Bioenergy Feedstock Research Articles

Harvest and nitrogen effects on bioenergy feedstock quality of grass‐legume mixtures on Conservation Reserve Program grasslands

AbstractPerennial grass mixtures established on Conservation Reserve Program (CRP) lands can be an important source of feedstock for bioenergy production. This study aimed to evaluate management practices for optimizing the quality of bioenergy feedstock and stand persistence of grass‐legume mixtures under diverse environments. A 5‐year field study (2008–2012) was conducted to assess the effects of two harvest timings (at anthesis vs after complete senescence) and three nitrogen (N) rates (0, 56, 112 kg N ha−1) on biomass chemical compositions (i.e., cell wall components, ash, volatiles, total carbon, and N contents) and the feedstock energy potential, examined by the theoretical ethanol yield (TEY) and the total TEY (i.e., the product of biomass yield and TEY, L ha−1), of cool‐season mixtures in Georgia and Missouri and a warm‐season mixture in Kansas. The canonical correlation analysis (CCA) was used to investigate the effect of vegetative species transitions on feedstock quality. Although environmental variations (mainly precipitation) greatly influenced the management effect on chemical compositions, the delayed harvest after senescence generally improved feedstock quality. In particular, the overall cell wall concentrations and TEY of the warm‐season mixtures increased by approximately 7%. Additional N supplies improved the total TEY of both mixtures by ~1.6–4.2 L ha−1 per 1.0 kg N ha−1 input but likely lowered the feedstock quality, particularly for the cool‐season mixture. The cell wall concentrations of cool‐season mixture reduced by approximately 3%–6%. The CCA results indicated that the increased legume compositions (under low N input) likely enhanced lignin but reduced ash concentrations. This field research demonstrated that with proper management, grass‐legume mixtures on CRP lands can provide high‐quality feedstock for bioenergy productions.

Ten new high-quality genome assemblies for diverse bioenergy sorghum genotypes.

Sorghum (Sorghum bicolor (L.) Moench) is an agriculturally and economically important staple crop that has immense potential as a bioenergy feedstock due to its relatively high productivity on marginal lands. To capitalize on and further improve sorghum as a potential source of sustainable biofuel, it is essential to understand the genomic mechanisms underlying complex traits related to yield, composition, and environmental adaptations. Expanding on a recently developed mapping population, we generated de novo genome assemblies for 10 parental genotypes from this population and identified a comprehensive set of over 24 thousand large structural variants (SVs) and over 10.5 million single nucleotide polymorphisms (SNPs). We show that SVs and nonsynonymous SNPs are enriched in different gene categories, emphasizing the need for long read sequencing in crop species to identify novel variation. Furthermore, we highlight SVs and SNPs occurring in genes and pathways with known associations to critical bioenergy-related phenotypes and characterize the landscape of genetic differences between sweet and cellulosic genotypes. These resources can be integrated into both ongoing and future mapping and trait discovery for sorghum and its myriad uses including food, feed, bioenergy, and increasingly as a carbon dioxide removal mechanism.

Uncertain effectiveness of Miscanthus bioenergy expansion for climate change mitigation explored using land surface, agronomic and integrated assessment models

AbstractLarge‐scale bioenergy plays a key role in climate change mitigation scenarios, but its efficacy is uncertain. This study aims to quantify that uncertainty by contrasting the results of three different types of models under the same mitigation scenario (RCP2.6‐SSP2), consistent with a 2°C temperature target. This analysis focuses on a single bioenergy feedstock, Miscanthus × giganteus, and contrasts projections for its yields and environmental effects from an integrated assessment model (IMAGE), a land surface and dynamic global vegetation model tailored to Miscanthus bioenergy (JULES) and a bioenergy crop model (MiscanFor). Under the present climate, JULES, IMAGE and MiscanFor capture the observed magnitude and variability in Miscanthus yields across Europe; yet in the tropics JULES and IMAGE predict high yields, whereas MiscanFor predicts widespread drought‐related diebacks. 2040–2049 projections show there is a rapid scale up of over 200 Mha bioenergy cropping area in the tropics. Resulting biomass yield ranges from 12 (MiscanFor) to 39 (JULES) Gt dry matter over that decade. Change in soil carbon ranges from +0.7 Pg C (MiscanFor) to −2.8 Pg C (JULES), depending on preceding land cover and soil carbon.2090–99 projections show large‐scale biomass energy with carbon capture and storage (BECCS) is projected in Europe. The models agree that <2°C global warming will increase yields in the higher latitudes, but drought stress in the Mediterranean region could produce low yields (MiscanFor), and significant losses of soil carbon (JULES and IMAGE). These results highlight the uncertainty in rapidly scaling‐up biomass energy supply, especially in dry tropical climates and in regions where future climate change could result in drier conditions. This has important policy implications—because prominently used scenarios to limit warming to ‘well below 2°C’ (including the one explored here) depend upon its effectiveness.

Life-Cycle Greenhouse Gas Emissions from Forest Bioenergy Production at Combined Heat and Power Projects in Nova Scotia, Canada

Abstract Forest bioenergy production can represent a renewable energy supply while benefiting the forest sector. However, greenhouse gas (GHG) reductions are often not immediate. The point of carbon parity where bioenergy starts delivering GHG benefits may be years to decades in the future. This study examined the life-cycle emissions associated with bioenergy production at combined heat-and-power (CHP) projects in Nova Scotia, Canada. We examined the effects and sensitivities of different feedstock mixes of chips from harvested roundwood and mill residues, the implementation of intensive and extensive silviculture strategies, and different market/supply-chain assumptions around additionality and product substitution. We found contrasting GHG outcomes for bioenergy, depending largely on additionality assumptions and biomass type. When primary biomass (roundwood) was used as the feedstock type, carbon parity was achieved within four to nine years when pulp and paper products were substituted, whereas carbon parity was achieved in 86–100 years or longer when biomass harvests were additional. Net GHG benefits were achieved in 10 years with the use of secondary biomass (mill residues) as the bioenergy feedstock, although they were delayed when at lower energy conversion efficiencies. Adoption of more intensive silvicultural practices (plantations) reduced the time to carbon parity because of increased yields, although uncertainties in long-term soil carbon storage exist. Study Implications: Our analysis shows that the use of forest biomass in local CHP facilities can deliver GHG benefits in the short term but there is substantial variability. Carbon parity times were the longest with the use of additional primary biomass feedstocks (i.e., roundwood) but were substantially reduced when biomass harvests substituted harvests for pulp and paper products and when secondary biomass (i.e., mill residues) was used. This study highlights the nuance of different forest management dimensions (e.g., silviculture) while also presenting novel findings on the importance of assumptions around biomass harvesting being additional to current practices or a substitution for declines in traditional forest products.

In the direction of a sustainable future: A Comprehensive Review of Evolution, Environmental Impacts, and Future Prospects of Bioenergy

As global focus sharpens on carbon emissions and environmental protection; the pursuit of sustainable development permeates every sector. Against the backdrop of increasing fossil fuel prices and relentless energy demand, the exploration of clean energy has become paramount. This paper presents a comprehensive review of bioenergy. It introduces the concept and underscores its importance, tracing the historical stages and accomplishments in its development. The paper explicates different types of bioenergy and their chemical operating principles. The integral system of bioenergy is also evaluated, focusing on crucial components: bioenergy feedstocks, processing technologies, transport process, storage, and grid integration. The paper concludes with an assessment of bioenergy's economic and environmental impacts, considering market dynamics and future prospects, and suggests potential mitigation measures against its environmental repercussions.

Variable lignin structure revealed in Populus leaves.

Lignin has long been a trait of interest, especially in bioenergy feedstocks such as Populus. While the stem lignin of Populus is well studied, foliar lignin has received significantly less consideration. To this end, leaves from 11 field grown, natural variant Populus trichocarpa genotypes were investigated by NMR, FTIR, and GC-MS. Five of these genotypes were sufficiently irrigated, and the other six genotypes were irrigated at a reduced rate (59% of the potential evapotranspiration for the site) to induce drought treatment. Analysis by HSQC NMR revealed highly variable lignin structure among the samples, especially for the syringyl/guaiacyl (S/G) ratio, which ranged from 0.52-11.9. Appreciable levels of a condensed syringyl lignin structure were observed in most samples. The same genotype subjected to different treatments exhibited similar levels of condensed syringyl lignin, suggesting this was not a response to stress. A cross peak of δC/δH 74.6/5.03, consistent with the erythro form of the β-O-4 linkage, was observed in genotypes where significant syringyl units were present. Principle component analysis revealed that FTIR absorbances associated with syringyl units (830 cm-1, 1317 cm-1) greatly contributed to variability between samples. Additionally, the ratio of 830/1230 cm-1 peak intensities were reasonably correlated (p-value < 0.05) with the S/G ratio determined by NMR. Analysis by GC-MS revealed significant variability of secondary metabolites such as tremuloidin, trichocarpin, and salicortin. Additionally, salicin derivatives were found to be well correlated with NMR results, which has been previously hypothesized. These results highlight previously unexplored nuance and variability associated with foliage tissue of poplar.

Cropping system drives microbial community response to simulated climate change and plant inputs

The soil microbiome’s role in regulating biogeochemical processing is critical to the cycling and storage of soil organic carbon (C). The function of the microbiome under different land management uses has become a focal area of research due to the interest in managing soil C to mitigate climate change. This study investigates the structural and functional response of soil microbiomes from annual monoculture (corn [<i>Zea mays</i> L.]) and perennial diversified (prairie) cropping systems, both under no-till management for bioenergy production. We used a full factorial soil incubation study to understand the influence of temperature and moisture on microbial C decomposition in these soils, with and without addition of cellulose as a model plant residue. Overall, perennial prairie soil supported distinct microbiomes with more diverse prokaryotic and fungal communities compared to annual corn soil. The less diverse corn microbiome was sensitive to the addition of C, resulting in significantly higher respiration compared to prairie, and this increased respiration was amplified under warmer temperatures. In contrast to C loss from the corn soil as carbon dioxide (CO₂), prairie soil had significantly higher extracellular enzyme activities and small increases in microbial biomass, illustrating cropping system-specific tradeoffs between microbial C allocation. Specific community structure shifts occurred with added cellulose, where fast-growing, motile decomposers became more abundant under wet conditions, while a small subset of fungi dominated under dry conditions. These differential responses of fungi and bacteria reflect microbial traits important for accessing substrates like plant residues. These changes in community structure due to moisture and cellulose amendment were not necessarily reflected in community function, as potential enzyme activities of most hydrolases were insensitive to temperature and C amendment on this short time scale. Lower respiration occurred in prairie compared to corn soil in response to increased available C and temperature, indicating a more resistant prairie microbiome that may be beneficial when confronted with climate change. These findings support deploying perennial and diversified systems in place of annual monocultures as bioenergy feedstocks, cover crops, buffer strips, or urban greenspaces as part of a land management strategy and highlight the importance of microbial activity in developing sustainable agroecosystems.

Biofuels from Renewable Sources, a Potential Option for Biodiesel Production.

Ever-increasing population growth that demands more energy produces tremendous pressure on natural energy reserves such as coal and petroleum, causing their depletion. Climate prediction models predict that drought events will be more intense during the 21st century affecting agricultural productivity. The renewable energy needs in the global energy supply must stabilize surface temperature rise to 1.5 °C compared to pre-industrial values. To address the global climate issue and higher energy demand without depleting fossil reserves, growing bioenergy feedstock as the potential resource for biodiesel production could be a viable alternative. The interest in growing biofuels for biodiesel production has increased due to its potential benefits over fossil fuels and the flexibility of feedstocks. Therefore, this review article focuses on different biofuels and biomass resources for biodiesel production, their properties, procedure, factors affecting biodiesel production, different catalysts used, and greenhouse gas emissions from biodiesel production.

Overexpression of PvBiP2 improved biomass yield and cadmium tolerance in switchgrass (Panicum virgatum L.)

Switchgrass (Panicum virgatum L.), the prime bioenergy feedstock crop, is one ideal candidate for phytoremediation of cadmium (Cd). The absorption of Cd imposes severe endoplasmic reticulum (ER)-stress in plants. ER chaperone binding proteins (BiPs) are important modulators in ER-stress responses. The objective of this study was to characterize one Cd-responsive BiP gene, PvBiP2, in switchgrass for its roles in Cd tolerance and plant growth. PvBiP2 was up-regulated by Cd and the ER-stress inducer, dithiothreitol (DTT) and could be trans-activated by one Cd-responsive heat shock transcription factor PvHsfA4. Overexpression of PvBiP2 in switchgrass significantly increased its plant growth with higher height, stem diameter, leaf width, internode length, and tiller numbers than those of the wildtype (WT) plants under non-stress conditions. After 30 days of Cd treatment, the PvBiP2 over-expression transgenic lines showed 40–45% higher dry biomass accumulation with net photosynthesis rate (Pn), but lower electrolyte leakage (EL), malondialdehyde (MDA), and glutathione (GSH) levels than WT. Moreover, over-expressing PvBiP2 led to ∼90–140% Cd accumulation in plants but 46–57% lower Cd translocation rates to shoots. Together, the PvHSFA4-PvBiP2 module acted as positive regulators in plant Cd tolerance, and over-expressing PvBiP2 promoted plant vegetative growth as well as Cd tolerance making it an ideal molecular target for genetic improvement in switchgrass in the future.

An analysis of the social and private return to land use change from agriculture to renewable energy production in Ireland

Increased use of renewable energy is a major part of Ireland's plans to reduce greenhouse gas (GHG) emissions, lessen reliance on fossil fuels, and enable sustainable economic growth. Two forms of renewable energy interesting Irish policymakers are bioenergy and solar PV. To scale up these technologies, land will be required to grow bioenergy feedstocks and erect ground mount solar PV arrays. A micro, farm-based approach is utilised to generate private returns from renewable energy production, and agriculture, adjusted for the cost of GHGs. This considers both the cost to society of increasing GHG emissions and the benefit of reducing emissions.This paper compares income that could be derived from renewable energy production with average agricultural income from the main farm systems in Ireland. The private (market and subsidy) and social (GHG) return from substituting renewable energy sources for the existing agricultural land use on 1 ha is considered. We also use input-output modelling to generate output multipliers for renewable energy technologies: biogas, biomethane, short rotation coppice (SRC) willow, and solar PV.The results show that the market return from growing 1 ha of grass silage and SRC willow is less than for all the main farming systems. When the social value of the feedstock is considered by putting a monetary value on displaced GHG emissions, producing grass silage and willow biomass can provide a better return to farmers than cattle and sheep farming. Due to the high land rental amounts available to farmers from solar companies, the market and social return is higher for using land to erect solar PV than any of the main agricultural activities. Results from the output multiplier analysis show that biomethane production has the highest multiplier while solar PV has the lowest.

Edaphic controls of soil organic carbon in tropical agricultural landscapes

Predicting soil organic carbon (SOC) is problematic in tropical soils because mechanisms of SOC (de)stabilization are not resolved. We aimed to identify such storage mechanisms in a tropical soil landscape constrained by 100 years of similar soil inputs and agricultural disturbance under the production of sugarcane, a C4 grass and bioenergy feedstock. We measured soil physicochemical parameters, SOC concentration, and SOC dynamics by soil horizon to one meter to identify soil parameters that can predict SOC outcomes. Applying correlative analyses, linear mixed model (LMM) regression, model selection by AICc, and hierarchical clustering we found that slow moving SOC was related to many soil parameters, while the fastest moving SOC was only related to soil surface charge. Our models explained 78–79%, 51–57%, 7–8% of variance in SOC concentration, slow pool decay, and fast pool decay, respectively. Top SOC predictors were roots, the ratio of organo-complexed iron (Fe) to aluminum (Al), water stable aggregates (WSagg), and cation exchange capacity (CEC). Using hierarchical clustering we also assessed SOC predictors across gradients of depth and rainfall with strong reductions in Roots, SOC, and slow pool decay associated with increasing depth, while increased rainfall was associated with increased Clay and WSagg and reduced CEC in surface soils. Increased negative surface charge, water stable aggregation, organo-Fe complexation, and root inputs were key SOC protection mechanisms despite high soil disturbance. Further development of these relationships is expected to improve understanding of SOC storage mechanisms and outcomes in similar tropical agricultural soils globally.

Towards multifunctional landscapes coupling low carbon feed and bioenergy production with restorative agriculture: Economic deployment potential of grass‐based biorefineries

Grass-based biomass from grasslands can be used as feedstock in green biorefineries (GBs) that produce a range of bio-products. In addition, adjustments made as part of crop rotation to increase areas under temporary grasslands can yield benefits such as carbon sequestration, increased soil productivity, reduced eutrophication, and reduced need for pesticides. A flexible modeling framework is developed to analyze the deployment options for GBs that use grass-clover to produce protein feed and feedstock for bioenergy. The focus was on optimal deployment, considering system configuration and operation, as well as land use changes designed to increase grass-clover cultivation on cropland. A case study involving 17 counties in Sweden showed that deployment of GB systems could support biomethane and protein feed production corresponding to 5%–60% and 13%–154%, respectively, of biomethane and soybean feed imports to Sweden in Year 2020. This article is protected by copyright. All rights reserved.

Doubling protected land area may be inefficient at preserving the extent of undeveloped land and could cause substantial regional shifts in land use

AbstractProjection of land use and land‐cover change is highly uncertain yet drives critical estimates of carbon emissions, climate change, and food and bioenergy production. We use new, spatially explicit land availability data in conjunction with a model sensitivity analysis to estimate the effects of additional land protection on land use and land cover. The land availability data include protected land and agricultural suitability and is incorporated into the Moirai land data system for initializing the Global Change Analysis Model. Overall, decreasing land availability is relatively inefficient at preserving undeveloped land while having considerable regional land‐use impacts. Current amounts of protected area have little effect on land and crop production estimates, but including the spatial distribution of unsuitable (i.e., unavailable) land dramatically shifts bioenergy production from high northern latitudes to the rest of the world, compared with uniform availability. This highlights the importance of spatial heterogeneity in understanding and managing land change. Approximately doubling the current protected area to emulate a 30% protected area target may avoid land conversion by 2050 of less than half the newly protected extent while reducing bioenergy feedstock land by 10.4% and cropland and grazed pasture by over 3%. Regional bioenergy land may be reduced (increased) by up to 46% (36%), cropland reduced by up to 61%, pasture reduced by up to 100%, and harvested forest reduced by up to 35%. Only a few regions show notable gains in some undeveloped land types of up to 36%. Half of the regions can reach the target using only unsuitable land, which would minimize impacts on agriculture but may not meet conservation goals. Rather than focusing on an area target, a more robust approach may be to carefully select newly protected land to meet well‐defined conservation goals while minimizing impacts to agriculture.

Effective fractionation of microalgae biomass as an initial step for its utilization as a bioenergy feedstock

• S. obliquus was effectively fractionated by mild (un)catalyzed hydrothermal processes. • Soluble sugars were mainly present as soluble oligosaccharides. • Protein was mainly retained in the solid fraction. • Dilute acid hydrolysis allowed higher oligosaccharides yield at mild conditions. • Co-production of added-value oligosaccharides in marine biorefineries. Scenedesmus obliquus , a biotechnologically recognized microalga, was grown in 70 L vertical photobioreactors using non-supplemented secondary brewery wastewater as a culture medium. Upon collection, by mechanical means, the cells were subjected to hydrothermal (autohydrolysis) and dilute acid hydrolysis (0.5% sulfuric acid) pre-treatments were carried out using pressure micro-reactors under isothermal conditions up to 300 min. Both processes enabled a high recovery of soluble sugars (∼50%) that were, in a great majority, present in the added-value oligomeric form (92% and 90.5% for autohydrolysis and dilute acid hydrolysis, respectively). Protein solubilization also presented relevant yields (35% removal), with dilute acid hydrolysis allowing both higher oligosaccharides and protein productivities at a milder temperature. As compared to the current whole microalgae biomass-based upgrade strategies, the use of these mild processes is extremely promising, as they will enable the future co-production of added-value oligosaccharides, and protein, which can be relevant co-products of a biofuels-based biorefinery.

Bioremediation of ethanol wash by microalgae and generation of bioenergy feedstock

Bioremediation of ethanol wash by microalgae and generation of bioenergy feedstock

Critical evaluation of novel applications of aquatic weed Azolla as a sustainable feedstock for deriving bioenergy and feed supplement

Azolla is often considered an invasive weed because of the rapid colonization on water surfaces. It is a free-floating aquatic fern, known for its N fixing potential, short doubling time, and bioremediation potential. It has several applications as green manure, N biofertilizer, cattle, chicken, and fish feed supplements because of its high protein content. It can grow on wastewater improving its quality. Azolla biomass thus produced can be utilized for energy production. It has been used for the production of biogas. Recently, its bio-oil potential has been studied. Azolla biomass has been used for biodiesel, biogas, and biohydrogen production. The exploitation of Azolla biomass as feedstock for bioenergy needs critical evaluation.

Integrating Analytical Hierarchical Process and Network Optimization Model to Support Decision-Making on Biomass Terminal Selection

Forest biomass is an appealing bioenergy feedstock due its renewability, availability and potential to stimulate local economies. It is, however, voluminous, with heterogenous fuel characteristics and uncertainties in its supply. The feasibility of a bioenergy facility is contingent on a secure supply of uniform feedstock; a terminal in the supply chain can be useful in this regard. Biomass can be treated in the terminal to meet quality specifications and stored to overcome seasonality and supply disruptions. Nonetheless, such terminals require a significant capital investment; thus, the decision to use a terminal needs to be made judiciously. The decision process must account for a diverse set of factors that influence the terminal’s effectiveness. These include both quantitative and qualitative factors. The objective of this study is to develop a multi-criteria decision-making framework that takes quantitative and qualitative factors into consideration while selecting a terminal. The framework consists of analytical hierarchy process to analyze qualitative information, and a mixed-integer programming model to evaluate quantitative information including fuel quality (moisture content and thermal value). This hybrid framework was implemented in a case study. It proved to be an effective tool for identifying terminals with the highest potential to generate value for the bioenergy supply chain.

The comprehensive characterization of Prosopis juliflora pods as a potential bioenergy feedstock

The production of renewable and sustainable biofuels using inevitable wastes is a promising alternative to the alarming depletion of fossil fuels. Significantly, the sustainable biorefinery of lignocellulosic waste, as an alternative fuel source, is a prognosticating approach to tackle many agricultural/forestry residues and offers a circular economy as well as environmental benefits. But, the heterogeneity of lignocellulosic biomass is one of the major bottlenecks in lignocellulosic biorefinery. Thus the characterization of lignocellulosic biomass is essential to understanding the feedstock's nature, composition and suitability for biofuel production. The present study taps evergreen spiny non-edible pods of Prosopis juliflora (Pj) as an energy feedstock. Proximate, ultimate and biochemical characterization of Pj pods were conducted, and thermal behaviour and calorific values were determined. Cellulose and hemicellulose were isolated and characterized by reliable methods. The overall characterization has revealed the Pj pods as a potential feedstock for bioenergy. The collected Pj pods contain (% w/w) moisture 7.89 ± 0.002, volatile matter 87.67 ± 0.002, ash 0.21 ± 0.002, fixed carbon 4.23 ± 0.002 with a calorific value of 17.62 kg/MJ. The CHNS content was (w/w %) carbon 41.77, nitrogen 3.58, sulfur 26.3 and hydrogen 6.55. The biochemical composition analysis yields (% w/w) on a dry basis; cellulose 26.6 ± 0.18, hemicellulose 30.86 ± 0.27, lignin 4.71 ± 0.12, protein 11.63 ± 0.12 and starch 1.1 ± 0.06 and extractives 30.56 ± 0.008. The isolated cellulose and hemicellulose were analyzed and confirmed by CP/MAS &1H NMR, FTIR, TG-DSC, SEM, XRD, and TGA. The present results revealed that the tested biomass, Prosopis juliflora, could be used as a feedstock in biorefinery for bioenergy.

Climate mitigation potential and soil microbial response of cyanobacteria‐fertilized bioenergy crops in a cool semi‐arid cropland

AbstractBioenergy carbon capture and storage (BECCS) systems can serve as decarbonization pathways for climate mitigation. Perennial grasses are a promising second‐generation lignocellulosic bioenergy feedstock for BECCS expansion, but optimizing their sustainability, productivity, and climate mitigation potential requires an evaluation of how nitrogen (N) fertilizer strategies interact with greenhouse gas (GHG) and soil organic carbon (SOC) dynamics. Furthermore, crop and fertilizer choice can affect the soil microbiome which is critical to soil organic matter turnover, nutrient cycling, and sustaining crop productivity but these feedbacks are poorly understood due to the paucity of data from certain agroecosystems. Here, we examine the climate mitigation potential and soil microbiome response to establishing two functionally different perennial grasses, switchgrass (Panicum virgatum, C4) and tall wheatgrass (Thinopyrum ponticum, C3), in a cool semi‐arid agroecosystem under two fertilizer applications, a novel cyanobacterial biofertilizer (CBF) and urea. We find that in contrast to the C4 grass, the C3 grass achieved 98% greater productivity and had a higher N use efficiency when fertilized. For both crops, the CBF produced the same biomass enhancement as urea. Non‐CO2 GHG fluxes across all treatments were low and we observed a 3‐year net loss of SOC under the C4 crop and a net gain under the C3 crop at a 0–30 cm soil depth regardless of fertilization. Finally, we detected crop‐specific changes in the soil microbiome, including an increased relative abundance of arbuscular mycorrhizal fungi under the C3, and potentially pathogenic fungi in the C4 grass. Taken together, these findings highlight the potential of CBF‐fertilized C3 crops as a second‐generation bioenergy feedstock in semi‐arid regions as a part of a climate mitigation strategy.

Are we ready to cultivate cactus pear as a bioenergy feedstock in semi-arid land? A review on biofuels production

ISHS X International Congress on Cactus Pear and Cochineal: Cactus - the New Green Revolution in Drylands Are we ready to cultivate cactus pear as a bioenergy feedstock in semi-arid land? A review on biofuels production