Big Cats Research Articles

Capillary-Electrophoresis-Based Species Barcoding of Big Cats: CR-mtDNA-Length Polymorphism.

This study aimed to provide an overview of the methodological approach used for the species determination of big cats. The molecular system described herein employs mitochondrial DNA control region (CR-mtDNA)-length polymorphism in combination with highly sensitive and precise capillary electrophoresis. We demonstrated that the described CR-mtDNA barcoding system can be utilized for species determination where the presence of biological material from big cats is expected or used as a confirmatory test alongside Sanger or massive parallel sequencing (MPS). We have also addressed the fact that species barcoding, when based on the analysis of mtDNA targets, can be biased by nuclear inserts of the mitochondrial genome (NUMTs). The CR-mtDNA barcoding system is suitable even for problematic and challenging samples, such as hair. CR-mtDNA-length polymorphisms can also distinguish hybrids from pure breeds.

NEW RECORDS OF HELMINTHS OF THE JAGUAR IN MEXICO, WITH AN UPDATED LIST OF SPECIES IN THE AMERICAS.

An inventory of parasites infecting the jaguar (Panthera onca) across its distribution range is relevant for the conservation of this threatened big cat. In this study, we report the occurrence of helminths in a jaguar from Mexico using morphological techniques (cleared and stained mounts and scanning electron microscopy) and partial sequences of the 28S ribosomal RNA (28S rRNA) gene and the cytochrome c oxidase 1 mitochondrial (COI) gene. We also provide an updated list of helminth species reported in jaguars in the Americas. Three helminth taxa are identified in the jaguar examined from Mexico: Toxocara cati, Physaloptera sp., and Taenia sp. The new 28S rRNA sequences of To. cati, Physaloptera sp., and Taenia sp. and the COI sequence of Taenia sp. corroborate the identity of the helminths isolated from this host. One hundred and twenty-nine records of helminths parasitizing jaguars from 49 studies up to May 2023 were identified in the Americas. In most of these studies (73.6%), helminths were identified using coproparasitological techniques. Sixteen helminths (7 nematodes, 5 cestodes, 3 acanthocephalans, and 1 trematode) were identified at the species level in free-ranging and captive jaguars. The study demonstrates the value of an integrative taxonomy approach to increase the accuracy of parasite identification in wildlife, especially when helminth specimens are scarce or poorly fixed.

Can Non-invasive Hormone Profiling in Female Tigers (Panthera tigris) could Differentiate True Pregnancy and Pseudopregnancy

Background: The tigers (Panthera tigris) being the world’s largest cat are one of the critically endangered species which are at the verge of extinction. The pseudopregnancy or false pregnancy or pseudocyesis is a syndrome which is characterized by the presence of signs of pregnancy in a non-pregnant animal. False lactation or nervous lactation are the terms which are used to refer pseudopregnancy as there is very low quantities of milk production is seen. This condition can be differentiated with true pregnancy by using hormones and by correlation of behavioral symptoms of pregnancy with hormonal profiles for confirmation of pregnancy in female tigers. Methods: Total seven animals were used in the experiment and was divided into three groups (G I, G II and G III). Group G I consist of 03 non-bred animals of less than 9 years of age, G II consisted of 03 non-bred animals of more than 9 years of age and G III was consisted of 03 animals after mating irrespective of their age. Faecal samples were collected non-invasively from enclosures weekly up to 14 weeks. Only fresh samples (deposited within 24 h) was chosen, urine puddles were aspirated from the floor. The extracted samples were then analysed for estimation of relaxin, progesterone and prostaglandin F2 alpha metabolite (PGFM) by enzyme linked immunosorbent assay (ELISA). Result: Relaxin the hormone for early detection of pregnancy in felines gives elevated concentration from 49 days after mating and attains a peak during parturition, whereas, no elevation or peak was observed in pseudopregnant animals. Progesterone, in true pregnancy remains elevated from 7th week onwards after mating, it gets dropped after 7th week with wide fluctuation in pseudopregnant animals. PGFM elevation observed after nine weeks of gestation in true pregnancy whereas, no elevation will be observed in pseudopregnant tigress.

Spatial population distribution dynamics of big cats and ungulates with seasonal and disturbance changes in temperate natural forest

Wildlife conservation and management in human-dominated landscapes are major concerns for wildlife ecologists and managers. The dynamics of human disturbance, combined with seasonal limitations in the availability of nutritious foods, may restrict wildlife population growth and recovery. However, understanding how large mammal species adjust their population distribution in forest habitats with seasonal changes in food and disturbances requires a deeper and more extensive analysis. In this study, we found that three ungulate species, roe deer (Capreolus pygargus), sika deer (Cervus nippon), and wild boar (Sus scrofa), employ robust, conservative, and flexible distribution strategies, respectively, to adapt to the effects of seasonal changes and human disturbances. Moreover, croplands, villages, and grazing have some negative effects on the distribution of roe deer and sika deer, while wild boar can be highly abundant near human land use. Additionally, roe deer, sika deer, and wild boar are also affected by the abundance of shrub species they consume. During the cold season, the populations of the Amur tiger (Panthera tigris altaica) and Amur leopard (P. pardus orientalis) were primarily located near roads and dense forests, respectively. In the warm season, the distribution of both big cats was influenced by prey abundance, and Amur tigers also avoided grazing livestock. Nevertheless, the negative effects of human land use on Amur tigers and wild boars increased during the warm season, which was attributed to more frequent human activities during that time. Consequently, it is crucial to implement season-specific habitat management, particularly by regulating human disturbances during the warm season, in order to promote the recovery and expansion of populations of big cats and ungulates.

Can the puma Messi, the cheetah Gerda, the panther Luna, the wolf Kira be considered pets? (man in culture and the animal world)

The article discusses a new trend to have wild animals (big cats, foxes, ferrets, etc.) in some homes, and it turns out that since they have been raised since childhood, loved and cared for, they behave like pets (dogs and cats). The question is whether these animals can still be considered domestic or not. To solve this problem, characteristics are given that make it possible to distinguish between wild and domestic animals, and the question is raised whether they can be applied to this case. To understand this new class of animals, the concept of "anthropomorphic" is introduced and it is shown that their psyche differs from that of wild animals, it is closer to domestic ones. An explanation is offered for the experiments of geneticist Dmitry Belyaev on the domestication of silver foxes. The author argues that the evolution of foxes was influenced by two factors, not only genetic selection, as Belyaev believed, but also the impact of the environment created by man, an environment conducive to the formation of anthropoids. Attention is drawn to the fact that humans continue to crowd large wild animals. Humans increasingly need land for goats, cows, horses, pigs, poultry, and agricultural land. The paradox ‒ our big cats of anthropoids are fed the meat of these pets. At the end of the article, Belyaev's hypothesis, according to which we are "self-domesticated" monkeys, whose innate psychological tendencies, behavior and social structure have radically changed under the influence of selection for reduced aggressiveness, is compared with the author's hypothesis about the role of signs in human origin. He shows that domestication occurred as a result of the transition of hominids to "paradoxical behavior", which required the creation of a sign based on a signaling system. The latter allowed the hominids to act contrary to biological evidence at the command of the leader. A necessary condition for this was the imagination conditioned by signs, which helped to invent the first tools.

Small Felids Coexist in Mixed-Use Landscape in the Bolivian Amazon.

In the face of global species loss, it is paramount to understand the effects of human activity on vulnerable species, particularly in highly diverse, complex systems. The Greater Madidi Landscape in the Bolivian Amazon includes several biodiverse protected areas that were created with the goal of sustaining healthy and diverse ecosystems while not impeding the livelihoods of local indigenous peoples. In this study, we sought to use camera trap data and single-species occupancy analysis to assess the impacts of different forms of human activity on four species of small felids: ocelots (Leopardus pardalis), margays (Leopardus wiedii), jaguarundi (Herpailurus yagouaroundi), and oncilla (Leopardus tigrinus). We modeled both human variables (proximity to indigenous communities, roads, and tourist camps) and non-human variables (terrain ruggedness, proximity to rivers, canopy height, prey availability, and large cat abundance). Margay occupancy was unaffected by any of these human variables and ocelots showed only weak evidence of being affected by tourism. Ocelots were particularly pervasive throughout the study area and were consistently estimated to have high occupancy probability. We did not obtain sufficient data on jaguarundi or oncilla to reliably model these effects. Our results indicate that small cats successfully coexist both with each other and with the surrounding human activity in this unique landscape, which serves as a model for global protected area management.

Leopards (Panthera pardus pardus) in Gashaka Gumti National Park, Nigeria: historical records and recent sightings

West-African populations of the African subspecies of leopards (Panthera pardus pardus) are very fragmented, particularly so in Nigeria, Africa’s most populous nation. We compile historical and recent information about the occurrence of these big cats in what is likely their last remaining stronghold: Gashaka Gumti National Park (GGNP) in Adamawa and Taraba States along the border with Cameroon. Leopards in GGNP’s northern Gumti sector are close to extinction, given that its open plains of savannah are heavily encroached upon by illegal cattle grazing. However, in GGNP’s southern Gashaka sector, leopards are better protected, given its often mountainous and densely forested landscape. We present specific data for a 30 km2 forest-savannah area around a research station at Kwano, which harbours sizeable populations of 35 species of prey species leopards target elsewhere. Here, direct evidence for leopard presence (e.g., footprints, scratchmarks, predation incidences, vocalizations, sightings, cameratrap images) amounts to 1 per year. Albeit this encounter rate is very low, it is relatively steady throughout the last 30 years and into the present. We assume that GGNP’s Gashaka sector represents one of the best chances for leopards to survive in Nigeria.

Audience segmentation of New Zealand cat owners: Understanding the barriers and drivers of cat containment behavior.

Free-roaming companion cats have a detrimental impact on the environment and are at risk of harm. Despite these negative impacts, it is the norm in New Zealand (NZ) to allow companion cats to roam freely and only a minority of cat owners practice cat containment. This study firstly sought to identify what factors act as barriers and drivers of NZ owners' participation in cat containment, and secondly whether NZ owners could be segmented into unique audiences based on the factors predicting their cat containment behavior. It was hypothesized that cat owners with greater capability, opportunity, and motivation to perform cat containment would have greater cat containment intentions and behavior. Furthermore, it was expected that at least three segments of cat owners would exist in NZ which differed significantly in the set of capability, opportunity and motivational factors predicting their cat containment behavior. A quantitative online cross-sectional survey of 395 NZ cat owners was conducted, measuring containment intentions and behavior, and capability, opportunity, and motivation to perform cat containment. Results from bivariate correlations and multiple regression demonstrated that capability, opportunity, and motivational factors predicted increased cat containment intentions and behavior. Latent profile analysis identified four distinct segments of cat owners with unique COM profiles; engaged (6%), receptive (17%), ambivalent (48%), and opposed (30%). Validation analysis demonstrated that these groups all differed significantly in their cat containment intentions and behaviors. From these findings theoretically grounded behavior change interventions can be developed to target the causes of non-participation in cat containment for each of the identified cat owner segments, thereby improving the management of free-roaming cats in NZ.

The genome of the black-footed cat: Revealing a rich natural history and urgent conservation priorities for small felids.

Habitat degradation and loss of genetic diversity are common threats faced by almost all of today's wild cats. Big cats, such as tigers and lions, are of great concern and have received considerable conservation attention through policies and international actions. However, knowledge of and conservation actions for small wild cats are lagging considerably behind. The black-footed cat, Felis nigripes, one of the smallest felid species, is experiencing increasing threats with a rapid reduction in population size. However, there is a lack of genetic information to assist in developing effective conservation actions. A de novo assembly of a high-quality chromosome-level reference genome of the black-footed cat was made, and comparative genomics and population genomics analyses were carried out. These analyses revealed that the most significant genetic changes in the evolution of the black-footed cat are the rapid evolution of sensory and metabolic-related genes, reflecting genetic adaptations to its characteristic nocturnal hunting and a high metabolic rate. Genomes of the black-footed cat exhibit a high level of inbreeding, especially for signals of recent inbreeding events, which suggest that they may have experienced severe genetic isolation caused by habitat fragmentation. More importantly, inbreeding associated with two deleterious mutated genes may exacerbate the risk of amyloidosis, the dominant disease that causes mortality of about 70% of captive individuals. Our research provides comprehensive documentation of the evolutionary history of the black-footed cat and suggests that there is an urgent need to investigate genomic variations of small felids worldwide to support effective conservation actions.

How to Eat Spiny Food? Predation by Bird of Prey on Thin-Spined Porcupine, Chaetomys subspinosus (Olfers, 1818)

Chaetomys subspinosus is an arboreal, cryptic, medium-sized porcupine. This species has the body densely covered by spines thinner and softer than those of other genera of Neotropical porcupines. Its known natural predators are big cats and harpy eagle, while other mammalian carnivores and large birds of prey in general are cited as potential predators. Here I report a new event of predation by bird of prey on Chaetomys subspinosus in the northern Espírito Santo state, southeastern Atlantic Forest. The predator removed the stomach and intestine of the prey, as well as the spines of its head and the ventral portion of the body. Although it was not possible to identify the predator precisely, it was a medium to large-sized bird of prey, probably a hawk or a hawk-eagle (Family Accipitridae). The presented data contributes information to the natural history of Chaetomys subspinosus, representing one of the first records of predation on this porcupine species by a bird of prey.

Habitat accessibility and snares impact large cats and their prey in Northeast Tiger and Leopard National Park, China

Snares have become a widespread hunting technique, with their indiscriminate nature jeopardizing terrestrial large carnivores. Since the lack of reliable data on snare distribution hampers an accurate evaluation of the potential risk. Continuous winter SMART (the Spatial Monitoring and Reporting Tool) patrols have operated in the Amur tiger (Panthera tigris altaica) and Amur leopard (Panthera pardus orientalis) ranges in northeast China from 2013 to 2020 to record wildlife occurrences and remove snares. Our research aimed to assess the snares threat pose in the Amur tiger range and whether snare removal benefited roe deer (Capreolus pygargus) and wild boar (Sus scrofa) recovery. We modeled the spatial distribution of Amur tigers, Amur leopards, and prey species, comparing their distributions before and after anti-poaching measures. We also assessed the overlap between Amur tigers, Amur leopards, and snare locations. Additionally, we used the MaxEnt model to predict snare distribution, and then we analyzed the overlap of snares with species at different periods between 2013 and 2015 and 2018–2020.Our results showed that the probability of occurrence of ungulates increased significantly around roads after snares were removed. Furthermore, we found that Amur tigers and Amur leopards distribution overlapped with snare locations suggesting that snares pose a serious risk to these non-target species. The overlap between snares and species has the same trend as the growing with species distribution, and the least significant increase is in DHZ-XNC.Removing snares not only aids target game species but also protects sympatric large carnivores.

Getting to the Meat of It: The Effects of a Captive Diet upon the Skull Morphology of the Lion and Tiger

Zoo animals are crucial for conserving and potentially re-introducing species to the wild, yet it is known that the morphology of captive animals differs from that of wild animals. It is important to know how and why zoo and wild animal morphology differs to better care for captive animals and enhance their survival in reintroductions, and to understand how plasticity may influence morphology, which is supposedly indicative of evolutionary relationships. Using museum collections, we took 56 morphological measurements of skulls and mandibles from 617 captive and wild lions and tigers, reflecting each species’ recent historical range. Linear morphometrics were used to identify differences in size and shape. Skull size does not differ between captive and wild lions and tigers, but skull and mandible shape does. Differences occur in regions associated with biting, indicating that diet has influenced forces acting upon the skull and mandible. The diets of captive big cats used in this study predominantly consisted of whole or partial carcasses, which closely resemble the mechanical properties of wild diets. Thus, we speculate that the additional impacts of killing, manipulating and consuming large prey in the wild have driven differentiation between captive and wild big cats.

Social media data from two iconic Neotropical big cats: can this translate to action?

IntroductionThere has been a gradual increase in studies of social media data usage in biodiversity conservation. Social media data is an underused source of information with the potential to maximize the outcomes of established conservation measures. In this study, we assessed how structured social media data can provide insight into species conservation through a species conservation plan, based on predefined actions. MethodsWe established a framework centered on a set of steps that go from defining social media platforms and species of interest to applying general analysis of data based on data dimensions—three W’s framework (What, When, Who) and the public engagement that posts received. The final and most important step in our proposed framework is to assess the overlap between social media data outcomes and measures established in conservation plans. In our study, we used the Brazilian National Action Plan (BNAP) for big cats as our model. We extracted posts and metrics about jaguars (Panthera onca) and pumas (Puma concolor) from two social media platforms, Facebook and Twitter. ResultsWe obtained 159 posts for both jaguars and pumas on Facebook (manually) and 23,869 posts for the jaguar and 14,675 posts for the puma on Twitter (through an application user interface). Data were categorized for content and users (only Facebook data) based on analysis of the content obtained and similarities found between posts. We used descriptive statistics for analyzing the metrics extracted for each data dimension (what, when, who, and engagement). We also used algorithms to predict categories in the Twitter database. Our most important findings were based on the development of a matrix summarizing the overlapping actions and dimensions of the data. Our findings revealed that the most prominent category of information for jaguars on Facebook was the sighting of wildlife outside protected areas, while for pumas, it was the trespassing of property by wildlife. From the Twitter dataset, we observed that the most prominent category of information for jaguars was: the sighting of wildlife outside protected areas, while for pumas, it was wildlife depredation by direct or indirect means. We found temporal trends that highlight the importance of categories in understanding information peaks on Facebook and Twitter. DiscussionWhen we analyze online engagement, we see a predominance of positive reactions on Facebook, and on Twitter, we see a balanced reaction between positive and negative. We identified 10 of 41 actions in the BNAP that might benefit from social media data. Most of the actions that could benefit from our dataset were linked to human–wildlife conflicts and threats, such as wildlife–vehicle collisions. Communication and educational actions could benefit from all dimensions of the data. Our results highlight the variety of information on social media to inform conservation programs and their application to conservation actions. We believe that studies on the success of applying data to conservation measures are the next step in this process and could benefit from input from decision-makers.

Large carnivore distribution, conflicts and threats in the east of the Somali region, Ethiopia

AbstractLarge carnivore distribution in Ethiopia is not well documented; we did 108 interviews and 12 focus group discussions in six districts of the Somali Region to gather basic information. The Region is not listed as resident range for big cats, but our results indicate widespread presence of cheetah, and possible remnants of lion and leopard occurrence. Spotted and striped hyaena, plus many smaller species, are frequently encountered. In contrast to positive attitudes towards wild grazers, all carnivores are persecuted in response to frequent conflict leading to injuries and deaths among people and livestock. Lion and cheetah cubs were regularly captured and trafficked, and this may have contributed to lion declines especially. Cheetah cubs are still regularly caught and we mapped how they are smuggled to Somalia/Somaliland from where they enter the pet trade.

Retrospective study of the incidence of dental pathologies in big cats under human care in Guatemala, Central America

Retrospective study of the incidence of dental pathologies in big cats under human care in Guatemala, Central America

How genomic insights into the evolutionary history of clouded leopards inform their conservation.

Clouded leopards (Neofelis spp.), a morphologically and ecologically distinct lineage of big cats, are severely threatened by habitat loss and fragmentation, targeted hunting, and other human activities. The long-held poor understanding of their genetics and evolution has undermined the effectiveness of conservation actions. Here, we report a comprehensive investigation of the whole genomes, population genetics, and adaptive evolution of Neofelis. Our results indicate the genus Neofelis arose during the Pleistocene, coinciding with glacial-induced climate changes to the distributions of savannas and rainforests, and signatures of natural selection associated with genes functioning in tooth, pigmentation, and tail development, associated with clouded leopards' unique adaptations. Our study highlights high-altitude adaptation as the main factor driving nontaxonomic population differentiation in Neofelis nebulosa. Population declines and inbreeding have led to reduced genetic diversity and the accumulation of deleterious variation that likely affect reproduction of clouded leopards, highlighting the urgent need for effective conservation efforts.

Big-cat hunting in the Bronze and Iron Ages of the Near East: a view from Tel Burna

In ancient Near Eastern iconography, panthers and lions were frequently used to express social status. The zooarchaeological remains of panthers and lions found in this region, however, are most commonly interpreted only as evidence for the management of dangerous animals. Starting with the faunal material from Iron Age Tel Burna, the authors collate and analyse zooarchaeological evidence for big cats across the Near East, from the Neolithic to the Iron Age (c. 9500–50 BC). The results show a shift in assemblage composition and find contexts starting in the Chalcolithic period, indicating the display of these animals by political leaders. The results also urge caution in the use of archaeological remains for reconstructing the natural ranges of big cats.

Genetic Diversity of the Amur Tiger (Panthera tigris altaica): Are There Differences between the 20th and the Early 21st Centuries?

Polymorphism of nine microsatellite loci in the Sikhote-Alin tiger population was analyzed in the modern and recent historical periods, using blood, excrement, and museum bone samples. Tests for excess heterozygosity to determine whether the population went through a period of low abundance and a low value of the Garza–Williamson coefficient indicated that such events were highly probable to occur in both recent and earlier history. The mean effective population size Ne of a current sample was 34.4 (95% confidence interval 17–106.8). This fact is of great interest in the contest of conservation and restoration of endangered large cat species.

How jaguars are actually stolen: Big cat conservation and the green extractivism nexus in the Yucatán Peninsula, Mexico

This article explores the seemingly unlikely intersection of jaguar conservation and green extractivism in the Yucatán Peninsula, Mexico. The 'green' or environmental aspect of jaguar conservation, we argue, is largely manufactured, employing the qualities of ecosystems to market and advance forms of 'place-based' extraction. Analyzing jaguar conservation programs through green extractivism not only shows problems related to market-based conservation schemes, but how these conservation projects advance spatial legibility of resources and political control over territories for public and private actors, aiding capitalist and extractive trajectories. Drawing on fieldwork in Laguna Om, an ejido––rural community––in the south of the Peninsula, the article reveals how an entanglement of market-based instruments (MBIs), voluntary carbon markets, NGOs, corporate financing, environmental policy, and technological developments continue to consume ecosystems into circuits of capitalist control and accumulation. Finally, the article demonstrates, jaguar conservation serves to greenwash traditional extractive and infrastructural development projects, such as Tren Maya. Digitalized and market-based forms of conservation, moreover, reduce ecosystems to units of accounting that are fit for trade in international markets, advancing the capture and accumulation of value through conservation, enacting a process of green extractivism.

Trabecular bone morphology in big cats reflects the complex diversity of limb use but not home range size or daily travel distance.

A relationship exists between mechanical loading and bone morphology. Although studies show a relationship between trabecular bone morphology and locomotor strategy in mammals, none of them have studied trabecular bone morphology in felid species occupying disparate and overlapping habitats. We investigate trabecular bone volume fraction (BVF) in the femoral and humeral heads, and distal tibia of four felid species (mountain lions, jaguars, cheetahs, and leopards) to identify whether there is a relationship between BVF and locomotor behavior. This study's goals are to identify whether felid species with high daily travel distance or large home range size have greater BVF compared with those with small daily travel distance or home range size, and whether BVF is correlated among the three elements of the fore and hindlimb studied. We quantified BVF in micro- and peripheral computed tomography images and found no significant differences across species in the femoral and humeral head (p > 0.05). However, in the distal tibia, results showed that leopards, mountain lions, and cheetahs have significantly greater (p < 0.05) BVF than jaguars. Despite differences in home range size and daily travel distance, the proximal elements did not reflect differences in BVF; however, the distal-most element did, suggesting decreased loading among jaguars. These findings suggest that the observed pattern of trabecular bone morphology is potentially due to the diversity in locomotor strategy of the forelimb. Additionally, these results imply that neither home range size nor daily travel distance are clear indicators of activity levels. A cautious approach is warranted in studying how loading influences trabecular morphology.