Bioconjugation Research Articles

MULTIFUNCTIONAL NANO–BIO MATERIALS WITHIN CELLULAR MACHINERY

Functional nanoscale materials that possess specific physical or chemical properties can leverage energy transduction in vivo. Once these materials integrate with biomolecules they combine physical properties of inorganic material and the biorecognition capabilities of bio-organic moieties. Such nano-bio hybrids can be interfaced with living cells, the elementary functional units of life. These nano-bio systems are capable of bio-manipulation or actuation via altering intracellular biochemical pathways. Thus, nano-bio conjugates are appealing for a wide range of applications from the life sciences and nanomedicine to catalysis and clean energy production. Here we highlight recent progress in our efforts to develop smart nano-bio hybrid materials, and to study their performance within cellular machinery under application of external stimuli, such as light or magnetic fields.

Modification and bioconjugation of targeted ultrasound contrast agents

靶向超声分子成像的出现使超声成像进入一个新的发展阶段,它是以靶向超声造影剂作为示踪剂,通脱造影剂与靶组织异表达分子的有效结合,采用对比超声产生靶组织分子水平显影的一种新型超声成像技术。

A REVIEW ON PHARMACEUTICAL AND NON PHARMACEUTICAL APPLICATIONS OF DENDRIMER

A dendrimer is generally described as a macromolecule, which is characterized by its highly branched 3D structure that provides a high degree of surface functionality and versatility. Dendrimers are a unique class of polymers which play an important role in emerging nanotechnology. Targeted drug delivery is one of the most attractive potential applications of Dendrimers. The present review brie?y describes about dendrimer synthesis strategies, dendrimer functionlisation properties which having crucial importance and their potential applications such as carrier molecule, Multivalent Diagnostics for MRI, Sensors, Multivalent Bioconjugate, Light harvesting, catalysis, artificial enzymes, coatings, inks and other application. As part of our recent surveys into patent trends in nanotechnology, we discovered an explosion in dendrimer patenting which should contribute to the momentum for their commercialization, particularly in nanotechnology. Rationally and precisely designed dendrimer-based carriers may realize an accurate delivery of drugs to the target site and lead to an enhanced efficacy of drugs.

Novel water-soluble bioconjugates of chitosan/aspartame diketopiperazine and their green-fabrication

Novel water-soluble bioconjugates of chitosan/aspartame diketopiperazine and their green-fabrication

Azide-alkyne coupling: a powerful reaction for bioconjugate chemistry.

Azide-alkyne coupling: a powerful reaction for bioconjugate chemistry.

Carbodi-imide coupling of enzymes to the reversibly soluble insoluble polymer Eudragit S-100.

The coupling of proteins and enzymes to soluble-insoluble polymers by carbodi-imide can be performed by using numerous variations of the protocol. This protocol has been investigated for the coupling of five different enzymes, namely wheatgerm acid phosphatase, beta-glucosidase, beta-galactosidase, trypsin and xylanase, to an enteric methacrylate polymer Eudragit S-100. The following results were found. (1) The activity of the bioconjugate was critically dependent on the physical state of the polymer and the pH of the coupling reaction. For example, in the case of wheatgerm acid phosphatase, the activity of the bioconjugate was 49% when coupling was performed at pH 7.2 and 67% when coupling was performed at pH 4. 5. With beta-galactosidase the corresponding values were 57% and 23% and with beta-glucosidase they were 57% and 52% respectively. (2) In some cases, such as beta-glucosidase and beta-galactosidase, it might be necessary to remove excess carbodi-imide before the addition of the enzyme to the activated matrix. (3) In most of the cases investigated, a sig-nificant amount of the enzyme (more than 90%) could be bound to the matrix merely by adsorption. (4) More importantly, after the carbodi-imide coupling procedure, a sufficient fraction of the bound enzyme could be eluted off the matrix, indicating that this was merely adsorbed and not covalently coupled.

Bioconjugates may recruit drugs to bone

Bioconjugates may recruit drugs to bone