Biochemical Parameters In Patients Research Articles

Evaluate the Complete Blood Picture and Some Biochemical Parameters in Patients referred to the dialysis unit in Diyala General Hospital

Research done on 40 patients; 24 males, 20-79 years, and 16 females, 42-67 years; referred to dialysis unit, Baqubah hospital, in November and December, 2021; January and February 2022. Creatinine, Urea and Iron, record high levels. Glucose, calcium, sodium, potassium, and phosphate, were within normal, and low levels of albumin. Means of Total Erythrocyte Counts (TECs), hemoglobin (Hb), Hematocrit% (HCT), and Mean Corpuscular Hemoglobin Concentration (MCHC), showed lowered level. While, Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Red Blood Cell Distribution Width- Standard Deviation (RDW-SD), Mean Red Blood Cell Distribution Width- Coefficient of Variation (RDW-CV), were within normal. Total Leucocyte Counts (TLCs), Neutrophils, Lymphocytes, Eosinophil, Basophils, were within normal, Platelet Counts and related parameter were within normal. Within ranges some values were higher than normal as, potassium, phosphate, (RDW-SD), (RDW-CV), Neutrophils%, Eosinophil%, Lymphocytes, Mean Platelets Volume (MPV). While some other values were lower as sodium, (TRCs), (Hb), (HCT %), (MCV), (MCH), (MCHC), (RDW-SD), (TLCs), Lymphocytes%, platelets, Platelet Distribution Width (PDW), Platelet- Large Cell Ratio (P-LCR). But some values of glucose, and calcium, were high, and some other low. At the same time Monocytes%, Basophils% was within normal range. In conclusion creatinine and urea levels have an important role in diagnosis and follow- up of kidney failure. All patients have high levels of urea, iron and creatinine. But not all have higher level of potassium and glucose. Sodium and albumin, showed decreased level. Blood picture revealed microcytic, hypochromic anemia; as (TECs), (Hb), (HCT %), (MCHC) and (RDW-SD) were of lower values.

Association of hypercalcemia with biochemical parameters in patients with endocrine disorders

Background. Although today primary hyperparathyroidism and cancers are considered the main factors of hypercalcemia (HC), the latter is quite often detected in various endocrinopathies. According to modern data, ionized calcium (Ca2+) is an intracellular mediator in the functioning of enzymes and hormones. All this determines the importance of finding out the features of the associations between HC and biochemical indicators in various endocrine disorders, which was the purpose of this study. Materials and methods. Retrospectively, according to the medical histories of 537 patients with detected HC, who were treated at the endocrinology clinic of the V. Danilevsky Institute for the Endocrine Pathology Problems in 2020–2021, we have analyzed the features of correlations between HC (by the content of Ca2+) and the levels of phosphorus (P), total protein (TP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in various endocrine pathologies. Results. A significant difference between the spectrum and degree of probability of associations was found even within the limits of one type of pathological conditions. In the group with hypothyroidism, there was an inverse correlation of HC with P (p<0.01) and a highly probable correlation with AST (p<0.001). At the same time, a direct association with P (p<0.01) and an inverse correlation with TP (p<0.001), AST (p<0.001) and ALT (p<0.01) was revealed in case of Graves’ disease. The following data were obtained for tumors of endocrine organs: nontoxic multinodular goiter— direct with P (p<0.001) and inverse with TP (p<0.001) and ALT (p<0.01); thyroid cancer— direct correlation with P (p<0.01) and inverse highly probable with TP (p<0.001) and ALT (p<0.001); nodular pathology of the adrenal glands— direct correlation with TP (p<0.01) and inverse with AST (p<0.01) and ALT (p<0.001). In diabetes mellitus, only an inverse correlation with P was found (p<0.01). There was an inverse correlation with P (p<0.01) and TP (p<0.001), and a direct one with ALT (p<0.001) in primary hyperparathyroidism. Conclusions. Metabolic disorders in the presence of hypercalcemia in patients with various endocrine pathologies have their own characteristics. The nature of revealed correlations between ionized calcium and biochemical parameters in terms of various endocrine diseases must be taken into account when developing a therapeutic strategy for patients with hypercalcemia.

Association of Adiponectin With biochemical parameters in patients with chronic kidney diseases on Hemodialysis process in Erbil city

Adiponectin (Adipo.) is a hormone produced by adipocytes that circulate in the blood. Serum Adipo. concentrations are elevated in several chronic kidney disease (CKD) conditions and are a predictor of end-stage renal disease. The association between Adipo. levels and the progression of the disease are not well established. This study aimed to determine if there was a relationship between serum Adipo. levels and inflammatory, metabolic, and CKD progression in Erbil. In a case-control study of 200 participants, 100 with CKDs were on hemodialysis (HD) in Erbil teaching hospital. To collect the necessary information from participants, a study questionnaire was used. Adipo. hormone levels and other metabolic parameters were measured. Total Cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL), fasting blood sugar, C-reactive protein (CRP), glycosylated hemoglobin (HbA1c), creatinine, albumin, and urea were all measured in the blood. 90.0% of the cases were at stage 5 of CKD, the Serum Adipo. levels were higher when compared with a healthy control group (13.26 ±0.96 µg/ml vs 2.17±0.75 µg/ml). In conclusion, our study found A high level of Adipo. in CKD patients can predict inflammation and malnutrition and is considered a biomarker for renal dysfunction.

Study the relationship between Apelin and some biochemical parameters in patients with kidney diseases

The study aimed to determine the effect of the lack of peptide apelin in the pathogenesis of kidney patients and the positive impact when increasing its concentration in the body and protect the body from various diseases. The study included 57 patients of different ages and were compared with the control group, which included 25 samples.Through the study, which was conducted and when measuring the concentration of Apelin show that there is a significant decrease (P≤0.01) at a concentration of Apelin in people who suffer from kidney disease compared with the control group, which was Apelin concentrations with in the normal range, and it has been Measuring range of biochemical variables to find a statistically significant correlation between their concentrations and the concentrations of Apelin and results of the study follows :The concentration of nitrogen compounds and their relationship with Apelin levels: found high significant (P≤0.01) at each concentration of urea, creatinine and uric acid in people who are suffering from various kidney diseases compared with the control group, which was within normal of biochemical variables. And their relationship was a correlation with the concentration of Apelin is an inverse relationship. The concentration of proteins in the blood serum concentrations and their relationship Apelin: found a significant increase (P≤0.01) in the concentration of each of the total protein and globulin in people suffering from various kidney disease compared with the control group, which was within normal concentrations of the protein that resides in the body, While the results did not show any significant differences in the concentration of albumin between patients and control group. and their relationship with apelin concentration is an inverse.

Comparison of Therapeutic Feed (Different recipes) during Stabilization Phase for the management of Children with Severe Acute Malnutrition (SAM)

Objective: To compare the feeding of sugar based F75, Cooked rice based F75 and Enhanced cooked rice based modified F75 during stabilization phase for the management of children with SAM (Severe Acute Malnutrition) aged 6-60 months presented with diarrhea. Study Design: An open-label randomized controlled trial. Place and Duration: Stabilization center, Children’s Hospital and Institute of Child Health Multan from 1st January 2018 to 30th November 2019. Methodology: The total 270 children, (who were under 5 years of age and admitted to the stabilization center for complicated Severe Acute Malnutrition (SAM) during study duration) were divided into three groups of 90 patients each. Group A was given Standard sugar-based F75, Group B was given Cooked Rice based F75 and Group C was given Enhanced cooked rice based modified F75. Clinical and biochemical parameters of patients were analyzed at day 1, day 3 and day 7 after admission. Results: The age of our patients was 16.7 ± 12.2 months with male to female ratio of 1.2:1. The mid upper arm circumference (MUAC) of cases was 10.07±0.86 cm. More than half of the children had diarrhea at admission. During the initial stabilization phase (day 1 to day 7), number of patients with complains of diarrhea increased from 57.78% to 65.54% in group A while it reduced from 52.22% to 15.54% in group B and from 62.16% to 7.77% in group C. Conclusion: Modified rice base therapeutic feeding is more effective for treatment of SAM with diarrhea as compared to standard sugar based F75 formula. Keywords: Children, Diarrhoea, Severe Acute Malnutrition, Stabilization Phase, Carbohydrate Malabsorption, Diarrhea, F75 Rice Based Therapeutic Feed How to Cite This: Khan S, Iqbal I, Ali I, Batool N, Abbas A, Arshad R. Comparison of Therapeutic feed (different recipes) during Stabilization Phase for the management of Children with Severe Acute Malnutrition (SAM). Isra Med J. 2022; 14(3): 108-112. DOI: https://doi.org/10.55282/imj.oa1140

Assessment of some hematological and biochemical parameters in patients with renal failure in Al-najaf province

Chronic renal failure is a condition in which the kidneys gradually lose their ability to function because of the gradual loss of their parenchyma. This study aimed to detect some hematological and biochemical parameters in patients with chronic kidney disease (CKD) attending a hemodialysis center. A cross-sectional study included a review of (89) cases of chronic kidney disease at Al-Hakeem Hospital's dialysis center in Najaf from September 7th to October 16th, 2021. The collected data were analyzed using Office Excel and SPSS-23. Variables were analyzed by frequencies, proportions, and percentages. The current study discovered that males had longer sequences than females, and that patients in rural areas had longer sequences than those in urban areas. In the current study, blood Sug, B. Urea, S. Crea, K, Na, Cl, Ca+2, Alb, and Tpro levels did not differ significantly between males and females with renal failure, whereas HB and TCA levels did. At last, we recommend people conduct an early medical examination for kidney disease, especially those who suffer from symptoms such as low urine output, swelling of the legs, fatigue, tachycardia, and others, to obtain early treatment.

Efekat zdravstveno vaspitne intervencije u formi savetovanja na promene antropometrijskih i biohemijskih parametara obolelih od dijabetes melitusa tip 2

Introduction: Diabetes is a significant public health problem. In Serbia, 12% of the population suffers dominantly from type 2 diabetes, the development of which is associated with an inadequate diet and lack of physical activity. The treatment of patients involves the application of diet therapy in combination with medical treatment. The aim of this paper is to evaluate the differences in anthropometric and biochemical parameters in patients with type 2 diabetes after the counseling work. Respondents and methods: The research was conducted as a retrospective evaluation study through the analysis of medical records of users of the Nutrition Counseling Service of the Public Health Institute of Subotica. The sample included 34 patients with type 2 diabetes, mostly women (71%). Results: The average body weight of the patients was 87.6 kg, waist circumference 105 cm, the average glycemima was 7.14 mmol/l, and the blood pressure level was 140/84 mmHg. After counseling, at the first control measurement 2-4 months after the first examination, the patients lost an average of 3 kg, the waist circumference decreased by an average of 2 cm, and the percentage of fat in the body decreased by 1.6%. There was a decrease in glycemia by 0.6 mmol/l and systolic blood pressure by 5 mmHg. Although the second control examination was attended by a much smaller number of patients, based on the results, it was observed that the average values of the observed parameters were significantly lower compared to the initial values (body mass decreased by 1 kg, waist circumference by 4 cm, glycemia level by an additional 1 mmol/l). Conclusion: Based on the results of the research, it can be concluded that after the counseling work, there was a significant improvement in the nutritional status and glycemia in patients with type 2 diabetes.

Changes in the level of zinc and copper and some biochemical parameters in patients with chronic kidney failure

Background: Chronic kidney failure (CRF) is characterized by a progressive loss of functional status over weeks or months, which may result in one of the illness's recognized consequences, such as cardiovascular disease, chest pain, or anemia. CRF has been generally understood as a worldwide public health problem and a big factor to death and morbidity during the previous decade. Methods: Determination of zinc and copper, urea, creatinine, total protein, globulin, alanine aminotransferase (ALT), albumin, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in (CRF) Iraqi patients. This study included (40) patients and (38) healthy subjects as control. Samples were collected from January to April (2022). The biochemical parameters were determined using spectrophotometrically, but zinc and copper were determined by atomic absorption spectrophotometer. Results: Copper, urea, creatinine, ALP, AST, ALT, and LDH were significantly higher in CRF patients, whereas zinc, total protein, albumin, and globulin were lower in CRF patients as compared with control. The research also concerned study the relation between sex (male and female) and biochemical parameters which showed significant differences in comparison male of patients with males of control, yet globulin showed nonsignificant differences. While patients and control females showed nonsignificant differences. Conclusions: LDH in CRF patients might be used as a biomarker to detect renal dysfunction in dialysis patients at an early point, and blood serum Zn deficit in CRF. Furthermore, a relationship among Zn and albumin amounts was discovered. As a result, Zn and copper in CRF, particularly hypoalbuminemia, must be frequently evaluated and adjusted.

The Effect of Vitamin D on IFN-gama and Biochemical Parameters in Patients with Metabolic Syndrome

The Effect of Vitamin D on IFN-gama and Biochemical Parameters in Patients with Metabolic Syndrome

Inflammatory prognostic index predicts new-onset atrial fibrillation and mortality after on-pump coronary artery bypass grafting.

This study aimed to analyze its predictive role in incipient postoperative atrial fibrillation by constructing an inflammatory prognostic index based on hematological and biochemical parameters in patients undergoing elective isolated coronary artery surgery accompanied by cardiopulmonary bypass. The data of 343 patients who underwent coronary bypass surgery between May 2021 and July 2022 were evaluated. Multivariate logistic regression and recipient study characteristic curve analyses were studied by comparing the patients' hematological indices and basic clinical features between the two groups. Logistic regression analysis showed that age (p<0.001), hypertension (p=0.01), and inflammatory prognostic index (p<0.001) were independent predictors of new-onset postoperative atrial fibrillation. To predict the development of postoperative atrial fibrillation, a cutoff value of 0.25 (77.8% sensitivity and 69.3% specificity) was determined for inflammatory prognostic index in the receiver-operating characteristic curve analysis (area under curve=0.798, 95% confidence interval 0.752-0.840). Inflammatory prognostic index can be a noninvasive, easily available marker for predicting new-onset atrial fibrillation after coronary artery bypass surgery.

Study the Effect of chemical treatments on the physiological and biochemical parameters of patients with cancerous diseases in Thi-Qar Governorate

Study the Effect of chemical treatments on the physiological and biochemical parameters of patients with cancerous diseases in Thi-Qar Governorate

БІОХІМІЧНІ ПОКАЗНИКИ КРОВІ У ХВОРИХ НА РОЗСІЯНИЙ СКЛЕРОЗ, ЯКІ ПЕРЕНЕСЛИ ІНФЕКЦІЮ SARS-CoV-2

B a c k g r o u n d. Currently, multiple sclerosis (MS) is an important medical and social problem. There are already more than 2.8 million people with MS worldwide, and due to the lack of effective methods of treatment and prevention, the number of patients is increasing constantly. Therefore, the search for new ways and approaches aimed towards stabilizing the patient's condition is a priority task of modern neurobiology. It is also important to identify triggers that can lead to accelerating the rate of disease progression. Since MS is an immune-mediated disorder, we hypothesized that concomitant diseases of infectious etiology, via the activation of an inflammatory reactions cascade, can lead to a worsening of the MS patient's condition. However, the final answer to the question of whether acute viral infections affect the condition of patients with MS has not been obtained yet. In the context of the epidemiological situation that has developed in recent years, due to the spread of the SARS-CoV-2 coronavirus infection, it is interesting to study the possible negative consequences of COVID-19 in people suffering from MS. The aim of the study was to analyze the general biochemical status of patients with MS who had or did not have a confirmed history of COVID-19. M e t h o d s. A total of 271 participants were enrolled in this study, including 97 MS patients and 30 healthy volunteers who matched for age and sex. Patients with MS were divided into two groups: group I (n= 56; 36 women and 20 men, average age: 41.3±5.5, disease duration: 4.4±1.5 years) – patients with MS who did not have confirmed history of COVID-19 and group II (n= 41; 27 women and 14 men, average age: 40.2±5.6, disease duration: 4.6±1.3 years) – patients with MS, who had a laboratory-verified diagnosis of COVID-19 in the past 4-7 months. Blood serum samples of patients and healthy donors were obtained from the University Clinic of O.O. Bogomolets National Medical University, Kyiv, Ukraine. All patients were diagnosed with MS according to the 2017 McDonald criteria. R e s u l t s. It was established that both groups of MS patients had similar changes in blood biochemical parameters compared to the healthy indicators: a decrease in creatinine and uric acid concentrations; decrease in the levels of particular lipid fractions, namely VLDL and TAG; decrease in the activities of AP and LDH; increase in C-reactive protein concentration. The only significant differences between groups I and II were noted due to multidirectional changes in serum ferritin concentration and an increase in the level of amylase activity in the blood serum of patients with MS in post- COVID period. C o n c l u s i o n s. The absence of statistically significant differences between the blood biochemical parameters in patients with MS who had or did not have confirmed history of COVID-19 indicates the absence of a relationship between SARS-CoV-2 infection and changes of particular studied parameters. Among the results obtained, the multidirectional changes in ferritin concentration in MS patients before and after COVID-19 could be of great interest to further research.

Noninvasive Assessment of Liver Fibrosis by Magnetic Resonance Elastography in Patients with Rheumatic Disease on Long-Term Methotrexate Treatment

Background: Long-term methotrexate (MTX) use is associated with hepatic fibrosis in patients with rheumatic diseases. Liver biopsy (invasive conventional diagnostic procedure for hepatic fibrosis) is associated with sampling errors and procedural risks. Magnetic resonance elastography (MRE) is a novel, reliable, and noninvasive imaging technique with excellent diagnostic accuracy for staging hepatic fibrosis and cirrhosis. Till date, studies are scarce on its use in staging MTX-induced liver fibrosis and cirrhosis. The aim of this study is to assess the usefulness of MRE in detecting and quantifying liver fibrosis and to compare these with biochemical parameters in patients with rheumatic diseases on long-term MTX therapy. Methods: Patients with different rheumatic diseases, aged ≥18 years and on MTX treatment for >5 years were included in the study. Their medical records were reviewed, and data regarding demographics, diagnosis, disease duration, MTX dosage and duration were collected. On the day of MRE examination, series of biochemical parameters were conducted in patients. Predefined cutoff MRE values were used for staging liver fibrosis. Results: A total of 48 subjects diagnosed with different rheumatic diseases on long-term MTX were recruited in the study, and majority of them were female (n = 41). The mean age and body mass index of the patients were 53.56 ± 8.36 years and 28.08 ± 4.43, respectively. Around 37.5% (n = 18) of the patients had abnormal aspartate transaminase (AST)-to-platelet count ratio index (APRI) score. The mean MRE value of the study group was 2.83 ± 0.90 kPa. Around 45.83% (n = 22) of the patients had normal liver stiffness values (<2.5) whereas stages F0 (2.5–2.9 kPa), F1 (2.9–3.5 kPa), F2 (3.5–4.0 kPa), F3 (4.0–5.0 kPa), and F4 (>5.0 kPa) were observed in 12.5% (n = 6), 18.75% (n = 9), 10.42% (n = 5), 10.42% (n = 5), and 2.08% (n = 1) of the patients, respectively. MRE values did not correlate with disease duration and cumulative dose of MTX. However, a positive correlation was observed between MRE and liver biochemical parameters (AST: Alanine transaminase [ALT] ratio, ALT, platelet count, APRI, albumin, and mean liver size; P > 0.05). Conclusion: Considering the risk for complications with liver biopsy, MRE provides a reliable, highly accurate, noninvasive assessment of hepatic fibrosis in patients with rheumatic diseases receiving long-term MTX therapy.

Association between inflammatory cytokines and other Biochemical parameters in patients after 48 hrs of first myocardial infarction (MI)

Myocardial infarction (MI) is a term used to describe a heart attack that occurs when blood flow to the heart muscle reduced, and plaque builds up in the lining of the coronary arteries, resulting in insufficient oxygen supply. The study was conducted on a number of participants, including 100 cases and 70 controls, who were matched for gender and age. The blood indicators in question were examined using standard laboratory techniques and equipment. The study aimed to study the relationship between inflammatory cytokines, lipid profiles, and transaminase enzyme in patients 48 hours after myocardial infarction. The results indicated that there are differences between cases and controls for inflammatory cytokines (IL-8, IL-12, and IFN-Αα). Moreover, total cholesterol (TC), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were decreased in cases in comparison with controls, while triglyceride (TG) was high in patients after MI. However, patients' aspartate transaminase (AST) levels were meaningfully higher than in controls.

Association of C‑peptide with immunological and biochemical parameters in patients with type 1 diabetes mellitus

Type 1 diabetes mellitus (T1DM) is an autoimmune metabolic disorder characterized by the destruction of pancreatic β‑cells due to the autoimmune reactions of autoreactive T‑cells and autoantibodies. The present study aimed to investigate the association between the levels of connecting peptide (C‑peptide), and several biochemical and immunological markers in patients with T1DM treated with insulin. For this purpose, a total of 5 ml venous blood was collected from 44 patients with T1DM and 28 healthy volunteers. Relevant clinical examinations were carried out. The contents of C‑peptide, IL‑4 and IL‑5 were determined using the corresponding ELISA kits. The normal ranges for liver and renal function tests were set for both patients and controls. The results revealed that the mean concentrations of hydrogen peroxide (H₂O₂) was higher in patients with T1DM compared with healthy controls. However, the difference was not statistically significant. In addition, the levels of IL‑5 were decreased in patients with T1DM compared with healthy individuals. No statistically significant correlation was observed between the levels of C‑peptide and cytokines with the exception of IL‑4 in healthy volunteers, which were positively correlated with those of C‑peptide. Overall, the results demonstrated that the low C‑peptide levels in patients with T1DM were not associated with the measured parameters. However, patients with T1DM could still be at high risk of developing pathological complications due to the enhanced levels of glucose and H₂O₂, and the decreased levels of IL‑5.

Study of Some Immunological Physiological and Biochemical Parameters in Patients with Type 1 Diabetes Mellitus in Kirkuk City

This study was designed in order to estimate some immunological (IL-6), physiological (Resistin) and biochemical (Malondialdehyde and Glutathione) parameters of some patients with type 1 diabetes mellitus in Kirkuk city. The study involved (60) blood samples were divided into 2 groups: group one: normal healthy individuals as control group, group two: patients with type 1 diabetes mellitus. The results of this study found significant increase in pro-inflammatory cytokine IL-6 concentrations at levels (P≤ 0.05) of second group in comparison with control group ; and there are significant increases in resistin concentrations at levels (P≤ 0.05) of second group in comparison with control group; and there are significant decreases in glutathione (GSH) concentrations at levels (P≤ 0.05) of second group in comparison with control group, and there are significant increases in malondialdehyde (MDA) concentrations at levels (P≤ 0.05) of second group in comparison with control group.

Thyroid function analysis and other biochemical parameters in patients with moderate and severe COVID-19

Background and objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease-19 (COVID-19). The effects of COVID-19 on the thyroid axis remain uncertain. This study aimed to determine the occurrence of thyroid dysfunction, as previously demonstrated for SARS-CoV-1 infection, and to analyze electrolytes (sodium, potassium, and chloride) to determine the severity of the disease. Methods: Blood samples were taken from patients who were admitted to Erbil hospitals from July to October 2021. Infection was detected by polymerase chain reaction (PCR). The 306 cases were labeled as moderate or severe. The samples were assessed for doing C-reactive protein (CRP), as a marker of systemic inflammation, thyroid-stimulating hormone (TSH), triiodothyronine (T3) and thyroxine (T4), and (sodium, potassium, and chloride) electrolytes. Results: Patients comprised of 157 (51.3%) males and 149 (48.7%) females. The result revealed that 274 (89.5%), 278 (90.8%), 292 (95.4%) of patients had normal TSH, T3, T4 level respectively, 22 (7.2%), 23 (7.5%), 9 (2.9%) of patients had low TSH, T3, T4 level respectively, and 10 (3.3%), 5 (1.6%), 5 (1.6%), had high TSH, T3, T4 level respectively. There was no significant difference between moderate and severe cases in alterations of TSH, T3, T4 level. There was a significant difference between moderate and severe cases in sodium, potassium, and chloride electrolyte abnormality. Conclusion: The occurrence of thyroid dysfunction in moderate and severe cases. The severity of the COVID-19 infection is associated with abnormalities in sodium, calcium, and chloride electrolytes.

Effect of Helicobacter pylori eradication on metabolic profile: an international, multicenter, case-control study

As a gram-negative and microaerophilic bacterium, Helicobacter pylori (HP)is the main cause of chronic gastritis. Therefore, considering the high prevalence of HP infection worldwide, as well as the increasing prevalence of metabolic disorders, the present study aimed to investigate the relationship between HP infection eradication and metabolic profile. This prospective case-control study was performed on patients with HP infection whom referred to 7 medical centers in 3 countries (Iran, Egypt, and Vietnam) in 2020-2021. The metabolic profile of all of the participants evaluated before starting of treatment for HP eradication and 3 months after the treatment. Then changes of metabolic profile compared between those with successful HP eradication (group A) and subjects who failed to eradicate (group B). Overall, 199 patients, including 93 male (46.7%) with the mean age of 44.5 years (18-93 years) included. Based on response to treatment, the participants allocate into group A (those who respond to HP eradication): 164 cases (82.42%); or group B as those who failed to achieve eradication (35 cases, 17.58%). Racially 86.9% of participants were Caucasian and 89% diagnosed as non-ulcer dyspepsia (NUD). The most prevalent comorbidity include hypertension (11.5%) and hyperlipidemia (10%) which were more prevalent in group B (P = 0.002).Three months after therapy, average weight of participants among those who achieved eradication (group A) decreased from 73.1 to 71.4kg (P = 0.01), but in comparison with group B, was non-significant (P = 0.171). The BMI of patients before and after treatment did not show any significant differences. The biochemical parameters of patients before and after treatment were not significantly different regardless of treatment success (P > 0.05). The levels of total cholesterol and VLDL cholesterol after treatment were not significantly different from baseline values in two groups. HDL and LDL cholesterol levels before and after treatment in the resistant group were significantly higher than the responding group. Average serum TG level decreased significantly after treatment in the group A (P < 0.0001), in contrast to the resistant group (P = 0.356).The liver transaminases (AST and ALT) before and after treatment were not significantly different between the two groups (P > 0.05). The results of logistic regression showed that the eradication of infection has no significant affect any of the metabolic profile parameters. HP infection treatment in individuals without significant metabolic disorders does not affect metabolic parameters up to 3 months after eradication. HP eradication among subjects with several comorbidities mandates eradication protocol intensification to avoid treatment failure.

A simple covert hepatic encephalopathy screening model based on blood biochemical parameters in patients with cirrhosis.

Covert hepatic encephalopathy (CHE) adversely affects clinical outcomes in patients with liver cirrhosis, although its diagnosis is difficult. This study aimed to establish a simple CHE screening model based on blood-related biochemical parameters. This retrospective study enrolled 439 patients who were assessed for CHE using a neuropsychiatric test between January 2011 and June 2019. A simple CHE (sCHE) score was calculated with hypoalbuminemia (≤ 3.5 g/dL) and hyperammonemia (≥ 80 μg/dL) as 1 point each. The association between sCHE score and CHE or overt hepatic encephalopathy (OHE) was assessed using logistic regression and Fine-Gray competing risk regression models. Of 381 eligible patients, 79 (21%) were diagnosed with CHE. The distribution of sCHE scores was 48% with 0 point, 33% with 1 point, and 19% with 2 points. Patients with sCHE score ≥ 1 point had a higher prevalence of CHE than those with sCHE score of 0 (27% vs. 14%, P = 0.002). A cut-off value of 1 point showed high discriminative ability for identifying CHE, with a sensitivity of 0.67, specificity of 0.56, positive predictive value of 0.27, and negative predictive value of 0.86. During the median follow-up period of 2.2 years, 58 (15%) patients developed OHE. Multivariate analysis showed that sCHE score ≥ 1 (sub-distribution hazard ratio [SHR], 2.69; 95% confidence interval [CI], 1.41-5.15) and CHE (SHR, 2.17; 95% CI, 1.26-3.73) independently predicted OHE. The sCHE score is a useful screening model for identifying patients with CHE and for predicting OHE occurrence.

Clinical characteristics and the influence of rs1800470 in patients with Camurati-Engelmann disease.

Camurati-Engelmann disease (CED) is a sclerosing bone dysplasia caused by transforming growth factor β1 (TGFB1) gene variants. We aim to summarize the clinical characteristics and the efficacy of glucocorticoids in 14 individuals with CED, and explore the correlation between the phenotype and the SNP of rs1800470 (c.29C>T). Clinical, biochemical, radiological, and therapeutic data were collected from 14 patients. DNA was extracted for TGFB1 variants detection by Sanger sequencing. The median onset and record age were 3.0 and 16.1 years, respectively. All patients manifested bone pain and decreased subcutaneous fat tissue. Inflammatory markers increased in over 60% of patients, and the median erythrocyte sedimentation rate (ESR) was 1.40 (0.50~3.67) of the upper limit of normal (ULN), and the median high sensitivity C reactive protein (hsCRP) was 1.71 (0.48~12.56) of ULN. There was a positive correlation between ESR and hsCRP (rs=0.806, p=0.003). Both ESR and hsCRP were negatively correlated with the levels of hemoglobin (HGB), calcium, and creatinine, but positively correlated with the level of alkaline phosphatase. Four known variants of TGFB1 were identified, including p.Tyr171Cys, p.Arg218Cys, p.Arg218His, and p.Cys225Arg. Moreover, 35.7% and 28.6% of them carried the heterozygous and homozygous SNP of c.29C>T, called C/T and T/T groups, respectively, but 35.7% of them were without c.29C>T (C/C group). The onset age, anthropometric data, percentages of different clinical manifestations, and biochemical parameters were comparable among the three groups. But there were increasing trends in levels of HGB and calcium and decreasing trends in ESR and hsCRP among C/C, C/T, and T/T groups in turn. Glucocorticoid improves the two inflammatory markers among CED patients. The phenotype of CED is highly heterogeneous. There is no clear genotype-phenotype correlation, but it seems to have better trends of biochemical parameters in patients with CED carrying the T allele of rs1800470.