Binding Agents Research Articles

Conversion of Metal Pyrazolate/(Hydr)oxide Clusters into Nanojars: Solution vs Solid-State Structure and Magnetism.

Nanojars are a class of anion binding and extraction agents composed of a series of [Cu(μ-OH)(μ-pz)]n (pz = pyrazolate; n = 26-36) supramolecular metal-organic complexes. In contrast to other anion binding agents amenable to liquid-liquid extraction, nanojars only form by self-assembly around the target anion, and guest-free nanojar hosts cannot be isolated. An extraordinary binding strength toward highly hydrophilic anions such as carbonate and sulfate was demonstrated by the inability of Ba2+ ions to precipitate the corresponding insoluble barium salts from nanojars. Herein, we provide an additional proof for the superior robustness of the nanojar framework based on competition experiments with other transition metal pyrazolate/(hydr)oxide complexes. In addition to the mass spectrometric characterization, we present variable-temperature nuclear magnetic resonance studies with an emphasis on the influence of the paramagnetic Cu2+ centers on 1H hyperfine shifts, along with X-ray crystallographic analysis of two polymorphs of (MePh3P)2[CO3⊂{Cu(OH)(pz)}27], including the highest (cubic) symmetry nanojar crystal lattice obtained to date as well as magnetism studies for the first time. Furthermore, we provide evidence for the first molybdate-incarcerating nanojars, [MoO4⊂{Cu(μ-OH)(μ-pz)}n]2- (n = 28, 31-33), formed by rearrangement from [MoVI8O12(μ-O)9(μ-pz)6(pzH)6·3pzH] in the presence of Cu2+ ions.

Fabrication of binder-free TiO2 P 25 films on flexible PET/ITO substrate for photoanode in dye-sensitized solar cells

In this work, TiO2 P 25 films for photoanode in dye-sensitized solar cells (DSSCs) have been fabricated using a flexible PET/ITO substrate with a low-temperature heating technique. Ammonium hydroxide/ethanol solution was used as an alternative binding agent to fabricate TiO2 paste, which can be removed at low-temperature heating. The conventional screen print method is still used in DSSC fabrication because it is a very cheap and easy technique. A hot-pressing method is also used to improve the quality of TiO2 films and the performance of DSSCs. The encapsulation technique used in the assembly of DSSC is expected to increase the stability of DSSC devices to prevent electrolyte leakage. The resulting TiO2 film shows the highest DSSC performance using the hot-pressing and encapsulation method with PCE 0.29%. The encapsulation technique increased the stability of DSSC devices with more extended durability than without encapsulation.

Full correction of the self-absorption of laser-induced plasma beryllium emissions via sample preparation

Self-absorption effect is always encountered in laser-induced breakdown spectroscopy (LIBS) and immediately distorts the calibration curves especially for the elemental determination of complex materials. With the aim to provide an expeditious approach for LIBS measurements susceptible to self-absorption effect, an exploratory study for sample preparation by powder mixing is implemented, and the self-absorption of laser-induced plasma beryllium emissions is investigated as a function of the dilution factor. For comparison, boric acid and wax powder are separately used as typical binding agent to mix with beryl powder to produce two sets of pressed pellets with sequential gradient of beryllium content variation. For the resonant lines most prone to self-absorption of Be II emission doublet at 313.042 nm and 313.107 nm, the beryllium spectral line shapes can be directly regulated and improved in the case of both sets of pellets as the dilution factor increases. In addition, the self-absorption effect for the strongly emitting beryllium spectral lines is further assessed by calculating the self-absorption coefficients based on the radiation transport equation. With regard to the both types of binder as diluent, when the dilution factor for beryl increases to 10 with a weight percent of 0.386% for beryllium in the pellet, the SA values may exponentially increase in general to around 0.8 and eventually asymptotically approaches to 1 without exception. Thus the self-absorption effect of laser-induced beryl plasma emissions can be readily overcome with sample preparation by powder mixing. By contrast with laser pulse irradiance in the present work, the dilution factor for powder mixing plays a dominant role in eliminating self-absorption effect. This research proposes a potentially effective approach to reduce self-absorption in laser-induced plasma emissions.

Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity and neutralization sensitivity.

The human immunodeficiency virus (HIV) envelope protein (Env) mediates viral entry into host cells and is the primary target for the humoral immune response. Env is extensively glycosylated, and these glycans shield underlying epitopes from neutralizing antibodies. The glycosylation of Env is influenced by the type of host cell in which the virus is produced. Thus, HIV is distinctly glycosylated by CD4+ T cells, the major target cells, and macrophages. However, the specific differences in glycosylation between viruses produced in these cell types have not been explored at the molecular level. Moreover, it remains unclear whether the production of HIV in CD4+ T cells or macrophages affects the efficiency of viral spread and resistance to neutralization. To address these questions, we employed the simian immunodeficiency virus (SIV) model. Glycan analysis implied higher relative levels of oligomannose-type N-glycans in SIV from CD4+ T cells (T-SIV) compared to SIV from macrophages (M-SIV), and the complex-type N-glycans profiles seem to differ between the two viruses. Notably, M-SIV demonstrated greater infectivity than T-SIV, even when accounting for Env incorporation, suggesting that host cell-dependent factors influence infectivity. Further, M-SIV was more efficiently disseminated by HIV binding cellular lectins. We also evaluated the influence of cell type-dependent differences on SIV's vulnerability to carbohydrate binding agents (CBAs) and neutralizing antibodies. T-SIV demonstrated greater susceptibility to mannose-specific CBAs, possibly due to its elevated expression of oligomannose-type N-glycans. In contrast, M-SIV exhibited higher susceptibility to neutralizing sera in comparison to T-SIV. These findings underscore the importance of host cell-dependent attributes of SIV, such as glycosylation, in shaping both infectivity and the potential effectiveness of intervention strategies.

Preparation and Evaluation of Metronidazole Tablets Produced with Different Binders Using Wet Granulation Method

In tablet formulation, binders are essential for ensuring proper powder mix, granulation and overall tablet quality. Variations in binder quality and concentration can impact drug bioavailability, strength and manufacturing processes. The inconsistency in bioavailability and therapeutic response of metronidazole from different manufacturers highlights the importance of binder selection in tablet formulation. This study evaluates the impact of different binding agents on tablet quality. The optimum binder concentration for each of the binders was assessed to obtain suitable concentration that was used to prepare metronidazole tablets. Metronidazole tablets were formulated with four different binder vis, acacia, gelatine, PVP, maize starch at 3% concentration using wet granulation method after which the tablet properties were evaluated. Metronidazole powder exhibited poor flow indices, as indicated by angle of repose: 39.92 ± 1.85, Carr’s compressibility index (CI): 20.13 ± 0.28 and Hausner’s ratio (Hr): 1.26 ± 0.01, whereas its granules exhibited good flow properties. The crushing strength (CS), friability (FR), disintegration time (DT) (CSFR/DT) ratio for batches showed values in 3% binder concentration, indicating higher strength as compared to other concentrations. There was a significant increase (P < 0.05) in the CSFR/DT ratio for gelatine (2.22) in formulation F2 compared to maize starch in Formulation F4. The formulated tablets with the various binders have met with the British Pharmacopeial criteria of 80% drug release from uncoated immediate-release tablets. Amongst the four binders, gelatine was found to be the best in the formulation of robust metronidazole tablets by wet granulation method at an optimised concentration of 3% w/w. Tablets produced with gelatin exhibited greater mechanical strength than those produced with other binders, while also demonstrating a slightly longer DT. Metronidazole tablets produced with gelatine have met the British Pharmacopeial requirements including an acceptable in-vitro drug release profile.

Edaphological Description of the Type Habitat of Coptis' Teeta Wall. - an Endangered Species of Medicinal Importance in Arunachal Pradesh

The edaphic conditions of the habitat of Coptis tetla Wall. have been discussed. The soils, where this species grows either under cultivation or in wild are moderately acidic to highly acidic, poor in surface porosity, very rich in silica content and possess insufficient amount of inorganic binding agents. However, the soils in cultivated fields are satisfactory in organic carbon status, water-holding ability and cation exchange capacity but inadequate presence of liming material and temperate climate adversely effect their energy materials. The 3oils at Malenja where this species is growing wild have textural limitation and anthropogenic interference. In the end the paper is concluded with suggestions. Results depict that the reasons for rarity and depletion of this species are not only anthropogenic but also environmental.

Oxygen Atom from Carbonyl Group as an Important Binding Agent to the G-Quadruplex - Study Case of Flavonoids.

In the field of anticancer therapy study it is of great interest to find effective G-quadruplex ligands which may be of potential use in medical treatment or cancer prevention. Since among the compounds of natural origin, flavonoids have attracted notable attention because of their unique properties and promising therapeutic applications, an interesting question was to identify the flavonoid structural features that could provide effective binding properties toward G-quadruplex. By using electrospray ionization mass spectrometry, followed by the survival yield method, it has been shown that the flavonoid molecules which contain an available C4=O carbonyl group form more stable adducts with G-tetrads than the other ones. Molecular docking has shown that C4=O carbonyl group can be a source of hydrogen bonds and/or π-stacking interactions. Therefore, the flavonoid molecules which contain an available C4=O carbonyl group can be regarded as good binders of G-quadruplexes.

Development of biopellet formulation of Lecanicillium saksenae for the management of pepper root mealybug (Formicoccus polysperes)

Present study was carried out during 2020–23 in the Biocontrol Laboratory for Crop Pest Management, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala. An indigenous isolate of Lecanicillium saksenae (Kushwaha) Kurihara and Sukarno, ITCC 7714 was assessed for its effectiveness against the pepper root mealybug (Formicoccus polysperes Williams). Peculiar symptoms like sudden dissolution of mealy coating subsequent to the application of conidial suspension and paralysis occurring 12 h post-treatment was observed in mealybugs upon infection. Its high potency to mealybug was established with its low LC50 value of 2.2 × 105 conidia/ml and its quick killing ability was proved with a lethal time of 23.13 h. Of the various carriers tested for pelleting, talc + chitin (95:5) was found to be the best as it exhibited highest conidial viability of 78.54%, 5-weeks after storage. Other carriers such as talc, chitin, chitosan and talc + chitosan (90:10) exhibited conidial viability of 33.96–74.58%. Among the different binding agents examined, the combination of talc + chitin (95:5) + carboxymethyl cellulose 6% was the superior one to maintain conidial viability to the tune of 80.63% when compared to microcrystalline cellulose 6% and acacia gum, Arabic 5% which exhibited 30.67–51.50% viability. The investigation therefore concluded that L. saksenae is a promising bioagent for the management of root mealybugs with high speed of action unlike the other microbes. Biopellets formulated at 109 conidia/ml using talc + chitin + 6% CMC at 15% moisture content were found to retain its viability and virulence during a storage period of three months, under ambient conditions.

IMAC application of extracellular polymeric substances doped composite membranes for α-amylase immobilization and kinetic studies

Immobilized metal affinity chromatography (IMAC), extensively utilized technique in affinity chromatography, has proven to be highly effective in purifying α-amylase, a crucial enzyme in industrial applications. Here, Poly (2-hydroxyethyl methacrylate) (PHEMA) based composite membranes were fabricated using the radical copolymerization technique in a petri dish. Extracellular polymeric materials (EPS) as the metal binding agent. The attachment of Cu2+ ions to the membranes occurred through the utilization of EPSs (Cu2+-EPS). The membranes having Cu2+-EPS (Cu2+-EPS-PM) were subjected to various characterization techniques, including scanning electron microscopy (SEM), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), swelling tests, and surface area measurements. The investigation also encompassed the examination of various factors, including pH, initial α-amylase concentration, temperature, and ionic strength, that exert an influence on the adsorption of α-amylase from an aqueous medium. The Cu2+-EPS-PMs demonstrated maximum adsorption capacity with a value of 54.2 mg/g polymer (pH 5.0 sodium acetate buffer, temperature: 25°C, initial α-amylase concentration of 2 mg/mL). Additionally, it has been observed that the enzyme is able to be used in adsorption and desorption cycles on Cu2+-EPS-PM many times. The investigation also focused on examining the impact of pH, temperature, storage, and operational stability over the activities of both free and immobilized α-amylase. The outcomes showed that immobilization had positive effects on activity.

Zero-valent Iron Nanoparticles: Biogenic Synthesis and their Medical Applications; Existing Challenges and Future Prospects.

In the last decade, nanobiotechnology is emerging as a keen prudence area owing to its widespread applications in the medical field. In this context, zero-valent iron nanoparticles (nZVI) have garnered tremendous attention attributed to their cheap, non-toxic, excellent paramagnetic nature, extremely reactive surface, and dual oxidation state that makes them excellent antioxidants and free-radical scavengers. Facile biogenic synthesis, in which a biological source is used as a template for the synthesis of NPs, is presumably dominant among other physical and chemical synthetic procedures. The purpose of this review is to elucidate plant-mediated synthesis of nZVI, although they have been successfully fabricated by microbes and other biological entities (such as starch, chitosan, alginate, cashew nut shell, etc.) as well. The methodology of the study involved keyword searches of electronic databases, including ScienceDirect, NCBI, and Google Scholar (2008-2023). Search terms of the review included 'biogenic synthesis of nZVI', 'plant-mediated synthesis of nZVI', 'medical applications of nZVI', and 'Recent advancements and future prospects of nZVI'. Various articles were identified and reviewed for biogenic fabrication of stable nZVI with the vast majority of studies reporting positive findings. The resultant nanomaterial found great interest for biomedical purposes such as their use as biocompatible anticancer, antimicrobial, antioxidant, and albumin binding agents that have not been adequately accessed in previous studies. This review shows that there are potential cost savings applications to be made when using biogenic nZVI for medical purposes. However, the encountering challenges concluded later, along with the prospects for sustainable future development.

Influence of biochar in the calcite precipitation of sandy soil using sporosarcina ureae

The microbially induced calcium carbonate precipitation (MICP) technology is an emerging novel and sustainable technique for soil stabilization and remediation. MICP, a microorganism-mediated biomineralization process, has attracted interest for its potential to enhance soil characteristics. The inclusion of biochar, a carbon-rich substance formed by biomass pyrolysis, adds another degree of intricacy to this process. The study highlights the impact of the combination of biochar and MICP together, using a bacterium, Sporosarcina ureae, on soil improvement. This blend of MICP and biochar improved the soil in terms of its geotechnical properties and also enabled the sequestering of carbon safely. It was observed that addition of 4% biochar significantly increased the soil's shear strength parameters (c and φ) as well as its stiffness after 21 treatment cycles. This improvement was because the calcium carbonate precipitate, which acts as a crucial binding agent, increased significantly due to microbial action in the soil-biochar mixture compared to the pure soil sample. The excess carbonate precipitation on account of biochar addition was verified through SEM-EDAX analysis where the images showed noteworthy carbonate precipitation on the surface of particles and increment in the calcium mass at the same treatment cycles when compared with untreated sand. The collaboration between MICP and biochar effectively increased the carbon sequestration within the sand sample. It was observed that at 21 cycles of treatment, the carbon storage within the sand sample increased by almost 3 times at 4% biochar compared to sand without any biochar. The statistical analysis further affirmed that strength depends on both biochar and the number of treatment cycles, whereas carbon sequestration potential is primarily influenced by the biochar content alone. This strategy, as a sustainable and environmentally friendly approach, has the potential to reform soil improvement practices and contribute to both soil strength enhancement and climate change mitigation, supporting the maintenance of ecological balance.

Biochar addition promotes soil organic carbon sequestration dominantly contributed by macro-aggregates in agricultural ecosystems of China

Soil aggregates play pivotal roles in soil organic carbon (SOC) preservation and climate change. Biochar has been widely applied in agricultural ecosystems to improve soil physicochemical properties. However, the underlying mechanisms of SOC sequestration by soil aggregation with biochar addition are not well understood at a large scale. Here, we conducted a meta-analysis of 2335 pairwise data from 45 studies to explore how soil aggregation sequestrated SOC after biochar addition in agricultural ecosystems of China. Biochar addition markedly enhanced the proportions of macro-aggregates and aggregate stability, and the production of organic binding agents positively facilitated the formation of macro-aggregates and aggregate stability. Soil aggregate-associated organic carbon (OC) indicated a significantly increasement by biochar addition, which was attributed to direct and indirect inputs of OC from biochar and organic residues, respectively. Biochar stimulated SOC sequestration dominantly contributed by macro-aggregates, and it could be interpreted by a greater improvement in proportions and OC protection of macro-aggregates. Furthermore, the SOC sequestration of soil aggregation with biochar addition was regulated by climate conditions (mean annual temperature and precipitation), biochar attributes (biochar C/N ratio and pH), experimental practices (biochar addition level and duration), and agronomic managements (land type, cropping intensity, fertilization condition, and crop type). Collectively, our synthetic analysis emphasized that biochar promoted the SOC sequestration by improving soil aggregation in agricultural ecosystems of China.

Facile preparation of superhydrophilic and superoleophobic sand for efficient oil-water separation

Superhydrophilic-superoleophobic materials possess unique advantages in oil-water separation due to their water permeability and oil repellency. In this study, superhydrophilic-superoleophobic sand (CQS) was prepared using a facile and rapid mixing-drying coating method. Quartz sand was modified with polydopamine (PDA) as the hydrophilic binding agent, fluorosurfactant (FS-50) as the oleophobic agent, and CaCO3/SiO2 to construct hierarchical surface roughness. The CQS exhibited a water contact angle of 0° and an oil contact angle of 154°, which resulted in a slightly increased water adhesion capacity and a significantly reduced oil adhesion capacity. Owing to its selective wettability towards oil and water, the CQS was capable of directly separating immiscible oil-water mixtures and oil-in-water emulsions with high separation efficiency (99.5 % and 98.5 %, respectively), driven by gravity. Moreover, immiscible oil/oil mixtures could be effectively separated by the CQS as well, expanding its application to separating immiscible organic liquids with different polarities. Additionally, the CQS demonstrated good reusability and superior stability against acid, alkali, salt, hot water and mechanical abrasion. These properties ensured its applicability in complex environments. The CQS, with exceptional separation capability and stability, holds promising prospects for future applications in related separation field.

Synthesis and characterization of cellulose nanofibers from waste paper and their utilization in wood adhesion

AbstractNanocellulose extraction from lignocellulosic materials is a highly chemical and energy‐intensive process as it requires the removal of lignin and hemicellulose. Writing paper is one of the processed materials that could be used as a raw material for the extraction of nanocellulose. In this work, cellulose nanofibers (CNFs) were synthesized from paper waste using TEMPO‐oxidation followed by high‐shear microfluidization. Scanning electron microscopy and atomic force microscopy confirmed the diameter of fibers in the nano‐range and a consistent zeta potential confirmed the stability of CNF suspension in water over a long time frame. Characterization of the CNFs using Fourier transform infrared spectroscopy indicated the presence of a carbonyl group due to the oxidation process. Thermogravimetric analysis and x‐ray diffraction revealed lower thermal stability and reduction in the crystallinity index of CNFs, as compared to pulp fibers. The obtained CNFs were used successfully as the sole binding agent in the preparation of fiberboards and also utilized as a reinforcing agent for polyvinyl acetate (PVAc) adhesive in the preparation of laminated veneer lumber (LVLs). The addition of CNFs in PVAc improved the glue shear strength indicating superior bonding characteristics and also increased the water resistance of the LVLs.Highlights This work focused on cellulose nanofibers (CNFs) extraction from waste paper Obtained CNFs form stable water suspension and have a diameter of less than 15 nm Thermal stability and crystallinity index reduced after conversion to nanofibers CNFs form a complex network and act as a sole binder to make fiberboards CNFs were utilized as a reinforcing agent for PVAc in preparation of LVLs

Peptidic Compound as DNA Binding Agent: In Silico Fragment-based Design, Machine Learning, Molecular Modeling, Synthesis, and DNA Binding Evaluation.

Cancer remains a global burden, with increasing mortality rates. Current cancer treatments involve controlling the transcription of malignant DNA genes, either directly or indirectly. DNA exhibits various structural forms, including the G-quadruplex (G4), a secondary structure in guanine-rich regions. G4 plays a crucial role in cellular processes by regulating gene expression and telomerase function. Researchers have recently identified G4-stabilizing binding agents as promising anti-cancer compounds. Additionally, peptides have emerged as effective anticancer pharmaceuticals due to their ability to form multiple hydrogen bonds, electrostatic interactions, and van der Waals forces. These properties enable peptides to bind to specific areas of DNA chains selectively. However, despite these advancements, designing G4-binding peptides remains challenging due to a lack of comprehensive information. In our present study, we employed an in silico fragment-based approach to design G4- binding peptides. This innovative method combines machine learning classification, molecular docking, and dynamics simulation. AutoDock Vina and Gromacs performed molecular docking and MD simulation, respectively. The machine learning algorithm was implemented by Scikit-learn. Peptide synthesis was performed using the SPPS method. The DNA binding affinity was measured by applying spectrophotometric titration. As a result of this approach, we identified a high-scoring peptide (p10; sequence: YWRWR). The association constant (Ka) between p10 and the ctDNA double helix chain was 4.45 × 105 M-1. Molecular modeling studies revealed that p10 could form a stable complex with the G4 surface. The obtained Ka value of 4.45 × 105 M-1 indicates favorable interactions. Our findings highlight the role of machine learning and molecular modeling approaches in designing new G4-binding peptides. Further research in this field could lead to targeted treatments that exploit the unique properties of G4 structures.

Predicting the precipitated calcium carbonate and unconfined compressive strength of bio-mediated sands through robust hybrid optimization algorithms

Biological soil stabilization through Microbial-Induced Calcite Precipitation (MICP) demonstrates the potential to be an eco-friendly and sustainable approach to enhance the strength properties of cohesionless granular soils. In this study, based on a comprehensive set of experimental data compiled from literature, the amounts of Precipitated Calcium Carbonate (PCC) and Unconfined Compressive Strength (UCS) of MICP-treated sands are estimated through hybridizing Artificial Neural Network (ANN) and Adaptive Neuro-Fuzzy Inference System (ANFIS) models with metaheuristic algorithms. During the data extraction procedure, a total of 70 and 115 datasets are used to predict the PCC and UCS, respectively. Accordingly, five key features of urea concentration (Mu), calcium chloride (Mcc), optical density of bacterial suspension (OD600), pH and total volume of injection per volume of sample (Vt), are first selected as the inputs of the hybrid models to estimate the amount of calcium carbonate precipitation within the porous structure of MICP-treated granular medium. The experimental data on the PCC along with the basic characteristics of the host sand including mean grain size (D50), uniformity coefficient (Cu) and void ratio (e), are then adopted as the inputs of the numerical models in the second phase whilst the UCS of bio-mediated sands is set as the output. The performance and accuracy of implemented models are rigorously assessed through analyzing various statistical indices and performance criteria. According to the numerical analysis, the ANN-PSO and ANFIS-PSO hybrid models show the best performance in predicting PCC and UCS of bio-mediated sands, respectively. Using the well-established Gene Expression Programming (GEP) algorithm, practical correlations are developed to predict the PCC and UCS values of MICP-mediated soils based on the characteristics of biological binding agents as well as the host material. The Machine Learning (ML)-based models presented in this study provide engineers with a fruitful framework for the rough and preliminary estimation of the amount of calcite precipitation as well as the UCS of bio-mediated sands in the field prior to stabilization and performing complementary tests.

Synthesis, antioxidant, DNA interaction, electrochemical, and spectroscopic properties of chromene-based Schiff bases: Experimental and theoretical approach

Two chromene-based Schiff base ligands were synthesized by the condensation of 5-hydroxy-2,2-dimethyl-2H-chromene-6-carbaldehyde with 2-aminodiphenylamine and 1,8-diaminonaphthalene: (E)-2,2-dimethyl-6-(((2-(phenylamino)phenyl)imino)methyl)-2H-chromen-5-ol (2) and (E)-6-(((8-aminonaphthalen-1-yl)imino)methyl)-2,2-dimethyl-2H-chromen-5-ol (3). The compounds were characterized by Fourier transform infrared (FT-IR), absorption (UV-Vis) and nuclear magnetic resonance (NMR) spectroscopies, in addition to mass spectrometry (MS) analyses; the results of which were rationalized using density functional theoretical (DFT) calculations. Antioxidant activity of the Schiff bases was determined using the DPPH free radical scavenging and CUPric Ion Reducing Antioxidant Capacity (CUPRAC) assays. Compound 3 (IC50 = 14 μM) showed greater free radical scavenging ability than compound 2 (35 μM), and was also more effective than ascorbic acid and Trolox. DFT studies indicate that the HAT mechanism is favored over SET for DPPH scavenging. Both compounds exhibited stronger Cu(II) ion reducing capacity than Trolox and ascorbic acid with Trolox equivalent activity coefficient (TEAC) values of 2.0 and 1.6 for compounds 2 and 3, respectively. Furthermore, DNA cleavage and binding properties were determined using gel electrophoresis, UV-Vis spectroscopy, and cyclic voltammetry. The compounds showed copper-mediated cleavage of plasmid DNA and strong binding to DNA, with binding constants on the order of 104 M−1. The strength of DNA binding was verified by molecular docking analysis using two DNA models: a B-DNA dodecamer (-8.4 to -9.3 kcal/mol) and a hexamer (-7.8 to -7.9 kcal/mol). Overall, the chromene Schiff bases show promise as antioxidant and DNA binding agents.

Soil colloids as binding agents in the formation of soil microaggregates in wet-dry cycles: A case study for arable Luvisols under different management

In the hierarchical model of soil aggregates, small soil microaggregates (small SMA; <20 μm) are often considered to be fundamental building units at the micron scale. Below which, soil colloids (<1 µm) have recently been proposed as binding agents of (micro)aggregates. However, the way in which soil colloids contribute to the formation and stability of soil micro- and macroaggregates remains largely unknown. For clarification, we evaluated potential impacts of the colloidal content, particularly the <450 nm colloids, on the aggregation of small SMA. Free water stable small SMA and <450 nm colloids were isolated from Ap-horizons of Stagnic Luvisols under different management (cropped and bare fallow). The size-resolved elemental composition of the <450 nm colloids was analyzed by asymmetric flow field-flow fractionation in combination with an inductively coupled plasma mass spectrometer and an organic carbon detector. To vary the colloidal content in small SMA, (1) suspensions containing different amounts of <450 nm colloids were added in small SMA, or (2) <1 µm colloids were removed from small SMA by centrifugation. In the maximum colloidal addition treatment, the mass ratios of added colloids to small SMA were 3.0 and 5.1 wt% for the cropped and bare fallow soil samples, respectively. Aggregation of small SMA with different colloidal amounts was performed in three successive wet-dry cycles. Afterwards, the size distribution of the resulting aggregates was measured by laser diffraction. Our results indicated that, in wet-dry cycles, colloids were important binding agents for the formation of SMA. Their presence, especially those <450 nm, was likely to support the formation of solid bridges during drying at particle contacts of 1–10 µm small SMA, favoring hereby SMA build-up in a relatively small size range of 1–40 µm. In contrast, the absence of <1 μm colloids in small SMA led to a preferential generation of relatively large aggregates in wet-dry cycles, i.e., typically with sizes >40 μm up to 1700 μm in maximum. Our study on aggregation in wet-dry cycles revealed that the colloidal content has a controlling effect on the size distribution of resulting aggregates by acting as a binding agent and provides hereby new insights into the evolvement of aggregate hierarchy in soils.

The effect of probe density coverage on the detection of oenological tannins in Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) experiments.

Polyphenols are a group of compounds found in grapes, musts, and wines. Their levels are crucial for grape ripening, proper must fermentation, and final wine characteristics. Standard chemical analysis is commonly used to detect these compounds, but it is costly, time-consuming, and requires specialized laboratories and operators. To address this, this study explores a functionalized acoustic sensor for detecting oenological polyphenols. The method involves utilizing a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect the target analyte by using a gelatin-based probe layer. The sensor is functionalized by optimizing the probe coverage density to maximize its performance. This is achieved by using 12-mercaptododecanoic acid (12-MCA) to immobilize the probe onto the gold sensor surface, and dithiothreitol (DTT) as a reducing and competitive binding agent. The concentration of 12-MCA and DTT in the solutions is varied to control the probe density. QCM-D measurements demonstrates that the probe density can be effectively adjusted using this approach, ranging from 0.2×1013 molecules cm-2 to 2×1013 molecules cm-2 . This study also investigates the interaction between the probe and tannins, confirming the sensor ability to detect them. Interestingly, the lower probe coverage achieves higher detection signals when normalized to probe immobilization signals. Moreover, significant changes in mechanical properties of the functionalization layer are observed after the interaction with samples. The combination of QCM-D with gelatin functionalization holds great promise for future applications in the wine industry. It offers real-time monitoring capabilities, requires minimal sample preparation, and provides high sensitivity for quality control purposes. This article is protected by copyright. All rights reserved.

A comprehensive guide to Andopanaha - A modified Swedana procedure that can be utilized in Panchakarma in various forms

Introduction: The Upanaha Sweda and Anda Pinda Sweda have wide levels of application in Ayurveda practice. Upanaha Sweda is mentioned by Acharya Charaka in Niragni Sweda type. Whereas, other classics like Susruta Samhita and Astanga Hrudaya mention it in the Sagni Sweda variety. So, combining both the views of Acharyas, we can interpret that there are 2 types of Upanaha i.e., Sagni Upanaha &amp; Niragni Upanaha. Anda Pinda Sweda is a type of Snigdha Sankara Sweda. These two classically mentioned therapies are less utilized in many institutions due to various reasons. Here in this study, we are trying the sort out the practical difficulties and find the possible modifications of these classical procedures for their utilization in the modern era. Methodology: With the main intention of developing a Snigdha Swedana procedure having both the benefits of Anda Pinda Sweda and Upanaha Sweda, the Andopanaha is designed. Here in this new method, the Dhanya Dravya is replaced by the (Kukkuta Anda) egg white, which is also a good binding agent. Observations: Compared to the Anda Pinda Sweda procedure the contact of Egg proteins with the skin is more in the Andopanaha procedure. Which helps is more analgesic effects and anti-inflammatory effects of the egg proteins. Discussion: The paper is prepared by authors with the intention that procedures and standards of care must be updated with modern tools and technologies without violating the basic principles of Ayurveda. If the old procedures need any changes according to the modern era they must be followed for their propagation in the public.