IntroductionGreen nanosynthesis of medicinal plants are useful for the development of antibiotic agents. In this work, we have explored the antimicrobial and antioxidant activity of green synthesized CuO NPs from tropical medicinal plant Ipomoea quamoclit. L. MethodsWith the aid of microfluidic technology, a low-cost, PDMS lab-on-chip micromixer for green nanosynthesis of Copper Oxide (CuO) nanoparticle from Ipomoea is presented. The UV- Vis characteristics, FTIR spectrum, antimicrobial and antioxidant response using DPPH and H2O2 were reported. The antimicrobial efficacy of the synthesized CuO-NPs is tested against two-gram positive bacteria Staphylococcus aureus, Micrococcus luteus, two gram negative bacteria, Enterobacter cloacae, Klebsiella pneumoniae and two fungi species Candida albicans, Aspergillus niger. ResultsUV-Vis spectrum for the synthesized CuO-NPs extract showed the absorbance at 670 nm, SEM image analysis and DLS analysis confirmed the presence of spherical sized nanoparticle between 65 and 94.5 nm. The presence of bioactive functional groups of N-H, C-H and C=0 stretching was identified using Fourier Transform Infrared spectroscopy (FTIR).The maximum mean zone of inhibition obtained was 10.45±0.25mm for fungi Aspergillus niger. The antioxidant activity using DPPH scavenging assay and Hydrogen peroxide scavenging assay showed the IC50 value 33.94 μg/ml and42.54μg/ml respectively. ConclusionsThe obtained results reveal the potential of CuO NPs synthesised from Ipomoea quamoclit. L. as a remarkable medicinal entity for wound healing, respiration, fungal infection and urinary tract infection along with the improved antimicrobial and antioxidant activity.