A distinctive mode of dissolved organic carbon biodegradation in karst lakes and reservoirs: Evidence from trophic controls and compositional transformations

Abstract

Lakes and artificial reservoirs present as funnels for global and regional carbon cycling. However, limited information is available about dissolved organic carbon (DOC) bioavailability, especially for its trophic controls and compositional transformations in widely distributed karst waters. Here we examined DOC biodegradation (%BDOC), temperature sensitivity (Q10 value), nutrients and their stoichiometric ratios, as well as DOC composition over a 28-day laboratory incubation experiment in karst lakes and reservoirs. Fluorescence, Fourier transform infrared (FTIR) and UV–Visible spectroscopy were employed to trace DOC variability. We found that %BDOC ranged between 1.17% and 66.6% (median: 30.1%) at 15 °C and 5.95%–68.5% (median: 31.0%) at 30 °C, respectively. The Q10 values were mainly distributed between 0.75 and 1.50, suggesting a large amount of degraded substrate with low activation energy. Aquatic DOC and total dissolved nitrogen (TDN) were tightly linked to the %BDOC, whereas significant phosphorus (P) limitation (TDN:TDP > 25) did not induce discernible correlations. Carbohydrates as a dominant contributor to biodegradable DOC (BDOC) largely drove DOC remineralization. The incomplete degradation of humic-like fractions increased the relative abundance of fulvic acid, tyrosine-like and tryptophan-like fractions. A distinctive mode as biological utilization of extreme active and inactive components could be summarized in the karst lakes and reservoirs. These findings hope to supplement special mechanisms of DOC transformation and fate in the context of climate change mitigation for the UN Sustainable Development Goals.