Biotreatment Of Wastewater Research Articles

Wastewater biotreatment and bioaugmentation for remediation of contaminated sites at an oil recycling plant

ABSTRACT This work focused on the biotreatment of wastewater and contaminated soil in a used oil recycling plant located in Bizerte. A continuous stirred tank reactor (CSTR) and a trickling filter (TF) were used to treat stripped and collected wastewater, respectively. The CSTR was started up and stabilized for 90 days. Over the following 170 days, the operational organic loading rates of the TF and the CSTR were around 1,200 and 3,000 mg chemical oxygen demand (COD) L−1 day−1, respectively. The treatment efficiency was 94% for total petroleum hydrocarbons, 89.5% for COD, 83.34% for biological oxygen demand (BOD5), and 91.25% for phenol. Treated industrial wastewater from the TF was used for bioaugmentation (BA) of contaminated soil. The assessment of the soil took 24 weeks to complete. The effectiveness of the soil BA strategy was confirmed by monitoring phenolic compounds, aliphatic and polycyclic aromatic hydrocarbons, heavy metals, and germination index. The biodegradation rate of contaminants was improved and the time required for their removal was reduced. The soil bacterial communities were dominated by species of the genera Mycobacterium, Proteiniphilum, Nocardioides, Luteimicrobium, and Azospirillum, which were identified as hydrocarbon and phenol-degrading bacteria.

Enhanced anaerobic reduction of nitrobenzene under hypersaline fluctuation: Glycine betaine metabolism and microbial osmoadaptation mechanisms

High salinity, particularly large fluctuations in salinity, poses a significant threat to the biological treatment of high-salt industrial wastewater, while glycine betaine (GB) is commonly used for osmotic pressure balance. However, the GB metabolism mechanism and the effect of GB addition on microbial osmoadaptation responding to salt perturbations are still unclear. This study investigated the effect of GB on the stability of anaerobic process in nitrobenzene reduction under various salt perturbations and further explored the microbial osmoadaptation and metabolism mechanism. The nitrobenzene reduction was observably improved with GB addition (R+), and the microbial resilience increased due to repeated salt shocks. The generation of acetic acid showed a 46.21 % decrease in R+ compared to that without GB addition (R-) at 3 % salinity. Meanwhile, the intracellular GB concentration reached 10.76 mg L−1 to 54.76 mg L−1 with salinity increasing from 1.5 % to 6 %. 16S rRNA analysis revealed that the osmoadaptation period was shortened in R+ through the rapid osmoadaptation of salt-intolerant microorganisms (such as Propionibacteriaceae and SBR1031) and the massive enrichment of salt-tolerant microorganisms (such as Glutamicibacter). Besides, the resistance to hypersaline disturbance improved by the increasing size and complexity of the microbial community network. Moreover, the metagenomic analysis revealed that the abundance of genes involved in microbial metabolism was significantly enhanced in R+, indicating a more efficient metabolic efficiency and electrons transfer with GB addition under high-salt environments. This study provides vital insights into GB metabolism and microbial osmoadaptation responding to salt perturbations in long-term high-salt wastewater biotreatment.

Removing high strength lincomycin in pharmaceutical wastewater by a bacteria microalgae consortium co-immobilized filter

Lincomycin (LIN) in pharmaceutical wastewater would enter municipal wastewater treatment plants and decrease their performance, leading to residual LIN enter the natural environment and pose serious eco-risk. In this study, a bacterium-microalgae consortium co-immobilized filter (BMCCF) was established and used to remove LIN in artificial pharmaceutical wastewater treatment plants effluents (PWWTPE). LIN removal mechanisms and degradation products’ eco-toxicity was studied, and the abundance change of class 1 integrase gene (intⅠ1) and antibiotic resistance genes (ARGs) was monitored. As a result, 98.54 % of 82 mg/L LIN was removed within 7 days, LIN removal was mainly attributed to bio-degradation by the Bacillus subtilis strain, and LIN degradation products were less toxic than their substrate. Therefore, the BMCCF established in this study provides a promising alternative for the bio-treatment of pharmaceutical wastewater containing high concentration of LIN.

RETRACTED: Heredia-R et al. Evaluation of a Microbial Consortium and Selection of a Support in an Anaerobic Reactor Directed to the Bio-Treatment of Wastewater of the Textile Industry. Sustainability 2022, 14, 8889

The journal retracts the article “Evaluation of a Microbial Consortium and Selection of a Support in an Anaerobic Reactor directed to the Bio-treatment of Wastewater of the Textile Industry” [...]

Effects of Retention Time, pH, Temperature and Type of Fruit Wastes on the Bioelectricity Generation Performance of Microbial Fuel Cell during the Biotreatment of Pharmaceutical Wastewater: Experimental Study, Optimization and Modelling

Effects of Retention Time, pH, Temperature and Type of Fruit Wastes on the Bioelectricity Generation Performance of Microbial Fuel Cell during the Biotreatment of Pharmaceutical Wastewater: Experimental Study, Optimization and Modelling

Sequential bio-treatment of ammonia-rich wastewater from Chinese medicine residue utilization: Regulation of dissolved oxygen

To effectively treat actual ammonia-rich Chinese medicine residue (CMR) resource utilization wastewater, we optimized an anaerobic-microaerobic two-stage expanded granular sludge bed (EGSB) and moving bed sequencing batch reactor (MBSBR) combined process. By controlling dissolved oxygen (DO) levels, impressive removal efficiencies were achieved. Microaeration, contrasting with anaerobic conditions, bolstered dehydrogenase activity, enhanced electron transfer, and enriched the functional microorganism community. The increased relative abundance of Synergistetes and Proteobacteria facilitated hydrolytic acidification and fostered nitrogen and phosphorus removal. Furthermore, we examined the impact of DO concentration in MBSBR on pollutant removal and microbial metabolic activity, pinpointing 2.5 mg/L as the optimal DO concentration for superior removal performance and energy conservation.

Effective thiocyanate biodegradation mediated by bacteria associated to Argentinean mining areas potentially applicable to wastewater biotreatment

Effective thiocyanate biodegradation mediated by bacteria associated to Argentinean mining areas potentially applicable to wastewater biotreatment

Mechanisms linking triclocarban biotransformation to functional response and antimicrobial resistome evolution in wastewater treatment systems

Evaluating the role of antimicrobials biotransformation in the regulation of metabolic functions and antimicrobial resistance evolution in wastewater biotreatment systems is crucial to ensuring water security. However, the associated mechanisms remain poorly understood. Here, we investigate triclocarban (TCC, one of the typical antimicrobials) biotransformation mechanisms and the dynamic evolution of systemic function disturbance and antimicrobial resistance risk in a complex anaerobic hydrolytic acidification (HA)-anoxic (ANO)/oxic (O) process. We mined key functional genes involved in the TCC upstream (reductive dechlorination and amide bonds hydrolysis) and downstream (chloroanilines catabolism) biotransformation pathways by metagenomic sequencing. Acute and chronic stress of TCC inhibit the production of volatile fatty acids (VFAs), NH4+ assimilation, and nitrification. The biotransformation of TCC via a single pathway cannot effectively relieve the inhibition of metabolic functions (e.g., carbon and nitrogen transformation and cycling) and enrichment of antimicrobial resistance genes (ARGs). Importantly, the coexistence of TCC reductive dechlorination and hydrolysis pathways and subsequent ring-opening catabolism play a critical role for stabilization of systemic metabolic functions and partial control of antimicrobial resistance risk. This study provides new insights into the mechanisms linking TCC biotransformation to the dynamic evolution of systemic functions and risks, and highlights critical regulatory information for enhanced control of TCC risks in complex biotreatment systems.

Semi-Industrial Preparation of Biologically Active dyed fabrics Treated with Nano-metal oxides Impart them Multiple Functions for use in Potential Medical Approaches: Chemical and Bio-Treatment of Wastewater of Dyeing baths Effluents in an Environmentally Safe Manner

Semi-Industrial Preparation of Biologically Active dyed fabrics Treated with Nano-metal oxides Impart them Multiple Functions for use in Potential Medical Approaches: Chemical and Bio-Treatment of Wastewater of Dyeing baths Effluents in an Environmentally Safe Manner

Microbial Study with Mathematical and Statistical Modelling for Synchronous Biotreatment of Actual Restaurant Wastewater and Bioelectricity Recovery in Microbial Fuel Cell

Microbial Study with Mathematical and Statistical Modelling for Synchronous Biotreatment of Actual Restaurant Wastewater and Bioelectricity Recovery in Microbial Fuel Cell

Pollution Load Reduction from Domestic Wastewater with Electrocoagulation Process for Agricultural Reuse

Objectives: The present study is aimed to investigate electrocoagulation of domestic wastewater and assessment of pollutants removal efficiency for potential reuse in agriculture. Methods: The electrocoagulation treatment of domestic wastewater with Fe – Fe electrodes was performed under optimal conditions of pH (8.0), current density (0.6 mA/cm2), treatment time (45 minutes), and NaCl dose (2.8 g/L) in a slurry type of reactor. The primary clarified and biotreated domestic wastewaters were subjected to electrocoagulation with Direct Current (DC) as power source. Findings: There was observed higher pollutants removal efficiency from the biotreated wastewater as compared with the primary clarified wastewater after electrocoagulation. The treated wastewaters showed significant removal of pollutants in terms of BOD (79.5% – 87.9%), COD (86.8% – 89.5%), TDS (87.4% – 89.9%), TSS (66.7% – 75.3%), conductivity (77.8% – 78.4%), turbidity (74% – 81.2%), colour (77.7% – 86.2%), nitrates (44.1% – 51.7%), and phosphates (48.7% – 55.9%) after electrocoagulation treatment. Electrocoagulation considerably improved the biodegradability index of the primary clarified (0.59 to 0.92) and biotreated (0.69 to 0.8) wastewaters. This indicates easy removal of the pollutants further by biological processes in the aquatic ecosystems. Electrocoagulation demonstrated potential for removal of pollution from the domestic wastewater for productive reuse in agriculture and urban areas. Novelty: There exist few studies of use of electrochemical process for treatment and reuse of domestic wastewater. The treated waters complied with the regulatory standards and had satisfactory quality for reuse in agriculture and urban activities. Keywords: Electrocoagulation, Domestic wastewater, Biodegradability index, Agricultural reuse, National Green Tribunal

A panoramic view of the virosphere in three wastewater treatment plants by integrating viral-like particle-concentrated and traditional non-concentrated metagenomic approaches.

Wastewater biotreatment systems harbor a rich diversity of microorganisms, and the effectiveness of biotreatment systems largely depends on the activity of these microorganisms. Specifically, viruses play a crucial role in altering microbial behavior and metabolic processes throughout their infection phases, an aspect that has recently attracted considerable interest. Two metagenomic approaches, viral-like particle-concentrated (VPC, representing free viral-like particles) and non-concentrated (NC, representing the cellular fraction), were employed to assess their efficacy in revealing virome characteristics, including taxonomy, diversity, host interactions, lifestyle, dynamics, and functional genes across processing units of three wastewater treatment plants (WWTPs). Our findings indicate that each approach offers unique insights into the viral community and functional composition. Their combined use proved effective in elucidating WWTP viromes. We identified nearly 50,000viral contigs, with Cressdnaviricota and Uroviricota being the predominant phyla in the VPC and NC fractions, respectively. Notably, two pathogenic viral families, Asfarviridae and Adenoviridae, were commonly found in these WWTPs. We also observed significant differences in the viromes of WWTPs processing different types of wastewater. Additionally, various phage-derived auxiliary metabolic genes (AMGs) were active at the RNA level, contributing to the metabolism of the microbial community, particularly in carbon, sulfur, and phosphorus cycling. Moreover, we identified 29 virus-carried antibiotic resistance genes (ARGs) with potential for host transfer, highlighting the role of viruses in spreading ARGs in the environment. Overall, this study provides a detailed and integrated view of the virosphere in three WWTPs through the application of VPC and NC metagenomic approaches. Our findings enhance the understanding of viral communities, offering valuable insights for optimizing the operation and regulation of wastewater treatment systems.

Synchronous in-situ sludge reduction and enhanced denitrification through improving electron transfer during endogenous metabolisms with Fe(Ⅱ) addition

The creation of large amounts of excess sludge and residual nitrogen are critical issues in wastewater biotreatment. This study introduced Fe(II) into an oligotrophic anaerobic reactor (OARFe) that was implemented to modify an anoxic-oxic process to motivate in-situ sludge reduction and enhance denitrification under an effective electron shuttle among organic matter, nitrogen, and Fe. The addition of 15 mg L−1 Fe(II) resulted in a sludge reduction efficiency reached 32.0% with a decreased effluent nitrate concentration of 33.3%. This was mostly attributed to the electron transfer from Fe(II) to organic matters and nitrogen species in OARFe. The participation of Fe(II) led to the upregulation of Geothrix and Terrimonas, which caused active organic matter hydrolysis and cell lysis to stimulate the release of extracellular polymeric substances (EPS) and substance transfer between each layer of EPS. The higher utilization of released bioavailable dissolved organic matter improved endogenous denitrification, which can be combined with iron autotrophic denitrification to realize multiple electron donor-based nitrogen removal pathways, resulting in an increased nitrate removal rate of 58.2% in the absence of external carbon sources. These functional bacteria associated with the transformation of nitrogen and carbon and cycling between ferrous and ferric ions were enriched in OARFe, which contributed to efficient electron transport occurred both inside and outside the cell and increased 2,3,5-triphenyltetrazolium chloride electronic transport system activity by 46.9%. This contributed to the potential operational costs of chemical addition and sludge disposal of Fe-AO being 1.9 times lower than those of conventional A2O processes. These results imply that the addition of ferrous ions to an oligotrophic anaerobic zone for wastewater treatment has the potential for low-cost pollution control.

Occurrence, source apportionment and risk assessment of selected pharmaceuticals and their transformation products in the effluent-impacted rivers

The presence and risk of pharmaceutically active compounds (PhACs) in the aquatic environment have received global attentions recently, while the information about their transformation products (TPs) is limited. In this study, the occurrence and behavior of 40 common PhACs and TPs belong to 10 categories were investigated in the surface water of Ningbo city in China for two consecutive years. A total of 23 PhACs and TPs were detected among 20 sampling sites with the overall concentrations in the range of 77.7–742.8 ng/L. Source apportionment found that about 70% of the PhACs originated from the discharge of WWTP effluent. Caffeine and TPs 10,11-dihydroxycarbamazepine were typical indicators to differentiate unbiotreated and biotreated wastewater. CFI and fluoxetine were the pollutants with the highest risk value. The results indicated that the hydrodynamic conditions of rivers should also be considered to promote the biotic and abiotic transformation of PhACs besides pollution source control.

Effect of UV pretreatment on the source control of floR during subsequent biotreatment of florfenicol wastewater.

UV photolysis has been recommended as an alternative pretreatment method for the elimination of antibacterial activity of antibiotics against the indicator strain, but the pretreated antibiotic intermediates might not lose their potential to induce antibiotic resistance genes (ARGs) proliferation during subsequent biotreatment processes. The presence of florfenicol (FLO) in wastewater seriously inhibits the metabolic performance of anaerobic sludge microorganisms, especially the positive correlation between UV irradiation doses and ATP content, while it did not significantly affect the organics utilization ability and protein biosynthetic process of aerobic microorganisms. After sufficient UV pretreatment, the relative abundances of floR from genomic or plasmid DNA in subsequent aerobic and anaerobic biotreatment processes both decreased by two orders of magnitude, maintained at the level of the groups without FLO selective pressure. Meanwhile, the abundances of floR under anaerobic condition were always lower than that under aerobic condition, suggesting that anaerobic biotreatment systems might be more suitable for the effective control of target ARGs. The higher abundance of floR in plasmid DNA than in genome also indicated that the potential transmission risk of mobile ARGs should not be ignored. In addition, the relative abundance of intI1 was positively correlated with floR in its corresponding genomic or plasmid DNA (p < 0.05), which also increased the potential horizontal transfer risk of target ARGs. This study provides new insights into the effect of preferential UV photolysis as a pretreatment method for the enhancement of metabolic performance and source control of target ARGs in subsequent biotreatment processes. KEY POINTS: • Sufficient UV photolytic pretreatment efficiently controlled the abundance of floR • A synchronous decrease in abundance of intI1 reduced the risk of horizontal transfer • An appreciable abundance of floR in plasmid DNA was a potential source of total ARGs.

Biotreatment of swine wastewater by mixotrophic Galdieria sulphuraria

Biotreatment of swine wastewater via microalgae is an environmentally sustainable and economical efficient strategy capable to transform nitrogen, phosphorous, and organic/inorganic carbons into value added products as lipids, carbohydrates, and proteins, while high ammonium tolerant microalgal species with efficient COD and ammonium removal efficiency is lacking, limiting its practical application. Thermophilic and acidophilic Galdieria sulphuraria (G. sulphuraria) are reported to have high ammonium removal efficiency, while its application in ammonium rich swine wastewater treatment without dilution is rarely reported. Accordingly, mixotrophic cultivation of G. sulphuraria has been tested for biotreatment of swine wastewater. The results indicate that G. sulphuraria can grow well in swine wastewater with maximum growth rate of 0.72 g/(L.d) when 15 g/L of glucose is added. Simultaneously, a maximum COD removal efficiency of 94.8% and ammonium removal rate of 550.5 mg/(L.d) are observed. The addition of glucose leads to decreases of lipid, protein and ash contents, and simultaneous increase of carbohydrates owing to the varied C/N ratios. The final microalgal biomass has relatively lower carbon content of 48.5% with 20 g/L glucose than 49.3% of control, consequently leads to a relatively lower HHV value of 20.64 MJ.kg−1. Above all, mixotrophic cultivation of G. sulphuraria are promising strategy for swine wastewater treatment with high energy recovery, potentially providing an economical strategy for swine wastewater treatment.

Correlations between microbial taxonomies and wastewater quality parameters in a full-scale petroleum refinery wastewater treatment plant

In this study, we investigated the correlations between microbial taxonomies and wastewater properties in a full-scale petroleum refinery wastewater biotreatment system, comprising one anoxic tank and two aerobic tanks. Over one hundred organic compounds, mostly aromatics, were detected in the anoxic tank influent, with dominants being cresols and anilines. While the biotreatment process effectively removed most organic components, n-alkanes remained in the effluent of the second aerobic tank. Thauera and Ignavibacterium were the dominant genera across all tanks. Betaproteobacteria substantially decreased in the second aerobic tank where the aromatic compounds were depleted. The correlation network displayed two distinct microbial clusters. Genera in cluster I were generally positively correlated with each other and tended to co-occur, while genera in cluster II were subdivided into two small groups that were negatively correlated with the other group members. Mantel test showed that pH, nitrite and n-alkanes were potential determining factors influencing the overall community assembly. This study provides valuable insights into the chemical changes and microbial taxonomy in a complex petroleum refinery wastewater treatment system, establishing a foundation for further studies on the community regulation strategies within similar biotreatment systems.

Deciphering Microbe-Mediated Dissolved Organic Matter Reactome in Wastewater Treatment Plants Using Directed Paired Mass Distance.

Understanding the reaction mechanism of dissolved organic matter (DOM) during wastewater biotreatment is crucial for optimal DOM control. Here, we develop a directed paired mass distance (dPMD) method that constructs a molecular network displaying the reaction pathways of DOM. It couples direction inference and PMD analysis to extract the substrate-product relationships and delta masses of potentially paired reactants directly from sequential mass spectrometry data without formula assignment. Using this method, we analyze the influent and effluent samples from the bioprocesses of 12 wastewater treatment plants (WWTPs) and build a dPMD network to characterize the core reactome of DOM. The network shows that the first step of the transformation triggers reaction cascades that diversify the DOM, but the highly overlapped subsequent reaction pathways result in similar effluent DOM compositions across WWTPs despite varied influents. Mass changes exhibit consistent gain/loss preferences (e.g., +3.995 and -16.031) but different occurrences across WWTPs. Combined with genome-centric metatranscriptomics, we reveal the associations among dPMDs, enzymes, and microbes. Most enzymes are involved in oxygenation, (de)hydrogenation, demethylation, and hydration-related reactions but with different target substrates and expressed by various taxa, as exemplified by Proteobacteria, Actinobacteria, and Nitrospirae. Therefore, a functionally diverse community is pivotal for advanced DOM degradation.

Biotreatment of Industrial Wastewater using Microalgae: A Tool for a Sustainable Bioeconomy.

Fresh water is one of the essential sources of life, and its requirement has increased in the past years due to population growth and industrialization. Industries use huge quantities of fresh water for their processes, and generate high quantities of wastewater rich in organic matter, nitrates, and phosphates. These effluents have contaminated the freshwater sources and there is a need to recycle this wastewater in an ecologically harmless manner. Microalgae use the nutrients in the wastewater as a medium for growth and the biomass produced are rich in nutrition that can cater growing food and energy needs. The primary and secondary metabolites of microalgae are utilized as biofuel and as active ingredients in cosmetics, animal feed, therapeutics, and pharmaceutical products. In this review, we explore food processing industries like dairy, meat, aquaculture, breweries, and their wastewater for the microalgal growth. Current treatment methods are expensive and energy demanding, which indirectly leads to higher greenhouse gas emissions. Microalgae acts as a potential biotreatment tool and mitigates carbon dioxide due to their high photosynthetic efficiency. This review aims to address the need to recycle wastewater generated from such industries and potentiality to use microalgae for biotreatment. This will help to build a circular bioeconomy by using wastewater as a valuable resource to produce valuable products.

Exploration of Microalgae-Activated Sludge Growth Performance in Lab-Scale Photobioreactors under Outdoor Environmental Conditions for Wastewater Biotreatment

Increasing the volume of untreated and inadequately treated municipal wastewater undermines the circular economy potential of wastewater resources, particularly in low-income regions. This present study focused on and evaluated the performance of native microalgae-activated sludge (MAS) growth for tertiary treatment of anaerobically digested wastewater from an up-flow anaerobic sludge blanket (UASB) in an outdoor lab-scale photobioreactor (2.2 L). Three conditions with distinct MAS inoculum concentrations alongside three controls were operated in batch mode for 5 days hydraulic retention time (HRT) at 11.5:12.5 photo-hours. The MAS inoculum concentration influenced the treatment outcome. The best performance was observed when the MAS concentration was 0.10/0.20 g L−1, and the cell density was 1.60 × 107 cells mL−1, total biomass productivity of 0.10 g TSS L−1 d−1, total phosphorus uptake of 85.1%, and total nitrogen uptake of 66.1%. Logarithmic removal (Log-Re) of bacterial pathogens (water quality indicators) showed Log-Re 3.4 for total coliforms (1.37 × 102 CFU 100 mL−1) and 4.7 for Escherichia coli (0.00 × 100 CFU 100 mL−1). The results revealed optimum remediation performance and nutrient recovery potential with appropriate inoculum concentration, in admiration to advancing the science of circular economy.