Biodiversity Loss Research Articles

Climate Resilience in Farm Animals: Transcriptomics-Based Alterations in Differentially Expressed Genes and Stress Pathways

The livestock sector, essential for maintaining food supply and security, encounters numerous obstacles as a result of climate change. Rising global populations exacerbate competition for natural resources, affecting feed quality and availability, heightening livestock disease risks, increasing heat stress, and contributing to biodiversity loss. Although various management and dietary interventions exist to alleviate these impacts, they often offer only short-lived solutions. We must take a more comprehensive approach to understanding how animals adapt to and endure their environments. One such approach is quantifying transcriptomes under different environments, which can uncover underlying pathways essential for livestock adaptation. This review explores the progress and techniques in studies that apply gene expression analysis to livestock production systems, focusing on their adaptation to climate change. We also attempt to identify various biomarkers and transcriptomic differences between species and pure/crossbred animals. Looking ahead, integrating emerging technologies such as spatialomics could further accelerate genetic improvements, enabling more thermoresilient and productive livestock in response to future climate fluctuations. Ultimately, insights from these studies will help optimize livestock production systems by identifying thermoresilient/desired animals for use in precise breeding programs to counter climate change.

How can biodiversity strategy and action plans incorporate genetic diversity and align with global commitments?

Abstract National, subnational, and supranational entities are creating biodiversity strategy and action plans (BSAPs) to develop concrete commitments and actions to curb biodiversity loss, meet international obligations, and achieve a society in harmony with nature. In light of policymakers’ increasing recognition of genetic diversity in species and ecosystem adaptation and resilience, this article provides an overview of how BSAPs can incorporate species’ genetic diversity. We focus on three areas: setting targets; committing to actions, policies, and programs; and monitoring and reporting. Drawing from 21 recent BSAPs, we provide examples of policies, knowledge, projects, capacity building, and more. We aim to enable and inspire specific and ambitious BSAPs and have put forward 10 key suggestions mapped to the policy cycle. Together, scientists and policymakers can translate high level commitments, such as the Convention on Biological Diversity’s Kunming–Montreal Global Biodiversity Framework, into concrete nationally relevant targets, actions and policies, and monitoring and reporting mechanisms.

Impacts of climate change and deforestation on fire risk in the Amazon basin

Fires in the Amazon rainforest are a result of anthropogenic activity in the region. As the Amazon rainforest becomes vulnerable to fire through deforestation and climate change, it is imperative to understand how the fire risk will change in future years—not only for the potential of the forest to become a source of carbon to the atmosphere if burned but also for the potential loss of unique biodiversity and its critical role in regional and global climate. Therefore, we used the Eta regional climate model driven by 3 global climate models to simulate 2 distinct scenarios: one with a high CO2 emission (RCP8.5-DEF15) path and another with a high CO2 emission path and the total deforestation of the Amazon rainforest (RCP8.5-DEFTOT). Combining the multi-model ensemble of temperature and precipitation, we calculated the Keetch-Byram Drought Index (KBDI) and analyzed the results for the dry and wet seasons. In both scenarios, precipitation is reduced and maximum temperature increased. The greatest changes occur during the dry season and with the RCP8.5-DEFTOT scenario. In RCP8.5-DEF15, the KBDI is greatly affected, showing positive anomalies in both seasons. With the addition of deforestation, the fire risk is further exacerbated, thus highlighting the role of the forest in suppressing optimal conditions for fire. Additionally, more than half of the basin area is predicted to experience a high risk of fire in extreme years. Our results emphasize the importance of reducing global emissions and deforestation of the Amazon, given that fire activity could threaten the future of the Amazon rainforest.

The chromosome-level genome assembly and annotation of an invasive forest pest Obolodiplosis robiniae.

Biological invasion is a major global problem, leading to the loss of biodiversity and species extinction, and causing huge economic losses to countries. Obolodiplosis robiniae is a major invasive forest pest that has caused economic losses in Asia and Europe. Here, the chromosome- level genome of O. robiniae was assembled using the PacBio platform and Hi-C technology. A contig-level genome with a length of 199.49 Mb and a contig N50 of 4.66 Mb was assembled. Approximately 98.05% of contigs were successfully anchored to four chromosomes using Hi-C assisted genome assembly. The genome integrity was assessed to be 90.3% based on BUSCOs analysis. The high-quality genome provides valuable data for the study of invasive species, and a foundation for the understanding the biology and ecology of O. robiniae.

Best practices for the naming of species

Abstract The process of describing a species has evolved considerably since the modern age of taxonomy was initiated with the publication of the 10th edition of Linnaeus’ Systema Naturæ in 1758. Nevertheless, the basic unit of the taxonomic framework remains the species group (species and subspecies). As a result, it is incumbent upon individuals undertaking species-group descriptions to strive for the highest standards, just as it is for reviewers and editors to hold authors to those same standards. The clear and mounting biodiversity crisis, the increasing proliferation of journals, the multiplication of journals publishing only in electronic format, the decline in the number of trained taxonomists, and the complex and at times misinterpreted wording of the rules governing taxonomy have resulted in a growing number of species names that are unavailable because one or more aspects required of a species-level taxonomic description are lacking. Here, we present best practices for the required elements of a taxonomic description and identify some of the common pitfalls leading to an inadequate description resulting in an unavailable name. We also suggest best practices that would enhance taxonomic descriptions and make them more informative.

Effect of organic farming on root microbiota, seed production and pathogen resistance in winter wheat fields

Societal Impact StatementAgricultural intensification is a major driver of biodiversity decline in agrosystems. For instance, it has been shown that conventional farming leads to a decline in soil microbial diversity and triggers a strong selection process, altering the functioning of the whole ecosystem. The present study shows that organic farming increases diversity and affects composition of crop plant microbiota, mostly as a response to field management and soil characteristics. Furthermore, crop plant microbiota influences crop production and resistance to pathogens. Therefore, agricultural practices affect plant performance through microorganism‐mediated changes, which may be important pillars of future sustainable crop production.Summary Agricultural intensification threatens biodiversity, but the effects of intensification on microorganisms are still overlooked despite their role in ecosystem functioning. Microorganisms associated with plants provide many services that affect plant growth and health. Organic farming is expected to strongly affect species composition, richness, and their interactions. We analyzed the effect of the farming system on endophytic microbial assemblages associated with winter wheat plants and plant performance in the field. We collected environmental data through farmer interviews, soil analyses, and plant inventories and analyzed root microbiota at vegetative and flowering stages. Organic farming increased fungal and bacterial diversity associated to wheat plants and affected species composition in most phyla. This effect was mostly due to soil characteristics and field management and a little to plant diversity in the field. Microbial responses were more pronounced at the late developmental stage, likely as a result of accumulative effect of management actions during plant development. Seed production and resistance to pathogens were related to specific phyla that are important for seed production and/or wheat resistance to septoriose. This work advances our understanding of how agricultural practices affect plant performance through microorganism‐mediated changes and supports the use of microorganisms as pillars of sustainable crop production.

Spatial Distribution of Burned Areas from 1986 to 2023 Using Cloud Computing: A Case Study in Amazonas (Peru)

Wildfire represents a significant threat to ecosystems and communities in the Department of Amazonas, Peru, causing losses in biodiversity and land degradation and affecting socioeconomic security. The objective of this study was to analyze the spatial and temporal distribution of burned areas (BAs) from 1986 to 2023 to identify recurrence patterns and their impact on different types of land use and land cover (LULC). Landsat 5, 7, and 8 satellite images, processed by Google Earth Engine (GEE) using a decision tree approach, were used to map and quantify the affected areas. The results showed that the BAs were mainly concentrated in the provinces of Utcubamba, Luya, and Rodríguez de Mendoza, with a total of 1208.85 km2 burned in 38 years. The most affected land covers were pasture/grassland (38.25%), natural cover (forest, dry forest, and shrubland) (29.55%) and agricultural areas (14.74%). Fires were most frequent between June and November, with the highest peaks in September and August. This study provides crucial evidence for the implementation of sustainable management strategies, fire prevention, and restoration of degraded areas, contributing to the protection and resilience of Amazonian ecosystems against future wildfire threats.

Re-imagining the use of integrated assessment models from a social science perspective - lessons from the sustainable development pathways (SDP)

Abstract Socio-political factors in Integrated Assessment Models (IAMs), and their scenario narratives often lack transparency for policymakers and interdisciplinary scholars. As these tools increasingly support sustainable development goals, their assumptions and methodologies require scrutiny, particularly from social scientists. We address critiques of climate isolationism, overemphasis on technological transitions, and insufficient inter- and transdisciplinarity, advocating for robust interdisciplinary integration and clearer methodological transparency. Our recommendations stem from expert interviews and over 200 stakeholders across 30 countries from 2019 to 2024, emphasizing the need for cohesive theory and comprehensive social science engagement to refine these critical tools.
Our main case study uses a new scenario set, the Sustainable Development Pathways (SDPs), that made substantial efforts to address social sciences critiques. The SDPs consist of both narratives and IAM-quantified target-seeking scenarios that are supported by social science concepts and theories to ensure not only theoretical coherence, but also their credibility among policymakers. As such tools are increasingly used to facilitate policies and actions for sustainability transformation, questions are raised about how they can effectively represent the complexities behind the current polycrisis that is marked by the climate crisis, biodiversity loss, economic inequality and social injustice. 
The paper concludes by reflecting on the remaining challenges and open questions related to the role of exogenous sociopolitical factors, the potential for scenarios to transcend political ideologies, and the need for ongoing adaptation of SDPs to reflect the dynamic global context. It calls for continued engagement and exploration of these issues to ensure the scientific representation of sustainable and equitable futures.

How to make land use policy decisions: Integrating science and economics to deliver connected climate, biodiversity, and food objectives

Land use change is crucial to addressing the existential threats of climate change and biodiversity loss while enhancing food security [M. Zurek et al., Science 376, 1416-1421 (2022)]. The interconnected and spatially varying nature of the impacts of land use change means that these challenges must be addressed simultaneously [H.-O. Pörtner et al., Science 380, eabl4881 (2023)]. However, governments commonly focus on single issues, incentivizing land use change via "Flat-Rate" subsidies offering constant per hectare payments, uptake of which is determined by the economic circumstances of landowners rather than the integrated environmental outcomes that will be delivered [G. Q. Bull et al., Forest Policy Econ. 9, 13-31 (2006)]. Here, we compare Flat-Rate subsidies to two alternatives: "Land Use Scenario" allocation of subsidies through consultation across stakeholders and interested parties; and a "Natural Capital" approach which targets subsidies according to expected ecosystem service response. This comparison is achieved by developing a comprehensive decision support system, integrating new and existing natural, physical, and economic science models to quantify environmental, agricultural, and economic outcomes. Applying this system to the United Kingdom's net zero commitment to increase carbon storage via afforestation, we show that the three approaches result in significantly different outcomes in terms of where planting occurs, their environmental consequences, and economic costs and benefits. The Flat-Rate approach actually increases net carbon emissions while Land Use Scenario allocation yields poor economic outcomes. The Natural Capital targeted approach outperforms both alternatives, providing the highest possible social values while satisfying net zero commitments.

Curbing the crises, gauging the gap: measuring the nexus between gender and the environment

Abstract Women and men interact with the environment differently. Although data on the gender and environment nexus is only recently starting to become more widely available, existing evidence shows that, in many countries, women and men’s livelihoods, roles at home, and decision-making actions are widely different, and thus so are their contributions to environmental conservation and degradation. Simiarly, climate change, pollution and biodiversity loss affect women and men in different ways, but the limited availability of related data still prevents a full understanding of the multiplicity of ways in which these differences manifest. In recent years, numerous methodological advancements have taken place, which are enabling the production of gender and environment statistics to better understand these connections. From dedicated surveys to uses of non-conventional data sources, empirical evidence on this topic is increasingly available. Still, substantial efforts are needed to fill existing data gaps and gain a thorough understanding of the connections. This paper showcases available evidence on various aspects of the gender-environment nexus, and highlights possible solutions to fill remaining data gaps.

Analysis of the Availability and Utilization of Green Open Space (GOS) in Kahayan Hilir Subdistrict, Pulang Pisau Regency Using the DPSIR Approach

This study aims to analyze the availability and utilization of Green Open Space (GOS) in Kahayan Hilir Subdistrict, Pulang Pisau Regency, utilizing the DPSIR (Driving Forces, Pressures, States, Impacts, Responses) approach and the Indonesian Green Blue Index (IHBI) model. This research addresses the growing demand for adequate GOS due to rapid urbanization and economic development. The study identifies the driving forces behind GOS changes, pressures arising from land conversion and population growth, the current state of GOS, and the public's perception of GOS utilization. Data collection involved interviews with stakeholders, systematic field observations, and a comprehensive analysis of regional planning documents. The results indicate that the availability of GOS in Kahayan Hilir is still below government standards, resulting in environmental issues such as declining air quality, biodiversity loss, and increased urban heat island effects. Public perception indicates an awareness of the importance of GOS for health and recreation, although accessibility and facilities remain limited. This study recommends increasing both the quantity and quality of GOS through stricter policies, community participation, and public-private partnerships to support environmental quality and the well-being of residents in Kahayan Hilir Subdistrict.

Building a Circular Economy for Lithium: Addressing Global Challenges

AbstractAs countries worldwide race toward a green transition, the demand for electric vehicles is surging, and with it comes a growing need for batteries. However, the push for increased domestic mining to secure these materials raises significant concerns about environmental sustainability. Even with stringent regulations, the environmental impact of mining can be profound, posing risks such as biodiversity loss, water pollution, and broader ecological damage. Furthermore, geopolitical tensions could arise as countries whose economic interests are threatened by these initiatives may react adversely. Local communities might also resist mining projects due to concerns over environmental degradation, health risks, and disruptions to their livelihoods. Given the critical importance of metals in the ecological transition, this challenge must be approached with the same urgency and global coordination as a pandemic response. Just as the world mobilized unprecedented resources to tackle COVID‐19, a similarly robust approach is necessary to ensure the availability of critical metals for a sustainable future. This paper suggests potential pathways for academic, technological, and societal advancements within the framework of a circular economy for lithium, aiming to secure a sustainable supply of this essential resource.

Essential steps for establishing a large‐scale passive acoustic monitoring for an elusive forest bird species: the Eurasian Woodcock (Scolopax rusticola)

In light of global biodiversity loss, comprehensive monitoring of species and biodiversity is fundamental for effective conservation management. Technology‐driven approaches, particularly in the field of bioacoustics, have gained importance, with autonomous recording units (ARUs) such as the AudioMoth being a notable example. However, a systematic application of this technology in large‐scale passive acoustic monitoring (PAM) schemes is challenging because of a lack of species‐specific calibration techniques and information on survey design. Using Eurasian Woodcock Scolopax rusticola as our focal species, we present application‐oriented guidelines for testing and implementing a terrestrial, single‐species PAM scheme. In this process, we assessed the species‐specific detection space of AudioMoths, considering the influence of recording setup and location. ARUs had similar detection rates as a human observer when set up in locations with low vegetation. A further comparative analysis of bioacoustic and traditional roding surveys confirmed the comparability of the two methods. The last step comprised an evaluation of BirdNET as a means of automated species detection. Although BirdNET reliably detected Woodcock occurrence, our results underline the need for rigorous testing of computer‐aided data analysis as well as recording hardware before establishing PAM. We demonstrate that low‐cost open source ARUs and open access analysis tools can yield results on a par with traditional survey methods, and we provide specific recommendations for PAM for Eurasian Woodcock.

Scaling models for Regreening Africa: enhancing agroecological integration through smallholders’ assets and agency in Kenya

Urgent action is needed to address climate change, land degradation, and biodiversity loss. The Regreening Africa project (2017–2023), recently recognized as a UN World Restoration Flagship, aimed to reverse land degradation over large areas of land for the triple benefit of people, biodiversity, and climate in eight African countries. Based on projections and early lessons learned, the project sought to identify sustainable scaling models to achieve its ambitious targets. The so-called “Asset-Based Community-driven Development (ABCD) in Regreening” project aimed to demonstrate the positive contribution of deliberate community engagement and co-design. The project introduced ABCD sessions to 30 purposively selected community groups in the Regreening intensification sites in western Kenya. ABCD combines a unique set of framings, methods, and processes that focus on people’s assets and agency, and emphasizes the importance of their attitudes toward self and others for sustainable behavior change. To evidence that ABCD intrinsically contributes to sustainable adoption and scaling of Regreening practices, the project developed the F-ACT+ tool to assess the alignment between ABCD and agroecological practices, and collected baseline and endline data from 300 project and 300 non-project participants. Results showed accelerated agroecological integration among ABCD project participants. ABCD participants showed significant improvements in nine agroecological principles and eight system components, particularly in the economic diversification, social values and diets, and knowledge co-creation principles, as well as in the pest and disease, household, and value chain system components. Summary ATT between ABCD and non-ABCD respondents was positive and significant in 10 principles and eight system components. The results support the synergistic contribution of ABCD to projects targeting sustainable behavior change at the individual and collective levels. Due to its focus on outcomes, this study provided limited insight into the specific mechanisms of ABCD, which are the subject of a separate publication on parallel theory-based contribution analysis work.

Environmental health and human health-unifying strategies, conceptual framework and green prescriptions: a review

The intricate relationship between environmental conditions and human health is increasingly acknowledged as a critical area of public health research. As global challenges such as climate change, urbanization, and biodiversity loss become more pronounced, understanding the connections between these environmental factors and health outcomes is essential for developing effective public health strategies. This analysis delves into the complex interactions that exist between environmental influences and health, emphasizing the urgent need for integrated approaches that simultaneously promote ecological sustainability and enhance community well-being. Environmental degradation has significant implications for human health, with many deaths linked to preventable environmental health risks. These risks encompass a wide range of issues, including air and water pollution, exposure to hazardous substances, and the impacts of climate change, all of which disproportionately affect vulnerable populations. A conceptual framework is proposed to elucidate the multifaceted connections between environmental exposures, health determinants, and health outcomes, enabling a clearer understanding of how these elements interrelate. Cohesive strategies, such as the one health approach, advocate for collaboration across various sectors, including public health, veterinary health, and environmental management, to address the shared challenges of health and environmental degradation. Additionally, the principles of planetary health highlight the interdependence of ecosystems and human well-being, underscoring the need for sustainable management of natural resources to safeguard health outcomes. This article also explores the concept of "green prescriptions," which involve recommending nature-based interventions to enhance physical, mental, and social well-being. These prescriptions have demonstrated effectiveness in promoting health while fostering a sense of environmental stewardship. By synthesizing current evidence and successful case studies, this review emphasizes the significance of adopting a holistic perspective that integrates environmental considerations into healthcare practices. Ultimately, fostering collaboration among various stakeholders will contribute to the development of resilient communities that are better equipped to navigate the intertwined challenges of health and environmental sustainability.

Knowledge co‐production praxis in sustainability science: Insights from three contexts

AbstractKnowledge co‐production is needed as never before to support social change in the face of climate, water, biodiversity, and other sustainability crises. Co‐production brings together diverse groups and their ways of knowing to generate new knowledges and practices that reconfigure or generate transformative social changes and that invite reflexivity. Within sustainability sciences, tensions exist between descriptive, analytical framings of co‐production used to interrogate knowledge‐power relations and instrumental or normative framings used to build such relations. The former has been criticised for being overly descriptive and difficult to translate into policy outcomes and the latter for failing to sufficiently interrogate power dynamics and for perpetuating existing inequities. As researchers, how are we to navigate this tension? Co‐production praxis involves reconfiguring knowledge‐power relations for just and transformative social changes. I suggest what is needed is a critical lens on those relations to underpin and guide feasible and action‐oriented processes and outcomes for such changes. In three ways, I present and reflect on co‐production contexts with different temporal, spatial and epistemological characteristics. These contexts are analysing historical co‐production of knowledge of coastal freshwater floodplain Country of the Northern Territory, facilitating the Kunwinjku Seasons calendar and enabling reflexive co‐production praxis with sustainability science researchers at a national science institution. I demonstrate the need within each context to weave analytical, practical, and reflexive work to reconfigure fairer societal outcomes and to pay greater attention to socio‐institutional changes arising from our engaged work.

Predicting habitat functionality using habitat network models in urban planning

Recently, the European Union adopted a Nature restoration law that aims to stop the ongoing decline of biodiversity and even bring back nature to cities. This is challenging as urbanization is an ongoing process that thrives for more land and densification. In this paper we describe a new Open-Source GIS-tool that automates habitat network analyses and simultaneously generates several maps that can be used for assessments, targeting species survival in urban environments. One result of particular interest is the habitat functionality map that combines values of habitat quality and connectivity. We tested the tool’s ability to predict habitat functionality using amphibian occurrence data observed from the city of Gothenburg in Sweden. Our evaluation shows that habitat functionality was generally a good predictor of amphibian distribution. However, the predictability was sensitive to the cartographic representativity of the input biotope map used. Also, predictions of habitat functionality improved when estimating dispersal probabilities using the Cost-distance algorithm, compared to when using Euclidean distance from reproductive habitats. This finding supports the need to use connectivity models that are responsive to variation and changes in roads, traffic volumes and buildings when performing effect analyses on biodiversity in cities. Finally, we demonstrate how the tool can be used to easily identify areas where restoration measures can effectively increase habitat functionality for target species. This can help planners to find efficient solutions for increasing biodiversity within urban areas.

Messy natures: The political aesthetics of nature recovery

Abstract Current aspirations for nature recovery to address biodiversity loss and climate change will involve changing what landscapes look like, which can create challenging aesthetic experiences for members of the public. In this study, we interrogate the role of aesthetics in shaping visions of nature recovery, focussing on the construction and contestation of ‘mess’ and ‘messiness’ in nature recovery. We examine the political aesthetics of messy natures using a qualitative approach investigating visual and text materials across different sites of production in Britain, from newspapers to social media, paying particular attention to visual materials Our results show how the mediated debate around ‘rewilding’ urban environments and a beaver‐themed garden winning a horticultural show are examples of a backlash against untidy environments through a politicised discourse, linking messy nature with left‐wing ideologies, in contrast to the perceived orderliness and conservative ideals of good citizenship associated with tidiness. In contrast, social media imagery of messy and wild gardens demonstrates how mess is consciously framed aesthetically as a desirable ‘look’ for restored nature, symbolising progressive and environmentalist ideals of good citizenship through activities such as nature‐friendly gardening and initiatives such as ‘No Mow May’, which celebrate the aesthetic of a more biodiverse messiness. And finally, we traced how environmental advocacy organisations are prefiguring the future of nature recovery in Britain through commissioned graphical representations that portray a ‘tamed messiness’ and the return of charismatic megafauna. Although these aesthetics may challenge prevailing pastoral aesthetics, they normalise continued inequality in the form of concentrated land ownership, especially in the Scottish Highlands. Policy implications. Our analysis shows that aesthetics play an important role in shaping views of what is ‘natural’, ‘normal’ and ‘good’ and therefore desirable in what a future (recovered) nature looks like. We suggest that nature recovery initiatives take aesthetics seriously, by interrogating how idealisations of ‘how nature should look’ may influence decision‐making alongside ecological and other considerations. Taking aesthetics seriously also opens avenues for engaging with social and environmental justice, where alternative aesthetics can help in imagining more socially and environmentally just futures. Read the free Plain Language Summary for this article on the Journal blog.

Parasites in a Changing World: Troublesome or in Trouble?

There are plenty of reasons to believe that parasite populations will respond to biodiversity loss, warming, pollution, and other forms of global change. But will global change enhance transmission, increasing the incidence of troublesome parasites that put people, livestock, and wildlife at risk? Or will parasite populations decline in abundance-or even become extinct-suggesting trouble on the horizon for parasite biodiversity? Here, I explain why answers have thus far eluded us and suggest new lines of research that would advance the field. Data collected to date suggest that parasites can respond to global change with increases or decreases in abundance, depending on the driver and the parasite. The future will certainly bring outbreaks of some parasites, and these should be addressed to protect human and ecosystem health. But troublesome parasites should not consume all of our research effort, because this changing world contains many parasite species that are in trouble.

Overview of plant species translocations in Luxembourg

In Luxembourg, 34 % of native plants and archaeophytes are considered threatened or regionally extinct. Species translocations have been implemented in Luxembourg for more than 25 years as a conservation strategy to counteract biodiversity loss. In this overview, we have compiled data on conservation actions implemented between 1997 and 2023. The information collected includes the translocation locations, the plant taxa targeted, the methods used, and the monitoring conducted. For a subset of translocations, we have also included data on the survival of planted juveniles. Examples of translocations from various habitat types were also presented to illustrate different approaches. We found that 585 translocations involving 99 taxa from 26 families were initiated by four main institutions in Luxembourg. The conservation actions mainly targeted Endangered and Critically Endangered, but also Least Concern species. Translocations mainly took place in open land habitats, particularly in grasslands and arable land, with a higher density in the southwestern part of the country. 39 % of the translocations took place in national nature reserves whereas 21 % were in areas without any legal protection status. Planting young plants was the preferred method rather than sowing seeds. In 89 % of the translocations, monitoring was conducted by recording survival of the plants, while natural recruitment of new individuals was rarely recorded. 68 % of the translocations were judged successful by the practitioners, mostly based on transplant survival. Detailed data for the subset of 31 grassland species showed that the survival rate of about half of the species considered successful varied between 25 and 71 %. Translocations are essential for the long-term conservation of plant species, but protection and adaptive management of translocation sites are also essential. Translocations conform to national and European priorities for increasing habitat restorations to counteract species decline. To improve conservation outcomes, actions such as translocations need to be consistently documented, monitoring standardized, and experience needs to be shared among stakeholders.