Bioactive Polyphenolic Compounds Research Articles

Perception into the binding of soy protein isolate with essential oils using multispectroscopic and QuickVina-W

This study aimed to explore how soy protein isolate (SPI) binds with two types of essential oils (cumin (Cuminum cyminum) (CUM) and coriander (Coriandrum sativum) seed (CO)) and how it affects protein conformation. The results showed that SPI formed stable ternary complexes with CUM and CO that were less than 100 nm in size and had a zeta potential of −15 mV. The successful encapsulation of both CUM and CO within the SPI was confirmed through fourier-transform infrared spectroscopy, X-Ray diffraction analysis, and differential scanning calorimetry tests. These tests showed that both CUM and CO were effectively contained within SPI. The addition of CUM and CO to SPI significantly (p < 0.05) enhanced the ability of small molecules to scavenge ABTS, and this improvement was maintained at a relatively constant level during irradiation for 160 min. These results could be useful in utilizing SPI as an effective co-delivery system for bioactive polyphenol compounds that vary in hydrophobicity and molecular weight.

The Effects of Green Tea Catechins in Hematological Malignancies.

Green tea catechins are bioactive polyphenol compounds which have attracted significant attention for their diverse biological activities and potential health benefits. Notably, epigallocatechin-3-gallate (EGCG) has emerged as a potent apoptosis inducer through mechanisms involving caspase activation, modulation of Bcl-2 family proteins, disruption of survival signaling pathways and by regulating the redox balance, inducing oxidative stress. Furthermore, emerging evidence suggests that green tea catechins can modulate epigenetic alterations, including DNA methylation and histone modifications. In addition to their apoptotic actions, ROS signaling effects and reversal of epigenetic alterations, green tea catechins have shown promising results in promoting the differentiation of leukemia cells. This review highlights the comprehensive actions of green tea catechins and provides valuable insights from clinical trials investigating the therapeutic potential of green tea catechins in leukemia treatment. Understanding these multifaceted mechanisms and the outcomes of clinical trials may pave the way for the development of innovative strategies and the integration of green tea catechins into clinical practice for improving leukemia patient outcomes.

Tannic acid-layered hydroxide salt hybrid: assessment of antibiofilm formation and foodborne pathogen growth inhibition.

Pathogenic bacteria in food are a public health problem worldwide. Polyphenolic bioactive compounds with antimicrobial activity and antioxidant capacity represent a tangible alternative to overcome this problem. To preserve the biological functions of phenolic compounds such as tannic acid, which has been described to possess antioxidant and antimicrobial activity, this study describes the synthesis of a zinc nanohydroxide to stabilize its properties. Characterization by XRD, FT-IR, SEM, DLS, and UV-vis evidenced the presence of tannic acid in the nanohybrid TA-Zn-LHS which was further confirmed by DPPH, ABTS and FRAP antioxidant activity techniques. Bacterial growth inhibition of Escherichia coli ATCC 8739, Salmonella Enteritidis, and Staphylococcus aureus ATCC 25923 was over 80% at 50mg/mL of the TA-Zn-LHS and over 90% with Zn-LHS. Antibiofilm evaluation of these same strains showed biofilm formation inhibition > 90% and > 80% for Zn-LHS and TA-Zn-LHS, respectively. The toxicity evaluation of the materials in Artemia salina showed a classification of the materials as non-toxic to slightly toxic in concentrations up to 1mg/mL. These results allow us to introduce a new nanohybrid useful for food safety with safe biological functions.

Valorization of olive leaf waste as a new source of fractions containing cellulose nanomaterials

The olive oil activity generates annually a huge amount of biomass waste from the pruning action of the tree and fruit harvesting, including thin woody branches and leaves. In an effort toward more sustainability in the lignocellulosic biorefinery of olive waste activity, nanocelluloses (NCs) were produced from the solid residues remaining after the extraction of biological active compounds from the leaves. Fractions containing cellulose nanocrystals (CNCs), cellulose nanofibrils (CNFs), and lignin-containing cellulose nanofibrils (LCNFs) were prepared and characterized in terms of morphology, chemical composition, thermal stability, colloidal properties, and crystallinity. The reinforcing capability of the different fractions was evaluated in nanocomposite films made of an acrylic matrix incorporating different NC contents. Dynamic mechanical analysis (DMA) and tensile testing of the films were used to compare the reinforcing effect of the NCs. Although CNCs displayed the highest reinforcing effect based on the weight content, LCNFs and CNFs demonstrated also a reinforcing effect, which is higher when normalized with the cellulose content. Compared to CNFs and CNCs, LCNFs displayed a lower hydrophilic character confirmed by contact angle measurement, and had better thermal stability. This work demonstrated the potential use of the solid waste from olive leaves as a new source for biobased nanoparticles containing lignin, hemicellulose components, and cellulose nanofibrils. Beside the high content in bioactive polyphenol compounds that can be extracted from olive leaves, the conversion of the residual biomass can contribute to increasing the circularity and sustainability of olive oil activity.

Short-Term Effects of PJE Administration on Metabolic Parameters in Diet-Induced Obesity Mice.

The study investigated the effects of Petasites japonicus (Siebold & Zucc.) Maxim. extract (PJE) and fenofibrate on diet-induced obesity (DIO) in mice. PJE was found to contain various bio-active polyphenolic compounds, including kaempferol, p-hydroxybenzoic acid, ferulic acid, gallic acid, chlorogenic acid, 3,4-dicaffeoylquinic acid, caffeic acid, quercetin, rutin, protocatechuic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, p-coumaric acid, apigenin, and 1,3-dicaffeoylquinic acid. The results showed that PJE treatment up to 1000 μg/mL did not affect the viability of 3T3-L1 cell line, and it reduced the feed efficiency ratio in DIO mice. PJE administration also resulted in a significant reduction in body weight gain and fat accumulation in the liver compared to the DIO control group. Additionally, PJE administration improved the levels of lipid and related parameters, including total cholesterol, triacylglycerol, low-density lipoprotein, very low-density lipoprotein, glucose, insulin, insulin resistance, leptin, and atherogenic or cardiac indexes compared to the DIO control group. The study suggested that PJE may have a beneficial effect on insulin resistance, lipid profiles, atherogenesis, adipokines, and cardiac risk associated with diet-induced obesity.

Role of Hydroxytyrosol and Oleuropein in the Prevention of Aging and Related Disorders: Focus on Neurodegeneration, Skeletal Muscle Dysfunction and Gut Microbiota.

Aging is a multi-faceted process caused by the accumulation of cellular damage over time, associated with a gradual reduction of physiological activities in cells and organs. This degeneration results in a reduced ability to adapt to homeostasis perturbations and an increased incidence of illnesses such as cognitive decline, neurodegenerative and cardiovascular diseases, cancer, diabetes, and skeletal muscle pathologies. Key features of aging include a chronic low-grade inflammation state and a decrease of the autophagic process. The Mediterranean diet has been associated with longevity and ability to counteract the onset of age-related disorders. Extra virgin olive oil, a fundamental component of this diet, contains bioactive polyphenolic compounds as hydroxytyrosol (HTyr) and oleuropein (OLE), known for their antioxidant, anti-inflammatory, and neuroprotective properties. This review is focused on brain, skeletal muscle, and gut microbiota, as these systems are known to interact at several levels. After the description of the chemistry and pharmacokinetics of HTyr and OLE, we summarize studies reporting their effects in in vivo and in vitro models of neurodegenerative diseases of the central/peripheral nervous system, adult neurogenesis and depression, senescence and lifespan, and age-related skeletal muscle disorders, as well as their impact on the composition of the gut microbiota.

Helichrysum italicum: From Extraction, Distillation, and Encapsulation Techniques to Beneficial Health Effects.

Helichrysum italicum (family Asteraceae), due to its various beneficial health effects, represents an important plant in the traditional medicine of Mediterranean countries. Currently, there is a renewed interest in this medicinal plant, especially in investigations involving the isolation and identification of its bioactive compounds from extracts and essential oils, as well as in experimental validation of their pharmacological activities. In this paper, we review the current knowledge on the beneficial health effects of Helichrysum italicum extracts, essential oils, and their major bioactive polyphenolic compounds, ranging from antioxidative, anti-inflammatory, and anticarcinogenic activities to their antiviral, antimicrobial, insecticidal, and antiparasitic effects. This review also provides an overview of the most promising extraction and distillation techniques for obtaining high-quality extracts and essential oils from Helichrysum italicum, as well as methods for determining their antioxidative, antimicrobial, anti-inflammatory, and anticarcinogenic activities. Finally, new ideas for in silico studies of molecular mechanisms of bioactive polyphenols from Helichrysum italicum, together with novel suggestions for their improved bioavailability through diverse encapsulation techniques, are introduced.

Antioxidative and Cytoprotective Efficacy of Ethanolic Extracted Cranberry Pomace against Salmonella Enteritidis Infection in Chicken Liver Cells

Salmonella enterica serovar Enteritidis is a globally significant zoonotic foodborne pathogen. Chicken liver is a vital organ that has been recently implicated in several reported human salmonellosis outbreaks in the U.S. One promising strategy for reducing Salmonella in chickens could be through supplementation with natural antimicrobial additives. Ethanolic extracted cranberry pomace (CPOH) is an excellent source of bioactive polyphenolic compounds with antioxidant and antimicrobial activities. However, the protective effect of CPOH against S. Enteritidis-induced chicken hepatic cell damage remains unclear. In this study, we used a chicken hepatoma cell (LMH) infection model to investigate the protective effects and potential mechanisms of CPOH. CPOH increased the viability of S. Enteritidis-infected LMH cells. Furthermore, CPOH reduced the adhesion and invasion of S. Enteritidis to LMH cells. CPOH downregulated the expression of Rho GTPase genes that are essential for Salmonella's entry into LMH cells. Additionally, the expression of antioxidant regulatory genes, such as Nrf2, HO-1, Txn, and Gclc, was increased. Our data show that CPOH effectively protected LMH cells from cell damage through the inhibition of S. Enteritidis adhesion and invasion, as well as the induction of the expression of master antioxidant genes. These findings offer opportunities to develop sustainable, safe, and economic strategies to reduce the colonization and pathogenesis of Salmonella.

The Effects of Oven Dehydration on Bioactive Compounds, Antioxidant Activity, Fatty Acids and Mineral Contents of Strawberry Tree Fruit

In this study, the effects of oven dehydration on chemical and bioactive properties, fatty acids, polyphenolic compounds and minerals of sandal strawberry tree fruit were investigated. While total carotenoid contents of the sandal strawberry tree fruit are determined between 4.20 (120 °C) and 5.43 µg/g (70 °C), tannin amounts of the sandal strawberry tree fruit were recorded between 5.13 (control) and 6.37% (70 and 120 °C). While total phenolic contents of dehydrated sandal strawberry tree fruit were found between 444.16 (120 °C) and 665.13 mgGAE/100 g (control), total flavonoid amounts of dehydrated sandal strawberry tree fruit were recorded between 592.91 (control) and 788.71 mg/100 g (120 °C). Antioxidant activity values of fruit ranged from 4.10 (120 °C) to 7.30 mmol TE/kg (control). Both total phenolic amounts and antioxidant activity values of untreated (control) sandal strawberry tree fruit were found to be higher than dehydrated ones, and a linear relationship was determined between the total phenolic amounts of the samples and their antioxidant activities. The highest amounts of phenolic compounds (ferulic acid, resveratrol and kaempferol) were detected in strawberry tree fruit dehydrated at 70 °C, followed by the control group and fruit dehydrated at 120 °C in decreasing order. Gallic acid, 3,4-dihydroxybenzoic acid, catechin, caffeic acid and rutin were the main constituents of the strawberry tree fruit, followed by syringic acid, p-coumaric acid and ferulic acid in descending order. Palmitic, stearic and oleic acid amounts of dehydrated strawberry tree fruit oils compared to the control were observed to increase with the applied temperature, while the contents of polyunsaturated fatty acids (linoleic and linolenic) decreased. In general, the mineral content of dehydrated strawberry tree fruit increased compared to the control. Since the oil, carotenoid, total phenol and phenolic component contents of sandalwood tree fruit are higher in the sample subjected to dehydration at 70 °C, this temperature can be considered as the ideal one for drying. In addition, considering the fatty acids, heat treatment at 120 °C can be preferred.

Redox Biomarkers Assessment after Oral Administration of Wine Extract and Grape Stem Extract in Rats and Mice

Wine and by-products of the winemaking process, such as grape stems, are rich in bioactive polyphenolic compounds that might be beneficial for animal and human health. In recent years, the administration of dietary polyphenols with strong antioxidant and cytoprotective properties has constituted an emerging line of research interest toward disease prevention. However, in scientific literature, only a limited number of studies have investigated the safety and the toxicological risks of polyphenolic compounds in vivo. Based on the above, the purpose of the present study was two-fold: first, to examine the effects of oral administration of a grape stem extract, derived from the Greek red wine Mavrodaphne, on mice redox biomarkers; and second, to investigate the biological effects of oral administration of a wine extract, derived from the emblematic Greek red wine Xinomavro, on rats. Toward this purpose, body weight, growth rate, hematological, biochemical, and histopathological parameters, as well as a panel of redox biomarkers, were examined. According to our results, the administration of Mavrodaphne grape stem extract in mice induced alterations in redox homeostasis, preventing mice from the adverse effects of lipid peroxidation. Contrariwise, the administration of Xinomavro wine extract induced both beneficial and harmful outcomes on rat redox status determined by the examined tissue. Collectively, our study reports that the Mavrodaphne grape stem extract, a serious pollutant when disposed in environmental matrices, is an important source of bioactive polyphenolic compounds that could protect from oxidative damage and improve animal and human health. Finally, the Xinomavro wine extract exerts tissue-specific changes in redox balance, which are indicative of the complexity that characterizes the biological systems.

Neuroprotective potential of Honokiol in ICV-STZ induced neuroinflammation, Aβ (1 −4 2) and NF-kB expression in experimental model of rats

Alzheimer’s disease (AD) is a most common and prevalent age related insidious neurological condition characterised by formation of Aβ (1−42) plaques and NFT in the hippocampus. Major symptoms of AD include memory and cognitive loss caused by neuroinflammation, Aβ (1−42) plaques, and NFT accumulation in the brain. Intracerebroventricular administration of STZ up-regulates the level of Aβ (1−42) plaques, and NFT by activating NF-kB pathway. All animals were trained for behaviour analysis before infusion of ICV-STZ bilaterally, at a dose of 3 mg/kg; icv. The stereotaxic surgery was performed in target region with coordinates −2 mm [AP], 1.6 mm [MR], and1.5 mm [DV]. On day 1 and day 3 after surgery HS (hamilton syringe) was used to infuse STZ at the target region of brain. Morris Water Maze (MWM), and Elevated Plus Maze (EPM) tests were performed to check spatial and learning memory in all groups. ICV-STZ infusion produced memory impairment by increasing the activity of AchE, oxidative markers (LPO, GSH, and nitrite), neurotransmitters levels (AchE, GABA, and glutamate), and release of neuroinflammatory markers (NF-kB, IL-6, and IL-1β). The treatment with Honokiol (Bioactive polyphenolic compound) significantly ameliorated the behavioural changes at a dose of 5, and 10 mg/kg; i.p on day 7, 14, and 21. After behavioural analysis rats were sacrificed on day 22, and the hippocampus tissue was collected to investigate the biochemical, neuroinflammatory, neurotransmitters, histopathological, and immunohistopathological changes. Here, we have found Honokiol significantly down regulates the Aβ plaques via NF-kB inhibition and also reduced neuroinflammation in the brain of rats. Further inhibits the NF-kB expression confirmed for immunohistochemistry analysis. It was observed that Honokiol (5, and 10 mg/kg; i.p) dose-dependently ameliorated AchE level restored neurotransmitters concentrations in hippocampal region, and prevented neuronal loss confirmed from histopathology studies. We concluded that Honokiol drastically produced protective effect in AD model via antioxidant, reducing inflammation, AchE level, restoration of neurofibrillary tangles and preventing NF-kB as well as histopathological changes.

Chemical Composition, Antioxidant Activity and Cytocompatibility of Polyphenolic Compounds Extracted from Food Industry Apple Waste: Potential in Biomedical Application.

Apple pomace (AP) from the food industry is a mixture of different fractions containing bioactive polyphenolic compounds. This study provides a systematic approach toward the recovery and evaluation of the physiochemical and biological properties of polyphenolic compounds from AP. We studied subcritical water extraction (SCW) and solvent extraction with ethanol from four different AP fractions of pulp, peel, seed, core, and stem (A), peel (B), seed and core (C), and pulp and peel (D). The subcritical water method at the optimum condition resulted in total polyphenolic compounds (TPC) of 39.08 ± 1.10 mg GAE per g of AP on a dry basis compared to the ethanol extraction with TPC content of 10.78 ± 0.94 mg GAE/g db. Phloridzin, chlorogenic acid, and quercetin were the main identified polyphenolics in the AP fractions using HPLC. DPPH radical scavenging activity of fraction B and subcritical water (SW) extracts showed comparable activity to ascorbic acid while all ethanolic extracts were cytocompatible toward human fibroblast (3T3-L1) and salivary gland acinar cells (NS-SV-AC). Our results indicated that AP is a rich source of polyphenolics with the potential for biomedical applications.

Current Insights on Bioactive Molecules, Antioxidant, Anti-Inflammatory, and Other Pharmacological Activities of Cinnamomum camphora Linn.

C. camphora is a renowned traditional Unani medicinal herb and belongs to the family Lauraceae. It has therapeutic applications in various ailments and prophylactic properties to prevent flu-like epidemic symptoms and COVID-19. This comprehensive appraisal is to familiarize the reader with the traditional, broad applications of camphor both in Unani and modern medicine and its effects on bioactive molecules. Electronic databases such as Web of Science, PubMed, Google Scholar, Scopus, and Research Gate were searched for bioactive molecules, and preclinical/clinical research and including 59 research and review papers up to 2022 were retrieved. Additionally, 21 classical Unani and English herbal pharmacopeia books with ethnomedicinal properties and therapeutic applications were explored. Oxidative stress significantly impacts aging, obesity, diabetes mellitus, depression, and neurodegenerative diseases. The polyphenolic bioactive compounds such as linalool, borneol, and nerolidol of C. camphora have antioxidant activity and have the potential to remove free radicals. Its other major bioactive molecules are camphor, cineole, limelol, safrole, limonene, alpha-pinene, and cineole with anti-inflammatory, antibacterial, anxiolytic, analgesic, immunomodulatory, antihyperlipidemic, and many other pharmacological properties have been established in vitro or in vivo preclinical research. Natural bioactive molecules and their mechanisms of action and applications in diseases have been highlighted, with future prospects, gaps, and priorities that need to be addressed.

Bioactive polyphenolic compounds, water-soluble vitamins, in vitro anti-inflammatory, anti-diabetic and free radical scavenging properties of underutilized alternate crop Amaranthus spinosus L. from Gangetic plain of West Bengal

Studies associated with validation of folklore and ethnomedicinal claim of utilization of different plant parts of ‘spiny amaranth’ (Amaranthus spinosus L.) against degenerative diseases with chemical data, particularly the bioactive polyphenolic compounds and associated anti-degenerative properties are scanty and is the purpose of the present investigations. RP-HPLC coupled photodiode assay was employed for the quantification of twenty-one health promoting bioactive polyphenolic antioxidants derived from phenylpropanoid pathway from different plant parts of Amaranthus spinosus L. for validating organoleptic traditional claims as well as current trends in practice of this underutilized medicinal herb. Further, important water-soluble vitamins [vitamin B1, B2, B3, B6, B9] present in different plant parts were extracted and estimated through RP-HPLC for validating the claim of their functional food properties. The antioxidant-based functional food property of different plant parts of Amaranthus spinosus were corroborated from the data of in vitro free radical scavenging, metal-chelating, reducing, superoxide radical scavenging, hydroxyl radical scavenging, anti-protein coagulation, anti-lipid peroxidation and antidiabetic properties. When compared, a positive correlation in accumulation of polyphenolic compounds and water-soluble vitamins tested with antioxidant attributes were noticed for different plant parts, strongly substantiating their phytonutrient properties. Taken as a whole, the present work provides data on organ-specific bioavailability of bioactive polyphenolic antioxidant compounds, water soluble B- vitamins and their functional attributes for validating traditional claims as well as existing trends in consumption of this underutilized medicinal vegetable as low-cost natural antioxidant supplement and functional food, which may encourage their effective exploration and utilization.

Polyphenols in Different Plant Parts of Inula grandiflora Collected from Two Habitats of Uttarakhand Himalayas

ABSTRACT The phytoconstituent analyses have been undertaken for the first time from the different plant parts of Inula grandiflora Willd. collected from two habitats (open dry slopes and moist places) of Govind Pashu Vihar National Park and Wildlife Sanctuary, Uttarakhand, India. The total phenolic and flavonoid contents and antioxidant activity have been estimated from the methanolic extracts. Ten phenols and five flavonoids were quantified in the different parts using High Performance Liquid Chromatography-Photodiode Array Detection (HPLC-PDA) method. Among the plant parts, the trend of accumulation of total phenols and flavonoids was as follows: roots > flowers > stems > leaves in both populations. It has been observed that Kalkati Dhar germplasm has the highest phenolic content in the roots (16.42 ± 0.12 mg g−1 DW), while accession collected from Renugaad has the highest flavonoid content in the flowers (4.34 ± 0.024 mg g−1 DW). Eight bioactive compounds (caffeic acid, resorcinol, p-coumaric acid, ferulic acid, veratric acid, myristicin, coumarin, and quercetin) were found dominant in the Kalkati Dhar germplasm (3200 m asl, high altitude). Significantly, seven compounds, viz; vanillic acid, vanillin, cinnamic acid, epicatechin, catechol, luteolin, and rutin, were found predominate in the Renugaad germplasm (2750 m asl, low altitude). Overall, root part accumulates most of the bioactive polyphenolic compounds as compared to the aerial parts; therefore, it has the highest antioxidant potential.

The Potential Role of Bioactive Plant-Based Polyphenolic Compounds and Their Delivery Systems—as a Promising Opportunity for a New Therapeutic Solution for Acute and Chronic Wound Healing

The Potential Role of Bioactive Plant-Based Polyphenolic Compounds and Their Delivery Systems—as a Promising Opportunity for a New Therapeutic Solution for Acute and Chronic Wound Healing

Perilla frutescens: A Rich Source of Pharmacological Active Compounds.

Perilla frutescens (L.) Britton, an important pharmaceutical and nutraceutical crop, is widely cultivated in East Asian countries. In this review, we present the latest research findings on the phytochemistry and pharmacological activities of P. frutescens. Different databases, including PubMed, Scopus, CNKI, Agricola, Scifinder, Embase, ScienceDirect, DOAJ, and Web of Science, were searched to present the best review. In this review, we clearly represent the active constituents responsible for each and every pharmacological activity, plausible mechanism of action, and maximum inhibitory concentrations, as well as IC50 values. Approximately 400 different bioactive compounds, including alkaloids, terpenoids, quinines, phenylpropanoids, polyphenolic compounds, flavonoids, coumarins, anthocyanins, carotenoids, neolignans, fatty acids, polycosanols, tocopherols, and sitosterols, have been reported in the leaves, seeds, roots, and aerial parts of P. frutescens. The bioactive constituents of P. frutescens exhibited different enzyme-inhibition properties, including antihyaluronidase effects and aldose reductase inhibitory, α-glucosidase inhibitory, xanthine oxidase inhibitory, and tyrosinase inhibitory properties. P. frutescens showed strong anti-inflammatory, antidepressant, anti-spasmodic, anticancer, antioxidant, antimicrobial, insecticidal, neuroprotective, and hepatoprotective effects. Hence, the active constituents of P. frutescens used in the treatment of diabetes and diabetic complications (retinopathy, neuropathy, and nephropathy), prevention of hyperuricemia in gout patients, hyper pigmentation, allergic conditions, skin inflammation, skin allergy, atopic dermatitis, periodontosis, androgenic alopecia, gastric inflammation, oesophagitis, carcinogenesis, cardiovascular, Alzheimer’s, Parkinson’s, and cerebral ischemic disorders. Furthermore, we revealed the most active constituents and possible mechanisms of the pharmacological properties of P. frutescens.

Exploring Polyphenol Based Bioactive Antioxidants of Underutilized Herb Amaranthus Spinosus L. for Medicinal Purposes

Though Amaranthus spinosus L. or ‘spiny amaranth’ belonging to the family Amaranthaceae is widely used in folklore and for ethnomedicinal purposes, little is known about the ecotype-based bioavailability of bioactive polyphenolic compounds that could cause health benefits. Hence, this was the objective of the present study.

Boosting the Bioaccessibility of Dietary Bioactives by Delivery as Protein-Polyphenol Aggregate Particles.

Protein-polyphenol aggregate particles concurrently fortify a functional food product with healthy dietary proteins and concentrated polyphenols. However, what impact does ingestion of aggregate particles have on ultimate health relevance of either the polyphenolic molecules in the matrix or the protein molecules? Because human health benefits are contingent on bioavailability after ingestion, the fate of these molecules during transit in the gastrointestinal tract (GIT) will dictate their utility as functional food ingredients. This brief review explores diverse applications of protein-polyphenol particles in the food industry and the bioaccessibility of both bioactive polyphenolic compounds and edible proteins. Evidence to date suggests that complexation of phytoactive polyphenolics effectively enhances their health-relevant impacts, specifically because the phytoactives are protected in the protein matrix during transit in the GIT, allowing intact, non-degraded molecules to reach the colon for catabolism at the gut microbiome level, a prerequisite to realize the health benefits of these active compounds.

UHPLC-MS Characterization, and Antioxidant and Nutritional Analysis of Cocoa Waste Flours from the Peruvian Amazon.

Cocoa (Theobroma cacao) is a food product used worldwide and a key raw material for chocolate manufacturing. Cocoa possesses bioactive compounds such as methylxanthines, flavonoids, procyanidins, and related molecules with medicinal or health-promoting properties. Cocoa shell and pod husk have been proposed as a by-product with several interesting bioactivities, and the gummy residue or glue (a sticky, gluey by-product known as “mucilage” in Spanish) is used to produce liquors and is eaten as a food in Perú. However, little is known about the chemical composition and bioactivity of flours made from Peruvian cocoa ecotype wastes such as those from the vein and pod husk of the fruits. This study aimed to characterize the in vitro antioxidant properties and nutritional values of flours made from the waste from a special ecotype of cocoa (CCN-51). The chemical fingerprinting was performed using UHPLC–HESI orbitrap mass spectrometry and allowed the detection of 51 compounds. GC-FID was used for the determination of individual fatty acid contents, and the antioxidant activity was assessed by several assays (DPPH, FRAP, and ABTS). The flours obtained were composed of a good amount of dietary fiber, carbohydrates, and minerals, as well as several bioactive polyphenolic compounds, fatty acids, and amino acids with nutraceutical properties, making the flours a rich and promising food as well as a good source for the preparation of functional foods or nutraceuticals.