Biodiversity Research Articles

The main causative factor of increased mortality rate in experimental rat

Rats are still often used in vital areas of biological study. Animal management, behavior, compliance and technical processes directly relevant to research projects are just a few of the many duties placed on the shoulders of those conducting such studies. This study was designed to fill a gap in the literature by providing a rapid reference source for researchers and animal care takers responsible for the care and/or usage of rats in research settings, since no prior study has detailed the death rate of experimental rats. A total of 103 adult wister albino rats, aged (12-15) weeks, were included in this study. The rats were subjected to orthodontic treatment using a metal spring coil measuring 6 mm in length, with force of 100 gm. to induce tooth movement. The experimental study involved a total of 56 rats, with 47 rats being excluded from the study due to mortality during the course of the study. The total death rate was determined. The greatest fatality rate was found to be 32%, and it was caused by rats related factors. The anesthetic death rate was 23%. The mortality rate in a controlled laboratory environment was 26%. The lowest cause of death rate was found to be the environmental factors at 19%. To maintain the reliability and repeatability of study results, it is crucial to implement a health monitoring programme for the experimental rat, whose death rate may be significantly impacted by factors such as biological diversity, stress, and an exhausted mechanism.

Pathogenic Rickettsia spp. as emerging models for bacterial biology.

Our understanding of free-living bacterial models like Escherichia coli far outpaces that of obligate intracellular bacteria, which cannot be cultured axenically. All obligate intracellular bacteria are host-associated, and many cause serious human diseases. Their constant exposure to the distinct biochemical niche of the host has driven the evolution of numerous specialized bacteriological and genetic adaptations, as well as innovative molecular mechanisms of infection. Here, we review the history and use of pathogenic Rickettsia species, which cause an array of vector-borne vascular illnesses, as model systems to probe microbial biology. Although many challenges remain in our studies of these organisms, the rich pathogenic and biological diversity of Rickettsia spp. constitutes a unique backdrop to investigate how microbes survive and thrive in host and vector cells. We take a bacterial-focused perspective and highlight emerging insights that relate to new host-pathogen interactions, bacterial physiology, and evolution. The transformation of Rickettsia spp. from pathogens to models demonstrates how recalcitrant microbes may be leveraged in the lab to tap unmined bacterial diversity for new discoveries. Rickettsia spp. hold great promise as model systems not only to understand other obligate intracellular pathogens but also to discover new biology across and beyond bacteria.

How does vector diversity influence the transmission efficiency of yellow dwarf virus? Perspectives from a review

AbstractCereals are some of the most important global crops that contribute directly and indirectly to the production of food for human consumption. Cereal aphids can cause significant damage to wheat, barley and oats, particularly via the transmission of plant viruses that cause devastating plant diseases, such as yellow dwarf disease. High levels of yellow dwarf disease can result in yield losses of around 20%, rising to 80% if infection is severe. Yellow dwarf disease is caused by multiple viruses, including viruses within the families Tombusviridae and Solemoviridae. These include yellow dwarf virus species within the genus Luteovirus (Barley yellow dwarf virus) and Polerovirus (Cereal yellow dwarf virus, Wheat yellow dwarf virus, Maize yellow dwarf virus). Some yellow dwarf virus species are primarily vectored by one aphid species whereas others can be transmitted by multiple vectors. Biological diversity within a given vector species (e.g., genotype, biotype) can influence virus transmission efficiency. However, it is unclear what biological factors drive this variation within a given vector species. Understanding how biological variation in vector populations influences virus transmission efficiency can help to identify biological traits that underpin successful transmission in competent vector populations. Here, the available literature on yellow dwarf virus transmission efficiency is synthesized and significant variation in yellow dwarf virus transmission efficiency is detected between different populations for several vector species. Three biological mechanisms that potentially underpin this variation are proposed.

Role of Rare Species on Phytoplankton Size–Abundance Relationships and Size Structure across Different Biogeographical Areas

Phytoplankton guilds are commonly characterised by dominance effects, while the main contribution to biological diversity is given by rare species. Here, we analysed the influence of rare species on taxonomic and functional diversity, which is described by taxa richness and composition, cell size, and size–abundance relationships in phytoplankton guilds. We explore these relationships at global and regional scales by analysing phytoplankton guilds from five biogeographical regions: the Northern Atlantic Ocean (Scotland), the South-Western Atlantic Ocean (Brazil), the South-Western Pacific Ocean (Australia), the Indo-Pacific Ocean (Maldives), and the Mediterranean Sea (Greece and Turkey). We have comparatively analysed the phytoplankton taxonomic diversity of the whole dataset and with the datasets obtained by progressively subtracting taxa occurring in the last 1%, 5%, 10%, and 25% of both numerical abundance and overall biomass. Globally, 306 taxa were identified across five ecoregions with only 27 taxa accounting for 75% of overall numerical abundance and biomass; almost 50% of taxa were lost on every step. The removal of 1% of most rare taxa significantly affected the phytoplankton size–abundance relationships and body-size structure, strongly impacting on small taxa. The progressive removal of additional rare taxa did not further affect phytoplankton size–abundance relationships and size structure.

Patterns, processes and conservation management consequences of intraspecific diversity, illustrated by fishes from recently glaciated lakes

Abstract Patterns in phenotypic and genotypic diversity within many species are becoming increasingly apparent, although there remain many species for which such patterns have yet to be described adequately. Fishes from recently glaciated ecosystems are likely to be particularly rich in intraspecific diversity, yet current conservation management strategies are, in many parts of the world, particularly in Europe, conventionally and overwhelmingly focused on species, regardless of competing species concepts, and appropriate policies for managing diversity at a sub‐specific level still have to be developed. Occasional attempts to protect certain vulnerable ecotypes and proposed alternative units of conservation (e.g. ‘Pragmatic Species’ or ‘Evolutionarily Significant Units’) reinforce the conventional primacy of contemporary expressed patterns of variation. Intraspecific phenotypic and genotypic patterns are ultimately the result of complex processes of divergence; conservation approaches that focus on the products of evolution largely ignore the processes that generate and maintain those patterns. Policies that acknowledge the continuation of evolution, the derivation of novel diversity over often very short time spans and the role of environment in initiating and perpetuating these processes are poorly integrated into management strategy. To address possible deficits, where intraspecific diversity is not addressed in management practice, we believe it to be important first to characterize hidden genetic and phenotypic diversity, which may intimate eco‐evolutionary processes, initially among species of high conservation status. A second step should be to use an approach to intraspecific diversity that illuminates the ultimate processes and mechanisms that bring about that diversity, which also concedes the central role of the environment and affords adequate protection to the ecosystems that drive these processes, such as the United Nations Convention on Biological Diversity (CBD) Ecosystems approach.

THE CONVENTION ON BIOLOGICAL DIVERSITY AND ECOLOGICAL PROTECTION-RELATED LAWS AND POLICIES IN GHANA

This study sets out to examine the implementation of the Convention on Biological Diversity (CBD) in Ghana. A key objective of the study was to understand how the country has attempted to achieve sustainable development while reducing the risk to biodiversity and environmental pollution given its abundant natural resources. In an effort to achieve this and other related objectives of the study, the researcher adopted a qualitative approach to research which enabled him to gather relevant information in non-numerical format. The study consequently finds that though the country has introduced several legislations and policies to ensure ecological protection, several challenges still remain. Indeed, in Ghana today, attempting to ensure the functionality, orderliness and the usefulness of ecological protection policies such as those aimed at the effective implementation of the CBD necessarily requires that such policies have firm legitimacy among the local norms; legality alone is not enough. It is in view of this that the study advocates for a balanced obligation.

Phylogenetic, morphological and niche differentiation unveil new species limits for the big brown bat (Eptesicus fuscus).

Phylogeographic accounts of mammals across fragmented landscapes show high levels of genetic, morphological and ecological variation. The big brown bat (Eptesicus fuscus) widely spans mainland landmasses from Canada to Ecuador and Colombia, and the insular Caribbean through The Bahamas and Greater and Lesser Antilles. Given the distribution of E. fuscus, we hypothesized that insular lineages could represent a different species aided by isolation. We assessed species limits by capitalizing on available mitochondrial and genomic data. Novel morphological and spatial datasets were produced to examine limits phenotypically and whether ecological niches could be associated with differences between groups. Phylogenetics strongly supported the Caribbean as unique compared to the mainland. Genomic data indicated high levels of genetic structure within the Caribbean and no detectable admixture of the Caribbean with continental lineages. Similarly, the Caribbean group shows high phenotypic disparity, and niche models revealed differences in habitat suitability between groups, concordant with the phylogenetic results. This study uncovered signals of divergence supporting the Caribbean clade of E. fuscus as unique through an integrative framework. We endorse re-evaluating the taxonomic status of Caribbean big brown bats as Eptesicus dutertreus. This recognition can help promote local conservation plans for insular lineages of big brown bats.

Impact and control of feral cats preying on wandering albatrosses: Insights from a field experiment

AbstractInvasive alien species are a major threat to seabird species, and the number of impacted species is still increasing. A recent study revealed for the first time that feral cats predated a large albatross species and that without cat control, some albatross populations would markedly decline. We examined this new predator–prey system by individually monitoring known‐age wandering albatross chicks with camera traps in a colony experimentally divided into zones with and without cat control. Our design allowed us to investigate how cat control influenced cat abundance and how this in turn influenced the probability for a chick to be predated by a cat. After cat controls, cat abundance was lower in controlled zones than in uncontrolled zones, while a survival analysis showed that the probability for a chick to die from cat predation depended on the zone but not on cat abundance. Our monitoring also provided a fine‐scale investigation of the various sources of chick mortality. In addition to cat predation (24% of mortality overall), our data documented predation by giant petrels, for the first time in Kerguelen, and revealed a strong and unexpected effect of nest flooding on chick mortality. Overall, our results underline the need for future studies investigating interindividual variability in cat diet and spatial ecology.

Contrasting ecological contexts among treatment-seeking military sexual assault survivors: Consideration of relationships with sexual and gender minority identification.

Survivors of military sexual trauma (MST) seeking mental health services may present with concerns extending beyond symptom relief. Attention to social, economic, and coping resource contexts is salient for care consideration. Although those identifying as sexual and gender minorities (SGM) are overrepresented among service members exposed to assaultive MST, research contrasting ecological resource variability among treatment seekers is limited. The present study delineates modifiable risk and protective factors that might be used to inform MST-related health care for Veterans, broadly, and SGM-identifying Veterans, specifically. Veterans (N = 493, 12.8% identifying as SGM) presenting for treatment secondary to military sexual assault completed a semistructured clinical interview and intake survey including demographic characteristics, diversity-related factors, and access to psychosocial resources. SGM/non-SGM-identifying groups were contrasted on individual-, interpersonal-, and community-level ecological characteristics. SGM-identifying Veterans were less likely to report access to sufficient financial resources and had double the prevalence rate of housing instability in contrast to non-SGM-identifying Veterans. No significant differences emerged in terms of past-year interpersonal violence exposure, endorsement of helpful spiritual beliefs, or availability of social support based on SGM identification. Findings underscore the importance of attending to the intersection of SGM identity and ecological factors that can influence Veterans' clinical presentation and treatment engagement. Recommendations for provision of MST services are made. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Trophic niche partitioning among non-native fish species coexisting in a Colombian high Andean reservoir

The introduction of non-native species to a new environment poses a threat to local biological diversity, causing instability in the functioning of the ecosystem. The ecological effects caused by these species have been scarcely documented in the Magdalena basin. By studying predator–prey interactions, we characterized the trophic niche of three non-native species (Cyprinus carpio, Micropterus salmoides, and Oncorhynchus mykiss) that dominate a high Andean reservoir in the Magdalena basin. This study allows us to understand their specific feeding behaviors and how these behaviors facilitate their establishment in the reservoir. We evaluated the diversity of the prey they consume, their feeding strategy, and possible differences in the feeding scheme. Forty individuals were analyzed, with the highest representation of M. salmoides with 17 individuals, followed by C. carpio (13 individuals), and finally O. mykiss with 10 individuals. We identified twenty categories of food as prey for these species, with aquatic invertebrates and vegetation material being the predominant prey. The analysis of stomach contents in these samples suggested that they are representative for determining specialized or generalist feeding strategies. There were no differences in the number of prey items consumed by these three species. The analysis revealed that the feeding strategies are specific for each species. There was no overlap in the diet of C. carpio with the other two species, however, the composition of the diet is similar between M. salmoides and O. mykiss. Analyzing the diet of these non-native fish provides a useful tool for describing trophic interactions in this aquatic environment. Our results contribute information on the existing interactions amongst non-native species in the Magdalena basin, which is important for the development of strategies to manage and mitigate their impact.

The Agreement Under the U.N. Convention on the Law of the Sea on the Conservation & Sustainable Use of Marine Biological Diversity of Areas Beyond National Jurisdiction

On June 19, 2023, the Intergovernmental Conference on an international legally binding instrument under the United Nations Convention on the Law of the Sea on the conservation and sustainable use of marine biological diversity of areas beyond national jurisdiction (BBNJ Intergovernmental Conference) adopted, by consensus, the Agreement under the United Nations Convention on the Law of the Sea on the Conservation and Sustainable Use of Marine BioLogical Diversity of Areas Beyond National Jurisdiction (BBNJ Agreement or Agreement). The Agreement, which is grounded in and builds on the UN Convention on the Law of the Sea (UNCLOS), specifically addresses issues relating to the conservation and sustainable use of the marine biodiversity of the high seas. The adoption of the Agreement was the culmination of nearly two decades of work under the auspices of the UN General Assembly.

Grass Evolutionary Lineages Can Be Identified Using Hyperspectral Leaf Reflectance

AbstractHyperspectral remote sensing has the potential to map numerous attributes of the Earth’s surface, including spatial patterns of biological diversity. Grasslands are one of the largest biomes on Earth. Accurate mapping of grassland biodiversity relies on spectral discrimination of endmembers of species or plant functional types. We focused on spectral separation of grass lineages that dominate global grassy biomes: Andropogoneae (C4), Chloridoideae (C4), and Pooideae (C3). We examined leaf reflectance spectra (350–2,500 nm) from 43 grass species representing these grass lineages from four representative grassland sites in the Great Plains region of North America. We assessed the utility of leaf reflectance data for classification of grass species into three major lineages and by collection site. Classifications had very high accuracy (94%) that were robust to site differences in species and environment. We also show an information loss using multispectral sensors, that is, classification accuracy of grass lineages using spectral bands provided by current multispectral satellites is much lower (accuracy of 85.2% and 61.3% using Sentinel 2 and Landsat 8 bands, respectively). Our results suggest that hyperspectral data have an exciting potential for mapping grass functional types as informed by phylogeny. Leaf‐level hyperspectral separability of grass lineages is consistent with the potential increase in biodiversity and functional information content from the next generation of satellite‐based spectrometers.

Metagenomic exploration of Andaman region of the Indian Ocean

Ocean microbiome is crucial for global biogeochemical cycles and primary productivity. Despite numerous studies investigating the global ocean microbiomes, the microbiome composition of the Andaman region of the Indian Ocean remains largely unexplored. While this region harbors pristine biological diversity, the escalating anthropogenic activities along coastal habitats exert an influence on the microbial ecology and impact the aquatic ecosystems. We investigated the microbiome composition in the coastal waters of the Andaman Islands by 16S rRNA gene amplicon and metagenomic shotgun sequencing approaches and compared it with the Tara Oceans Consortium. In the coastal waters of the Andaman Islands, a significantly higher abundance and diversity of Synechococcus species was observed with a higher abundance of photosynthesis pigment-related genes to adapt to variable light conditions and nutrition. In contrast, Prochlorococcus species showed higher abundance in open ocean water samples of the Indian Ocean region, with a relatively limited functional diversity. A higher abundance of antibiotic-resistance genes was also noted in the coastal waters region. We also updated the ocean microbiome gene catalog with 93,172 unique genes from the Andaman coastal water microbiome. This study provides valuable insights into the Indian Ocean microbiome and supplements the global marine microbial ecosystem studies.

Challenging the existing order of knowledge sharing governance with digital sequence information on genetic resources

Abstract The Conference of the Parties to the Convention on Biological Diversity (CBD) have begun work on establishing a distinct multilateral mechanism to access and share the benefits (ABS) from digital sequence information on genetic resources (DSI), with the expectation of new monetary benefits. Regulating DSI for benefit sharing is problematic because efficiently and effectively regulating information requires defining DSI and identifying a suitable material proxy for the information because it is itself intangible (probably a levy or tax to access or use a database of information). More problematic is finding a compromise to the competing information governance frameworks: a dominant international intellectual property order that favours the free exchange of resources and information (generally favoured by the technology rich North); the taking of nature by States and re-ordering of biological resources to deliver benefits (generally favoured by the technology poor South); and, the claims of Indigenous Peoples according to their emerging rights in international law.

Easement of forests and forest land for the growing of perennial crops - the analysis of (non)functionality regarding the ecological network and ecosystem functions

By the Government's decisions from the period 2004 to 2013, a significant amount of state forests and forest land was eased for the growing of perennial crops (vineyards and olive yards) on a total surface of 24,310.24 ha throughout the state. Although greatly opposed by forestry professionals, since a vast amount of private agricultural land in the state is not utilized due to unresolved proprietary issues, these surfaces have become agricultural land by coming into force from the Forest Act of 2014. In case these dedicated surfaces are not put into function, the amount of non-utilized agricultural land is increasing and so is the danger from forest fires, and loss of biological and landscape diversity. On the other hand, if all these surfaces were utilized, a significant adverse impact on the ecological network would occur because on many ecological network sites more than 1% of the dedicated area consists of target habitat types (mostly maquis). The purpose of this paper is, to use GIS tools and analysis of the so-far signed easement contracts, to determine what amount of dedicated surfaces were utilized and point out the failure and environmental dangers that may arise from these decisions which can still be revoked, and the affected area can be brought back into the forest area of the Republic of Croatia, i. e. sustainable forest management can still be reinstated and forest functions restored.

THE ANATOMICAL STRUCTURE OF SYMPHYOTRICHUM SQUAMATUM, AN ALIEN PLANT IN ROMANIAN FLORA

In recent years, there has been a growing interest in alien plant species because of their substantial impact on biological diversity, ecosystems, and economy. These species are often characterized by their capacity to rapidly colonize diverse habitats. Symphyotrichum squamatum, an alien species for Romanian flora, displays invasive tendencies in various European countries, showing adaptability to a wide range of habitats, particularly those with high soil moisture. While previous research has focused on aspects of its invasiveness, limited information is available regarding the anatomical structure of its vegetative organs. Our study aims to identify the structural characteristics that may explain the species’ environmental preferences and adaptability to various habitat types. The results showed the presence of aeriferous tissues in the root and stem suggesting an affinity for humid conditions, while the lamina’s structure enhances light capturing efficiency and adaptability to salinity. These anatomical investigations provide valuable insights into understanding species’ environmental requirements and resilience, contributing to a better understanding of its adaptability to various habitats and potential impact on local ecosystems.

The geno-geo-climate nexus: contributions of geographic and ecological factors in shaping the genomic divergence of two closely related threatened rainforest species of Fontainea Heckel (Euphorbiaceae)

ContextProcesses that shape genomic and ecological divergence can reveal important evolutionary dynamics to inform the conservation of threatened species. Fontainea is a genus of rainforest shrubs and small trees including critically endangered and threatened species restricted to narrow, but complex geographic and ecological regions. Several species of Fontainea are subject to spatially explicit conditions and experience limited intra-specific gene flow, likely generating genetic differentiation and local adaptation.ObjectivesHere, we explored the genetic and ecological mechanisms underlying patterns of diversification in two, closely related threatened Fontainea species. Our aim was to compare spatial patterns of genetic variation between the vulnerable Fontainea australis (Southern Fontainea) and critically endangered F. oraria (Coastal Fontainea), endemic to the heterogeneous subtropical region of central, eastern Australia, where large-scale clearing has severely reduced rainforest habitat to a fraction (< 1%) of its pre-European settlement extent.MethodsWe used a set of 10,000 reduced-representation markers to infer genetic relationships and the drivers of spatial genetic variation across the two species. In addition, we employed a combination of univariate and multivariate genome-environment association analysis using a set of topo-climatic variables to explore potential patterns of local adaptation as a factor impacting genomic divergence.ResultsOur study revealed that Coastal Fontainea have a close genetic relationship with Southern Fontainea. We showed that isolation by distance has played a key role in their genetic variation, indicating that vicariance can explain the spatial genetic distribution of the two species. Genotype-environment analyses showed a strong association with temperature and topographic features, suggesting adaptation to localised thermal environments. We used a multivariate redundancy analysis to identify a range of putatively adapted loci associated with local environmental conditions.ConclusionsDivergent selection at the local-habitat scale as a result of dispersal limitations and environmental heterogeneity (including physical barriers) are likely contributors to adaptive divergence between the two Fontainea species. Our findings have presented evidence to indicate that Southern and Coastal Fontainea were comprised of distinct genetic groups and ecotypes, that together may form a single species continuum, with further phenotype research suggested to confirm the current species boundaries. Proactive conservation actions, including assisted migration to enhance the resilience of populations lacking stress-tolerant single nucleotide polymorphisms (SNPs) may be required to secure the long-term future of both taxa. This is especially vital for the critically endangered Coastal Fontainea given projections of habitat decline for the species under future climate scenarios.

Corroboration and contradictions in global biodiversity indicators

Biodiversity indicators measure progress toward global biodiversity goals, including the Kunming-Montreal Global Biodiversity Framework of the UN Convention on Biological Diversity. A suite of indicators is typically needed to capture the complexity of biodiversity. For the suite to be effective, it needs to capture the important aspects of the system, without over-representing some at the expense of others. It is therefore important to identify redundancies and contradictions between indicators within a suite. Comparing indicators derived from independent input data can also serve as a validation of indicator accuracy, or alternatively, identify unexpected behaviour and potential flaws or incompatibilities in data or construction. We examined the relationships between eight widely adopted global biodiversity indicators, all proposed for use in the monitoring framework of the Kunming-Montreal global biodiversity framework. We focused on the relationships between indicators driven by landuse data and those driven by species observations, testing for pairwise and multivariate correlations between indicator values for ecoregions. We used hierarchical clustering to identify spatial patterns in relationships between landuse and species response indicators. We found no strong correlation between landuse and species-response indicators in ecoregions with high landuse. Reasons for disagreement among indicators may include inaccurate, or coarse spatial data and mismatched baselines. We did find strong correlations between indicators sharing input data. We suggest that indicators selected for the Kunming-Montreal framework be systematically reviewed for correlations and recommend that suites of indicators should consider the independence of input data sources to minimise the risk of using correlated or biased measures of conservation progress.

Promotion of the conservation of biological diversity of interest for tourism in the coastal region of Ecuador

Promotion of the conservation of biological diversity of interest for tourism in the coastal region of Ecuador

Giving oranges and puppies: Children’s production of directional verbs in an emerging sign language from Oaxaca

Children’s acquisition of directional verbs in sign languages has received a lot of attention, but less is known about the sociocultural process of using these verbs, especially in the context of emerging sign languages in diverse language ecologies. Directional verbs are a common grammatical phenomenon of many sign languages in which some verbs such as ‘to give’ and ‘to take’ can move in the direction of one or more of its arguments for indicating grammatical relations between agents and patients or recipients. I discuss the case study of one signing family who uses ‘making hands’, an emic term for the signing practices of deaf Chatino people and their families in the San Juan Quiahije municipality in Oaxaca, Mexico. The family in question consists of a first-generation adult signer and two second-generation child signers, aged 4;6 and 5;3. This article describes their usage of directional verbs for making explicit requests, asking questions, and talking about hypothetical events, including presenting or withholding gifts as part of the cultural understandings and practices of the allocations of goods. Ethnographic data reveals that the children’s production of verbs may be facilitated by directed input in the form of directives from a deaf adult signer, extensive peer play, and visual access to signed adult interactions. This case study offers insight about input and socialization for the children’s usage of directional verbs in an emerging sign language in a local Mesoamerican ecology.