Polycystic ovary syndrome during childbearing age is consider as one of the commonest endocrine disease in reproductive age females, with a prevalence of 4% to 15%. The clinical expression of PCOS is variable, but mostly includes anovulation or oligo-ovulation, clinical or biochemical hyperandrogenism and ovarian polycystic picture in U\S. Objective: To determine the levels and prevalence of thyroid antibodies in euthyroid women has PCOS.Patients and method: A case control study carried in the Infertility clinic of the Babylon Teaching Hospital for maternity and children in Al-Hila city, Babylon governorate, /Iraq in the period from October 1, 2018 to the end of Nov. 2019. Results: the current study found a highly statistically significant association between the studied group among the BMI (P 0.05). Eighty percent of the patients (PCOS) group, and 20% in control group presented with family history with thyroid disease. Anti-TG were negative in (85.72%) of the PCOS group patients, 8.57% of the patients with borderline positive and only (5.71%) were positive (>110 IU/ml). TSH level in PCOS group (2.3 ± 0.5) were increase with significant association than that in control group (2.1 ± 0.8), highly significant increase were found in Anti-TPO, Anti-TG, free testosterone and LH in PCOS group than that in control group (P<0.001).Conclusion: There is increased risk of thyroid disease with increase in thyroid Autoimmunity Markers in PCOS patients.