Release Of Bioactive Compounds Research Articles

Enhanced in vitro toxicity of plastic leachates after UV irradiation

Plastics can release numerous chemicals and thereby, contribute to the chemical pollution in aquatic systems. To which extent environmental degradation processes influence the release of plastic chemicals, is currently unknown and subject of research. We therefore evaluated aqueous leachates of 12 differently formulated plastics (e.g., pre-production, post-industrial and recycled pellets as well as final products) using in vitro bioassays and chemical analysis via LC-HRMS nontarget approach. We weathered these plastics by UV irradiation (UV-C and UV-A/B) under laboratory conditions in dryness and a subsequent leaching period in ultrapure water (‘atmospheric’ weathering) or directly in water (‘aquatic’ weathering, UV-A/Baq). A dark control (DC) without UV light served as a reference treatment. Some plastics triggered several toxicological endpoints (low-density polyethylene recyclate (LDPE-R), starch blend (SB), bio-based polybutylene succinate (Bio-PBS) and polyvinyl chloride (PVC)), whereas others caused little to no effects (polyethylene terephthalate (PET), polystyrene (PS), polypropylene (PP) and LDPE). UV irradiation enhanced the plastics’ toxicity, even for samples initially evaluated as toxicologically inconspicuous. The plastic samples caused oxidative stress (85%), baseline toxicity (42%), antiestrogenicity (40%) and antiandrogenicity (27%). Positive findings were measured after UV-C (63%) and UV-A/Baq (50%) treatments, followed by UV-A/B (48%) and DC (33%). Overall, we detected between 42 (DC) and 2896 (UV-A/Baq) chemical compounds. Our study demonstrates that differently formulated plastics leach toxic chemicals. UV exacerbates the plastics’ toxicity by either generating active compounds and/or by facilitating their release. UV light even leads to the release of bioactive compounds from plastics of low chemical complexity. To prevent the exposure to plastic-associated chemicals, the application of chemicals could be reduced to a minimum, while on a regulatory level the evaluation of plastic eluates could be another focal point next to singular compounds.

Microcapsules of bioactive compounds from fruits and vegetables waste and their utilization: A review

Fruits and vegetables have crucial role in human nutrition. Due to presence of various bioactive compounds, they are consumed processed, minimally processed as well as raw. In fruit and vegetable processing industries, the generation of their wastes are becoming a serious nutritional and environmental issue. These wastes still contain many bioactive compounds and their recovery from industry wastes has received significant attention. These bioactive compounds are sensitive to heat, light and oxygen; therefore, entrapment of these bioactive compounds is necessary to provide them protection from degradation against the environmental conditions. Additionally, microencapsulation process has been used for improving bioavailability, stability, delivery and controlled release of bioactive compounds. The aim of this review is to compile the results of extraction of different bioactive compounds by different methodologies and then their incorporation into probiotics in order to develop a functional food product with improved health and nutritional characteristics.

Nanophytosome as a promising carrier for improving cumin essential oil properties

The health benefits of nanoparticles in preservation of sensitive compounds such as essential oil (EO) to overcome its application difficulties, e.g., instability, low water solubility, and oral bioavailability. In this study, CEO-loaded nanophytosomes (NP) were prepared using a thin layer hydration technique in different ratios of CEO to phosphatidylcholine (PC). Colloidal properties of NP such as particle size, ζ-potential, polydispersity index (PDI), encapsulation efficiency (EE), CEO-PC ionic interactions, antioxidant activity, turbidity, stability and release behavior were investigated. CEO-NP showed good characteristics, i.e., d<100 nm particle sizes, low size distribution (PDI<0.3), EE>90% and relatively uniform size (unchanged size and turbidity during one month of storage). The ζ-potential changed from +9 mV in the blank (B) NP to -19 mV in encapsulated EO. EO-encapsulated in NP showed a relative constant release pattern. About 88% of free CEO remained stable in simulated gastric fluid at pH 2 while 95% was eliminated during 2.5 h in simulated intestinal fluid at pH 7. However, NP improved the stability of CEO 6.7 times in SIF. This study indicated that the release of bioactive compounds from NP can be efficiently reduced using encapsulation into NP, and offered CEO-loaded NP as an efficient candidate for food and beverage fortification to produce functional foods.

A Theoretical Model for Release Dynamics of an Antifungal Agent Covalently Bonded to the Chitosan

The aim of the study was to create a mathematical model useful for monitoring the release of bioactive aldehydes covalently bonded to the chitosan by reversible imine linkage, considered as a polymer–drug system. For this purpose, two hydrogels were prepared by the acid condensation reaction of chitosan with the antifungal 2-formyl-phenyl-boronic acid and their particularities; influencing the release of the antifungal aldehyde by shifting the imination equilibrium to the reagents was considered, i.e., the supramolecular nature of the hydrogels was highlighted by polarized light microscopy, while scanning electron microscopy showed their microporous morphology. Furthermore, the in vitro fungicidal activity was investigated on two fungal strains and the in vitro release curves of the antifungal aldehyde triggered by the pH stimulus were drawn. The theoretical model was developed starting from the hypothesis that the imine-chitosan system, both structurally and functionally, can be assimilated, from a mathematical point of view, with a multifractal object, and its dynamics were analyzed in the framework of the Scale Relativity Theory. Thus, through Riccati-type gauges, two synchronous dynamics, one in the scale space, associated with the fungicidal activity, and the other in the usual space, associated with the antifungal aldehyde release, become operational. Their synchronicity, reducible to the isomorphism of two SL(2R)-type groups, implies, by means of its joint invariant functions, bioactive aldehyde compound release dynamics in the form of “kink–antikink pairs” dynamics of a multifractal type. Finally, the theoretical model was validated through the experimental data.

Whey protein based electrosprayed nanospheres for encapsulation and controlled release of bioactive compounds from Tinospora cordifolia extract

The synergistic effects of bioactive compounds of Tinospora cordifolia have insulin mimicking and hypoglycemic activity, however, low bioavailability and poor stability limits its potential. In the present study, an appropriate delivery system was developed for the controlled release of its anti-diabetic activity. The bioactive compounds such as palmatine, berberine and palmatoside had better binding energy as observed in docking studies compared to that of the commercial active compounds. However, as these biocompounds from Tinospora cordifolia are associated with low stability and poor bioavailability, these compounds were encapsulated in a core-shell matrix of whey protein isolate. The bioactive compounds had highest antidiabetic activity in chloroform extract with an IC50 concentration of 11.34 mg/ml. An increase in 28.12% activity was observed in nanoemulsion form with an average size of 82.68 ± 4.37 nm. The bioactive compounds were further encapsulated by electrospray technique for increased stability. The particles had an encapsulation efficiency of 91.2 ± 3.27% with an average particle size of 187 ± 2.71 nm. The kinetic study revealed the complete release of bioactive compounds after 24 h of incubation in buffer solution. This formulation can be further explored as novel nutraceutical delivery system with minimal side effects as compared to their synthetic counterparts. Considering the potential application of this developed technology, further upscaling as well as in-vivo experimentation on small as well as large animals should be performed.

The Prospection of Plant Response to 2-Ketones Released from Nanostructured Lipid Carriers

Nanotechnology plays an important role to increase food production by improving plant performance. Specifically, nanostructured lipid carriers (NLCs) are an important tool for the controlled release of compounds in agriculture. Furthermore, volatile organic compounds (VOCs) have shown an important growth-inducing effect in vegetables. Nevertheless, there is no tool designed for VOC application in agricultural systems. Therefore, the study proposes the use of NLC to the controlled release of VOC to apply in horticulture. The focus of this study was to evaluate the direct impact of 2-ketones released from NLC on Solanum lycopersicum cv Marglobe and Lactuca sativa cv Reina de Mayo. NLCs synthesized with glyceryl tristearate and isopropyl myristate were characterized and used to encapsulate 250 ppm of 2-nonanone, 2-undecanone, or 2-tridecanone. Then, seedlings of S. lycopersicum and L. sativa were exposed to 2-ketones released from NLC for 10 days. Electrolyte leakage, anthocyanin, mass percentage, stomatal aperture, and chlorophyll fluorescence were measured. The data indicated that NLC had a mean size of 205 nm and homogeneity. NLC showed a capacity for the controlled release of 2-ketones, depending on the carbon number of the encapsulated compound. The release of 2-ketones did not have a significant effect on electrolyte leakage and anthocyanin content, but they modulate the percentage of surface elements. The role of 2-ketones to increase stomata aperture and chlorophyll concentration is noted. The results strongly suggest that NLCs are potential carriers for the release of bioactive compounds with potential use in agriculture.

Preparation and characterization of soybean protein isolate/pectin-based phytosterol nanodispersions and their stability in simulated digestion

In this study, stigmasterol was nanoencapsulated in soy protein isolate -pectin-based nanodispersions. Based on the particle size and zeta-potential, the optimal pectin/SPI ratio of stigmasterol nanodispersion was determined to be 1:10. At this ratio, nanodispersions was manufactured with an average particle size of 477 ± 33 nm, an encapsulation efficiency of 89.37%, and a loading amount of 17.87%. The physical properties and morphology of the nanodispersion were investigated. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis revealed that stigmasterol was loaded in nanodispersions successfully. The pectin, which was used to stable nanodispersion, could restrict the release of stigmasterol in the simulated gastric fluid. This experiment indicated that the presence of pectin can improve the stability of the nanodispersion and can be used to achieve controlled release of bioactive compounds.

Phytobiotic role of essential oil-loaded microcapsules in improving the health parameters in Clostridium perfringens-infected broiler chickens

Nowadays, the development of phytobiotics, an antibiotic-replacing agent, has attracted a considerable attention as poultry feed additive. The present study aims to develop the microcapsules loaded with a blend of essential oils (thyme, peppermint, savoury, and black pepper), and evaluate its health-promoting activity among the Clostridium perfringens-challenged broiler chickens. Based on the results, the spherical microcapsules containing a blend of thyme, summer savoury, peppermint, and black pepper essential oils had the particle size of 158.6 µm and encapsulation efficiency of 94.2%. The major bioactive compounds were thymol, carvacrol, p-cymene, and γ-terpinene. The microencapsulation of essential oil effectively prevented the release of bioactive phenolic compounds in the upper parts of the GIT. Additionally, it increased the delivery of the phenolic compounds to the lower segments of the GIT in the broiler chickens. The administration of the essential oil-loaded microcapsules (0.5, 1, and 2 kg/ton) in the C. perfringens-infected broiler chickens enhanced growth parameters, total feed intake, FCR, body antioxidant status, ileum morphostructure, and intestinal microbial population. Further, this treatment resulted in regulating the antioxidant and inflammation genes in ileum tissue. Consequently, the essential oil-loaded microcapsules can be considered as a promising phytobiotic against C. perfringens infection in broiler chickens. HIGHLIGHTS The spherical microcapsules containing a blend of EO had the particle size of 158.6 µm and encapsulation efficiency of 94.2%. The microencapsulation of essential oil effectively prevented the release of bioactive phenolic compounds in the upper parts of the GIT. The EO-loaded microcapsules led to an improvement in growth parameters, body antioxidant status, ileum morphostructure, and intestinal microbial population. The microcapsules regulated the antioxidant and inflammation genes in ileum tissue.

Fate of nano-phytosomes containing bioactive compounds of Echinacea extract in an acidic food beverage

• Simple centroid mixture design was used to optimize nano-phytosomes. • Echinacea extract-phospholipid phytosome was developed for oral consumption. • The design-optimized EPLP was added to an acidic food beverage. • The key properties of EPLP were evaluated in the beverage. Chicoric acid (CA) and chlorogenic acid (CGA) existing in the Echinacea plant have antioxidant activity but possess low stability and bioavailability. This study aims to improve oral bioavailability by formulating Echinacea extract (EE) phytosome developed for oral consumption. Echinacea extract phospholipid phytosome (EPLP) was optimized by applying a simple centroid mixture design with particle size, encapsulation yield, and the release of bioactive compounds in the simulated digestion media as response factors. Optimized EPLP was then added to an acidic food beverage and evaluated for stability, antioxidant activity, sensory properties, and in vitro release. Complexation of EE with phospholipids increased the stability and antioxidant activity of its bioactive compounds in the acidic food beverage for up to 30 days of storage. Furthermore, formulation as EPLP resulted in mask the poor taste of EE. The results indicate the potential of nanophytosomes to improve oral bioavailability of EE by increase the stability of its bioactive compounds in the acidic food beverage.

Evidence that protein corona reduces the release of antimicrobial peptides from polymeric nanocapsules in milk

The antimicrobial peptide produced by Bacillus velesensis P34 has a broad activity against Gram-positive bacteria, showing potential as natural food preservative. In this work, nanocapsules (NCs) containing the peptide P34 were produced using the polymers poly-ε-caprolactone (PCL) or Eudragit RS-100 (EUD), and their antimicrobial activities were assessed evaluating L. monocytogenes growth in synthetic media, milk and isolated milk proteins. As results, cationic and anionic nanocapsules were obtained, with zeta potential ranging from +15 to +28 mV for EUD and around −19 mV for PCL, and average diameter in the range of 104–130 nm and 224–245 nm, respectively. In the antimicrobial tests, only the P34-EUD NCs presented activity against L. monocytogenes in BHI broth, possibly due to the EUD high swelling and permeability properties, as compared with PCL. In whole and skimmed milk, the P34-EUD NCs caused no inhibition of L. monocytogenes growth, due to a possible interaction of casein proteins with the NCs surface resulting in protein corona formation, which interfered with the antimicrobial peptide release. Therefore, the application of polymeric NCs as antimicrobial delivery systems in foods could be limited by the polymer type, and the adhesion of specific matrix proteins that could form protein corona, reducing the bioactive compound release.

Effects of acid activation on the halloysite nanotubes for curcumin incorporation and release

Halloysite nanotubes (HNT) are natural aluminum silicate clays with a hollow tubular structure and chemical formula of Al2Si2O5(OH)•2H2O. The selectivity of its surface allows different types of treatments in order to improve some of its properties, such as pore volume and surface area, as well enabling the incorporation and release of drugs and bioactive compounds, such as curcumin, in a more efficient way. In this work, the HNT have undergone an acid treatment to incorporate curcumin. From the analysis of FTIR, TEM, XRD, SEM-EDS and nitrogen adsorption / desorption it was possible to suggest that activation with HCl on the nanotubes made it possible to obtain an increase in the specific surface area, in the volume of pores and in the lumen diameter, preserving the tubular structure of these materials. This process favored the incorporation of curcumin (Cur), which had a higher incorporation efficiency value of 77.42 ± 1.92% and an antioxidant activity of 75.91 ± 0.94%, and consequently 80% release after 90 min. With this study of in vitro release, TEM and TGA, it was possible to reveal that the incorporation of curcumin occurred in the lumen (diameter ≈ 23 nm) through electrostatic interactions between Al3+ and Cur−. In addition, the curcumin was partially adsorbed on the HNT pores surface, where a shift in Tonset of 15 °C was found for a lower temperature and an increase of 12 nm in the external diameter. These results indicate that acid activation in HNT favored the incorporation and release of Cur, which allows us to suggest the possibility of investigating the use of HNTA-C in the biomedical area, as in the treatment with antioxidant compounds.

Development and characterization of chitosan based polymer composite nanofibre as a smart carrier system for microbial immobilization

Electrospinning is a versatile and efficient technique which uses high electric field to produce ultrafine fibres from polymers. Nanofibers are widely used in biomedical and tissue engineering as scaffolds, sensors, filters, membranes, batteries, protective clothing, Wound dressing and as a catalyst. In agriculture, it is applied for controlled and targeted release of bioactive compounds. The main aim of this study was to develop E-spun nanofibre using biopolymer that act as a smart carrier system for beneficial bacteria to use it in seed coating. Polymer composite nanofibre was developed using chitosan and polyvinyl alcohol (PVA) by experimenting different ratio of PVA/Chitosan. The e-spin solution containing PVA and chitosan in the ratio of 8:2 was found to be optimum in producing fibre with desired diameter. The developed fibre was further characterized By Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Fourier Transform Infra-Red (FTIR) spectroscopy. The electron micrograph images provide the morphological structure of the PVA/Chitosan polymer composite nanofibre (100-150nm). Fourier Transform Infrared Spectroscopic (FTIR) measurement exhibited the interaction and existence of relevant functional groups of both PVA and chitosan in the nanocomposite fibres. This study provides a simple approach for the development of non-wovens that acts as a smart carrier to immobilize microbes which can be used for seed coating.

Nanostructured lipid carriers: Promising delivery systems for encapsulation of food ingredients

Abstract In the last few decades, various bioactive compound-delivery technologies have been emerged and nanoscale delivery devices have been developed. Nanostructured lipid carriers (NLCs) are delivery systems that reveal some advantages over conventional carriers such as increased loading capacity, encapsulation efficiency, solubility, storage stability, bioavailability, and half-life. Moreover, they provide controlled compound release and safety for food systems. In this review, aspects of NLCs, their components, production methods, bioactive compound incorporation, release, stability, and food applications are introduced.

Solid-state fermentation – assisted extraction of bioactive compounds from hass avocado seeds

The effect of solid-state fermentation (SSF) of avocado seed with A. niger GH1 on the release of bioactive compounds with antioxidant capacity was studied. The growing conditions of the fungus were optimized. Subsequently, The effect of sterile water and pontecorvo culture medium was evaluated in the SSF. The optimal conditions for SSF were particle size of 2.5 mm, 60 % of humidity, and 120 h. In the assessments of antioxidant capacity by the ABTS and DPPH methods, the increase with respect to the initial values was 33 and 77 % with water and 28 and 42 % with culture medium, respectively. The analysis HPLC showed degradation of catechin and epicatechin polymers, particularly procyanidin B2 dimers resulting in the accumulation of monomers. The ability of the fungus to degrade compounds present in the avocado seed was decisive to enhance the antioxidant capacity. This degradation was related to increased cellulase and xylanase activity.

A discovery-based metabolomic approach using UHPLC Q-TOF MS/MS unveils a plethora of prospective antihypertensive compounds in Korean fermented soybeans

Treating soybean with proteases and subsequent fermentation increases the release of bioactive compounds that may be strongly attached to the food matrix. In our quest to choose the best material to develop an antihypertensive functional food from soybean, we assessed the ability of raw soybean, Prozyme 2000p-hydrolyzed soybean (PSB) and Enterococcus faecium EBD1-fermented PSB to inhibit angiotensin converting enzyme (ACE). Treating soybean with Prozyme 2000p increased the ACE inhibitory ability from 15 ± 3% to 40 ± 2%. Subsequent fermentation of the hydrolyzed samples further increased the inhibitory ability to 80 ± 5%. A discovery-based metabolomic approach using UHPLC Q-TOF MS/MS of the fermented sample showed that E. faecium fermentation increased the levels of phenolic compounds, gamma aminobutyric acid, antioxidant and antihypertensive peptides. The fermentation also increased the levels of essential amino acids relative to the enzyme treated and raw samples. The high levels of potential antihypertensive compounds in the fermented samples imply that fermenting Prozyme-treated soybean with Enterococcus faecium EBD1 would be a good strategy for developing functional foods with antihypertensive properties.

Protein hydrolysates and phenolic compounds from fermented black beans inhibit markers related to obesity and type‐2 diabetes

AbstractObesity and type 2 diabetes mellitus (T2DM) represent an epidemic and public health problem. Bioactive compounds from foods could be used as adjuvants in the prevention of these diseases. Processes such as solid‐state fermentation (SSF) have demonstrated to improve the release of bioactive compounds from food matrix. This project aimed to evaluate the effect of fermented black bean (Phaseolus vulgaris L.) phenolic compounds and protein hydrolysates on markers associated with obesity and T2DM. Twenty‐eight peptide sequences and 12 phenolic compounds were identified on black bean samples. From in silico assays, sequenced peptides showed theoretical binding energies up to −9.8 kcal/mol and phenolic compounds up to −10.1 kcal/mol for monoacylglycerol lipase. Protein hydrolysates from SSF 96 h (12 peptides) showed the potential to inhibit α‐amylase with inhibitory concentration 50 (IC50) 0.57 mg protein/ml and 5.55 mg protein/ml for α‐glucosidase. Phenolic compounds from SSF 48 h blocked α‐glucosidase IC50 0.353 mg GAE/ml. The antioxidant capacity was maintained in phenolic compounds after fermentation for 48 h and improved in the protein hydrolysates. Raw bean protein hydrolysates (0.1 mg protein/ml) presented the potential to inhibit lipid accumulation in 3T3‐L1 cell line (27.9%). SSF is a processing method for generating functional ingredients rich in bioactive compounds capable of acting on biological markers related to the treatment or prevention of obesity and T2DM.

Ionogenic gel-forming (co)polymers of N-vinyl succinimide, N-vinylsuccinamic and acrylic acids grafted on starch

Gel-forming (co)polymers of N-vinylsuccinimide, N-vinylsuccinamic and acrylic acids grafted on starch were obtained using an initiating redox system or as a result of 60Co gamma irradiation. The dependence of the level of sorption properties (co)of polymers relative to bioactive compounds (BAC), their water absorption and swelling on the conditions of the (co) polymerization process has been determined. The ability of composite copolymers to high moisture absorption, binding and prolonged release of BAC and the possibility of their use for biomedical purposes has been established.

Modulating properties of polysaccharides nanocomplexes from enzymatic hydrolysis of chitosan

Synthesis of nanocomplexes is a simple and low-cost technique for the production of encapsulation systems aiming industrial applications, based on the interaction of at least two oppositely charged molecules. Gellan gum (anionic) is a water-soluble biopolymer resistant to stomach pH conditions, therefore an interesting alternative as an encapsulating matrix. Chitosan (cationic) is also widely used due to its biocompatibility and mucoadhesive properties, although its low water solubility is an important step to be overcome for the production of the complexes. To improve this property, many techniques have been employed, but most of them use unsustainable techniques and chemical agents. The enzymatic hydrolysis of chitosan using proteases emerges as an alternative to these drawbacks and, therefore, this study aimed to evaluate the electrostatic nanocomplexation of native (C) or hydrolyzed (HC) chitosan (by porcine pepsin protease) with gellan gum (G). Polysaccharides and nanocomplexes formed with different G:C or G:HC ratio were evaluated by zeta potential measurements, particle size distribution, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Transmission Electron Microscopy (STEM), intrinsic viscosity and turbidity analyses. Chitosan hydrolysis allowed the formation of a smaller (445.3 nm in pH 4.5) and more soluble structure (3 kDa), which positively influenced the formation of the complexes. The ratios G:HC of 7:3 and 8:2 formed complexes with lower values of zeta potential (13.9 mV and −5.0 mV, respectively), particle size (635.8 nm and 533.6 nm, respectively) and polydispersity (0.28 and 0.23) compared to complexes formed with native chitosan. Overall, our results show that enzymatic hydrolysis of chitosan favored the formation of electrostatic complexes with reduced size and low polydispersity, which can be used as efficient encapsulating matrices for improved targeted delivery and controlled release of bioactive compounds.

In Vitro Evaluation of a Fluorescent Microemulsion as an Oral Delivery Carrier and its Potential Application in Tracking Bioactive Compounds Label-Free.

In this work, a microemulsion emitting fluorescence was fabricated as a potential oral delivery system for bioactive compounds. In simulated oral administration, the microemulsion was characterized for its microstructure by 1hydrogen-nuclear magnetic resonance (1H-NMR). Results showed that microemulsions not only have good resistance to oral and gastric phases, but also lay a solid foundation for the release of bioactive compounds in the intestine. Fluorescence stability tests showed that microemulsions exhibit a remarkable fluorescence intensity in the digestive environment, indicating feasibility as a label-free delivery carrier. Moreover, in vitro release tests of bioactive compounds confirmed that an α-linolenic acid (ALA)-loaded microemulsion mainly released in the intestine, thereby achieving the aim of controlling the release of bioactive compounds. These results suggest that the synthesized fluorescent microemulsion, combining the favorable features of nontoxicity, antidigestive stability, remarkable fluorescence intensity, and controllable release, can be regarded as a promising label-free delivery carrier for oral administration.

Encapsulation of camu-camu extracts using prebiotic biopolymers: Controlled release of bioactive compounds and effect on their physicochemical and thermal properties

Camu-camu (Myrciaria dubia [H.B.K] McVaugh) is a Amazonian fruit rich in ascorbic acid and phenolic compounds, and has been attracting great interest from the food, pharmaceutical and nutraceutical industries due to its potential health benefits. The bioactive compounds from camu-camu are considered sensitive and unstable, resulting in nutritional losses and impairment of its commercialization and export. For this reason, the camu-camu extract (pulp and peel) was subjected to microencapsulation by spray drying process using maltodextrin (MD), inulin (IN), and oligofructose (OL) as carrier agents. Lyophilized in natura camu-camu extract (CEL) was also evaluated. Thus, physicochemical and thermal properties and controlled release at different temperatures (25 °C and 35 °C) were investigated. In contrast with the IN and OL microparticles, the MD microparticles showed lower density and hygroscopicity, besides greater thermal stability, antioxidant activity, and retention of ascorbic acid and anthocyanins. FTIR spectra allowed the qualitative evidence of encapsulation of the bioactive compounds from the camu-camu extract. The highest percentage of volatile compounds was observed in IN microparticles, followed by OL and MD microparticles. The major group of compounds identified in CEL were terpenes (88%). The Korsmeyer-Peppas mathematical model allowed to describe the controlled release behavior of ascorbic acid and anthocyanins in the powder extracts. The controlled release followed a Fickian diffusion mechanism (n ≤ 0.43). The increase of temperature from 25 °C to 35 °C influenced on the release of bioactive compounds in all treatments, showing greater release for MD microparticles. The encapsulating materials were considered effective for the production of camu-camu extract powder, contributing to the better use of this Amazonian fruit. In addition, the encapsulation process increased the stability of its bioactive compounds, representing a tool to facilitate their incorporation in several matrices to act as antioxidant and coloring agents, as well as nutraceutical.