Bioavailability Of Micronutrients Research Articles

Recommendations to address the shortfalls of the EAT-Lancet planetary health diet from a plant-forward perspective.

Shifting to dietary patterns rich in plants and low in animal-source foods could substantially lower emissions from the food sector while reducing the global burden of non-communicable diseases. The EAT-Lancet Commission proposed the planetary health diet (PHD) to emphasise plant-forward diets and set global targets to guide an urgently needed food-system transformation. However, the PHD's meat-reduction approach has attracted criticism and prompted debate on the potential micronutrient shortfalls of the plant-forward dietary approach. Since the planet simply cannot sustain human diets defaulting towards animal-based solutions, the objective of this Viewpoint is to provide recommendations that address the shortfalls of the PHD, with an emphasis on plant-based sourcing of food. Using a socioecological approach, along with an Integrative Sustainability Framework to evaluate dietary guidelines, in this Viewpoint we recommend seven key thematic areas for further development of the PHD. These themes relate to the bioavailability of micronutrients from plant-based foods, the inclusion of indigenous foods and practices, fortification and supplementation, cultural inclusiveness, and gender-based differences, a broader perspective on processed foods, and strengthening the concept by integrating the One Health approach.

A Novel Non-Alcoholic Einkorn-Based Beverage Produced by Lactic Acid Fermentation: Microbiological, Chemical, and Sensory Assessment.

Einkorn (Triticum monococcum L. ssp. monococcum) is gaining renewed interest for its high nutritional value and digestibility. Lactic acid fermentation could enhance these properties by improving micronutrient bioavailability, sensory properties, and shelf life. This study aimed to develop a novel non-alcoholic einkorn-based beverage through lactic acid fermentation. A multiple-strain starter was selected based on acidifying properties and inoculated into an einkorn-based substrate to produce a yogurt-like beverage. Prototypes were evaluated through physico-chemical, chemical, and microbiological analyses and compared to uninoculated controls. A sensory analysis was also performed to assess flavor attributes before and after lactic acid fermentation. The inoculated starter culture reached a load of approximately 9 Log CFU g⁻¹ and remained viable throughout storage, leading to an increase in lactic acid concentration and high titratable acidity, corresponding to low pH values. Total polyphenol content increased during fermentation and remained stable during storage, whereas antioxidant activity did not show significant differences over time. An increase in monosaccharides, acids, and ketones was observed during fermentation and storage. The prototypes exhibited a distinctive proximate composition, along with yogurt and fruity aroma notes. These results suggest the feasibility of producing a safe and stable non-alcoholic einkorn-based fermented beverage with appealing sensory characteristics.

Phytate content and micronutrient bioavailability of cereal and pulse products available in Australian supermarkets

Phytate content and micronutrient bioavailability of cereal and pulse products available in Australian supermarkets

Dietary Phytic Acid, Dephytinization, and Phytase Supplementation Alter Trace Element Bioavailability-A Narrative Review of Human Interventions.

Phytic acid is abundant in plant-based diets and acts as a micronutrient inhibitor for humans and non-ruminant animals. Phytases are enzymes that break down phytic acid, releasing micronutrients and enhancing their bioavailability, particularly iron and zinc. Deficiencies in iron and zinc are significant public health problems, especially among populations with disease-associated malnutrition or those in developing countries consuming phytic acid-rich diets. This narrative review aimed to summarize findings from human intervention studies on the interactions between phytic acid, phytase, and micronutrient bioavailability. An extensive PubMed search (1 January 1990 to 8 February 2024) was conducted using MeSH terms (phytic acid, phytase, IP6, "inositol hexaphosphate," micronutrient, magnesium, calcium, iron, zinc). Eligible studies included human intervention trials investigating the bioavailability of micronutrients following (a) phytase supplementation, (b) consumption of phytic acid-rich foods, or (c) consumption of dephytinized foods. In vitro, animal, cross-sectional, and non-English studies were excluded. 3055 articles were identified. After the title and full-text review, 40 articles were eligible. Another 2 were identified after cross-checking reference lists from included papers, resulting in 42 included articles. Most studies exploring the efficacy of exogenous phytase (9 of 11, 82%) or the efficacy of food dephytinization (11 of 14, 79%) demonstrated augmented iron and zinc bioavailability. Most phytic acid-rich food-feeding studies (13 of 17, 77%) showed compromised iron and zinc bioavailability. Strong evidence supports decreased iron and zinc bioavailability in phytic acid-rich diets and significant improvements with phytase interventions. Studies of longer periods and within larger populations are needed.

Selection and immobilization of the optimal phytase-producing probiotic: a comparative in silico analyses of five autochthonous lactic acid bacteria

Phytic acid and its salts are the major storage form of both phosphate and myo-inositol, especially in cereals. Phytic acid chelates multivalent cations, which decreases the solubilized form of these nutrients and their dietary bioavailability for absorption and assimilation. Increasing micronutrient bioavailability from the economic phytate-rich food can be achieved by subjecting this food to fermentation by phytase-producing bacteria. A total of 8 lactic acid bacteria were isolated from different fermented food samples. Among the bacteria tested for their ability to produce phytase, only five isolates were selected as promising producers. The results revealed that isolate No. 4 produced the highest phytase levels of 411 U/mL. The five isolates were differentially able to tolerate acidic, alkaline, heat, surfactant, osmotic, bile, and pancreatic enzyme stresses, with the superiority of isolate No. 4, which demonstrated the most desirable probiotic potentials as confirmed by principal component analysis (PCA), heat map, and network analysis. Isolate No. 4 was identified by 16S rRNA sequencing and later confirmed as Pediococcus pentosaceus strain NMP4762Ch under the accession number MZ413646. Moreover, the active cells of P. pentosaceus were immobilized in alginate; the reusability of the immobilized active cells resulted in continuous production with an optimum phytase activity of 432.0 U/mL after 14 days, which decreased with time to reach 142.5 U/mL after 56 days. The maximum shelf stability was also observed, 557.5 U/mL, after 14 days and declined with time, reaching 133.5 U/mL by the end of the 56th day. Since humans don’t naturally produce phytase, utilizing probiotics for phytase production is important to combat mineral deficiencies.

Enhancing iron and zinc uptake in spring wheat through commercial arbuscular mycorrhizal fungi inoculation under different soil phosphorus addition levels

Improving the concentrations and bioavailability of micronutrients, especially iron (Fe) and zinc (Zn), in crop grains is important to alleviate their deficiencies in humans. Inoculating crops with arbuscular mycorrhizal fungi (AMF) can potentially enhance soil nutrient supply and crop yield, but the effectiveness is influenced by soil factors, particularly soil phosphorus (P) availability. A greenhouse pot experiment was conducted to evaluate the effect of a commercial AMF product on spring wheat ( Triticum aestivum L.) yield and grain concentrations of Zn and Fe under different soil P addition levels (0, 5, and 20 mg P kg–1 dry soil). Results showed that AMF inoculation significantly increased root colonization rate of wheat across all P addition levels. Wheat growth, as evidenced by dry weights of shoot and grain, was significantly enhanced by AMF and high P addition treatments. AMF inoculation did not affect grain Zn concentration, but significantly increased grain Fe concentration compared to the un-inoculated control. As expected, P addition resulted in a significant reduction in grain concentrations of Fe and Zn, primarily due to a growth dilution effect. An integrated analysis using the radar plot concludes that AMF inoculation is most effective in increasing crop yield and grain micronutrient concentrations when soil P levels are low. Importantly, while adequate P supply is crucial for maintaining crop productivity, it may decrease grain micronutrient availability without complementary strategies.

High doses of polypropylene and polyvinyl chloride microplastics affect the microbial community and nutrient status of vineyard soils

The escalating use of plastic materials in viticulture causes release of microplastics (MPs) into vineyard soils. This study examines the impact on soil health of polypropylene (PP) raffia and polyvinyl chloride (PVC) tube strings, commonly mulched into the topsoil after use. A 120-d incubation experiment was conducted with soils exposed to high doses (10 g/kg) of microplastics (MPs) from standard, new and used strings. The study investigated alterations in the microbial community, bioavailability of macronutrients (NH4+ and NO3−, P, K, Ca, Mg), and bioavailability of micronutrients (Cu, Zn, Fe, Mg). The presence of MPs significantly stressed the soil microbial community, reducing microbial biomass by 30% after 30 d, with the exception of PVC in acid soil, which caused an unexpected increase of about 60%. The metabolic quotient (qCO2) doubled in MP-polluted soils, with PVC exerting a more pronounced effect than PP. Basal respiration increased by 25% relative to the acid control soil. PVC MPs raised soil pH from 6.2 to 7.2 and firmly reduced the bioavailability of micronutrients, particularly in acidic soils, and led to a 98% reduction in nitrate (NO3−). The availability of NH4+, P, K, Mg decreased by 10% and Cu, Fe, Mn, Zn by 30%. However, Ca availability increased by 30%, despite shifting from the acid-soluble fraction to soil organic matter and crystalline minerals. Calcareous soil was generally more resilient to changes than the acid soil. These findings underscore the urgent need to investigate the long-term effects of MPs from viticulture on soil properties and health.

Nutrition and health effects of pectin: A systematic scoping review of human intervention studies.

Pectin is composed of a group of complex polysaccharides that are naturally found in various plants and are associated with a range of beneficial health effects. Health outcomes from dietary pectin can vary depending on botanical origin, dietary dose and structure of pectin. The objective of this scoping review is to build a comprehensive overview of the current evidence available on intervention studies conducted in humans and to better understand the possible knowledge gaps in terms of structure-function relationships across the different health-related effects. PubMed and Embase databases were searched using PRISMA-ScR guidelines, yielding 141 references (from the initial 3704), representing 134 intervention studies performed between 1961 and 2022 that met inclusion criteria. Studies were divided into six categories, which included gut health, glycaemic response and appetite, fat metabolism, bioavailability of micronutrients, immune response and other topics. Review of these human intervention studies identified a variety of cohort characteristics and populations (life stage, health status, country), sources/types of pectin (i.e. citrus, sugarbeet, apple, other and non-defined), intervention timeframes (from one single intake to 168 d) and doses (0.1-50 g/d) that were tested for health outcomes in people. Gut health, post-prandial glucose regulation and maintenance of blood cholesterol represented the largest categories of studied outcomes. Further research to strengthen the structure-function relationships for pectin with health properties and associated outcomes is warranted and will benefit from a more precise description of physico-chemical characteristics and molecular compositions, such as degree of esterification, weight, degree of branching, viscosity, gel formation and solubility.

Development and characterization of lpa1 and lpa2-based low phytate double mutants in maize for enhancing the nutritional quality of food and feed

Phytic acid (PA), an anti-nutritional factor hampers the nutritional value of maize-based food by reducing the bioavailability of zinc and iron. Low phytate maize is key for addressing poor micronutrient bioavailability. Here, we developed low phytic acid double mutants (lpa1/lpa2) in maize by crossing lpa1 and lpa2-based near-isogenic lines (NILs) in seven genetic backgrounds. Genotypic selections for lpa1 and lpa2 genes in the F2 population revealed segregation ratios of 9:3:3:1 for double mutants and 3:1 for single mutants. Newly developed double mutants along with single mutants and wild types, were field-evaluated for two seasons. Wide genetic variation was observed for PA, inorganic phosphorus (Pi), grain yield, and agro-morphological traits, with negligible G×E interactions. PA concentration of lpa1/lpa2 (1.73 mg/g) was significantly (P <0.05) lower than wild types (2.92 mg/g), lpa1 (1.86 mg/g), and lpa2 (2.05 mg/g) lines. The double mutants revealed more prominent PA reduction (41 %) than single mutants, lpa1 (36 %) and lpa2 (30 %) compared to wild types. Additionally, the double mutants recorded comparable performance to wild types for grain yield and other agro-morphological traits. This study illustrated the potential of double mutants over lpa-based single mutants in lowering the anti-nutritional effects and enhancing the nutritional value of maize grains.

Stability of metal ion complexes with the synthetic phytosiderophore proline-2'-deoxymugineic acid.

Adequate micronutrient concentrations in crops are essential for human health and agricultural productivity. However, 30% of plants growing on cultivated soils worldwide are deficient in iron (Fe). Because of low micronutrient bioavailability, graminaceous plants have evolved to exude small molecules, called phytosiderophores, into the soil environment, which strongly complex and promote uptake of trace elements. The development of a synthetic phytosiderophore, proline-2'-deoxymugeneic acid (PDMA), has been shown to promote Fe uptake in rice plants; however, its binding capabilities with other metals, which may impact the ability to promote the uptake of Fe and other trace nutrient metals commonly found in soils, remain unknown. We conducted spectrophotometric titrations to determine the stability constants (logK) of PDMA complexes with Mn(II), Co(II), Cu(II), Ni(II), and Zn(II). We determined that PDMA complex stability constants correlated with: (1) the hydrolysis constants of metal ions (logKOH) in complexes; (2) the ionic potential of complexed metals; and (3) the corresponding complex stability constants of other mugineic acid type phytosiderophores, as well as the trishydroxamate microbial siderophore DFOB. These correlations demonstrate the potential, and limitations, on our ability to predict the stability of phytosiderophore complexes with metal ions with different physicochemical properties and with potentially different coordination structures.

Opportunities for Blue food in a turbulent future - what’s ahead for a growing aquaculture industry?

The scale and speed of human activities and technological developments have substantially increased since the 1950s resulting in reshaping of the biosphere and moving humanity into a new geological epoch — the ”Anthropocene”. This is characterized by changes in several Earth system processes and structures on which human life depends. The global food system is a major driver of changes in the biosphere – for example greenhouse gas emission and other environmental changes including global biodiversity loss. Blue foods (aquatic foods) have slowly made their way into international high-level sustainability discussions and increasingly now form part of nutritious, climate smart and low environmental impact food narratives. Today farmed and captured aquatic animals and plants play a central role in food and nutrition security for billions of people, and constitute cornerstones for many livelihoods, economies, and cultures. The blue food portfolio is highly diverse and also supported by a wide range of ecosystems, cultural practices and production modalities. This diversity poses both potential for supporting food system resilience in uncertain times, and challenges for making simple guidance on sustainable development of food production. The anticipated continuous growth of aquaculture trigger questions related to “what, how and why”. Many blue foods are rich in bioavailable micronutrients and can be produced in ways that are more environmentally sustainable than some terrestrial animal-source foods. However, blue foods are diverse and planning for future expansion through i.e. aquaculture involves identification of properties for different species and systems, as well as identification of development priorities and acknowledging trade-offs and context specificity out from a broader Sustainable Development Goal framework. Environmental stressors may narrow the window for blue food production and climate change is a main driver affecting the function and productivity of aquatic and supportive terrestrial ecosystems.

Assessing the Protein Quality, In Vitro Intestinal Iron Absorption and Human Faecal Microbiota Impacts of Plant-Based Mince.

The nutritional quality of plant-based meat analogues compared to traditional meat products has been questioned in recent commentary, particularly in relation to protein quality and micronutrient bioavailability. However, the attributes of specific products within this category are unclear. We therefore undertook a comprehensive assessment of the compositional and functional attributes of v2food® (Sydney, Australia) plant-based mince, including an assessment of the effects of reformulation, including the addition of amino acids, ascorbic acid, and different forms of elemental iron. The protein digestibility and protein quality of v2food® plant-based mince were comparable to beef mince in the standardized INFOGEST system, and favourable effects on microbiota composition and short-chain fatty acid (SCFA) production were demonstrated in an in vitro digestion system. The use of ferrous sulphate as an iron source improved in vitro intestinal iron absorption by ~50% in comparison to other forms of iron (p < 0.05), although levels were ~3-fold lower than beef mince, even in the presence of ascorbic acid. In conclusion, the current study identified some favourable nutritional attributes of plant-based v2food® mince, specifically microbiota and SCFA changes, as well as other areas where further reformulation could be considered to further enhance the bioavailability of key nutrients. Further studies to assess the effect of plant-based meat analogues on health measures in vivo will be important to improve knowledge in this area.

Organic carbon, major and trace element release from and adsorption onto particulate suspended matter of the Ob River, western Siberia

Particulate suspended matter (PSM) of rivers is a significant factor for carbon, nutrient, and trace metal transfer from land to ocean. Towards better understanding the role that PSM exerts on major and trace elements in riverine systems, here we report the results of an experimental study which utilizes a two-fold approach to assess interaction between PSM and riverine solutes. First, we measured element leaching (via desorption and dissolution in distilled water, simulating snow melt) from PSM of the largest Siberian river, the Ob River. Second, we quantified the capacity of PSM to adsorb dissolved organic carbon (DOC), macro- and micronutrients and trace elements from organic-rich waters of the river floodplain. We documented sizable desorption of organic carbon, some major and trace metals, oxyanions and insoluble elements from PSM; the majority (>50 %) of elements were released over the first hour of reaction. In contrast, PSM of the Ob River was capable of removing 20 to 90 % of dissolved OC, nutrients (Si, P), and trace elements from the tributary and floodplain fen. Our experiments demonstrated preferential adsorption of aromatic compounds large molecular size colloids. Taken together, the adsorption of solutes by PSM can sizably decrease the concentration and modify the molecular size distribution, and therefore the potential bioavailability of major (DOC, P, Si) and trace micronutrients. Overall, the PSM of the Ob River exhibited high reactivity with respect to natural waters and was capable of modifying the elemental composition of the tributary and floodplain fen waters. This transfer of organic carbon and nutrients in the surface-adsorbed (particulate) form is especially important during spring flood and requires specific consideration in short-term biogeochemical cycles of elements in continental waters.

Proximal sensor characterization of Haitian agricultural soils: A case study in the Central Plateau

Optimized agronomic production is vital to life and livelihood in Haiti. As such, rapid, low-cost soil characterization may inform specific decision making for improved crop production. Ahead of a larger soil characterization campaign (120,000 points) in Eastern Haiti, this study evaluated the use of two proximal sensors (portable X-ray fluorescence – pXRF; NixPro color sensor) at farms of Association Zanmi Agrikol (AZA) for soil characterization and evaluation of spatial variability of soil properties. Analysis of 166 surface soil samples (0–5 cm) collected in three different areas revealed that similar inter-elemental associations reported in other countries remain applicable in Haiti; weak, yet significant relationships between Al, K, and Fe contents and color parameters (L*, A*, B*) were observed. Considerable variability in soil morphology and chemical properties exist even across short distances, underscoring the need for high density sampling schemes. Soil pH (observed up to 8.4) likely imposes restrictions on the bioavailability of certain micronutrients; soil salinity was low (not agronomically limiting) across the study areas, and reflective of excessive drainage. Soil profile characterization identified one Eutrudept and one Quartzipsamment; both used for agronomic production. The proliferation of skeletal coarse fragments (e.g., cobbles, stones) and sandy matrix with depth emphasize the need for periodic supplemental irrigation and fertilization via either soil amendments (e.g., compost) or commercial fertilizers as water holding capacity and nutrient storage (e.g., clays, organic matter) are likely to be limited. Use of the NixPro color sensor showed differences in topsoil color likely reflective of differential soil organic carbon levels. The proximal sensors applied herein represent their first such use for agronomic management in Haiti and have shown considerable promise for additional study and application.

Intrahousehold Food Distribution in the Philippines: A Food Share Over Energy Share Perspective.

Studies on intrahousehold inequality in food distribution typically have focused on the distribution of total energy within the household. While some members within the household can satiate their hunger primarily from the consumption of inexpensive staple food items, others can consume a significantly larger share of much more expensive nonstaple food items rich in bioavailable micronutrients. This potential inequality, which significantly impacts micronutrient malnutrition in developing countries, can be influenced by the gender-related sociocultural norms. This article uses a "food share over energy share (FS/ES)" methodology applied to the Philippines 2013 intrahousehold individual food consumption survey to examine patterns of dietary quality by age and gender. No pattern of age or gender discrimination is found; females consume most nonstaple foods somewhat more intensively than males. Preschoolers and young children relatively intensively consume higher amounts of milk and milk products, eggs, processed meat, noodles, sweets/desserts, sugars/syrups, and chocolate drinks. Adults relatively intensively consume fresh meat, organ meat, fish and seafood, vegetables, coffee, and alcohol. Adolescents transition between the two patterns as they grow older. The FS/ES methodology focuses on distribution choices of specific foods that family members explicitly know about. The implications of these behavioral choices (some foods are nutrient-dense, others not) for meeting nutrient requirements (of which most households are unaware) may be investigated separately. Several types of follow-up studies using the FS/ES methodology are suggested for a better understanding of the factors that drive food distribution behavior, which is key to effective nutrition messaging.

Mitigating potential of polystyrene microplastics on bioavailability, uptake, and toxicity of copper in maize (Zea mays L.)

The coexistence of polystyrene microplastics (PSMPs) and copper (Cu) has become a pressing issue for croplands. However, limited literature is available regarding the interaction of PSMPs with essential micronutrients in Cu-contaminated soils. Therefore, the present study aimed to analyze the immobilization potential of PSMPs for micronutrient bioavailability in soil and Cu toxicity in maize (Zea mays L.). A pot experiment was conducted with maize variety “Islamabad gold” exposed to varying Cu concentrations (0, 50, 100, 200, and 400 mg/kg) and PSMPs (150–250 μm size, 0, 1, and 3% w/w) via soil spiking for 60 days. The concentrations of essential micronutrients (Zn, Cu, Mn, Fe) in soil and plant tissues were measured using an atomic absorption spectrophotometer. Moreover, malondialdehyde (MDA) and antioxidant activities (superoxide dismutase, ascorbate peroxidase, catalase, and peroxidase) were recorded. The concentration of Cu showed significant reduction in post-harvesting soil by 21, 24.8, 27.6, 29.2, and 30.2% from Cu0 to Cu400 mg/kg respectively from pre-sowing soil. On the other hand, the addition of 1%PSMPs and 3%PSMPs declined Cu by 16, 21.6, 24.4, 25.9, 27.8, and 12.6, 16.5, 19.9, 23.2, 25% from Cu0 to Cu400 mg/kg respectively. Maize showed significant improvement in growth under combined exposure of Cu and 3% PSMPs compared to individual exposure. The MDA level was decreased under the combined presence of Cu and PSMPs compared to individual Cu exposure. The percentage difference with 1%PSMPs was 98.1, 95.0, 92.0, 90.0, and 89.6%, while with 3%PSMPs was 93.2, 93.2, 87.7, 81.4, and 79.2% from Cu0 to Cu400 mg/kg respectively. Moreover, the impact of PSMPs was more prominent at a 3% dose compared to a 1% dose. The findings provided significant knowledge about the potential of PSMPs to mitigate Cu toxicity in maize. Future research should incorporate a variety of particle size distributions at natural conditions for variety-specific differences.

Improving the antinutritional profiles of common beans (Phaseolus vulgaris L.) moderately impacts carotenoid bioaccessibility but not mineral solubility

Common beans are a common staple food with valuable nutritional qualities, but their high contents in antinutritional factors (ANFs) can decrease the bioavailability of (i) fat-soluble micronutrients including carotenoids and (ii) minerals. Our objective was to select ANF-poor bean lines that would not interfere with carotenoid and mineral bioavailability. To achieve this objective, seeds of commercial and experimental Phaseolus vulgaris L. bean lines were produced for 2 years and the bean’s content in ANFs (saponins, phytates, tannins, total polyphenols) was assessed. We then measured carotenoid bioaccessibility and mineral solubility (i.e. the fraction of carotenoid and mineral that transfer into the aqueous phase of the digesta and is therefore absorbable) from prepared beans using in vitro digestion. All beans contained at least 200 mg/100 g of saponins and 2.44 mg/100 g tannins. The low phytic acid (lpa) lines, lpa1 and lpa12 exhibited lower phytate levels (≈ − 80%, p = 0.007 and p = 0.02) than their control BAT-93. However, this decrease had no significant impact on mineral solubility. HP5/1 (lpa + phaseolin and lectin PHA-E free) bean line, induced an improvement in carotenoid bioaccessibility (i.e., + 38%, p = 0.02, and + 32%, p = 0.005, for phytofluene bioaccessibility in 2021 and 2022, respectively). We conclude that decrease in the phytate bean content should thus likely be associated to decreases in other ANFs such as tannins or polyphenols to lead to significant improvement of micronutrient bioaccessibility.

The fate and biostimulant potential of metal lactates in silt loam soil

AbstractConventional farming practices have negatively impacted micronutrient fertility and overall soil health. Metal lactates are an organic micronutrient amendment that provide both a labile carbon substrate as well as mineral nutrition for plant and microbial growth. To determine the ability of metal lactates to provide readily available micronutrients, Zn, Cu, Mn, Ni, and Co in lactate and chloride salt form were amended to soils and incubated for 28 days. Operationally defined speciation was determined using sequential extraction on samples incubated for 1, 5, or 28 days. The results show a comparable distribution of metals in chloride and lactate form; however, differences were detected in water‐soluble and exchangeable Ni and Zn at each timepoint. For example, in the water exchangeable fraction, there was 2.6%–2.9% less Ni and 0.2%–0.3% less Zn in the metal lactate‐treated soil compared to soil treated with metal chlorides. Furthermore, carbonate‐bound Ni averaged 4.1% and 4% less in metal lactate‐treated soils as compared to metal chloride‐treated soils on days 1 and 5, and Cu decreased 2.9%–3.3% during the treatment period for the lactate‐bound form. Additionally, microbial phospholipid fatty acid (PLFA) was performed and found a stimulatory effect of metal lactates on bacterial biomass with an increase of 12%–18% relative to the metal chloride treatment. Results from this study support the use of metal lactates as a sustainable alternative to conventional fertilizers by providing bioavailable micronutrients for plant nutrition in addition to a labile carbon source to support the growth of microbial populations.

Effect of selective fermentation on nutritional parameters and techno-functional characteristics of fermented millet-based probiotic dairy product

The primary goal of this study was to assess the effect of selective fermentation on the nutritional and techno-functional characteristics of fermented millet-skim milk-based product. The product was made with HHB-311 biofortified pearl millet (PM) flour, skim milk powder, and isolated cultures (either alone or in combination) of Limosilactobacillus fermentum MS005 (LF) and Lactobacillus rhamnosus GG 347 (LGG). To optimize fermentation time, time intervals 8, 16, and 24 h were explored, while the temperature was kept 37 °C. Results of protein digestibility showed that LF (16 h) and LGG (24 h) fermented samples had significantly higher (P < 0.05) protein digestibility of 90.75 ± 1.6% and 93.76 ± 3.4%, respectively, than that of control (62.60 ± 2.6%). Further, 16 h fermentation with LF showed enhanced iron (39%) and zinc (14%) bioavailability. The results suggested that LF with 16 h fermentation is most suitable for making millet-based fermented products with superior techno-functional attributes and micronutrient bioavailability.

The Role of Bio-fortification in Enhancing the Nutritional Quality of Vegetables: A Review

Bio-fortification is a process that enhances the nutritional quality of crops, including vegetables, by increasing their micronutrient content. It can be achieved through different approaches such as agronomic, conventional breeding, and transgenic/biotechnological methods. Vegetables are known to be rich in micronutrients, vitamins, antioxidants, and other health-benefiting compounds, making them essential for a balanced diet. However, malnutrition and hidden hunger continue to be global challenges, particularly in developing countries. Micronutrient deficiencies, such as iron, zinc, iodine, and vitamin A, are prevalent in these populations. Conventional breeding focuses on selecting genotypes with desirable nutritional traits without compromising agricultural productivity. Transgenic/biotechnological approaches involve the synthesis of transgenes to enhance the bioavailability of micronutrients in plants. Bio-fortification of vegetables is particularly important as they are rich sources of micronutrients, vitamins, and other health-benefiting compounds. However, improving the nutritional quality of vegetables through conventional breeding has had limited success, and modern molecular tools and techniques offer potential for handling complex traits and developing nutrient-dense varieties. Bio-fortification offers a sustainable solution to address these deficiencies by increasing the nutrient content of crops, particularly plant-based foods. These techniques have shown promising results in increasing the concentration of nutrients, such as iron, in vegetables, thereby improving their nutritional quality.