Biaxial Tests Research Articles

The impact of aging and thickness on flexural strength of various zirconia ceramics

BackgroundEffects of the aging process on the flexural strength of Y-TZP and different Y-PSZ ceramics of different thicknesses were investigated.Methods300 disc-shaped samples (12 mm diameter, 0.8 and 1.5 mm thicknesses) were made from 5 different zirconia materials 3Y-TZP LA, 4Y-PSZ, 5Y-PSZ, 3 + 5Y-PSZ and 4 + 5Y-PSZ. Experimental groups were artificially aged in an autoclave at 134 °C, 2 bar pressure for 1 and 5 h; control groups were not subjected to any treatment. Microstructural analysis was conducted using Scanning Electron Microscopy, and X-Ray Diffraction analysis determined the crystalline phase content. The impact of aging on flexural strength was investigated with the use of the biaxial flexural strength test. Data were analyzed using three-way ANOVA tests with a significance level of p < 0.05, applying Bonferroni correction for multiple comparisons.ResultsStatistically significant differences in flexural strength were observed among the materials and the material thicknesses (p < 0.05), while there were no significant differences among the aging times (p > 0.05). The highest mean flexural strength values were recorded in the case of the 3 Y-TZP-1.5 mm–5 h group (744.1 ± 61.2 MPa), which was attributed to phase-transformation toughening. The lowest values were observed in the case of the 5 Y-PSZ-1.5 mm–5 h (338.3 ± 34.8 MPa) group.ConclusionsBoth material type and thickness significantly affect the flexural strength of zirconia ceramics, whereas aging time does not; thus, material selection and thickness are crucial considerations for clinicians.

Study on crack propagation mechanism and acoustic-thermal sensitivity analysis of pre-cracked weakly cemented rock

As the intensity and depth of coal mining gradually increase, environmental disturbances become more complex, and the degree of crack development in weakly cemented surrounding rocks continues to increase. In order to study the influence mechanism of crack angle on the fracture mechanism and acoustic − thermal precursor information of weakly cemented rocks, visual biaxial loading tests were conducted on pre-cracked weakly cemented rocks based on a transparent servo loading device, combined with digital speckle correlation method (DSCM), acoustic emission (AE), and infrared thermal imaging (ITI). The research results indicate that as the inclination angle of cracks increases, the initial strength, peak strength, and elastic modulus of rocks exhibit a logarithmic relationship of first decreasing and then increasing. When the inclination angle of the crack increases from < 30° to > 75°, the failure mode of weakly cemented rocks changes from shear to tension. The propagation path of deformation localization is consistent with the propagation trajectory of surface cracks, and the extension length and time of tensile cracks are longer than those of shear cracks. The acoustic − thermal sensitivity of weakly cemented rocks with crack inclination angle of > 45° is higher than that with crack inclination angle of ≤ 30°. The research results contribute to further understanding and mastering the mechanism of crack initiation and propagation in weakly cemented rocks containing joints, and provide theoretical guidance for engineering methods to control the deformation of weakly cemented surrounding rocks.

Democratizing biomedical simulation through automated model discovery and a universal material subroutine

AbstractPersonalized computational simulations have emerged as a vital tool to understand the biomechanical factors of a disease, predict disease progression, and design personalized intervention. Material modeling is critical for realistic biomedical simulations, and poor model selection can have life-threatening consequences for the patient. However, selecting the best model requires a profound domain knowledge and is limited to a few highly specialized experts in the field. Here we explore the feasibility of eliminating user involvement and automate the process of material modeling in finite element analyses. We leverage recent developments in constitutive neural networks, machine learning, and artificial intelligence to discover the best constitutive model from thousands of possible combinations of a few functional building blocks. We integrate all discoverable models into the finite element workflow by creating a universal material subroutine that contains more than 60,000 models, made up of 16 individual terms. We prototype this workflow using biaxial extension tests from healthy human arteries as input and stress and stretch profiles across the human aortic arch as output. Our results suggest that constitutive neural networks can robustly discover various flavors of arterial models from data, feed these models directly into a finite element simulation, and predict stress and strain profiles that compare favorably to the classical Holzapfel model. Replacing dozens of individual material subroutines by a single universal material subroutine—populated directly via automated model discovery—will make finite element simulations more user-friendly, more robust, and less vulnerable to human error. Democratizing finite element simulation by automating model selection could induce a paradigm shift in physics-based modeling, broaden access to simulation technologies, and empower individuals with varying levels of expertise and diverse backgrounds to actively participate in scientific discovery and push the boundaries of biomedical simulation.

Experimental evaluation of the use of cruciform specimens for biaxial stability analysis

Novel research is proposed about the suitability of cruciform specimens for estimating the stress-strain response and buckling modes of flat laminates under equibiaxial compression. The experimental challenge lies in determining whether the specimen design and test procedure can be adequate to observe geometric instability at the coupon scale with small dimensions of the tested region. Although the literature on composite buckling is extensive, the number of studies that include experimental biaxial testing of flat laminates is limited, particularly when considering strong flexural-torsional coupling. In this work the region of interest of the cruciform specimen consists of a carbon-fibre reinforced [∓45]S laminate with shape and boundary conditions close to a clamped square plate. For the first time, the buckling mode of an anisotropic laminate is observed in the central region of a cross-shaped specimen using 3D deflection surface data recorded by Digital Image Correlation. The effect of flexural-torsional coupling at the bifurcation onset is experimentally corroborated through the orientation of the buckling mode, which is analytically utilized to quantify the proportion between bending and twist moments. Non-linearities exhibited in the stress-strain evolution of the biaxially loaded region are verified to be independent of the response of the sample's arms. Furthermore, no previous experimental investigation has shown the differences in local strain gauge measurements induced by the stress state at the ply level. Additionally, this work adapts the method for strength reduction to estimate the real critical stress of a bi-compressed laminate.

Characterization and modeling of biaxial plastic anisotropy in metallic sheets

The anisotropic behavior of cold-rolled sheet metals has been extensively studied, typically characterized by uniaxial loading tests in different directions to determine yield strengths and plastic flow (Lankford r-values). However, biaxial principal stress states often focus solely on yield loci in the RD/TD (rolling/transverse) directions, with limited studies on other loading directions. This study analyzes the relationship between the principal stress yield loci and the material principal anisotropic stress directions based on the Hill48 yield function. Biaxial tensile tests are conducted on DP590 high-strength steel using cruciform specimens cut in various directions different from the sheet RD/TD directions. The evolution of the anisotropic yield locus and plastic flow of DP590 under biaxial tensile directions beyond the traditional RD/TD anisotropic directions is revealed. Furthermore, a modified Hill48 model that considers any principal stress direction of loading is proposed, accurately describing the continuous evolution of equi-biaxial tension stress, near-plane strain stress, and plastic flow direction for different principal stress directions of loading. The issues of failing to predict plastic flow under biaxial loadings in the original Hill48 model, and the degraded representation of existing constitutive models when accounting for out-of-plane anisotropy are addressed by decoupling the relationship between anisotropic parameters and stress state. In addition, the proposed calibration strategy of anisotropic parameters, using experimental data from various principal stress directions of loading, effectively captures the anisotropic evolution caused by changes in principal stress directions. Comprehensive evaluation and verification of the plasticity model should consider yield locus for any principal stress directions of loading, and also the normal and shear planes.

Compression softening of textile CFRP reinforced concrete (CRC): Biaxial testing of cracked CRC panels and derivation of constitutive laws

Due to its insensitivity to corrosion, textile CFRP (carbon fibre reinforced polymer) reinforcement enables the construction of thin-walled concrete structures such as shells, vaults and girder webs with minimal concrete cover. In contrast to tensile and flexural behaviour, strutting and compressive membrane action of cracked thin-walled carbon reinforced concrete (CRC) elements and the interaction of compressive stresses with transverse tensile loading have not yet been investigated. While for steel reinforced concrete components, extensive biaxial testing has shown a significant reduction of concrete compressive strength as a function of the lateral tensile stress (compression softening), the effect of biaxial loading on CRC structures has not been quantified yet. However, a different behaviour is expected due to different bond properties of steel and CFRP in concrete, smaller crack spacings and the tendency of the CFRP reinforced concrete for in-plane splitting. This paper presents an extensive experimental campaign for investigation of the influence of transverse tension and cracking on the compressive strength of CRC with refined measurement techniques. Based on a literature review on compressive softening behaviour and conventional test setups for steel reinforced concrete, a novel test setup was developed. The phenomenology of compression softening in CRC as well as the main factors influencing compressive membrane behaviour are highlighted. Based on the experimental findings a constitutive law for compression softening behaviour of CRC is proposed.

Enhanced stress relaxation behavior via basal 〈a〉 dislocation activity in Zircaloy-4 cladding

This work evaluates the stress relaxation behavior of textured Zircaloy-4 cladding to understand how mechanical anisotropy influences pellet-cladding interactions. Uniaxial and biaxial stress relaxation tests are performed using full-tube axial tension and internal pressurization, respectively, aiming to achieve 0.25 %, 1 %, and 2 % equivalent strains in the cladding samples at a temperature of 300 °C. Internal pressure relaxation test results display enhanced stress relaxation compared to axial testing results, particularly for samples loaded beyond yield. Results of electron backscatter diffraction indicate increased deformation microstructure during loading and increased strain homogenization and recovery during relaxation for samples loaded via internal pressurization. Analysis indicates that the increased production and activity of basal 〈a〉 dislocations play a significant role in the enhanced relaxation measured in samples subjected to internal pressurization.

Effects of deformation-induced BCC martensitic transformation on uniaxial ductility and biaxial stretchability in metastable ferrous medium-entropy alloys

This study investigated the effects of deformation-induced martensitic transformation (DIMT) on the stretchability and fracture behavior of thin sheets made from ferrous medium-entropy alloys (MEAs). Analyses of punch load-stroke curves from Erichsen tests revealed that DIMT occurred in the 60 at% Fe MEA, 64 at% Fe MEA, and 304 stainless steel (60Fe, 64Fe, and 304SS alloys, respectively). This DIMT affects necking onset, uniform elongation, and the Erichsen index (EI) under diverse stress conditions. DIMT plays a significant role in strain hardening and mechanical instability during uniaxial tests, enhancing strain hardening through martensite formation and also initiating necking due to non-uniform deformation. Particularly in 64Fe, DIMT-induced early necking resulted in reduced uniform elongation. On the other hand, biaxial Erichsen test results demonstrated further strain hardening due to martensite-induced strain accommodation, leading to enhanced resistance to deformation during biaxial loading. The distinctive shapes of punch load-stroke curves in biaxial tests, compared to stress-strain curves from uniaxial tensile tests, are a result of the interaction between DIMT and stretching directions, as well as its alloy-dependent phase stability. In 60Fe, transformed martensite contributed to strain hardening and delayed necking initiation, while extensive martensite transformation in 64Fe enhanced strain hardening further. This understanding of the complex correlation between DIMT, strain hardening, and mechanical instability holds potential for alloy optimization and developing processing strategies, applicable to automotive manufacturing and structural engineering industries.

Load history dependency of stiffness properties of PTFE-coated glass fibre membranes

The aim of this contribution is to investigate whether the order of load ratios applied to a textile structure impacts the biaxial stiffness behaviour of the structural textile itself. These investigations are performed based on biaxial tests on an architectural woven glass fibre fabric, a representative of such membranes. The first part of the paper investigates the existence and extent of load history dependency in the material response. Biaxial test results show that effects of a previous load history on the weave geometry cannot be eliminated by further load cycling in all biaxial stress ratios. Moderate to strong effects are recognized in two stress ratios, although not in both weave directions.The second part of the paper examines how these effects influence material stiffness parameters and structural calculations. Two boundary value problems are investigated: a pressure chamber test and a roof structure. Parameters obtained by fitting biaxial tests with or without history are considered. By incorporating an adaptive load-ratio dependent scheme, highly predictive numerical results are obtained, showing that while the effect of load history may appear small in the biaxial tests and pressure chamber test, it can be significant in complex structural problems like roof structures.

Study on crack propagation characteristics of rocks with different lateral pressure based on joint monitoring of DIC and AE

During the process of rock failure, the characteristics of crack propagation affect the fracture characteristics and macroscopic mechanical behavior of rocks, indirectly affecting the safety and stability of rock engineering. In order to study the evolution characteristics of cracks during rock failure under different lateral pressure, based on an improved digital image correlation (DIC) and acoustic emission (AE) signal recognition method, a visual biaxial servo loading device was developed to conduct biaxial compression tests on mudstone with prefabricated cracks of the same inclination angle. The research results indicate that the stages of crack propagation include microcracks propagation, crack tip formation, stable macroscopic cracks propagation, and unstable macroscopic cracks propagation. As the lateral pressure increased, the initiation frequency of cracks decreased, the quantity of propagation decreased, and the propagation path shortened, indirectly increasing the bearing strength of rocks. The initiation stress, peak stress, and elastic modulus of pre-cracked rocks with lateral pressure ≤ 2 MPa were lower than those of pre-cracked rocks with lateral pressure > 3 MPa, with the minimum reduction amplitude of 14.1%, 21.2%, and 12.6%, respectively. As the lateral pressure decreased, the dispersion of the AE main frequency distribution increased and accelerated its downward expansion. The surface temperature curves of rocks were prone to fluctuations and rapid upward evolution characteristics corresponding to crack tip formation and crack propagation, respectively. The research results provide theoretical and engineering references for the mining of weak coal seams.

Mechanical behavior and energy characteristics of red sandstone with different seawater immersion heights under biaxial compression

Rock structures engineered during undersea mining are typically subjected to varying water distributions due to the motion of seawater, which considerably influences their stability. Hence, it is essential to understand the influence of seawater distribution on the mechanical behavior and energy characteristics of rocks. In this study, biaxial compression tests were conducted on red sandstone at various seawater immersion heights, and acoustic emission signals during compression were monitored. The results illustrate that the mechanical properties of the red sandstone deteriorate significantly upon seawater immersion. With an increase in seawater immersion height, the peak strength and elastic modulus of the rock specimens decreased exponentially. When the seawater immersion height was varied from 0 to 1/4 H under lateral stresses of 5, 10, 15, and 20 MPa, the peak strength decreased by 18.94%, 20.29%, 17.47%, and 14.87%, respectively, and the elastic modulus decreased by 4.6%, 8.1%, 11.7%, and 10.9%, respectively. Brittleness also decreased gradually. During compression, the acoustic emission (AE) and accumulated AE counts exhibited a stationary phenomenon, first increasing slowly and then suddenly. However, the AE counts decrease with increasing seawater immersion height. Meanwhile, with increasing seawater immersion height, the proportion of tensile cracks gradually increased and that of shear cracks gradually decreased. As the seawater immersion height increased, both the peak total input strain energy and peak total elastic strain energy decreased, whereas the peak total dissipated strain energy exhibited the opposite trend.

Experimental Protocols to Test Aortic Soft Tissues: A Systematic Review.

Experimental protocols are fundamental for quantifying the mechanical behaviour of soft tissue. These data are crucial for advancing the understanding of soft tissue mechanics, developing and calibrating constitutive models, and informing the development of more accurate and predictive computational simulations and artificial intelligence tools. This paper offers a comprehensive review of experimental tests conducted on soft aortic tissues, employing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology, based on the Scopus, Web of Science, IEEE, Google Scholar and PubMed databases. This study includes a detailed overview of the test method protocols, providing insights into practical methodologies, specimen preparation and full-field measurements. The review also briefly discusses the post-processing methods applied to extract material parameters from experimental data. In particular, the results are analysed and discussed providing representative domains of stress-strain curves for both uniaxial and biaxial tests on human aortic tissue.

Physicochemical characterization of experimental resin-based materials containing calcium orthophosphates or calcium silicate

ObjectiveTo evaluate experimental dimethacrylate-based materials containing calcium orthophosphates or calcium silicate particles in terms of their optical, mechanical and Ca2+ release behaviour. MethodsDicalcium phosphate dihydrate (DCPD), hydroxyapatite (HAp), beta-tricalcium phosphate (β-TCP) or calcium silicate (CaSi) particles were added to a photocurable BisGMA/TEGDMA resin (1:1 in mols) at a 30 vol% fraction. Materials containing silanized or non-silanized barium glass particles were used as controls. Degree of conversion (DC) at the top and base of 2-mm thick specimens was determined by ATR-FTIR spectroscopy (n = 5). Translucency parameter (TP) and transmittance (%T) were determined using a spectrophotometer (n = 3). Biaxial flexural strength (BFS) and flexural modulus (FM) were determined by biaxial flexural testing after 24 h storage in water (n = 10). Ca2+ release in water was determined during 28 days by inductively coupled plasma optical emission spectrometry (n = 3). Statistical analysis was performed using ANOVA/Tukey test (DC: two-way; TP, %T; BFS and FM: one-way; Ca2+ release: repeated measures two-way, α = 5 %). Results: CaSi and β-TCP particles drastically reduced DC at 2 mm, TP and %T (p < 0.001). Compared to both controls, all Ca2+-releasing materials presented lower BFS (p < 0.001) and only the material with DCPD showed significantly lower FM (p < 0.05). The material containing CaSi presented the highest Ca2+ release, while among materials formulated with calcium orthophosphates the use of DCPD resulted in the highest release (p < 0.001). SignificanceCaSi particles allowed the highest Ca2+ release. Notwithstanding, the use of DCPD resulted in a material with the best compromise between optical behaviour, DC, strength and Ca2+ release.

Planar biaxial testing of CXL strengthening effects

The stiffening effect of corneal crosslinking (CXL) treatment, a therapeutic approach for managing the progression of keratoconus, has been primarily investigated using uniaxial tensile experiments. However, this testing technique has several drawbacks and is unable to measure the mechanical response of cornea under a multiaxial loading state. In this work, we used biaxial mechanical testing method to characterize biomechanical properties of porcine cornea before and after CXL treatment. We also investigated the influence of preconditioning on measured properties and used TEM images to determine microstructural characteristics of the extracellular matrix. The conventional method of CXL treatment was used for crosslinking the porcine cornea. The biaxial experiments were done by an ElectroForce TestBench system at a stretch ratio of 1:1 and a displacement rate of 2 mm/min with and without preconditioning. The experimental measurements showed no significant difference in the mechanical properties of porcine cornea along the nasal temporal (NT) and superior inferior (SI) direction. Furthermore, the CXL therapy significantly enhanced the mechanical properties along both directions without creating anisotropic response. The samples tested with preconditioning showed significantly stiffer response than those tested without preconditioning. The TEM images showed that the CXL therapy did not increase the diameter of collagen fibers but significantly decreased their interfibrillar spacing, consistent with the mechanical property improvement of CXL treated samples.

Investigation of the microstructural behavior of Al–Mg–Si(X)–Mn aluminum alloys based on biaxial hot tensile tests

This study investigates the microstructural behavior of laboratory-produced Al–Mg–Si(X)–Mn aluminum alloys, focusing on the influence of varying Si content during biaxial hot tensile testing. Alloys with Si contents of 0.7%, 0.9%, and 1.3% were subjected to biaxial deformation at temperatures of 200 °C, 300 °C, and 400 °C. Using digital image correlation analysis, the impact of Si content on microstructural evolution under biaxial tensile loading was analyzed. Force–displacement analysis revealed a consistent inverse relationship between temperature and the maximum force required to initiate strain. At the temperature of 200 °C, the Al–Mg–Si(1.3)–Mn alloy required a maximum force of 1500 N, while at the temperature of 400 °C this force decreased to 900 N. The degree of anisotropy varied, with higher Si alloys exhibiting increased resistance to deformation in the transverse direction. In particular, the Al–Mg–Si(1.3)–Mn alloy showed pronounced strain anisotropy, with large major true strain φ1 values reaching up to 0.32 at 400 °C, compared to 0.26 at 300 °C and 0.2 at 200 °C. Microstructural analysis using electron backscatter diffraction (EBSD) and energy dispersive X-ray spectrometry (EDS) showed minimal changes at low temperatures, while increased dislocation density and grain boundary distortion were observed at elevated temperatures. The β-Mg2Si precipitates, influenced by Si content and temperature, significantly affected the mechanical properties. In the Al–Mg–Si(0.7)–Mn alloy, precipitates were predominantly 1–3 µm in diameter, whereas in the Al–Mg–Si(1.3)–Mn alloy, precipitates grew to 4–8 µm at higher Si content. These findings provide critical insights into the mechanical response and deformation mechanisms of aluminum alloys under biaxial tensile conditions, essential for optimizing material performance in engineering applications.Graphical abstract

An Approach to Improve Specimen Processing for the Flexural Strength Testing of Zirconia.

Measuring the flexural strength of restorative materials such as zirconia is crucial for providing proper indications for clinical applications and predicting performance. Great variations in specimen preparation for flexural strength measurements exist among laboratories. The aim was to evaluate how the processing method, surface treatment, and test method of the specimens affect the flexural strength of zirconia. Zirconia specimens (VITA YZ HT) (n = 270) were processed using CAD/CAM or were conventionally milled with three different surface treatments (machined, ground, polished) and were measured with three-point bending (non-chamfered/chamfered) or biaxial flexural strength test. Weibull statistics were conducted. The mean flexural strength values ranged from 612 MPa (conventional, machined, three-point bending non-chamfered) to 1143 MPa (CAD/CAM, polished, biaxial flexural strength). The highest reliability is achieved when specimens are prepared using thoroughly controllable processing with CAD/CAM and subsequently polished. Higher strength values are achieved with the biaxial flexural strength test method because the stress concentration in relation to the effective volume is smaller. Polishing reduces surface microcracks and therefore increases the strength values.

Investigation on the biaxial tensile testing method for metal foil using cruciform specimen

The assessment of mechanical properties of metal foil under complicated stress states poses a considerable challenge, requiring the development of new testing methods and the optimization of new specimen geometry. In this research, the biaxial tensile method and specimen shape were designed and optimized by using simulation and experiment. It is determined that the strain measurement area can be suggested as the central area of 0.6 to 0.8 times the arm width. The influence of different parameters on the tensile results of cruciform specimens was comprehensively evaluated by finite element method and multi-factor analysis. The results indicate that the number of slits markedly impacts the stress–strain uniformity and shear stress in the central region, followed by the slit length. The effect of the arm width and the slit width is comparable, more influential than the corner radius. According to the analyzed results and application requirements, a four-axis independently driven biaxial tensile test machine for small scale samples was fabricated. During the biaxial tension, the center point offset of the specimen remains within ±0.01 mm. The displacement and load synchronization errors do not exceed 0.01 mm and 50 N, respectively, facilitating the precise control of four-axis synchronization. Conclusively, cruciform specimens featuring diverse geometric characteristics were designed for industrial pure titanium, 304 stainless steel, and Inconel 718 superalloy with thickness of less than 0.3 mm. The biaxial tensile tests were performed to delineate the yield loci, revealing notable anisotropy and size effect. The validation confirms the suitability of the testing method and optimized cruciform specimens for conducting biaxial tensile mechanical properties testing on metal foils characterized by diverse types and thicknesses.

Second harmonic generation microscopy, biaxial mechanical tests and fiber dispersion models in human skin biomechanics

Advanced numerical simulations of the mechanical behavior of human skin require thorough calibration of the material’s constitutive models based on experimental ex vivo mechanical tests along with images of tissue microstructure for a variety of biomedical applications. In this work, a total of 14 human healthy skin samples and 4 additional scarred skin samples were experimentally analyzed to gain deep insights into the biomechanics of human skin. In particular, second harmonic generation (SHG) microscopy was used to extract detailed images of the distribution of collagen fibers, which were subsequently processed using a three-dimensional Fourier transform-based method recently proposed by the authors to quantify the distribution of fiber orientations. Mechanical tests under both biaxial and uniaxial loading were performed to calibrate the relevant mechanical parameters of two widely used constitutive models of soft fiber-reinforced biological tissues that account for non-symmetrical fiber dispersion. The calibration of the models allowed us to identify correlations between the mechanical parameters of the constitutive models considered. Statement of significanceConstitutive models for soft collagenous tissues can accurately reproduce the complex nonlinear and anisotropic mechanical behavior of skin. However, a comprehensive analysis of both microstructural and mechanical parameters is still missing for human skin. In this study, these parameters are determined by combining biaxial mechanical tests and SHG stacks of collagen fibers on ex vivo healthy human skin samples. The constitutive parameters are provided for two widely used hyperelastic models and enable accurate characterization of skin mechanical behavior for advanced numerical simulations.

Biaxial tension test of soft materials

Biaxial tension test of soft materials

Layer-specific biomechanical and histological properties of normal and dissected human ascending aortas

Recent studies have attempted to characterize the layer-specific mechanical and microstructural properties of the aortic tissues in either normal or pathological state to understand its structural-mechanical property relationships. However, layer-specific tissue mechanics and compositions of normal and dissected ascending aortas have not been thoroughly compared with a statistical conclusion obtained. Eighteen ascending aortic specimens were harvested from 13 patients with type A aortic dissection and 5 donors without aortic diseases, with each specimen further excised to obtain three tissue samples including an intact wall, an intima-media layer and an adventitia layer. For each tissue sample, biaxial tensile testing was performed to obtain the experimental stress-stretch ratio data, which were further fed into the Fung-type model to quantify the tissue stiffness, and Elastin Van Gieson stain and Masson's trichrome stain were employed to quantify the elastic and collagen fiber densities. Statistical analyses were performed to determine whether any significant differences exist in mechanical properties and compositions between diseased and normal aortic tissues. The tissue stiffness of intima-media samples was significant higher in diseased group than that of normal group in longitudinal direction at the stretch ratio 1.30 (p = 0.0068), while no significant differences were found in the other direction or other tissue types. Even though there was no significant difference in elastic or collagen fiber densities between two groups, the diseased group generally had lower elastic fiber density, but higher collagen fiber density for all three tissue layers. Compared to normal aortic tissues, the elastic fiber density of the intima-media layer in the dissected aortic tissue was lower, while its tissue stiffness was significantly higher, indicating the tissue stiffness of the intima-media layer could be a potential indicator for aortic dissection.