Volume Of Biogas Research Articles

Evaluation of the efficiency of dry anaerobic digester in the production of biogas and fertilizer using activated sludge and plant waste.

The process of dry anaerobic digestion (DAD) is an effective economic and environmentally friendly method due to its features such as reducing the volume of waste, destroying pathogens, consuming less energy, and producing biogas rich in energy and fertilizer. This study was conducted on a pilot scale with a reactor volume of 24L in 30 days for three ratios of C/N: 30, 25, and 20 separately using a mixture of activated sludge and plant waste. During the process, the temperature, pH, and ratio of C/N were measured at certain times, and the percentage of gases produced by the Gas chromatography (GC) device was also measured. The results were compared with the National standard of Iran (Compost, Physical, and Chemical Specifications) at the end of the process. The results showed that the average volume of biogas produced in ratios of C/N: 30, 25, 20 is 1.7, 2.5, and 1.5L. d-1, respectively. The results showed that in the C/N ratio of 20, and 25, the amount of, C/N ratio and NO3 are in the range the standard of class 1, and the amount of Phosphorus pentoxide (P2O5) is in the range the standard of class 2. The results showed that the average microbial parameters in ratios of C/N: 30, 25, and 20 are lower than the national standard of Iran. The dry digester is an efficient method in waste treatment, methane gas production, and quality fertilizer close to the national standard of Iran.

Effect of varying volumes of anaerobic microbial inoculum on biodegradation and biogas production from black water

Unfettered discharge of untreated black water is one of the raging environmental issues which needs to be addressed especially in many developing and third-world countries. Black water is rich in organic compounds, elements such as nitrogen and phosphorous, and harmful faecal coliforms which makes it a major threat to the environment. The present study focuses on the use of an inhouse anaerobic microbial inoculum for the treatment of black water with an emphasis on decentralized treatment systems. The effects of varying volumes of inoculum (5 %, 10 %, 20 % & 30 %) for the treatment of black water was studied. Various parameters such as pH, total solids (TS), volatile solids (VS), chemical oxygen demand (COD) and biogas production were recorded. 10 % inoculum yielded the maximum amount of biogas. The biogas volume varied from 1046 mL–1180 mL depending on the experiments carried out using either fresh inoculum or acclimatized old inoculum. The inoculum could also be reused for sustained biodegradation and biogas production process which makes its use economic and apt as inoculum for reactors used in decentralized treatment processes. An attempt was also made in this work to reduce the input COD imparted via the inoculum itself by letting the larger and heavier solids to settle. Nevertheless, it was observed that the best working inoculum was the 10 % whole inoculum in terms of biogas production and COD removal.

Low-energy advanced wastewater treatment using submerged nanofiltration

The use of submerged nanofiltration (NF) membranes in wastewater treatment is an emerging process that produces high-quality recycled water with low energy consumption. This study aimed to identify the potential of coupling anaerobic digestion with this low-energy advanced wastewater treatment using submerged NF membranes. The direct NF of primary wastewater effluent using four types of commercial NF membranes resulted in progressive membrane fouling over 4 d. However, almost all the foulants contributing to hydraulic loss were removed by one-swipe sponge cleaning. This indicated that the major foulants were generally deposited on the membrane surface during direct NF. The membrane fouling speed varied during the long-term test depending on the feed wastewater quality. Although the transmembrane pressure increased by up to 50 kPa in every filtration cycle (4–7 d), sponge cleaning removed all the membrane foulants, resulting in >30 d of operation without chemical cleaning. The NF concentrates (four times concentrated wastewater) were then used for biogas production in an anaerobic digester. The volume of biogas formed from the NF concentrates sufficiently compensated for the energy consumption of direct NF. This study realized a low-energy advanced wastewater treatment utilizing NF concentrates that can also be used for biogas formation.

Bio-valorization of Cow Dung for Green Renewable Energy

Recovering organic waste, mainly animal manure, to produce biogas (through bio-methanization) could be seen as an economical, decentralized and environmentally friendly solution for farm waste management since it provides energy autonomy and sustainable agricultural development in rural areas. In this work, cow manure from a cattle farm located in Bouzereah, was used as a substrate. An experimental set up consists of 1.5-liter glass batch digester with a total solid concentration of 5.15%, a heating system (thermostated bath) and a magnetic stirring system. The experiment was monitored for 36 days in a thermophilic environment (55°C). The monitored physico-chemical characteristics of cow dung samples; are: pH, acidity, alkalinity, biogas volume and chemical oxygen demand (COD). Biogas production began at a rate of 0.27 L/day on day 11, with a total biogas potential over 50 L/kg of dried solid. The observed buffering capacity indicate a promising potential to use this substrate in co-digestion process namely for acidic substrate such as chees-whey and household wastes.

Influence of Molasses and Caesalpinia spinosa Meal Inoculums on Biogas Production from Cattle Manure

The management of organic waste through anaerobic digestion is an alternative to energy recovery. This research focused on evaluating the production of biogas with different inoculums. For this purpose, two types of systems were implemented—one used a heating system controlled by an STC-1000 thermostat, while the other used a solar heating system under a polycarbonate parabolic trough. The experiment was carried out at laboratory level with 3 L PET bottle biodigesters and the biogas produced was collected with the water displacement technique in 3 L bottles, calibrated every 50 mL, over 43 days. Inoculums of the following manure concentrations were used: water (1:5, 1:2, 1:3) mixed with Caesalpinia spinosa meal and molasses. The results determined that the thermostat-controlled heating system generated 69.6 mL/day of biogas while the other system produced 610.9 mL/day. On the other hand, the T1 treatment with a manure:water ratio of 1:5 and molasses and Caesalpinia spinosa meal inoculums in both systems had a higher average biogas volume. In terms of methane (CH₄), the highest value of 76.9% was obtained through the T1 treatment under the controlled heating system. This allows the production of biogas with a high concentration of methane, which in future applications can be utilized for residential or industrial purposes, promoting economic, social and environmental development. Since the main challenge in the production of biogas is to reduce the digestion time, which is influenced by the temperature of the site, two types of inoculums with a low cost and easy access were used.

Co-digestion of Sewage Sludge and Biowaste for Biogas Production, GHG Avoided Emissions, and Profitable Carbon Credit Development

In Estonia, where biowaste comprises a significant portion of municipal waste, efficient waste management strategies are essential. Co-digestion presents a promising avenue to enhance biogas yield and reduce greenhouse gas emissions. This study explores the potential of co-digesting sewage sludge and biowaste for biogas production, emphasizing carbon credit calculation and offset project development. Sewage sludge was effectively utilized as a co-feedstock for the co-digestion process, contributing to increased biogas production. In Narva City, 20,401.08037 m3 /year of biogas was produced in 2012, facilitating the generation of electricity from renewable sources and thus reducing GHG emissions, which facilitates the calculation of carbon credits. We developed a robust carbon offset project based on the biogas volume, meeting additionality criteria and demonstrating long-term benefits. The revenue potential from carbon credits ranged from 118 EUR to 41300 EUR, depending on market prices and project attributes. Moreover, the offset project and calculated carbon credits offer tangible benefits to sustainable waste management and the implementation of the circular economy. By valorizing sewage sludge and biowaste through anaerobic digestion, the project contributes to waste diversion from landfills, reduces methane emissions, and promotes renewable energy generation. This integrated approach aligns with principles of sustainable waste management and supports the transition towards a circular economy model. Our findings provide valuable insights for policymakers and stakeholders interested in leveraging anaerobic digestion for renewable energy production and carbon mitigation.

Optimization of the Factors Affecting Biogas Production Using the Taguchi Design of Experiment Method

The present study analyzed the effect of temperature, pH, pre-treatment and mixing ratio on the anaerobic digestion process. The parameters during the anaerobic co-digestion of cow manure and food waste were then optimized using the Taguchi experimental design method. ANOVA was carried out to find the significant parameters which influence biogas production. Experimental tests were carried out at laboratory-scale reactors kept at different temperatures (28 °C, 35 °C, and 50 °C). The specific methanogenic performance (SMP) during anaerobic digestion at higher temperatures was characterized with the analysis of acetate, propionate, butyrate, hydrogen, glucose, and formate, and was validated with the literature. The improvement of biogas production with different pre-treatments, i.e., ultrasonic, autoclave, and microwave techniques, was also analyzed. The results showed that the reactor that was maintained at 35 °C showed the highest biogas production, while the reactor that was maintained at a lower temperature (28 °C) produced the lower volume of biogas. As the retention time increases, the amount of biogas production increases. Methanogenic activities of microorganisms were reduced at higher temperature conditions (65 °C). Biogas production increased by 28.1%, 20.23%, and 13.27% when the substrates were treated with ultrasonic, autoclave, and microwave, respectively, compared to the untreated substrate. The optimized condition for the highest biogas production during anaerobic co-digestion of food waste and cow manure is a temperature of 35 °C, a pH of 7 and a mixing ratio (CM:FW = 1.5:0.5). ANOVA showed that temperature is the most important input parameter affecting biogas production, followed by mixing ratio.

The effect of raw material (cow and chicken manure) and reactor type for improving and maximizing biogas production.

Biomass energy is a type of renewable energy and animal waste is one of the main resources for its production. The purpose of this study is to investigate the effect of raw material type (cow and chicken manure) and the type of reactor (digester) on the biogas produced by measuring the amount of methane in the product. Three types of digester (metal, simple PVC, and PVC with leachate rotation) with the same volume (10 L) were prepared. Equipment was installed on the digesters to measure the pH and volume of produced gas. The experiments were carried out in controlled temperature conditions (28-30 °C) and in two stages. The first experiment was to load the digesters with cow excrement, and the second experiment was to load the digesters with chicken excrement. In both experiments, the digesters were fed with 1.5 kg of animal manure and water with a ratio of 1:1. During a period of 60 days, the volume of biogas and methane produced was measured and recorded. The results showed that the amount of biogas produced from chicken waste is more than the amount obtained from cow waste. However, the amount of methane produced using cow excrement was more than that of chicken excrement. Also, the performance of PVC digester with leachate rotation was better than the other two digesters, which could be due to the mixing of raw materials in this type of digester.

Study of the methanogenic potential of used grease and bleaching earth by conversion into biogas: The case of a wastewater treatment plant and a refinery

The structure from which this study was carried out is a food processing company specializing in the transformation of crude palm oil into table oil. In order to comply with environmental standards when discharging the refinery's wastewater, it has its own wastewater treatment unit. The aim of this study is to contribute to environmental protection by recovering grease and bleaching earth through biogas production by anaerobic digestion. The characterization provided us with moisture, dry matter and volatile solids contents the values obtained are 32.31%, 67.69% and 97.57% respectively. In the same order, those for bleaching earth are 1.80%, 98.20% and 59.97%. The values obtained during the characterization of the bleaching earth are not conducive to good anaerobic digestion. To optimise biogas production from fat, we used inoculums such as cattle dung and broiler droppings. Biodegradability tests carried out with different proportions of substrates and inoculums led to the conclusion that the presence of a large quantity of microorganisms is necessary for optimum biogas production. In addition, the grease produced a good quantity of biogas when co-digested with the inoculums. The cumulative volume of biogas obtained over 30 days with the grease was 445 ml. The highest quantity of biogas obtained by optimising gas production is 780 ml.

Biogas production from water hyacinth (Eichhornia crassipes) harvested from River Wouri, Douala, Cameroon

Over the past three decades, growing global interest has been in replacing fossil fuels with clean energy from renewable resources. As a result, biogas generation from biodegradable organic waste has become increasingly popular, and water hyacinth is a menace wherever it exists. This study aimed to assess the effectiveness of water hyacinth (Eichhornia crassipes) from River Wouri as a biogas substrate. Equal volumes (700 mL) of water hyacinth and poultry-based inoculum were prepared, each with a duplicate. The 8 substrates were placed in 1-liter plastic and incubated in a water bath at 37± 2 °C for 106 days. The substrates were subsampled before and after incubation to analyse the dry matter, moisture, volatile solids, ash contents, and pH. The volume of biogas produced from each digester was collected in plastic bags and measured every three days; hence, the methane produced was calculated. Results indicated an increase in the moisture contents of the substrates, while pH levels remained unchanged after incubation. The rate of destruction of volatile solids increased with the level of added inoculum. Biogas production started almost immediately for all the samples and largely followed a dome shape over the incubation period. Cumulative methane produced were 10.23, 11.84, 5.88, and 0.71 LCH4/kgVS, respectively, for 100% water hyacinth, 90% water hyacinth, 80% water hyacinth and 0% water hyacinth. This study demonstrates the effectiveness of the weed in biogas production, which could be an important method to manage River Wouri while generating renewable energy.

Optimization of the biofuel production by idealized fermentation of the animal manure, chicken wastes, and sewage sludge

Abstract This study aims to optimize an economic procedure to produce biogas and bio-ethanol from different organic wastes such as sewage sludge (SS) and/or cattle dung (CD) and/or poultry manure (PM). The experiment was carried out at a wastewater treatment plant in Egypt. Each waste type was mixed with the starter, CaCO3, and water then loaded in a fermenter and kept for 35 days at 35 °C under the anaerobic digestion. The evolved volume of the biogas and the content of methane CH4 were measured daily while the cellulase and protease enzymes were tested every four days. Results have indicated that the digester containing the SS has produced the greatest biogas volume (L) 27.45 Lb/D/d (liters biogas/digester/day), 0.61 Lb/D contents’ volume/d, and cumulative 606.30 Lb/D during the 16th day. Significant CH4 volume percentages produced during the 17th day were 72.07, 71.16, and 71.11% while the produced bio-ethanol alcohol was 2.47, 2.32, and 1.99% from the SS, CD, and PM, respectively. The procedure efficiency is prominent by the production of the biogases and in-situ activating enzymes all in one reactor that was periodically monitored for its reactants and product content. No need for the pre-treatment of wastes as raw materials or chemical additives and the fermented residue can be further tested for soil fertilization. These wastes can be promising for bio-energy production being economic and environment friendly.

Magnetic iron oxide nanoparticle enhanced microwave pretreatment for anaerobic digestion of meat industry sludge

Our study investigates the effects of iron oxide (Fe3O4) nanoparticles combined microwave pretreatment on the anaerobic digestibility and soluble chemical oxygen demand (SCOD) of meat industry sludge. One of our main objectives was to see whether the different microwave-based pretreatment procedures can enhance biogas production by improving the biological availability of organic compounds. Results demonstrated that combining microwave irradiation with magnetic iron oxide nanoparticles considerably increased SCOD (enhancement ratio was above 1.5), the rate of specific biogas production, and the total cumulative specific biogas volume (more than a threefold increment), while having no negative effect on the biomethane content. Furthermore, the assessment of the sludge samples’ dielectric properties (dielectric constant and loss factor measured at the frequency of 500 MHz) showed a strong correlation with SCOD changes (r = 0.9942, R2 = 0.99), offering a novel method to evaluate pretreatment efficiency.

METHANE PRODUCTION FROM COW MANURE IN THE PRESENCE OF Bi(III) COMPLEX OF SULFAMIC ACID

The present study examines the process of biomethanization of cow manure in an anaerobic environment, in the presence of small amounts of Bi(III) complex of sulfamic acid. Since this complex has good antimicrobial activity, the purpose of adding the complex is to determine its effect on biogas and methane production. The reference samples (cow manure only) and mixtures of cow manure + 10 mg and cow manure + 20 mg of bismuth complex with a composition [Bi6O4(OH)4](NH2SO3)6 are subjected to biodegradation under the following conditions – duration of 33 days, nitrogen atmosphere and temperature of 34.5 ± 1°C. During the process, the volume of biogas and the percentage content of methane in biogas are measured in 2 - 3 days as well. The analyses performed reveal that until the 10th day, the amount of biogas produced increases constantly and is comparable both for the control samples and for the mixtures. Further, until the end of the experiment, a significant and constant increase in the volume of biogas produced by the mixtures is observed compared to the control samples.Regarding the percentage of methane produced by the reference samples and the mixtures, comparable relative amounts are found by the 7th day. From the 8th day to the 33rd day, the percentage of methane released by the mixtures is visibly greater than that by the reference samples.

Optimizing the ratio of raw materials and pH on biogas production from cow manure with banana peel waste in batch system reactors

Politeknik Negeri Jember has dairy cows TEFA which produce relatively large amounts of cow dung waste, namely 280 kg per day. The fecal waste is directly thrown into waterways, causing air pollution, namely causing an unpleasant odor. It would be interesting to conduct research on cow dung waste in TEFA of dairy cows. This research was carried out with the aim of utilizing cow manure which is waste in TEFA dairy cattle as raw material for biogas with a mixture of banana peel waste as a substrate. In this research, a batch system reactor with an anaerobic fermentation method was used. The variables used were the ratio of cow manure to banana peel waste with variations of 1:1, 1:2, 1:3, and the pH variable variations of 6, 7, and 8. The test parameter observed in the research was the volume of biogas. The experimental design used to determine optimum conditions is the Response Surface Method (RSM). The experimental design was carried out on 2 variables with 3 variations so that 9 data points were obtained. Based on the test results, it is known that the optimum conditions for the variable ratio of cow dung to banana peel waste and pH are 1:3 and the pH variation is 6, where the volume of gas produced is 177.8 ml.

Tofu Liquid Waste Treatment Using Effective Volume of Anaerobic Sequencing Batch Reactor (ASBR)

The tofu industry produces liquid waste containing high organic compounds. Organic compounds in liquid waste can threaten aquatic ecosystems if discharged directly into water bodies. One of the most effective wastewater treatment systems is the treatment using Anaerobic Sequencing Batch Reactor (ASBR). Besides being able to reduce the content of organic compounds, ASBR can produce biogas, and its decomposing microorganisms do not flow into the effluent stream. The purpose of this study was to evaluate the performance of ASBR at an effective reactor volume according to the design. This is because the use of ASBR in previous studies had not reached the operational stage and did not use an effective volume reactor. The stages of waste treatment using ASBR include the seeding, acclimatization, and operation stages. Waste treatment took place at room temperature in the reactor with an effective volume of 6 L. The operating parameters tested were MLVSS, COD, BOD, pH, and cumulative biogas volume. The results obtained show that the seeding process took 56 days, acclimatization took 10 days, and the operation lasted for 17 days. The efficiency of reducing COD concentration was 60%, the efficiency of reducing BOD concentration was 35.65%, and the cumulative volume of biogas produced was 24,120 mL at the operating stage. The use of the ASBR system at an effective volume of 6 L resulted in the successful processing of tofu liquid waste.

Analysis of Anaerobic Digestion Installation Testing for Tofu Liquid Waste Utilization into Biogas with the Addition of Cow Manure Variations

The purpose of this study is to convert tofu wastewater into biogas using an anaerobic digester with varying cow dung mixtures (10%, 15%, and 20%). The study method entails creating digesters including independent, dependent, and controlled variables. Variations in pH, temperature, biogas pressure, and biogas volume were detected throughout the course of a 15-day fermentation. The data show that in the 10% fluctuation, the greatest pH was on day 7 (7), whereas the highest temperature was 32°C on days 6 and 7. Biogas pressure emerged on day 6 (89.2 cmHg), resulting in a volume of 90 liters by day 15. In the 15% variation, the highest pH (7.2) occurred on day 8, with the highest temperature (34°C) on days 9 and 10. Biogas pressure began on day 5 (89.9 cmHg), with a biogas volume of 95 liters on day 15. The 20% variance resulted in the highest pH (7.4) on day 8 and the highest temperature of 35°C from days 9-11. Biogas pressure began on day 5 (90.7 cmHg), resulting in a biogas volume of 100 liters on day 15. This study sheds light on how to manage tofu waste into biogas with varying degrees of effectiveness and efficiency.

Analisis Pengaruh Rasio Jerami Dan Kotoran Sapi Pada Biodigester Terapung Pada Gas Yang Dihasilkan

To address the concerning trend of increasing fossil fuel use due to population growth, it is necessaryto prioritize alternative energy sources like renewable energy.This study aims to analyze the energy parameters produced through biogas formation in a floating drum biodigester, highlighting the potential for sustainable energy solutions.This research method uses experimental research using three treatments of the ratio of straw, water, and cow dung. The parameters observed and analyzed include the amount of biogas produced, biogas pressure, flame length, combustion rate, and calorific value.The experiment results clearly demonstrate that the first treatment was significantly more effective in producing biogas, with a volume of 0.101 m3 or 101 litre, a pressure of 0.0081 atm, a combustion rate of 0.00266 m3/min, a flame length of 38 minutes, and a combustion calorific value of 210,045 joules.In contrast, the second treatment produced a total biogas volume of only 0.0775 m3 or 77.5 litre, at a pressure of 0.0131 atm, a combustion rate of 0.00209 m3/min, a flame duration of 37 minutes, and a combustion calorific value of 184,965 joules. These results provide strong evidence for the superiority of the first treatment in terms of biogas production. The third treatment produced a biogas volume of 0.0771 m3 or 77.1 litre at a pressure of 0.013 atm, with a combustion rate of 0.00214 m3/min, a flame duration of 36 minutes, and a combustion heating value of 164,587.5 joules.

Continuous co-digestion of sewage sludge and highly concentrated waste bioplastic hydrolyzate without shortening hydraulic retention time

Bioplastics have garnered substantial interest as alternatives to conventional petroleum-based plastics. However, their management and conversion to biogas continues to be significantly challenging. In this study, we evaluated the suitability of various plastics for hydrolysis at 160 °C for 12 h using different solvents. The biogas yield (BGY) of the monomers constituting these plastics and the obtained plastic hydrolyzates was comprehensively evaluated. When water was used as a solvent, 100 % hydrolysis of polylactic acid (PLA) and polybutylene succinate (PBS) was observed. When polybutylene adipate co-terephthalate (PBAT) and polyhydroxybutyrate (PHB) were hydrolyzed with water, the degradation ratio was approximately 30 %; however, using an aqueous lactic acid solution as a solvent improved the degradation ratio to 78 % and 100 %, respectively. In the BGY test of the plastic hydrolyzates, the biogas volumes derived from the hydrolyzates were 563, 461, 337, and 573 mL/g COD-added for PLA, PBS, PBAT, and PHB, respectively. With 1,160 gCOD/L waste PLA hydrolyzate, continuous co-digestion of sewage sludge and the hydrolyzate was conducted. Organic loading rates of sewage sludge and the hydrolyzate were 2.3 and 2.4 gCOD/L/d, respectively. The operation was stable and the methane production volume from the PLA hydrolyzate was 414 L/kgCOD-added. Using highly concentrated PLA hydrolyzate, the hydraulic retention time was 19.3 days, which was only 0.7 days shorter than that of anaerobic digestion of sewage sludge only (20 days). Therefore, highly concentrated PLA hydrolyzate maintains the retention time of normal sewage sludge digestion. Conclusively, the present study has crucial practical implications for plastic waste management.

Thermodynamic analysis of anaerobic digestion of Eichornia crassipes (Water Hyacinth) biomass from the Volta River basin of Ghana using fruit waste sludge as inoculum

In order to gain understanding on the utilization of biogas fuel derived from water hyacinth (WH) biomass and render it appealing as a sustainable gaseous fuel, the study reports on its various thermophysical, combustion and thermodynamic parameters. The parameters include, specific enthalpy, internal energy, low heating value, Wobbe number index, changes in Enthalpy, Entropy and Gibbs free energy. The experimental procedure was performed at temperature range of 29±3 °C for 61 days in a BlueSens laboratory-scale anaerobic digestion equipment. It comprised of three fermenter bottles designated as F1, F2 and F3. F1 served as the control and the rest served as the test fermenter bottles. The digestion of water hyacinth and fruit waste sludge (WH+FWS) recorded the highest biogas volumes of 19,798.79 ml in fermenter F2 and 19,168.55 ml in fermenter F3 with methane contents of 53.46 % and 51.85 %, respectively. In comparison, the biogas produced exclusively from the water hyacinth (WH) biomass yielded 12,014.49 ml in F2 and 11,384.25 ml in F3, with methane contents of 46.41 % and 43.31 %, respectively. The best biogas fuel was produced from the digestion of (WH+FWS) in fermenter F2 with an internal energy and enthalpy values of 258.6 KJ/kg. K and 344.9 KJ/kg. K, respectively. A low heating value and Wobbe number of 17.51 MJ/m³ and 17.78 MJ/m³ were determined respectively. The biogas production was simulated using the Gompertz modified kinetic equation in MATLAB and an average specific rate (Kexp) of 371.49 ml/gVS.day was obtained. The rate constant (Kexp) was applied in the linearized form of the Erying-Polanyi equation to determine the changes in Enthalpy Entropy, and Gibbs free energy as -2510.66 J, -304.78 J and 94,219 J, respectively which indicated that, the anaerobic digestion process in this study was only thermodynamically feasible at low temperatures.

Isolation and Characterization of Microorganisms Involved in Biogas Production from Agricultural Waste

The study aimed to investigate the microbial composition involved in the biogas production process using a diverse range of substrates, including Spinacia oleracea (vegetable), banana peel, plant extract, watermelon residue, wheat straw and paddy straw, sourced from multiple locations in Jaunpur. Over a period of five weeks (30 days), the research employed established microbiological methodologies and customized anaerobic bio-digesters for the comprehensive analysis of the isolates and substrates to assess biogas generation. The evaluation revealed dynamic fluctuations in the digester temperature within the range of 30°C to 36°C, accompanied by initial pH levels ranging from 4.2 to 8.3, which subsequently decreased to pH 5-6 during and after the anaerobic digestion process. The identified anaerobic bacterial species encompassed Staphylococcus sp., Micrococcus, Enterobacter, Escherichia, Citrobacter, Bacillus sp., and Pseudomonas aeruginosa. Furthermore, the findings demonstrated a hierarchy in the percentage of biogas yield from the substrates, with the following ranking: synergistic mixture > plant extract > banana > wheat straw > spinach > watermelon. Notable disparity in the volume of biogas produced was observed across different substrate treatments and digestion periods. The research underscored the pivotal role of methanogens and other auxiliary bacteria in the overall biogas production process. Additionally, the average pH levels were determined to range between 6.3 - 7.2 before and 5.0 - 6.2 during and after anaerobic digestion. The observed decline in pH during the anaerobic digestion process was associated with the production of metabolites such as acetate, hydrogen gas, carbon dioxide and other volatile fatty acids, exerting significant influence on the substrates within the digesters.