Bioactive Glass Samples Research Articles

Effects of silicon nitride (Si3N4) incorporation on physicochemical, bioactivity and antibacterial properties of 45S5 bioactive glass

In this study, silicon nitride (Si3N4, SN) particles (10–30 wt%) were introduced into 45S5 bioactive glass (BG), and the impact of the incorporation on thermal and physicochemical features of the composite was analyzed. SN inclusion shifted the crystallization temperature of BG to higher temperatures, thus, the viscous flow of the glassy phase enhanced the sintering-ability and eventually reduced the sintering temperature from 1050 °C to 900 °C. XRD results revealed that increased SN fractions sustained higher amorphous content while decreasing the fraction of the main BG crystallization phase (combeite). BG + SN composites were cytocompatible with MG-63 cells. A higher number of cells was visualized on the BG + SN pellets, yet no statistically significant viability difference was determined between the BG + SN and BG samples. SN addition also endowed antibacterial activity to the BG + SN composites against S. aureus and E. coli pathogens, revealing that SN addition to BG is an alternative approach to developing biocompatible, bioactive, and antibacterial materials.

In vitro cytotoxicity of magnetic-fluorescent bioactive glasses on SaOS-2, MC3T3-E1, BJ fibroblast cells, their hemolytic activity, and sorafenib release behavior

In the study, the fabrication of superparamagnetic-fluorescent bioactive glasses in the form of the particle, nanofiber, and 3D scaffolds was performed by including maghemite (γ-Fe2O3) nanoparticles and photoluminescent rare earth element ions (Eu3+, Gd3+, and Yb3+) using sol-gel, electrospinning, and robocasting techniques, respectively. The in vitro cytotoxicity of the magnetic-fluorescent bioactive glasses on osteosarcoma SaOS-2, pre-osteoblast MC3T3-E1, and BJ fibroblast cells, as well as their hemolytic activity and sorafenib tosylate loading and release behavior, were investigated. The cytotoxicity of the bioactive glass samples was tested using the MTT assay. Additionally, the alkaline phosphatase activity of the studied glasses was examined as a function of time. The mineralization behavior of the pre-osteoblast cell-seeded glass samples was analyzed using Alizarin red S staining. Results revealed that the in vitro cytotoxicity of the studied bioactive glasses in the form of particles and nanofibers depended on the sample concentration, whereas in the case of the 3D scaffolds, no cytotoxic response was observed on the osteosarcoma, pre-osteoblast, and fibroblast cells. Similarly, particle and nanofiber-based glass samples induced dose-dependent hemolysis on red blood cells. Drug loading rates were much lower for the 3D scaffolds compared to the particle and nanofiber-based samples. Drug release rates ranged from 25 % to 90 %, depending on the bioactive glass morphology and the pH of the release medium. It was concluded that the studied bioactive glasses have the potential to be used in tissue engineering applications and cancer therapy.

Fabrication and characterization of novel multifunctional superparamagnetic and fluorescent bioactive glasses for biomedical applications

Magnetic-fluorescent nanosystems have wide applications in the biomedical field due to their dual ability of unique biomolecular fluorescent recognition and magnetic modes. In this study fabrication of superparamagnetic-fluorescent bioactive glasses in the form of particle, nanofiber, and 3D scaffold was performed by the inclusion of maghemite (γ-Fe2O3) nanoparticle and photoluminescent rare earth element ions (Eu3+, Gd3+, and Yb3+). Bioactive glasses in the form of particle, nanofiber, and 3D scaffolds were manufactured using sol-gel, electrospinning, and robocasting techniques, respectively. Morphological, structural, magnetic, and luminescence properties of the fabricated bioactive glasses have been investigated comprehensively using electron microscopy, x-ray diffractometer, vibrating sample magnetometer, Fourier transform infrared, and fluorescence spectrometers. The compressive strength of the robocast 3D glass scaffolds was measured. In vitro hydroxyapatite deposition on the surface of the bioactive glasses soaked in simulated body fluid for various times up to 28 days was also examined. Results showed that all of the bioactive glasses prepared in the study with different morphological characteristics have superparamagnetic properties. Saturation magnetization (Ms) values of the glasses were in the range of 7.20–12.50 emu/g. Additionally, all of the studied glass samples showed fluorescence behavior. The highest photoluminescence emission peak intensity was recorded for the Eu3+-doped robocast 3D glass scaffolds which is followed by nanofibrous and particulate forms of bioactive glass. Results of the in vitro bioactivity experiments showed that hydroxyapatite formation occurred on all of the bioactive glass samples and the highest hydroxyapatite formation rates were obtained for the fibrous glass samples due to their higher surface area.

Correlation between optical and shielding properties of phosphate glasses with alkaline oxide and their application

In numerous tissue engineering and dental applications, bioactive glasses are utilized. These glasses have unique characteristics that make them attractive candidates for a variety of applications. A new bioactive glass system with the structure of 45P2O5 − 20CaO − 15CaCL2 − 8KF − (10 − x) Li2O − (x) TiO2 was developed in this study, with x = 2, 6, and 8 mol%. For usage in radiation protective applications, it was evaluated. By using an ultraviolet–visible spectrophotometer, we were able to measure the absorbance (Abs) and transmittance (T %) in the range of wavelengths 190–2500 nm. Furthermore, the optical energy gap of the produced glasses was determined. Using the MIKE software, the mass attenuation coefficients (MAC) of the bioactive glasses under investigation were calculated for energies ranging from 15 to 200 keV. The 𝐿𝐿𝐿𝐿𝐿𝐿, 𝑍𝑍𝑒𝑒𝑒𝑒𝑒𝑒, 𝑁𝑁𝑒𝑒𝑒𝑒𝑒𝑒, 𝐻𝐻𝐻𝐻𝐻𝐻, 𝑇𝑇𝑇𝑇𝑇𝑇, 𝑎𝑎𝑎𝑎𝑎𝑎 𝑀𝑀𝑀𝑀𝑀𝑀 (Linear attenuation coefficient, effective atomic number, effective electron density, half value layer, tenth value layer, and mean free path) of the bioactive glasses were calculated. According to the findings, the addition of titanium dioxide (TiO2) as well as the metal oxide such as Li2O to bioactive glasses generates significant differences in the attenuation characteristics of bioactive glasses. The results indicate that the PCKLT3( 𝑇𝑇𝑇𝑇𝑇𝑇2= 8mol%) bioactive-glass sample had the best attenuation among other samples.

New and Efficient Bioactive Glass Compositions for Controlling Endodontic Pathogens.

Endodontic treatment aims to conserve teeth through removing infected tissue, disinfecting, and filling/sealing the root canal. One of the most important treatment steps is the removal of microorganisms to avoid reinfection and consequent tooth loss. Due to increased resistance to intracanal medications, new alternative procedures are needed. Thus, an intracanal medication is suggested using three bioactive glass (BG) compositions (BG1, BG2, and BG3) produced by the sol–gel method, with different molar contents of bactericidal oxides. The BGs were morphologically and physically characterized. Their ability to inhibit the growth of two oral pathogens responsible for the failure of endodontic treatments (E. faecalis and C. albicans) was also studied. The results suggest that BG2 and BG3 can inhibit the growth of E. faecalis after 48 h of incubation, and all BG samples have a significant effect on C. albicans survival.

Co-doping of iron and copper ions in nanosized bioactive glass by reactive laser fragmentation in liquids.

Bioactive glass (BG) is a frequently used biomaterial applicable in bone tissue engineering and known to be particularly effective when applied in nanoscopic dimensions. In this work, we employed the scalable reactive laser fragmentation in liquids method to produce nanosized 45S5 BG in the presence of light-absorbing Fe and Cu ions. Here, the function of the ions was twofold: (i) increasing the light absorption and thus causing a significant increase in laser fragmentation efficiency by a factor of 100 and (ii) doping the BG with bioactive metal ions up to 4wt%. Our findings reveal an effective downsizing of the BG from micrometer-sized educts into nanoparticles having average diameters of <50 nm. This goes along with successful element-specific incorporation of the metal ions into the BG, inducing co-doping of Fe and Cu ions as verified by energy-dispersive X-ray spectroscopy (EDX). In this context, the overall amorphous structure is retained, as evidenced by X-ray powder diffraction (XRD). We further demonstrate that the level of doping for both elements can be adjusted by changing the BG/ion concentration ratio during laser fragmentation. Consecutive ion release experiments using inductively-coupled plasma mass spectrometry (ICP-MS) were conducted to assess the potential bioactivity of the doped nanoscopic BG samples, and cell culture experiments using MG-63 osteoblast-like cells demonstrated their cytocompatibility. The elegant method of in situ co-doping of Fe and Cu ions during BG nanosizing may provide functionality-advanced biomaterials for future studies on angiogenesis or bone regeneration, particularly as the level of doping may be adjusted by ion concentrations and ion type in solution.

13-93B3 Bioactive glasses containing Ce3+, Ga3+ and V5+: dose rate and gamma radiation characteristic for medical purposes

In this study, dose rates and radiation attenuation features of a group of fabricated bioactive glasses were investigated. The borate-based 13-93B3 bioactive glass powders (B3) containing cerium (III), gallium (III) or vanadium (IV) were prepared by melting a homogenized mixture of reagent-grade CaCO3, Na2CO3, MgCO3, K2CO3, H3BO3, CaHPO4.2H2O, Ga2O3, V2O5 or Ce(CH3CO2)3, and disc-shaped scaffolds were prepared by die pressing. Bioactive glasses were modelled by using MCNPX (version 2.7.0) general-purpose Monte Carlo code. A gamma-ray transmission set-up was utilized for determination of mass attenuation coefficients. The obtained coefficients were used for determination of other essential attenuation properties. Finally, exposure (EBF) and energy absorption (EABF) build-up factors were determined. Although the chemical structure of the additive material in bioactive glasses is identical, it can be inferred that the chemical structure of the additive is closely linked to the radiation attenuation characteristics of the bioactive glasses. Results also revealed that, although the bulk densities of the disc-shaped bioactive glass samples were lower than the measured true density values of the melt-derived glass powders due to porosity concerns, they exhibited radiation shielding effect. Findings of the study may be useful in understanding the radiation shielding characteristics of the bioactive glass scaffolds fabricated by powder processing. It can be concluded that outcomes of recent investigation can be useful during the evaluation of potential interactions between the bioactive glasses and medical radiation in the body.

Effect of Sr on the Bioactivity of Sol-Gel Derived New Silicate Bioglass S55P4

Strontium (Sr) stimulates osteoblast and inhibits osteoclast activities in-vitro and is used clinically as a treatment for osteoporosis. In this research, the effect of Sr substitution on the apatite formation of sol-gel derived bioactive glass (BG) (55.90SiO2-1.72P2O5 -21.67Na2O - (20.69-x) CaO -x SrO) (x=0, 5 and 8 mol. %) were investigated. The synthesized Sr doped BG samples were treated in Hank's Balanced Salt Solution (HBSS) for 14 days to study the bioactivity. The achieved samples were evaluated by X-ray powder diffraction (XRD) and Scanning electron microscope (SEM). In XRD, the hydroxyapatite (HA) crystalline peak for 8% Sr-BG is less compared with others. When Sr amount is increased to 8%, the low crystalline peaks of HA were detected although the same soaking duration. FTIR spectra supported the delay precipitation of calcium phosphate (CaP), especially for the specimen containing 8% Sr. After 14 days soaking, SEM images confirmed the bioactivity of the synthesized samples by the formation of apatite on the glass surface.

X-ray scattering study of water confined in bioactive glasses: experimental and simulated pair distribution function.

Temperature-dependent total X-ray scattering measurements for water confined in bioactive glass samples with 5.9 nm pore diameter have been performed. Based on these experimental data, simulations were carried out using the Empirical Potential Structure Refinement (EPSR) code, in order to study the structural organization of the confined water in detail. The results indicate a non-homogeneous structure for water inside the pore, with three different structural organizations of water, depending on the distance from the pore surface: (i) a first layer (4 Å) of interfacial pore water that forms a strong chemical bond with the substrate, (ii) intermediate pore water forming a second layer (4-11 Å) on top of the interfacial pore water, (iii) bulk-like pore water in the centre of the pores. Analysis of the simulated site-site partial pair distribution function shows that the water-silica (Ow-Si) pair correlations occur at ∼3.75 Å. The tetrahedral network of bulk water with oxygen-oxygen (Ow-Ow) hydrogen-bonded pair correlations at ∼2.8, ∼4.1 and ∼4.5 Å is strongly distorted for the interfacial pore water while the second neighbour pair correlations are observed at ∼4.0 and ∼4.9 Å. For the interfacial pore water, an additional Ow-Ow pair correlation appears at ∼3.3 Å, which is likely caused by distortions due to the interactions of the water molecules with the silica at the pore surface.

Effects of Sr and Mg dopants on biological and mechanical properties of SiO2–CaO–P2O5 bioactive glass

In the present study, the effects of Sr and Mg were investigated on mechanical and biological properties of 58S bioactive glass (BG). SiO2-P2O5-CaO BG with different contents of Sr and Mg were synthesized via the sol-gel method and immersed in simulated body fluid (SBF) for several days to explore their biocompatibility. Precise analyses of the BG using X-ray powder diffraction, scanning electron microscopy, and transmission electron microscopy showed that the Mg-doped BG containing 8 wt % MgO possessed better biocompatibility. It was also found that mechanical properties of the BG could be improved by increasing the amounts of MgO and SrO. Both 5Sr-BG and 8Mg-BG samples did not exhibit any cytotoxicity while showing high alkaline phosphatase activity in comparison with control specimens. However, the Sr-doped BG sample including 5 wt % SrO demonstrated enhanced bioactivity and biocompatibility.

Bioactive glasses with TiO2 additive: Behavior characterization against nuclear radiation and determination of buildup factors

This paper studies the radiation attenuation properties and build up (EBF and EABF) factors of different types of bioactive glasses in the 0.015–15 MeV photon energy range. EBF and EABF values of investigated bioactive glasses are determined by using the G-P fitting method. Moreover, effective removal cross-section values, alpha/proton mass stopping power (MSP), and alpha/proton projected range values (PR) are determined for fast neutrons and charged particles. The investigation indicates that TiO2 additive in the bioactive glasses causes considerable variations in the attenuation properties of bioactive glasses. The results show that the BT2.5 bioactive glass sample has highest attenuation performance among the samples.

Electrospinning 3D bioactive glasses for wound healing

An electrospinning technique was used to produce three-dimensional (3D) bioactive glass fibrous scaffolds, in the SiO2–CaO sol-gel system, for wound healing applications. Previously, it was thought that 3D cotton wool-like structures could only be produced from sol-gel when the sol contained calcium nitrate, implying that the Ca2+ and its electronic charge had a significant effect on the structure produced. Here, fibres with a 3D appearance were also electrospun from compositions containing only silica. A polymer binding agent was added to inorganic sol-gel solutions, enabling electrospinning prior to bioactive glass network formation and the polymer was removed by calcination. While the addition of Ca2+ contributes to the 3D morphology, here we show that other factors, such as relative humidity, play an important role in producing the 3D cotton-wool-like macrostructure of the fibres. A human dermal fibroblast cell line (CD-18CO) was exposed to dissolution products of the samples. Cell proliferation and metabolic activity tests were carried out and a VEGF ELISA showed a significant increase in VEGF production in cells exposed to the bioactive glass samples compared to control in DMEM. A novel SiO2–CaO nanofibrous scaffold was created that showed tailorable physical and dissolution properties, the control and composition of these release products are important for directing desirable wound healing interactions.

Antibacterial Properties among Different Concentration of Bioactive Glasses

The objective of this study was to evaluate the antibacterial properties and pH changes of bioactive glasses and zinc oxide nanowire in different concentrations. Bioactive glasses (45S5 and 45S5F) were prepared in three concentrations of 10, 20, and 50 mg/ml and zinc oxide nanowire was prepared in 1 and 5 mg/ml concentrations. The materials were exposed to 500 ml brain heart infusion broth (BHI) with 1.5 x 107 of S.mutans and S.sanguinis separately. Antibacterial properties were tested indirectly by collecting 100 ml of each sample and transferred into a 96 well-plate. The optical density (OD) was evaluated using spectrophotometry at 630 nm at 24h and 48h. The pH changes were recorded. The data were statistically analyzed by Kruskal-Wallis tests. The result showed that the pH changes were significantly different in the Bioactive glass samples, while zinc oxide nanowire showed stable pH. Antibacterial activity against S.mutans was significant lower for 45S5 at 50 mg/ml, 45S5F and zinc oxide nanowire in all concentrations at 24 h. While in 48 h, 45S5, 45S5F and zinc oxide nanowire showed significant antibacterial activity in all concentration except 45S5F at 10 mg/ml. Antibacterial activity against S.sanguinis was significant for 45S5 and 45S5F at 20 and 50 mg/ml and zinc oxide nanowire in all concentration at 48h. It can be concluded that Bioactive glasses (45S5 and 45S5F) exhibited antibacterial properties and pH changes depending on its concentration, while zinc oxide nanowire exhibited antibacterial properties at low concentrations with a constant pH value.

Effect of Silver on the Apatite Formation of Bioactive Silicate Glass

Abstract Silver (Ag) has the antibacterial property and can be easily introduced in glasses and glass-ceramics. In this research, the effect of Ag addition on the apatite formation of sol-gel derived new composition bioactive glass (BG) (55%SiO2 - 4% P2O5 -22%Na2O - (19-x) %CaO -x% Ag2O) (x=0, 5 and 8%) were investigated. The synthesized Ag doped BG samples were treated in Hank's Balanced Salt Solution (HBSS) for 14 days to study the bioactivity. The achieved samples were evaluated by X-ray powder diffraction (XRD) and Scanning electron microscope (SEM). The XRD spectra clearly show diffraction peaks of bone-like hydroxyapatite (HAp) for all samples after immersion in HBSS. However, the more intense of HAp peak at 32° was detected in Ag(5%) and Ag(8%) BGs compared with undoped BG. All glass samples are bioactive but BG with 8 wt% of Ag2O exhibited more apatite than the samples containing 5 wt% of Ag2O, as seen in SEM images. After 14 days immersion, full coverage of apatite in cluster crystal form on the Ag(8) glass surface was observed.

Enhanced in vivo biocompatibility of magnesia-contained bioactive glasses

Human blood compatibility of previously prepared magnesia-doped bioactive glass samples was evaluated by hemolysis assay. The in vivo evaluation was carried out using a rat model in which a hole was made in the rat femur bone by drilling and the bioactive glass samples were implanted in the cavity. Histological examination had demonstrated the better bone regeneration and blood vessel formation as compared to the reference one. The in vivo complete blood count (CBC) was also analyzed at different time periods. The in vivo results of magnesium-containing bioactive glasses had shown improved bioactivity as well as better biocompatibility.

Femtosecond laser induced surface modification for prevention of bacterial adhesion on 45S5 bioactive glass

Bacterial attachment and biofilm formation on implant surface has been a major concern in hospital and industrial environment. Prevention of bacterial infections of implant surface through surface treatment could be a potential solution and hence this has become a key area of research. In the present study, the antibacterial and biocompatible properties of femtosecond laser surface treated 45S5 bioactive glass (BG) have been investigated. Adhesion and sustainability of both gram positive S. aureus and gram negative P. aeruginosa and E. coli nosocomial bacteria on untreated and laser treated BG samples has been explored. An imprint method has been used to visualize the growth of bacteria on the sample surface. We observed complete bacterial rejection potentially reducing risk of biofilm formation on laser treated surface. This was correlated with surface roughness, wettability and change in surface chemical composition of the samples before and after laser treatment. Biocompatibility of the laser treated BG was demonstrated by studying the anchoring and growth of human cervix cell line INT407. Our results demonstrate that, laser surface modification of BG enables enhanced bacterial rejection without affecting its biocompatibility towards growth of human cells on it. These results open a significantly potential approach towards use of laser in successfully imparting desirable characteristics to BG based bio-implants and devices.

Re-crystallization of silica-based calcium phosphate glass prepared by sol–gel technique

Abstract Bioactive glass (BG) is a potential material for treating dentin hypersensitivity owing to its high solubility. In this study, we synthesized 80S-BG bioactive glass samples using a sol–gel technique and mixed with various hardening agents. The obtained material could be used in human dentinal dentinal tubule occlusions. X-ray diffraction (XRD), scanning electronic microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) measurements were employed to investigate the physiochemical properties and dentinal dentinal tubule occlusion efficiency by mixing the 80S bioactive glass (80S-BG) with various hardening agents. The major crystallite phase obtained on mixing 80S-BG with phosphoric acid (PA) was Ca(H 2 PO 4 ) 2 ·H 2 O. The mixture of 80S-BG powders and 20, 30, or 40 wt% PA acted as a hardening agent and achieved a dentinal tubule penetration depth of 30.7–62.6 µm. 80S-BG on mixing with suitable PA agents exhibited a short reaction time and good operability, making it feasible for use in occluding dentinal tubules. 80S-BG mixed with hardening agents exhibited a greater potential for treating dentin hypersensitivity as compared to the 80S-BG not mixed with any hardening agents.

Characterization of Some Bioactive Glasses and Glass-ceramics Prepared by a Hydrothermal Method

Powders of different samples of bioactive silicate glass ceramics were prepared by a hydrothermal method (HTM). The thermal energy assisted method was applied as an alternative to ultrasonic and microwave energy radiation assisted tools. Characterization of the glasses was carried out by FTIR (Fourier transform infrared) absorption spectroscopy. The different crystalline phases and crystallographic parameters were explored using X-ray diffraction (XRD) analysis. Differential scanning calorimetry and thermogravimetric analysis were carried out to investigate the thermal characteristics of the prepared samples. Transmission electron microscopy (TEM) and electron diffraction patterns (EDP) were performed to examine the internal microstructure of the bioactive glass samples. The hydrothermal assisted method is an appropriate technique to carry out the reaction in a short time. In addition, this method can modify the reaction environment to produce amorphous glassy materials after just a short time of thermal treatment. The sintered glass-ceramic materials prepared by such technique are characterized with fine size and more crystallized structure than those prepared by the conventional melting method. Fine structure leads to an increase in surface area which in turn participates to a great extent in improving compatibility and bioactivity of the materials.

Characterization of biomaterials commonly used in dentistry for bone augmentation by X-ray and electron techniques

Hydroxyapatite (HA), beta-tricalcium phosphate and bioactive glasses are commonly used as reabsorbable biomaterials, mainly in orthopaedics and dentistry. The performance of each material depends on many factors, in particular, on their chemical and phase composition, microstructure, granule size and pore volume. For this reason, it is important to have a full characterization that allows correlating these properties with the material biological behaviour. In this work, three commercial samples of materials currently used in dentistry as bone substitutes were characterized. Granules corresponding to bovine and synthetic HA and bioactive glass 45S5 type were studied by scanning electron microscopy, conventional and synchrotron radiation X-ray diffraction and X-ray fluorescence. The specific surface area was also obtained by the Brunauer, Emmett and Teller method. We observed that Ca/P molar ratios for both HAs are higher than the value corresponding to the stoichiometric HA. The coherent domain obtained for the bovine HA is larger along the c axis crystal direction, and it is around 15 times lower than the value corresponding to the synthetic HA. The specific surface area for the bovine HA is one of the highest values reported in literature. Low amounts of crystalline CaO were observed only for the synthetic HA sample. Crystalline combeite and wollastonite were detected for the bioactive glass sample and quantified by using rutile as internal standard. The relation between the physico-chemical characterization performed in this work and the potential biological response of the materials is discussed in terms of the information available in literature. Copyright © 2016 John Wiley & Sons, Ltd.

Bactericidal Activity of Copper Oxide Nanocomposite/Bioglass for in Vitro Clindamycin Release in Implant Infections Due to Staphylococcus aureus

Background: In recent years, bioactive bioceramics such as bioglass and hydroxyapatite (HA) have been introduced as a remarkable development in the field of medicine due to their bio-adaptability, non-toxicity, and persistence, in vivo. They have many potential applications in the repair of bone defects and hence they have attracted significant interest from scholars. Objectives: The aim of this study was to synthesize inorganic matrix CuO-based bioglasses and evaluate their antibacterial activity against aerobic bacterial infections in bone implants. Methods: Nano-composite samples of silica-based bioactive glass, 60SBGwith nano-powderCuO, were synthesized using the sol-gel method and then assessed with regard to their antibacterial properties against Staphylococcus aureus using well diffusion agar. The samples included BG58S (58%SiO2, 36%CaO, 6%P2O5), BG/10CuO (58%SiO2, 26%CaO, 6%P2O5, 10%CuO), and BG/20CuO (48%SiO2, 26%CaO, 6%P2O5, 20%CuO). To evaluate their bioactivity, the prepared samples of BG/20CuO, BG/10CuO, and BG58S were immersed in simulated body fluids (SBF). The surface morphology and structure of the samples before and after immersion in the SBF were characterized using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR), respectively. Then, the BG/20CuO and BG/10CuO samples were loaded in clindamycin, an antibiotic widely used in the treatment of osteomyelitis, and their release profiles were studied in phosphate buffer solution. Results: It was observed that the growth inhibition zone increased through clindamycin release due to the increasing CuO percentage in the nanocomposite of bioactive glass. The bioactivity of the nanocomposite/bioglass with CuO was superior to that of bioglass alone. In this study, the BG/20CuO sample showed a sustained release of clindamycin, which is sufficient for a drug delivery system. Conclusions: Increasing the Cu nanoparticles in bioactive glass samples leads to the release of Cu2+, which has a positive effect on the antibacterial mechanism, as well as decreasing the cultured Staphylococcus colonies found on the bioglasses. Therefore, it seems that the nanocomposite/bioglass of CuO is a promising option for aerobic bacterial inhibitor systems in common bone implant infections.