Following activation, many receptors at the cell surface partition into clathrin coated pits (CCPs) which subsequently pinch off and move into the cell. Total internal reflection fluorescence (TIRF) microscopy has been used to study such events because it predominantly illuminates <70nm proximal to the coverslip, reducing background fluorescence from the depth of the cell. Loss of clathrin fluorescence from the evanescent field is typically interpreted as endocytosis; however alternative clathrin dynamics at the cell membrane, including formation of clathrin coated pits (CCPs) that are later aborted, has been described. Disappearance of clathrin in TIRF could represent movement of a CCP away from the membrane, uncoating of clathrin from a CCP, or dissociation of clathrin from the cell membrane. To differentiate between these possibilities, we simultaneously imaged a marker which remains with the vesicle. The epidermal growth factor receptor (EGFR) is activated by EGF binding, which induces uptake of its receptor into the cell. In Cos7 cells we analyzed EGFR containing CCPs using Epi/TIR imaging to visualize vesicles as they moved from the cell membrane to deeper in the cell (appearing only in Epi). This allowed us to identify the CCPs that were unambiguously undergoing endocytosis. We demonstrate the utility of EGFR as a model cargo for tracking endocytic vesicles. We tracked individual vesicles following endocytosis, and examined the dynamics of clathrin and EGFR relative to each other. This reveals a range of vesicle behavior as they enter the cell including variable dynamics of clathrin uncoating.