Bifidobacterium Sp Research Articles

Artisanal buffalo milk curd from Charfassion upazila of Bholadistrict in Bangladesh as a potent source of bifidobacteria

The present study explored the traditionally prepared fermented buffalo milk curd to isolate and characterize Bifidobacterium sp. towards developing future probiotics. Standard methods have been applied to identify Bifidobacterium sp. using bifidobacteria selective media, bifidobacteria specific mupirocin resistance test, phosphoketolase activity and commercial biochemical kit. The strains further confirmed by xfp gene PCR amplification. The growth dynamics of the identified isolates was also determined by monitoring the turbidity of the cultured media spectrophotometrically. A total of 20 isolates obtained from selective media were found gram positive, catalase negative, rod or v-shaped, non-motile and identified as presumptive bifidobacteria. All the isolates were resistant (zone diameter <10 mm) to the antibiotic mupirocin. However, the phosphoketolase activity of the isolates were varied significantly (p<0.05). A high, moderate and weak/no phosphoketolase activity was exhibited by 35%, 20% and 45% isolates, respectively. In the contrary, fourteen isolates (70%) amplified well with phosphoketolase activity encoded xfp gene and produced a PCR product of 704 bp. Combining the findings of these three tests, only six isolates (30%) displayed mupirocin resistance, high phosphoketolase activity and amplification with xfp gene. Of these six isolates biochemical test confirmed three isolates as Bifidobacterium mongoliense and two as Bifidobacterium pseudolongum subsp. globosum. The exponential phase growth rate of the six isolates also varied significantly (p<0.05) and ranged from 0.16±0.01/h to 0.34±0.02/h. Traditionally prepared buffalo milk curd from Charfassion upazila of Bhola district, Bangladesh could be a potent source of Bifidobacterium sp. However, species-specific identification and elucidation of probiotic potential are pivotal for future industrial use.

Multi-strain probiotics (Hexbio) containing MCP BCMC strains improved constipation and gut motility in Parkinson's disease: A randomised controlled trial.

We determined the effectiveness of a multi-strain probiotic (Hexbio®) containing microbial cell preparation MCP®BCMC® on constipation symptoms and gut motility in PD patients with constipation. PD patients with constipation (ROME III criteria) were randomized to receive a multi-strain probiotic (Lactobacillus sp and Bifidobacterium sp at 30 X 109 CFU) with fructo-oligosaccaride or placebo (fermented milk) twice daily for 8 weeks. Primary outcomes were changes in the presence of constipation symptoms using 9 items of Garrigues Questionnaire (GQ), which included an item on bowel opening frequency. Secondary outcomes were gut transit time (GTT), quality of life (PDQ39-SI), motor (MDS-UPDRS) and non-motor symptoms (NMSS). Of 55 recruited, 48 patients completed the study: 22 received probiotic and 26 received placebo. At 8 weeks, there was a significantly higher mean weekly BOF in the probiotic group compared to placebo [SD 4.18 (1.44) vs SD 2.81(1.06); (mean difference 1.37, 95% CI 0.68, 2.07, uncorrected p<0.001)]. Patients in the probiotic group reported five times higher odds (odds ratio = 5.48, 95% CI 1.57, 19.12, uncorrected p = 0.008) for having higher BOF (< 3 to 3-5 to >5 times/week) compared to the placebo group. The GTT in the probiotic group [77.32 (SD55.35) hours] reduced significantly compared to placebo [113.54 (SD 61.54) hours]; mean difference -36.22, 95% CI -68.90, -3.54, uncorrected p = 0.030). The mean change in GTT was 58.04 (SD59.04) hour vs 20.73 (SD60.48) hours respectively (mean difference 37.32, 95% CI 4.00, 70.63, uncorrected p = 0.028). No between-groups differences were observed in the NMSS, PDQ39-SI, MDS-UPDRS II and MDS-UPDRS III scores. Four patients in the probiotics group experienced mild reversible side effects. This study showed that consumption of a multi-strain probiotic (Hexbio®) over 8 weeks improved bowel opening frequency and whole gut transit time in PD patients with constipation.

Lactic Acid Bacterial Production of Exopolysaccharides from Fruit and Vegetables and Associated Benefits

Microbial polysaccharides have interesting and attractive characteristics for the food industry, especially when produced by food grade bacteria. Polysaccharides produced by lactic acid bacteria (LAB) during fermentation are extracellular macromolecules of either homo or hetero polysaccharidic nature, and can be classified according to their chemical composition and structure. The most prominent exopolysaccharide (EPS) producing lactic acid bacteria are Lactobacillus, Leuconostoc, Weissella, Lactococcus, Streptococcus, Pediococcus and Bifidobacterium sp. The EPS biosynthesis and regulation pathways are under the dependence of numerous factors as producing-species or strain, nutrient availability, and environmental conditions, resulting in varied carbohydrate compositions and beneficial properties. The interest is growing for fruits and vegetables fermented products, as new functional foods, and the present review is focused on exploring the EPS that could derive from lactic fermented fruit and vegetables. The chemical composition, biosynthetic pathways of EPS and their regulation mode is reported. The consequences of EPS on food quality, especially texture, are explored in relation to producing species. Attention is given to the scientific investigations on health benefits attributed to EPS such as prebiotic, antioxidant, anti-inflammatory and cholesterol lowering activities.

High-rate mesophilic hydrogen production from food waste using hybrid immobilized microbiome

This study examined the feasibility of dark fermentative biohydrogen production from food waste using hybrid immobilization in mesophilic condition. Among four different organic loading rates (OLRs), the highest average hydrogen production rate (HPR) of 9.82 ± 0.30 L/L-d was found at an OLR of 74.7 g hexose/L-d, which was higher than reported values from particulate feedstock in mesophilic condition. The average hydrogen yield (HY) at the condition was 1.25 ± 0.04 mol H2/mol hexoseconsumed. Whereas the average HPR and HY at an OLR 80 g hexose/L-d were 5.82 ± 0.12 L/L-d and 0.64 ± 0.02 mol H2/mol hexoseconsumed, respectively. Metabolic flux analysis showed the low HY was concurrent with the highest propionic acid and homoacetogenis. Bacterial population was shift from Clostridium sp. to non-hydrogen producers including Bifidobacterium, Bacteriodes, Olsenella, Dysgonomonas, and Dialister sp.

Antibiotic-induced changes in the human gut microbiota for the most commonly prescribed antibiotics in primary care in the UK: a systematic review.

ObjectiveThe gut microbiota influences many aspects of human health. We investigated the magnitude and duration of changes in gut microbiota in response to antibiotics commonly prescribed in UK primary care.MethodsWe searched MEDLINE, EMBASE and AMED, all years up to May 2020 including all study designs, collecting and analysing data on the effect of antibiotics prescribed for respiratory and urinary tract infections. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses and Cochrane standard methods. Risk of bias was evaluated using the Critical Appraisal Skills Programme. Narrative synthesis was used to report the themes emerging from the data.Main outcome measuresPrimary outcomes were antibiotic-induced changes in the composition and/or diversity of the gut microbiota. Secondary outcome was the time for the microbiota to return to baseline.ResultsThirty-one articles with low or unclear risk of bias showed that antibiotics impact the gut microbiota by causing rapid and diminished levels of bacterial diversity and changes in relative abundances. After cessation of treatment, gut bacteria recover, in most individuals, to their baseline state within a few weeks. Some studies suggested longer term effects from 2 to 6 months. Considerable heterogeneity in methodology makes the studies prone to biases and other confounding factors. Doxycycline was associated with a marked short-term decrease in Bifidobacterium diversity. Clarithromycin decreased the populations of Enterobacteria, and the anaerobic bacteria Bifidobacterium sp and Lactobacillus sp in numbers and diversity for up to 5 weeks. Phenoxymethylpenicillin, nitrofurantoin and amoxicillin had very little effect on the gut microbiome.ConclusionsDespite substantial heterogeneity of the studies and small sample sizes, there is evidence that antibiotics commonly used in primary care influence the composition of the gastrointestinal microbiota. Larger population-based studies are needed to fully understand how antibiotics modulate the microbiota, and to determine if these are associated with (longer term) health consequences.PROSPERO registration numberCRD42017073750.

Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria.

Short-chain cello-oligosaccharides (COS; degree of polymerization, DP ≤ 6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show the intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %), and DP 6 (9 wt %) and involved minimal loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yield), the COS were examined for growth promotion of probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides, and cellobiose, COS showed up to 4.1-fold stimulation of cell density for Clostridium butyricum, Lactococcus lactis subsp. lactis, Lactobacillus paracasei subsp. paracasei, and Lactobacillus rhamnosus but were less efficient with Bifidobacterium sp. This study shows the COS as selectively functional carbohydrates with prebiotic potential and demonstrates their efficient enzymatic production.

Influence of Probiotic Bacteria Pediococcus sp and Bifidobacterium sp on Immune System

Probiotic organisms influence the intestinal health. It helps in the maturation of the immune system, the development of normal intestinal morphology, maintains the load of intestinal flora, etc. It prevents the attachment of pathogenic microorganisms and the entry of allergens into the intestine. Probiotics improve the immune status of the individuals, which was detected by checking the levels of IgG and IgM for E. coli infection on an experimental animal fed with a probiotic drink prepared using Pediococcus sp and Bifidobacterium sp for one month. Control animals showed an increase in the level of Immunoglobulins. Both IgG and IgM, indicated the level of infection, whereas test animals did not show any increase in the level of Immunoglobulins.

Probiotics: Promise, Evidence, and Hope

Probiotics: Promise, Evidence, and Hope

Viability of MCF7 Cells Exposed to Basolateral Secretion from CaCo2 Cells Pretreated with Fecal Waters from Breast Cancer Patients and Controls

Abstract Objectives There is increasing evidence suggesting that microbiota may play a role in breast cancer disease and influence the disease severity. Several mechanisms may be involved in this relationship. Our hypothesis is that the role of microbiota in the disease may be at least partly related to its influence in gut lipid and lipoprotein metabolisms. This hypothesis was tested in an in vitro model combining MCF7 and Caco2 cells. Methods 32 women newly diagnosed for breast cancer, before any treatment and 28 apparently healthy women provided their stools from which bacterial DNA was extracted and amplified by qPCR, targeting 16S rRNA sequences specific to bacterial groups. Fecal waters (FW) were also obtained from these stools. Intestinal Caco-2 cells grown on filter inserts were incubated apically with 10% FW for 24 h. Then, MCF-7 cells were incubated with the whole basolateral medium for 24 h. The viability of these cells was estimated by MTT test. In parallel, LXR, apolipoproteins AIV and E gene expression was estimated by RT QPCR in CaCo2 cells and short chain fatty acids (SCFA) were quantified in FW. A logistic regression model was used to establish the Odds ratios (OR) for the disease of MCF7 viability and CaCo2 gene expression. The relationship between % bacterial groups, CaCo2 gene expression, SCFA and viability was established by regression models. Results Patients and controls differed by the MCF7 viability (1.05 [1.01–1.10], p = 0.04) and a tendency towards a difference was observed for apo AIV gene expression (0.63 [0.39–1.01], p = 0.055), (OR [5th-95th]). Viability was positively correlated with % Bifidobacterium sp. (21.18 ± 7.66, p = 0.008) and negatively correlated with valerate (−2.849 ± 1.048, p = 0.009), (ß±s.d.). These correlations were maintained in a multiple regression model. Conclusions Microbiota may interact with intestine cell lipid metabolism and therefore influence cancer disease through gut cell secretion or permeability. Funding Sources Ligue Contre le cancer and private funds from Integrated Center for Oncology.

Probiotics, Prebiotics and Synbiotics for Nile tilapia: Growth performance and protection against Aeromonas hydrophila infection

The use of probiotics, prebiotics and synbiotics provides better growth rates, improves the innate immune system and protection against several bacterial pathogens. The species Aeromonas hydrophila causes high mortality rate and production loss, provoking an antimicrobial overuse. This study aimed to prove the capacity of commercial probiotic DBA® (Bifidobacterium sp, Lactobacillus acidophilus and Enterococcus faecium), prebiotics MOS and chitosan, combined as synbiotics or not to promote Nile tilapia growth performance, innate immune system modulation and protection against A. hydrophila. One experiment with 24 units, duration of 63 days, and another experiment infection assay with A. hydrophila (18 experimental units, individual recirculation system, duration of 15 days) were performed. The treatments were: control, probiotic DBA (PRO), the prebiotics mannan oligosaccharides (Actigen®) (MOS) and chitosan (CHI), and the synbiotics composed of probiotic and MOS (Actigen®) (PRO + MOS), probiotic and chitosan (PRO + CHI). Growth performance, hematology, intestinal histology and immunology, and relative protection level against A. hydrophila were analyzed. Significant differences were observed in the final biomass for the treatments PRO + CHI and CHI when compared with PRO + MOS, and greater individual weight gain in group PRO + CHI in relation to group PRO + MOS. High concentrations of Lactobacillus spp. in intestinal tract of probiotics, prebiotics and symbiotics groups were observed. The treatments PRO + MOS, PRO + CHI and PRO promoted apparent preservation of the intestinal epithelium and stimulated an increase in goblet cells, blood vessels and mast cells, associated to immune system. The symbiotics MOS and DBA® promoted better relative protection level with percentage of 40%. This study recommends the use of probiotics, prebiotics and synbiotics to improve the protection against A. hydrophila infection without growth reduction. Inclusion of feed additives may reduce the application of antimicrobials in fish farming.

Comparative study on four amylosucrases from Bifidobacterium species

Amylosucrase (ASase) is α-glucan-producing enzyme. Four putative ASase genes (bdas, blas, bpas, and btas) were cloned from Bifidobacterium sp. and expressed in Escherichia coli. All ASases from Bifidobacterium sp. (BAS) displayed typical ASase properties with slightly different characteristics. Among the BASs studied, BdAS and BpAS showed maximal enzyme activities at 35 and 30 °C, respectively, whereas BlAS and BtAS were maximally active at higher temperatures, i.e., 45 and 50 °C, respectively. BpAS exhibited optimum pH under slightly basic conditions (pH 8.0), while BdAS, BlAS, and BtAS preferred weakly acidic conditions (pH 5.0–6.0). All BASs showed higher isomerization activities. Particularly, BlAS produced more trehalulose than turanose. Although polymerization was the highest for BtAS, BtAS synthesized α-1, 4-glucans with a lower degree of polymerization than that of the other BASs. The versatile properties of the BASs described could contribute to the efficient production of highly valuable biomaterials for the agriculture, food, and pharmaceutical industries.

Effect of different bacterial strain in probiotics on the growth performance of Nile Tilapia (Oreochromis niloticus)

The main purpose of this study was to investigate the effect of different bacterial type in probiotics on the growth performance of Nile tilapia. In this study, commercial probiotics with a different type of bacteria were added to the diet. The first group, as control, was fed with a commercial diet without probiotic. The second group was fed with commercial diet plus probiotic X contained Lactobacillus sp., Bacillus sp. and Pseudomonas sp. The third group was applied commercial diet plus probiotic Y contained Lactobacillus sp., Bacillus sp. and Bifidobacterium sp. Feeding trial was performed for 30 days. The result showed that the growth performance of Nile Tilapia including weight gain (WG) and specific growth rate (SGR) was significantly higher than the control group (P<0.05). The addition of probiotics in the commercial diet also improved feed utilization significantly. The best growth performance was obtained in the third group where the probiotic Y applied. Different bacterial strain in probiotic could give a different effect on the growth performance of Nile Tilapia because of their different action mode in fish. Therefore, the appropriate selection of probiotic strains can lead to specific advantages for species in aquaculture.

The effect of combination Bifidobacterium sp and Lactobacillus acidophilus probiotic on egg yolk cholesterol, HDL, and LDL

The purpose of this study to evaluate the effects of combination 0.5% Bifidobacterium sp.+0.5% Lactobacillus acidophilus on egg yolk cholesterol, High density lipoprotein (HDL) and low density lipoprotein (LDL) in hens. Methods: this research using completely randomized design. Sixty layers were randomized into three treatments, each treatment contains four replicates and each replicates contains five hens. Twenty five week-old Isa Brown layers were fed without probiotic (T0), 0.1% antibiotic growth promoters (T1), 0.5% Bifidobacterium sp.+0.5% L. acidophilus (T2) for 30 days. Result: the result showed that there was a significant difference between the control and all treatment groups on egg yolk cholesterol, HDL and LDL. Supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic decreased the egg yolk cholesterol and LDL (p<0.05) and increased HDL in egg yolk (p<0.05) levels. The lowest egg yolk cholesterol and LDL by supplementation 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic (155.86 and 48.46 mg/dL) respectively, compared the control (224.0200 and 58.6300 mg/dL) and the highest egg yolk HDL by supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus probiotic (50.00 mg/dL) compared the control treatment (41.5900 mg/dL). The conclusion of this study was the supplementation of 0.5% Bifidobacterium sp.+0.5% L. acidophilus could improve egg yolk quality of hens.

The Proportion of Bifidobacterium and Escherichia coli in Colon of Children with Recurrent Urinary Tract Infection.

Recurrent urinary tract infection (UTI) in children is a well-known risk factor of chronic kidney disease. Periurethral area is normally inhabited by non-pathogenic flora, such as Bifidobacterium sp., and pathogenic flora from gastrointestinal tract, such as Escherichia coli (E. coli), which can cause UTI. Dysbiosis between pathogenic and non-pathogenic bacteria leads to infections, but studies regarding dysbiosis and recurrent UTI have not yet been documented. To estimate the proportional differences between gastrointestinal E. coli and Bifidobacterium sp. in children with recurrent UTI, a cross-sectional study was conducted in children from age six months to <18 years old diagnosed with recurrent UTI in Dr. Cipto Mangunkusumo Hospital. Healthy children matched in gender and age were recruited as control group. Stool samples were obtained from all the children in the two groups. Stool DNA was extracted using real-time polymerized chain reaction method to count E. coli and Bifidobacterium sp. proportion. Children with recurrent UTI had significantly higher proportion of E. coli compared to control group (10.97 vs. 4.74; P = 0.014) and lower proportion of Bifidobacterium sp. (6.54 vs. 9.33; P = 0.594). In children with recurrent UTI group, E. coli proportion was found higher than Bifidobacterium sp. although not statistically significant (10.97 vs. 6.54; P = 0.819). In healthy controls, Bifidobacterium sp. proportion was significantly higher than E. coli (4.74 vs. 9.33; P = 0.021). The total amount of E. coli (996,004 vs. 1,099,271; P = 0.798) and Bifidobacterium sp. (835,921 vs. 1,196,991; P = 0.711) were higher in secondary UTI compared to the simple UTI. Proportion of E. coli is higher in children with recurrent UTI than in healthy children. The proportion of E. coli is higher than Bifidobacterium sp in the colon of children with recurrent UTI.

Bifidobacterium sp as Probiotic Agent - Roles and Applications

Bifidobacterium sp as Probiotic Agent - Roles and Applications

Characteristics of Snack Bar “Banaris” from Fortified Non Cereal Flour as Emergency Food for Toddler

The objective of the research is the development of banaris bar products using flour-based of local food with FIR technology, that fulfill the economic, social-cultural, nutritional and health eligibility requirements as an alternative food emergency to support diversification national food because this product is needed in accordance with the adequacy of nutrients for the age group of nutrient-prone (infants and toddlers). fortification, testing product characteristics, packaging, shelf life testing, probiotic testing of Bifidobacterium sp and Lactobacillus sp and testing of serum retinol concentrations in children under five year. The effect of giving Banaris bar products gives positive results on increasing subject height. The provision of Banaris bar products containing prebiotics can stimulate the growth of the Lactobacillus sp population in the toddler’s digestive tract in the treatment group compared to the control group toddlers.. The level of crispness, elasticity, stickiness, compactness and fragility of the banaris bar is lower than the regular bar snack. The packaging used is aluminum foil primary packaging and secondary packaging, namely carton. The packaging consists of 3 variants, namely packaging for six pieces with a size of 10.5 x 8 x 3.5 cm, packing for 9 pieces with a size of 15 x 8 x 3.5 cm and packaging for 15 pieces with a size of 15 x 13 x 3.5 cm. Banaris bar products have a shelf life of 10 months.

MODIFICATION OF OLIGOSACCHARIDE AND SHORT CHAIN FATTY ACID CONTENT OF COWPEA MILK THROUGH FERMENTATION WITH SELECTED MIXED STARTER CULTURES

Cowpea milk was fermented with three mixed starter cultures containing (i) Lactobacillus acidophilus, Bifidobacterium sp, and Streptococcus thermophilus (ABT) (ii) Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus (DT) or (iii) Lactobacillus rhamnosus GR-1 and Streptococcus thermophilus (GT). Effects of these cultures on flatulencecausing raffinose family oligosaccharides and the production of postbiotic short chain fatty acids were determined. The oligosaccharides and short chain fatty acids were determined by high performance liquid chromatography and gas chromatography, respectively. The stachyose content of raw cowpea (1.388±0.23 g/100 g) was higher than raffinose (0.221±0.06 g/100 g), while verbascose was not detected. Cowpea milk fermentation caused 67-100% reduction in raffinose and 20-70% reduction in stachyose in a culture-dependent manner. All the cultures produced propionic acid, butyric acid and valeric acid in differing concentrations but only GT and ABT produced isovaleric acid. The product fermented with DT attained 2430 ppm of propionic acid, which was four times and ten times higher than the concentrations produced by the ABT and GT cultures, respectively. In conclusion, fermentation of cowpea milk with the three starter cultures reduced flatulence-causing oligosaccharides and produced postbiotic short chain fatty acids that might promote health regardless of the survival of the microorganisms in the gut.

PENAMBAHAN PROBIOTIK LACTOBACILLUS ACIDOPHILUS DAN BIFIDOBACTERIUM SP TERHADAP ANALISIS USAHA AYAM PETELUR YANG DIINFEKSI ESCHERICHIA COLI

The purpose of this study was to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on variable cost, income and profit layers hens was infected with Escherichia coli. About 120 layers at 25 weeks of age were randomized into six treatments. The treatment consisted of factors a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The results of the study gave probiotics an effect (p 0.05) on variable cost. There was interaction between probiotic and infection factors (p 0.05) between probiotic and infection factors on variable cost. The highest variable cost was found in the treatment of non-infectious probiotics is Rp 13.594, the highest income was treated with non-infectious probiotics is Rp 26.564, the highest profit was treated with non-infectious probiotics is Rp 12.969. It could be concluded that the administration of probiotics can produce good production performance.

Exploitation of Agricultural Waste as Sole Substrate for Production of Bacterial l-Glutaminase Under Submerged Fermentation and the Proficient Application of Fermented Hydrolysate as Growth Promoting Agent for Probiotic Organisms

Microbial l-glutaminase has diverse applications, ranging from food flavouring agent to treatment of cancer. Extracellular l-glutaminase producing one soil bacterium was characterized as Acinetobacter calcoaceticus PJB1 and was grown under submerged fermentation using sugarcane bagasse as sole supporting substrate. The optimum l-glutaminase production was achieved at initial medium pH of 10.0, incubation temperature at 35 °C, inoculums concentration of 1.5% (v/v) and at 72 h. Further improvement of the enzyme production was carried out by statistical optimization procedure using response surface methodology as a result of which enzyme production was enhanced to 47.95 U/ml (2.38-fold improvement). The enzymatic degradation pattern of sugarcane bagasse was appraised using Fourier transform infrared spectroscopy and light microscope. The fermented hydrolysate was proved to be a very effective growth inducer of probiotic organism as reflected by the higher specific growth rate, volumetric growth rate and overall yield of Lactobacillus sp. and Bifidobacterium sp. This study highlights the cost effective production of l-glutaminase and simultaneous utilization of fermented waste product as growth supporting agent for health beneficial probiotic organisms.

PENGARUH PEMBERIAN PROBIOTIK Lactobacillus acidophilus DAN Bifidobacte-rium sp TERHADAP PERFORMA PRODUKSI AYAM PETELUR YANG DIINFEKSI Escherichia coli

The purpose of this study is to determine the use of probiotics Lactobacillus acidophilus and Bifidobacterium sp on feed consumption and Hen Day Production (HDP) laying hens which infected by Escherichia coli. Arround 120 layers at the age of 25 weeks are divided into six treatments. Each treatment consisted of factor a (infection and non-infection Escherichia coli) and factor b (basal feed, AGP and probiotics). The result of this study shows that giving probiotic has taken an effect (p&lt;0,05) on the feed consumption and HDP. The infection factor takes effect (p &lt;0.05) on HDP but it does not take effect (p&gt;0,05) on the feed consumption. There is interaction among probiotic factor and infection (p&gt;0.05) on HDP but it has no effect (p&gt; 0.05) on feed consumption. The highest feed consumption is found in the giving of non-infectious probiotics that is 114.7 g and the highest HDP which is from the giving of non-infectious probiotics is 98.6 %. In conclusion, the giving of probiotic Lactobacillus acidophilus and Bifidobacterium sp are able to produce well perform product and the high profit.