Bila Tserkva Research Articles

Features of development of winter wheat plants in the autumn-winter vegetation period as affected by pre-sowing seed treatment

Purpose. To identify the peculiarities of growth and development of winter wheat plants in the autumn-winter vegetation period as affected by pre-sowing seed treatment in the conditions of the Right-Bank Forest-Steppe of Ukraine.
 Methods. The experiments were carried out at the Agronomic Research Station of the National University of Life and Environmental Sciences of Ukraine (Pshenychne, Bila Tserkva district, Kyiv region) in the years 2019–2021: inoculant Binok Grain, complex micro fertilizer Harvest Start, organic biostimulant containing enzymes Rhizomax and bacterial formulations Trichodermin and Planoryz at the application rates recommended by their manufacturers. The background (control) for the study was a treatment where seedbed preparation included the application of nitroammophos N32P32K32 together with a complex granular fertilizer Actibion. The cultivation technology in the experiment is generally accepted for the research area.
 Results. In the control treatment, on average for the years of research, the field germination of bread winter wheat seeds was 82.7% with a plant density of 414 per 1 m2. The use of protective and stimulating formulations for seed treatment increased these indicators compared to the control by 1.9–4.8% and 9–26 plants/m2, respectively. The highest sugar content in the tillering node (14.2%) and, accordingly, winter hardiness of plants (96.8%) was obtained in the treatment with Binok Grain + Harvest Start. In other seed treatments, these indicators ranged between 12.6 and 14.0% and 93.4–96.0%, while in the control between 12.4% and 82.7%, respectively. In the control treatment, the incidence of Septoria in autumn was 1.6%, powdery mildew 5.6%, and Fusarium root rot 2.9%, while in the case of using biological formulations the values were 0.1–0.9%, 0.1–3.0 and 0.0–1.4%, respectively. Complete control of these diseases in sowings (plant damage was not detected) is provided by the combined use of Binok Grain + Harvest Start. Most likely, this is because Binok Grain contains antagonists of pathogens, phytohormones, antibiotics, vitamins, amino acids, and growth regulators. Harvest Start, in addition, to trace elements, contains auxins, amino acids, and B vitamins.
 Conclusions. The use of all studied protective and stimulating formulations for the winter wheat seed treatment ensured a significant increase in the field germination of seeds, plant density, sugar content in the tillering node, winter hardiness, and limit the development of dangerous pathogens in crops. Consequently, these biological formulations may be recommended for implementation in the current cultivation technologies practiced in the Right-Bank Forest-Steppe of Ukraine.

Analysis of varietal diversity, decorativeness and peculiarities of growth and development of cultivars Dahlia cultorum Thorsrud & Reisaeter in the collection of the biostationary of Bila Tserkva NAU

Aim. In order to identify promising cultivars of Dahlia cultorum for use in landscaping in Kyiv region, the analysis of the system of qualitative and quantitative indicators of cultivars of dahlias, the peculiarities of their growth and development in the absence of irrigation is conducted. Methods. To analyze the diversity of Dahlia cultorum cultivars at the biostationary of Bila Tserkva NAU, the classification developed by the American Society of Dahlia (ADS classification) was used. The cultivar assortment of dahlias was studied according to the descriptions of the catalog of the company “SontseSad”, which transferred 108 cultivars of Dahlia cultorum to Bila Tserkva NAU biostationary in 2014. Phenological rhythms of growth and development of dahlias were studied according to the methods of B. K. Shybniev, H. D. Yurkevych, and D. S. Holod. The increment of dahlias was measured during the period of active growth, the size of the flower — during the period of its full opening with the help of a measuring ruler on 5 specimens of each cultivar every decade. Results. Based on the analysis of the varietal composition of Dahlia cultorum collection of dahlia cultivars of Bila Tserkva NAU biostationary, it was found that the largest number of 36% belongs to the cultivar group of decorative dahlias, 12% — to cactus, 7% — to spherical and 1% to pompoms and anemones. The mixed cultivar of dahlias is 43.4%, among them the most numerous group of dahlias with dissected leaves “Deer antlers” — 48%, “Karma” — 19%, pot — 12%, French collection — 21%. According to the color spectrum, the collection is dominated by dahlias of red color — 29%, pink — 19%, yellow and orange — 16% each, violet — 12%, white color — 6%. Two-color cultivars amount to only 2%. Observations show that in May–June, due to the long and dry period and the lack of artificial irrigation, all cultivars of dahlias at Bila Tserkva NAU biostationary were marked by very slow growth. As of July 15, the height of dahlia bushes averaged 30–50 cm, which was significantly less than the size presented in the catalog of the magazine “SontseSad”. Conclusions. The collection of dahlias at Bila Tserkva NAU biostationary includes 108 cultivars belonging to seven main cultivar groups, including 11 cultivars from the new French collection. Early in the first decade of July, the cultivars ‘Islande’, ‘Maki’, ‘Sir Alf Ramsay’, ‘Mingus Alex’, ‘Glorie van Heemstede’ begin to bloom. Latest of all, in August, cultivars ‘Babylon Rose’, ‘Amanda’, ‘Aitara Bronwyn’, ’Karma Gold’, ‘Gallery Rembrandt’, ‘Lilac Time’, and ‘Star Spectacle’ began flowering. It is established that the period from the appearance of the bud to the opening of the flower in dahlia cultivars lasts 25-30 days. The first buds appeared in early July, but their peduncles were very short and such flowers were not suitable for cutting. In fact, the blooming central flower itself was 5-10 cm lower than the second-order buds around it, and therefore had a limited view from the inflorescence, which led to a loss of decorativeness in the cultivars ‘Romance’, ‘Candlelight’, and “Karma Bon Bini”. The duration of flowering of one dahlia flower in different cultivars ranged from 5 to 17 days. The longest period of blooming of one flower is 14-17 days in ‘Autumn Fairy’, ‘Nagano’, ‘Idylle’, ‘Babylon Brons’, ‘Duet’ cultivars. The shortest blooming period of one flower is 5-7 days for ‘Ellen Huston’, ‘Champs Elysees’, ‘ThykiYori No Shisha’, ‘Aspen’ cultivars. Due to the high air temperature and the long rainless period, the peak of flowering of dahlias in the conditions of Bila Tserkva NAU biostationary was at the beginning of September.

Monitoring of productive and biochemical parameters of young Clarias gariepinus during feeding of Skretting and Roycher AQUA feeds in experimental conditions

The article presents the results of monitoring the productive and biochemical indicators of the growth rate of young clary catfish using dry extruded complete feed for fish, which have proven to be best adapted for use at all stages of growing clary catfish, namely: Skretting and Roycher AQUA in experimental conditions. The research was conducted in the educational and experimental laboratories of Bila Tserkva National Agrarian University from 2019 to 2021. For the experiment, we used the young of the African marble clary catfish (Clarias gariepinus), purchased from one of the fish farms in the Odesa region. Clary catfish fry was kept in four rectangular plastic containers for 90 liters of freshwater. The tanks were two-thirds full (60 liters). The water temperature was maintained at 26 °C. For the experiment, 120 fries were used, which were kept in four plastic tanks by the method of analogs of 30 specimens each. Given that the fry differed in weight (for experiment 1 we selected fry with higher weight, and for experiment 2 – with lower weight), they were divided on the principle of analogs into two experimental and two control groups. Based on the results of monitoring in Experiment 1, fish observations, and mathematical calculation, we found that the feed ratio when fed Skretting and Roycher AQUA starter feed is 0.74 and 0.99 %, respectively. Based on the results of monitoring, fish observations, and mathematical calculation, it was found that the feed ratio in experiment 2 when feeding starter feed Skretting and Roycher AQUA is 0.75 and 1.54 %, respectively. The high feed rate for the use of Roycher AQUA can be explained by overuse due to the grinding of the granules. Growing aquaculture facilities in an artificially formed system – production, allows you to get environmentally friendly fish products all year round. This is a very important criterion in modern environmental conditions.

The economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the rations of highlyproductive cows of the Ukrain-ian Red-Spotted Dairy breed

The article deals with the results of research on the economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the feeding rations of highlyproductive cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation. The research was performed on five groups (one control and four experimental) of analogous cows in the conditions of ALC “Terezyne” Bila Tserkva district of Kyiv region. The control was the optimal dose of mixed ligand complexes of Zinc, Manganese and Cobalt, which was previously established with a concentration of 1 kg of dry matter (DM) of the feed mixture (FM), mg: Zinc – 60.8; Manganese – 60.8 and Cobalt – 0.78. For the 2nd experimental group, the concentration of these trace elements was increased by 10 %, and in the 3rd, 4th and 5th experimental groups – on the contrary, the concentration of Zinc, Manganese and Cobalt in 1 kg of DM of feed mixture was reduced by 10 %, 20 and 30 %, respectively, compared with the control. The best results on milk yield of natural and basic fat content were got from cows of the 4th experimental group, where the concentration of Zinc, Manganese and Cobalt due to their mixed ligand complexes in 1 kg of DM of feed mixture was, mg: Zinc – 48.6; Manganese – 48.6; Cobalt – 0.62. The cost of products got from experimental cows ranged from UAH 12702.3 up to UAH 13783.7 and it was the highest in the 4th experimental group. It was set up that for cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation the most economically advantageous was the dose of mixed ligand complexes, in which the concentration of Zinc and Manganese was 48.6 mg, and Cobalt – 0.62 mg/kg DM, which allowed to obtain a profit of 14.37 % more than in the control group. Doses of mixed ligand complexes with a concentration of Zinc and Manganese – 66.9 mg; 54.7 and 42.6 mg, and Cobalt – 0.86 mg; 0.7 and 0.55 mg/kg CF gave a much lower economic effect. Additional profit in the 2nd experimental group amounted to UAH 66.8 or 1.34 %; in the 3rd – UAH 95.6 or 1.92 % and in the 5th – UAH 561.6 or 11.28 %, compared with the control group.

The assessment of the ecological condition of the Ros River within the Belotserkovsky district

The Ros River is polluted with heavy metals, petroleumproducts and organic matter due to the activities of variousindustries, including agriculture (washing away pesticides and fertilizers), food, light and petrochemical industries, etc.Intake of drinking water in Bila Tserkva district is carried outfrom the river Ros, so the aim of the work was to systematizethe results of research over the past 10 years and identify thedynamics of changes in the ecological state of the river.Object of research: Ros River within Bila Tserkva district of Kyiv region.Subject of research: negative environmental factors,water quality indicators. The study of the state of the question was carried out by methods of analysis and synthesis.Water quality assessment was conducted in the Ros Riveron a monthly basis. Initial data (monthly results) are averaged by seasons: spring (March-May, 2010-2020), summer(June-August, 2010-2020), autumn (September-November,2010-2020), winter (December of the previous year - January, February of the current year, 2010/2011-2020/2021).Among the studied indicators there are ammonium salt, nitrates, nitrites, chemical oxygen demand (COD), biochemicaloxygen demand (BOD5), phosphates, total iron, manganese.Seasonal concentrations throughout the study period for ammonium salt ranged from 0.11 to 2.17 mg/dm3, nitrites – from0.01 to 0.82 mg/dm3, nitrates did not exceed 40.0 mg/dm3,COD– in the range from 15.9 to 61.1 mg/dm3, BOD5– in therange from 1.5 to 8.3 mg/dm3, phosphates – from 0.05 to 0.49mg/dm3. Excess concentration of total iron and manganesewere observed in 59% and 29% of cases, respectively. Thatis, there is a tendency to deterioration water quality by somecomponents; the main reason for this is anthropogenic activity, namely the inconsistency of water treatment equipmentof economic entities with modern environmental standardsand the presence of unidentified sources of discharges intothe Ros River basin. Key words: Rosriver, hydrochemical indicators, ecological condition, seasonal values, MPC.

Productivity and fertilizer value of white mustable mustard on green fertilizer depending on preparators, main processing systems and processing

Three-year (2019–2021) studies in a stationary feld grain-planted fve-feld crop rotation on typical low-humus medium-loam chernozem of the experimental feld of the Bila Tserkva NAU indicate a higher productivity of white mustard for green fertilizer when sown after winter wheat than spring barley. On no-till and systematic shallow tillage in the rotation, it signifcantly reduces the yield and dry weight of the roots. After the spring precursor sideratu mass is signifcantly higher after the winter one – not signifcantly lower on differentiated than the shallow tillage in the rotation. On cheisel and especially permanent shallow tillage in the rotation the effectiveness of fertilizers with increasing rates of their application decreases. With increasing fertilizer rates, the rate of growth of above-ground mass is higher than that of the root system. They tend to be lower when cabbage plants are sown after spring barley than winter wheat. Dry mass of root residues of white mustard after winter crops is practically at the same level on ploughed and ploughed-free tillage, and after spring – signifcantly higher on differentiated tillage than on the control. The dry mass of root residues of a crop plant in the arable soil layer was 3.03 and 2.59 t/ha, respectively, under the above precursors. The gain of humus content in arable layer by sowing the investigated crop after the winter wheat and after rotation without plowing, differentiated and disk tillage was respectively 532, 503, 525 and 474 kg/ha and after sowing the investigated crop after the spring barley – 453, 403, 488 and 393 kg/ha. At the date of sowing of cabbage plants after winter wheat, the reserves of available moisture in the upper layer of the soil under tillage are signifcantly lower, and under differentiated – signifcantly higher than in the control. When sowing white mustard after spring barley, this fgure is the lowest for shelf cultivation, and the highest – for shelf-shelfless cultivation in crop rotation. Key words: soil, crop rotation, white mustard, treatment, fertilizers, predecessors, yield, root residues.

The determination of toxic level of selenium preparations

Poultry farming has a significant need for balanced nutrients to ensure high growth rates. Traditionally used selenium drugs do not fully provide protection against the effects of stressors due to the narrow range of non-toxic doses, difficult assimilation of inorganic drugs, the difficulty of regulating the biological effect of the use of organic forms of compounds. The new selenium nanosized preparations have a higher surface-to-volume ratio than traditional preparations, have a wider range of non-toxic doses, and can otherwise affect metabolic processes according to the characteristics of the nanoparticles used. To establish the appropriateness of their use in poultry feeding, itwas studied the toxicity of selenium and probiotic preparations byintragastric administration to laboratory animals. In collaboration with the Institute of D.K. Zabolotny Microbiology and Virology Institute of National Academy of Sciences of Ukraine at the Research Institute of Ecology and Biotechnology of Bila Tserkva National Agrarian University there was developed a technology of "green" production of a multifunctional antioxidant - nanopopulation "Nano". Indicators of acute and acute toxicity of sodium selenite with probiotic and bionanoselen were studied in white mice and rats, to which the studied feed additives were administered intragastrically for 14 and 30 days in different doses. The next day after administration, rats were decapitated under light ether anesthesia, blood samples were taken, hematological tests were performed according to generally accepted methods and organ mass ratios were determined. The study allows us to conclude about the low-toxic effect of selenium preparations on experimental animals, their significant effect on the growth of certain hematological parameters of the blood and the absence of significant changes in the weights of internal organs. Significant differences in the mass of internal organs: liver, lungs, heart and kidneys were not detected, indicating the absence of toxic effects of nonoselen in different doses on these organs. Key words: nanopreparations, selenium, toxicity, rats, mice, blood, internal organs.

Dynamics of heart rate variability and the state of endogenous in toxication under different anesthesia regimens in dogs with visceral and somatic types of pain reaction

Dogs are naturally characterized by a high level ofinjuries, which can reach up to 50 % of the total surgicalpathology. At the same time, abdominal pathology issignificantly common in this species of animals andsurgical treatment of the sepathologies is accompanied bya significant somatic and visceral pain response. The workwas performed at the Department of Surgery and Diseasesof Small Pets of Bila Tserkva National Agrarian Universityin the period 2015-2020. The material for the study wereclinically healthy and sick dogs admitted to the clinic ofdiseases of small animals of the university. Forming theclinical-experimental base for research, nosological formsof pathology and frequency of its occurrence were taken into account, attention was paid to the peculiarities of surgicalintervention depending on the species of animals, type andintensity of pain response and correctness of anesthesia.Visceral type of pain reaction was studied in dogs aged2 to 10 years, who underwent abdominal surgery. Somaticpain reaction accompanies bone fractures and their surgicaltreatment. Included in the study of dogs with fractures of thefemur or humerus aged from 1 to 10 years.High levels of endotoxicosis are characteristic of thepathochemical phase of abdominal surgical pathology in dogs,with the concentration in the blood of malonic dialdehyde(14.8 ± 0.55 μmol / l) and molecules of average weight isrespectively 1.6 and twice higher than normal. However,the lowest level was found under balanced acepromazineketamine-propofol anesthesia, with no probable changes inmolecular weight peptides and a slight increase in the level ofmalonic dialdehyde only 1.1 times (p <0,01).Acepromazine-butorphanol-propofol-ketamineanesthesia for somatic pain in dogs provides completeanalgesia with controlled and rapid recovery fromanesthesia without established probable changes during theoperation of spectral indicators of heart rate variability. Insomatic pain in dogs, regardless of the anesthesia regimen,the preoperative level of endotoxemia is preserved, andaccording to the spectral analysis of HRV and its sympathoparasympathetic balance index, 30 % of surgically ill dogsshow normotonia – 0.85–1.15, 27 – sympathicotonia (LF/HF>1.15), in 43 % – parasimaticotonia (LF/HF<0.85),which is the key criterion for selecting the appropriatescheme of anesthesia. Key words: heart rate variability, endogenousintoxication, anesthesia, dogs, types of pain reaction, visceralinnervation, somatic innervation.

Grain productivity hybrids of corn depending on the use of complex mineral fertilizers

The results of studying the effect of complex mineral fertilizers on the productivity of corn hybrids of different ripeness groups in the conditions of the Right-Bank Forest-Steppe are presented. The studies were carried out in 2019–2021 in the conditions of the experimental feld of the Scientifc and Production Center of the Bila Tserkva National Agrarian University. The experiment studied 9 early, mid-early and mid-season corn hybrids (FAO 170–350) and 4 levels of fertilizers: no fertilizers, N60P60K60, N60P60K60 + Plantonit Frumentum (1 l/ha), N60P60K60 + Plantonit Grain (1 l/ha). It was found that all the studied hybrids had high parameters of the grain mass from the kernel and the mass of 1000 grains when using N60P60K60 + Plantonit Frumentum. There was no signifcant difference in the elements of the yield structure when using N60P60K60 + Plantonit Frumentum and N60P60K60 + Plantonit Grain. The maximum grain yield was provided by mid-season hybrids Carifols and LG30352 on the variant N60P60K60 + Plantonit Frumentum – 8.21 and 7.84 t/ha. When N60P60K60 + Plantonit Grain was applied, the yield was 8.16 and 7.81 t/ha. The grain yield of the hybrids of the early maturing group was 5.82 t/ha, the medium-early – 6.71 t/ha, and the mid-season – 7.35 t/ha. Among the hybrids of the early-maturing group, the most productive was LG30189 (4.75–6.63 t/ha), in the medium-early group – P8409 (5.29–7.61 t/ha), in the mid-season group – Carifols (6.04–8.21 t/ha). Due to the use of complex mineral fertilizers Plantonit Frumentum and Plantonit Grain, the productivity of the crop increased by an average of 6.0 and 5.4 % compared to the application of only N60P60K60. When N60P60K60 + Plantonit Grain was applied, early-maturing hybrids increased grain yield by 1.76 t/ha, medium-early by 2.11 t/ha, mid-season by 1.96 t/ha compared to the variant without fertilizers. At the same time, the greatest increase yield was observed in medium-early and mid-season hybrids (1.48–2.32 t/ha). The effect of mineral fertilizers on the moisture content of corn grain has not been established, and this indicator depended on the biological characteristics of the studied hybrids. Key words: corn, grain, hybrid, mineral fertilizers, productivity, foliar feeding.

VOLODYMYR FRANKOVYCH STAKHOVSKYI – RECOGNIZED AUTHORITY IN ANIMAL REPRODUCTION

VOLODYMYR FRANKOVYCH STAKHOVSKYI – RECOGNIZED AUTHORITY IN ANIMAL REPRODUCTION

Evaluation of honey as a dessert in hotel and restaurant complexes

The article presents the results of research of honey as a dessert in hotel and restaurant complexes of Bila Tserkva region. The analysis of researched samples of honey with the use of laboratory researches which were carried out in the conditions of scientific laboratory of chair of safety and quality of foodstuff, raw materials and technological processes of Bila Tserkva national agrarian university is carried out. The research was conducted in accordance with the national standard of Ukraine DSTU 4497. according to generally accepted methods. Color, taste, aroma, consistency, crystallization, signs of fermentation were determined organoleptically using the sensory method of analysis of honey. It was found that in all samples the color of honey was from light yellow to yellow, with a pleasant taste and aroma, without signs of fermentation and the content of mechanical and other impurities. The diastasis number of honey samples used by hotel and restaurant complexes is 14, 10 and 8 units, respectively. Goethe, which indicates the heating of part of the honey. Thus, the results of research have shown that honey, which is used in hotel and restaurant complexes of Bila Tserkva region as a dessert in terms of organoleptic indicators, meets the requirements of the national state standard.

Вплив використання засобів охолодження повітря у легкозбірних приміщеннях на показники поведінки та комфорту корів за високих температур навколишнього середовища

The aim of this work was to study the influence of the use of air cooling means in easily assembled premises on the behavior and comfort of cows during periods of dangerous value of the temperature-humidity index (THI). The research was conducted during July 2020 in the central part of Ukraine (Kyiv region, Bila Tserkva district) during a long high-temperature load. The value of the daily ambient air temperature ranged from +19 to +34°С, and the relative humidity was 46–78%. Two farms with loose cows and different animal comfort options were selected for the study. The first option is to be loose in an easy-to-assemble room with fans installed above the aft table to cool the air. Fan hours from 12:00 to 17:00. The second option is loose keeping in an easy-to-assemble room on a deep, long-lasting straw litter. It was found that the use of ventilation systems allowed to reduce by 5.3°C the average daily values of air temperature compared to keeping on deep litter without ventilation systems. The values equivalent temperature index (ETI) and the index of equivalent temperature for cattle (ETIC), which indicate the level of sensitivity of the body during prolonged high temperatures, were lower by 1.63°C, and ETIC by 1.11 compared to keeping in deep litter without systems ventilation. The duration of lying down under the option of keeping cows using air cooling elements was higher by 68 min/day compared to keeping on deep litter without air cooling elements. In a cowshed with ventilation systems, the energy consumption for heat transfer of the body was 63.1 MJ, which is 5.4 MJ lower than in a cowshed on deep litter without the use of ventilation systems.

PRODUCTIVITY AND COMFORT OF KEEPING COWS AT LOW ENVIRONMENTAL TEMPERATURES

The aim of this work was to study the effect of low temperatures on productivity, microclimate parameters and comfort of keeping cows in different variants of prefabricated premises. The research was conducted in the central part of Ukraine (Kyiv region, Bila Tserkva district) in three farms where animals are kept loose at different sizes of prefabricated premises. The first option is a prefabricated room without insulation elements; the second prefabricated room with elements of warming of side walls by polycarbonate glass; the third in an easy-to-assemble room on a deep, long-lasting straw litter. The average annual number of dairy cows in all farms is 400. The studies were performed in the thermoneutral period, when the average daily ambient temperature did not fall below -5.0 ° C (17 days) and the period of high-temperature load, when the value of the average daily ambient temperature was -15 °C and below (11 days). It was found that prolonged low-temperature load significantly affects the comfort of cows in the options for keeping in easily assembled rooms of different types. The average daily air temperature in all variants of easily assembled rooms was negative. At the same time, during boxing without insulation elements, the temperature was -5.3 ° С, which is slightly lower than the permissible values ​​recommended for dairy cattle. The best values ​​among prefabricated premises in terms of microclimate indicators, bioclimatic indices, resting place temperatures and energy costs for heat production were for the use of polycarbonate glass insulation systems during the winter period. The use of elements of insulation of easily assembled premises provided the lowest among the studied options of premises reduction of productivity in the period of low-temperature load.

Black point of winter wheat seeds

Goal. To study infection of seeds of winter wheat varieties by «black point» and to establish the phytopathogenic composition of pathogens in the Right Bank Forest-Steppe of Ukraine.
 Methods. Laboratory — macroscopic analysis was performed according to DSTU 4138-2002, phytopathogenic composition was identified using nutrient medium, statistical analysis of the obtained data — calculation of the degree of pathogen severity, LSD.
 Results. A collection of 27 varieties of soft winter wheat was studied to determine the degree of seed infection. The highest percentage of infection was found in varieties Vodogray Bilotserkivsky (Ukraine), Adele (Russia), Balitus (Austria), Viglanka (Slovakia), Sefeg-2 (Azerbaijan). The least susceptible to the disease are varieties Daria (Croatia), Bodycek (France), Gratsia Bilotserkivska (Ukraine, Bila Tserkva DSS), Azano (Sweden), Gospodarka (Ukraine), Kozyr (Ukraine), Morozko (Russia), the number of grains with signs of darkening in the embryonic zone for these varieties did not exceed 1%. The sowing qualities of the diseased seeds were determined. The species composition of phytopathogens that cause the disease has been established. The phytopathogenic complex of seeds with the «black point» included fungi of the genera Alternaria, Fusarium, Curvularia, Bipolaris, Aspergillus, Acremoniella, Stemphillium, Sordaria and Epicoccum. Most often, the seeds were colonized by fungi of the genus Alternaria Nees. Their share in the years of research was 76.6—83.1%.
 Conclusions. The infection of seeds of winter wheat varieties by black point under conditions of the Right-Bank Forest-Steppe of Ukraine in 2018—2019 ranged from 0 to 19.8% depending on the variety and year of research. The phytopathogenic complex included 13 species of fungi from 9 genera: Alternaria, Fusarium, Curvularia, Bipolaris, Aspergillus, Acremoniella, Stemphillium, Sordaria, Epicoccum. The part of Alternaria spp. in the years of research was over 75%, the most common species were A. tenuissima and A. infectoria. The percentage of species from other genera was insignificant and did not exceed 4.6%.

Продуктивність сіяних лучих травостоїв залежно від елементів технології вирощування

The results of research on the influence of species composition of grass mixtures, the level of fertilizing, and growth stimulator Fumar on the productivity of sown meadow herbage on typical low-humus chernozems of the Right-Bank Forest-Steppe are presented. The experimental part of the work was performed in the scientific laboratories of the Forage Production, Land Reclamation, and Meteorology Department in the production unit of the National University of Life and Environmental Sciences of Ukraine "Agronomic Research Station". The territory of the research station is located in the Right-Bank Forest-Steppe and is a part of Bila Tserkva agro-soil district. The experimental plots were laid on typical low-humus chernozems, coarse-grained light loam in terms of mechanical composition, which are characterized by a high content of gross and mobile forms of nutrients. The climate of the region is characterized by unstable humidity and moderate temperatures. The average annual air temperature is 6-8 ° C. The annual amount of precipitation reaches 562 mm, during the growing season - 354-394 mm (63-70% of the annual norm), which falls unevenly throughout the year. The purpose of the study is to establish patterns of high productivity formation of sown perennial grasses depending on the species composition and fertilizers on typical low-humus chernozems of Right-Bank Forest-Steppe. The analysis of fertilizers effect showed that the highest productivity on all stands was provided by the combined application of complete mineral fertilizer and biostimulator of growth Fumar (N60P60K90 + Fumar), where productivity compared to the option without fertilizer on average over the years of research on alfalfa t/ha of dry mass or by 9%, on its mixtures with cereals - by 0.89–1.19 t/ha, or by 9–11%, and on cereal herbages - by 2.79 t/ha, or by 54% at LSD 0.41 t/ha. Productivity from the use of growth stimulant Fumar increased by 0.27-0.32 t/ha of dry weight or 2-4% and depended a little on the species composition of herbages. High efficiency of alfalfa inclusion in leguminous-cereal grass mixtures, as well as use of single-species alfalfa sowing for fodder purposes, especially on backgrounds without mineral nitrogen, was revealed. The productivity of these herbages in comparison with cereal herbages on nitrogen-free backgrounds (variants without fertilizers and P60K90) on average for 2014–2016 increased from 3.74–4.10 to 8.06–8.68 t/ha of fodder units, from 0.57-0.66 to 1.70-1.96 of crude protein, from 93.7-101.4 to 182.1-206.2 GJ/ha of gross energy and from 38.9-42.7 to 82.4–91.3 GJ/ha of metabolic energy or by1.8–3.0 times, while on backgrounds with nitrogen application (variants N60P60K90 and N60P60K90 + Fumar) - respectively from 5.73–5.93 to 8, 27–9.22 t/ha, from 1.13–1.21 to 1.89–2.29 t/ha, from 139.8–144.8 to 192.5–219.1 GJ/ha, and from 59.6–61.7 to 89.4–98.2 GJ/ha, or by 1.4–1.6 times. Comparison of the productivity of alfalfa-cereal mixtures with single-species sowing of alfalfa shows that on the same backgrounds it was at the same level with slight fluctuations. On average, in the first three years of use, the most influential factor in the yield of 1 ha of dry mass is the factor of grass cover with a share of 60%. The share of fertilizer factor is 40%. The inclusion of alfalfa in legumes and cereal mixtures, as well as the use of single-species sowing for fodder purposes compared to cereals in the background without mineral nitrogen on average for the first three years of use, increases the productivity of sown grasslands from 5.12-5.54 to 10,44–10.78 t/ha of dry weight, from 3.74–4.10 to 8.06–8.68 t/ha of feed units, from 0.57–0.66 to 1.70 1.96 of crude protein and from 38.9–42.7 to 82.4–91.3 GJ/ha of exchange energy or by 1.8–3.0 times, while on backgrounds with N60 application - from 7.28–7.59, respectively, to 9.42–9.71 t/ha, from 5.73–5.93 to 8.27–9.22 t/ha, from 1.13–1.21 to 1.89–2.29 t/ha and from 59.6–61.7 to 89.4–98.2 GJ/ha, or only by 1.4–1.6 times. Among the alfalfa-cereal herbages in the first two years of use, the most productive was the agrocenosis, the cereal part of which is represented by smooth bromegrass and English bluegrass. The lowest, but quite high productivity of alfalfa and alfalfa-grass herbages is provided without fertilizers, which on average for the first three years of use ranges from 9.95 to 10.86 t/ha of dry weight, 1.70 to 1.85 t/ha of crude protein, 82.4–85.8 GJ/ha of metabolic energy, while cereals, respectively, 5.12 t/ha, 3.74 t/ha, 0.57 t/ha, 38.9 GJ/ha.

Impact of lactic acid product on quality indices of raw meat for the smoked sausages production

Conducted research pertains to technologies of animal products processing and the development of biotechnological methods for stabilizing microbial and enzymatic biological leaven for meat.Materials and Methods. The studies were conducted in the conditions of the Research Institute of Food and Animal Processing Technologies and Laboratory of Microbiological Research Methods, Department of Microbiology of Bila Tserkva National Agrarian University. Moisture retaining capacity and bacterial contamination of beef and pork were determined.Results. The effect of processing raw meat with lactic acid product – yogurt on the moisture retaining capacity was experimentally established. With the highest titratable acidity of yoghurt of 100-110 °T, the moisture retaining capacity of pork and beef was inferior to control on 2.13 and 1.29 % and amounted to 51.88 and 62.73 %. Under a titratable acidity of lactic acid beverage of 100-110 °T with total number of lactic acid bacteria on the surface of raw meat, it was 8.1×108 and 8.5×108, which prevailed samples with acidity level of 60-70 °T on 24.5 and 24.3 %.Conclusions. The results of studies have revealed the effect of processing raw meat by yogurt with different titratable acidity on moisture retaining capacity and bacterial contamination.

Molecular Study of Echinococcus granulosus Cestodes in Ukraine and the First Genetic Identification of Echinococcus granulosus Sensu Stricto (G1 Genotype) in the Country.

Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission. Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789bp), NADH dehydrogenase 1 (602bp) and 12S rRNA (333-334bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine). Four isolates from four pigs in the Sumy region were identified as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classified as E. granulosus s.s. (G1 genotype), including one microvariant (G1A). To our knowledge, this is the first genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The finding has implications for public health as local control programmes should take into consideration different development rate of this parasite in dogs and the greater risk of the species for human infection.

Myronosets – a new productive and high-protein winter triticale cultivar, adapted to the woodlands/forest-steppe and forest-steppe of Ukraine

The creation of new triticale genotypes, the potential of which to a certain extent would meet scientific and production needs, will never lose its relevance.
 Purpose and objectives – to create a new productive and high-protein winter triticale cultivar, adapted to the Woodlands/Forest-Steppe and Forest-Steppe of Ukraine.
 Materials and methods. The breeding work to expend the triticale diversity was continued at the Educational and Research Center of Bila Tserkva National Agrarian University in 2007–2017. The control and environmental trials were conducted in 2009-2019 in the Woodlands (Polissya Institute of Agriculture NAAS (2007–2009); predecessor: seeded fallow), central Forest-Steppe (V.M. Remeslo Myronivska Institute of Wheat NAAS (2016–2019); forecrops: mustard, soybean, pea for seeds; Bila Tserkva NAU (2009–2016); predecessors: seeded fallow, potato), and northern Forest-Steppe (Institute of Horticulture NAAS (2016–2017); forecrop: perennial grasses). No mineral fertilizers were applied. No plant protection measures were taken. Hybridization was performed by manual emasculation of female components. Plants were pollinated by the twirl method 2–3 days after emasculation. The breeding material was studied in compliance with the guidelines of the All-Union Research Institute of Plant Breeding and the methodology of the state cultivar trials. Data were statistically processed, as B.А. Dospekhov described, using Statistica-5.5 and Excel-2003 software.
 Results and discussion. We focus on the importance of triticale for mankind both at the present and in the future. We retrospectively reviewed data on the creation of a new winter triticale cultivar, Myronosets. Bioecological features and morphological characteristics of the new cultivar are given. The grain yield and quality are also summarized. The article covers the quantitative parameters of dough and bread from Myronosets grain. We compared the yields, resistance to lodging, in-spike germination, resistance to fungal pathogens and unfavorable abiotic environmental factors in the new triticale cultivar and previously created ones.
 The data of the 2019–2020 state variety trials of Myronosets are analyzed, and they indicate that the new cultivar has a high potential in agroecosystems of the Woodlands and Forest-Steppe of Ukraine.
 Myronosets was found to be a source of the short stem (£90 cm) in subsequent hybrid generations.
 In 2008, interspecific hybridization within the notogenus × Triticosecale and double individual selection from F2–F4 hybrid populations derived from crossing (♀Avhusto x ♂Yahuar) x ♂K9844/93 followed by repeated improving selections in F10 allowed us to distinguish the best line, LCh97, which was later named as cultivar Myronosets.
 The new triticale cultivar can give > 7.5 t/ha. The average yield is 5.7 t/ha in the Forest-Steppe – Woodlands transition belt, 6.3 t/ha in the central Forest-Steppe, and 3.5 t/ha in the Woodlands. It is noticeable for high lodging resistance, high shedding resistance, low spike brashness, resistance to in-spike germination, complex resistance to Puccinia recondita f. sp. tritici Rob. ex Desm (8.5 points), Blumeria graminis (DC.) Speer (9 points.), and Fusarium graminearum Schwabe (8.5 points) and to to the pest Mayetiola destructor Say (8.5 points), high frost tolerance, high winter hardiness, and high drought resistance (8.5–9 points). About 70% of plants survive the laboratory freezing at -18.3°C. The cultivar is also resistant to spring and autumn frosts (9 points).
 Myronosets plants were revealed to be able to regulate their performance under the influence of unfavorable extreme bio- and abiotic factors, leaving 3–4 (more seldom 1–2) fruiting stems, often 1–3 stems with underdeveloped spikes and 1–2 unfertile stems without spikes, which form a radical rosette.
 Conclusions. As it was shown in the state variety trials, Myronosets is a mid-protein cultivar and recommended for cultivation in agroecosystems of the Woodlands and Forest-Steppe. Myronosets was proven to be a source of the short stem

PHYSICOCHEMICAL AND MICROBIOLOGICAL EXAMINATION OF RAW MILK

Introduction. Ukraine is actively implementing safety legislation and certain indicators of food quality, in particular raw milk, to the requirements of the European Union. Modern requirements for raw milk require careful analysis of hygienic indicators. Materials and methods. Raw milk materials supplied to Bila Tserkva Dairy Plant LLC and PJSC Vita Dairy Plant of Kyiv Region were studied. The number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAM) and the species composition of the milk microflora, in particular, bacteria of the genus Salmonella, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli bacteria (psychotrophs) and Physico-chemical methods were used to determine: density, mass fraction of dry matter, somatic cell content, acidity, purity group, mass fraction of protein and fat. Results and Discussion. According to research, the quality of farm milk is an order of magnitude better than milk obtained from private farms, in particular, KMAFAM. The technology of obtaining farmer's milk ensures its production of the highest and first grades, while the milk obtained in the conditions of private peasant farms – the first grade and non-grade. According to physicochemical parameters, milk obtained under different conditions did not differ significantly. Microbiological parameters differed significantly. The average number of MAFANM milk from private farms was 4361.25 ± 241.15, which is 12.6 times higher than the number of MAFANM even milk of the first grade obtained in a dairy farm. Irrespective of the season and conditions of raw milk production, all tested samples met the requirements of the current DSTU for the absence of bacteria of the genus Salmonella in 25 cm 3 , Staphylococcus aureus, 0.1 cm 3 and Listeria monocytogenes, 25 cm 3 . Bacterial bacteria were not detected in farm milk during the year, in contrast to milk from private farms, where this group of bacteria was detected in spring and autumn (20% of cases). Both in farming and in milk from private farms, a group of mesophilic microorganisms prevailed over spore-forming and psychrotrophic ones. However, their number was different. Prospects for further research are to determine the sources of various types of microorganisms in raw milk and to develop procedures to eliminate the possibility of contamination of milk with foreign microflora. Key words: raw milk, farm milk, milk from private farms, milk microflora, quality indicators

Assessment of the state of the Protoka river water system by toxicological and bioindicative indicators

Water resources are one of the most important, but at the same time the most impressible components of the environment. This component is able to change very quickly under the influence of economic and human activities. The constant growth of anthropogenic pressure on the hydrosphere, changes in water regime and surface water reserves cause to impairment of quantitative and qualitative indicators of surface water. Biological tests play a special role in assessing the environment state. The results of chemical analysis performed with the help of complex equipment, in many cases do not allow assessing the true danger of pollutants on the environment, to predict the effects of their impact on living organisms. Various pollutants, getting into the environment, can undergo transformations, while increasing their toxic effects. For this reason, methods of integrated assessment of the quality of the aquatic environment are necessary. Biotesting methods play a huge role in this. Biotestingis used for express control over industrial waste, control of technological processes in real time; to determine the level of toxicity of new products, to control the toxic effects of materials, drugs, food, to determine the quality of drinking water, etc. The methodological possibilities of studying the toxicity of various substances on test organisms have significantly expanded over the last twenty years. We are intensively searching for the most sensitive test objects and indicators. Instrumental methods of analysis are being worked out. Various methods are used to assess the quality of the aquatic environment, changes in the parameters of physiological systems and the biochemical status of test organisms, test organs.The Protoka River ecological condition in Bila Tserkva district of Kyiv region was studied by bioindication methods (study of fluctuating asymmetry of aquatic organisms’ development, growth test to establish phytotoxicity of water and coastal soil). Toxicological investigation (pH determination, TDS, content of heavy metals in the coastal soil) were set. Histological parameters of silver carp parenchymal organs were conducted. Changes in the histostructure of the liver and kidneys of silver carp were detected. They indicate unfavorable conditions for fish. The obtained results can be used for ecological monitoring of the aquatic environment.