Biodegradable Dissolved Organic Carbon Research Articles

Insights into dissolved organic carbon biodegradation process and influencing factors in shallow lakes in a metropolitan, China

Lakes perform a pivotal role in both regional and global carbon cycles. However, seasonal variations and drivers of dissolved organic carbon (DOC) biodegradability are poorly understood in shallow urban lakes. To address this knowledge gap, a 28-day incubation experiment in urban shallow lakes was carried out to quantify proportion of biodegradable DOC (%BDOC) and temperature sensitivity (Q10 value). %BDOC and Q10 value were significantly higher in the wet season (%BDOC20: 14.48% ± 7.92%, %BDOC30: 17.61% ± 8.98%, Q10:1.32 ± 0.62) over the dry season (%BDOC20: 10.35% ± 6.98%, %BDOC30: 11.59% ± 7.36%, Q10: 1.20 ± 0.65). The absorbance at 350nm (a350) followed a decreasing-increasing-decreasing trend, while a250 / a365 (E2:E3) had a contrasting trend to a350 during the incubation. Three (dry season) and five (wet season) fluorescent components were captured by parallel factor analysis (PARAFAC). The components C2 (terrestrial humic-like) and C3 (tryptophan-like) were largely biodegraded on day 28 in the wet season. The %BDOC was positively related to total dissolved nitrogen (TDN), total dissolved phosphorus (TDP) and fluorescence index (FI), but negatively related to humification index (HIX) during the dry season. However, in the wet season, the %BDOC was positively related to TDN, TDP and DOC. The results show that the controls of DOC biodegradation shift from the combined DOC quality and nutrients (dry season) to DOC quantity (wet season). The study sheds light on the drivers of BDOC seasonality, and underscores the significance of both nutrients and DOC in understanding carbon-geochemistry within lake ecosystems.

Role of BP-ANN in simulating greenhouse gas emissions from global aquatic ecosystems via carbon component-environmental factor coupling

Inland waters (IW), estuarine areas (EA), and offshore areas (OA) function as aquatic systems in which the transport of carbon components results in the release of greenhouse gases (GHGs). Interconnected subsystems exhibit a greater greenhouse effect than individual systems. Despite this, there is a lack of research on how carbon loading and its components impact GHG emissions in various aquatic systems. In this study, we analyzed 430 aquatic sites to explore trade-off mechanisms among dissolved organic carbon (DOC), particulate organic carbon, dissolved inorganic carbon (DIC), and GHGs. The results revealed that IW emerged as the most significant GHG source, possessing a comprehensive global warming potential (GWP) of 0.78 ± 0.08 (10−2 Pg CO2-ep ha−1 year−1) for combined carbon dioxide, methane, and nitrous oxide. This surpassed the cumulative potentials of EA and OA (0.35 ± 0.05 (10−2 Pg CO2-ep ha−1 year−1)). Additionally, structural equation modeling indicated that GHG emissions resulted from a combination of carbon component loading and environmental factors. DOC exhibited a positive correlation with GWPs when influenced by biodegradable DOC. Total alkalinity and pH influenced DIC, leading to elevated pCO2 in aquatic systems, thereby enhancing GWPs. Predictive modeling using backpropagation artificial neural networks (BP-ANN) for GWPs, incorporating carbon components and environmental factors, demonstrated a good fit (R2 = 0.6078, RMSEaverage = 0.069, p > 0.05) between observed and predicted values. Enhancing the estimation of aquatic region feedback to GHG changes was achieved by incorporating corresponding water quality parameters. In summary, this study underscores the pivotal role of carbon components and environmental factors in aquatic regions for GHG emissions. The application of BP-ANN to estimate greenhouse effects from aquatic regions is highlighted, providing theoretical and experimental support for future advancements in monitoring and developing policies concerning the influence of water quality on GHG emissions.

Concentration and compositional controls on degradation of permafrost‐derived dissolved organic matter on the Qinghai–Tibetan Plateau

AbstractUnderstanding the fate of permafrost‐derived dissolved organic matter (DOM) is critical for unraveling its role in carbon cycling. However, it remains unclear whether the high lability of permafrost‐derived DOM can be attributed to intrinsic chemical properties or elevated carbon concentrations. We investigated the dynamics of permafrost DOM from the Qinghai–Tibetan Plateau using both biodegradation and photodegradation experiments. Biodegradation and photodegradation of permafrost‐derived DOM exhibited distinct qualitative preferences for specific chemical groups (i.e., peptide‐like and aromatics, respectively). Notably, reducing the initial concentration of dissolved organic carbon (DOC) by half and a quarter resulted in shifts in biodegradable DOC content from 11.2% to 11.5% and 8.5%, respectively, accompanied by a corresponding decrease in the biodegradation rate from 0.11 to 0.06 and 0.03. This insight highlights the importance of recognizing the interplay between DOM quality and concentration and bears broader significance for our understanding of the fate of permafrost‐derived DOM in natural ecosystems.

Agricultural Land‐Use Increases Carbon Yields in Lowland Streams of the Congo Basin

AbstractAs the dominant mode of deforestation in the Congo Basin, shifting agriculture is expected to increase with the projected four‐fold population growth for the region by 2,100. To assess how this land‐use change will affect the export of carbon (C) to rivers in a typical lowland forest ecosystem, we studied paired watersheds near Kisangani, Democratic Republic of the Congo. Two streams, one draining an intact forest (Forest) and one draining an agricultural landscape (Ag), were gauged, equipped with sensors, and sampled fortnightly for one year. Annual average specific discharge was 1.4 mm d−1 (+76%) higher in the Ag compared to the Forest. Average annual dissolved organic C (DOC) and particulate organic C (POC) concentrations were 5.2 mg L−1 (+163%) and 1.3 mg L−1 (+81%) higher in the Ag stream, which, along with the higher discharge, resulted in 8.3 (+410%) and 2.4 g C m−2 yr−1 (+97%) larger C yields, respectively. Baseflow dissolved inorganic carbon, carbon dioxide, and methane yields were also higher in the Ag stream. Despite the higher yields of organic C (OC), the composition of OC did not differ significantly. Carbon to nitrogen ratios, along with isotopic signatures, revealed that both streams contained young, semi‐degraded organic matter derived from C3 vegetation. Correspondingly, biodegradable DOC (BDOC) proportions did not differ between the streams, although the Ag stream yielded more total BDOC. These results show that agricultural land‐use likely exports a greater proportion of Net Primary Productivity (NPP) to aquatic ecosystems, which may affect both C storage in soils and the proportion of gross PP that is ultimately respired.

Ozonation and Changes in Biodegradable Organic Substances in Drinking Water Treatment: The Future of Green Technology

Studies were carried out to assess changes in biodegradable dissolved organic carbon (BDOC) and assimilable organic carbon (AOC) in groundwater and surface waters after two processes: ozonation and ozonation/UV. The tested water was in contact with O3 firstly for 4 and secondly for 15 min. Three doses of disinfectant were used: 1.6 mg/L, 5.0 mg/L, and 10.0 mg/L. The UV radiation time was 10 and 30 min. The greatest change in AOC and BDOC for groundwater was observed at an O3 dose of 10.0 mg/L and a contact time of 15 min, by 400 and 197%, respectively. On the other hand, for surface water, it was shown that after the ozonation/UV process, the AOC and BDOC content decreased after both 10 and 30 min of radiation in comparison to the water after ozonation. The AOC content decreased by 33% and 22%, respectively, and the BDOC content by 27% and 31%, respectively. The results obtained in this study provide new information on the effect of different ozonation conditions and the combined method on the level of biodegradable organic fraction of water. It is recommended that BDOC and AOC should be monitored in Poland as routine indicators during the preparation of drinking water.

Effect of flow fluctuation on water pollution in drinking water distribution systems

The detachment of biofilm caused by changes in hydraulic conditions is an essential reason for the pollution of water in the drinking water distribution system (DWDS). In this research, the effect of flow fluctuation on bulk water quality was studied. The turbidity, iron concentration, manganese concentration, the total number of bacteria, biodegradable dissolved organic carbon (BDOC), bacterial community structure, and pathogenic genes in bacteria of bulk water were analyzed. The results indicate that the detachment of biofilm caused by fluctuant flow and reverse flow (especially instant reverse flow) can lead to the pollution of water. Throughout the entire experimental period, the turbidity under fluctuant flow velocity is 4.92%∼49.44% higher than that under other flow velocities. BDOC concentration is 5.68%∼53.99% higher than that under low and high flow velocities. The flow fluctuation increases bacterial regrowth potential (BRP) and reduces the biological stability of the bulk water. Low flow velocity is more conducive to the expression of pathogenic functional genes. In the short term, the water quality under low flow velocity is the best. Nevertheless, in a long-term operation (about seven days later), the water quality under high flow velocity is better than that under other flow velocities. This research brings new knowledge about the fluctuant hydraulic conditions on the bulk water quality within the DWDS and provides data support for stable drinking water distribution.

Organic matter biofiltration performance modeling: Influence of influent water quality, operating conditions, and biomass

The impact of source water dissolved organic matter (DOM) origin, empty bed contact time (EBCT), temperature, and pretreatment methods on biofiltration performance was evaluated and predictive models based on experimental data were developed. Three DOM source water types, terrestrial, microbial, and treated wastewater (WW) effluent, were utilized. A model was developed to predict biofilter performance for dissolved organic carbon (DOC) removal based on the influent biodegradable DOC (BDOC) fraction, a single active biomass measurement from the top of the filter and the filter EBCT. A biomass distribution model was developed to predict total active biomass throughout the filter based on a single biomass measurement from the top of the filter. The measured BDOC fractions were 21 % for the nonWW impacted source waters, 36 % for the WW effluents and 62 % for the ozonated WW effluents. At an EBCT of 15 min, biofilters removed between 7 and 21 % of the DOC (19 to 50 % for BDOC) depending on the DOM type and use of ozonation. When the EBCT decreased to 5 min DOC removal decreased by 40 % and when increased to 30 min removal increased by 42 %. When the temperature decreased from 22 °C to 6 °C DOC removal was 33 % lower and when increased to 28 °C removal was 42 % higher. ATP values were found to be a function of temperature and DOM origin, as the average ATP values from the WW effluent biofilters were almost double that of the non-WW impacted sources and pre-ozonation of the WW effluent yielded values three times higher. The model was applied to the results of 27 different biofilter runs at three EBCTs yielding one distinct rate constant for the non-WW impacted source waters and one rate constant for the WW effluents. The model was successfully applied to the results of 19 filter runs from the literature and to those from a pilot plant over 6 months of operation.

Biodegradable dissolved organic carbon profiling reveals capacity of carbon‐based potable reuse treatment over a range of operating conditions

AbstractBiological treatment is gaining ground as a means to enhance removal of total organic carbon (TOC) as part of a multi‐barrier treatment train for water reuse. Here we applied biodegradable dissolved organic carbon (BDOC) analysis to evaluate the extent of removal of various TOC fractions through a pilot‐scale water reuse train employing flocculation/sedimentation, ozone, biologically active carbon (BAC), and granular activated carbon (GAC). BDOC analysis highlighted GAC and ozone treatments as critical to non‐biodegradable dissolved organic carbon removal and the need to optimize BAC performance to maximize GAC adsorption capacity. BDOC analysis was further applied to benchmark process performance to operational conditions, such as empty bed contact time (EBCT), occurrence of nitrification, and operational upsets. The lower EBCT proved to be less resilient to nonideal conditions. Overall, BDOC analysis proved an asset for understanding and improving operation of ozone/BAC/GAC treatments for water reuse.

Impact of invertebrates on water quality safety and their sheltering effect on bacteria in water supply systems

Invertebrates in drinking water not only affect human health, but also provide migration and shelter for pathogenic microorganisms. Their residues and metabolites also produce DBPs (disinfection by-products), which have adverse effects on the health of residents. In this study, the contributions of the rotifers and nematodes to the BDOC (biodegradable dissolved organic carbon), BRP (bacterial regrowth potential) and DBPs in drinking water were explored, and the sheltering effects of chlorine-resistant invertebrates on indigenous bacteria and pathogenic bacteria were studied, and the health and safety risk of invertebrates in drinking water was also assessed. The contributions of rotifer BAPs (biomass-associated products), UAPs (utilization-associated products) of rotifer, and nematode BAPs to the BRP were 46, 1240, and 24 CFU/mL. Nematodes were found to have a sheltering effect on indigenous bacteria and pathogenic bacteria, allowing them to resist chlorine disinfection and UV (ultraviolet) disinfection. When subjected to a UV dose of 40 mJ/cm2, the inactivation rates of indigenous bacteria and three pathogenic bacteria decreased by 85% and 39–50% when bacteria were sheltered by the living nematodes; while decreased by 66% and 15–41% when they were sheltered by nematode residue. The safety risk posed by invertebrates in the drinking water was mainly due to their ability to promote bacterial regeneration and carry bacteria. This study aims to provide a theoretical basis and technical support for the risk control of invertebrates' pollution, and provides references for ensuring the safety of drinking water and formulating standards for the levels of invertebrates in drinking water.

Advanced oxidation processes for removal of monocyclic aromatic hydrocarbon from water: Effects of O3/H2O2 and UV/H2O2 treatment on product formation and biological post-treatment

Several oxidative treatment technologies, such as ozonation or Fenton reaction, have been studied and applied to remove monocyclic hydroaromatic carbon from water. Despite decades of application, little seems to be known about formation of transformation products while employing different ozone- or ∙OH-based treatment methods and their fate in biodegradation. In this study, we demonstrate that O3/H2O2 treatment of benzene, toluene, ethylbenzene (BTE), and benzoic acid (BA) leads to less hydroxylated aromatic transformation products compared to UV/H2O2 as reference system – this at a similar ∙OH exposure and parent compound removal efficiency. Aerobic biodegradation tests after oxidation of 0.15 mM BA (12.6 mg C L−1 theoretical DOC) revealed that a less biodegradable DOC fraction > 4 mg C L−1 was formed in both oxidative treatments compared to the BA control. No advantage of ozonation over UV/H2O2 treatment was observed in terms of mineralization capabilities, however, we detected less transformation products after oxidation and biodegradation using high-resolution mass spectrometry. Biodegradation of BA that was not oxidized was more complete with minimal organic residual. Overall, the study provides new insights into the oxidation of monocyclic aromatics and raises questions regarding the biodegradability of oxidation products, which is relevant for several treatment applications.

Seasonal and Spatial Variations of Dissolved Organic Matter Biodegradation along the Aquatic Continuum in the Southern Taiga Bog Complex, Western Siberia

The inland aquatic ecosystems play a significant role in the global carbon cycle, owing to the metabolism of terrestrially derived organic matter as it moves through fluvial networks along the water continuum. During this transport, dissolved organic matter (DOM) is microbial processed and released into the atmosphere, but the degree and intensity of this processing vary greatly both spatially and temporally. The Western Siberian Lowlands is of particular interest for a quantitative assessment of DOM biodegradation potential because the global areal-scale effects of DOM biodegradation in abundant surface organic-rich waters might be the highest in this region. To this end, we collected water samples along a typical aquatic continuum of the Bakchar Bog (the north-eastern part of the Great Vasyugan Mire) and, following standardized protocol, conducted an experimental study aimed at characterizing the seasonal and spatial variability of dissolved organic carbon (DOC) biodegradability. The biodegradable DOC fraction (BDOC) over the exposure incubation period ranged from 2% to 25%. The natural aquatic continuum “mire–forest–stream–river” demonstrated the systematic evolution of biodegradable DOC among the sites and across the seasons. The highest biodegradation rates were measured during spring flood in May and decreased along the continuum. The maximum possible CO2 production from DOM yielded the maximum possible flux in the range of 0.1 and 0.2 g C-CO2 m−2 day−1 d, which is an order of magnitude lower than the actual net CO2 emissions from the inland waters of the WSL. This study suggests that although the biodegradation of the humic waters of the WSL can sizably modify the concentration and nature of the DOM along the aquatic continuum, it plays only a subordinary role in overall C emissions from the lakes and rivers of the region.

Biodegradable Dissolved Organic Carbon (BDOC) Removal from Micro-Polluted Water Source Using Ultrafiltration: Comparison with Conventional Processes, Operation Conditions and Membrane Fouling Control.

The biodegradable dissolved organic carbon (BDOC) in micro-polluted water sources affects the drinking water quality and safety in the urban water supply. The conventional technology of "coagulation-sedimentation-filtration" in a water plant located in the lower reaches of the Yangtze River removed dissolved organic carbon (DOC) with a molecular weight (MW) > 30 kDa effectively, but the BDOC elimination only ranged 27.4-58.1%, due to their predominant smaller MW (<1 kDa), leading to a high residual BDOC of 0.22-0.33 mg/L. To ensure the biological stability of drinking water, i.e., the inability to support microbial growth (BDOC < 0.2 mg/L), a pilot-scale ultrafiltration process (UF, made of aromatic polyamide with MW cut-off of 1 kDa) was operated to remove BDOC as an advanced treatment after sand-filtration. Results showed the membrane flux decreased with the increase in the influent BDOC concentration and decrease in operating pressure. With an operating pressure of 0.25 MPa, the BDOC removal by UF reached 80.7%, leading to a biologically stable BDOC concentration of 0.08 mg/L. The fouling of the membrane was mainly caused by organic pollution. The H2O2-HCl immersion washing method effectively cleaned the membrane surface fouling, with a recovery of membrane flux of 98%.

A distinctive mode of dissolved organic carbon biodegradation in karst lakes and reservoirs: Evidence from trophic controls and compositional transformations

Lakes and artificial reservoirs present as funnels for global and regional carbon cycling. However, limited information is available about dissolved organic carbon (DOC) bioavailability, especially for its trophic controls and compositional transformations in widely distributed karst waters. Here we examined DOC biodegradation (%BDOC), temperature sensitivity (Q10 value), nutrients and their stoichiometric ratios, as well as DOC composition over a 28-day laboratory incubation experiment in karst lakes and reservoirs. Fluorescence, Fourier transform infrared (FTIR) and UV–Visible spectroscopy were employed to trace DOC variability. We found that %BDOC ranged between 1.17% and 66.6% (median: 30.1%) at 15 °C and 5.95%–68.5% (median: 31.0%) at 30 °C, respectively. The Q10 values were mainly distributed between 0.75 and 1.50, suggesting a large amount of degraded substrate with low activation energy. Aquatic DOC and total dissolved nitrogen (TDN) were tightly linked to the %BDOC, whereas significant phosphorus (P) limitation (TDN:TDP > 25) did not induce discernible correlations. Carbohydrates as a dominant contributor to biodegradable DOC (BDOC) largely drove DOC remineralization. The incomplete degradation of humic-like fractions increased the relative abundance of fulvic acid, tyrosine-like and tryptophan-like fractions. A distinctive mode as biological utilization of extreme active and inactive components could be summarized in the karst lakes and reservoirs. These findings hope to supplement special mechanisms of DOC transformation and fate in the context of climate change mitigation for the UN Sustainable Development Goals.

Distributive Features of Dissolved Organic Carbon in Aquatic Systems in the Source Area of the Yellow River on the Northeastern Qinghai–Tibet Plateau, China

Dissolved organic carbon (DOC) is the main participant in carbon cycles through water pathways. Recent studies have highlighted the roles of aquatic systems in landscape and watershed carbon budgets. This study is based on 261 samples collected between 2016 and 2017, from individual water types (e.g., river/stream, lake/pond, icing/spring, snow/rain, groundwater/ice, and others) in the source area of the Yellow River (SAYR). These samples were analyzed for examining the distributive features of DOC in aquatic systems, especially in relation to environmental factors. It shows that: 1) DOC concentrations in permafrost-related waters (7.2–234.4 mg C·L−1) were often the highest among all aquatic DOC sources (lakes/ponds: 21.3 ± 34.1 mg C·L−1, rivers/streams: 4.3 ± 3.7 mg C·L−1, and groundwater: 1.8 ± 1.4 mg C·L−1); 2) the seasonality of riverine DOC showed declining features in 2016 and high in summer/autumn, followed by a spring freshet in 2017, and a close association with intra-annual precipitation modes; 3) the main controls of aquatic DOC are permafrost presence, precipitation, and NDVI, and they contribute to 38% of variances of environmental variables in affecting variations in aquatic DOC in the SAYR; and 4) a literature review on biodegradable DOC (BDOC) of varied aquatic DOC pools indicates the highest DOC concentrations (48–1,548 mg C·L−1) and BDOC (23–71%) of ground-ice meltwater. Thus, we suggest that in the SAYR, permafrost dynamics dominate aquatic DOC distribution, and permafrost thaw may alter aquatic DOC budgets, eventually becoming an additional source for atmospheric carbon emissions.

Antibiotic enhances the spread of antibiotic resistance among chlorine-resistant bacteria in drinking water distribution system

The extensive use of antibiotics leads to the occurrences of antibiotic resistance genes (ARGs) in aquatic environment. As an emerging environmental pollutant, its pollution in aquatic environment has aroused widespread concern. However, the residues of antibiotics and antibiotic resistance genes in drinking water distribution system were barely reported up to now. Here, we studied the correlation and coordination between chlorine resistance mechanism and antibiotic resistance mechanism of chlorine-resistant bacteria. Antibiotics induce the resistance of chlorine-resistant bacteria (CRB) to NaClO, so that low-dose disinfectants can not inactivate CRB. We put forward a strategy to control the growth of CRB by controlling the concentration of biodegradable dissolved organic carbon (BDOC) in the front section of the water network. Moreover, We screened two strains of chlorine-resistant bacteria with different antibiotic resistance after mixed culture, the results showed that antibiotic resistance could spread horizontally among different kinds of bacteria. Then, the non-pathogenic bacteria can be used as a carrier, causing the pathogen to become resistant to antibiotic, and ultimately pose harm to human health. Generally, the antibiotic, antibiotic resistant genes, and the chlorine disinfectants added in water treatment plants will interact with bacteria in the water supply pipe network, which causes pollution to drinking water.

Reservoirs change pCO2 and water quality of downstream rivers: Evidence from three reservoirs in the Seine Basin

The global increase in the construction of reservoirs has drawn attention given its documented hydrological and biogeochemical impacts on downstream rivers; however, the impact of reservoirs on downstream pCO2 (partial pressure of carbon dioxide) is still poorly understood. To evaluate these impacts, the interactions between reservoirs and their corresponding upstream and downstream rivers were analyzed for three reservoirs in the Seine Basin based on monthly measurement during two hydrological years. The seasonal variations of water quality in the reservoirs were mainly driven by the entering water and the biogeochemical processes occurring in the reservoirs. Our results unravel the crucial role of reservoir in downstream water quality, which significantly increased DOC (dissolved organic carbon) and BDOC (biodegradable DOC) concentrations, while lowered DSi (dissolved silica) concentrations during emptying period (p < 0.01). Furthermore, the impacts of reservoirs on the annual fluxes of DOC, BDOC, and DSi were quantified and suggested that the three reservoirs respectively increased 20% and 23% of annual fluxes of DOC and BDOC, while decreased 33% of annual DSi fluxes in their downstream rivers. Additionally, the reservoirs significantly decreased downstream riverine pCO2 (p < 0.01), and enhanced the gas transfer coefficient of CO2 in downstream rivers by 1.3 times during the emptying period, which highlights the necessity to consider the potential impact of reservoirs on riverine CO2 emissions. Overall, our results highlight the importance of combining biogeochemical and hydrological characteristics to understand the impacts of reservoirs on downstream rivers, and emphasize the need of similar studies under the current context of increasing reservoir constructions.

Characterization of natural organic matter in South African drinking water treatment plants: Towards integrating ceramic membrane filtration.

This work presents the first comprehensive investigation of natural organic matter (NOM) fraction removal using ceramic membranes in South Africa. The rate of removal of bulk NOM (measured as UV254 and DOC % removal), the biodegradable dissolved organic carbon (BDOC) fraction, polarity-based fractions, and fluorescent dissolved organic carbon (FDOM) fractions was investigated from water abstracted from drinking water treatment plants (WTPs) in South Africa. Further, mechanisms of ceramic membrane fouling by waters of South Africa were studied. Ceramic membranes removed more than 80% DOC from samples from coastal WTPs, whereas for inland plants, the removal was between 60% and 75% of DOC. FDOM was removed to at least 80% regardless of the site of the plant. The BDOC removal by the ceramic membranes was above 85%. The hydrophobic fraction was the most amenable to removal by ceramic membranes regardless of the site of sample abstraction (above 60% for all sites). The freshness index (β:α) correlated strongly to UV254 removal (R2 = 0.96), thus UV254 removal can serve as a proxy for the susceptibility to removal of such class of NOM by ceramic membranes. This investigation demonstrated that ceramic membranes could be a valuable technology if integrated into the existing WTPs. PRACTITIONER POINTS: The removal of bulk parameters by ceramic membrane was greater than unit conventional processes used in all the sampled water treatment plants. The hydrophobic polarity-based fraction of NOM was the most amenable to removal by ceramic membranes regardless of the site of the WTP. Polarity-based fractions, aromaticity, and initial DOC had a combined influence on the removal of organic matter by ceramic membranes as explained by principal component three.

Regrowth potential of chlorine-resistant bacteria in drinking water under chloramination

The regrowth of chlorine-resistant bacteria in drinking water can deteriorate water quality. The study evaluated the relationship between organic carbon and the regrowth potential of chlorine-resistant bacteria remaining in chloraminated water samples. The results showed that the community structure of bacteria changed with the increase of chloramine dosage. The order in which organic carbon utilized by bacteria was affected by the composition of bacterial community. The biodegradable dissolved organic carbon (BDOC), assimilable organic carbon (AOC), bacterial regrowth potential (BRP) and total cell concentration (TCC) in cultivated water sample after disinfection with 1.8 mg/L chloramine increased form 0.22 mg/L, 33.68 µg/L, 2.70 × 105 cells/mL and 3.48 × 104 cells/mL before cultivation to 1.20 mg/L, 193.90 µg/L, 4.74 × 105 cells/mL and 1.46 × 105 cells/mL, respectively. The increase of TCC did not result in the decrease of BDOC, AOC and BRP in the cultivated water samples. The results showed that other biodegradable organic carbon in chloraminated water samples assimilated by residual chlorine-resistant bacteria besides AOC, BDOC, and organic carbon assimilated by indigenous bacteria. AOC, BDOC, and BRP indicators used to characterize the biostability of drinking water were not enough to accurately assess the regrowth potential of chlorine-resistant bacteria remaining in drinking water. It is suggested to supplement the index of TCC in cultivated water samples, which might be able to more accurately evaluate the regrowth potential of chlorine-resistant bacteria remaining in drinking water.

The impact of heterotrophic bacteria on recalcitrant dissolved organic carbon formation in a typical karstic river

Recalcitrant dissolved organic carbon (RDOC) resulting from microbial carbon (MCPs) holds promise as a relatively long-term natural carbon sink in marine environments. However, the RDOC formation mechanism remains uncertain in terrestrial aquatic systems. To determine the microbial impacts on autochthonous dissolved organic carbon (DOC), RDOC formation, and the critical influencing bacteria species, spatial changes in hydrochemistry, carbon isotopes, and microbial diversity were investigated in water samples from the karstic Lijiang River, southwest China. Samples were collected at various locations along the river system in May and July 2017. The biodegradable DOC (BDOC), RDOC, soil sourced DOC (SDOC), submerged aquatic vascular plant sourced DOC (PDOC) and microbial sourced DOC (MDOC) were calculated using the in-situ microbial incubation method, stable carbon isotopes and C/N ratio. RDOC accounted for 67% to 93% of DOC concentrations, measuring 1.3 mg/L and 1.2 mg/L in May and July, respectively. In May, BDOC concentrations increased by 0.05 mg/L from 0.18 mg/L to 0.23 mg/L, but decreased by 0.43 mg/L from 0.66 mg/L to 0.23 mg/L in July. The spatiotemporal variation of BDOC indicated photosynthesis was the main BDOC source and induced high autochthonous DOC formation, especially in May. However, RDOC was the dominant accumulation component in Lijiang River. MDOC increased by 0.86 mg/L from 0 to 0.86 mg/L in May and 0.78 mg/L from 0.10 mg/L to 0.88 mg/L in July, which was the dominant accumulated DOC and RDOC component. The abundance of Sporichthyaceae accounted for 3.4%–22.6% in May and Novosphingobium accounted for 3.5%–34.0% in July. These were the critical bacteria species induced MDOC formation, which were confirmed by their abundances in KEGG pathway modules determined by PICRUAST2. These results demonstrate that heterotrophic bacteria dominate autochthonous DOC and RDOC formation in the karst surface river, which is valuable for understanding organic carbon cycling in karstic aquatic systems.

Effect of ozonation on the biodegradability of urban wastewater treatment plant effluent

The present work aimed to study the effect of ozonation on the organic sum parameters linked to enhanced biodegradability. Laboratory experiments were conducted with the effluent of four Austrian urban wastewater treatment plants with low food to microorganism ratios and different matrix characteristics. Biochemical oxygen demand over 5 days (BOD5) was measured before ozonation and after application of different specific ozone doses (Dspec) (0.4, 0.6 and 0.8 g O3/g DOC). Other investigated organic parameters comprised chemical oxygen demand (COD), dissolved organic carbon (DOC), UV absorption at 254 nm (UV254), which are parameters that are applied in routine wastewater analysis. Carbamazepine and benzotriazole were measured as reference micropollutants. The results showed a dose-dependent increase in biological activity after ozonation; this increase was linked to the enhanced biodegradability of substances that are recalcitrant to biodegradation in conventional activated sludge treatment. The highest relative change was determined for BOD5, which already occurred between 0 and 0.4 g O3/g DOC for all samples. Increasing the Dspec to 0.6 and 0.8 g O3/g DOC resulted in a less pronounced increase. DOC was not substantially decreased after ozonation, which was consistent with a low reported degree of mineralization, while partial oxidation led to a quantifiable decrease in COD (7 to 17%). Delta UV254 and the decline in specific UV absorption after ozonation clearly correlated with Dspec. In contrast, for COD and biodegradable DOC (BDOC), a clear dose-response pattern was identified only after exposure to BOD5 measurement. Indications for improved biodegradability were further supported by the rise in the BOD5/COD ratio. The results indicated that subsequent biological processes have a higher degradation potential after ozonation. The further reduction in biodegradable organic carbon emission by the combination of ozonation and biological post treatment represents another step towards sustainable water resource management in addition to micropollutant abatement.