Community Composition Of Biofilms Research Articles

Biofilms on plastic litter in an urban river: Community composition and activity vary by substrate type.

In aquatic ecosystems, plastic litter is a substrate for biofilms. Biofilms on plastic and natural surfaces share similar composition and activity, with some differences due to factors such as porosity. In freshwaters, most studies have examined biofilms on benthic substrates, while little research has compared the activity and composition of biofilms on buoyant plastic and natural surfaces. Additionally, the influence of substrate size and successional stage on biofilm composition has not been commonly assessed. We incubated three plastics of distinct textures that are buoyant in rivers, low-density polyethylene (rigid; 1.7 mm thick), low-density polyethylene film (flexible; 0.0254 mm thick), and foamed polystyrene (brittle; 6.5 mm thick), as well as wood substrates (untreated oak veneer; 0.6 mm thick) in the Chicago River. Each material was incubated at three sizes (1, 7.5, and 15 cm2 ). Substrates were incubated at 2-10 cm depths and removed weekly for 6 weeks. On each substrate we measured chlorophyll concentration, biofilm biomass, respiration, and flux of nitrogen gas. We sequenced 16S and 23S rRNA genes at Weeks 1, 3, and 6 to capture biofilm community composition across successional stages. Chlorophyll, biomass, and N2 flux were similar across substrates, but respiration was greater on wood than plastics. Bacterial and algal richness and diversity were highest on foam and wood compared to polyethylene substrates. Bacterial biofilm community composition was distinct between wood and plastic substrates, while the algal community was distinct on wood and foam, which were different from each other and polyethylene substrates. These results indicate that polymer properties influence biofilm alpha and beta diversity, which may affect transport and distribution of plastic pollution and associated microbes, as well as biogeochemical processes in urban rivers. This study provides valuable insights into the effects of substrate on biofilm characteristics, and the ecological impacts of plastic pollution on urban rivers. PRACTITIONER POINTS: Plastic physical and chemical properties act as forces of selection for biofilm. Biofilm activity was similar among three different types of plastic. Community composition between plastic and wood was different.

Disturbance frequency directs microbial community succession in marine biofilms exposed to shear.

Disturbances are major drivers of community succession in many microbial systems; however, relatively little is known about marine biofilm community succession, especially under antifouling disturbance. Antifouling technologies exert strong local disturbances on marine biofilms, and resulting biomass losses can be accompanied by shifts in biofilm community composition and succession. We address this gap in knowledge by bridging microbial ecology with antifouling technology development. We show that disturbance by shear can strongly alter marine biofilm community succession, acting as a selective filter influenced by frequency of exposure. Examining marine biofilm succession patterns with and without shear revealed stable associations between key prokaryotic and eukaryotic taxa, highlighting the importance of cross-domain assessment in future marine biofilm research. Describing how compounded top-down and bottom-up disturbances shape the succession of marine biofilms is valuable for understanding the assembly and stability of these complex microbial communities and predicting species invasiveness.

Microaerobic methane-driven denitrification in a biotrickle bed – Investigating the active microbial biofilm community composition using RNA-stable isotope probing

A microaerobic (2% O2 v/v) biotrickle bed reactor supplied continuously with 2% methane to drive nitrate removal (MAME-D) was investigated using 16S rDNA and rRNA amplicon sequencing in combination with RNA-stable isotope probing (RNA-SIP) to identify the active microorganisms. Methane removal rates varied from 500 to 1000 mmol m−3h−1 and nitrate removal rates from 25 to 58 mmol m−3h−1 over 55 days of operation. Biofilm samples from the column were incubated in serum bottles supplemented with 13CH4. 16S rDNA analysis indicated a simple community structure in which four taxa accounted for 45% of the total relative abundance (RA). Dominant genera included the methanotroph Methylosinus and known denitrifiers Nubsella and Pseudoxanthomonas; along with a probable denitrifier assigned to the order Obscuribacterales. The 16S rRNA results revealed the methanotrophs Methylocystis (15% RA) and Methylosinus (10% RA) and the denitrifiers Arenimonas (10% RA) and Pseudoxanthomonas (7% RA) were the most active genera. Obscuribacterales was the most active taxa in the community at 22% RA. Activity was confirmed by the Δ buoyant density changes with time for the taxa, indicating most of the community activity was associated with methane oxidation and subsequent consumption of methanotrophic metabolic intermediates by the denitrifiers. This is the first report of RNA stable isotope probing within a microaerobic methane driven denitrification system and the active community was markedly different from the full community identified via 16S-rDNA analysis.

Linking differences in microbial network structure with changes in coral larval settlement

Coral cover and recruitment have decreased on reefs worldwide due to climate change-related disturbances. Achieving reliable coral larval settlement under aquaculture conditions is critical for reef restoration programmes; however, this can be challenging due to the lack of reliable and universal larval settlement cues. To investigate the role of microorganisms in coral larval settlement, we undertook a settlement choice experiment with larvae of the coral Acropora tenuis and microbial biofilms grown for different periods on the reef and in aquaria. Biofilm community composition across conditioning types and time was profiled using 16S and 18S rRNA gene sequencing. Co-occurrence networks revealed that strong larval settlement correlated with diverse biofilm communities, with specific nodes in the network facilitating connections between modules comprised of low- vs high-settlement communities. Taxa associated with high-settlement communities were identified as Myxoccales sp., Granulosicoccus sp., Alcanivoraceae sp., unassigned JTB23 sp. (Gammaproteobacteria), and Pseudovibrio denitrificans. Meanwhile, taxa closely related to Reichenbachiella agariperforans, Pleurocapsa sp., Alcanivorax sp., Sneathiella limmimaris, as well as several diatom and brown algae were associated with low settlement. Our results characterise high-settlement biofilm communities and identify transitionary taxa that may develop settlement-inducing biofilms to improve coral larval settlement in aquaculture.

Effect of anode material and dispersal limitation on the performance and biofilm community in microbial electrolysis cells

In a microbial electrolysis cell (MEC), the oxidization of organic compounds is facilitated by an electrogenic biofilm on the anode surface. The biofilm community composition determines the function of the system. Both deterministic and stochastic factors affect the community, but the relative importance of different factors is poorly understood. Anode material is a deterministic factor as materials with different properties may select for different microorganisms. Ecological drift is a stochastic factor, which is amplified by dispersal limitation between communities. Here, we compared the effects of three anode materials (graphene, carbon cloth, and nickel) with the effect of dispersal limitation on the function and biofilm community assembly. Twelve MECs were operated for 56 days in four hydraulically connected loops and shotgun metagenomic sequencing was used to analyse the microbial community composition on the anode surfaces at the end of the experiment. The anode material was the most important factor affecting the performance of the MECs, explaining 54–80 % of the variance observed in peak current density, total electric charge generation, and start-up lag time, while dispersal limitation explained 10–16 % of the variance. Carbon cloth anodes had the highest current generation and shortest lag time. However, dispersal limitation was the most important factor affecting microbial community structure, explaining 61–98 % of the variance in community diversity, evenness, and the relative abundance of the most abundant taxa, while anode material explained 0–20 % of the variance. The biofilms contained nine Desulfobacterota metagenome-assembled genomes (MAGs), which made up 64–89 % of the communities and were likely responsible for electricity generation in the MECs. Different MAGs dominated in different MECs. Particularly two different genotypes related to Geobacter benzoatilyticus competed for dominance on the anodes and reached relative abundances up to 83 %. The winning genotype was the same in all MECs that were hydraulically connected irrespective of anode material used.

Can you see the algae for the slime? Temporal patterns of biofilm food quality and quantity in lowland rivers

AbstractAnimals must invest some portion of their metabolism to activities related to physiological maintenance and the remainder to processes related to the production of new biomass for growth and reproduction. Animal metabolism is fuelled by food, and the quality and quantity of food, along with the effort invested to obtain it, are fundamental to supporting populations. Biofilms are a primary basal food resource within riverine food webs, and it is thought that their nutritional value for animals decreases with age due to dynamic changes in community composition. We sought to test assumptions of spatiotemporal changes to biofilm nutritional value by assessing variations in biofilm mass and fatty acid composition in three rivers for 73 days. We also used a multi‐prong eDNA approach to characterize changes to biofilm fungal (ITS1–4), bacterial (16S), and algal (23S) community compositions. We anticipated biofilm food value to decrease with biofilm age due to shifts in composition from high‐quality green algae and diatoms to low‐quality cyanobacteria and filamentous algae. Our results partially support this contention; biofilm food value, assessed as a combination of fatty acid mass per unit area (in grams per square meter) and concentration of fatty acids (in milligrams per gram), was dynamic and peaked between 24 and 43 days following submersion. After 43 days, biofilm food value decreased. However, despite significant temporal changes in biofilm community composition and a decrease in overall lipid concentration, the proportions of different fatty acid classes among total lipids did not vary. Instead, the observed increase in the abundance of cyanobacteria and filamentous algae compared with diatoms and green algae, along with higher quantities of lipid‐poor extracellular polymeric substances (EPS), likely contributed to the reduction in overall lipid concentration relative to the biofilm dry mass. Here we present a novel approach to balance consumer energetic costs with food quality within aquatic food webs. Our results have important implications for river management and provide valuable information for the use of environmental water to support lotic ecosystems.

Biofilm community composition is changing in remote mountain lakes with a relative increase in potentially toxigenic algae

Mountain lakes provide clear drinking water to humankind but are strongly impacted by global change. Benthic biofilms are crucial for maintaining water quality in these oligotrophic lakes, yet little is known about the effects of global change on mountain biofilm communities. By combining analyses of metabarcoding data on 16S and 18S rRNA genes with climatic and environmental data, we investigated global change effects on the composition of biofilm prokaryotic and micro-eukaryotic assemblages in a five-year monitoring program of 26 Pyrenean lakes (2016–2020). Using time-decay relationships and within-lake dissimilarity modelling, we show that the composition of both prokaryotic and micro-eukaryotic biofilm communities significantly shifted and their biodiversity declined from 2016 to 2020. In particular, analyses of temporal trends with linear mixed models indicated an increase in the richness and relative abundance of cyanobacteria, including potentially toxigenic cyanobacteria, and a concomitant decrease in diatom richness and relative abundance. While these compositional shifts may be due to several drivers of global change acting simultaneously on mountain lake biota, water pH and hardness were, from our data, the main environmental variables associated with changes for both prokaryotic and micro-eukaryotic assemblages. Water pH and hardness increased in our lakes over the study period, and are known to increase in Pyrenean lakes due to the intensification of rock weathering as a result of climate change. Given predicted climate trends and if water pH and hardness do cause some changes in benthic biofilms, those changes might be further exacerbated in the future. Such biofilm compositional shifts may induce cascading effects in mountain food webs, threatening the resilience of the entire lake ecosystem. The rise in potentially toxigenic cyanobacteria also increases intoxication risks for humans, pets, wild animals, and livestock that use mountain lakes. Therefore, our study has implications for water quality, ecosystem health, public health, as well as local economies (pastoralism, tourism), and highlights the possible impacts of global change on mountain lakes.

Fluid flow structures gut microbiota biofilm communities by distributing public goods.

Bacterial gut commensals experience a biologically and physically complex mucosal environment. While many chemical factors mediate the composition and structure of these microbial communities, less is known about the role of mechanics. Here, we demonstrate that fluid flow impacts the spatial organization and composition of gut biofilm communities by shaping how different species interact metabolically. We first demonstrate that a model community composed of Bacteroides thetaiotaomicron (Bt) and Bacteroides fragilis (Bf), two representative human commensals, can form robust biofilms in flow. We identified dextran as a polysaccharide readily metabolized by Bt but not Bf, but whose fermentation generates a public good enabling Bf growth. By combining simulations with experiments, we demonstrate that in flow, Bt biofilms share dextran metabolic by-products, promoting Bf biofilm formation. By transporting this public good, flow structures the spatial organization of the community, positioning the Bf population downstream from Bt. We show that sufficiently strong flows abolish Bf biofilm formation by limiting the effective public good concentration at the surface. Physical factors such as flow may therefore contribute to the composition of intestinal microbial communities, potentially impacting host health.

Microbial Diversity and Activity of Biofilms from Geothermal Springs in Croatia

Hot spring biofilms are stable, highly complex microbial structures. They form at dynamic redox and light gradients and are composed of microorganisms adapted to the extreme temperatures and fluctuating geochemical conditions of geothermal environments. In Croatia, a large number of poorly investigated geothermal springs host biofilm communities. Here, we investigated the microbial community composition of biofilms collected over several seasons at 12 geothermal springs and wells. We found biofilm microbial communities to be temporally stable and highly dominated by Cyanobacteria in all but one high-temperature sampling site (Bizovac well). Of the physiochemical parameters recorded, temperature had the strongest influence on biofilm microbial community composition. Besides Cyanobacteria, the biofilms were mainly inhabited by Chloroflexota, Gammaproteobacteria, and Bacteroidota. In a series of incubations with Cyanobacteria-dominated biofilms from Tuhelj spring and Chloroflexota- and Pseudomonadota-dominated biofilms from Bizovac well, we stimulated either chemoorganotrophic or chemolithotrophic community members, to determine the fraction of microorganisms dependent on organic carbon (in situ predominantly produced via photosynthesis) versus energy derived from geochemical redox gradients (here simulated by addition of thiosulfate). We found surprisingly similar levels of activity in response to all substrates in these two distinct biofilm communities, and observed microbial community composition and hot spring geochemistry to be poor predictors of microbial activity in the study systems.

Early succession of biofilm bacterial communities in newly built drinking water pipelines via multi-area analysis

Biofilms inhabiting pipeline walls are critical to drinking water quality and safety. With massive pipeline replacement underway, however, biofilm formation process in newly built pipes and its effects on water quality are unclear. Moreover, differences and connections between biofilms in newly built and old pipes are unknown. In this study, early succession (≤ 120days) of biofilm bacterial communities (abundance and diversity) in upper, middle and bottom areas of a newly built cement-lined ductile iron pipeline were evaluated using improved Propella™ biofilm reactor and multi-area analysis. A comparison with old pipelines (grey cast iron, 10years) was performed. In the newly built pipeline, the abundance of biofilm bacteria did not change significantly between 40 and 80days, but increased significantly between 80 and 120days. The biofilm bacterial abundance (per unit area) in the bottom area was always higher than that in the upper and middle areas. Based on alpha diversity index and PCoA results, biofilm bacterial community richness, diversity and composition did not change significantly during the 120-day operation. Besides, biofilm shedding from the walls of newly built pipeline significantly increased bacterial abundance in the outlet water. Opportunistic pathogen-containing genera, such as Burkholderia, Acinetobacter and Legionella, were identified in both water and biofilm samples from newly built pipelines. The comparison between new and old pipelines suggested a higher bacterial abundance per unit area at the middle and bottom areas in old pipelines. Moreover, the bacterial community composition of biofilms in old pipelines was similar to that of newly built pipelines. These results contribute to accurate prediction and management of biofilm microbial communities in drinking water pipelines, ensuring the biosafety of drinking water. KEY POINTS: • Biofilm bacterial communities in different areas of pipe wall were revealed. • The abundance of biofilm bacteria increased significantly between 80 and 120days. • Biofilm bacterial community compositions of newly built and old pipes were similar.

Ocean acidification alters the benthic biofilm communities in intertidal soft sediments

Microphytobenthos (MPB) and bacterial biofilms play crucial roles in primary and secondary production, nutrient cycling and invertebrate settlement in coastal ecosystems, yet little is known of the effects of ocean acidification (OA) on these communities in intertidal soft sediments. To fill in this gap, a 28-day CO2 enhancement experiment was conducted for the benthic biofilms in soft intertidal sediments (muds and sands) from Qingdao, China. This experiment included three CO2 treatments: 400 ppm CO2 (control), 700 ppm CO2 and 1000 ppm CO2 (IPCC predicted value in 2100), which were established in a three-level CO2 incubator that can adjust the CO2 concentration in the overlying air. The effects of OA on benthic biofilms were assessed in the following three aspects: MPB biomass, biofilm community structure and microbial biogeochemical cycling (e.g., C-cycle, N-cycle and S-cycle). This study found that the 700 ppm CO2 treatment did not significantly affect the benthic biofilms in intertidal soft sediments, but the 1000 ppm CO2 treatment significantly altered the biofilm community composition and potentially their role in microbial biogeochemical cyc\ling in sediments (especially in sandy sediments). For the bacterial community in biofilms, the 1000 ppm CO2 enhancement increased the relative abundance of Alteromonadales and Bacillales but decreased the relative abundance of Rhodobacterales and Flavobacteriales. For microbial biogeochemical cycling, the 1000 ppm CO2 treatment enhanced the potential of chemoheterotrophic activity, nitrate reduction and sulfur respiration in sediments, likely resulting in a more stressful environment (hypoxic and enriched H2S) for most benthic organisms. Even though incubations in this study were only 28 days long and thus couldn’t fully accommodate the range of longer-term adaptions, it still suggests that benthic biofilms in intertidal sandy sediments are likely to change significantly near the end of the century if anthropogenic CO2 emissions unmitigated, with profound implications on local ecosystems and biogeochemical cycling.

Ecosystem responses of shallow thermokarst lakes to climate-driven hydrological change: Insights from long-term monitoring of periphytic diatom community composition at Old Crow Flats (Yukon, Canada).

Shallow waterbodies are abundant in Arctic and subarctic landscapes where they provide productive wildlife habitat and hold cultural and socioeconomic importance for Indigenous communities. Their vulnerability to climate-driven hydrological and limnological changes enhances a need for long-term monitoring data capable of tracking aquatic ecosystem responses. Here, we evaluate biological and inferred physicochemical responses associated with a rise in rainfall-generated runoff and increasingly positive lake water balances in Old Crow Flats (OCF), a 5600 km2 thermokarst landscape in northern Yukon. This is achieved by analyzing periphytic diatom community composition in biofilms accrued on artificial-substrate samplers at 14 lakes collected mostly annually during 2008-2019 CE. Results reveal that diatom communities at 10 of the 14 lakes converged toward a composition typical of lakes with rainfall-dominated input waters. These include six of nine lakes that were not initially dominated by rainfall input. The shifts in diatom community composition infer rise of lake-water pH and ionic content, and they reveal that northern shallow lake ecosystems are responsive to climate-driven increases in rainfall. Based on data generated during the 12 -year-long monitoring period, we conclude that lakes located centrally within OCF are most vulnerable to rapid climate-driven hydroecological change due to flat terrain, larger lake surface area, and sparse terrestrial vegetation, which provide less resistance to lake expansion, shoreline erosion, and sudden drainage. This information assists the local Indigenous community and natural resource stewardship agencies to anticipate changes to traditional food sources and inform adaptation options.

Effects of combining flow intermittency and exposure to emerging contaminants on the composition and metabolic response of streambed biofilm bacterial communities

Freshwater ecosystems are characterised by the co-occurrence of stressors that simultaneously affect the biota. Among these, flow intermittency and chemical pollution severely impair the diversity and functioning of streambed bacterial communities. Using an artificial streams mesocosm facility, this study examined how desiccation and pollution caused by emerging contaminants affect the composition of stream biofilm bacterial communities, their metabolic profiles, and interactions with their environment. Through an integrative analysis of the composition of biofilm communities, characterization of their metabolome and composition of the dissolved organic matter, we found strong genotype-to-phenotype interconnections. The strongest correlation was found between the composition and metabolism of the bacterial community, both of which were influenced by incubation time and desiccation. Unexpectedly, no effect of the emerging contaminants was observed, which was due to the low concentration of the emerging contaminants and the dominant impact of desiccation. However, biofilm bacterial communities modified the chemical composition of their environment under the effect of pollution. Considering the tentatively identified classes of metabolites, we hypothesised that the biofilm response to desiccation was mainly intracellular while the response to chemical pollution was extracellular. The present study demonstrates that metabolite and dissolved organic matter profiling may be effectively integrated with compositional analysis of stream biofilm communities to yield a more complete picture of changes in response to stressors.

The degradation of glyphosate is enhanced in a microbial fuel cell: Electrochemical performance, degradation efficiency, and analysis of the anodic microbial community

Glyphosate, one of the most used herbicides worldwide, is known as an aquatic contaminant of concern, and has been identified as presenting adverse impacts in agroecosystems, due to a somewhat limited natural chemical and biological degradation in the environment. In this study, we investigated the degradation of glyphosate in microbial electrochemical systems (MESs), and compared the performance and the microbial composition of enriched anodic biofilms with those shown by native microbial communities. The reduction of glyphosate content observed in MESs (approx. 70 %) was much higher than in non-electroactive microbial cultures (approx. 49 %). The analysis of the microbial communities by 16S amplicon sequencing revealed a significant difference between the microbial community composition of MESs anodic biofilms and non-electroactive enriched communities. The anodic biofilms were dominated by Rhodococcus (51.26 %), Pseudomonas (10.77 %), and Geobacter (8.67 %) while in non-MESs cultures, methanogens including Methanobrevibacter (51.18 %), and Methanobacterium (10.32 %), were the dominant genera. The present study suggested that MESs could be considered as a promising system for complete degradation of glyphosate from waters polluted by this herbicide.

Synthetic and biological surfactant effects on freshwater biofilm community composition and metabolic activity

Surfactants are used to control microbial biofilms in industrial and medical settings. Their known toxicity on aquatic biota, and their longevity in the environment, has encouraged research on biodegradable alternatives such as rhamnolipids. While previous research has investigated the effects of biological surfactants on single species biofilms, there remains a lack of information regarding the effects of synthetic and biological surfactants in freshwater ecosystems. We conducted a mesocosm experiment to test how the surfactant sodium dodecyl sulfate (SDS) and the biological surfactant rhamnolipid altered community composition and metabolic activity of freshwater biofilms. Biofilms were cultured in the flumes using lake water from Lake Lunz in Austria, under high (300 ppm) and low (150 ppm) concentrations of either surfactant over a four-week period. Our results show that both surfactants significantly affected microbial diversity. Up to 36% of microbial operational taxonomic units were lost after surfactant exposure. Rhamnolipid exposure also increased the production of the extracellular enzymes, leucine aminopeptidase, and glucosidase, while SDS exposure reduced leucine aminopeptidase and glucosidase. This study demonstrates that exposure of freshwater biofilms to chemical and biological surfactants caused a reduction of microbial diversity and changes in biofilm metabolism, exemplified by shifts in extracellular enzyme activities.Key points• Microbial biofilm diversity decreased significantly after surfactant exposure.• Exposure to either surfactant altered extracellular enzyme activity.• Overall metabolic activity was not altered, suggesting functional redundancy.

Effect of Calcium Ion Supplementation on Oral Microbial Composition and Biofilm Formation In Vitro.

The oral cavity contains a variety of ecological niches with very different environmental conditions that shape biofilm structure and composition. The space between the periodontal tissue and the tooth surface supports a unique anaerobic microenvironment that is bathed in the nutrient-rich gingival crevicular fluid (GCF). During the development of periodontitis, this environment changes and clinical findings reported a sustained level of calcium ion concentration in the GCF collected from the periodontal pockets of periodontitis patients. Here, we report the effect of calcium ion supplementation on human oral microbial biofilm formation and community composition employing an established SHI medium-based in vitro model system. Saliva-derived human microbial biofilms cultured in calcium-supplemented SHI medium (SHICa) exhibited a significant dose-dependent increase in biomass and metabolic activity. The effect of SHICa medium on the microbial community composition was evaluated by 16S rRNA gene sequencing using saliva-derived microbial biofilms from healthy donors and periodontitis subjects. In this study, intracellular microbial genomic DNA (iDNA) and extracellular DNA (eDNA) were analyzed separately at the genus level. Calcium supplementation of SHI medium had a differential impact on iDNA and eDNA in the biofilms derived from healthy individuals compared to those from periodontitis subjects. In particular, the genus-level composition of the eDNA portion was distinct between the different biofilms. This study demonstrated the effect of calcium in a unique microenvironment on oral microbial complex supporting the dynamic transformation and biofilm formation.

Impact of synthetic silver nanoparticles on the biofilm microbial communities and wastewater treatment efficiency in experimental hybrid filter system treating municipal wastewater

Silver nanoparticles (AgNPs) threaten human and ecosystem health, and are among the most widely used engineered nanomaterials that reach wastewater during production, usage, and disposal phases. This study evaluated the effect of a 100-fold increase in collargol (protein-coated AgNP) and Ag+ ions concentrations in municipal wastewater on the microbial community composition of the filter material biofilms (FMB) and the purification efficiency of the hybrid treatment system consisting of vertical (VF) and horizontal (HF) subsurface flow filters. We found that increased amounts of collargol and AgNO3 in wastewater had a modest effect on the prokaryotic community composition in FMB and did not significantly affect the performance of the studied system. Regardless of how Ag was introduced, 99.9% of it was removed by the system. AgNPs and AgNO3 concentrations did not significantly affect the purification efficiency of the system. AgNO3 induced a higher increase in the genetic potential of certain Ag resistance mechanisms in VFs than collargol; however, the increase in Ag resistance potential was similar for both substances in HF. Hence, the microbial community composition in biofilms of vertical and horizontal flow filters is largely resistant, resilient, or functionally redundant in response to AgNPs addition in the form of collargol.

Characterizing and comparing microbial community and biofilm structure in three nitrifying moving bed biofilm reactors

This study investigated biofilm establishment, biofilm structure, and microbial community composition of biofilms in three laboratory-scale moving bed biofilm reactors. These reactors were filled with three types of plastic carriers with varied depths of living space for microbial growth. The reactors were operated under the same influent and operational conditions. Along with the operation, the results showed that carriers with grids of 50 μm in height delayed the biofilm development and formed the thinnest biofilm and a carpet-like structure with the lowest α-diversity. In comparison, another two carriers with grids of 200 and 400 μm in height formed thick biofilms and large colonies with more voids and channels. Quantified properties of biofilm thickness, biomass, heterogeneity, portion of the biofilm exposed to the nutrient, and maximum diffusion distance were examined, and the results demonstrated that they almost (except for heterogeneity) strongly correlated to the α-diversity of microbial community. These illustrate that depth of living space, as an important parameter for carrier, could drive the formation of biofilm structure and community composition. It improves understanding of influencing factors on biofilm establishment, structure and its microbial community, and would be helpful for the design of biofilm processes.

The effect of time and surface type on the composition of biofilm communities on concrete exposed to seawater

Microbially induced deterioration is a threat to concrete infrastructures in marine environments. Complex microbial biofilms form on concrete surfaces exposed to sea water and cause material deterioration. However, the mechanisms determining the composition and the development of the biofilm communities are poorly understood. We designed a mesocosm experiment to determine the influence of concrete surface structure (smooth/rough) and steel fiber reinforcement (presence/absence) on biofilm development over a period of 455 days. This enabled a novel methodology to systematically assess biofilm formation of bacteria on concrete exposed to marine water. The biofilm communities were distinctly different from the planktonic communities in the systems. The alpha diversity increased with time and longer time intervals correlated with higher turnover of taxa. Several taxa within Caulobacterales and Rhodobacterales were identified as early biofilm formers, which decreased in relative abundance and were replaced by taxa within Planctomycetales as the biofilm developed. Throughout the experimental period, concrete surface type influenced the microbial community composition. Some taxa such as Magnetospiraceae, Portibacter, Rubripirellula, and Rhodopirellula, possibly involved in the oxidation and reduction of iron, were for instance more abundant in biofilms on steel-fiber containing concrete. Null models suggested that mainly deterministic factors were shaping the microbial communities with limited importance of stochasticity at shorter time intervals.

Detection of biofilm and planktonic microbial communities in litter/soil mixtures

Microbial community of the soil can be subdivided into a planktonic community of motile organisms and a community of sessile organisms, including those constituting biofilms on physical surfaces of different texture. Biofilm communities on surfaces of organic particles represent a crucial factor involved in decomposition of plant litter and soil particulate organic matter in general. In spite of their great ecological importance, the composition of soil biofilm communities is only scarcely studied due to methodological problems connected with distinguishing biofilm inhabitants from other soil organisms.In this study, we were able to distinguish microbial communities colonizing four compartments of the experimental system: particles of the litter/soil mixture, biofilms on adjacent smooth or fibrous glass surfaces, and plankton. Existence of different preferences of microbial operational taxonomic units (OTUs) to occupy the experimental compartments has been suggested on the basis of their relative abundance in the community. Three main OTU groups have been defined according to these preferences. They are tentatively termed generalists, OTUs corresponding to organisms preferring the litter particles and OTUs corresponding to selective organisms discriminating between different surface types. At the same time, temperature has been identified as a factor influencing the switching between preferences of organisms for experimental compartments. Our work contributes to the description of the spatial microscale distribution of microorganisms in soil containing decomposing organic matter.