Biochar Application Research Articles

The long-term effect of biochar application to Vitis vinifera L. reduces fibrous and pioneer root production and increases their turnover rate in the upper soil layers

Fibrous and pioneer roots are essential in the uptake and transport of water and nutrients from the soil. Their dynamic may be influenced by the changing of soil physicochemical properties due to the addition of biochar, which, in turn, has been shown to improve plant growth and productivity in the short term. However, the long-term effects of biochar application on root dynamics are still widely unknown. In this study, we aimed to investigate the long-term effects of biochar application on grapevine fibrous and pioneer root dynamics and morphological traits in relation to soil characteristics. To this aim, grapevine plants amended in 2009 and 2010 respectively with one and two doses of biochar, were analyzed in their fibrous and pioneer root production and turnover rate, standing biomass, length, and specific root length, over two growing seasons. Our findings demonstrate that in the long term, biochar application significantly increased soil pH, nutrient availability, and water-holding capacity causing a decrease in the production of fibrous and pioneer roots which is reflected in a reduction of the root web characterized though by a higher turnover rate. Furthermore, we observed that these root morpho-dynamical changes were of higher magnitude in the upper soil layers (0-20 cm) and, at least in the long term, with no significant difference between the two doses. These results suggest that in the long term, biochar can be a powerful tool for improving soil quality, which in turn lowers carbon-cost investment toward the root production and maintenance of a reduced root web that might be directed into grapevine growth and productivity. Such effects shed some light on the root plastic and functional adaptation to modified soil conditions facilitated by the long-term application of biochar, which can be used for implementing adaptive agricultural practices to face the current climate change in a frame of sustainable agricultural policies.

Biochar addition enhances remediation efficiency and rapeseed yield in copper-contaminated soil

IntroductionSoil contamination with copper (Cu) threatens ecological security and human health. Rapeseed demonstrates potential in remediating copper-contaminated soil, and biochar-assisted phytoremediation is increasingly being employed to improve remediation efficiency. However, the combined application of them has not been thoroughly studied in terms of the synergistic effects and the mechanisms of their interaction.MethodsIn this regard, this study conducted a pot experiment to evaluate biochar-assisted remediation under Cu-contaminated soil with varying biochar application rates; Furthermore, the plant physiological mechanism and soil physicochemical properties involved in the biocharrapeseed system was explored.ResultsOur results showed that the exchangeable pool of copper in soil decreased by 10.0% and 12.3% with adding 5% biochar (BC1) and 10% biochar (BC2) relative to control (BC0), respectively, prior to rapeseed cultivation. The rapeseed cultivation for one season further reclaimed 4.9%, 9.0%, and 13.6% of the available copper in this soil by root extraction under the BC0, BC1, and BC2 treatments, respectively. The overall copper concentration in plants decreased by 23.7% under BC2 and 13.3% under BC1 compared to BC0. However, the plant’s dry biomass at BC1 and BC2 treatments increased by 1.7-fold and 2.7-fold relative to BC0, which offset the negative impact of the decreased copper concentration on phytoremediation. Physiological analysis showed adding 10% biochar decreased the MDA content by 36% in the leaf and 49% in the root, compared to BC0. The transmission electron microscopy for cell wall ultrastructure in root tips showed that biochar addition in Cu-contaminated soil increased the mechanical strength of the celL wall, explicitly increasing the thickness of the secondary cell wall. Further cell wall components analysis revealed a remarkable increment of the pectin content in BC2 relative to BC0, increased by 56% in the leaf and 99% in the root, respectively. Additionally, 10% biochar application led to a roughly 2-fold increase in seed yield via ameliorating the soil physicochemical properties and increasing the rapeseed growth.DiscussionThese findings offer insights into synergistic rapeseed-biochar use for Cu-contaminated soil remediation.

Effects of maize residue and biochar applications on soil δ13C and organic carbon sources in a subtropical paddy rice ecosystem

AbstractThis study investigates the utility of plant δ¹3C natural labeling in predicting the impacts of environmental shifts on carbon cycling within ecosystems, particularly focusing on paddy fields treated with maize (Zea mays L.) residues and biochar. Specifically, it examines how soil δ¹3C and the sources of soil organic carbon (SOC), respond in paddy fields (which cultivate C3 plants like rice) when amended with maize residues, maize biochar, and silica‐enriched biochar (derived from C4 plants). Conducted in the Fuzhou paddy fields, the experiment included control groups and treatment groups with maize residue (4 t ha⁻¹), maize biochar (4 t ha⁻¹), and silicon‐modified maize biochar (4 t ha⁻¹) during both the early and late rice growth periods. The results indicate that all soil treatments increased soil δ¹3C. The application of maize residues notably affected the δ¹3C of the upper soil profile (0–15 cm) differently from the deeper layers (15–30 cm), and it increased soil organic C more than biochar or silicon‐modified maize biochar. Soil available P (AP) and pH emerged as significant factors linking δ¹3C, influencing rice yield through changes in soil physicochemical properties. Unlike maize residues, which reduced rice yields, applications of biochar and silicon‐modified maize biochar increased rice yields. The latter, which was particularly effective in lowering SOC decomposition rates and addressing rice's silica needs, emerged as the preferred option. The study highlights maize biochar and silicon‐modified maize biochar as sustainable alternatives to maize residues for rice cultivation, enhancing soil fertility, carbon pool stability, and yields.

The Effects of Acid-Modified Biochar and Biomass Power Plant Ash on the Physiochemical Properties and Bacterial Community Structure of Sandy Alkaline Soils in the Ancient Region of the Yellow River

The application of biochar can effectively enhance soil organic matter (SOM) and improve soil structure. Biomass power plant ash (BPPA) is also rich in essential nutrients for plants, with similar carbon content. Considering production cost and agricultural waste recycling, it is beneficial to apply BPPA to improve soil fertility and quality. However, it remains unclear whether its ameliorative effects surpass those of biochar in alkaline soils. In the study, we set up seven pot experiments of faba beans in sandy alkaline soils from the ancient region of the Yellow River, including the controls (CK), different amounts of acid-modified BPPA (A1, A2, A3), and the same amounts of acid-modified biochar (B1, B2, B3), to compare their effects on soil physiochemical properties and bacterial community structure. The results indicate that the application of both biochar and BPPA can improve soil physiochemical properties. At the same dosage, the biochar application outperformed BPPA treatment in terms of soil physical properties such as bulk density (BD), maximum water-holding capacity (FC), and soil capillary porosity (SP2). Conversely, BPPA treatment displayed advantages in chemical properties such as readily oxidizable organic carbon (ROOC), total nitrogen (TN), alkaline nitrogen (AN), available phosphorus (AP), available potassium (AK), and electrical conductivity (EC). All the treatments enhanced the richness and diversity of bacterial communities, increasing the relative abundance of eutrophic groups such as Bacteroidota and Firmicutes while decreasing that of oligotrophic groups like Actinobacteriota. BPPA also increased the relative abundance of Proteobacteria, while the opposite was observed for biochar. Correlation analysis showed that the environmental factors such as soil pH, EC, TN, AK, SOM, and SP2 emerged as primary factors influencing the bacterial community structure of alkaline soils, significantly affecting their diversity and abundance. Among them, SP2 and SOM were the dominant physical and chemical factors, respectively. Overall, the application of both acid-modified BPPA and biochar can enhance the physiochemical properties of sandy alkaline soils, while the application of BPPA is superior for improving soil nutrient content and enhancing bacterial community structure. The study explores the potential mechanisms through which the application of acid-modified BPPA affects soil characteristics and microbial features, providing new insight into developing optimizing fertilization strategies for enhancing soil quality in the ancient region of the Yellow River.

Post-Extractivism and Bioeconomy: An Experimental Analysis of Combustion and Pyrolysis Processes as Alternatives to Add Value to Agro-Residues (Coffee Husks) Generated in Farmer Cooperatives of the Ecuadorian Amazon

A post-extractivist development model for communities in the Amazon that is not based on non-renewable resource extraction demands the study and demonstration, in the field, of alternative economic activities that add value to currently generated residual biomass. Following the principles of bioeconomy, this study presents an experimental analysis of a retort burner and a pilot-scale auger-type pyrolysis reactor used to convert coffee husks generated in a collection and post-harvesting center of a farmer’s cooperative into thermal energy and biochar, respectively. This study shows that coffee husks, whether used as feedstock for combustion or pyrolysis processes, can supply the thermal energy required by the post-harvesting processes. The combustion or pyrolysis of coffee husks avoids its accumulation and decomposition while replacing fossil fuels used in post-harvesting operations, reducing costs and making farmers independent of fossil fuel subsidies. Unlike combustion (11,029.4 mg/Nm3), the CO concentration in the flue gas during the pyrolysis process was 458.3 mg/Nm3, which is below the eco-design standard of 500 mg/Nm3. According to the European Biochar Certificate, carbon content (67.4 wt%) and H/Corg, O/Corg (0.6 and 0.1, respectively) are within the typical values of biochars used for soil amendment and carbon sequestration. Nonetheless, the concentration of polycyclic aromatic hydrocarbons must be assessed to fully regard this material as biochar. Finally, further studies are required to assess the ability of cooperatives to generate and trade carbon credits linked with the application of biochar in their cropping systems.

Impact of Poultry Manure-Derived Biochar and Bio-Fertilizer Application to Boost Production of Black Cumin Plants (Nigella sativa L.) Grown on Sandy Loam Soil

Biochar derived from poultry manure increases nutrient availability and promotes plant growth. This study investigated the effect of biochar with mycorrhizal and/or plant growth-promoting rhizobacteria on soil fertility, chemical properties, oil, and seed yield of Black Cumin (Nigella sativa L.) plants. A split-plot design with three replicates was employed, with biochar derived from poultry litter (BC) applied at rates of 0, 5, and 10 t ha−1, with beneficial microbes such as arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) affecting the growth of Black Cumin plants, and some soil properties, such as pH, electrical conductivity (EC), soil organic matter (SOM) and fertility index (FI), showing significant differences (p ≤ 0.05) among biochar and/or bio-fertilizer treatments. All biochar treatments with or without bio-fertilizers significantly increased pH, EC, OM and FI in comparison to the control treatment. The results demonstrated that applying biochar at the highest rate (10 t ha−1) increased fresh and dry capsule weights by 94.51% and 63.34%, respectively, compared to the control treatment (C). These values were significantly increased by 53.05 and 18.37%, compared to untreated plants when combined with AMF and PGPR. Furthermore, when biochar was applied in conjunction with both AMF and PGPR, fresh and dry capsule weights saw significant increases of 208.84% and 91.18%, respectively, compared to the untreated control treatment. The interaction between biochar, AMF, and PGPR significantly improved plant growth, yield, soil properties, and the fixed and volatile oil content of Black Cumin. These findings suggest that the combined application of biochar, AMF, and PGPR enhances nutrient availability and uptake, leading to improved growth and higher yields in Black Cumin plants, resulting in increased yield production.

Pinewood biochar antistatic polymer composites: an investigation of electrical, mechanical, rheological and thermal properties

ABSTRACT This study investigates the practical applications of biochar as an innovative and sustainable conductive filler in composite materials. Utilizing the conventional melt-blending method, we prepared conductive pinewood biochar-filled poly(lactic acid)/poly(butylene adipate-co-terephthalate) (PLA/PBAT) composites and systematically examined their electrical, mechanical, rheological and thermal properties. Our results reveal a significant enhancement of electrical and mechanical properties upon the inclusion of conductive pinewood biochar in the PLA/PBAT matrix. With the addition of 10 wt% of conductive pinewood biochar, the average surface resistivity of the composites decreased by three orders of magnitude, measuring 4.86 × 10 10 Ω / sq , which meets the antistatic requirements for polymer composites. Additionally, the average tensile strength of the biochar filler composites improved by 53% with an addition of just 1 wt% of biochar.

Biochar mitigation potential in Norway estimated by IPCC Tier 1 and Tier 2 methods

Biochar is a recalcitrant carbon-rich solid produced by pyrolysis of organic residues, and its application to soil is considered a promising approach to mitigate climate change, as biochar resists decomposition to readily contributes to soil carbon (C) sequestration. The IPCC provides a basis for future national-scale accounting of the changes in soil C stocks following biochar application to cropland soils. The IPCC Tier 1 approach for biochar is based on fixed emission factors to estimate biochar C sequestration. In contrast, the Tier 2 approach allows countries to use local emission factors and climate data to calculate the contribution of biochar to soil C sequestration. Accurate accounting of biochar C sequestration is essential for ensuring the credibility of C offsetting projects, as well as providing incentives for implementing biochar in C credit schemes, calling for comparative analyses of the different biochar Tier approaches. Here we retrieved biochar samples from local producers and measured their H/Corg to estimate the persistence of biochar in Norwegian croplands post application. Various feedstocks were considered, including forest residues, woody wastes, manure, sludge, and straw. For all biochar samples, the 100-year stable C fraction was calculated at ≥ 0.945, thus exceeding the default Tier 1 value (0.8). Biochar sourced from woody- and forestry residues had a Corg content above the default Tier 1 value (0.77). Based on this and data about national feedstock supplies, we compared the theoretical potential of biochar soil C sequestration to mitigate climate change in Norway, using the IPCC Tier 1 and Tier 2 approaches. Biochar C sequestration in soil was calculated at 0.79 Tg CO2-eq yr−1 and 0.92 to 0.96 Tg CO2-eq yr−1, respectively for the Tier 1 and Tier 2 approaches, thus, underlining that the choice of IPCC Tier approach can have a large impact on the estimated mitigation potential of biochar.

Optimization of water and nitrogen management in wheat cultivation affected by biochar application − Insights into resource utilization and economic benefits

In the context of global climate change and natural resource scarcity, agricultural production is facing multiple challenges in improving crop yields and optimizing natural resource use. It is of great practical significance to optimize agricultural practices to achieve sustainable agricultural development. In this study, a two-year winter wheat field experiment was conducted in the Guanzhong Plain, Shaanxi, China, from 2020 to 2022 to assess the effects of biochar, irrigation, and N fertilizer rates on the yield, water-nitrogen use efficiency, and economic benefits of winter wheat. Specifically, biochar was applied to winter wheat at 30 t ha−1 in combination with two irrigation application rates, including regular irrigation (I100; actual evapotranspiration) and deficit irrigation (I80; 0.8 actual evapotranspiration), and three different N fertilizer rates at 210 (NH; conventional applied N rate by local farmers), 160 (NM; moderate N rate), and 110 kg ha−1 (NL; low N rate). The control groups in this study consisted of experimental plots under the NM and N0 (no N) without biochar application. The aboveground dry matter mass (ADM), yield, and net ecosystem economic budget (NEEB) of winter wheat showed increasing trends with increasing N application rates without significant differences between the NH and NM treatments. On the other hand, the water use efficiency (WUE), agronomic N fertilizer use efficiency (aNUE), and N fertilizer recovery efficiency (NRE) showed increasing-decreasing trends with increasing N fertilizer rates, reaching the highest values under the NM treatment scenario. The biochar addition significantly increased the winter wheat yield and WUE (P<0.05). On the other hand, the I80 treatment resulted in higher WUE and irrigation water use efficiency (IWUE) than those under I100 by 5.63 and 13.52 %, respectively. TOPSIS results indicated that the combined I80B1NM treatment was the optimal winter wheat management practice, maintaining high productivity while improving resource use efficiency and economic benefits. The results of the present study provide an important scientific basis and guidance for ensuring efficient and high-quality agricultural products in the Guanzhong Plain and other regions in China with similar climatic characteristics.

A quantitative review of the effects of biochar application on the reduction of Cu concentration in plant: a meta-analysis.

Contamination and toxicity of copper (Cu) in soils are global issues, particularly in regions where Cu-based fungicides are utilized. Elevated Cu concentrations can lead to soil contamination and pose significant risks to the ecosystem, including plant life, wildlife, and human health. The application of biochar has been proposed as a viable strategy to mitigate Cu accumulation in plants. However, there is no quantitative and data-based consensus on the impact of biochar on plant Cu accumulation. In this meta-analysis, 624 data records from 65 published literature were collected and the effects of various factors, including biochar properties, experimental conditions, and soil properties on Cu accumulation in plants, were examined through meta-subgroup analysis and meta-regression models. The results obtained indicate a significant dose-dependent effect of biochar in decreasing Cu concentration in plants by an average of 23.45%. Soils with acidic pH values and medium textures were more conducive for biochar to mitigate Cu accumulation in plant tissues. In addition, manure biochar and green waste biochar were found to be more successful in decreasing Cu concentrations in plants compared to other biochar types. Biochar types with pyrolysis temperatures of > 600°C and pH values of ≥ 10 resulted in greater decreases in plant Cu concentration. Regarding biochar application, biochar minimum range of 1% in potting experiments and 20 t/ha in field experiments have been recommended to effectively decrease Cu concentration in plants. Overall, these findings provide valuable insights into Cu transfer mitigation through food chain to human bodies and for policymakers to take preventive measures.

Application of straw-derived biochar: a sustainable approach to improve soil quality and crop yield and reduce N2O emissions in paddy soil.

The burning of agricultural straw is a pressing environmental issue, and identifying effective strategies for the rational utilization of straw resources is decisive for achieving sustainable development. Owing to its high carbon content and exceptional stability, straw biochar produced via pyrolysis has emerged as a key focus in multidisciplinary research. However, the efficacy of biochar in mitigating nitrous oxide (N2O) emissions from paddy soils is not consistent. A 2-year field experiment was conducted and investigated the impact of biochar derived from two feedstocks (rice straw and wheat straw, pyrolyzed at 450°C) on N2O emissions, global warming potential (GWP), greenhouse gas intensity (GHGI), nitrogen use efficiency (NUE), crop yield, and soil quality. The static chamber technique was used for collecting N2O gas samples, and concentrations were analyzed through gas chromatography methods. The treatment combinations included BR0 (control), BR1 (NPK at the recommended dose, 120:60:40kgha-1), BR2 (wheat straw biochar, 5 t ha-1), and BR3 (rice straw biochar, 5 t ha-1). The results exhibited that cumulative N2O emissions from BR2 and BR3 treatments decreased by 10.55% and 13.75% respectively, compared to BR1. Lower GWP and GHGI were observed under both biochar treatments compared with BR1. The highest rice grain yield (3.48Mgha-1) and NUE (76.72%) were recorded from BR3, which also exhibitedthe lowest yield-scaled N2O emission. We observed positive correlations between soil nitrate, ammonia and water-filled pore spaces, while NUE showed negative correlations with N2O emissions. Significant (p < 0.05) improvements in soil quality were also detected in both the biochar treated plots, indicated by increased soil pH, water holding capacity, porosity, and nutrient contents. Overall, the results suggest that applying biochar at a rate of 5 t ha-1 in paddy soil is a viable nutrient management strategy with the potential to reduce reliance on inorganic fertilizers, mitigate N2O emissions, and contribute to sustainable food production.

Effects of Combined Biochar and Chemical Fertilizer Application on Soil Fertility and Properties: A Two-Year Pot Experiment

A two-year pot experiment was conducted to investigate the effects of the combined application of biochar and chemical fertilizer on soil quality and vegetable growth by adding different proportions of chemical fertilizer and biochar to the soil in 2022 and no fertilizer in 2023. It was concluded that the combined treatment improved the soil’s properties. After two consecutive years of planting vegetables, the improvement of soil properties was the most significant with the 1.5 g biochar + 80% chemical fertilizer optimal fertilizer application (BCF6) treatment. In comparison to the control (CK), soil pH, electrical conductivity, and dissolved organic carbon increased by 0.59 units, 166.6%, and 282.6%, respectively. Soil fertility also improved significantly, indicating that the combined treatments resulted in the slow release of nutrients to enhance the effectiveness of the fertilizers. Co-application significantly increased the yield of the edible parts of Chinese cabbage and improved its quality. The most significant effects of vitamin C content and soluble protein were observed in Chinese cabbage under BCF6 treatment, which were 3.33 and 1.42 times more than the CK, respectively. Utilizing biochar as a partial substitute for chemical fertilizers can improve soil structure and fertility over the long term while reducing the reliance on chemical fertilizers, ultimately providing sustained economic and ecological benefits for agricultural production.

Iron-modified biochars and their aging reduce soil cadmium mobility and inhibit rice cadmium uptake by promoting soil iron redox cycling

Iron (Fe) modified biochar has been widely used for cadmium (Cd) contaminated soil remediation. However, the accompanying anions introduced during the modification process potentially affect the behavior of Cd in soil. In this study, we investigated the distinct Cd immobilization mechanisms by Fe2(SO4)3 modified biochar (FSBC) and Fe(NO3)3 modified biochar (FNBC) in a two-year pot experiment. Results showed that both FSBC and FNBC significantly reduced Cd concentrations in rice grains by 23%–42% and 30%–37% compared to pristine biochar (BC). Specifically, NFBC promoted the formation of amorphous Fe oxides by enhancing the NO3−-reducing Fe(II) oxidation process, which significantly increased Fe/Mn oxide-bound Cd and decreased soil CaCl2-extractable Cd. For FSBC, the introduction of SO42− significantly promoted the formation of Fe plaques by enhancing the Fe(III) reduction process, which blocked the Cd transfer from the soil to the rice roots. More importantly, after two years of biochar application, an organo-mineral complex layer is formed on the biochar surface, which immobilized a large amount of Cd. The Cd immobilization on the surface of aged biochar could be due to the fixation by the secondary Fe oxides within the organo-mineral layer and the complexation by the surface functional groups. The result of laser ablation inductively coupled plasma mass spectrometry showed that the Cd content on aged FNBC and FSBC was 5.9 and 2.6 times higher than on aged BC. This might be attributed to the Fe-modified biochar's higher electron exchange capability (EEC), which promoted the development of organo-mineral complexes. Notably, the EEC of biochar was maintained during its aging process, which may keep the biochar surface active and facilitate continual Cd immobilization. This study revealed the complex mechanisms of soil Cd immobilization with Fe-modified biochar, providing new insights into sustainable biochar environmental remediation.

Differential responses of two local and commercial guar cultivars for nutrient uptake and yield components under drought and biochar application

Drought is one of the abiotic stresses that can reduce crop yields. It has a major impact on crop yield reduction. For crops under stress, organic modifiers such as biochar can be useful. Guar (Cyamopsis tetragonoloba L.), an annual legume from the Fabaceae Family, is highly adaptable to arid and semi-arid regions, with many applications in various industries. Field experiments were carried out in a randomized complete block design with three replications using a split-split plots arrangement. The aim was to evaluate the influence of irrigation levels (Ir1 = 10, Ir2 = 14, and Ir3 = 17 days irrigation cycle) and biochar (B1 = 0, B2 = 5, and B3 = 10 tons ha−1) application on physiological traits [(chlorophyll a and b, chlorophyll index (SPAD), relative leaf water content (RWC), electrolyte leakage (EL), canopy temperature, leaf area, water use efficiency (WUE)], morphological parameters (length and diameter of the stem, pod length, fresh weight of root and plant, root length), yield components (seed yield, number of branch plant−1, number of clusters plant−1, pod plant−1, seed pod−1, seed plant−1, 1000-seed weight, and gum contents), and leaf nutrient uptake (Ca, Mg, P, Na, and K) of two commercial and local cultivars (cv1 = RGC-936 and cv2 = Saravan) of the guar plant. It was observed that the Ir3 irrigation treatment produced the highest seed yield (1921.8 kg ha−1) in terms of water stress. However, the maximum pod plant−1 (75.5), seed plant−1 (454.2), seed yield (1871.1 kg ha−1), leaf area (861.8 mm2), SPAD (92.2), Mg (49.8 mg g−1), Na (43.3 mg g−1) and P (0.49 mg g−1) were observed in RGC-936. The results also revealed that biochar was more effective than cultivars in terms of morphological traits. While yield and yield components were affected by cultivar, irrigation at different levels also had a significant effect on functional traits, physiology, and morphology. The addition of biochar appeared to have a positive effect on water stress alleviation and guar growth and leaf nutrient uptake. According to Pearson’s correlation analysis, plant weight and length, root weight and length, stem diameter, seed pod−1, branches plant−1, and 1000-seed weight are moderately correlated with seed yield, while pod plant−1 and seed plant−1 are strongly associated with seed yield. On the other hand, the pod length, branches plant−1, and gum content showed a positive but not significant relationship.

Rice husk biochar is more effective in blocking the cadmium and lead accumulation in two Brassica vegetables grown on a contaminated field than sugarcane bagasse biochar.

Heavy metal-contaminated soil has a great impact on yield reduction of vegetable crops and soil microbial community destruction. Biochar-derived waste biomass is one of the most commonly applied soil conditioners in heavy metal-contaminated soil. Different heavy metal-contaminated soil added with suitable biochars represent an intriguing way of the safe production of crops. This study investigated the effects of two types of biochar [rice husk biochar (RHB) and sugarcane bagasse biochar (SBB)] on Cd and Pb accumulation in Shanghaiqing (SHQ, a variety of Brassica campestris L.) and Fengyou 737 (FY, a variety of Brassica napus), as well as on the soil microbial community, through a field experiment. RHB and SBB were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and Brunauer-Emmet-Teller method. The results showed that RHB and SBB displayed the higher pH, cation exchange capacity and pore properties, and the addition of RHB and SBB enhanced soil pH and rhizosphere microorganisms promoting vegetables yield. RHB treatments were more effective than SBB in reducing upward transfer of Cd and Pb, blocking the accumulation of Cd and Pb in the edible parts of SHQ and FY, and decreasing soil Cd and Pb bioavailability. Additionally, RHB and SBB changed the composition of the rhizosphere soil microbial community. The application of biochar promoted the growth of ecologically beneficial bacteria (Nitrospira, Opitutus, and Gemmatimonas) and fungi (Mortierella and Holtermanniella), whereas reducing the enrichment of plant pathogenic fungi (Alternaria, Stagonosporopsis, Lectera, and Periconia) in rhizosphere soil. Our findings demonstrated that the application of RHB significantly reduces Cd and Pb accumulation in the edible parts by decreasing the soil Cd and Pb bioavailability and altering the rhizosphere microbial community composition in two Brassica vegetables grown on Cd/Pb-contaminated soils. Thus, the application of two biochar, especially RHB is a feasible strategy for the safe production of vegetable crops in Cd/Pb co-contaminated soils.

Decoupled fungal and bacterial functional responses to biochar amendment drive rhizosphere priming effect on soil organic carbon mineralization

The application of biochar to soil is widely recognized as a promising strategy for enhancing the accumulation and stability of soil organic carbon (SOC), which is crucial in mitigating climate change. However, the influence of interactions between plants and biochar on soil microbial communities and their involvement in SOC mineralization and stability remains unclear. This understanding is essential for optimizing carbon (C) sequestration in systems involving plants, soil, and biochar. In this study, employing a 13C natural abundance approach, we investigated the effect of biochar on the maize rhizosphere priming effect (RPE) in paddy soil. We also examined alterations in microbial communities and functional genes related to C degradation and fixation. Over the 99 days of maize growth, biochar application increased RPE and total SOC while decreasing dissolved organic C. It also elevated soil pH, resulting in shifts in fungal and bacterial community structure, favoring oligotrophic species. Fungal and bacterial assemblies were dominated by deterministic and stochastic processes, respectively. While the abundance of fungal guilds varied irregularly, bacterial guilds were uniformly enriched under biochar-plant interactions. Functional traits such as ecoenzymatic activities, bacterial guilds, and functional genes predominantly affected RPE under biochar application. Bacterial functional genes associated with C degradation and fixation were concurrently enhanced with biochar application. Our results indicate that interactions between plants and biochar can enhance native SOC mineralization and accumulation in a short timeframe by modulating functional traits of soil microorganisms, particularly the bacterial community involved in C degradation and fixation.Graphical

Co-application effects of rice husk biochar and different types of nitrogen fertilizers on rice yield, yield components, and nitrogen use efficiency

The Nitrogen (N) recovery index in rice paddy soils has never been exceeded by more than 30%, regardless of using proven fertilizer management strategies and amounting to significant N being out of rice plant use. Biochar application and the newly released N biofertilizers (singly or in combination) increase the N use efficiency by increasing the soil nutrient retention capacity. Therefore, a two-year pot experiment was conducted to explore the effects of the co-application of rice husk biochar and nitrogen fertilizers on rice yield, yield components, and nitrogen use efficiency indexes. The applied treatments were rice husk biochar at three levels (0, 20, and 40 tha−1) and N fertilizers at three levels (0, chemical (Urea), and N-biofertilizer). The most effective combination treatments (200 kg N ha−1 in source of urea plus 40 tha−1 rice husk biochar) significantly (p ≤ 0.01) increased the grain yield (2.38 times), straw and biological yield (2.83 and 2.62 times, respectively), N content of soil N (59%), nitrogen uptake efficiency (2.92 times), and nitrogen internal efficiency (59%), whereas, nitrogen recovery efficiency decreased by about 52%. Moreover, the maximum increase in N content of grain and harvest index was recorded at combination application of 20 tha−1 biochar plus urea by about 48 and 15%, respectively. It can be concluded that the co-application of rice husk biochar and N fertilizers has substantial advantages over their single applications in terms of N fertilizer recovery, N uptake. Thus, it might be an effective way to enhance rice yield.

Biochar affects organic carbon composition and stability in highly acidic tea plantation soil

Biochar amendments are effective in stabilizing soil aggregates and improving soil organic carbon (SOC) content. However, the effects of biochar on highly acidic soil and their relation with soil SOC stability remain understudied. The study aimed to investigate the impact of biochar on changes of aggregate distribution and SOC stability in a highly acidic tea plantation soils over an eight-year period. Soil samples were collected from plots with varying biochar application amounts (0, 2.5 t ha−1, 5 t ha−1, 10 t ha−1, 20 t ha−1 and 40 t ha−1). The content of SOC, iron bound organic carbon (OC-Fe), particulate organic carbon (POC), mineral-associated organic carbon (MAOC) and the functional group composition of SOC was analyzed. The results indicated that in the biochar application treatments, the value of soil pH, SOC, POC and MAOC contents were increased from 3.92 to 4.28, 6.68%–187.02%, 8.31%–66.78% and 13.07%–236.47% respectively, compared with CK, while the content of macro-aggregate (particle size>0.25 mm) and soil aggregates mean weight diameter (MWD) significantly increased with higher biochar application amounts. But dissolved organic carbon (DOC) and OC-Fe content exhibited downward trend, decreased from 2.43% to 6.97% and 4.18%–19.91%. Furthermore, aromatic-C levels increased, with increased biochar application amounts. The integration of biochar not only bolstered soil aggregate stability but also amplified the presence of aromatic-C, thereby enhancing the resilience of organic carbon in highly acidic tea garden soil (BC40 > BC20 > BC5>BC2.5 > BC10 > CK), with increases ranging from 6% to 47%. The principal component analysis and structural equation modeling identified soil pH, TN, SOC, POC, MAOC, R > 0.25 and MWD as key factors of soil organic carbon stability. These findings provide crucial insights into the mechanism underlying biochar's efficiency in fortifying organic carbon stability, particularly in the context of highly acidic soil.

The Combination of Biochar and Phosphorus-Containing Materials Can Effectively Enhance the Remediation Capacity of Amaranth on Cadmium-Contaminated Soil and Improve the Structure of Microbial Communities

Cadmium (Cd) pollution in soil has become a huge problem for agricultural production in China and even the world. Passivation and phytoremediation are two important remediation technologies for Cd pollution. In this study, the cadmium-contaminated and phosphorus-poor farmland soil around a mining area in Huainan was taken as the research object, and the remediation effect of biochar and phosphorus-containing materials on soil cadmium pollution was discussed. The results showed that the combined application of biochar and phosphorus-containing materials significantly reduced the pH of non-rhizosphere soil and rhizosphere soil, and increased the content of soil dissolved organic carbon (DOC). The combined application of biochar and phosphorus-containing materials significantly reduced soil pore water Cd and soil available Cd. In addition, both a single application of biochar and synergistic application of biochar and phosphorus-containing materials significantly increased the biomass of aboveground and underground parts of amaranth and soil urease and catalase activities. Phosphorus application reduced the bioavailability of Cd in soil. With the increase in phosphorus application, the content of available Cd in soil decreased significantly, and there was a certain negative correlation between Cd content and phosphorus content in plants. The abundance of beneficial microorganisms such as Ochrobactrum, Anaerolinea, Achromobacter, and Cellvibrio in soil was significantly increased after the synergistic application of biochar and phosphorus-containing materials.

Novel nanocomposite and biochar insights to boost rice growth and alleviation of Cd toxicity

Cadmium (Cd) is an unessential and pervasive contaminant in agricultural soil, eventually affecting the food and instigating health issues. The implication of nanocomposites in agriculture attained significant attention to drive food security. Nanocomposites possess exceptional characteristics to stun the challenges of chemical fertilizers that can enhance plant yield and better nutrient bioavailability. Similarly, biochar has the ability to immobilize Cd in soil by reducing mobility and bioavailability. Rice husk biochar is produced at high temperature pyrolysis under anoxic conditions and a stable carbon-rich material is formed. To strive against this issue, rice plants were subjected to Cd (15, 20 mg kg− 1) stress and treated with alone/combined Ca + Mg (25 mg L− 1) nanocomposite and rice husk biochar. In our study, growth and yield traits showed the nurturing influence of Ca + Mg nanocomposite and biochar to improve rice defence mechanism by reducing Cd stress. Growth parameters root length 28%, shoot length 34%, root fresh weight 19%, shoot fresh weight 16%, root dry weight 9%, shoot dry weight 8%, number of tillers 32%, number of grains 20%, and spike length 17% were improved with combined application of Ca + Mg and biochar, with Cd (20 mg kg− 1), rivalled to alone biochar. Combined Ca + Mg and biochar application increased the SPAD 23%, total chlorophyll 26%, a 19%, b 18%, and carotenoids 15%, with Cd (20 mg kg− 1), rivalled to alone biochar. MDA 15%, H2O2 13%, and EL 10% were significantly regulated in shoots with combined Ca + Mg and biochar application with Cd (20 mg kg− 1) compared to alone biochar. POD 22%, SOD 17%, APX 18%, and CAT 9% were increased in shoots with combined Ca + Mg and biochar application with Cd (20 mg kg− 1) compared to alone biochar. Cd uptake in roots 13%, shoots 14%, and grains 21% were minimized under Cd (20 mg kg− 1) with combined Ca + Mg and B. pumilus application, compared to alone biochar. Subsequently, combined Ca + Mg and biochar application is a sustainable solution to boost crop production under Cd stress.