You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Incontinence: Evaluation (Urodynamic Testing)1 Apr 2015PD24-05 THE INTERACTION BETWEEN URINARY INCONTINENCE (UI), RECURRENT LOWER URINARY TRACT INFECTION (UTI), AND AGING IN MICE Zhina Sadeghi, Johnathan Kenyon, Albert Park, Michael Kavran, Adonis Hijaz, Thomas Hannan, and Firouz Daneshgari Zhina SadeghiZhina Sadeghi More articles by this author , Johnathan KenyonJohnathan Kenyon More articles by this author , Albert ParkAlbert Park More articles by this author , Michael KavranMichael Kavran More articles by this author , Adonis HijazAdonis Hijaz More articles by this author , Thomas HannanThomas Hannan More articles by this author , and Firouz DaneshgariFirouz Daneshgari More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1460AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES UI and recurrent UTI are both highly prevalent in the aged female population. We hypothesized that UI and UTI phenotypes have negative bidirectional impact on each other, which worsens with aging. METHODS UTI was induced by inoculations of ≈108 uropathogenic E−coli (UPEC89) in 0.05ml PBS into the bladder via a transurethral catheter. Control mock−infected mice received 0.05 ml saline. We tested various components of our hypotheses by 4 sets of experiments: i) Determined the impact of recurrent UTI on UI by measuring leak point pressure (LPP) in 20wk old B6 mice 2wk after 2 infections (1day apart) with UPEC89 (UTIx2 n=10, Saline n=6). ii) Determined the impact of UI on urine bacterial clearance in 20wk old B6 mice after UPEC89 challenge. UI was created by pudendal nerve transection in 17wk old mice, followed by single UTI induction 3wk later (PNT+UTIx1 n=10). Bacterial counted for urine collected on day 1,2,3,5,9, and 14. Control groups included: Saline n=6, UTIx1 n=10, PNT+Saline n=6; iii) Determined if recurrent UTI in >80wk old B6 mice worsens UI 2wk following infection (UTIx2 aged mice: n=15, Saline control aged mice: n=10) compared to experiment (i) 20wk old mice. iv) Determined if bacterial clearance following 2 challenges with UPEC89 was diminished in >80wk old mice compared to 20wk old mice during 2wk. RESULTS i) No significant differences between LPP of infected and control mice 2wk after inoculation. ii) Control, and PNT group remained sterile during the study. All UTIx1 induced mice, and 86% of PNT+UTIx1 mice developed UTI by day 2. Complete urine bacterial clearance rate was ≈40% lower in PNT+UTIx1 mice compared to UTIx1 alone. No LPP difference was found in PNT+UTI, saline, UTI, and PNT treated groups at day 14. iii) Significantly reduced LPP was observed in aged mice with UTIx2 compared to saline controls (P<0.05). LPPs of UTIx2 aged mice trended lower than UTIx2 and saline 20wk mice but lacked statistical significance. iv) Bacterial clearance in >80wk old mice was not observed by day 14, while 40% of 20wk old mice urine cleared by day 14. ∗Homogenized kidney tissue had negative microbial culture in all animals. CONCLUSIONS Recurrent UTI failed to induce UI in 20wk old B6 mice but was associated with increased UI in >80wk old mice. Additionally >80wk old mice cleared UTI infections slower than 20wk old mice. Our finding suggests aging prolongs bacterial clearance while prolonged bacterial infection promotes the development of UI. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e487-e488 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zhina Sadeghi More articles by this author Johnathan Kenyon More articles by this author Albert Park More articles by this author Michael Kavran More articles by this author Adonis Hijaz More articles by this author Thomas Hannan More articles by this author Firouz Daneshgari More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...