Biocellulose Production Research Articles

Optimization of Bacterial Cellulose Production by Komagataeibacter rhaeticus K23

The use of bacterial cellulose (BC), having high purity, a high degree of crystallinity, water-holding capacity, tensile strength and adaptability on a broad scale is limited because of the low yield. In this study, the optimal conditions for bio-cellulose production by Komagataeibacter rhaeticus K23 were investigated. Optimal values for temperature, pH, inoculum concentration and incubation time were determined via Taguchi design. The maximum BC production, 9.1 ± 0.66 g·L−1 (dry weight), was obtained from 32 °C, pH 5.5, 8 log CFU·mL−1 and 14 days of incubation. The inoculum concentration was the most significant factor affecting BC yield. A value of 8 log CFU·mL−1 and 14 days of incubation led to significantly higher levels of BC yield than other concentrations (8.5, 9, 9.5, 10 and 10.5 log CFU·mL−1) (p < 0.002) and days (15, 16, 17, 21 and 28) (p < 0.001). The studied features, namely absorption peaks (Fourier transform infrared spectroscopy), pattern and the crystallinity index (X-ray diffraction analysis) of the BC obtained in this study were all in parallel with the characteristics of cellulose I. The study demonstrates that optimized parameters were effective in producing BC with high water-holding capacity, tensile strength, elongation and Young’s modulus (mechanical tests) by K. rhaeticus K23.

First report of biocellulose production by an indigenous yeast,Pichia kudriavzeviiUSM-YBP2

AbstractHerein, we describe the first report ofPichiastrain producing biocellulose (BC). This yeast was isolated from rotten pineapple and was identified asPichia kudriavzeviiUSM-YBP2 using 18S rDNA analysis. The formation of pellicle was characterized using a scanning electron microscope (SEM). Based on SEM analysis, pellicles are presented as an interwoven network of fibers. Next, to produce high BC, optimization was conducted using different carbon and nitrogen sources, carbon and nitrogen concentrations, inoculum size, and cultivation condition. The optimized parameters for maximum BC production were as follows: glucose 8.0% (w/v), peptone 2.0% (w/v), yeast extract 0.5% (w/v), disodium phosphate 0.270% (w/v), citric acid 0.115% (w/v), ethanol 0.5% (v/v), and inoculum size 10% (v/v). The production yield is 12.24 ± 0.43 g/L and the productivity of 0.128 g/L/h. Then, BC was further treated using sulfuric acid to destructuring to bionanocellulose (BNC) and was characterized using the transmission electron microscope (TEM), attenuated total reflection-Fourier transform infrared spectroscopy, and X-ray diffraction (XRD) analysis. After acid hydrolysis treatment, BNC showed a spherical shape as in TEM. Interestingly, BNC characteristic band displayed high similarity with cellulose from other bacterial species. Moreover, BNC showed high crystallinity index (∼82%) as estimated using XRD. Thus, our findings substantiated that strain USM-YBP2 showed remarkable BNC production with unique BNC property that can be further explored in various applications.

Health benefits of Kombucha: drink and its biocellulose production

Kombucha (tea and biocelluose) has been used worldwide due to its high nutritional, functional, and economic potential. This fermented tea has been used in folk medicine to treat several pathological conditions and its biocellulose in the industrial sector. In this context, this paper presents a scientific literature review on the main phytochemicals of Kombucha and respective biological activities to assess their potential uses. The tea has presented a wide range of bioactive compounds such as amino acids, anions, flavonoids, minerals, polyphenols, vitamins, and microorganisms. Moreover, its biocellulose is rich in fibers. These compounds contribute to various biological responses such as antioxidant, hepatoprotective, antitumoral, antidiabetic, and antihypercholesterolemic effects. In this sense, both the tea and its biocellulose are promising for human use. Besides, Kombucha presents itself as a drink option for vegetarians and/or those seeking healthier diets, as its biocellulose can bring metabolic benefits. Our review demonstrates that both can be used as functional foods and/or sources of bioactive compounds for food and industrial applications.

Optimization and Characterization of Biocellulose Production from Bacteria Isolated from Passion Fruits

Biocellulose is a strong polymer consisting of nanofibrillar structures that produce a large surface area and a microporous structure. This organic polymer is greatly in demand in various industries, such as the paper industry, biomedical industry and cosmetics industry. In this study, biocellulose production from two bacteria known as endophytic bacterium SV845 (M02) and Pantoea ananatis IADCAMBID (M03) isolated from passion fruit was selected. Three different media formulations (Media 1, Media 2, and Media 3) were used in order to optimize the biocellulose production where each media contained a different percentage of carbon sources (glucose and fructose). The highest biocellulose production (1.544 mg/mL) was demonstrated by M02 bacteria strain in Media 1 containing glucose alone which fermented at 30 ºC while at 37 ºC, the highest biocellulose (BC) production was demonstrated by M03 bacteria strain at 2.078 mg/mL in media containing glucose alone (Media 1). Data on pH changes during biocellulose fermentation in all the media were set at an initial pH of 6. The final pH values were observed in the range of 5.34 to 6.08 for M02 strain and 5.95 to 8.53 for M03 strain, respectively. Characterizations of biocellulose were compared to starch using Fourier Transform Infrared (FTIR) spectroscopy. FTIR analysis indicated that the absorption peaks at 3200 cm-1 and 1630 cm-1 were derived from the association of intermolecular and intramolecular hydrogen bonds and H–O–H bending vibration of the absorbed water molecules in cellulose.

Valorization of pea pod, celery root peel, and mixed-vegetable peel as a feedstock for biocellulose production from Komagataeibacter hansenii DSM 5602

Valorization of pea pod, celery root peel, and mixed-vegetable peel as a feedstock for biocellulose production from Komagataeibacter hansenii DSM 5602

Optimization of Bacterial Nano-Cellulose Production in Bench-Scale Rotating Biological Contact Bioreactor by Response Surface Methodology

The main challenge in bacterial cellulose nanofibers production is low yield and high cost. The aim of this work is to optimize bacterial nano-cellulose production in the bench-scale rotating biofilm contact (RBC) bioreactor using experimental design. At all of experiments the Acetobacter Xylinum BPR2001 and culture medium molasses – CSL were used. Three effective factors in the three levels including rotation (10, 13 and 16 rpm), aeration (0.2, 0.5 and 0.8 vvm) and disk distance (1, 1.5 and 2 cm) were optimized by response surface experimental design. The optimum conditions of biocellulose production were rotation rate 13 rpm, aeration 0.5 vvm and disk distance 1.5 cm. The maximum dry weight of bacterial cellulose production reached 11.65 g/l in the 7th day, Which is one of the highest amounts of bacterial cellulose ever reported. Reduced quadratic models were used to final dry weight and moisture content of bacterial cellulose responses. ANOVA results showed the p-values were less than 0.05 that are significant models.

Properties and aerogel applications of a marine algal origin biocellulose produced by the immobilized Gluconacetobacter xylinus ATCC 10245

Algae are known to produce biomass faster as compared with lignocellulosic biomass. BC was produced by using reducing sugar from algae as a cheaper carbon source. The effect of immobilization on the production of biocellulose(BC) by Gluconacetobacter xylinus ATCC 10245 was studied. The BC production of all entrapped cultures within different gel materials was lower than that of free cell cultures but the entrapped G. xylinus cells in alginate beads recorded the highest BC productivity (12.5 g/l). The adsorption of G. xylinus cells on luffa pulp (LP) and ceramics particles (CP) gave the highest BC production (14.5 g/l), which represents increasing of 1.14 and 1.05- fold, respectively from the free cells. This attempt clarified that LP was the best immobilizing support as it gave the highest BC production. The effect of repeated reuse of LP was confirmed. Different physical properties of BC including; pH, water absorption capacity, dissolving in several solvents, and chemicals were determined. Observation of BC through SEM analysis showed the fine cellulose ribbons (fibrils). The crystallinity for the BC produced from X-ray diffraction (XRD) spectra was 84%. Upon the applicable level, the BC aerogel was formed to be applied in rising up the water and oil absorption capability by developing its hydrophilic properties. Data introduced strong affinities to the motor, olive, and cooking oils with high oil uptake contents of 7.5, 5.2, and 3.7 gg−1, respectively. Moreover, the BC aerogel exhibited potential adsorption for different heavy metal ions Pb2+, Cd2+, and Cu2+. The highest capability of Cd2+ removal was 95.7% at pH 6. The biodegrability of our produced BC and then its safety was proven.

Statistical optimization and characterization of a biocellulose produced by local Egyptian isolate Komagataeibacter hansenii AS.5

Optimization of the culture parameters used for biocellulose (BC) production by a previously isolated bacterial strain (Komagataeibacter hansenii AS.5) was carried out. The effect of nine culture parameters on BC production was evaluated by implementing the Plackett-Burman design, and the results revealed that, the most significant variables affecting BC production were MgSO4, ethanol, pH and yeast extract. A three-level and four-factor Box–Behnken design was applied to determine the optimum level of each significant variable. According to the results of the Plackett–Burman (PBD) and Box–Behnken designs (BBD), the following medium composition and parameters were calculated to be optimum (g/l): glucose 25, yeast extract 13, MgSO4 0.15, KH2PO4 2, ethanol 7.18 ml/l, pH 5.5, inoclume size 7%, cultivation temperature 20 °C and incubation time 9 days. Characterization of purified BC was performed to determine the network morphology by scanning electron microscopy, crystallinity by X-ray diffraction, chemical structure and functional groups by Fourier-transform infrared spectroscopy, thermal stability by thermogravimetric analysis and mechanical properties such as Young's modulus, tensile strength and elongation at beak % of BC.

Bio-cellulose Production by Beijerinckia fluminensis WAUPM53 and Gluconacetobacter xylinus 0416 in Sago By-product Medium.

Bio-cellulose is the microbial extracellular cellulose that is produced by growing several microorganisms on agriculture by-products, and it is used in several food applications. This study aims to utilize sago by-product, coconut water, and the standard medium Hestrin-Schramm as the carbon sources in the culture medium for bio-cellulose production. The bacteria Beijerinkia fluminensis WAUPM53 and Gluconacetobacter xylinus 0416 were selected based on their bio-cellulose production activity. The structure was determined by Fourier transform infrared spectroscopy and scanning electron microscopy, while the toxicity safety was evaluated by brine shrimp lethality test. The results of Fourier transform infrared spectroscopy showed that the bio-cellulose produced by B. fluminensis cultivated in sago by-products was of high quality. The bio-cellulose production by B. fluminensis in the sago by-product medium was slightly higher than that in the coconut water medium and was comparable with the production in the Hestrin-Schramm medium. Brine shrimp lethality test confirmed that the bio-cellulose produced by B. fluminensis in the sago by-product medium has no toxicity, which is safe for applications in the food industry. This is the first study to determine the high potential of sago by-product to be used as a new carbon source for the bio-cellulose production.

PH Effect and pH Changes during Biocellulose Production by Gluconacetobacter xylinus in Moringa oleifera Tea-Sugar Medium

pH Effect and pH Changes during Biocellulose Production by Gluconacetobacter xylinus in Moringa oleifera Tea-Sugar Medium

Design and Production Control of Biocellulose from <i>Acetobacter xylinum</i>

Background/Objectives: This research aims to design a bioreactor control system for the production of biocellulose from fermented matured coconut water using Acetobacter xylinum. Methods/Statistical Analysis: The parameters for this research include pH, volume and concentration of glucose in the medium within a nine-day productivity of fermentation and incubated at static condition in 250 ml of conical flask. Findings: Based on the research, the productivity of biocellulose increases with the volume of the medium and the concentration of glucose in the medium decrease with time. The best pH value for producing biocellulose was pH 4.5 and pH decrease with time. Based on the stoichiometric equation, glucose was the limited reactant and degree of freedom is two. The controlled manipulated variable for this research was volume of the medium and pH level. A set of fermenter with controller produced higher productivity than a set of fermenter without controller. The average dry weight of biocellulose produced was 2.46 g at pH 4.5 and 30°C. Application/Improvements: Therefore, the fermentation process with the control system is more efficient with biocellulose production of 69.65% compare to that without controller system.

Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits.

Biocellulose (BC) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from Malaysian acidic fruits. One hundred and ninety-three bacterial isolates were obtained from 19 local acidic fruits collected in Malaysia and screened for their ability to produce BC. A total of 15 potential bacterial isolates were then cultured in standard Hestrin-Schramm (HS) medium statically at 30°C for 2weeks to determine the BC production. The most potent bacterial isolates were identified using 16S rRNA gene sequence analysis, morphological and biochemical characteristics. Three new and potent biocellulose-producing bacterial strains were isolated from soursop fruit and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. Stenotrophomonas maltophilia WAUPM42 was the most potent biocellulose-producing bacterial strain that produced the highest amount of BC 0·58gl(-1) in standard HS medium. Whereas, the isolates P.vagans WAUPM45 and B.fluminensis WAUPM53 showed 0·50 and 0·52gl(-1) of BC production, respectively. Biocellulose (BC) is pure extracellular cellulose that is formed by many micro-organisms in the presence of carbon source and acidic condition. It can replace plant-based cellulose in multifarious applications due to its unique characteristics. In this study, three potential biocellulose-producing bacterial strains were obtained from Malaysian acidic fruits and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. This study reports for the first time the new biocellulose-producing bacterial strains isolated from Malaysian acidic fruits.

Statistical optimisation of culture conditions for biocellulose production by Komagataeibacter sp. PAP1 using soya bean whey

A low-cost medium based on soya bean whey (SBW) was prepared as a by-product from agriculture and optimised for biocellulose (BC) production by Komagataeibacter sp. PAP1. The optimal conditions for BC production are: pH 6.21, 1.61% ethanol concentration (v/v) and 28.4 o C. The use of optimised medium based on SBW increased BC production 3.6 fold compared to standard Hestrin-Schramm (HS) medium. The BC film produced from the optimised medium was stronger and more impermeable to water vapour and oxygen compared to the one produced from the standard HS medium. These properties allow SBW film to be developed into eco-friendly food packaging for oxygen-sensitive products. Its high water absorption capacity allows its use in biomedical application such as wound dressing. Our results demonstrate that SBW can be used as an alternative low-cost substrate for BC production on commercial scale.

Production of green biocellulose nanofibers by Gluconacetobacter xylinus through utilizing the renewable resources of agriculture residues

The present study demonstrates the ability to produce green biocellulose nanofibers using the renewable resources of agriculture residues. Locally grown wheat straws (WS) were hydrolyzed under different conditions. Their hydrolysates were utilized to produce the nanofibers in separate hydrolysis fermentation process by Gluconacetobacter xylinus strain bacterium. Highest biocellulose production of ~10.6 g/L was achieved with samples that were enzymatically hydrolyzed. Moreover, acidic hydrolyzed WS produced up to 9.7 g/L, with total sugar concentrations in culture media of 43 g/L. Generally, enzymatic hydrolysis of WS resulted in more total sugar concentration than the acidic hydrolysis (i.e., 52.12 g/L), while water hydrolysis produced the least. This can be related to utilizing Xylanase in addition to Cellulase and Beta-glucosidase that helps to hydrolyse WS dry basis of cellulose and hemicelluloses. Sugar mixtures produced under all hydrolysis conditions were mainly composed of glucose and xylose with average percentages of 56 and 28 %, respectively. Acidic hydrolysis at higher acid concentration, as well as soaking WS in the acidic solution for longer time, improved the total sugar concentration in the culture media by 18 %. Conducting thermal treatment at more intense conditions of higher temperature or heating time improved the total sugar produced with acidic hydrolysis. These conditions, however, resulted in further production of furfural, which considerably affected bacterial cells proliferation. This resulted in lowest sugar consumption in the range of 62-64 % that affected final BC production.

Potential of Biocellulose Nanofibers Production from Agricultural Renewable Resources: Preliminary Study

In the present preliminary study, we report results for the biocellulose nanofibres production by Gluconacetobacter xylinus. Production was examined by utilizing different feedstocks of single sugars and sugar mixtures with compositions similar to the acid hydrolyzates of different agriculture residues. Profiles for cell proliferation, sugar consumption, and the subsequent pH changes were thoroughly analyzed. Highest biocellulose production of 5.65 g/L was achieved in fructose medium with total sugar consumption of 95.57%. Moreover, the highest production using sugar mixtures was 5.2 g/L, which was achieved in feedstock with composition identical to the acid hydrolyzate of wheat straws. This represented the highest biocellulose yield of 17.72 g/g sugars compared with 14.77 g/g fructose. The lowest production of 1.1 and 1.75 g/L were obtained in xylose and glucose media, respectively, while sucrose and arabinose media achieved relatively higher production of 4.7 and 4.1 g/L, respectively. Deviation in pH of the fermentation broths from the optimum value of 4-5 generally had marked effect on biocellulose production with single sugars in feedstock. However, the final pH values recorded in the different sugar mixtures were approximately 3.3-3.4, which had lower effect on production hindrance. Analyzing profiles for sugars' concentrations and cell growth showed that large amount of the metabolized sugars were mainly utilized for bacterial cell growth and maintenance, rather than biocellulose production. This was clearly observed with single sugars of low production, while sugar consumption was rather utilized for biocellulose production with sugar mixtures. Results reported in this study demonstrate that agriculture residues might be used as potential feedstocks for the biocellulose nanofibres production. Not only this represents a renewable source of feedstock, but also might lead to major improvements in production if proper supplements and control were utilized in the fermentation process.

The evaluation of carrier material for increasing qualities of gel inoculum for nata de coco

Producion of nata de coco is growing fast, in line with increasing product of biocellulose demand. It is requrire a pure inoculum to reach the best biocellulose product. The aim of this reseach is to evaluate the most appropriate carrier material of gel inoculum for nata de coco. The four carrier material are Carboxy Methyl Cellulose (CMC), Agar, Sagu starch and biocellulose pap and its inoculated by Acetobacter sp. RMG-2 and A. xylinum. After inoculation, then put in the plastic bag (50 g/bag) and stored in 4° C. The texture of gel, population of cell andbiocellulose production were observer in 7 days. The result shown that all matterial were suitable to used as carrier for gel inoculum. Both CMC and biocellulose pap have good texture as standard qualities. Population of A. xylinum was 1.28x109 cfu/mL (in CMC carrier), 1.6x106 cfu/mL (in biocellulose pap carrier) after 15 weeks. The weight of biocellulose production was 500 g/L and 740 g/L medium respectively. While the population of Acetobacter sp. RMG-2 on CMC carrier was 1.79x108 cfu/mL and 7.75x107 cfu/mL on cellulose carrier with the weight of biocellulose production 630 g/L and 775 g/L medium respectively. Thus, the carrier material (CMC and Biocellulose pap) are able to keep bacteria without loss their capability to produce cellulose.Key words: Acetobacter sp. RMG-2, Acetobacter xylinum, carrier material, gel inoculum, nata de coco.