By taking the fillings from the wastewater treatment reactor of a practically stable operation of the BAF, it is to test the lipid phosphorus biomass both in the bio-film and floc in the fillings, and active changing laws of microbes. The experiments show that BAF sewage treatment results from the joint action of bio-film and biological floc. The experiments provide theoretical foundations for the operating mechanism of BAF, packing height and the optimization of backwash time. Due to its high efficiency, energy saving, small area, easy operation and management, Biological Aerated Filter (BAF) technology has become a research focus[1] of a biological wastewater treatment technology in recent years. BAF process is characterized of a lot of filler in the activated sludge, besides the bio-film on the fillings. This sludge contains active living organisms, shed bio-film, suspended matters from the raw sewage and other adsorptions from the wastewater. They are collectively referred to as biological floc. When oxidating and decomposing organic matters in the sewage, it intercepts and adsorb sunken substances. Throughout BAF operation process, it is as irreplaceable as the bio-film.