Eight types of Sudanese honey produced by Apis mellifera collected from Tabga region, River Nile State (North Central Sudan) of different floral sources, namely, Faseolus vulgaris " Fasoulia" , Balanites aegyptiaca" Hejleeg" , Acacia seyal var. seyal" Taleh Ahmr" , Faidherbia albida" Haraz" , Lawsonia inermis" Henna" , Tamarix nilotica" Tarfa" , Acacia seyal var. fistula" Taheh Abiyad" , and Acacia tortilis" Samur" were assayed for antibacterial activities against five clinical isolates bacteria associated with venereal diseases. These isolated bacteria include: Staphylococcus aureus, Escherichia coli, Klebsilla sp., Pseudomonas aeruginosa and Proteus sp. The antibacterial activity was determined by the method of Minimum Inhibitory Concentration (MIC). The study shows that the sensitivity of bacterial strains varies depending on the botanical source of the honey. The Minimum Inhibitory Concentration of honey ranged from <5 to >20 mg/ml." Fasoulia" ," Hejleeg" and" Taleh" honeys showed the highest activity against all tested bacteria. The antibacterial activity recorded for most honey samples used in the present study and the known safe use of honey without toxic effects suggest that honey could be used to treat venereal infections in a cheap and effective manner. Results of the present study also suggest a fairly good correlation between traditional therapeutic use and the in vitro antibacterial activity. These results corroborate the importance of ethnomedical surveys for screening honeys as a potential source of bioactive compounds. Hence these could result in discovery of novel antibacterial agents. Further bio-guided fractionation will be conducted for and to identify the active compounds.