Biological Characteristics Research Articles

Biological characteristics of Moolgarda cunnesius (Valenciennes, 1836) in Thua Thien Hue, Vietnam

Moolgarda cunnesius (Valenciennes, 1836) is a species of the Mugilidae family, commonly known as a delicious fish with high nutritional and economic value, making it very promising. Zoological research methods and DNA barcoding are used to study this fish. Morphological Characteristics: The Leaf mullet has 62 to 75 gill rakers on the lower limb of the first gill arch. The upper jaw is slightly curved downward, and the anterior margin of the anterior alveolar is slightly concave. The body has 37 to 43 scales. The first dorsal fin has 4 spines, while the second dorsal fin is smaller and starts behind the anal fin. Pectoral Fins: These fins are 2/3 of the head length. Anal and Pelvic Fins: These fins are small. The second dorsal and anal fins have few scales, with scales only on the anterior and basal parts. The dorsal surface is green, the flanks and abdomen are iridescent silver, and the fins are slightly dull. There is a difference in the measurement criteria between the study areas, but there is no difference between male and female groups. Mahalanobis distance analysis showed no conformational difference between male and female groups of M. cunnesius and no similarity between sexes. Nutritional biological characteristics: The food composition includes a variety of zooplankton phytoplankton benthic animals, and organic detritus. Organic humus has the highest occurrence, followed by dinoflagellates, blue-green algae, diatoms, and the lowest is arthropods. This diverse food composition illustrates that M. cunnesius is an omnivorous species. Genetic Characteristics: COI gene DNA barcoding was used to study the genetic diversity of M. cunnesius in two regions of the North and the South of Tam Giang lagoon. Amplified and sequenced 10 samples of the COI gene of M. cunnesius in Tam Giang lagoon recorded a size of 650 bp. The rate of difference in COI sequences of the 2 samples is low compared to previous studies. The similarity coefficient of M. cunnesius in the two areas of Tam Giang lagoon is very high (0.9953).

Survey of Fungal Diseases on Barley, Wheat, and Oats at Tillering to Stem Extension Stages in Southern Regions of Korea during 2020-2021

Cereal, including barley, wheat, and oats, is a major winter food crop in Korea. Despite recent changes in agricultural environments in response to climate change, fungal diseases that could affect cereal productivity remain poorly understood. In this study, we investigated the incidence of diseases in barley, wheat, and oats in the southern part of Korea. We collected fungal pathogens from seven locations where cereals were grown. In March-April of 2020 and 2021, a total of 92 fungal isolates were collected, mainly from the stem base or leaves of cereal crops during the tillering and stem extension stages of cereals in Korea. The collected isolates were identified based on morphological and molecular biological characteristics. The dominant species was <i>Ceratobasidium cereale</i> (42.4%), followed by <i>Pyrenophora teres</i> (21.7%), <i>P. avenae</i> (10.9%), <i>Alternaria alternata</i> (6.5%), and <i>Epicoccum tobaicum</i> (6.5%). In addition, <i>P. tritici-repentis</i> (3.3%), <i>Cladosporium</i> sp. (3.3%), <i>Fusarium</i> sp. (3.3%), and <i>Nigrospora</i> sp. (2.2%) were also collected as minority groups. Our results will provide information on fungal pathogens that occur during the growing season of cereals in Korea, particularly during the tillering and stem extension stages. In addition, the isolates collected from this study can serve as a valuable resource for conducting simulations on climate change, focusing on temperature and humidity.

Single-cell and spatial omics unravel the spatiotemporal biology of tumour border invasion and haematogenous metastasis.

Solid tumours exhibit a well-defined architecture, comprising a differentiated core and a dynamic border that interfaces with the surrounding tissue. This border, characterised by distinct cellular morphology and molecular composition, serves as a critical determinant of the tumour's invasive behaviour. Notably, the invasive border of the primary tumour represents the principal site for intravasation of metastatic cells. These cells, known as circulating tumour cells (CTCs), function as 'seeds' for distant dissemination and display remarkable heterogeneity. Advancements in spatial sequencing technology are progressively unveiling the spatial biological features of tumours. However, systematic investigations specifically targeting the characteristics of the tumour border remain scarce. In this comprehensive review, we illuminate key biological insights along the tumour body-border-haematogenous metastasis axis over the past five years. We delineate the distinctive landscape of tumour invasion boundaries and delve into the intricate heterogeneity and phenotype of CTCs, which orchestrate haematogenous metastasis. These insights have the potential to explain the basis of tumour invasion and distant metastasis, offering new perspectives for the development of more complex and precise clinical interventions and treatments.

Integrated analysis to identify biological features and molecular markers of poorly cohesive gastric carcinoma (PCC)

As one of the two main histologic subtypes of gastric cancer (GC), diffuse-type gastric cancer (DGC) containing poorly cohesive gastric carcinoma (PCC) components has a worse prognosis and does not respond well to typical therapies. Despite the large number of studies revealing the complex pathogenic network of DGC, the molecular heterogeneity of DGC is still not fully understood. We obtained single-cell RNA-seq data and bulk data from the tumor immune single cell hub, the public gene expression omnibus, and the cancer genome atlas databases. A series of bioinformatics analyses were performed using R software. Immunofluorescence staining, hematoxylin and eosin staining, western blot, and functional experiments were used for experimental validation. Caudin-3, -4 and -7 were lowly expressed in DGC and their expression levels were further reduced in PCC. The PCC components were mainly located in the deeper layers of the DGC and had a high level of hypoxic Wnt/β-catenin signaling and stemness. We further identified Insulin Like Growth Factor Binding Protein 7 (IGFBP7) as a marker for PCC components in the deep layer. IGFBP7 is stimulated by hypoxia and promotes cancer cell invasiveness and reduced claudin expression. In addition, programmed death-1 ligand (PD-L1) was specifically expressed in the deep layer, reflecting deep layer-specific immunosuppression. The PCC components are predominantly situated in the deeper layers of DGC. Initial molecular characterization of these PCC components revealed distinct features, including low expression of claudin-3, -4, and -7, high expression of IGFBP7, and the presence of PD-L1. These molecular traits may partially account for the pronounced tumor heterogeneity observed in GC.

Glutamate Transport Proteins and Metabolic Enzymes are Poor Prognostic Factors in Invasive Lobular Carcinoma.

Invasive Lobular Carcinoma (ILC) is a subtype of breast cancer characterized by distinct biological features, and limited glucose uptake coupled with increased reliance on amino acid and lipid metabolism. Our prior studies highlight the importance of glutamate as a key regulator of ILC tumor growth and therapeutic response. Here we examine the expression of four key proteins involved in glutamate transport and metabolism - SLC3A2, SLC7A11, GPX4, and GLUD1/2 - in a racially diverse cohort of 72 estrogen receptor-positive (ER+) ILC and 50 ER+ invasive ductal carcinoma, no special type (IDC/NST) patients with primary disease. All four proteins are associated with increased tumor size in ILC, but not IDC/NST, with SLC3A2 also specifically linked to shorter overall survival and the presence of comorbidities in ILC. Notably, GLUD1/2 expression is associated with ER expression in ILC, and is most strongly associated with increased tumor size and stage in Black women with ILC from our cohort and TCGA. We further explore the effects of GLUD1 inhibition in endocrine therapy-resistant ILC cells using the small-molecule inhibitor R162, which reduces ER protein levels, increases reactive oxygen species, and inhibits oxidative phosphorylation. These findings highlight a potentially important role for glutamate metabolism in ILC, particularly for Black women, and position several of these glutamate-handling proteins as potential targets for therapeutic intervention in ILC.

Targeting GPRC5D for multiple myeloma therapy.

Given its nearly ubiquitous expression on plasma cells and limited expression on essential normal tissue, the G protein-coupled receptor class C group 5 member D (GPRC5D) presents a promising opportunity for utilization as an immunotherapy target in multiple myeloma (MM). The therapeutic strategies targeting GPRC5D, such as bispecific antibodies (BsAbs), chimeric antigen receptor (CAR) T cells, and antibody-drug conjugates (ADCs), have been prominently emphasized in relapsed/refractory MM (R/R MM) in recent years. Further clinical trials are necessary to confirm the long-term efficacy of GPRC5D-targeting immunotherapies alone, explore their potentials co-targeting with other specific antigens, or investigate their combinations with existing treatments to overcome MM resistance. This review provides an overview of current research progress in GPRC5D, encompassing its biological characteristics and translational journey from laboratory to clinical application.

Comparative analysis of fisheries and biological characteristics of bream (Abramis brama) in Saratov and Volgograd reservoirs

Based on the results of ichthyological surveys and industrial catch data, a comparative analysis of the commercial biological characteristics of bream in the Saratov and Volgograd reservoirs was carried out. The material was the results of expeditionary research by employees of the Saratov branch of the VNIRO. With the existing fishery, bream stocks are in a stable condition. The volumes of commercial stocks and catches of the species in question in the Volgograd Reservoir are higher than in the Saratov Reservoir. Despite differences in fishing intensity and characteristics of reservoirs, the size and weight indicators of the species are similar.

18F-FDG PET/CT biomarkers as predictors of long term outcomes and survival rates in patients with high risk malignant pulmonary masses/nodules treated with stereotactic ablative radiotherapy.

Stereotactic ablative body radiotherapy (SABR) is a standard treatment option for patients with malignant pulmonary masses (including primary and metastatic lesions) who are unfit for surgery or who are medically operable but refuse surgery. Flourine-18 flurodeoxyglucose positron emission tomography (18F-FDG PET) volumetric metabolic parameters, i.e., metabolic tumour volume (MTV) and total lesion glycolysis (TLG) play an important role in assessing the biological characteristics of some tumours and its role as potential prognostic factors has also been introduced. The aim of this retrospective study is to assess the value of baseline metabolic volumetric parameters as prognostic imaging biomarkers in patients with pulmonary masses/nodules treated with SABR. 70 patients were included in this retrospective study (39 male and 31 female, age range 47-91years, mean 76years). Standardized uptake value (SUVmax), SUVmean, MTV and TLG for all the patients were calculated on baseline 18F-FDG PET/CT. Patient outcome was divided into 3 categories free of disease, stable disease and disease progression. There was no significant statistical difference in the SUVmax and SUVmean in all the three categories. Mean SUVmax ranges from 7.13 to 8.08 with its highest value in the stable disease and lowest value in the progressive disease categories. Similarly, the average SUVmean was 4.9 in the free of disease category and 4.68 in the progressive disease category. MTV and TLG were low in the free of disease and the highest in progressive disease. MTV increased from 2.25 cm3 in free of disease category to 3.23 cm3 and 7.29 cm3 in stable disease and progressive disease, respectively. TLG has increased from 11.7 in the disease-free survival category to 18.77 and 40.39 in the stable and progressive disease, respectively. Patients with low MTV had longer overall survival (OS) than patients with high MTV (37months versus 27months, p value = 0. 0018). In addition, OS was longer in patients with low TLG (36months versus 24months, p value = 0.016). TLG and MTV are more useful than SUVmax and SUVmean for predicting outcome, OS and progression-free survival (PFS) in patients receiving SABR. The TLG and MTV measurement on 18F-FDG PET imaging may be routinely recommended in baseline 18F-FDG PET/CT prior to SABR.

Theoretical and experimental investigation of mixed-ligand metal(II) Schiff base complexes using maleic acid as the auxiliary ligand

This work is focused on the synthesis of several transition metal complexes [ML(MA)], where M = Copper (II), Zinc (II), Cobalt (II) and Nickel (II), MA = maleic acid and L = Schiff base generated from benzene-1,2-diamine [o-phenylenediamine] and 4-chlorobenzaldehyde. The characterization using Fourier-Transform Infrared, Nuclear Magnetic Resonance spectroscopy, Ultraviolet-Visible spectra, Mass, Electro Paramagnetic Resonance and elemental analysis confirm the square planar geometry of the complexes. The in vitro antimicrobial potential of the complexes has been tested by the broth dilution method and the antioxidant method has been done by free radical scavenging analysis. The in vitro methods reveal the outstanding biological characteristics of the copper complexes. The molecular structure of the ligand and its metal (II) complexes has been optimized using Density Functional Theory studies performed by the Gaussian-09 software and their parameters have been discussed. Natural Bond Orbital and Frontier Molecular Orbital analyses have assessed the presence of a metal-ligand bond in complexes. In addition, molecular docking studies have also been performed on antiviral activity of all the complexes using a viral protein and their interacting amino acids.

Fisheries and biological characteristics and state of stocks of the main commercial fish species in Beloye Lake

The current composition of the ichthyofauna and the importance for fisheries of the main fish species in Beloye Lake (Vologda Region) is described in the article based on research materials from 2018 – 2022. The biological features and commercial use of twelve most valuable fish species are summarized as result of the composition of industrial and research catches studies. The size and age composition of catches, the age of puberty, the timing and conditions of spawning, and the feeding patterns of fishes were studied. The influence of net fishing gear with different mesh spacing on commercial fish populations and the magnitude of nets fishing productivity (fishing effort) were studied for the first time. The role of the most important fish species for industrial fisheries in Beloye Lake was assessed.

De novo genome of thick-tailed pygmy jerboa Salpingotus crassicauda, Cardiocraniinae, Dipodidae

Pygmy jerboas are one of the smallest taxa of rodents. They exhibit distinctly different morphological and biological characteristics from other subfamilies, such as more restricted distribution, species richness, reproductive ability, and population size. Agricultural expansion and the development of new energy projects in recent years lead to sharp decline of their natural populations. Here, we assembled and annotated the first reference genome for the subfamily Cardiocraniinae using Illunima and Nanopore sequencing from the thick-tailed pygmy jerboa, Salpingotus crassicauda. The final genome is 2.44 Gb in size, with a contig N50 length of 13.71 Mb and a BUSCO completeness of 96.35%. A total of 23,344 protein-coding genes were annotated in the final genome. We also determined the mitochondrial genome of this species and annotated 13 protein-coding genes, 22 tRNAs, and 2 rRNA. These genomic assemblies provide resources in studying phylogeny and adaptive evolution of Dipodidae, as well as implementing conservation management of jerboas.

Bulk RNA Barcode Sequencing Reveals Role of RNA Splicing in Aging Dermal Stem Cell Modulation by a Botanical Extract

Skin aging is a complex, multifaceted process influenced by both intrinsic and extrinsic factors. Understanding the molecular mechanisms underlying skin aging is crucial for developing effective anti-aging strategies. Dermal stem cells play a pivotal role in maintaining skin homeostasis, but their functionality is compromised with aging. This study investigated the impact of aging on dermal stem cells and explored the potential of natural extracts in modulating their biological characteristics. Using bulk RNA barcoding and sequencing (BRB-seq), we identified differentially expressed genes (DEGs) between young and aged dermal stem cells, revealing alterations in cellular processes, including cell proliferation, ECM synthesis, and RNA splicing. We also demonstrated that a natural extract, comprising callus cells and Alpine rose leaf extracts, influenced RNA splicing in aged dermal stem cells, leading to improved dermal structure and integrity in vitro. Our findings suggest that natural extracts may exert their effects through senolytic activity and the modulation of RNA splicing, a process crucial to gene expression and cellular function. This study underscores the potential of integrating high-throughput transcriptomics in understanding skin aging, presenting new avenues for the development of innovative, sustainable, and effective anti-aging strategies.

Comparative evaluation of the biological characteristics of flower organs and fruits of the new Chinese blueberry cultivar Zhaixuan 4

Comparative evaluation of the biological characteristics of flower organs and fruits of the new Chinese blueberry cultivar Zhaixuan 4

Evaluation of fatty acid composition and antimicrobial activity of supercritical fluid extract of Rubus ulmifolius Schott fruits

In this study, we continue our research on Rubus ulmifolius Schott extracts obtained by supercritical fluid extraction (SFE) using CO2 as solvent. Extraction experiments were carried out at pressures of 300 bar and a temperature of 40 °C, SFE [300:40], on mature fruits of R. ulmifolius. The fatty acid (FA) profile of extracts was assessed by reversed-phase HPLC-DAD/ELSD analysis. The most represented fatty acids in the extract were linoleic acid (386 mg/g), oleic acid (210 mg/g), linolenic acid (112 mg/g), palmitic acid (48 mg/g), and stearic acid (40 mg/g). SFE extraction showed a total FA amount of 795.8 mg/g of dry weight. The chemical and biological characteristics of this extract were compared with those obtained with different extraction methods. Following the diffusion-susceptibility test, three fruit extracts, SFE [300:40], Sx, and MEtOH, didn’t show an inhibition profile against Streptococcus mutans. Kirby-Bauer analysis showed only the methanolic extract demonstrated antimicrobial activity with 11-mm inhibition diameter.

Single-cell RNA sequencing and immune repertoire analysis revealed dynamic immune characteristics associated with peripheral blood during sepsis

Sepsis is a potentially fatal condition arising from an abnormal immune response to an infection, which can result in organ failure and even death. To explore the mechanism underlying the dysregulated immune response during sepsis and identify potential therapeutic targets, single-cell RNA sequencing (scRNA-seq) and immune repertoire analysis were conducted to depict the cellular landscape of peripheral blood cells in septic mice. We observed significant alterations in the number and proportion of peripheral blood cell populations driven by sepsis. By combining single-cell gene expression profiles and B cell receptor (BCR) repertoire analysis, we discerned that infection inflicted serious damage on the antigen presentation ability of B cells and the diversity of BCR in a short time. In addition, we found that the cecal ligation and puncture procedure in mice inhibited the communication signals of CD4+ and CD8+ T cells and decreased the interactions between B cells and other cells. Our study provides detailed insights into the dynamic changes in the biological characteristics of peripheral blood cells driven by sepsis and provides important advances in our understanding of immune disorders during sepsis.

Mechanical and Biological Characterization of Ionic and Photo-Crosslinking Effects on Gelatin-Based Hydrogel for Cartilage Tissue Engineering Applications.

Articular cartilage degeneration poses a significant public health challenge; techniques such as 3D bioprinting are being explored for its regeneration in vitro. Gelatin-based hydrogels represent one of the most promising biopolymers used in cartilage tissue engineering, especially for its collagen composition and tunable mechanical properties. However, there are no standard protocols that define process parameters such as the crosslinking method to apply. To this aim, a reproducible study was conducted for exploring the influence of different crosslinking methods on 3D bioprinted gelatin structures. This study assessed mechanical properties and cell viability in relation to various crosslinking techniques, revealing promising results particularly for dual (photo + ionic) crosslinking methods, which achieved high cell viability and tunable stiffness. These findings offer new insights into the effects of crosslinking methods on 3D bioprinted gelatin for cartilage applications. For example, ionic and photo-crosslinking methods provide softer materials, with photo-crosslinking supporting cell stretching and diffusion, while ionic crosslinking preserves a spherical stem cell morphology. On the other hand, dual crosslinking provides a stiffer, optimized solution for creating stable cartilage-like constructs. The results of this study offer a new perspective on the standardization of gelatin for cartilage bioprinting, bridging the gap between research and clinical applications.

Human Self: Structure of the Recognition-Behaviour Model-System Working with Language-Metabolism and Significance Induced by Symbolical Figuring the Meta-Model-System

Science organizes the recognizable targets of objects and phenomena in the testable form, and it functions to understand, define, quantify, visualize or simulate properties of the target in the syllogistic-hierarchy of framing, modeling, and explanation with sound inferences of language. Human Self maintaining the homeostasis is defined in the thinking-frame. The physical Self is the substantial model-system defined in the physical frame, and the mental Self is the recognition-behavioral model-system defined in the psychological frame. The recognition-behavioral model-system that was analyzed via the scientific procedure, was considered to perform language-metabolism with the thinking-codes of homology-/simplification, clustering, operation and modeling, which are mounted in the Modeling-layer of Neuronal Network of the brain, with economic and stubborn manner coming from the biological characteristics. Systems theory is the transdisciplinary study of systems complex-networked interrelated and interdependent components. Human Self is the meta-model-system complex-networked the functional single-model-systems. The mental Self that was re-defined corresponding to the physical/psychological model-system coming from Chinese philosophy and Chinese traditional medicine, developed to the neuro-psychological Self under the idea of Systems theory. Neuro-psycho-pharmacological effect of the anti-epileptic agents Carbamazepine, Lamotrigine and Valproate on Exceed Stimulation-amplify of patients suffering from Bipolar-disease, which was not clear until recently, was compatibly explained via the neuro-psychological Self. Finally, the emergent neuro-psycho-pharmacological explanation was considered to be induced by symbolically-figuring the neuro-psychological Self in the neuro-psychological frame.

Breast cancer molecular subtype classification according to immunohistochemistry markers and its association with pathological characteristics among women attending tertiary hospitals in Tanzania

BackgroundBreast cancer immunohistochemistry is a biological characteristic of the tumour which has a role to diagnose molecular subtype, prognosticate and guide treatment and is categorised into 4 subtypes. Data in Tanzania was lacking and was based off data extrapolated from studies in Western Africa thus hypothesizing that women of African ancestry predominately develop Triple Negative Breast Cancer (TNBC). MethodsA retrospective cross-sectional study was carried out at two tertiary referral hospitals on participants who were recruited from the cancer registries from 2015 to 2022. Prevalence of each molecular subtype was determined and association between molecular subtype to demographic and pathological characteristics were evaluated. Predictors of molecular subtypes was then determined using logistic regression. ResultsTotal number of participants were 1214, median age was 50 (IQR: 41–61), median tumor size was 5 cm (IQR: 4–7) with lymph node positivity in 73.7 %. Immunohistochemistry studies showed estrogen, progesterone and Human Epidermal Growth Factor Receptor 2 (HER2) receptor positivity in 54.4 %, 34.4 % and 27.8 % of cases respectively. Molecular subtype classification prevalence for Luminal A was 21.17 % (95 % CI: 18.87–23.47), for Luminal B 35.75 % (95 % CI: 33.05–38.45), for HER2 enriched 11.86 % (95 % CI: 10.04–13.68) and for TNBC 31.22 % (95 % CI: 28.61–33.83). Significant association was seen between molecular subtype with age, tumor size, tumor grade and lymph node involvement. Predictors of Luminal tumors were larger tumor size (aOR 1.217, 95 % CI: 1.149–1.291) no lymph node involvement (aOR 0.429, 95 % CI: 0.313–0.589) while an advanced tumor grade reduced likelihood (aOR 0.041, 95 % CI: 0.011–0.019). ConclusionIn Tanzania Luminal B was most predominant subtype presenting at an earlier age and associated with more favorable pathological characteristics.

Biological characterization of lipoic acid- and heme-dependent Escherichia coli small colony variants isolated from sheep in Xinjiang, China.

Escherichia coli (E. coli) small colony variants (SCVs) have garnered attention due to their heightened antibiotic resistance and enhanced cell retention, posing significant risks to public health and food safety. However, understanding of SCVs derived from sheep remains limited. This study aimed to detect the biological characterization of sheep-derived E. coli SCVs and investigate the factors contributing to SCV development with preliminary genomic data. In this study, a lipoic acid-dependent SCV (LA-SCV) and a wild-type (WT) strain were isolated from sheep bile. Then, a heme-dependent SCV (HD-SCV) was induced from WT using amikacin. Initially, we examined factors contributing to SCV formation via comparative genomics. Subsequent comparisons between WT and two SCV strains encompassed antibiotic resistance, hemolytic activity, biofilm formation, motility, and metabolism. Genomic analyses identified a frameshift deletion mutation in the lipA gene in LA-SCV and a stopgain mutation in the hemG gene in HD-SCV, hypothesized as potential triggers for lipoic acid- and heme-dependent SCV development, respectively. Physiological, biochemical, and cultural traits exhibited notable differences between WT and SCVs, including increased antibiotic resistance, hemolytic activity, and biofilm formation, but alongside non-fermentative acetate utilization, slow growth, reduced intracellular ATP, and decreased motility (P < 0.01). The energy and amino acid metabolism were suppressed during the logarithmic phase in LA-SCV, while both logarithmic and stable phases in HD-SCV. These alterations in biological characteristics present significant challenges in managing E. coli pathogenicity and antibiotic resistance.

Single-cell RNA sequencing reveals heterogeneity of ALI model and epithelial cell alterations after exposure to electronic cigarette aerosol

Electronic cigarettes (e-cigarettes) have been advertised as a healthier alternative to traditional cigarettes; however, their exact effects on the bronchial epithelium are poorly understood. Air-liquid interface culture human bronchial epithelium (ALI-HBE) contains various cell types, including basal cell, ciliated cell and secretory cell, providing an in vitro model that simulates the biological characteristics of normal bronchial epithelium. Multiplex single-cell RNA sequencing of ALI-HBE was used to reveal previously unrecognized transcriptional heterogeneity within the human bronchial epithelium and cell type–specific responses to acute exposure to e-cigarette aerosol (e-aerosol) containing distinct components (nicotine and/or flavoring). The findings of our study show that nicotine-containing e-aerosol affected gene expression related to transformed basal cells into secretory cells after acute exposure; inhibition of secretory cell function by down-regulating genes related to epithelial cell differentiation, calcium ion binding, extracellular exosomes, and secreted proteins; and enhanced interaction between secretory cells and other cells. On the other hand, flavoring may alter the growth pattern of epithelial cells and make basal cells more susceptible to SARS-CoV infection. Besides, the data also indicate factors that may promote SARS-CoV-2 infection and suggest therapeutic targets for restoring normal bronchial epithelium function after e-cigarette use. In summary, the current study offered fresh perspectives on alterations in the cellular landscape and cell type–specific responses in human bronchial epithelium that are brought about by e-cigarette use.