Biochemical Reactions Research Articles

Ultrafast T1-T1ρ NMR for Correlating Different Motional Regimes of Molecules.

Nuclear magnetic resonance (NMR) relaxation times provide detailed information about molecular motions and local chemical environments. Longitudinal T1 relaxation time is most often sensitive to relatively fast, nano- to picosecond ranges of molecular motion. Rotating frame T1ρ relaxation time reflects a much slower, micro- to millisecond range of motion, and the motional regime can be tuned by changing spin-lock field strength. Conventional methods for measuring T1 and T1ρ relaxation times are time-consuming, since experiments must be repeated many times with incremented magnetization recovery or decay delay. In this work, we introduce two novel and efficient NMR methods to correlate the T1 and T1ρ relaxation times. The first method, IR-SPICY, combines the conventional T1 inversion recovery (IR) with the single-scan T1ρ detection-based spin-lock cycle (SPICY). The second method, ultrafast (UF) IR-SPICY, allows measurement of whole two-dimensional T1-T1ρ correlation data in a single scan, in a couple of seconds, based on spatial encoding of the T1 dimension. We demonstrate the performance of the methods by studying relaxation of water in porous silica and hydrogel samples, latter acting as a model of the articular cartilage extracellular matrix. The methods allow correlating different molecular motional regimes, potentially providing unprecedented information about various chemical and biochemical systems, such as structures and fluid dynamics in porous materials, macromolecular changes in tissues, and protein dynamics. One to three orders of magnitude shortened experiment time enable the studies of changing or degrading samples. Furthermore, the single-scan approach may significantly facilitate the use of modern nuclear-spin hyperpolarization techniques to enhance the sensitivity of T1-T1ρ measurements by several orders of magnitude.

Thyme and Oregano Oil Potential Therapeutics against Malathion Toxicity through Biochemical, Histological, and Cytochrome P450 1A2 Activities in Male Wistar Rats.

The widespread use of malathion may offer several hazards to humans and animals; additionally, many medicinal plants provide what is known as a broad antitoxicity treatment. This study was carried out to investigate hazardous biochemical and histological reactions to MOP and evaluate the effectiveness of TEO and OEO essential oils in restoring normal physiological conditions after MOP exposure by measuring enzyme-specific activity for Cytochrome P450 1A2 (CYP1A2). One hundred and twenty rats were divided into six groups of twenty animals each: (i) C - MOP served as the control group, (ii) C + MOP treated with 5 mg/kg/BW of Malathion-D10, (iii) TEO treated with 100 mg/kg/BW of oregano essential oil, (iv) TEO treated with 100 mg/kg/BW of thyme essential oil, (v) MOP + OEO treated with 5 mg/kg/BW of Malathion-D10 and 100 mg/kg/BW of oregano essential oil, and (vi) MOP + TEO treated with 5 mg/kg/BW of Malathion-D10 and 100 mg/kg/BW of thyme essential oil. The results indicated the protective effects of OEO and TEO against MOP-induced weight loss. Additionally, there was a significant improvement in ALT, AST, and ALK-Ph after being treated with OEO and TEO, either alone or after MOP exposure. Also, treatment with OEO and TEO ameliorated these oxidative stress parameters, indicating their antioxidative properties. A histopathological examination of liver tissues showed reduced hepatocellular damage and improved liver architecture in the OEO and TEO, both alone and in combination with MOP, and protective effects were more pronounced in the TEO-treated groups. However, the results indicated that TEO was more effective than OEO in increasing CYP1A2 expression and alleviating MOP-induced toxicity. Specifically, TEO showed higher protein expression and therapeutic action in reducing liver damage. In conclusion, these findings suggest that OEO and TEO may be potent therapeutic agents against MOP toxicity, offering protective effects by enhancing CYP1A2 activity and mitigating organ damage. Such knowledge would be an important step toward developing potentially unique treatment options for natural antitoxins.

Biological properties of vitamin B12.

Vitamin B12, cobalamin, is indispensable for humans owing to its participation in two biochemical reactions: the conversion of l-methylmalonyl coenzyme A to succinyl coenzyme A, and the formation of methionine by methylation of homocysteine. Eukaryotes, encompassing plants, fungi, animals and humans, do not synthesise vitamin B12, in contrast to prokaryotes. Humans must consume it in their diet. The most important sources include meat, milk and dairy products, fish, shellfish and eggs. Due to this, vegetarians are at risk to develop a vitamin B12 deficiency and it is recommended that they consume fortified food. Vitamin B12 behaves differently to most vitamins of the B complex in several aspects, e.g. it is more stable, has a very specific mechanism of absorption and is stored in large amounts in the organism. This review summarises all its biological aspects (including its structure and natural sources as well as its stability in food, pharmacokinetics and physiological function) as well as causes, symptoms, diagnosis (with a summary of analytical methods for its measurement), prevention and treatment of its deficiency, and its pharmacological use and potential toxicity.

Selenium(II)-Nitrogen Exchange (SeNEx) Chemistry: A Good Chemistry Suitable for Nanomole-Scale Parallel Synthesis, DNA-encoded Library Synthesis and Bioconjugation.

The continuous development of click reactions with new connecting linkage is crucial for advancing the frontiers of click chemistry. Selenium-nitrogen exchange (SeNEx) chemistry, a versatile chemistry in click chemistry, represents an all-encompassing term for nucleophilic substitution events that replace nitrogen at an electrophilic selenium(II) center, enabling the flexible and efficient assembly of linkages around a Se(II) core. Several SeNEx chemistries have been developed inspired by the biochemical reaction between Ebselen and cysteine residue, and demonstrated significant potential in on-plate nanomole-scale parallel synthesis, selenium-containing DNA-encoded library (SeDEL) synthesis, as well as peptide and protein bioconjugation. This concept aims to present the origins, advancements, and applications of selenium(II)-nitrogen exchange (SeNEx) chemistry while also outlining the potential directions for future research in this field.

Molecular diagnosis of E.coliO157:H7 Which Isolated from Children with Diarrhea by using Multiplex PCR

A total of 96 stool samples were collected from children with bloody diarrhea from two hospitals in Baghdad. All samples were surveyed and examined for the presence of the Escherichia coli O157:H7 and differentiate it from other Non -Sorbitol Fermenting Escherichia coli (NSF E. coli). The Bacterial isolates were identifed by using morphological diagnostic methods, Samples were cultured on liquid enrichment medium, incubated at 37C? for 24 hrs, and then cultured on Cefixime Tellurite -Sorbitol MacConkey Agar (CT- SMAC). 32 non-sorbitol fermenting bacterial isolates were obtained of which 11 were identified as Escherichia coli by using traditional biochemical tests and API20E diagnostic system without differentiation between serotype O157:H7 and other NSF E. coli isolates . Four special biochemical tests were done for serotype O157:H7 differentiation from other NSF bacteria. Only 3 isolates belonging to the serotype O157:H7 were obtained . Latex agglutination test for O157 and H7 showed that the 3 isolates gave positive results with both tests. The Bacterial isolates were identifed by using Multiplex Polymerase Chain Reaction (MPCR) technology for the presence or absence of 4 genes (Stx1, Stx2, hlyA and eaeA) that encode for main virulence factors to diagnose E. coli O157:H7 isolated By using specific primers in MPCR . The result showed that one E. coli O157:H7 isolates contain all 4 genes , other isolates contain 3 genes: Stx2, hlyA & eaeA.

Assessment of biochemical biomarkers and environmental stress indicators in some freshwater fish

The mechanism by which an organism can adapt to subtle environmental changes is predicated on modifications to biochemical processes within the cellular metabolism in response to such changes. Changes in these processes have the potential to induce alterations in cellular structures and tissue organization, as well as establish a causal link between fluctuations in these parameters and stressors exposure. This investigation's main goal and innovation is to evaluate the environmental stress indicators in the aquatic ecosystem of Lake Qarun. Pesticide residues in freshwater fish should be the primary focus of evaluation of environmental stressor concentrations, since they serve as bioindicators at different times and places on a spatiotemporal scale. A thorough analysis of suggestive biochemical biomarker reactions should also be conducted. The effects of environmental stressors, specifically pesticide contamination in Qarun Lake, have been observed and investigated in relation to two fish species: Solea aejabtiaca and Oreochronis niloticus. The results of a hazard assessment conducted at six sampling sites using spatio-temporal data revealed elevated mean values for the pesticides, persistent organic pollutants (POPs), organochlorines, organophosphates, and pyrethroids that were detected. A multi biomarker approach facilitates a more comprehensive understanding of stress responses induced by exposure to pollutants. As a result, the activities of the biochemical biomarkers CYP-450, GST, GSH, and LDH in the blood and liver of fish samples were found to be notably elevated. The suitability of the identified variables for biomonitoring of aquatic pollution was validated, and the data unveiled variations in sensitivity among species, implying that Nile tilapia could potentially function as a bioindicator with high sensitivity. The findings were correlated with the concentrations of detrimental organochlorines, organophosphorus, and pyrethroids in the muscles and gills. The data indicates that pollutants linked to agricultural wastes, runoff, and municipal effluent may be discharged into the lake ecosystem. Consequently, to safeguard the environment, it is essential to enforce and implement policies, acts, and regulations that already exist. Assessing the effects of additional environmental stressors on aquatic ecosystems is another way in which biomarker screening with an integrative approach improves our comprehension of how toxicants impact various levels of biological organization and is particularly useful in realistic environmental exposure scenarios.

Stable Isotope Tracing Experiments Using LC-MS.

Metabolomics has emerged as a pivotal field in understanding cellular function, particularly in the context of disease. In numerous diseases, including cancer, alterations in metabolism play an essential role in disease progression and drug response. Hence, unraveling the metabolic rewiring is of importance to find novel diagnostic and therapeutic strategies. Isotope tracing is a powerful technique for delving deeper into the metabolic wiring of cells. By tracking an isotopically labeled substrate through biochemical reactions in the cell, this technique provides a dynamic understanding of cellular metabolism. This chapter outlines a robust isotope tracing protocol utilizing high-resolution mass spectrometry coupled to liquid chromatography in cell culture-based models. We cover essential aspects of experimental design and analyses, providing a valuable resource for researchers aiming to employ isotopic tracing.

B-013 Improving the Workflow of Blood and Coagulation Tests in clinical laboratory through the Utilization of Laboratory Automation Track Systems

Abstract Background The Laboratory Automation Track System (TLA) is an automated system designed for executing highly repetitive tasks within a laboratory, commonly applied in biochemical and immunological testing systems. It aims to reduce human errors in specimen handling, enhance the overall process quality, and provide better Turnaround Time (TAT) from specimen collection to result reporting. For the first time in our laboratory, we have integrated the blood and coagulation systems with TLA. Consequently, we seek to evaluate the differences in the testing workflow and reporting efficiency of the blood and coagulation systems after being connected to TLA. Methods Abbott GLP systems Track was the TLA system for connecting with blood and coagulation systems. Two Sysmex XN-9100 fully automatic hematology systems and two Sysmex CS-5100 fully automatic coagulation analyzers were developed for blood and coagulation system, respectively. TUNYEN Laboratory Information System was established for Laboratory information system (LIS). Results Through the analysis of quality indicators and integration with intelligent information systems, improvements have been made, including the reduction of blood collection tube usage, efficient manpower utilization management, tracking of specimen progress and achievement of timely reporting rates.(1)Approximately 2,915 blood collection test tubes are saved every month, with a converted cost of 0.15 USD per tube, and a total saving of 437 USD. And reduce the carbon emissions of 32 kgCO2e, comply with the spirit of ESG sustainable development.(2)The TLA no longer requires staff to collect and deliver specimens as in the past,reducing staff and enhancing work efficiency.One of manpower was saved and new inspection items were developed.(3)Tracking of specimen progress:The laboratory has set up a specimen tracking system to monitor the specimen delivery location and provide timely response to the clinical status.The one-hour TAT for blood and coagulation increased from 82.0% and 76.9% before improvement to 91.8% and 93.3% after improvement respectively. Conclusions The implementation of Laboratory Automation Track Systems (TLA) leads to a reduction in medical errors and specimen sample volume, while also enhancing accuracy and precision, thereby improving the safety of laboratory staff. Although the introduction of TLA may require an initial investment, it brings the benefits of automation, offering ease and efficiency to laboratory processes.

High-affinity antibodies specific to the core region of the tau protein exhibit diagnostic and therapeutic potential for Alzheimer’s disease

BackgroundRecent advances in blood-based biomarker discovery are paving the way for simpler, more accessible diagnostic tools that can detect early signs of Alzheimer’s disease (AD). Recent successes in the development of amyloid-targeting immunotherapy approaches mark an important advancement in providing new options for the treatment of AD. We have developed a set of high-affinity monoclonal antibodies (mAbs) to tau protein that have the potential as tools for diagnosis and treatment of AD.MethodsSheep were immunised with either full-length tau (1-441) or truncated paired helical filament (PHF)-core tau (297–391). A stringent bio-panning and epitope selection strategy, with a particular focus directed to epitopes within the disease-relevant PHF-core tau, was used to identify single-chain antibodies (scAbs). These scAbs were ranked by affinity for each epitope class, with leads converted to high-affinity mAbs. These antibodies and their potential utility were assessed by their performance in tau immunoassays, as well as their ability to prevent tau aggregation and propagation. Further characterisation of these antibodies was performed by immunohistochemical staining of brain sections and immuno-gold electronmicroscopy of isolated PHFs.ResultsOur work resulted in a set of high-affinity antibodies reacting with multiple epitopes spanning the entire tau protein molecule. The tau antibodies directed against the core tau unit of the PHF inhibited pathological aggregation and seeding using several biochemical and cell assay systems. Through staining of brain sections and PHFs, the panel of antibodies revealed which tau epitopes were available, truncated, or occluded. In addition, highly sensitive immunoassays were developed with the ability to distinguish between and quantify various tau fragments.ConclusionThis article introduces an alternative immunodiagnostic approach based on the concept of a “tauosome” – the diverse set of tau fragments present within biological fluids. The development of an antibody panel that can distinguish a range of different tau fragments provides the basis for a novel approach to potential diagnosis and monitoring of disease progression. Our results further support the notion that tau immunotherapy targeting the PHF-core needs to combine appropriate selection of both the target epitope and antibody affinity to optimise therapeutic potential.

Digital model of biochemical reactions in lactic acid bacterial fermentation of simple glucose and biowaste substrates

As concerns about the environmental impacts of biowaste disposal increase, lactic acid bacterial fermentation is becoming increasingly popular. Current academic research is aimed at the process optimization by developing digital bioreactors. The primary focus is to develop a digital model mimicking the biochemical reactions. In the light of this, this paper intended to build a digital model of biochemical reactions during the fermentation process of both glucose and biowaste substrates, including white pasta and organic municipal waste. For this purpose, near-infrared (NIR) and mid-infrared (MIR) spectroscopy techniques were used to collect spectral information during the fermentation process. Next, the samples were analyzed by High Pressure Liquid Chromatography (HPLC) to measure their glucose, fructose, arabinose, xylose, disaccharide, lactic acid, and acetic acid contents. The results showed that learning algorithms trained on MIR spectra accurately estimated the biochemical reactions for both glucose and biowaste substrates. For the glucose substrate, the results showed R-squared of 0.97 and RMSE of 4.69 g/L for glucose, and R-squared of 0.98 and RMSE of 2.74 g/L for lactic acid. In the case of biowaste substrate, estimations included glucose (R-squared = 0.97, RMSE = 4.69 g/L), fructose (R-squared = 0.88, RMSE = 1.47 g/L), arabinose (R-squared = 0.98, RMSE = 0.55 g/L), xylose (R-squared = 0.93, RMSE = 1.11 g/L), disaccharide (R-squared = 0.90, RMSE = 0.55 g/L), total sugar (R-squared = 0.98, RMSE = 3.79 g/L), lactic acid (R-squared = 0.98, RMSE = 2.74 g/L), and acetic acid (R-squared = 0.97, RMSE = 0.36 g/L). Regarding NIR spectral data, the predictive models were accurate when the substrate was glucose, however, they failed to accurately estimate the chemical reactions in the case of biowaste substrate. The findings of this study can be used to fulfill the requirements for a continuous fermentation process with the objective of maximizing lactic acid production.

Two-way role of iron-carbon in biochemical reactions: microelectrolysis and enhanced activity of aerobic granular sludge for efficient refractory wastewater treatment

Industrial wastewater contained a large amount of refractory organics, and single treatment processes had limitations. This study investigated the mechanism of refractory organics removal using iron-carbon built-in coupled activated sludge (ICAS) and explored the role and function of iron-carbon (IC) within the ICAS system. The aerobic granular sludge (AGS) cultivated with IC exhibited a loose surface and a tight interior structure. Iron in the AGS concentrated near the outer layer to form a crust, which protected the inner microorganisms. IC promoted EPS secretion and regulated the abundance of positive and negative signaling molecules to maintain AGS stability. Experiments using quinoline as a model refractory organic showed that both physical adsorption by IC and biological adsorption by sludge rapidly fixed a large amount of pollutants, providing a buffer capacity for the system. The iron mineral crust on the AGS surface enhanced quinoline adsorption. Hydroxylation was the first step in quinoline degradation, with IC upregulating the genes iorA/B, qorB, and wrbA involved in this process, and the relative abundances of quinoline-degrading bacteria. Both pyridine ring opening and benzene ring cleavage occurred in the single IC system, and the microelectrolysis process produced •OH and [H], which made degradation pathway for quinoline through IC more complex than microbial degradation. Although the IC-mediated pathway accounted for only a small part of overall quinoline removal in the ICAS system, the ICAS system not only preserved the microelectrolysis process but also enhanced microbial metabolic activity. This work provided insights into the synergistic removal of pollutants and maintenance of AGS stability by the ICAS process, ensuring efficient treatment of refractory organic wastewater.

Identifying the major metabolic potentials of microbial-driven carbon, nitrogen and sulfur cycling on stone cultural heritage worldwide

Microbial activities and biochemical reactions are responsible for the biodeterioration of stone cultural heritage, but information on microbial metabolic potentials remains elusive. Here we profiled microbial community signatures and its functional traits on stone cultural heritage from different climate zones globally using sequencing datasets available publicly. Bacterial community on stone cultural heritage shows a significant separation between BSk (cold semi-arid climate) and Cfb (temperate oceanic climate) with Aw (tropical savanna climate) as a transition region. Importantly, the ubiquity of ammonia oxidizers and nitrite oxidizers on stone cultural heritage under different climates supports the active production and accumulation of nitrates while ammonia/ammonium can be supplied by dinitrogen fixation and dissimilatory nitrate reduction to ammonium (DNRA), together with the hydrolysis of urea, arginine, formamide and cyanate. Sulfate accumulation on stone cultural heritage is mainly resulted from the microbial-driven transformation of organosulfur and thiosulfate, with little dissimilatory reduction of sulfate. Pseudorhodoplanes was identified and reported in elemental sulfur turnover for the first time. Notably, carbon sequestration via the reductive tricarboxylic acid (rTCA) cycle and an incomplete 3-hydroxypropionate/4-hydroxybutynate (HP/HB) cycle other than the Calvin Benson-Bassham (CBB) cycle is also significant on stone cultural heritage under relatively humid climate. These results advance our understanding of microbial metabolic potentials and their genetical partitioning patterns on stone cultural heritage of different climate zones globally.

Absolute concentration robustness: Algebra and geometry

Motivated by the question of how biological systems maintain homeostasis in changing environments, Shinar and Feinberg introduced in 2010 the concept of absolute concentration robustness (ACR). A biochemical system exhibits ACR in some species if the steady-state value of that species does not depend on initial conditions. Thus, a system with ACR can maintain a constant level of one species even as the initial condition changes. Despite a great deal of interest in ACR in recent years, the following basic question remains open: How can we determine quickly whether a given biochemical system has ACR? Although various approaches to this problem have been proposed, we show that they are incomplete. Accordingly, we present new methods for deciding ACR, which harness computational algebra. We illustrate our results on several biochemical signaling networks.

Spatial patterns of the Brusselator model with asymmetric Lévy diffusion

Abstract The formation of spatial patterns is an important issue in reaction–diffusion systems. Previous studies have mainly focused on the spatial patterns in reaction–diffusion models equipped with symmetric diffusion (such as normal or fractional Laplace diffusion), namely, assuming that spatial environments of the systems are homogeneous. However, the complexity and heterogeneity of spatial environments of biochemical reactions in vivo can lead to asymmetric diffusion of reactants. Naturally, there arises an open question of how the asymmetric diffusion affects dynamical behaviors of biochemical reaction systems. To answer this, we build a general asymmetric Lévy diffusion model based on the theory of a continuous time random walk. In addition, we investigate the two-species Brusselator model with asymmetric Lévy diffusion, and obtain a general condition for the formation of Turing and wave patterns. More interestingly, we find that even though the Brusselator model with symmetric diffusion cannot produce steady spatial patterns for some parameters, the asymmetry of Lévy diffusion for this model can produce wave patterns. This is different from the previous result that wave instability requires at least a three-species model. In addition, the asymmetry of Lévy diffusion can significantly affect the amplitude and frequency of the spatial patterns. Our results enrich our knowledge of the mechanisms of pattern formation.

Chaotic mixing coupled electromagnetic heating in a tubular reactor

To address the need for enhanced biochemical reactions and overcome the shortcomings associated with passive and active mixer fabrication, reactor designs must utilize the coupled mixing concept to address the lack of insufficient heat and mass transfer through notable alterations of the physical properties. This study introduces a tubular microwave reactor based on chaotic flow dynamics, attempting to combine the concept of active and passive mixing to stimulate the development of eddy and secondary flows through electromagnetic fields and geometric disturbance enhance heat and mass transfer. Experiments and simulation analysis validate the validity of the prediction model, and reveal the fluid flow, mixing, electromagnetic and thermal characteristics and their synergistic mechanism, and shows the potential to enhance the mass and heat transfer performance at low Reynolds number, which provides a new idea for the design of chemical reactors and biological analysis.

The versatile multi-functional substance NMN: its unique characteristics, metabolic properties, pharmacodynamic effects, clinical trials, and diverse applications.

β-nicotinamide mononucleotide (NMN) is a naturally occurring biologically active nucleotide widely present in organisms and an inherent substance in the human body. As a critical intermediate in synthesizing coenzyme I (NAD+), it widely participates in multiple biochemical reactions in the human body and is closely related to immunity, metabolism, and other factors. In recent years, NMN has rapidly developed and made significant progress in medicine, food, and healthcare. However, there is currently a lack of comprehensive reports on the research progress of NMN, as well as exploration and analysis of the current research achievements and progress of NMN. Therefore, this review is based on retrieving relevant research on NMN from multiple databases at home and abroad, with the retrieval time from database establishment to 20 May 2024. Subsequently, literature search, reading, key information extraction, organization, and summarization were conducted with the aim of providing a comprehensive and in-depth analysis of the characteristics, metabolic pathways, pharmacological effects, progress in human clinical trials, and wide applications of NMN in drug development and food applications. Furthermore, it offers personal insights into NMN's potential future developments and advancements to present the current development state and existing challenges comprehensively. Ultimately, this review aims to provide guidance and serve as a reference for the future application, innovation, and progression of NMN research.

Closing in on human methylation-the versatile family of seven-β-strand (METTL) methyltransferases.

Methylation is a common biochemical reaction, and a number of methyltransferase (MTase) enzymes mediate the various methylation events occurring in living cells. Almost all MTases use the methyl donor S-adenosylmethionine (AdoMet), and, in humans, the largest group of AdoMet-dependent MTases are the so-called seven-β-strand (7BS) MTases. Collectively, the 7BS MTases target a wide range of biomolecules, i.e. nucleic acids and proteins, as well as severalsmall metabolites and signaling molecules. They play essential roles in key processes such as gene regulation, protein synthesis and metabolism, as well as neurotransmitter synthesis and clearance. A decade ago, roughlyhalf of the human 7BS MTases had been characterized experimentally, whereas the remaining ones merely represented hypothetical enzymes predicted from bioinformatics analysis, many of which were denoted METTLs (METhylTransferase-Like). Since then, considerable progress has been made, and the function of>80% of the human 7BS MTases has been uncovered. In this review, I provide an overview of the (estimated) 120 human 7BS MTases, grouping them according to substrate specificities and sequence similarity. I also elaborate on the challenges faced when studying these enzymes and describe recent major advances in the field.

Взаємозв’язок поліморфізму генетико-біохімічних систем з показниками молочної продуктивності сучасних українських порід великої рогатої худоби

DNA typing technologies make it possible to detect alleles and their frequencies in biochemical systems of proteins and enzymes, including specific PCR sequences, restriction enzymes and actually single nucleotide polymorphisms. The development of breed-specific SNPs is necessary for genotyping and mapping of associations with milk productivity traits, which will make it possible to identify the most valuable genotypes for their further use in selection and breeding work. The purpose of the study is to determine the allelic and genotypic frequencies, genetic diversity and polymorphism of hemoglobin (HB), ceruloplasmin (CP), posttransferrin (pTf), amylase (Am-1) and the receptor for vitamin D (calcitriol) (GS) to identify the relationship breed-specific features of their genetic structure. The results of the research revealed the influence of the breed of cows on their genetic structure according to the genetic-biochemical systems pTf, GS, HB, CP and Am-1. A clear influence of the genotype factor at the posttransferrin locus was observed, among the studied breeds of cows, on the manifestation of the content and amount of protein in milk. In addition, a probable effect of ceruloplasmin on the parameters of fat and protein in milk was established, depending on the breed of cows. The assessment of the genetic-biochemical system based on the amylase locus established a clear influence of the genotype on the manifestation of milk yield, milk fat and the amount of protein in milk. Thus, the use of polymorphism of individual loci of proteins and blood enzymes in the conduct of selection and breeding work will not only contribute to the improvement of breeding work, but also to the improvement of dairy cattle in general. Therefore, the perspective of breeding work with Holstein cattle should be the assessment of the diversity of the allelogen according to the polymorphic system of proteins and enzymes, which will allow to identify the best genotypes not only in terms of productivity, but also in genetic terms.

Isolation, selection and identification of lactic bacteria from pickled salt lotus root in Giang Thanh district, Kien Giang province

The study was conducted to isolate, select, and identify the lactic acid bacteria strain with the highest lactic acid fermentation ability from pickled lotus root products in Giang Thanh district, Kien Giang province. From three samples of pickled lotus root collected in Giang Thanh district, 11 lactic acid bacteria strains were isolated and preliminarily identified through colony morphology and some biochemical reactions such as Gram staining and catalase reaction. The results of determining the fermentation type showed that all 11 isolated bacterial strains had a homomorphic fermentation type, the main fermentation product was lactic acid. Of which, strain D15 had the highest lactic acid content, reaching 1.87 mg/mL and had the highest biomass growth ability, with an OD600nm value of 0.947. The 16S rRNA gene sequence of strain D15 was determined with a molecular size of 1469 bp. Comparing the 16S rRNA gene sequence of strain D15 on the gene bank, it has a similarity level of 100% with some lactic acid bacteria strains of the species Lactobacillus plantarum and 99.68% when comparing with the 16S rRNA gene sequence of the standard strain Lactobacillus plantarum. Constructing a phylogenetic tree and evaluating the genetic relationship of strain D15, the standard strain Lactobacillus plantarum, and 9 strains with the highest similarity to strain D15 based on the 16S rRNA gene sequence. The results were classified into 2 large groups with a similarity level of 92%, in which strain D15 is in the same group as the standard strain Lactobacillus plantarum and also has a similarity level of 92%.

Phytochemical and Antioxidant Analysis of Artocarpus heterophyllus Peel Extracts

BACKGROUND: Plants have a diverse variety of bioactive compounds such as tannins, alkaloids, flavonoids, carbohydrates and steroids among many others that can offer significant physiological actions in the human body. Phytochemicals are naturally occurring chemical, biological and active compounds found in plants that are of benefit to human health apart from those that act as macronutrients and micronutrients. A. heterophyllus (Jackfruit) belongs to the family Moraceae. Jackfruit tree is native to India and is popular in several tropical and sub-tropical Countries. OBJECTIVE: The aim of this study is to determine the phytochemical constituents, antioxidant properties and gas chromatography mass spectroscopy (GC-MS) of the fruit peel of A. heterophyllus extracts using three different solvents. METHODS: Artocarpus heterophyllus fruits were collected from University of Ibadan botanical garden, identified at the Forestry Research Institute of Nigeria, Oyo State. The peel was extracted and dried at 35 ℃. Qualitative method and Gas Chromatography Mass Spectroscopy were used to determine the phytochemical and bioactive compounds present in the plant, whereas¬¬¬¬¬¬ 2, 2, Diphenylpicryl-1-hydrazyl (DPPH) was used for the antioxidant analysis. RESULTS: Secondary metabolites such as flavonoids, phenols, alkaloids, glycosides, saponins, tannins, steroids, and Terpenoids were present based on the solvents while anthraquinnes were absent. Flavonoid and phenol were abundant in the three extracts while saponin was moderate. Eight chemical compounds were observed in the plant extracts. Major bioactive components with highest and lowest retention peak were (65.55 %) bis(2- ethyl hexyl) phthalate and (6.06 %) Cis- 13(2- ethylhexyl)phthalate respectively. Antioxidant activity increased with increase in concentration of which acetone extract had the highest DPPH (75.00 %) at 100 μg/ml. CONCLUSION: The fundamental means of the usage of medicinal plants for medicine is due to their bioactive properties which are used as substrates for biochemical and enzymatic reactions. Phytochemical derivatives have antiviral, anti-inflammatory, anti-oxidant, immunomodulatory, anticancer, antimicrobial, antitumor, analgesic and many other properties harbor in them.