The study deals with clarification of nanochromium citrate (NCC) effect on glucose level in the blood of animals in a wide range of doses under various regimens (single, repeated) and ways of administration. Objective: to investigate nanochromium citrate effect on glucose level in the blood of normoglycemic animals under various conditions and regimens of administration. Material and methods. The study was conducted on 84 mature male rats with the body weight of 160-180 g. During screening examination glucose concentration in the blood of intact (normoglycemic) rats was determined after NCC single administration through the peritoneum in a wide range of doses (0,22; 0,11; 0,04; 0,02; 0,01 mg/kg), different more than 20 times and were 5; 2,5; 1; 0,5; 0,25% from DL50 respectively. Blood was taken for analysis 2 hours after NCC administration. In other series hypoglycemic activity of NCC was examined after a long (14 days) administration into the peritoneum and intracutaneously. Glucose level in the blood taken from the caudal vein was determined by means of glucometer (Accu-Chek Active New, Germany). Results. After a single NCC administration into the peritoneum in the doses 0,22; 0,11 mg/kg (5; 2,5% from DL50) hypoglycemic effect was not observed, with smaller doses – 0,04; 0,02 mg/kg (1%; 0,5% from DL50) it was unreliable, and with NCC in the still smaller dose – 0,01 mg/kg (0,25% from DL50) glucose concentration in the blood decreased 17.6% (p<0,05) in comparison with the control animals. Thus, from a wide range of the examined doses the most pronounced hypoglycemic effect of NCC was found to occur in the dose of 0,01 mg/kg, which can be considered as conditionally effective. Against a background of repeated (14 days) administrations of NCC in the doses of 0,01 and 0,02 mg/kg glucose level in the blood 47,3% and 44,6% decreased respectively in comparison with the control animals. The bigger the dose of NCC (0,04; 0,11; 0,22 mg/ kg) was, the smaller glucose concentration became, less pronounced – 39,1%, 39,1%; 38,85%; and 36,7%. That is, together with a course dose increase hypoglycemic activity of NCC decreases similar to that of a single administration. Comparison of sugar reducing effect of NCC in intact animals under conditions of a single and long (14 days) administration into the peritoneum in similar doses indicated that hypoglycemic effect of NCC was more pronounced during repeated administrations (on an average 1,5 times, р<0,01). Similar to a single administration an inversely proportional dependence is seen between a sugar reducing effect and the dose given: with smaller doses (0,01 mg/kg) hypoglycemic effect of NCC was more pronounced than with bigger ones and vice-versa. After a long (14 days) administration into the stomach sugar reducing effect of NCC remains and increases analogically to administration into the peritoneum with longer introduction. On the one hand, the obtained results of hypoglycemic activity of NCC with a long administration in different ways (intracutaneously, intraperitoneally) are indicative of the lack of tolerance with repeated NCC administration. On the other hand, hypoglycemic activity is intensified with a reduced dose. Conclusions. 1. NCC demonstrates hypoglycemic action during parenteral (into the peritoneum) and enteral (intracutaneous) administrations and depends on the dose and regimens of use. The most pronounced hypoglycemic effect of NCC is found after a single administration in the dose of 0,01 mg/kg (0,25% from DL50) as conditionally effective. 2. During a long (14 days) administration into the peritoneum and intracutaneously hypoglycemic activity of NCC remains and increases. An inversely proportional dependence is observed between hypoglycemic effect of NCC and the dose given.
Read full abstract