order.MethodologyThis work explored the direct isotropic-to-smectic A transition studying the free energy landscape constructed with the LdG model and its relation to three curve families: (I) level-set curves, steepest descent, and critical points; (II) lines of curvature (LOC) and geodesics, which are directly connected to the principal curvatures; and (III) the Casorati curvature and shape coefficient that describe the local surface geometries resemblance (sphere, cylinder, and saddle).ResultsThe experimental data on 12-cyanobiphenyl were used to study the three curve families. The presence of unstable nematic and metastable plastic crystal information was found to add information to the already developed smectic A phase diagram. The lines of curvature and geodesics were calculated and laid out on the energy landscape, which highlighted the energetic pathways connecting critical points. The Casorati curvature and shape coefficient were computed, and in addition to the previous family, they framed a geometric region that describes the phase transition zone.Conclusion and significanceA direct link between the energy landscape’s topological geometry, phase transitions, and relevant critical points was established. The shape coefficient delineates a stability zone in which the phase transition develops. The methodology significantly reduces the impact of unknown parametric data. Symmetry breaking with two order parameters (OPs) may lead to novel phase transformation kinetics and droplets with partially ordered surface structures.

ВОЛНОВОДНО ДИЭЛЕКТРИЧЕСКИЙ МЕТОД И УСТРОЙСТВА ДЛЯ ИЗМЕРЕНИЯ КОНЦЕНТРАЦИИ ВОДНЫХ РАСТВОРОВ И ОБЛУЧЕНИЯ БИОЛОГИЧЕСКИХ ОБЪЕКТОВ

A technique for studying the absorption of microwave radiation of biological objects in a thin dielectric capillary is proposed. The results of experimental studies are presented and the mechanism of the detected effects of the biological action of microwave radiation is proposed. It was found that at a certain ratio between the dimensions of the waveguide, the diameter and thickness of the walls of the capillary, the dielectric parameters of the biological material that fills the capillary and the wavelength of microwave radiation in the waveguide, a significant increase in the absorbed power in a rather narrow frequency band (waveguide-dielectric resonance) is possible. This phenomenon must be taken into account in biotechnological experiments on the irradiation of suspensions. As a result of the work done on the study of the absorption of microwave radiation by hydrocarbon mixtures and models of biological objects in aquatic environments, new designs of measuring devices were created and a technique for hydrocarbon aquametry was developed. The created products are protected by the authors of this work by a patent for an invention.

Implementation of bio-inspired organic/inorganic layer structures as interphase in carbon fiber reinforced concrete

Biological materials found in nacre or glass sponges reveal specific layered organic and inorganic structures known for their fracture toughness caused by crack deflection and bridging. This work aims to bio-mimic the brick-and-mortar (BnM) structure from nacre and the layer-by-layer (LbL) structure from the glass sponge filaments to incorporate these effects into the contact zone of carbon fiber-reinforced concrete (CFRC) in order to prevent brittle composite failure. To build up BnM- and LbL-structure materials such as nanoclays, sodium water glass, and polymer dispersions were selected since they are well-established low-cost materials in building. Nanoclays were analyzed regarding their size, dispersibility and exfoliation. Montmorillonite (MMT) was used to be mixed with polymers to produce self-assembled BnM-structured films and coatings. Also, LbL-structures were formed by alternating layers of sodium water glass and polymer. Scanning electron microscopy and energy-dispersive X-ray spectroscopy were used to verify the morphology. The MMT-containing coatings demonstrated enhanced nucleation potential when exposed to cementitious eluate. Micromechanical pull-out tests on single carbon fibers with BnM- and LbL-coatings embedded in concrete demonstrate the potential to increase composite toughness. The successful implementation of the bio-inspired structures using affordable materials lays the groundwork for their scalability and integration into composite structures for building.

Puerarin-containing rhein-crosslinked tyramine-modified hyaluronic acid hydrogel for antibacterial and anti-inflammatory wound dressings

Wound infections, posing a grave risk of severe physical consequences and even mortality, exact a substantial financial toll on society, rendering them among the most formidable challenges confronting our world today. A critical imperative is the development of hydrogel dressings endowed with immune-regulating and antibacterial properties. This study is founded upon the symbiotic physical and efficacious attributes of two small natural molecules. An injectable hydrogel is meticulously crafted by encapsulating puerarin (PUE) into tyramine-modified hyaluronic acid, subsequently introducing rhein (RHE), and catalyzing the formation of inter-phenol crosslinks with H2O2/horseradish peroxidase (HA-Tyr-R@P). Exhibiting a favorable microenvironmental impact the developed hydrogel attains an antibacterial efficacy exceeding 95 %, coupled with a wound closure rate twice that of the control group. HA-Tyr-R@P hydrogels not only inhibit bacterial growth but also mitigate inflammation, fostering wound healing, owing to their harmonized physicochemical characteristics and synergistic therapeutic effects. This work underscores the creation of a singular, versatile hydrogel platform, negating the complexities and side effects associated with pharmaceutical preparations. Furthermore, it offers new ideas for the formulation of RHE-based hydrogels for wound healing, emphasizing the pivotal role of natural small molecules in advancing biological materials.

Optimizing functionally graded tibial components for total knee replacements: a finite element analysis and multi-objective optimization study

The optimal design of complex engineering systems requires tracing precise mathematical modeling of the system’s behavior as a function of a set of design variables to achieve the desired design. Despite the success of current tibial components of knee implants, the limited lifespan remains the main concern of these complex systems. The mismatch between the properties of engineered biomaterials and those of biological materials leads to inadequate bonding with bone and the stress-shielding effect. Exploiting a functionally graded material for the stem of the tibial component of knee implants is attractive because the properties can be designed to vary in a certain pattern, meeting the desired requirements at different regions of the knee joint system. Therefore, in this study, a Ti6Al4V/Hydroxyapatite functionally graded stem with a laminated structure underwent simulation-based multi-objective design optimization for a tibial component of the knee implant. Employing finite element analysis and response surface methodology, three material design variables (stem’s central diameter, gradient factor, and number of layers) were optimized for seven objective functions related to stress-shielding and micro-motion (including Maximum stress on the cancellous bone, maximum and mean stresses on predefined paths, the standard deviation of mean stress on paths, maximum and mean micro-motions at the bone-implant interface and the standard deviation of mean micro-motion). Then, the optimized functionally graded stem with 6 layers, a central diameter of 5.59 mm, and a gradient factor of 1.31, was compared with a Ti6Al4V stem for various responses. In stress analysis, the optimal stem demonstrated a 1.92% improvement in cancellous bone stress while it had no considerable influence on the maximum, mean, and standard deviation of stresses on paths. In micro-motion analysis, the maximum, mean, and standard deviation of mean micro-motion at the interface were enhanced by 24.31%, 39.53%, and 19.77%, respectively.

Adaptive SARIMA modelling for continuous chamber temperature tracking in ultra-low temperature freezers

Ultra-low temperature (ULT) freezers are vital for storing perishable biological materials, requiring continuous monitoring of chamber thermal conditions to ensure sample integrity. A reliable dynamic data-driven model is key for smart proactive surveillance of the ULT freezers through real-time status tracking. However, the dynamic nature of ULT freezers, influenced by external disturbances, demands adaptive models to sustain performance. Complex models thus face challenges due to time-consuming parameter updates. To ensure model prediction performance while minimising model updating time, this study proposes a Seasonal Autoregressive Integrated Moving Average (SARIMA) model-based approach for short-term ULT freezer chamber temperature prediction. This method solely relies on historical chamber temperature data, eliminating the need for additional measurements. Moreover, two adaptive duty cycle length (DCL) selection strategies and a one-step ahead DCL estimation method are developed to address auto-correlation issues caused by recurring duty cycles. Comparative analyses with reference models demonstrate that the SARIMA model, incorporating future DCL effects, excels in one-step prediction and multi-step forecast under stable and variant DCL periods. Moreover, it outperforms the reference models in auto-correlation representation, affirming its reliability in predicting future states. The proposed modelling approach promises an effective method for freezing chamber temperature prediction, enabling continuous status tracking and early fault detection. Moreover, it holds great potential for modelling other critical temperatures in refrigeration systems and facilitating flexible energy operations.

A device for rapid calorimetric measurements on small biological tissue samples

AbstractA new calorimetric technique is described that allows high-throughput heat production rate measurements on small biological tissue samples. The technique is based on the widely used thermopile chip technology combined with an innovative method for precise transport and positioning of samples of different biological materials at the thermal power detector inside the calorimeter. The new transport and positioning technique is a combination of fluidic and mechanical transport, where the latter is realized by a magneto-motor drive. The transport facility ensures good diffusive oxygen penetration into the sample, which is essential for highly metabolically active materials. The proper functioning of the device is demonstrated by measuring the heat production of metabolically active brown adipose tissue, biopsied tegu lizard muscle, and live Drosophila larvae at different stages and temperatures.

Genetic tests as the strongest motivator of cooperation between participants and biobanks-Findings from cross-sectional study.

The development of the scientific potential linked with biobanking and research on human biological material is highly dependent on the willingness of potential donors to cooperate with entities that collect the material. For this reason, it is crucial to identify the circumstances and factors that may encourage potential participants to donate their biological material. In particular, knowledge of the motivational factors that can be modified by the persons managing a biobank may prove notably important for shaping the organizational and communication policy of the biobank and other scientific institutions. The research was carried out on a group of 1,100 people over 18 years of age representing the adult population of Poland in 2021. More than half of the respondents declared their willingness to donate a blood sample for research purposes to a biobank (57.8%). The most often indicated incentives among the factors supporting the donation of biological material were offers of: obtaining the results of genetic tests predicting the risk of diseases (77.1%), blood tests (71.3%), the possibility of obtaining a small remuneration (64.6%) and the carrying out of genetic ancestry tests (60.4%). Offering the possibility of performing additional diagnostic tests, especially genetic tests, may significantly increase the willingness of potential donors to cooperate with biobanks and other entities collecting human biological material for the purpose of scientific research. However, attention should also be paid to the challenges and risks linked with respecting the privacy and autonomy of research participants.

Contrasting the validation parameters and greenness of normal-phase and reverse-phase stability-indicating HPTLC methods for lemborexant analysis

There are very limited analytical procedures to determine lemborexant (LMB) in dosage forms and biological materials. However, the literature does not provide any “high-performance thin-layer chromatographic (HPTLC)” assays to determine LMB. In an effort to analyze LMB in commercial pharmaceutical tablets more precisely, accurately, and sustainably than with the normal-phase-HPTLC (NP-HPTLC) approach, a sensitive and greener reverse-phase-HPTLC (RP-HPTLC) method was developed and validated. For NP-HPTLC, an acetone-petroleum ether (40:60 v/v) developing system was used. However, ethanol–water (85:15 v/v) was the developing system for RP-HPTLC. Four different techniques, including the National Environmental Method Index (NEMI), Analytical Eco-Scale (AES), ChlorTox, and Analytical GREENness (AGREE), were used to evaluate the greenness of both procedures. LMB measurement was linear in the 50–500 and 20–1000 ng/band ranges, respectively for NP and RP procedures. RP procedure was more robust (uncertainties = 0.90–0.95 %), accurate (recoveries = 98.24–101.57 %), precise (uncertainties = 0.87–1.00 %), linear (20–1000 ng/band), sensitive (LOD = 0.92 ng/band and LOQ = 2.76 ng/band), and greener over NP procedure. The results of greenness assessment using NEMI (all four circles green), AES (93), ChlorTox (0.88 g), and AGREE (0.89) demonstrated that the RP strategy was greener than NP strategy and all other reported HPLC methods. The fact that both techniques can assess LMB when its degradation products are present implies that they both have characteristics that point to stability-indicating features. 89.24 % and 98.79 %, respectively, were the assay results for LMB in pharmaceutical tablets when utilizing the NP and RP procedures. Based on all validation and greenness metrics, it was found that RP procedure was better than the NP procedure. As a result, it is possible to assess LMB in pharmaceutical products using RP procedure.