Biodiesel is a promising alternative solution for the energy crisis, as it is renewable, biodegradable, non - toxic and it has a fully competitive edge with petrol diesel in most technical aspects. Because of the increasing global request for diesel fuel consumption, consumption of non-renewable fossil fuels, exhaust emissions, all these led to search about alternative fuels. Biodiesel is one of the potential alternatives for depleting fossil fuels. Biodiesel fuel is an eco-friendly and renewable source of energy. Biodiesel fuel is an eco-friendly and renewable source of energy. Biodiesel is one of the best solutions and alternatives available to all countries of the world. Inedible plants and vegetable oils such as Jatropha oil, Karanji Pongamia oil, Neem oil, Jojoba oil, Cottonseed oil, Linseed oil, Mahua oil, Deccan hemp oil, Kusum oil, Orange oil, and Rubber seed oil were used as biodiesel in diesel engine. Biodiesel is produced by chemical methods from vegetable oils, but the use of vegetable oils is a high cost.Scientists searched for other sources and at the same time has a low cost. They found that waste cooking oil is the ideal solution to that problem. Four methods to minimize the high viscosity of vegetable oils to enable their use in common diesel engines without operational problems such as engine charges have been investigated: blending with petro diesel, pyrolysis, microemulsification (solvent blending), and transesterification. Transesterification is by far the most common method. Only the transesterification reaction leads to the products commonly known as biodiesel, i.e., alkyl esters of oils and fats. Biodiesel was produced from waste Cooking-oil by transesterification process have been applied for most countries to improve and raise the national energy return. Blends of WCOB and diesel were used instead of pure diesel fuel in diesel engine. However, the spray characteristic of the injected fuel depends on different parameters such as fuel injection pressure, the temperature of the injected fuel, ambient pressure, fuel viscosity, and fuel density.In internal combustion engines, such as the diesel engine used to conduct the experiment, a 20 mm window and a transparent glass box are used to facilitate the visualization of spray characteristics. The spray characteristics of the diesel/ waste cooking oil biodiesel blends fuel were studied experimentally at different injection pressure and temperature. Spray characteristics of biodiesel WCOB / diesel under various injection pressures of 150 to 300 bar and injection temperature of 25 to 275 oC have been measured and illustrated by sparing the fuel blends in a surrounding area using a high-speed Digital camera. In this work, the biodiesel/diesel blend of B40D60 has been tested. The experimental data shows the WCOB biodiesel of B40D60 gives longer spray tip penetration and spray angle are smaller than those of diesel fuels at different pressure and temperature.