Bioaccumulation Research Articles

Biosorption Property of Cadmium (II) in <i>Marsilea minuta </i>L. with Polyamine Interaction

A study was undertaken for biosorption following bio accumulation property of Marsilea minuta L., an aquatic pteridophyte in cadmium enriched solutions. The possible mechanism for biosorption on the cell wall residues has been discussed with exogenous applications of polyamine. Initially plants were evaluated both with fresh and dry mass for their differential adsorption. A typical distribution of metal in different tissues, more into cortical and endodermal apoplastic regions were the features as detected by Energy Dispersive Analysis of X-ray study. The changes of the cell wall residues and their possible interaction with metals have been analyzed with Fourier Transform Infrared Spectrometric studies. With this study the most possible contributing chemical bond was detected as alkyl halide with its possible changes in energy absorption. This was also minimized by spermidine application and thus established its involvement for biosorption of metal. Accumulation of Cd in relation to chemical mechanism has been discussed in Marsilea and analyzed its efficacy as a well hyper-accumulator under metal contaminated environment.

Nuclear and spectrochemical techniques in developmental metal toxicology research. Whole-body elemental composition of Xenopus laevis larvae

Instrumental and radiochemical neutron activation analysis (INAA and RNAA, respectively) in combination with inductively coupled plasma mass spectrometry were used for the measurement of 52 constituent elements in 120 h Xenopus laevis larvae. With this approach macro and trace elements were measured in X. laevis larvae samples. The validation of the analytical techniques was performed by using certified reference materials and by recovery tests. Our feasibility study appears relevant for future studies on the bioaccumulation patterns of elements in Xenopus as effective indicators of environmental metal contamination.

A novel method applied in determination and assessment of trace amount of lead and cadmium in rice from four provinces, China.

Heavy metal contamination of soils or water can lead to excessive lead (Pb) and cadmium (Cd) levels in rice. As cumulative poisons, consumption of Pb and Cd in contaminated rice may cause many toxic effects in humans. In the present study, Pb and Cd levels in rice samples from Hubei, Jiangxi, Heilongjiang, and Guangdong provinces in China were analyzed by cloud point extraction and graphite furnace atomic absorption spectrometry (GFAAS). The heavy metals in the rice samples were reacted with 8-quinolinol to form a complex at pH 9.0 and 40°C. Analytes were quantitatively extracted to a surfactant-rich phase (Triton X-45) after centrifugation and analyzed by GFAAS. The effects of experimental conditions, including pH, concentration of reagents, and equilibration time and temperature, on cloud point extraction were optimized efficiently using Plackett–Burman and Box–Behnken experimental designs. Under the optimum conditions, good linearity was observed in the concentration ranges of 0.5–5 µg/L for Pb and 0.05–0.50 µg/L for Cd. The limits of detection were 0.043 µg/L for Pb with a concentration factor of 24.2 in a 10 mL sample and 0.018 µg/L for Cd with a concentration factor of 18.4 in a 10 mL sample. Twenty rice samples from four provinces were analyzed successfully, and the mean levels of Pb and Cd in the rice were all below their maximum allowable concentrations in China. Comparing the tolerable daily intakes given by FAO/WHO with the mean estimated daily intakes; Pb and Cd mean daily intake through rice consumption were 0.84 µg/kg bw/day and 0.40 µg/kg bw/day, which were lower than the tolerable daily intakes.

TOPRAK VE AKASYA AĞACI SÜRGÜNLERİNDEKİ İZ / AĞIR METAL DAĞILIMI, GÜMÜŞHANE - TÜRKİYE

In this study, heavy metal/trace element accumulation was investigated in soils along theroad passing through Gumushane city center and shoots of 1-2 years of acacia trees(Robiniapseudoacacia L.) grown in these soils. Heavy metal contents in soils and plantswere analyzed by Enrichment Factor (EF), Geo-accumulation index (Igeo) parameters,and by Bio Accumulation Factor (BAF), respectively. According to geo-accumulationindex (Igeo) data, it is seen that the soil was unpolluted in terms of Cr, Co, Cu, Rb, Sr;unpolluted to moderately polluted in terms of V, Ni and Zn; moderate to excessivelypolluted in terms of As, and moderate to excessively polluted in terms of Pb. According toEF parameters, on the other hand, it is observed that the soil was non to slightly enrichedin terms of Cr, Co, Sr and Ba; non to moderately enriched in terms of Ni and Cu; slightlyto significantly enriched in terms of Zn; significant to very highly enriched in terms of Asand slightly to over excessively enriched in terms of Pb. Although trace/heavy metalcontents of acacia shoots were usually within normal values for acacia, it was determinedthat Cu, Fe, Mo, Ni, Sr and Zn concentrations were within and/or above upper limits ofnormal values in certain sampling points.1. Girifl

The Toxicity Effect of Vanadium on Artemia franciscanna and Artemia urmiana

The presened research was carried out in order to study how the toxicity of such heavy metals as vanadium affects on the remains of nauplius and the growth and life cycle of Artemia urmiana and Artemia franciscanna. The in 24 h of A. urmiana and A. franciscana exposed to vanadium were 0.0107 ,0.011 mg/l respectively. In growth experiments, the length of animals was considered as growth index. Results indicates that the mean length of animals in (0.001, 0.002 and 0.003 mg/l) V on first, 5th, 7th and 11th days of life significantly decreases in comparison with control groups (p<0.05).Bioaccumulation of V in the same concentration, after 24 h in nauplius and also in adults of A. urmiana and A. fransicana were statistically significantly higher than of the control groups (P < 0.05). Both species accumulate vanadium in their bodies. However A. urmiana is more resistant to the heavy metals. Results show, vanadium is high toxic on Artemia.

A field study of the relationship between sulfide-bound metals and bioaccumulation by Limnodrilus sp. in a heavily polluted river

Acid volatile sulfide (AVS) has been regarded as an important factor controlling metal bioavailability in anoxic sediments, but its effect on metal accumulation under natural conditions is poorly understood. Here, a field study of the influence of AVS on metal accumulation by Limnodrilus sp. in a heavily polluted river is provided. Most of the study area was subject to anaerobic and strongly reducing conditions, and the concentration of trace metals in surface sediments was high, as were the concentration of AVS and simultaneously extracted metals (SEM; average AVS = 20.3μmolg(-1), average ∑SEM5 = 9.42μmolg(-1); ∑SEM5 refers to the sum of SEMCd, SEMCu, SEMPb, SEMNi, and SEMZn). Only a few species and small quantities of benthic invertebrates were found, and Limnodrilus sp. was dominant. There was no correlation between trace metal accumulation and (SEM-AVS), and in stations where (SEM-AVS) <0, the absolute value of bioaccumulation was high (average ∑BIO5 = 4.07μmolg(-1); ∑BIO5 refers to the sum of BIOCd, BIOCu, BIOPb, BIONi, and BIOZn), indicating that there was no relationship between (SEM-AVS) and metal accumulation in Limnodrilus sp. This was likely because Limnodrilus sp. ingest sediment particles as their main food source, so pore water metals play a minor role in their bioaccumulation (BIO) of materials. However, ∑BIO5 was significantly correlated with ∑SEM5 (r = 0.795, p < 0.01), revealing that the large number of sulfide-bound metals (SEM) in sediments may play an important role in metal accumulation in Limnodrilus sp., which can assimilate sulfide-associated metals by the help of the digestive fluids in the digestive systems.

AYURVEDIC ASPECT OF LEAD AND ITS CHRONIC TOXICITY

Chronic Lead toxicity is found in form of occupational or envi ronmental exposure with leading encounter during manufacturing, transportation and utilisation of Lead and its compounds. Though information about the Lead toxicity is not found in Brihatrayi like Charak , Shushrut , but it is found in text book of Ras Taran gini, Ayurved Prakash, Vrahat Ras Raj Sundar etc. in detail. As the Lead toxicant accumulate on cellular level after chronic and persistent exposure like Cumulative Poison ( Dushee Visha ) and its toxic effect after existence of some aggregative factors. It vitiates all the dosha, but Vata and Pita is vitiated more pre dominantly. It affects all the system (strotas), but Central nervous s ystem (Majava strotas), Haematology (Ras - Raktavaha Strotas) and Gastro intestinal tract (Annavaha strotas and Purishvaha Str otas) are more affected. There is no such treatment of chronic lead toxicity found in Ayurvedic Or iginal Text, but Enema (Basti) t herapy after Bio - purification and then symptomatic management may give

Caffeine (1,3,7-Trimethylxanthine) as a modulator of Arsenic bioaccumulation in the experimental Pelecypod model, Lamellidens corrianus

Aims: The present investigation was carried out to study modulator effect of caffeine (1,3,7-Trimethylxanthine) on Arsenic-induced alterations on freshwater bivalve, Lamellidens corrianus. Materials and Methods: Freshwater Pelecypod Mollusc, L. corrianus were exposed to acute dose of arsenic (0.672 ppm As 3+ ) for 4 days. Arsenic exposed Bivalves were allowed to cure naturally and with caffeine. Testis and digestive glands from different groups were removed and dried in the oven. The dry powders were digested in nitric acid and perchloric acid in 4:1 ratio at hot temperature till dryness. The digest was dissolved in double glass distilled water and the arsenic contents were estimated by the Atomic Absorption Spectrophotometer. Results: After 4 days of exposure, the amount of bioaccumulated arsenic in the testis and digestive glands was 1.044 and 1.119 μg/g. During the recovery after 4 days, the bioaccumulated arsenic was reduced to 0.969 and 1.067 μg/g of normal water while in caffeine exposed Bivalves was reduced to 0.917 and 0.975 μg/g in testis and digestive glands respectively. Conclusion: Rapid reduction in the arsenic contents in caffeine exposed Bivalves indicates the role of caffeine in arsenic excretion. Therefore, the caffeine (1,3,7-Trimethylxanthine) as a modulator of Arsenic bioaccumulation in the experimental Pelecypod model, L. corrianus

Content of zinc in plants depending on its fractional composition in soil and properties of soil

Metals in soils occur in different forms, which are more or less mobile, which means bioavailable. The aim of this study was to assess the content of zinc in cultivated plants, depending on soil properties but also its content in soil and fractions in typical and unpolluted region, which is the Podlasie Province. The research material consisted of samples taken from arable soil in 81 points of Podlasie Province. Basic physicochemical properties and content of soil fraction < 0.02 mm in samples were determined. Pseudo-total content of zinc and its fractional composition in soil as well as zinc content in plants was evaluated. Correlation coefficients between the content of zinc in plants and physicochemical properties of soil were calculated. It was found that examined soil contained natural amount of zinc, characteristic for uncontaminated soils. The most of zinc was associated with the organic matter fraction and the least with residual fraction. Among the analysed plants from light soils, the most of zinc was found in rye. Plants from medium-heavy soils contained similar amount of zinc, with the exception of maize. Correlation coefficients do not confirm the significant relationship between soil properties and zinc in plants. Significant relationship was stated between bioaccumulation factors and the content of zinc in plants.

Macrophytes as indicators of heavy metals bio-accumulation in upper Narew River

Macrophytes as indicators of heavy metals bio-accumulation in upper Narew River

White muscle as a bio-indicator of cadmium (Cd) pollution across Kalpani River Mardan, Khyber Pakhtunkhwa Pakistan

In the present investigation we examined the bio accumulation of 05 heavy metals (Pb, Cr, Cd, Ni, Zn) in the muscle tissue of fresh water fish Mulley, Wallago attu collected from 04 different polluted sites of Kalpani River in Khyber Pakhtunkhwa province Pakistan, during July 2012 to February 2013. The heavy metals concentration in the muscle of Wallago attu was determined by using Perkin Elmer AS 3100 flame atomic absorption spectrophotometer. Heavy metals accumulated in the order Cd>Cr>Zn>Ni in the muscles tissue of Wallago attu, with no detection of lead (Pb) at all. Cadmium (Cd) was the highest and nickel (Ni) was the least accumulated heavy metal in fish white muscle. Mean concentrations of detected four heavy (Cr, Cd, Ni, Zn) in the muscle of Wallago attu in all 04 samples were 0.44±0.4201, 0.84±0.8283, 0.15±0.1056, 0.31±0.357 μg g -1 (wet weight). Overall, cadmium (Cd) was more accumulated heavy metal in fish muscle as compared to other heavy metals. Our findings suggest that fish muscle have comparatively more chemical affinity to bio accumulate cadmium (Cd) as compared to heavy metals like Zn, Cr and Ni. That is why, cadmium (Cd) is suggested to be a good bio indicator for cadmium (Cd) pollution in aquatic environment.

The study of zinc bioaccumulation in internal organs of swan mussel, Anodonta cygnea (Linea, 1876)

Heavy metals as importantpollutants in aquatic ecosystems can cause adverse biological effects on liveorganisms of these environments. This research was conducted to study the bioaccumulationof zinc (Zn) in internal organs (mantle, gill, hepatopancreas and foot) of thefreshwater bivalve, Anodonta cygnea during 27 days in vitro exposureperiod. Results showed that this species has a high potency of metalaccumulation. There was no significant difference between basal Zn levels instudied organs. Maximum accumulation rate were recorded in gill andhepatopancreas and their Zn content was significantly (α=0.05; P < 0.02) higher than mantle and foot. The minimumZn accumulation occurred in mantle. Zn levels in gill, hepatopancreas and footwere increased during exposure period, but a decreasing trend was observed inZn level of mantle between days 18 and 27. The results of this study indicatedthat accumulation potential of different organs and their sensitivity andimpressibility are different and gill and hepatopancreas are appropriate organsto study heavy metal bioaccumulation and their pollution in freshwaters.

Bioaccumulation profile of heavy metals in the gills tissue of Wallago attu (MULLEY) from Kalpani River Mardan, Khyber Pakhtunkhwa Pakistan

We examined the bio accumulation profile of 05 heavy metals (Pb, Cr, Cd, Ni and Zn) in the liver tissue of fresh water fish Mulley, Wallago attu collected from 04 different polluted sites of Kalpani River in Khyber Pakhtunkhwa province Pakistan. The heavy metals concentration recorded in the liver of Wallago attu was determined by using Perkin Elmer AS 3100 flame atomic absorption spectrophotometer. Metals bio accumulated in the liver tissue in the order of Cd>Cr>Zn>Ni with no detection of lead (Pb) in any fish sample. Cadmium (Cd) was the highest and nickel (Ni) was the least accumulated metal in the fish liver. Mean concentrations of detected four heavy (Cr, Cd, Ni, Zn) in the liver of Wallago attu in all 04 samples were 0.90±0.8118, 3.33±1.3595, 0.04±0.0250, 0.72±0.1937 μg g -1 (wet weight). Overall, cadmium (Cd) was more accumulated in the fish liver as compared to other heavy metals. At present cadmium has crossed the permissible limits of FAO (1983). Our findings suggest that fish liver have comparatively more chemical affinity to bio accumulate cadmium (Cd) as compared to heavy metals like Zn, Cr and Ni.

Human bioaccumulation potential simulated in R and implemented into KNIME interface

Human bioaccumulation potential simulated in R and implemented into KNIME interface

Evaluation of polybrominated diphenyl ethers in sediment of Lagos Lagoon, Nigeria

Certain pollutants, particularly synthetic organic compounds have given rise to important environmental concerns. New organic pollutants especially polybrominated diphenyl ether (PBDEs) employed in electronic equipment and in some household items as flame retardants are now finding their way into the aquatic environment as components of waste discharge into the water body. These highly hazardous organic pollutants of concern are persistent, can bioaccumulate and biomagnify in aquatic organism especially fish, and there appears to be no clear strategy for managing them. In this study, levels of polybrominated diphenyl ethers were determined in sediments collected from Lagos lagoon with the aim of generating a database which can be employed for management options. Sediment samples were collected using Van Veen grab for a period of one year from randomly selected sites in Lagos lagoon. The samples were soxhlet extracted with dichloromethane to obtain PBDEs extracts which were later cleaned up in a column of silica gel using hexane as eluant. The cleaned extracts were analyzed using gas chromatography coupled with electron capture detector. The total concentrations of PBDEs in sediments ranged from 0.11 to 23.33 mg/kg. In all the studied locations, BDE-28, BDE-153, BDE-154 and BDE 205 were detected in all sediment samples at concentration range of 0.22 to 23.33 mg/kg. Among the PBDEs congeners, sum of tri to hepta BDEs contributed 61.32%, while BDE-205 contributed 38.68% to the total PBDE in the sediment samples. Brominated congeners BDE-47, BDE-153 and BDE-154 (tetra and hepta BDEs) were abundant which contributed 18.31, 12.06 and 34.75%, respectively to the sum of tri to hepta BDE in the sediment. The composition patterns of PBDEs in Lagos Lagoon sediment samples revealed that technical deca-BDE mixture was the major pollutant sources with a minor contribution of penta-BDE mixture. Key words: Gas chromatograph, polybrominated diphenyl ether (PBDEs), sediment, Lagos Lagoon

Bioaccumulation of heavy metal uptake by two different Vetiver grass (Vetiveria zizanioides and Vetiveria nemoralis) species

The efficiency of Cd, Pb and Zn uptake of upland vetiver grass (Vetiveria nemoralis) ecotypes as Kamphaeng Phet1, Prajuab Kirikhun, and Ratchaburi and lowland vetiver grass (Vetiveria zizanioides) ecotypes consisting of Kamphaeng Phet2, Mae Hongson, and Surat Thani were compared under sterile condition for interferent repudiation. The results showed that the total Cd accumulation of V. zizanioides was in the order of Kamphaeng Phet1 > Ratchaburi > Prajuab Kirikhun ecotype and in V. nemoralis was Mea Hongson approximately Kamphaeng Phet2 > Surat Thani (p < 0.05). All the vetiver grass ecotypes preferentially accumulated Pb in roots than shoots, with the rate of translocation less than 1. However, the efficiency of Cd and Zn translocation was different, depending on each vetiver ecotype. In addition, all vetiver ecotypes, except Kamphaeng Phet1, displayed high capability of Zn uptake in both shoots and roots after 7 days. Therefore, the vetiver plant can be considered a good “hyperaccumulator” only for Zn. Key words: Vetiveria zizanioides, Vetiveria nemoralis, total accumulation rate (TAR), transport factor (TF).

Heavy metal concentrations in water, sediment and periwinkle (Tympanotonus fuscastus) samples harvested from the Niger Delta region of Nigeria

Heavy metal pollution of terrestrial and aquatic environments in Niger-delta region of Nigeria is on the increase due to increased urbanization and crude oil exploration. Tympanotonus fuscastus are mud dwellers and could bioaccumulate heavy metals; therefore consumption of heavy metal contaminated sea foods like periwinkle may breed lots of health problems. Heavy metal concentration in water, sediment and Periwinkle samples from three locations (Itu-River, Abuloma River and Oron River) in the Niger Delta Region of Nigeria were evaluated using atomic absorption flame photometry. Result showed that cadmium (Cd) concentration was highest in water samples from Abuloma River (0.106 mg/l) while lead (Pb) concentration was highest (0.01mg/l) in the water samples from Itu River. Cd and Pb concentrations (0.127 and 0.08 mg/kg, respectively) in sediment samples were highest in Abuloma River. The concentration of Cd in T. fuscastus samples was 0.11 mg/kg in Abuloma River, while concentration was 0.27 mg/kg in Oron River. Copper (Cu) was generally low in the water samples; the highest concentration (0.011 mg/kg) was obtained in water samples from Oron River. Sediment concentration of Cu was high (0.088 mg/kg) in Itu River, while its concentration in the periwinkle samples was 0.54 mg/kg in Abuloma River. The results also showed that Cr, As and Hg were below detectable concentration in tissues, soil and water samples from Itu and Abuloma Rivers, while Hg concentration in Oron River sediment was 64.2 mg/kg. Key words: Sea foods, heavy metals, pollution, environment, nutrition, bioaccumulation

Estimation of effects of tributyltin (TBT) bioaccumulation using the imposex character in marine gastropods (Thais tuberosa and Monodonta labio)

The effects of tributyltin (TBT) have been estimated using imposex characters in two species of marine gastropods, Thais tuberosa and Monodonta labio, at 3 different locations along the Minahasa Peninsula, northern part of Sulawesi Island: Manado Bay Waters, Bitung Coastal Waters, and Tateli Coastal Waters as a control area. The estimation was done by comparing the percentage of female imposex (I) at different body size (small: shell length ≤ 25 mm; large: shell length &gt;25 mm), and measuring the concentration of TBT in sediments and soft part of the gastropod samples. The highest I value was found in small sized T. tuberosa from Manado Bay Waters (63%) and large sized T. tuberosa from Bitung Coastal Waters (60%). Small and large sized M. labio had their highest I value, 30% and 44% respectively, in the samples from Manado Bay Water and Bitung Coastal Waters. The occurence of imposex in both speciesis correlated with the high concentration of TBT in sediments and soft part of the marine gastropod samples© Kajian tentang pendugaan pengaruh bioakumulasi logam berat tributyltin (TBT) menggunakan karakter imposeks pada gastropoda laut (Thais tuberosa dan Monodonta labio) telah dilakukan. Sampel diambil dari 3 lokasi di perairan sepanjang Semenanjung Minahasa, Pulau Sulawesi bagian Utara, yaitu Perairan Teluk Manado (PTM), Perairan Bitung (PBT), dan Perairan Pantai Tateli (PPT) sebagai lokasi kontrol. Pendugaan dilakukan dengan cara membandingkan persentase individu imposeks (I) sampel ukuran kecil (K; panjang cangkang ≤ 25 mm) dan ukuran besar (B; panjang cangkang &gt;25 mm), dan pengukuran konsentrasi TBT pada sedimen dan bagian lunak sampel gastropoda laut. Nilai I tertinggi adalah pada T. tuberosa ukuran K asal PTM, sebesar 63%, dan ukuran B asal PBT, sebesar 60%.Untuk M. labio baik ukuran K maupun B, nilai I tertinggi adalah pada sampel asal PTM dan PBT, masing-masing sebesar 30% dan 44%. Kejadian imposeks pada kedua jenis sampel ini didukung dengan konsentrasi TBT pada sedimen dan jaringan gastropoda laut yang tinggi©

Soil-water interaction in soils of the Po River Plain (Ferrara, Northern Italy): insights on heavy-metal mobility and phytoavailability

The soils of the Po River plain, developed on the alluvial sediments, are often characterized by high concentration of heavy metals, in particular chromium and nickel. These geochemical anomalies are “geogenic”, i.e. related to the specific nature of the “mother rocks” outcropping in the basin that typically include mafic and ultramafic lithologies. The elevated heavy metal backgrounds of soils potentially represent an effective geochemical risk considering the toxicity of these elements. In order to delineate soil quality thresholds and to provide guidelines for human activities (e.g. agriculture) the current legislation takes into consideration the “pseudototal” metal content obtained with aqua regia extraction tests. However, only a fraction of this chemical budget is available for plant and human uptake. Soil leaching tests with deionized water plausibly provide a better analogue to simulate the natural soil-water interactions, in order to predict the behavior of metals in the environment. In particular, in this paper we investigate with water leaching tests soils sampled in the surrounding of Ferrara (eastern part of the Po plain) that were previously characterized by bulk XRF analyses and aqua regia extractions. The approach gives insights on the element-specific transport parameters, giving clues for a) the possible contamination of the natural water and b) the soil-to-plant uptake processes and phytoavailability. Results, expressed as solid-water partition coefficients, highlight that nickel is significantly more mobile and bioaccessible than chromium and should be monitored in the local agricultural products to avoid its possible transfer and bioaccumulation in the food chain.

Performance experiments of rotary cage atomizer for biological pesticide application

To combat plant pests and diseases, spraying pesticide is an efficient and timely control method. The increasing use of a wide range of toxic chemical pesticides deliberately released into the environment can cause important problems such as human health threats, bioaccumulation and pest resistance, and soil and groundwater pollution. In response to concerns about the effects of chemical pesticides on environments and human health, there have been significant interests in biological pesticide technology with low toxicity, less residue, and reduced pest resistance. Biopesticide technology is limited in real applications, due to the lack of appropriate sprayer and standard technology. In this paper, a rotary cage atomizer for biopesticide was developed. The influence of biopesticide atomizer structure (cage diameter and cage mesh number), operational parameters (flow rate and rotational speed), and liquid types (bacillus thuringiensis and beauveria bassiana) on atomization performance and biopesticide application efficacy was investigated using PIV and a Winner laser diffraction particle analyzer. The pattern of liquid inlet was fixed experimentally by the droplet's flow field uniformity. The impacts of individual factors on droplet size were analyzed using a T-test to eliminate inter-correlation among the variables of interest. A multiple linear regression model, which predicted the volume median diameters (VMD), was established. The model showed a high degree of correlation with the experimental results. The model took into account not only the cage diameter, but its number of cage meshes, the liquid flow rate, and rotational speed of the cage. Furthermore, a histogram and normal P-P plot of standardized residuals were used to assess the model's goodness-of-fit and the measured data were also analyzed to prove model fitness and feasibility. Finally, the optimal combination of biopesticide rotary atomizer structure and operational parameters was implemented. The results indicated that the cage atomizer's liquid inlet of bilateral symmetry is better than the unilateral pattern to improve the distribution uniformity of droplets. The rotational speed of the rotary cage atomizer should not be too high to maintain the viability and infectivity of biopesticides. Results from this paper provide a case study showing how to spray biopesticide to treat plant pests and diseases effectively by using proper sciences and technologies.