Biodegradation Studies Research Articles

Effect on thermal, mechanical, and biodegradable properties of plasma‐treated fish scale powder/linear low‐density polyethylene polymer composite films

AbstractIn the present work, we report the effect of low‐temperature plasma treatment on thermal, mechanical, and biodegradable properties of polymer composite blown films prepared from carp fish scale powder (CFSP) and linear low‐density polyethylene (LLDPE). The CFSP was melt compounded with LLDPE using a filament extruder to prepare 1, 2, and 3 wt.% of CFSP in LLDPE polymer composite filaments. These filaments were further pelletized and extruded into blown films. The blown films extruded with 1, 2, and 3 wt.% of CFSP in LLDPE were tested for thermal and mechanical properties. It was observed that the tensile strength decreased with the increased loading content of CFSP, and 1% CFSP/LLDPE exhibited the highest tensile strength. To study the effect of low‐temperature plasma treatment, 1% CFSP/LLDP polymer composite with high tensile strength was plasma treated with O2 and SF6 gas before blow film extrusion. The 1% CFSP/LLDPE/SF6‐extruded blown films showed increased thermal decomposition, crystallinity, tensile strength, and modulus. This may be due to the effect of crosslinking by the plasma treatment. The maximum thermal decomposition rate, crystallinity %, tensile strength, and modulus obtained for 1% CFSP/LLDPE/SF6 film were 500.02°C, 35.79, 6.32 MPa, and 0.023 GPa, respectively. Furthermore, the biodegradability study on CFSP/LLDPE films buried in natural soil for 90 days was analyzed using x‐ray fluorescence. The study showed an increase in phosphorus and calcium mass percent in the soil. This is due to the decomposition of the hydroxyapatite present in the CFSP/LLDPE biocomposite.

Biodegradation of polypropylene by filter-feeding marine scallop Mizuhopecten yessoensis: infrared spectroscopy evidence

The problem of environmental pollution by plastics is global in nature and needs to be addressed as soon as possible. Realization of the importance of this problem contributed to the study of degradation and biodegradation of synthetic polymers. It turned out that the driving force of plastic fragmentation along with abiotic factors can be biotic. Based on the above, we investigated the in vitro biodegradation of polypropylene (PP) fragments in digestive gland homogenates and crystalline styles of the bivalve mollusk Mizuhopecten yessoensis. Fourier transform infrared spectroscopy showed changes in the chemical composition of functional groups on the plastic surface. Enzyme complexes of crystalline styles enhanced the biodegradation of PP fragments to a larger extent than did digestive glands. The results obtained using M. yessoensis as an example suggest that marine phytophagous filter-feeding invertebrates may accelerate the biodegradation of synthetic polymers. The study provides a basis for rethinking the nature of relationships between marine invertebrates and microplastic polluting the marine environment.

Biodegradability Study of Modified Chitosan Films with Cinnamic Acid and Ellagic Acid in Soil.

Currently, natural polymer materials with bactericidal properties are extremely popular. Unfortunately, although the biopolymer material itself is biodegradable, its enrichment with bactericidal compounds may affect the efficiency of biodegradation by natural soil microflora. Therefore, the primary objective of this study was to evaluate the utility of fungi belonging to the genus Trichoderma in facilitating the degradation of chitosan film modified with cinnamic acid and ellagic acid in the soil environment. Only two strains (T.07 and T.14) used chitosan films as a source of carbon and nitrogen. However, their respiratory activity decreased with the addition of tested phenolic acids, especially cinnamic acid. Addition of Trichoderma isolates to the soil increased oxygen consumption during the biodegradation process compared with native microorganisms, especially after application of the T.07 and T.14 consortium. Isolates T.07 and T.14 showed high lipolytic (55.78 U/h and 62.21 U/h) and chitinase (43.03 U/h and 41.27 U/h) activities. Chitinase activity after incorporation of the materials into the soil was higher for samples enriched with T.07, T.14 and the consortium. The isolates were classified as Trichoderma sp. and Trichoderma koningii. Considering the outcomes derived from our findings, it is our contention that the application of Trichoderma isolates holds promise for expediting the degradation process of chitosan materials containing bactericidal compounds.

Synergistic anticancer effect of Pistacia lentiscus essential oils and 5-Fluorouracil co-loaded onto biodegradable nanofibers against melanoma and breast cancer

Chemoresistance and severe toxicities represent major drawbacks of chemotherapy. Natural extracts, including the essential oils of Pistacia lentiscus (PLEO), exhibit substantial anticancer and anti-inflammatory activities where different cancers are reported to dramatically recess following targeting with PLEO. PLEO has promising antimicrobial, anticancer, and anti-inflammatory properties. However, the therapeutic properties of PLEO are restricted by limited stability, bioavailability, and targeting ability. PLEO nanoformulation can maximize their physicochemical and therapeutic properties, overcoming their shortcomings. Hence, PLEO was extracted and its chemical composition was determined by GC–MS. PLEO and 5-Fluorouracil (5FU) were electrospun into poly-ε-caprolactone nanofibers (PCL-NFs), of 290.71 nm to 680.95 nm diameter, to investigate their anticancer and potential synergistic activities against triple-negative breast cancer cells (MDA-MB-231), human adenocarcinoma breast cancer cells (MCF-7), and human skin melanoma cell line (A375). The prepared nanofibers (NFs) showed enhanced thermal stability and remarkable physical integrity and tensile strength. Biodegradability studies showed prolonged stability over 42 days, supporting the NFs use as a localized therapy of breast tissues (postmastectomy) or melanoma. Release studies revealed sustainable release behaviors over 168 h, with higher released amounts of 5FU and PLEO at pH 5.4, indicating higher targeting abilities towards cancer tissues. NFs loaded with PLEO showed strong antioxidant properties. Finally, NFs loaded with either PLEO or 5FU depicted greater anticancer activities compared to free compounds. The highest anticancer activities were observed with NFs co-loaded with PLEO and 5FU. The developed 5FU-PLEO-PCL-NFs hold potential as a local treatment of breast cancer tissues (post-mastectomy) and melanoma to minimize their possible recurrence.Graphical

Chlorpyrifos Biodegradation Study in Egyptian Agricultural Soil

Chlorpyrifos Biodegradation Study in Egyptian Agricultural Soil

A Comparative Biodegradation Study to Assess the Ultimate Fate of Novel Highly Functionalized Hydrofluoroether Alcohols in Wastewater Treatment Plant Microcosms and Surface Waters.

Per- and polyfluoroalkyl substances (PFAS) are a class of chemicals present in a wide range of commercial and consumer products due to their water-repellency, nonstick, or surfactant properties, resulting from their chemical and thermal stability. This stability, however, often leads to persistence in the environment when they are inevitability released. We utilized microbial microcosms from wastewater treatment plant (WWTP) sludge to determine how employing different functional groups such as heteroatom linkages, varying chain lengths, and hydrofluoroethers (HFEs) will impact the ultimate fate of these novel PFAS structures. A suite of five novel fluorosurfactant building blocks (F7C3OCHFCF2SCH2CH2OH (FESOH), F3COCHFCF2SCH2CH2OH (MeFESOH), F7C3OCHFCF2OCH2CH2OH (ProFdiEOH), F7C3OCHFCF2CH2OH (ProFEOH), and F3COCHFCF2OCH2CH2OH (MeFdiEOH)) and their select transformation products, were incubated in WWTP aerobic microcosms to determine structure-activity relationships. The HFE alcohol congeners with a thioether (FESOH and MeFESOH) were observed to transform faster than the ether congeners, while also producing second-generation HFE acid products (F7C3OCHFC(O)OH (2H-3:2 polyfluoroalkyl ether carboxylic acid [PFECA]) and F3COCHFC(O)OH (2H-1:2 PFECA). Subsequent biodegradation experiments with 2H-1:2 PFESA and 2H-1:2 PFECA displayed no further transformation over 74 days. Surface water Photofate experiments compared 2H-1:2 PFECA, and 2H-1:2 polyfluorinated ether sulfonate (PFESA) with their fully fluorinated ether acid counterparts, and demonstrated the potential for both HFE acid species to completely mineralize over extended periods of time, a fate that highlights the value of studying novel PFAS functionalization. Environ Toxicol Chem 2024;43:2297-2305. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Microwave‐assisted synthesis, characterization, and application of tragacanth gum grafted copolymer as bioflocculant for the treatment of textile wastewater using coagulation‐flocculation technique: Swelling behavior and biodegradation studies

AbstractThis study presents, the synthesis of microwave‐assisted biodegradable polyacrylamide grafted tragacanth gum (Tr‐g‐PAM), which was utilized as a bioflocculant to treat textile effluent and compared against the conventional flocculant. The reaction parameters for maximal grafting were optimized by varying the reaction factors. The grafting of acrylamide onto the backbone of Tragacanth gum was confirmed by FTIR, XRD, TGA, and SEM–EDX studies. Swelling studies were performed in deionized water and different salts. Tr‐g‐PAM exhibited pH and temperature‐dependent swelling behavior. Biodegradation studies of the graft copolymer were carried out by the soil composting method. Tr‐g‐PAM showed 91.54% degradation within 60 days, compared to Tragacanth, which degraded within 15 days. SEM techniques were used to describe various phases of biodegradation. Flocculation performances of the graft copolymers were evaluated in simulated colloidal wastewater. Influencing variables such as bioflocculant dosage, pH, and flocculation mixing speed were examined. The maximum decolorization at the optimum pH of 6 and 120 mg/L dosage was found to be 91.8% and 88.5% for Tr‐g‐PAM and Tragacanth. In conclusion, a biodegradable Tr‐g‐PAM with better flocculation efficiency than that of Tragacanth and other commercial flocculants would be a promising choice as an eco‐friendly adsorbent for the removal of contaminants from water resources.

Enhanced Biodegradation Rate of Poly(butylene adipate-co-terephthalate) Composites Using Reed Fiber.

To enhance the degradability of poly(butylene adipate-co-terephthalate) (PBAT), reed fiber (RF) was blended with PBAT to create composite materials. In this study, a fifteen day degradation experiment was conducted using four different enzyme solutions containing lipase, cellulase, Proteinase K, and esterase, respectively. The degradation process of the sample films was analyzed using an analytical balance, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). The PBAT/RF composites exhibited an increased surface hydrophilicity, which enhanced their degradation capacity. Among all the enzymes tested, lipase had the most significant impact on the degradation rate. The weight loss of PBAT and PBAT/RF, caused by lipase, was approximately 5.63% and 8.17%, respectively. DSC analysis revealed an increase in the melting temperature and crystallinity over time, especially in the film containing reed fibers. FTIR results indicated a significant weakening of the ester bond peak in the samples. Moreover, this article describes a biodegradation study conducted for three months under controlled composting conditions of PBAT and PBAT/RF samples. The results showed that PBAT/RF degraded more easily in compost as compared to PBAT. The lag phase of PBAT/RF was observed to decrease by 23.8%, while the biodegradation rate exhibited an increase of 11.8% over a period of 91 days. SEM analysis demonstrated the formation of more cracks and pores on the surface of PBAT/RF composites during the degradation process. This leads to an increased contact area between the composites and microorganisms, thereby accelerating the degradation of PBAT/RF. This research is significant for preparing highly degradable PBAT composites and improving the application prospects of biodegradable green materials. PBAT/RF composites are devoted to replacing petroleum-based polymer materials with sustainable, natural materials in advanced applications such as constructional design, biomedical application, and eco-environmental packaging.

Experimental study of biodegradability of organic waste with industrial waste combined with effluents: a comparison by vermicomposting technology

Experimental study of biodegradability of organic waste with industrial waste combined with effluents: a comparison by vermicomposting technology

Overview of Soil Xenobiotics and their Biological Remediation Strategies

Xenobiotic pollution of the environment is a global phenomenon brought on by human activity as a result of increased urbanisation and population expansion. Pesticides, petrol, solvents, alkanes, polycyclic hydrocarbons (PAHs), antibiotics, artificial azo dyes, pollutants (polychlorinated biphenyls and dioxins), and polyaromatic, chlorinated, and nitroaromatic substances are examples of xenobiotics. Xenobiotics are primarily defined as substances that are foreign to a living thing and have an inclination to collect in the environment,where they can be harmful for existing ecosystem. The natural environment is impacted in a number of ways, both short- and long-term, when these dangerous contaminants are released. The scientific study of xenobiotic compound biodegradation—which uses microorganisms' catabolic powers to break down poisonous and hazardous xenobiotics—has received a lot of attention in the last few decades. Numerous bacteria possessing remarkable capacity for biodegrading xenobiotic substances have been identified, and their novel degradation routes have been clarified. The field of molecular approach to biodegradation research is fairly young. Therefore, in order to create efficient and environmentally beneficial "green" technology, it is imperative to investigate the microbial biodiversity.

Comparative e-waste plastics biodegradation efficacy of monoculture Pseudomonas aeruginosa strain PE10 and bacterial consortium under in situ condition.

A significant amount of electronic obsoletes or electronic waste (e-waste) is being generated globally each year; of these, ~20% of obsolete electronic items have plastic components. Current remediation practices for e-waste have several setbacks due to its negative impact on the environment, agro-ecosystem, and human health. Therefore, comparative biodegradation studies of e-waste plastics by monoculture Pseudomonas aeruginosa strain PE10 and bacterial consortium consisting of Achromobacter insolitus strain PE2 (MF943156), Acinetobacter nosocomialis strain PE5 (MF943157), Pseudomonas lalkuanensis PE8 (CP043311), and Stenotrophomonas pavanii strain PE15 (MF943160) were carried out in situ. Biological treatment of e-waste with these candidates in soil ecosystems has been analyzed through diversified analytical techniques such as Fourier transform infrared spectroscopy (FTIR), thermogravimetric-derivative thermogravimetry-differential thermal analysis (TG-DTG-DTA), and scanning electron microscopy (SEM). Both P. aeruginosa strain PE10 and the bacterial consortium have a tremendous ability to accelerate the biodegradation process in the natural environment. However, FTIR analysis implied that the monoculture had better efficacy than the consortium, and it was consistent until the incubation period used for the study. Some polymeric bonds such as ν C=C and δ C-H were completely removed, and ν C=C ring stretching, νasym C-O-C, νsym C-H, etc. were introduced by strain PE10. Furthermore, thermal analysis results validated the structural deterioration of e-waste as the treated samples showed nearly two-fold weight loss (WL; 6.8%) than the untreated control (3.1%) at comparatively lower temperatures. SEM images provided the details of surface disintegrations. Conclusively, individual monoculture P. aeruginosa strain PE10 could be explored for e-waste bio-recycling in agricultural soil ecosystems thereby reducing the cost, time, and management of bioformulation in addition to hazardous pollutant reduction.

Strategies for biofilm optimization of plastic-degrading microorganisms and isolating biofilm formers from plastic-contaminated environments

Abstract The perpetual disposal of plastic waste, combined with ineffective waste management strategies, has resulted in widespread environmental plastic pollution. Microbial plastic biodegradation represents an emerging solution to this problem. However, biodegradation studies tend to overlook the fundamental prerequisite of initial surface colonization via biofilm formation. This study had two independent but connected aims relating to plastic surface colonization by microorganisms: to enhance biofilm formation by known plastic degraders, with translational potential for improved plastic degradation, and to isolate microorganisms from microplastic contaminated environments with the ability to colonize plastic surfaces. Planktonic and biofilm responses to diverse carbon and energy sources were investigated over 7 days, using Bacillus subtilis 168, Fusarium solani (Martius) Saccardo, Ideonella sakaiensis 201-F6, Pseudomonas putida KT2440, and Rhodococcus ruber C208. This enabled optimal conditions for biofilm formation by each strain to be determined. In parallel, environmental samples containing synthetic or natural polymeric substances (anaerobic digestate, landfill leachate, and microplastic contaminated compost) were incubated with polyethylene and polyethylene terephthalate films, to isolate microorganisms capable of colonizing their surfaces. This yielded eight bacterial isolates from three genera: Bacillus, Lysinibacillus, and Proteus. These genera contain species that have been shown to degrade plastics and other recalcitrant synthetic polymers, demonstrating the success of our approach. This study also suggests that discrete plastic types may create different ecological niches which can be exploited by unique bacterial colonizers. Our findings underscore the importance of considering plastic colonization by microbial biofilms in the context of their biodegradation.

Characterization of Bioplastics from Breadfruit (Artocarpus altilis) Starch and Carboxy Methyl Cellulose (CMC) with Glycerol Plasticizer

Research on the synthesis of bioplastic from breadfruit (Artocarpus altilis) starch and carboxy methyl cellulose (CMC) has been successfully carried out with the addition of glycerol. Samples are conducted by varying the glycerol concentrations (10%, 20%, 30%, and 40%) at 60oC, respectively. The bioplastic product was tested using tensile strength, elongation, water content absorption, biodegradability, and Fourier transform-infra infrared (FT-IR). The tensile strength properties from bioplastic result evaluated is 34.26 N/mm2. The elongation at break of bioplastic is found at 21.4%. Water content results from bioplastic of 44%. Biodegradability study that bioplastics of 20% in 32 days. Correspondingly, the FT-IR confirmed the functional groups of bioplastics from breadfruit starch and CMC with glycerol.

Integrated approach to the study of biodegradation of composite materials for the restoration of hard dental tissues

Objective. To study the stress-strain state of demineralized tooth enamel after its impregnation with a low-viscosity composite in a long-term period of functioning in the oral cavity in a series of equivalent cyclic tests; to study the correlation of the experimental results with clinical observations of the treatment of enamel caries using a modified infiltration technique. Materials and methods. The parameters of laser holographic interferometry and atomic force microscopy, reflecting the timing, type and severity of deformation defects in samples of demineralized tooth enamel were analyzed in a series of equivalent cyclic tests. During the experiment the samples underwent classical infiltration with composite using ICON technology (main group) and a 2-stage enamel caries treatment technique, including carrying out a time-modified conditioning of classical infiltration and the final stage (stages) of enamel coating with a bioactive hybrid glass ionomer (comparison group). Results. The first signs of the deformation defects generation in demineralized enamel filtered on the classical method (the appearance of perifocal microcracks) were recorded in 11.2 % of the tested samples after 0.9 · 106 cycles (equivalent to 9 months of functioning of a tooth with treated enamel caries in the oral cavity), during further cyclic tests the percentage of samples with local defects increased progressively. Signs of a violation of the stress-strain state in the enamel after combined treatment (samples of the comparison group) were revealed in cycles corresponding to 2–3 years of being in an artificial environment of the oral cavity. Experimental data correlated with the clinical observations both in time and frequency of complications (secondary/recurrent caries) in the long term after treatment of enamel caries by various methods. Conclusions. In the process of equivalent cyclic tests, heterogeneous elastic-plastic deformations develop in the thickness of a section of demineralized enamel impregnated with a flowing composite and along its perifocal zones, most pronounced at the interface between intact and treated enamel, which leads to the development of local defects, delaminations, cracks, and destruction of the structurally heterogeneous environment of tooth enamel. According to the results of the comparative analysis, the most favorable results in terms of the timing of development, frequency and severity of deformation defects were obtained in samples of demineralized enamel after its treatment using a modified caries infiltration technique followed by coating with a bioactive hybrid glass ionomer.

Impact of Polylactic Acid Fibers in Cellulose Nonwoven Mulch Blends on Biodegradability and Performance-An Open Field Study.

The performance and degradation of nonwoven mulches made from viscose, jute, hemp fibers, and their blends with PLA fibers, subjected to field conditions, are investigated. This research explores the possible substitution of traditional agricultural polyethylene mulching agro foil with environmentally friendly biodegradable nonwoven mulches produced from blends of jute, hemp, and viscose fibers along with PLA fibers. The nonwoven mulches underwent a ten-month exposure to field conditions, showing varied degradation. The jute and hemp nonwoven mulches degraded completely within the test period, whereas their blends with PLA fibers exhibited slowed degradation. This study indicated that PLA fibers in blends with jute, hemp, and viscose mulches slowed degradation, impacting their structural integrity and tensile properties. The tensile properties of nonwoven mulches blended with 20% of PLA fibers increased the breaking forces after field exposure. Observations on structural changes through microscopy highlighted the structure maintenance in jute and hemp blends due to the non-degraded PLA fibers, contrasting the complete degradation of 100% jute and hemp mulches. A microscopic analysis revealed alterations in the fiber structure and density changes, particularly in viscose mulches and their blends with PLA fibers. Soil temperature variations were observed under different mulches; e.g., agro foil consistently exhibited higher temperatures compared to nonwoven mulches. Notably, the hemp and jute/PLA blend mulches showed slightly elevated temperatures, while the viscose-based mulches consistently revealed the lowest temperatures. Regarding soil moisture, the nonwoven mulches generally maintained higher moisture levels compared to the control field and agro foil from June to October. These findings suggest that nonwoven mulches effectively preserved soil moisture during critical growth periods, potentially positively impacting plant growth. The weed suppression capabilities varied among mulches, with hemp mulch initially displaying the lowest suppression ability in the first six months. The addition of 20% of PLA fibers in mulch blends with viscose, jute, and hemp notably improved the weed control capabilities. Understanding the impacts of field conditions on newly produced nonwoven mulches is crucial for optimizing mulch selection in agricultural practices to enhance soil conditions and weed management.

Designing a green poly(β-amino ester) for the delivery of nicotinamide drugs with biological activities and conducting a DFT investigation.

The environmentally friendly polymerization process was carried out using microwave irradiation without additional solvents or catalysts to produce poly(β-amino ester) (PβAE) which served as a drug delivery system. PβAE was synthesized through Michael addition polymerization of 1,4-butane diol diacrylate and piperazine. Swelling and biodegradation studies were conducted in various solvents and phosphate-buffered saline (PBS, pH 7.4) at 37 °C to evaluate the properties of the polymeric gel. The PβAE matrix demonstrated solubility enhancement for hydrophobic antimicrobial and antitumor-active nicotinamide derivatives (TEINH, APTAT, and MOAPM), controlling their release over 10 days in (PBS). The successful formation of free and loaded PβAE with nicotinamide active materials was confirmed by spectroscopic analysis including Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Optimization and physical descriptor determination via the DFT/B3LYP-631(G) basis set were performed to aid in the biological evaluation of these compounds with elucidation of their physical and chemical interaction between poly(β-amino ester) and nicotinamide drugs.

Study of biodegradability of chitosan-based antibacterial films

In one of our previous works, we obtained films based on chitosan, glycerol, and iron (III) cations. These films possess antibacterial properties, thus they can find applications in the food industry and medicine. In this study, we evaluated the biodegradability of previously described films by keeping them in soil for 4 weeks. For the control experiment, we utilized soil that underwent thermal treatment several times during the experiment. 4 out of 5 samples demonstrated mass loss slightly different from the mass loss in the control experiment (the difference in mass loss compared to the control for these samples did not exceed 3%). This might indicate that their mass loss was solely related to the dissolution process. The last sample, containing chitosan, iron chloride, glycerol, and a chitosanderived cation, exhibited a 40% decrease in mass, which is 26% more compared to the control experiment.

Biodegradation Study of Biomaterials Composed of Acrylated Epoxidized Soybean Oil: An In Vitro Study

Biodegradation Study of Biomaterials Composed of Acrylated Epoxidized Soybean Oil: An In Vitro Study

Systematic Intrinsic Biodegradation Studies of Crude Oil Contaminated Soil of Bdere Community in South-South, Nigeria

Crude oil pollution is a perennial environmental menace that has bedevilled the South-South ecosystem of Nigeria. This study was aimed at using gas chromatography-mass spectrometry (GC-MS) technique to investigate the biodegradation capabilities of nine bacterial cultures on crude oil residues in Bdere area in South-South, Nigeria. These microorganisms include Pseudomonas aeruginosa, Bacillus subtilis, Bacillus cereus, Micrococcus spp, Pseudomonas putida, Clostridium spp, Bacillus spp, Streptococcus spp, and Serratia spp. The results from the microbial-degraded samples were compared with an abiotic control. The findings reveal that the total petroleum hydrocarbon (TPH) in the microbial-treated samples was significantly attenuated compared to the control, confirming the microrganism's ability to degrade crude oil components. The primary degradation pathway involved biological oxidation of the aliphatic hydrocarbons, transforming them to primary alcohols, aldehydes, and fatty acid derivatives. Degradation was also observed across a wide range of short and long-chain alkanes, aromatic hydrocarbons, and polycyclic aromatic hydrocarbons (PAHs). However, some resistant compounds persisted, and certain degradation products inhibited the rate of further biodegradation. The generation of new metabolites and intermediates confirmed the effective microbial remediation. These findings expand our understanding of microbial degradation of hydrocarbons, offering potential strategies for environmental remediation of oil-contaminated sites.

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