Biofertilizer Inoculation Research Articles

Impact of Liquid Bio-Fertilizers on Okra (Abelmoschus esculentus L. Moench.) Plant Growth Indices using Azotobacteria and Azospirillum Consortia

This study was to make bio-fertilizers with Azotobacteria and Azospirilum consortiums and to test the influence of bio-fertilizers on Okra plant growth. This investigation was conducted using the soil from the Biological Garden of the University of Cross River State, Calabar. Pure culture and mass production was performed in batch fermentation at optimum condition by means of specific medium for Azospirilum and Azotobacter. Organisms were isolated from the soil samples and confirmed using biochemical tests. The bio-fertilizer effect on the growth parameters of Okra plants was carried out on Day 21. The post germination, phenotypic and observations showed significant differences in performance between plant length, mass, root numbers, root length, total plant length, breadth of leaves, shoot length, root mass and biological yield. The biochemical indices exibited a significant rise (P < 0.05) in chlorophyll content (mg/g.fr. wt) as compared to Control (0.010±0.000), Azospirilum (0.016±0.000), Azotobacter (0.016±0.000) and consortium (0.018±0.000). Significant rise (P<0.05) in carbohydrate content (%) control (51.976±0.768), Azospirilum (69.690±3), Azotobacter (69).656+2), and consortium 70).190± (5). Protein content control (0.028±0.001), Azospirilum (0.040±0.000), Azotobacter (0.060±0.001) and consortium (0.061±0.000) all significantly increased [P< 0.05]. Growth is significantly affected by single injection and control when compared to plants being treated with Azotobacter, Azospirilum or both (P < 0.05). According to the findings, plant mineral fixation through nitrogen fixation and phosphorous solubilization seems to be improved by bio-fertilizer inoculation as well as their mineral nutrition’s improvement.

Interactive Effect of Bio-Organic and Inorganic Fertilizers on Crop Growth, Quality, Productivity and Profitability of Groundnut (Arachis Hypogeae L.) in an Alfisols of Western-Ghat of Maharashtra India

ABSTRACT Precisely because of low nutrient availability and toxicities of nutrients unfertilized alfisols are unable to satisfy the nutrients requirement to meet plants demand. An investigation was carried out to assess the impact of biofertilizer inoculation (no biofertilizers, arbuscular mycorrhizal fungi (AMF) @ 10 kg ha−1, phosphate solubilizing bacteria (PSB) @ 10 kg ha−1, AMF+PSB @ 5 kg each ha−1 and AMF+PSB @ 10 kg each ha−1) and phosphorus levels (15, 30, 45, and 60 kg ha−1) on growth, quality, productivity and profitability of groundnut in alfisols of Western Ghats of Maharashtra, India, in a factorial randomized block design during 2017–18 and 2018–19 growing seasons. It was discovered that groundnuts grew better with 60 kg phosphorus and 10 kg each AMF+PSB ha−1 and produced significantly higher pod yield (35.86 q ha−1), kernel yield (2.52 t ha−1) and harvest index (41.47%). However, inoculation and their interaction did not reach the level of significance, despite the fact that they responded favorably to the high phosphorus (60 kg ha−1) rate. Similarly, plots infused with 60 kg phosphorus ha−1 and AMF+PSB 10 kg each ha−1 significantly exhibited highest oil content (50.62%), protein content (31.93%), total nitrogen (269.71 kg ha−1), phosphorus (8.60 kg ha−1) uptake, nutrient efficiency ratio (40.79 kg kg−1), nutrient harvest index (44.11%), gross return (INR 2,44,388 ha−1), net return (INR 62,209 ha−1), and benefit: cost ratio (B:C ratio) of 1.96. In view of their intense need for nutrients, groundnuts thrived well when given an appropriate combination of inorganic and biological fertilizers.

Multi-omics analysis reveals the effects of three application modes of plant growth promoting microbes biofertilizer on potato (Solanum tuberosum L.) growth under alkaline loess conditions

Potato is an important crop due to its high contents of starch, protein, and various vitamins and minerals. Biofertilizers are composed of plant growth promoting microbes (PGPMs) which are essential for improving the growth and resistance of potato. However, little information has focused on the modes of inoculation of biofertilizers on plant growth and microecology. This study aims to reveal the response mechanism of the potato to three modes of inoculation of biofertilizers all containing PGPM Bacillus amyloliquefaciens EZ99, i.e. scattered mode of 5 kg/ha biofertilizer (M5), soaking seed tubers with dissolved 5 kg/ha biofertilizer (MZG), and scattered mode of 3 kg/ha biofertilizer + 2 kg/ha sucrose (MY34) in alkaline loess field through multi-omics analysis of transcriptome, metabolome and microbiome. The physiological result revealed that two application modes of equal amount of biofertilizer M5 and MZG significantly improved the growth and yield of potatoes. Furthermore, the transcriptome of potato exhibited sets of differentially expressed genes enriched in photosynthesis, sugar metabolism, and phenylpropanoid biosynthesis among the three modes, with the M5 mode exhibiting overall up-regulation of 828 genes. Based on the untargeted metabolomic analysis of potato tuber, M5 mode significantly accumulated sucrose, while MZG and MY34 mode significantly accumulated the stress metabolites euchrenone b6 and mannobiose, respectively. Besides, the microbial structure of potato rhizosphere showed that the diversity of bacteria and fungi was similar in all soils, but their abundances varied significantly. Specifically, beneficial Penicillium was enriched in M5 and MZG soils, whereas MY34 soil accumulated potential pathogens Plectosphaerella and saccharophilic Mortierella. Collectively, these e findings highlight that MZG is the most effective mode to promote potato growth and stimulate rhizosphere effect. The present study not only encourages sustainable agriculture through agroecological practices, but also provides broad prospects for the application of PGPM biofertilizer in staple foods.

Quantifying response of azospirillum and phosphate solubilizing bacteria liquid inoculants seed treatment on productivity and profitability of kharif grain sorghum (Sorghum bicolor)

A field study was carried out during 2017–19 at Sorghum Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra to assess the effect of liquid bio-fertilizers inoculation over existing powder form inoculants on kharif grain sorghum [Sorghum bicolor (L.) Moench]. The experiment comprised of 6 treatments, viz. no bio-fertilizer control i.e. with RDF alone (T1 ); RDF + Azo. (Azospirillum) + PSB @50 g each/kg seed (T2 ); RDF + Azo. + PSB @2 ml each/kg seed (T3 ); RDF + Azo. @2 ml and PSB @4 ml/kg seed (T4 ); RDF + Azo. @4 ml + PSB @2 ml/kg seed (T5 ); and RDF + Azo. + PSB @4 ml each/kg seed (T6) conducted in randomized block design (RBD) with 4 replications. Pooled data revealed that seed inoculation with Azo. + PSB @2 ml each/kg seed (T3 ) has improved the sorghum biological, grain yield and net returns 21.13, 19.83 and 17.92% over their powder form of inoculants i.e. T2 (14.71 t/ha, 3.56 t/ha and 73,116). These enhancements in biomass, grain yields and net income was owing to liquid bio-fertilizer seed treatment. Liquid-based inoculants were found to boost growth and yield parameters, which was ascribed to the concomitant increase in plant height and number of grains/panicle, respectively. Increase in quantity of liquid bio-fertilizers beyond 2 ml/kg seed proved not-rewarding from both productivity and profitability point of view. Sorghum seed inoculation with liquid bio-fertilizers @2 ml each/kg seed proved to be a potential substitute for existing powder form without any additional cost.

Native Liquid Biofertilizer Consortia of Staphylococcus equorum, Bacillus velezensis and Staphylococcus epidermidis Enhanced the Growth of Nutmeg Seedlings

Aims: The aim was to evaluate the three most distinct native liquid biofertilizer consortia on the growth of nutmeg seedlings under pot culture studies. Study Design: Completely Randomized Block Design. Place and Duration of Study: Department of Agricultural Microbiology, College of Agriculture, Vellanikkara, Thrissur, Kerala and Block 10, Regional Agricultural Research Station, Ambalavayal, Wayanad, Kerala. The pot culture experiment was conducted from February 2023 to August 2023. Methodology: The experiment was carried out in a completely randomized block design with three replications along with control. Each treatment was applied with a combination of NPK fertilizers (50% and 75% of recommended dose of fertilizer, based on soil test data). One treatment was composed of only organic fertilizers. Results: The biofertilizer inoculation had a positive impact on the population of soil microbes, which indicates good soil health. Application of Staphylococcus equorum + Bacillus velezensis + Staphylococcus epidermidis (consortium – 2) along with 50% of the recommended dose of NPK fertilizers, recorded the highest plant growth parameters viz., plant height, leaf length, leaf breadth, stem diameter, leaf area, shoot dry weight, and maximum number of secondary roots. In most of the treatments, nutmeg shoot growth was higher when compared to root growth. Conclusion: The present study indicated that inoculation of native liquid biofertilizer consortia enhanced the growth of nutmeg seedlings under pot culture studies in Wayanad. Hence liquid biofertilizer consortia can be utilized for earlier establishment of nutmeg seedlings and thereby reduce the dependency on the quantity of chemical fertilizers.

Diversity of the Maize Root Endosphere and Rhizosphere Microbiomes Modulated by the Inoculation with Pseudomonas fluorescens UM270 in a Milpa System.

Milpa is an agroecological production system based on the polyculture of plant species, with corn featuring as a central component. Traditionally, the milpa system does not require the application of chemicals, and so pest attacks and poor growth in poor soils can have adverse effects on its production. Therefore, the application of bioinoculants could be a strategy for improving crop growth and health; however, the effect of external inoculant agents on the endemic microbiota associated with corn has not been extensively studied. Here, the objective of this work was to fertilize a maize crop under a milpa agrosystem with the PGPR Pseudomonas fluorescens UM270, evaluating its impact on the diversity of the rhizosphere (rhizobiome) and root endophytic (root endobiome) microbiomes of maize plants. The endobiome of maize roots was evaluated by 16S rRNA and internal transcribed spacer region (ITS) sequencing, and the rhizobiome was assessed by metagenomic sequencing upon inoculation with the strain UM270. The results showed that UM270 inoculation of the rhizosphere of P. fluorescens UM270 did not increase alpha diversity in either the monoculture or milpa, but it did alter the endophytic microbiome of maize plant roots by stimulating the presence of bacterial operational taxonomic units (OTUs) of the genera Burkholderia and Pseudomonas (in a monoculture), whereas, in the milpa system, the PGPR stimulated greater endophytic diversity and the presence of genera such as Burkholderia, Variovorax, and N-fixing rhizobia genera, including Rhizobium, Mesorhizobium, and Bradyrhizobium. No clear association was found between fungal diversity and the presence of strain UM270, but beneficial fungi, such as Rizophagus irregularis and Exophiala pisciphila, were detected in the Milpa system. In addition, network analysis revealed unique interactions with species such as Stenotrophomonas sp., Burkholderia xenovorans, and Sphingobium yanoikuyae, which could potentially play beneficial roles in the plant. Finally, the UM270 strain does not seem to have a strong impact on the microbial diversity of the rhizosphere, but it does have a strong impact on some functions, such as trehalose synthesis, ammonium assimilation, and polyamine metabolism. The inoculation of UM270 biofertilizer in maize plants modifies the rhizo- and endophytic microbiomes with a high potential for stimulating plant growth and health in agroecological crop models.

Use of systemic biofertilizers in sugarcane results in highly reproducible increments in yield and quality of harvests

The utilization of a novel (systemic) biofertilizer containing Pseudomonas fluorescens, Azospirillum brasilense, and Bacillus subtilis and possessing the technology to facilitate the entry of bacteria through the stomata, was evaluated at three localities in Mexico (Potrero Nuevo, Veracruz; Ameca, Jalisco; and Champotón, Campeche) in two sugarcane varieties (NCO-310 and Mex 57–473) at different time scales. Inoculation of the systemic biofertilizer was imposed over the local agricultural management of the sugarcane; chemical fertilization of the experimental parcels at Potrero Nuevo was done using 70-20-20 and 120-80-80 at Ameca and Champotón. Three doses of the biofertilizer per hectare were applied during the annual productive cycle of sugarcane at each site; one year at Potrero Nuevo and Champotón; and six years at Ameca. The annual sugarcane yield was evaluated at each site. Additionally, sugar quality (°Brix or sucrose content) was evaluated at the three localities, while different variables of stalk performance were also measured at Ameca and Champotón. Our data provide evidence that this systemic biofertilizer consistently and reliably increased the sugarcane yield at all localities during the time of evaluation, ranging from 73.7 tons ha−1 at Potrero Nuevo (2.5 times increase; P < 0.05) and 77.7 tons ha−1 at Ameca (1.9 times increase; P < 0.05) to 23.8 tons ha−1 at Champotón (1.4 times increase; P < 0.05). This increase in sugarcane biomass was related to increased tillering rather than increased stalk height or diameter. This novel biological product improved the sugarcane quality in terms of °Brix (P < 0.05, 2.6° difference) and sucrose content (P < 0.5, 0.7% difference).

Cyanobacterial biofertilizer inoculation has a distinctive effect on the key genes of carbon and nitrogen cycling in paddy rice

Cyanobacterial biofertilizer inoculation has a distinctive effect on the key genes of carbon and nitrogen cycling in paddy rice

Mitigation of arsenic accumulation in crop plants using biofertilizer.

Elevated levels of arsenic in crop plants have been found in various regions worldwide, especially where agricultural soils have been affected by arsenic-enriched aquifers and human activities including mining, smelting, and pesticide application. Given the highly toxic nature of arsenic, remediation should be carried out immediately to reduce this potentially toxic element transport from soil to crop plants. This study focused on the utilization of biofertilizer which is a combination of arsenic-accumulating microorganisms and adsorbent (carrier) in order to achieve high efficiency of arsenic immobilization and ability to apply in the field. Thirty-two bacterial strains were isolated from 9 soil samples collected from the Dongjin and Duckum mining areas in Korea using a nutrient medium amended with 2 mM sodium arsenite. Among isolates, strain DE12 identified as Bacillus megaterium exhibited the greatest arsenic accumulation capacity (0.236 mg/g dry biomass) and ability to resist up to 18 mM arsenite. Among the three agricultural waste adsorbents studied, rice straw was proved to have a higher adsorption capacity (0.104 mg/g) than rice husk and corn husk. Therefore, rice straw was chosen to be the carrier to form biofertilizer together with strain DE12. Inoculation of biofertilizer in soil showed a reduction of arsenic content in the edible part of lettuce, water spinach, and sweet basil by 17.5%, 34.1%, and 34,1%, respectively compared to the control group. The use of biofertilizer may open up the potential application in the field for other food plants.

Effectiveness of Rhizobium tropici sp. Strain UD5 Peat Biofertilizer Inoculant on Growth, Yield, and Nitrogen Concentration of Common Bean

Common bean (Phaseolus vulgaris L.) ranks among the most produced and consumed legume crops and contains essential macro- and micronutrients. Grain yield of the food crop is markedly decreased by poor management, especially a lack of additional essential nutrient elements through the application of fertilizers. In addition to the application of fertilizers, scholarly research and crop farmers have shown that the use of biofertilizer inoculants improves the yield of legume crops. The objective of this research study was to assess the effectiveness of peat-based Rhizobium tropici sp. UD5 on the growth, yield, and nitrogen concentration of common bean. The peat inoculant contained 6.5 × 109 viable cells/g. The experiment was conducted in two climatic zones, as described by the Koppen–Gieger climatic classification system. Treatments involved the peat-based inoculant Rhizobium tropici (T0 = 0 g without inoculation, T1 = 250 g of peat inoculant of strain UD5 for 50 kg seeds, T2 = 500 g of inoculant of strain UD5, and T3 = 200 g of comparative peat inoculant). The results indicated that common-bean-inoculated formulation of R. tropici sp. strain UD5 increased the following parameters compared to the controls: plant height (T1 = 18.22%, T2 = 20.41%, and T3 = 19.93% for bioclimatic zone 1; T1 = 16.78%, T2 = 20.71%, and T3 = 19.93% for bioclimatic zone 2), root length (T1 = 13.26%, T2 = 21.28%, and T3 = 19.38% for zone 1; T1 = 15.06%, T2 = 23.70%, and T3 = 19.20% for zone 2), number of nodules (T1 = 1162.57%, T2 = 1166.36%, and T3 = 1180.30% for zone 1; T1 = 1575%, T2 = 1616.5%, and T3 = 1608.25% for zone 2), size of nodules (T1 = 224.07%, T2 = 224.07%, and T3 = 208.33% for zone 1; T1 = 166.4%, T2 = 180%, and T3 = 140% for zone 2), and yield (T1 = 40.49%, T2 = 47.10%, and T3 = 45.45% for zone 1; T1 = 62.16%, T2 = 54.05%, and T3 = 58.55% for zone 2). R. tropici sp. UD5 peat inoculant formulation also increased the nitrogen concentration in leaves compared to the control (T1 = 3.75%, T2 = 1.12%, and T3 = 8.72%) in both bioclimatic zones. The findings of this study provide significant information on the positive effect of R. tropic UD5 strain peat inoculant application in the improvement of plant growth, development, and yield through the formation of nodules.

Soil Physical and Chemical Properties as Affected by Bio-, Organic and Inorganic NPSB Fertilizers and Lime in Assosa Zone, Western Ethiopia

Background: Soil acidity, low soil nutrient status and low nitrogen fixing inoculants as a result of poor soil fertility management practices are the major constraints in soybean production in Assosa Zone, particularly in Assosa and Bambassi districts. Hence, this experiment was conducted to evaluate the effect of bio-, organic and inorganic NPSB fertilizers and lime on soil physico- chemical properties. Methods: During the period 2019-2020 factorial combinations of two bio-fertilizer inoculants [without bio-fertilizer (B1) and SB12 plus MAR1495 bio-fertilizer at their recommended rates of 500 g ha-1 (B2); two organic fertilizers without fresh cattle manure (M1) and fresh cattle manure at 10 t ha-1 (M2); two lime rates without lime (L1) and lime at 5 t ha-1 (L2); two inorganic NPSB fertilizers NPSB at 9.5-23-3.5-0.05 (F1) and NPSB at 19-46-7-0.1 (F2) at their recommended rates for soybean] were laid out in a randomized complete block design with three replications. Bulk density, pH, total nitrogen, available phosphorus, organic carbon and cation exchange capacity were collected and analyzed using SAS software version 9.1.3 and significant mean differences were separated using Duncan’s Multiple Range Test (DMRT). Result: Bulk density, pH, total nitrogen, available phosphorus, organic carbon and cation exchange capacity were highly affected (P less than 0.01) by the interactions of bio-, organic and inorganic fertilizers and lime. Finally, the interaction of SB12+MAR1495, 10t/ha fresh cattie manure, 5t/ha lime and NPSB at 19-46-7-0.1 improved the bulk density, pH, total nitrogen, available phosphorus, organic carbon and cation exchange capacity at both districts.

Prospective bioencapsulation to improve the quality of phosphate-solubilizing biofertilizers for crop production: A review

Farmers commonly use chemical phosphorus fertilizer to increase crop yields, but long-term use may harm the environment. Biofertilizers specifically phosphate solubilizing microbes (PSMs), are used for restoring soil fertility in crop production. PSMs contribute to plant phosphorus supply by increasing phosphorus availability in the soil through organic acid and phosphatase enzyme secretion. However, the conventional biofertilizer formulation is often limited by a short shelf-life followed by a decreased microbial effectiveness. In recent years, bioencapsulation formulation have received more attention because they offer products that have a longer shelf-life, protect microbes in harsh environments, and maintain the effectiveness of biofertilizers. This literature review focuses on the latest bioencapsulation technology, the impact of bioencapsulation on phosphate-solubilizing biofertilizers, and the potential for developing bioencapsulation-based biofertilizers for sustainable agriculture. Precise formulation is crucial in bioencapsulation, influencing the quality and performance of biofertilizer inoculants. Bioencapsulated biofertilizers offer advantages such as improved nutrient management, enhanced plant stress tolerance, disease suppression, and innovative agricultural waste management. Despite longer shelf life and adaptability to various crops, further research is needed to address scalability, cost-effectiveness, and standardized encapsulation techniques. In conclusion, bioencapsulation innovations have the potential to promote sustainable and productive crop production.

Applications of Biofertilizers and Phosphorus Influence Nutrient Uptake and Nutrient Use Efficiency in Chickpea (Cicer Arietinum L.)

ABSTRACT The objective of the study was to investigate the effects of sole applications of biofertilizers and phosphorus as well as their integrated use in lentil. The field experiment was conducted to study the effect of phosphorus (0, 15, 20 and 25 kg P2O5 ha−1) and biofertilizer inoculation treatments [uninoculated control, Mesorhizobium, Mesorhizobium + RB-1 (Pseudomonas argentinensis) and Mesorhizobium + RB-2 (Bacillus aryabhattai)] on nutrient uptake, nutrient use efficiency and residual nutrient status of soil after the harvest of chickpea. Mesorhizobium + RB-1 recorded the highest uptake of nitrogen (73.7, 59.0 and 132.7 kg ha−1 in grain, straw and total, respectively), phosphorus (8.29, 11.34 and 19.63 kg ha−1 in grain, straw and total) and potassium (23.48, 43.36 and 66.84 kg ha−1 in grain, straw and total) as well as partial factor productivity (113.1 kg grain kg−1 P2O5). Application of 25 kg P2O5 ha−1 recorded the highest phosphorus concentration (0.39% in grain and 0.26% in straw), phosphorus uptake (8.35, 12.32 and 20.67 kg ha−1 in grain, straw and total, respectively) and phosphorus status in soil after crop harvest (18.7 and 16.7 kg P ha−1 in 0–15 and 15–30 cm soil layers, respectively). Partial factor productivity was recorded the highest with the application of 15 kg P2O5 ha−1 (132.2 kg grain kg−1 P2O5). It can be concluded that dual applications of biofertilizers [Mesorhizobium + RB-1 (Pseudomonas argentinensis)] and 25 kg P2O5 ha−1 improve nutrient uptake by the crop and build up of nutrients in the soil after the harvest of the crop.

Effects of biofertilizers, lime and inorganic NPSB fertilizers on nodulation, growth and yield of soybean (Glycine max L. Merrill) in Assosa Zone, Western Ethiopia

The productivity of soybean in Assosa Zone particularly in Assosa and Bambassi districts is very low due to poor soil fertility management practices which resulted in severe soil acidity and low N-fixing inoculant in the soil. Hence, this experiment was conducted during the main cropping season of 2019 and 2020 in Assosa and Bambassi districts to evaluate the effect of biofertilizers, lime, and inorganic NPSB fertilizers on nodulation, growth, and yield of soybean (Glycine max L. Merrill). Factorial combinations of four biofertilizer inoculants [without biofertilizer (B1), SB12 biofertilizer at the recommended rate of 500 g ha −1 (B2), MAR1495 biofertilizer at the recommended rate of 500 g ha−1 (B3) and SB12 plus MAR1495 biofertilizers at their recommended rates (B4); two lime rates named without lime (L1) and lime at 5 ton/ha (L2); two inorganic NPSB fertilizers NPSB at 9.5-23-3.5-0.05 (F1) and NPSB at 19-46-7-0.1 (F2) at their recommended rates for soybean] were laid out in a randomized complete block design (RCBD) with three replications. Effective nodules, leaf area index, and grain yield were collected and analyzed using SAS 9.1.3 version software. Results of the experiment showed that effective nodules per plant, leaf area index, and grain yield were affected by biofertilizers, lime, inorganic fertilizers, and their interactions significantly (p < 0.01) at both locations and years. Finally, the interaction of SB12 + MAR1495, lime at 5 t/ha and NPSB at 19-46-7-0.1, and the interaction of SB12, lime at 5 t/ha and NPSB at 19-46-7-0.1 gave the maximum grain yield at Assosa and Bambassi, respectively.

Biofertilizers, types, Benefits and their role in improving the growth of fruit trees and vegetative plants (Review)

Plant development, kinds, and benefits of bio-fertilizer inoculation will be discussed in this paper, Studies have shown that using bio-fertilizers increases the growth of the host plant, as well as improving the root growth and increasing the root system area, which leads to an increase in water and nutrient absorption. Also, the use of biological fertilizers helps reduce the toxicity in plant products as a result of a decrease in chemical fertilizers, In addition to increasing plant resistance to stress conditions like salt, drought, and temperature, bio-fertilizers can also increase soil fertility by contributing to soil aggregation and increasing the activity of soil enzymes like dehydrogenase, phosphatase, and urease, for example. These enzymes help plants be stronger against stress conditions like salt, drought, and temperature

Application of Bio-Fertilizers Improves Forage Quantity and Quality of Sorghum (Sorghum bicolor L.) Intercropped with Soybean (Glycine max L.).

In recent years, application of bio-fertilizers (BFs) in intercropping systems has become known as one of the main sustainable and eco-friendly strategies for improving the quantity and quality of forage crops. In order to evaluate the forage quantity and quality of sorghum intercropped with soybean, a two-year field experiment was carried out as factorial based on a randomized complete blocks design (RCBD) with three replications. The first factor was different cropping patterns including soybean monocultures with densities of 40 and 50 plants m-2 (G40 and G50), sorghum monocultures with densities of 10 and 15 plants m-2 (S10 and S15) and intercropping of two plants with the mentioned densities. The second factor was non-application (control) and application of bio-fertilizers. The results demonstrated that the highest dry forage yield of sorghum (21.22 t ha-1) was obtained in monoculture conditions with density of 15 plants m-2 and inoculation with bio-fertilizer (S15+BF). The maximum crude protein (CP = 149.6 g kg-1 DM), ash (113.2 g kg-1 DM), water soluble carbohydrates (WSC = 251.16 g kg-1 DM), dry matter intake (DMI = 26.83 g kg-1 of body weight), digestible dry matter (DDM = 668.01 g kg-1 DM), total digestible nutrients (TDN = 680.42 g kg-1 DM), relative feed value (RFV = 142.98%) and net energy for lactation (NEL = 1.625 Mcal kg-1) were observed in the intercropping of S10G50 inoculated with BF. Interestingly, application of bio-fertilizers enhanced the content of CP, ash, WSC, DMI, DDM, TDN, RFV and NEL by 7.5, 8, 11.7, 3.6, 2.3, 12.3, 5.9 and 3.5% when compared with the control (non-application of bio-fertilizers). In all intercropping patterns, the total land equivalent ratio (LER) value was greater than one, representing the advantage of these cropping patterns in comparison with sorghum monoculture. The highest total LER was recorded in the intercropping of S15G40 and S10G50 following application of BF. Additionally, the highest monetary advantage index (MAI) was calculated in the intercropping of S15G40+BF. Generally, it can be concluded that the intercropping of S10G50 along with bio-fertilizer inoculation could be suggested as an eco-friendly strategy for improving the forage quantity and quality under low-input conditions.

Revitalizing sub-optimal drylands: Exploring the role of biofertilizers

Abstract Indonesia has a dry land area of 79.69%, with low soil productivity (physical, chemical, and biological), as well as uneven and unpredictable rainfall. The dryland potential is optimally utilized using biofertilizers that can produce microbes to increase soil fertility. This research aims to determine the effects of biofertilizers on dryland improvement and crop production. The study was conducted from February to May 2021 in Central Java, Indonesia. Using a randomized block design in peanut cultivation. Six biofertilizers (Controlled, Agrimeth, BioNutrient, Gliocompost, Agrimeth + BioNutrient, Agrimeth + BioNutrient + Gliocompost) were applied with four replications. The performance of each biofertilizer was assessed based on chemical soil parameters, soil microbe population, plant growth, and yields. The soil in the study area belonged to the Inceptisols group and exhibited moderately acidic pH, low organic carbon content, and low nitrogen levels. However, it had high potential and available phosphorus, as well as moderate potential and high available potassium. BioNutrient and Gliocompost increased available phosphate by 12 and 19%, respectively, due to the presence of Pseudomonas fluorescens and Aspergillus sp. Agrimeth influenced the population of Azospirillum (45–63%) and enhanced phosphate-solubilizing bacteria. Agrimeth + BioNutrient + Gliocompost promoted the growth of the Azospirillum and Trichoderma populations (17–18%), resulting in a 45.04% increase in profits. Biofertilizer inoculation positively affected peanut development, root nodule formation, and yield. This novelty showed the potential of biofertilizers in improving dryland conditions, increasing crop productivity, and contributing to sustainable agriculture in the long term.

Costeffective formulation of bio-fertilizer using agricultural residues as carriers and determination of shelflife of bio-fertilizer inoculants

Costeffective formulation of bio-fertilizer using agricultural residues as carriers and determination of shelflife of bio-fertilizer inoculants

Use of organic manures in combination with Azotobacter in strawberry: effect on growth, yield and quality attributes

The use of organic manures and biofertilizers as a nutrient source is a sustainable production method to improve crop yield and quality. A pot experiment was conducted during the year 2017–18 and 2018–19 at hi-tech greenhouse of the Department of Horticulture, Choudhary Charan Singh Haryana Agricultural University, Hissar, Haryana (India) with comprised of nine treatments, viz., Sand: FYM (3:1), Sand: FYM (2:1), Sand: Vermicompost (3:1), Sand: Vermicompost (2:1), Sand: FYM (3:1) + Azotobacter, Sand: FYM (2:1) + Azotobacter, Sand: Vermicompost (3:1) + Azotobacter, Sand: Vermicompost (2:1) + Azotobacter and Control (Sand), in a complete randomized design with five replications, over a two-growing seasons. Plants supplied with Sand: Vermicompost (2:1) + Azotobacter inoculation recorded higher plant growth, fruit yield, which was at par with Sand: Vermicompost (3:1) + Azotobacter inoculation than other combination. Maximum TSS, vitamin C and anthocyanin content were observed under the treatment combination of sand: vermicompost (2:1) + Azotobacter inoculation in comparison with other treatments. The results demonstrated that there will be a significant improvement in growth, yield and quality attributes of strawberry, when plats supplied with organic manure in combination of Azotobacter inoculation than without manure and biofertilizer inoculation.

Market potential study of NutrioTM biofertilizer for sugarcane and eggplant

NutrioTM is a new foliar spray biofertilizer inoculant containing endophytic bacteria that has been tested for improved yield of sugarcane. The product has also been proven effective for eggplant production. Although NutrioTM has already been in the market, it was still operating on a small-scale during the time this research was conducted. This PCAARRD-funded study looked into the market potential of NutrioTM as biofertilizer for sugarcane and eggplant. Specifically, this research aimed to a) define the potential market for NutrioTM, which was anchored on the sugarcane and eggplant commodity systems analyses, b) determine the level of awareness, perceptions/evaluations and willingness of the potential market to use/buy NutrioTM, c) estimate the market requirements for NutrioTM, d) identify market-related challenges toward the commercialization of NutrioTM and e) recommend pathways for the commercialization of Nutrio.TM Three provinces were randomly selected for each commodity, namely, Batangas, Iloilo and Negros Occidental (for sugarcane), and Pangasinan, Iloilo and Cagayan (for eggplant). A total of 148 farmer-respondents were interviewed. Key informant interviews and literature review were also conducted. Both qualitative and quantitative data analyses were employed. NutrioTM biofertilizer can be considered as a promising technology based on the responses of the sugarcane and eggplant farmers interviewed. Among 148 respondents, 134 (91%) were willing to buy the product. Establishing a new enterprise for manufacturing and selling of the technology of 435,870.56 kilograms of the product for both sugarcane and eggplant market would be profitable. Furthermore, distribution of the product through existing enterprises would also be profitable, with an additional potential income of PHP64,523,630.00 if product distributorship would be adopted. Some strategies to expand the business scale of NutrioTM, such as the improvement of product packaging, product demonstration and field testing and product label expansion as well as adoption of distributorship for the technology to realize its full potential were recommended.