Chemical Profiling, Spectroscopic Characterization and Biological Evaluation of a novel polyherbal formulation with natural binders- Ayurgreen Natura Pain Gel

Abstract

Introduction: Herbal compositions are becoming increasingly important in today's world of raw material scarcity. Polyherbal formulations exhibit high efficacy due to the presence of active phytochemicals which may enhance their potency due to the synergetic interaction of active ingredients of different plants. Ayurgreen Natura Pain Gel is an important Ayurvedic polyherbal formulation prepared using specified plant parts of dried aloe vera and fresh aloe vera pulp, frankincense, myrrh, ferula asafetida. Methods: The phytochemistry of Ayurgreen Natura Pain Gel has been evaluated using a liquid chromatography-mass spectrometer and their bioactive functional groups were characterized using Fourier Transform Infrared Spectroscopy and UV-Visible spectroscopy. Moreover, the thermal analysis was performed using differential scanning calorimetry. Further, invitro studies were used to evaluate its anti-inflammatory, antioxidant and anti-cancerous activities. Results: The LCMS results revealed the presence of 40 phytoconstituents. It shows the presence of Manumycin A which helps wound healing by binding it with RAS protein. The thermogram results revealed the presence of volatile ingredients, melting, and degradation temperature. The formulation showed remarkable anti-inflammatory (IC50 119.8 μg/mL) and antioxidant (IC50 200 μg/mL) activities. The formulation showed potent cytotoxic effect towards Ehrlich ascites carcinoma (EAC) and Dalton’s lymphoma ascites (DLA) cell lines with IC 50 values 62 mg/mL and 20 mg/mL respectively. Discussion/Conclusions: The formulation can be considered as a potent anti-inflammatory cum anti-cancerous Natura Pain Gel with antiproliferative activity. Fascinatingly, the wild habitat contained some anticancerous phytoconstituents which might be responsible for enhanced anti-cancerous activity in mice cancer cell lines (EAC) and (DLA) cell lines.