Amphibian Biodiversity Research Articles

Drivers of amphibian species richness in European ponds

Amphibians are commonly occurring inhabitants of most lentic freshwater ecosystems, yet their global populations are in alarming decline. Ponds in particular play a crucial role in supporting amphibian biodiversity. In this study, we identified the main drivers influencing amphibian species richness by conducting a comprehensive ecological characterization in 201 ponds across seven European countries spanning a large latitudinal and longitudinal gradient. The amphibian species richness in each of these ponds was assessed using environmental DNA metabarcoding on water samples. The relative influence of climatic, local abiotic and biotic, and land use variables on variation in species richness across ponds was quantified using boosted regression trees. Our results suggest that local factors, particularly chlorophyll‐a concentration, but also pond area and depth, are the main drivers of amphibian richness, together with climatic variables such as annual mean precipitation and temperature. The highest richness was observed in low‐nutrient, fishless, intermediate‐sized, shallow ponds, located in warmer regions with higher precipitation rates. These potential drivers of amphibian richness should be considered in the planning and implementation of amphibian conservation and management actions.

Climate change threatens amphibians and species representation within protected areas in tropical wetlands

Abstract Protected areas (PAs) are crucial for biodiversity conservation, yet climate change threatens their long‐term effectiveness by displacing species distributions. Among the most climate‐threatened organisms are the amphibians, highly dependent on water and wetlands. The world's largest continuous tropical wetland, the Pantanal and surroundings, is situated in the Upper Paraguay River Basin (UPRB) in South America. Of the 74 amphibian species found there, 4% are threatened. Less than 5% of the basin is within PAs. Recurrent droughts and increasing human pressures further endanger the region's semiaquatic fauna if adequate protection measures are not implemented. Using Ensemble of Small Models, we mapped habitat suitability of amphibian species and projected amphibian richness and composition across the UPRB. We applied null models to evaluate the representation of amphibian ranges within the current PA network under both current and future climate scenarios. To prioritize areas for PA network expansion under climate change scenarios, we used a systematic conservation planning algorithm. By 2100, over 80% of amphibian species from the study area are projected to lose suitable habitat, with 99% of amphibian assemblages facing climate‐driven species loss. Although the existing PA network had limited species representation, protecting on average less than 5% of amphibian ranges. However, 13.7% of PAs are expected to shelter more amphibian species than expected by chance under future climate conditions. Highlands, particularly in the northern and southeastern boundaries of the UPRB, are identified priority areas for PA network expansion due to their high projected changes in amphibian biodiversity. Synthesis and applications. Our findings reveal the extensive impacts of climate change on a major semiaquatic group in the world's largest tropical wetland. Although the current PA network in the Pantanal and surroundings safeguards fewer amphibians than expected, its limited coverage provides opportunities for systematic, data‐driven expansion to achieve the Post‐2020 Global Biodiversity Framework's 30 by 30 target. Expanding PAs is urgent, but addressing drivers of environmental degradation, including unsustainable practices in agriculture and livestock farming, is equally critical for conserving this biodiverse ecosystem.

Enhancing Farm Dams Increases Tadpole Abundance.

Understanding how agricultural and land management practices affect amphibian biodiversity is essential for conservation efforts in farmland. We investigated the impact of farm dam enhancement on tadpole abundance and growth in a highly modified farming landscape in south-eastern Australia. We completed detailed surveys on 52 farm dams (artificial ponds or agricultural reservoirs). These dams were categorized into two groups: enhanced (n = 28), which had undergone management activities such as fencing to prevent livestock access and facilitate revegetation, and control (n = 24), which had not received any intervention and were subject to standard management practices similar to adjacent paddocks. Our findings revealed a notable increase in tadpole abundance across all species in enhanced dams, with 92% of all observed tadpoles recorded in these dams. Factors such as higher dissolved oxygen and greater riparian vegetation cover were positively associated with tadpole abundance, while high pH levels showed a negative association. We found no evidence that tadpole growth was influenced by dam enhancement. Concerningly, when the invasive fish Gambusia holbrooki was present, tadpoles were smaller and at earlier developmental stages. Our findings highlight the potential benefits of strategic farm dam management for improving tadpole presence in agricultural landscapes.

Amphibian Diversity and Morphometric Analysis in the Kadayam Region of the Tirunelveli District, India

This study examines the morphometric and genetic diversity of frog species in the agro ecosystems of Kadayam region part of the Western Ghats, a biodiversity hotspot in India. By conducting an amphibian survey in Kadayam, Southern Western Ghats, eight species from three families were documented, with Dicroglossidae showing the highest species representation. Amphibians were sampled from various habitats-forests, water bodies, and cultivated lands between January and March 2017, with notable species Duttaphrynus melanostictus showing a high abundance in human occupied areas. Morphometric analyses were performed on features such as snout-vent length, head length, (SVL–SNOW-VENT LENGTH; HL - HEAD LENGTH; HW-HEAD WIDTH; LHU–LENGTH OF THE HUMERUS; FOL-FOREARM LENGTH; THL-THIGH LENGTH; TL-TIBIA LENGTH; IOD-INTERORBITAL DIAMETER; IND-INTER NOSTRIL DISTANCE; ED-EYE DIAMETER; END–EYE–NOSTRIL DISTANCE; and FL -FOOT LENGTH) and limb proportions across species, correlating these to understand habitat adaptations. Results indicated high endemism and diversity within frog populations of the Western Ghats, highlighting conservation needs due to threats like habitat modification and pollution. The high positive correlations among primary body dimensions (such as SVL, head length, and total length) suggest that these species exhibit proportional growth in these features, a common trait in morphometric analyses. This study underscores the significance of morphometric approaches in amphibian biodiversity assessments in ecologically sensitive regions.

Reintroduction of resistant frogs facilitates landscape-scale recovery in the presence of a lethal fungal disease

Vast alteration of the biosphere by humans is causing a sixth mass extinction, driven in part by an increase in infectious diseases. The emergence of the lethal fungal pathogen Batrachochytrium dendrobatidis (Bd) has devastated global amphibian biodiversity. Given the lack of any broadly applicable methods to reverse these impacts, the future of many amphibians appears grim. The Sierra Nevada yellow-legged frog (Rana sierrae) is highly susceptible to Bd infection and most R. sierrae populations are extirpated following disease outbreaks. However, some populations persist and eventually recover, and frogs in these recovering populations have increased resistance against infection. Here, we conduct a 15-year reintroduction study and show that frogs collected from recovering populations and reintroduced to vacant habitats can reestablish populations despite the presence of Bd. In addition, the likelihood of establishment is influenced by site, cohort, and frog attributes. Results from viability modeling suggest that many reintroduced populations have a low probability of extinction over 50 years. These results provide a rare example of how reintroduction of resistant individuals can allow the landscape-scale recovery of disease-impacted species, and have broad implications for amphibians and other taxa that are threatened with extinction by novel pathogens.

Effects of Breeding Habitat on the Abundance and Body Condition of Two <i>Pelophylax</i> Species in Agriculture and Urban Landscapes

Amphibians play a key role in structuring biological assemblages of agricultural landscapes, but they are threatened globally by agricultural intensification. Numerous studies have considered the morphology of animals to be an indicator of the health of a population, but data about the differences in amphibian morphology in different breeding habitats in agricultural landscapes are still missing. Here, we investigated the differences in the abundance and morphology of Pelophylax plancyi and Pelophylax nigromaculatus in the three waterbody types: fields, forests, and parks in agricultural landscapes in Nanjing, China. Our results emphasize that both frog species in forests and parks had significantly higher body condition indexes than those in fields, whereas their abundance in fields habitat was higher than those of the two other habitat types. Our results demonstrated the importance of non-agricultural habitats (such as forests and parks) in agricultural landscapes to the persistence of both Pelophylax species. We recommend preserving field ecosystems by constructing diverse waterbodies other than fields in rural areas and maintaining microhabitats in parks and forests to improve amphibian biodiversity in Nanjing.

Detection of Amphibian Species based on Cytochrome b Metabarcoding using High-Throughput Sequencing Approach

Abstract Amphibians are essential in terrestrial and freshwater ecosystems, contributing to multiple ecological functions. The presence of amphibians in a particular area usually signifies a healthy ecosystem as it indicates that the habitat meets the requirements of their life cycles. However, the amphibian population have been declining due to human activities including pollution and habitat destruction. Researchers are using new methods to monitor amphibian diversity to address this decline. Conventional sampling methods were compared with new DNA metabarcoding techniques using advanced sequencing platforms. Therefore, this study aims to analyze the early detection of amphibian species using Cytochrome b (Cytb) gene with metabarcoding methods and next generation sequencing techniques. Thirty-one samples were used in this study and underwent processes such as extraction, amplification using Cytb gene, library preparation, and sequencing. The result showed that the total number of reads was 658,655, with reads in OTUs numbered 474,061. Out of 31 samples, only three species were successfully detected: Fejervarya limnocharis with the highest abundance (20.6%), followed by Polypedates leucomystax and Pulchrana baramica. The results indicate that Cytb has low capabilities in detecting amphibian species, which might be due to its highly conserved region and the lack of databases for this gene. For future studies, an enhanced DNA metabarcoding procedure should be applied, including better choice of genetic marker and bioinformatics pipelines to minimize error and to improve the accuracy and reliability of detecting amphibian’s species using NGS and DNA metabarcoding. This would enhance efforts to protect amphibian biodiversity.

No sex-dependent mortality in an amphibian upon infection with the chytrid fungus, Batrachochytrium dendrobatidis.

One of the major factors driving the currently ongoing biodiversity crisis is the anthropogenic spread of infectious diseases. Diseases can have conspicuous consequences, such as mass mortality events, but may also exert covert but similarly severe effects, such as sex ratio distortion via sex-biased mortality. Chytridiomycosis, caused by the fungal pathogen Batrachochytrium dendrobatidis (Bd) is among the most important threats to amphibian biodiversity. Yet, whether Bd infection can skew sex ratios in amphibians is currently unknown, although such a hidden effect may cause the already dwindling amphibian populations to collapse. To investigate this possibility, we collected common toad (Bufo bufo) tadpoles from a natural habitat in Hungary and continuously treated them until metamorphosis with sterile Bd culture medium (control), or a liquid culture of a Hungarian or a Spanish Bd isolate. Bd prevalence was high in animals that died during the experiment but was almost zero in individuals that survived until the end of the experiment. Both Bd treatments significantly reduced survival after metamorphosis, but we did not observe sex-dependent mortality in either treatment. However, a small number of genotypically female individuals developed male phenotype (testes) in the Spanish Bd isolate treatment. Therefore, future research is needed to ascertain if larval Bd infection can affect sex ratio in common toads through female-to-male sex reversal.

Long-term hypothermic storage of oocytes of the European common frog Rana temporaria at various pressure regimes in gas mixtures based on oxygen, carbon monoxide, and nitrous oxide

In recent years, the challenge of preserving amphibian biodiversity has increasingly been addressed through technologies for the short-term storage of unfertilized spawn at low positive temperatures. Previously the possibility of using a 6.5 atm gaseous mixture of carbon monoxide and oxygen for prolonged hypothermic preservation of unfertilized oocytes for more than 4 days was shown. This study aimed to investigate the viability of oocytes R. temporaria preserved under conditions of hypothermia at 2.5, 3 and 6.5 excess atm pressure in the various gas mixture compositions (CO, N2O, O2) and pure oxygen. The use of pressure up to 3 excess atmospheres was significantly beneficial compared to 6.5 atm at the 7 days storage period. The results indicate that oxygen pressure is a critical factor in maintaining oocyte viability. Admixing CO or N2O to oxygen reduced variability in the results but did not significantly affect the measured indicators (fertilization, hatching) in the experimental groups. The composition CO + O2 (0.5/3.5 ratio, 3 excess atm) reliably extended the shelf life of viable oocytes, indistinguishable from native controls by fertilization and hatching rates, to 4 days. After 7 days, oocytes exhibited fertilization and hatching rates that were 79 % and 48 % compared to native control. Reducing the pressure of the preserving gas mixture to 3 atm, as utilized in this study, simplifies the practical implementation of gas preservation technology for maintaining endangered amphibian species during breeding in laboratory conditions.

Environmental factors and host sex influence the skin microbiota structure of Hong Kong newt (Paramesotriton hongkongensis) in a coldspot of chytridiomycosis in subtropical East Asia.

Chytridiomycosis, an infectious skin disease caused by the chytrid fungi, Batrachochytrium dendrobatidis and B. salamandrivorans, poses a significant threat to amphibian biodiversity worldwide. Antifungal bacteria found on the skin of chytrid-resistant amphibians could potentially provide defense against chytridiomycosis and lower mortality rates among resistant individuals. The Hong Kong newt (Paramesotriton hongkongensis) is native to East Asia, a region suspected to be the origin of chytrids, and has exhibited asymptomatic infection, suggesting a long-term coexistence with the chytrids. Therefore, the skin microbiota of this resistant species warrant investigation, along with other factors that can affect the microbiota. Among the 149 newts sampled in their natural habitats in Hong Kong, China, putative antifungal bacteria were found in all individuals. There were 314 amplicon sequence variants distributed over 25 genera of putative antifungal bacteria; abundant ones included Acinetobacter, Flavobacterium, and Novosphingobium spp. The skin microbiota compositions were strongly influenced by the inter-site geographical distances. Despite inter-site differences, we identified some core skin microbes across sites that could be vital to P. hongkongensis. The dominant cores included the family Comamonadaceae, family Chitinophagaceae, and class Betaproteobacteria. Moreover, habitat elevation and host sex also exhibited significant effects on skin microbiota compositions. The antifungal bacteria found on these newts offer an important resource for conservation against chytridiomycosis, such as developing probiotic treatments for susceptible species.

ВПЛИВ ПОГОДНИХ УМОВ НА ЧИСЕЛЬНІСТЬ АМФІБІЙ ТА РЕПТИЛІЙ В УМОВАХ ПРИРОДНОГО ЗАПОВІДНИКА «МЕДОБОРИ» ЗА ПЕРІОД 2016–2020 РОКІВ

Based on the results of long-term observations, the influence of weather conditions on the dynamics of the number and biodiversity of amphibians and reptiles over five years in the "Medobory" nature reserve was analyzed. The number, reproduction, metamorphosis stages, and distribution of amphibians and reptiles depend significantly on climatic conditions. Low temperatures in spring inhibit spawning and larval development. During critical periods, a low amount of precipitation (e.g., 15.7 mm in May 2016, 9.1 mm in April 2018, and 12.1 mm in April 2019, compared to the norm of 45 mm) also negatively affects amphibian populations. This factor led to the disappearance of Triturus cristatus Laur., Lissotriton vulgaris L. (an outbreak was noted in 2014), and Bufo viridis Laur. from the reserve (last observed at MG-1 in 2013), as well as the less common Hyla orientalis Bedriaga. Amphibians and reptiles were recorded during the active period along two regular herpetological routes, MG-1 and MG-2, and observations were made during field research, covering all areas suitable for the reproduction and habitat of amphibians. Eleven species of amphibians have been confirmed in the reserve, belonging to six families: Salamandridae, Bombinatoridae, Pelobatidae, Bufonidae, Hylidae, and Ranidae. Among these amphibians, five species are listed in Appendix II of the Berne Convention, and one species (T. cristatus Laur.) is included in the Red Book of Ukraine. The most common amphibian species are Rana temporaria L., Rana arvalis Nilsson, and Bufo bufo L. Frogs such as Pelophylax lessonae Camerano and Pelophylax ridibundus Pallas occasionally occur within the reserve, as they require an aquatic environment even after reproduction. There are seven species of reptiles in the reserve and one in the immediate vicinity, out of the ten recorded in the Ternopil region. These reptiles occupy the most suitable habitats for their survival. The species represent five families: Emydidae (freshwater turtles), Anguidae (spindly lizards), Lacertidae (true lizards), Colubridae (snakes), and Viperidae (vipers). Common species include Lacerta agilis Andrz., Natrix natrix L., and Anguis fragilis L. Six species of reptiles are included in Appendix II of the Berne Convention, and the common copperhead (Coronella austriaca Laur.) is included in the Red Book of Ukraine.

Hidden hotspots of amphibian biodiversity in China

Identifying and protecting hotspots of endemism and species richness is crucial for mitigating the global biodiversity crisis. However, our understanding of spatial diversity patterns is far from complete, which severely limits our ability to conserve biodiversity hotspots. Here, we report a comprehensive analysis of amphibian species diversity in China, one of the most species-rich countries on Earth. Our study combines 20 y of field surveys with new molecular analyses of 521 described species and also identifies 100 potential cryptic species. We identify 10 hotspots of amphibian diversity in China, each with exceptional species richness and endemism and with exceptional phylogenetic diversity and phylogenetic endemism (based on a new time-calibrated, species-level phylogeny for Chinese amphibians). These 10 hotspots encompass 59.6% of China's described amphibian species, 49.0% of cryptic species, and 55.6% of species endemic to China. Only four of these 10 hotspots correspond to previously recognized biodiversity hotspots. The six new hotspots include the Nanling Mountains and other mountain ranges in South China. Among the 186 species in the six new hotspots, only 9.7% are well covered by protected areas and most (88.2%) are exposed to high human impacts. Five of the six new hotspots are under very high human pressure and are in urgent need of protection. We also find that patterns of richness in cryptic species are significantly related to those in described species but are not identical.

Loss of amphibian species alters periphyton communities in montane ponds

Amphibian larvae can affect the structure and functioning of freshwater ecosystems, but their effects have been little explored although amphibian biodiversity is rapidly declining. Given that larvae of different amphibian species belong to different trophic levels, their effects on freshwater communities and processes can be expected to differ, with herbivores likely having direct effects on algae and predators having indirect effects through trophic cascades. We explored this question through a mesocosm experiment conducted in montane ponds, using an anuran and a urodele species affected by emergent diseases. We used different scenarios of reduction and loss of one or both species, and compared them to a control scenario representing a typical amphibian community in the study area composed of four species, with total larval density held constant. Loss of the anuran resulted in lower chlorophyll concentration and algal density, likely due to replacement by more efficient grazers. Loss of the urodele produced similar trends but weaker, possibly due to an increase of invertebrate grazing activity in the absence of this predator. Our study shows how the loss of amphibian species can alter the structure of montane ponds, but also how the mechanisms involved and the intensity of effects differ for different species.

Batrachochytrium salamandrivorans is necronotic: carcasses could play a role in Bsal transmission

IntroductionBatrachochytrium salamandrivorans (Bsal) poses a major threat to global amphibian biodiversity. It is essential we understand Bsal transmission to develop better-informed management strategies. Infected carcasses are an important source of transmission for several human and wildlife disease systems; however, they have not been examined as sources for Bsal exposure. Here, we evaluated whether infected newt carcasses could contribute to Bsal transmission dynamics.MethodsWe cohoused infected carcasses with susceptible newts in two cohousing chamber types (partitioned or non-partitioned) at three timepoints post-mortem ([0,24[, [24,48, [48,72] hrs). The partitioned chamber prevented newt-to-newt contact hence only allowed indirect, waterborne transmission of zoospores. We measured shedding rates of infected carcasses at each post-mortem timepoint and monitored infection status and mortality of susceptible newts which were exposed during cohousing events.ResultsOur results indicate carcasses are capable of transmitting Bsal to susceptible newts up to at least 72 hrs post-mortem, even without live newts directly contacting carcasses. All susceptible newts in each chamber type and post-mortem period became infected and >90% experienced disease-induced mortality. Bsal genomic copies/uL in skin swabs taken from infected carcasses were high, averaging 7.4x105, 8.6x105, and 2.0x106 at 24, 48, and 72 hrs post-mortem, respectively. Water samples collected from cohousing chambers averaged 2743 Bsal genomic copies/uL (approximately 1357 zoospores) and did not decline over 72 hrs. DiscussionOur results indicate Bsal infection can occur rapidly between infected carcasses and susceptible aquatic salamanders via indirect and direct transmission pathways, and carcasses may prolong outbreaks by increasing the duration that infected individuals remain infectious. Carcass removal may be a strategy to reduce Bsal transmission and the impacts of outbreaks.

A Dataset of Amphibian Species in U.S. National Parks

National parks and other protected areas are important for preserving landscapes and biodiversity worldwide. An essential component of the mission of the United States (U.S.) National Park Service (NPS) requires understanding and maintaining accurate inventories of species on protected lands. We describe a new, national-scale synthesis of amphibian species occurrence in the NPS system. Many park units have a list of amphibian species observed within their borders compiled from various sources and available publicly through the NPSpecies platform. However, many of the observations in NPSpecies remain unverified and the lists are often outdated. We updated the amphibian dataset for each park unit by collating old and new park-level records and had them verified by regional experts. The new dataset contains occurrence records for 292 of the 424 NPS units and includes updated taxonomy, international and state conservation rankings, hyperlinks to a supporting reference for each record, specific notes, and related fields which can be used to better understand and manage amphibian biodiversity within a single park or group of parks.

Diversity, ecology and conservation of anurans from Ramnagar, West Bengal, India

This study aimed to assess and understand the amphibian biodiversity and conservation status in the Ramnagar area, West Bengal, India. Amphibians play crucial roles in ecosystem functioning and serve as indicators of environmental health. However, their populations worldwide are facing significant threats, highlighting the need for comprehensive assessments and conservation strategies. In this study, extensive field surveys were conducted in the Ramnagar area to document amphibian species richness, evenness, diversity and asses the influence of environmental factors (vegetation cover, pH, dissolved oxygen in water, humidity, and temperature) on species diversity. A combination of visual encounter surveys and passive sampling techniques was employed to ensure a comprehensive coverage of the study area. The results of the study revealed a moderate diversity with a total of 10 species and 4 families identified. Duttaphrynus melanostictus was the most abundant, whereas Fajervarya orissaensis was the least observed. Shannon and Simpson indexes suggest a moderately diverse community of anurans, Margalef’s Index indicates a more even distribution of individuals across species, suggesting a balanced community, while the Shannon Equitability Index suggests that the amphibian community is moderately diverse, with a reasonable variety of species present. Of the tested ecological factors, dissolved oxygen and humidity have the most significant effect on species diversity.

Integrated phylogenetic analyses reveal the evolutionary, biogeographic, and diversification history of Asian warty treefrog genus Theloderma (Anura, Rhacophoridae).

Asian warty treefrogs, genus Theloderma, are morphologically variable arboreal frogs endemic to Southeast Asia and Southern China. However, integrated systematic studies are lacking, and knowledge of the genus in terms of diversity, origin, and historical diversification remains limited. To address these knowledge gaps, we used three mitochondrial and five nuclear gene fragments to reconstruct the Theloderma phylogeny, estimate divergence times, and examine the biogeography of the genus. Phylogenetic and species delimitation analyses suggest that the genus Theloderma comprises three major clades corresponding to two subgenera and seven species groups, and mPTP identified at least 12 putative cryptic species, suggesting that species diversity has been underestimated. Biogeographic analyses indicated that most recent common ancestor of Theloderma originated in the Indochina Peninsula during the Middle Oligocene (ca. 27.77 Ma) and the splitting of Clade A to C occurred in the Late Oligocene (ca. 23.55-25.57 Ma). Current biogeographic patterns result from two distinct processes: insitu diversification in the Indochina Peninsula and dispersal in multiple areas, namely southward dispersal to the Malay Peninsula and Borneo, northeastward dispersal to Southern China, northward dispersal to the Himalayas, and dispersal from Southern China to the Indochina Peninsula. Ancestral character reconstruction suggests that the ancestor of Theloderma may have possessed a small body size, rough dorsal skin, and absence of vomerine teeth and hand webbing, and that these four characters have undergone multiple evolutions. Principal component analysis based on eight bioclimatic variables did not clearly distinguish the three major clades of Theloderma, suggesting that species in these clades may occupy similar climatic ecological niches. Our research highlights the importance of orogeny and paleoclimatic changes, in shaping amphibian biodiversity in mountain ecosystems.

HERPETOFAUNA OF THE “EL MINERAL DE NUESTRA SEÑORA DE LA CANDELARIA” RESERVE: A BIOLOGICAL TREASURE IN SINALOA, MEXICO

Mexico hosts an exceptional diversity of approximately 1 421 species of amphibians and reptiles, many of which are endemic to the country. However, the Sinaloa region has remained largely unexplored due to access challenges and social conflicts. This study focuses on analyzing the biodiversity of amphibians and reptiles in the “El Mineral de Nuestra Señora de la Candelaria” reserve in Sinaloa, Mexico. This reserve, located in the Sierra Madre Occidental, covers 1 256 hectares of diverse habitats, including tropical deciduous and sub-deciduous forests. Through fieldwork conducted between 2017 and 2020, 55 species of amphibians and reptiles were documented in the reserve, of which 34 are endemic to Mexico, highlighting its importance as a refuge for native biodiversity. Furthermore, 20 species are protected by Mexican regulations and are listed on the IUCN Red List, indicating their threatened status. This reserve significantly contributes to Sinaloa’s herpetofaunal landscape, safeguarding approximately 32.4% of its diversity and over 53% of the genera present in the state. The presence of endemic and endangered species underscores the need for specific conservation measures. In conclusion, this listing provides a detailed insight into the biological richness of amphibians and reptiles in the “El Mineral de Nuestra Señora de la Candelaria” reserve, emphasizing its value as a refuge for unique and threatened species. The results urge the implementation of effective conservation strategies to protect these unique ecosystems in the context of global environmental changes.

Natural area visitors’ willingness to pay for amphibian conservation in a global biodiversity hotspot

Transfer of harmful pathogens from imported pet animals to natural populations of wildlife is a new challenge in protected area management. Understanding visitors’ preferences for, and value they place on, protecting biodiversity from such disturbances is important in developing effective management interventions. By conducting a visitor survey at two natural areas in the Southern Appalachian region of the United States, a global hotspot of amphibian biodiversity, this study examined natural area visitors’ willingness to pay (WTP) to conserve wild amphibian populations under two different assumptions for the distribution of WTP – normal and log-transformed. Results from the retained model assuming a normal distribution for WTP showed that visitors were willing to pay US$10.55 (95% CI US$9.61—US$11.50) per visit in donation to ensure the persistence of healthy amphibian populations. Determinants of individual WTP under both model specifications included the perceived threat to amphibians from pathogen transmission in natural areas they visit, their self-assessed familiarity with general knowledge about amphibians, the level of importance they placed on the benefits to humans from amphibian biodiversity, and annual household income. These findings are useful in understanding the economic value of amphibian diversity to natural area visitors, and in assessing the viability of a visitor-supported mechanism to fund projects mitigating anthropogenic threats to amphibian biodiversity.

One Health Approach to Globalizing, Accelerating, and Focusing Amphibian and Reptile Disease Research-Reflections and Opinions from the First Global Amphibian and Reptile Disease Conference.

The world's reptiles and amphibians are experiencing dramatic and ongoing losses in biodiversity, changes that can have substantial effects on ecosystems and human health. In 2022, the first Global Amphibian and Reptile Disease Conference was held, using One Health as a guiding principle. The conference showcased knowledge on numerous reptile and amphibian pathogens from several standpoints, including epidemiology, host immune defenses, wild population effects, and mitigation. The conference also provided field experts the opportunity to discuss and identify the most urgent herpetofaunal disease research directions necessary to address current and future threats to reptile and amphibian biodiversity.