Background: Treatment for diabetes is traditionally performed using subcutaneous insulin injection and oral anti-diabetic agents, especially plant-derived hypoglycemic agents. Besides, bioresonance therapy or biophysical information therapy is also performed for diabetes and other disorders as a form of complementary medical method. This study aimed to explore a specific hypoglycemic frequency of human insulin in microwave band and to assess the effects of irradiation of microwave at the frequency on blood glucose (BG) levels in normal and diabetic rats.