The present study aimed to identify factors that are associated with puberty knowledge among school-going rural adolescents in Bangladesh. This cross-sectional study was conducted on 2724 school-going (grades VI-IX) adolescents who were aged between 10-24 years. The adolescents resided only in rural areas of Bangladesh. In this study, relationship between socio-demographic factors and controlling behaviour was assessed considering Bronfenbrenner's bioecological model. Considering the complex nature of Bronfenbrenner's bioecological model the structural equation model to explore factors related to the Adolescents' knowledge of pubertal changes. The structural equation model result showed a significant association among gender, education, age, and parental limit setting on daily activities with student's knowledge on pubertal changes. peer connection, and peer regulation were associated with adolescent knowledge on puberty directly as well as through the mediator variables year of schooling, academic performance and, parental behavioural control. Adolescents Age, years of schooling, and teachers concerns are positively associated with adolescents' knowledge on puberty. Whereas, parents' and peers' controlling behaviors are negatively associated with adolescents' understanding of pubertal changes. Therefore, there is needed an effective plan to raise the attention of parents and teachers on adolescents' pubertal issues to ensure adolescents' informed pubertal period.